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At least 145 records · Page 8

Multispectral UAV imagery of experimental freshwater wetlands under 5 ppt saltwater intrusion, Louisiana, 2023 and 2024

Multispectral imagery was collected using an unmanned aerial vehicle (UAV) to evaluate how freshwater vegetation responds to short-term simulated saltwater intrusion events. The purpose of this data collection was to understand how plant health changes in response to acute salinity exposure, which is increasingly relevant in coastal wetland ecosystems facing sea level rise and storm surge events, such as in coastal Louisiana. Three experimental saltwater intrusions were conducted at a salinity of approximately 5 parts per thousand (ppt) for durations of 6-days, 10-days, and 17-days. UAV flights occurred both before and after each treatment. The resulting imagery was processed using Pix4DMapper software to georeference the images and generate orthomosaics. The multispectral sensor used in this study captures reflectance in five bands: blue, green, red, red-edge, and near-infrared. The uploaded data consist of georeferenced .tif orthomosaics for each spectral band, which are compatible with GIS software for vegetation analysis. This imagery can be utilized in investigations into vegetation stress, remote sensing of freshwater wetland ecosystems, and modeling of plant response to environmental changes.

EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS↗

Soil physical and chemical measurements for topsoils collected during NEON campaign in East River, CO (06/14/2018-06/28/2018)

The package is part of the DOE Watershed Function Science Focus Area (SFA) project and includes soil physical and chemical measurements from topsoils collected at the East River, Colorado, in conjunction with the National Ecological Observatory Network (NEON) Airborne Observation Platform (AOP) survey conducted in June 2018. The soil measurements include soil bulk density, soil volumetric water content, soil microbial biomass C (Carbon), N (Nitrogen) and C:N (C to N ratio), soil DNA yield, soil total extractable organic C, soil total extractable N, soil extractable nitrate, soil extractable ammonium, soil dissolved inorganic N, soil dissolved organic N, soil pH, soil TOC400 (total organic carbon at 400°C), soil ROC (residual oxidizable carbon), soil TIC (total inorganic carbon), soil TOC (total organic carbon), soil TC (total carbon), soil N, soil OM (organic matter) loss on ignition. Additional associated site metadata can be found in the ESS-DIVE package 10.15485/1618130. The dataset includes (1) 2018_NEON_soil_physical_chemical_measurements.csv: soil physical and chemical measurements indexed by soil sample IGSNs; (2) samples.csv: sample metadata file used to register International Generic Sample Numbers (IGSNs); (3) flmd.csv: file level metadata file; and (4) dd.csv: data dictionary file. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS (Catchment Hydrology and ↗

Surface water nitrogen and sediment potential nitrate reduction rates, nutrient stocks, and stable isotopes from nine wetlands at the Tanglewood Biological Station, Alabama

This dataset supports a broader study investigating wetland hydrologic and biogeochemical responses to inundation disturbances. Bimonthly surface water and sediment sampling events were conducted at nine wetland sites situated within the Tanglewood Biological Station in Alabama from April 2023 to February 2024. The contents included in the data package include surface water nitrogen (nitrogen oxides and ammonium) and sediment potential nitrate reduction rates (measured as potential denitrification and dissimilatory nitrate reduction to ammonium processing), nutrient stocks (total carbon, total nitrogen, and organic matter), and stable isotopes (carbon and nitrogen). Water level data related to each wetland location can be found at https://data.ess-dive.lbl.gov/view/doi:10.15485/2530253 (Kirker et al., 2024) and related water geochemistry data can be found at https://data.ess-dive.lbl.gov/datasets/doi:10.15485/3001967 (Forbes et al., 2025). In addition to a readme, this data package also includes a file-level metadata (FLMD) file that describes each file and a data dictionary (DD) that describes all column/row headers and variable definitions. This dataset is comprised of (1) file-level metadata; (2) data dictionary; (3) field metadata and international generic sample numbers (IGSNs); (4) readme; (5) the field protocol; and (6) a subfolder with sample data. The sample data subfolder contains (1) sediment potential denitrification rate, (2) sediment potential dissimilatory nitrate reduction to ammonium (DNRA) rate, (3) sediment total carbon and nitrogen content, (4) sediment stable isotopes (delta nitrogen-15 and delta carbon-13), (5) sediment percent organic matter, (6) surface water nitrous oxides, (7) surface water ammonium, and (8) methods codes. All files are .csv or .pdf.

Ammonium↗

UAS remote sensing (3DR SOLO platform): multispectral reflectance and normalized difference vegetation index, Seward Peninsula, Alaska, 2022

Airborne remote sensing data collected using a Parrot Sequoia+ multispectral sensor installed on a 3DR SOLO unoccupied aerial system (UAS) – operated by the Terrestrial Ecosystem Science & Technology group https://www.bnl.gov/envsci/testgroup/ at Brookhaven National Laboratory. This package includes data from 19 flights flown over the NGEE-Arctic, Kougarok Mile Marker (MM) 80, Kougarok Fire Complex (KFC) and Teller MM 27 sites in July 2022. Derived image products include point cloud, ortho-mosaiced multispectral image, a digital surface model (DSM) using the structure from motion (SfM) technique, and a normalized difference vegetation index (NDVI) map. Unprocessed and processed data products are included in this package (processing levels 0-2). Data and metadata are provided as text (*.txt, *.json, *hdr,), tabular (*.dat, *.csv), point cloud (*.laz), Cloud Optimized GeoTIFF (COG, *.tif), and image (*.jpg, *.tif, *png) formats.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

UAS remote sensing (Osprey platform): Red-green-blue (RGB) imagery, thermal infrared (TIR) imagery, and canopy reflectance, Seward Peninsula, Alaska, 2017

Airborne remote sensing data collected using the Brookhaven National Laboratory's (BNL) heavy-lift unoccupied aerial system (UAS) octocopter platform - the Osprey - operated by the Terrestrial Ecosystem Science and Technology (TEST) group (https://www.bnl.gov/testgroup). This package includes raw (L0) data from 14 flights flown over the NGEE-Arctic Council Mile Marker 71 (MM71), Kougarok MM64, and Teller MM 27 sites in August, 2017. The Osprey is a multi-sensor UAS platform that simultaneously measures very high spatial resolution optical red/green/blue (RGB) and thermal infrared (TIR) surface "skin" temperature imagery, as well as near-surface surface reflectance at 1 nm intervals in the visible to near-infrared spectral range from ~350-1000 nm measured at regular intervals along each flight path. This package provides the Level 0 (raw, unprocessed) data collected by the Osprey platform. Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Data and metadata are provided as text (*.txt, *.json), tabular (*.dat, *.csv, *.waypoint), and image (*.jpg) formats. This metadata documents contain flight campaign, platform, sensor, flight and file metadata, along with a description of the L0 data and the file naming scheme. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Specific conductivity, pH, dissolved oxygen, water temperature, alkalinity and sulfide in-situ data; Slate River floodplain, Crested Butte, CO; May 2020-October 2020

This data package includes a time-series of field measurements from May to October 2020 in groundwater and surface water from the Slate River floodplain in Crested Butte, CO, a focus field site for the SLAC Floodplain Hydro-Biogeochemistry SFA. The data was generated as part of the work targeting the overarching research question for the SLAC SFA: How do ubiquitous subsurface interfaces mediate molecular-scale biogeochemical processes and groundwater quality in floodplains and watersheds? The data packade includes 6 data files, one for each measured variable: specific conductivity, pH, dissolved oxygen, water temperature, alkalinity and sulfide. All measurements were recorded in the field immediately after water sampling. Groundwater samples were extracted from a network of installed rhizons (Rhizosphere Research Products, part no. 19.60.21F, 0.6 micrometer mesh size) and piezometer wells within the floodplain. In addition to the data files, there is a terminology file explaining the terms used, a file level metadata file, and a sensor file with metadata about the sensors used.All files are in csv format.

54 ENVIRONMENTAL SCIENCES↗

Specific conductivity, pH, dissolved oxygen, water temperature and alkalinity in-situ data; Slate River floodplain, Crested Butte, CO; March 2021-October 2021

This data package includes a time-series of field measurements from March to October 2021 in groundwater and surface water from the Slate River floodplain in Crested Butte, CO, a focus field site for the SLAC Floodplain Hydro-Biogeochemistry SFA. The data was generated as part of the work targeting the overarching research question for the SLAC SFA: How do ubiquitous subsurface interfaces mediate molecular-scale biogeochemical processes and groundwater quality in floodplains and watersheds? The data package includes 5 data files, one for each measured variable: specific conductivity, pH, dissolved oxygen, water temperature and alkalinity. All measurements were recorded in the field immediately after water sampling. Groundwater samples were extracted from a network of installed rhizons (Rhizosphere Research Products, part no. 19.60.21F, 0.6 micrometer mesh size) and piezometer wells within the floodplain. In addition to the data files, there is a terminology file explaining the terms used, a file level metadata file, and a sensor file with metadata about the sensors used.All files are in csv format.

54 ENVIRONMENTAL SCIENCES↗

Total metals, sulfur and organic carbon data; Slate River floodplain, Crested Butte, CO; March 2021-October 2021

This data package includes processed and undiluted measurements for metal, sulfur and organic carbon concentrations from pore water (groundwater) samples from the Slate River floodplain of Crested Butte, CO, a focus field site for the SLAC Floodplain Hydro-Biogeochemistry SFA. The data was generated as part of the work targeting the overarching research question for the SLAC SFA: How do ubiquitous subsurface interfaces mediate molecular-scale biogeochemical processes and groundwater quality in floodplains and watersheds? Samples were collected between March and October of 2021. These measurements were all recorded at the Environmental Measurements Facility (EM-1) at Stanford University in Stanford, CA. Groundwater samples were extracted from a network of installed rhizon (Rhizosphere Research Products, part no. 19.60.21F, 0.6 micrometer mesh size) and piezometer wells within the river floodplain. All water samples were shaded from sun exposure during extraction from the subsurface and preserved at 2C until measured at EM-1.Analysis by ICP-MS:Measurements for total metals were performed on an inductively coupled plasma optical emission spectrometer (ICP-OES; iCAP 6300, Thermo Scientific, Cambridge, U.K.). Calibration standards were prepared from the mulit element stock solution (Sigma-Aldrich Multielement standard for ICP, St. Louis, MO) using matrix matched to sample solutions (2% HNO3). Calibration curves included 5 points with correlation coefficients of >0.99. The QC protocol includes a continuing calibration blank and quality control samples that are analyzed just after calibration and again every 20 samples and at the completion of the run. Acceptable QC responses must be between 90-110% of the certified value. Analysis by Total Organic Carbon:Dissolved organic carbon concentrations were quantified on a total organic carbon (TOC) analyzer (TOC-L, Shimadzu, Kyoto, Japan) running the NPOC method. Standard curves were developed using an Organic Carbon Standard from RICCA Chemical Company (Arlington,TX). A series of 4 to 6 standards were automatically diluted by the instrument in a concentration range that spans that of the samples. A blank sample was run just after the calibration curve and at the end of the run. A QC sample was run every 25 samples. Acceptable QC responses must be between 90-110% of the certified value.All files are in csv format.

54 ENVIRONMENTAL SCIENCES↗

In-situ electrochemical and water quality data; Slate River and East River floodplains, Crested Butte, CO; May 2022-September 2022

This data package includes a time-series of field measurements from May to September 2022 in groundwater and surface water from the Slate River and East River floodplains in Crested Butte, CO, a focus field site for the SLAC Floodplain Hydro-Biogeochemistry SFA. The data was generated as part of the work targeting the overarching research question for the SLAC SFA: How do ubiquitous subsurface interfaces mediate molecular-scale biogeochemical processes and groundwater quality in floodplains and watersheds? The data package includes 5 data files, one for each measured variable: specific conductivity, pH, dissolved oxygen, water temperature and alkalinity. All measurements were recorded in the field immediately after water sampling. Groundwater samples were extracted from a network of installed rhizons (Rhizosphere Research Products, part no. 19.60.21F, 0.6 micrometer mesh size) and piezometer wells within the floodplain. In addition to the data files, there is a terminology file explaining the terms used, a file level metadata file, and a sensor file with metadata about the sensors used.All files are in csv format.

54 ENVIRONMENTAL SCIENCES↗

Data and scripts associated with a manuscript investigating impacts of solid phase extraction on freshwater organic matter optical signatures and mass spectrometry pairing

This data package is associated with the publication “Investigating the impacts of solid phase extraction on dissolved organic matter optical signatures and the pairing with high-resolution mass spectrometry data in a freshwater system” submitted to “Limnology and Oceanography: Methods.” This data is an extension of the River Corridor and Watershed Biogeochemistry SFA’s Spatial Study 2021 (https://doi.org/10.15485/1898914). Other associated data and field metadata can be found at the link provided. The goal of this manuscript is to assess the impact of solid phase extraction (SPE) on the ability to pair ultra-high resolution mass spectrometry data collected from SPE extracts with optical properties collected on ambient stream samples. Forty-seven samples collected from within the Yakima River Basin, Washington were analyzed dissolved organic carbon (DOC, measured as non-purgeable organic carbon, NPOC), absorbance, and fluorescence. Samples were subsequently concentrated with SPE and reanalyzed for each measurement. The extraction efficiency for the DOC and common optical indices were calculated. In addition, SPE samples were subject to ultra-high resolution mass spectrometry and compared with the ambient and SPE generated optical data. Finally, in addition to this cross-platform inter-comparison, we further performed and intra-comparison among the high-resolution mass spectrometry data to determine the impact of sample preparation on the interpretability of results. Here, the SPE samples were prepared at 40 milligrams per liter (mg/L) based on the known DOC extraction efficiency of the samples (ranging from ~30 to ~75%) compared to the common practice of assuming the DOC extraction efficiency of freshwater samples at 60%. This data package folder consists of one main data folder with one subfolder (Data_Input). The main data folder contains (1) readme; (2) data dictionary (dd); (3) file-level metadata (flmd); (4) final data summary output from processing script; and (5) the processing script. The R-markdown processing script (SPE_Manuscript_Rmarkdown_Data_Package.rmd) contains all code needed to reproduce manuscript statistics and figures (with the exception of that stated below). The Data_Input folder has two subfolders: (1) FTICR and (2) Optics. Additionally, the Data_Input folder contains dissolved organic carbon (DOC, measured as non-purgeable organic carbon, NPOC) data (SPS_NPOC_Summary.csv) and relevant supporting Solid Phase Extraction Volume information (SPS_SPE_Volumes.csv). Methods information for the optical and FTICR data is embedded in the header rows of SPS_EEMs_Methods.csv and SPS_FTICR_Methods.csv, respectively. In addition, the data dictionary (SPS_SPE_dd.csv), file level metadata (SPS_SPE_flmd.csv), and methods codes (SPS_SPE_Methods_codes.csv) are provided. The FTICR subfolder contains all raw FTICR data as well as instructions for processing. In addition, post processed FTICR molecular information (Processed_FTICRMS_Mol.csv) and sample data (Processed_FTICRMS_Data.csv) is provided that can be directly read into R with the associated R-markdown file. The Optics subfolder contains all Absorbance and Fluorescence Spectra. Fluorescence spectra have been blank corrected, inner filter corrected, and undergone scatter removal. In addition, this folder contains Matlab code used to make a portion of Figure 1 within the manuscript, derive various spectral parameters used within the manuscript, and used for parallel factor analysis (PARAFAC) modeling. Spectral indices (SPS_SpectralIndices.csv) and PARAFAC outputs (SPS_PARAFAC_Model_Loadings.csv and SPS_PARAFAC_Sample_Scores.csv) are directly read into the associated R-markdown file. We acknowledge the Yakama Nation as owners and caretakers of the lands where we collected some of these data. We thank the Confederated Tribes and Bands of the Yakama Nation Tribal Council and Yakama Nation Fisheries for working with us to facilitate sample collection and optimization of data usage according to their values and worldview.

54 ENVIRONMENTAL SCIENCES↗

Photosynthetic and stomatal response, and leaf traits, of two pine species, Talladega National Forest, Alabama, 2023

Photosynthetic response to irradiance (AQ), CO2 (ACi) and stomatal response to irradiance measurements on two pine species common to the southeast United States, long leaf pine, Pinus palustris Mill., and loblolly pine, Pinus taeda L.. Data were collected in June 2023 within the Oakmulgee District of the Talladega National Forest in west-central Alabama at the NEON site (TALL). Measurements were made with four LI-COR LI-6800 and one LI-COR LI-6400XT gas exchange systems. ACi and AQ curves enable the estimation of photosynthetic parameters (e.g., Vcmax, Jmax, Rd, aQY), while stomatal response curves enable the estimation of stomatal slope and intercept parameters. Foliar trait data (needle dimensions, leaf mass per area, leaf nitrogen concentration) is provided for all gas exchange samples. The data package files include multiple raw and processed data files (csv), metadata files (csv), the complete instrument output for all measurements in .zip format, and a pdf describing the experimental protocol.

54 ENVIRONMENTAL SCIENCES↗

Plant physiology, shrub size, thaw depth and soil water content, Seward Peninsula, Alaska, 2023

Stomatal response to changing irradiance measurements (m curves), CO2 response (ACi) and light response (AQ) curves on common tussock tundra shrub, graminoid and forb species. Data were collected in July 2023 from three sites on the Seward Peninsula, Kougarok Mile 64, Teller Mile 27 and Council. Measurements were made with LI-COR LI-6800 gas exchange systems on 18 species. For samples from Teller and Kougarok, thaw depth, soil moisture content and dGPS locations are also provided for most samples. Canopy height and canopy diameter are provided for shrubs measured at the Teller site. The data package files include data files, metadata files and the complete instrument output for all gas exchange measurements in .csv format, and a pdf describing the experimental protocols. See the related data package NGA508 for foliar trait data (leaf mass per area, leaf nitrogen concentration).The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a 15-year research effort (2012-2027) to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Organic layer thickness and carbon concentration in burned and unburned sites, Seward Peninsula, AK, 2022

Measurements associated with organic layer samples collected from naturally burned (1971, 2002, 2015, 2019) and unburned sites at the Kougarok Fire Complex, Seward Peninsula, AK, 2022. Here, a discontinuous permafrost underlies an arctic tundra ecosystem. Measurements include elemental carbon and nitrogen concentrations and stocks, organic layer thickness, and thaw depth. There are five files in *.csv format with one data file and four data description files including data dictionary, methods, terminology, and file-level metadata. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Levoglucosan data from five coastal streams impacted by the 2020 CZU Lightning Complex Fires, California, United States

This dataset includes levoglucosan data for five coastal California (United States) streams impacted by the 2020 CZU Lightning Complex Fires which burned from August 16th through September 22nd. Levoglucosan is a highly soluble and biolabile fraction of pyrogenic carbon. The five watersheds (San Lorenzo River, Pescadero Creek, Majors Creek, Laguna Creek, and Scott Creek) were impacted by the fires with watersheds experiencing a range of burn severity and extents. Grab samples were collected from each stream between October 2020 and May 2021, targeting both baseflow and event flow hydrologic conditions. Additional biogeochemistry data (i.e., organic and black carbon concentrations) can be found in a separate data package (https://doi.org/10.4211/hs.421c0226bb38460c8393d67fe0c4f802). This data package consists of one main data folder that contains (1) readme; (2) file-level metadata; (3) data dictionary; (4) field metadata with international generic sample numbers (IGSN); (5) methods codes; and (6) levoglucosan data. All files are .csv or .pdf.

2020 CZU Lightning Complex Fires↗

Organic Matter Composition in June 2023 and September 2023 Across the McKenzie Sub-Basin Impacted by the 2020 Holiday Farm Fire

This dataset represents results from a field study aiming to understand the variability in post-fire responses of dissolved organic matter and determine drivers of post-fire responses. Samples were collected at 58 sites within the McKenzie River Watershed (Oregon, USA) that were upstream, within, and downstream of the Holiday Farm Fire burn perimeter. The samples were collected in June 2023 and September 2023 during storm events, approximately 3 years post-fire. Samples were characterized for benezenepolycarboxylic acids (BPCA) and ultra-high resolution mass spectrometry. Dissolved organic carbon and optics (absorbance and fluorescence) data can be found in a separate data packages (https://ir.library.oregonstate.edu/concern/datasets/zc77sz60m, https://ir.library.oregonstate.edu/concern/datasets/mc87q034m). Related data from a subset of sites from 2020-2022 can be found at https://data.ess-dive.lbl.gov/datasets/doi:10.15485/1869708 and https://data.ess-dive.lbl.gov/datasets/doi:10.15485/2478546. For details on how to navigate data packages generated by this project, see https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About. This dataset contains (1) file-level metadata; (2) data dictionary; (3) data package readme; (4) metadata; (5) methods information; (6) benzene polycarboxylic acid (BPCA) concentration data; (7) Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) methods; (8) folder of high resolution characterization of organic matter via 12 Tesla FTICR-MS data generated through the Environmental Molecular Sciences Laboratory (EMSL; https://www.pnnl.gov/environmental-molecular-sciences-laboratory). This package contains the following file types: csv, xml, pdf.

54 ENVIRONMENTAL SCIENCES↗

Data from Stewart et al. 2026 "Organic Colloid Composition in Variable-Redox Porewaters within a Mountainous Floodplain"

Redox gradients, often driven by changes in sediment moisture levels in porous, heterogeneous groundwater systems, create dynamic conditions that may promote the production and transport of colloids within natural waters. While much research has focused on the inorganic composition of colloids, the organic composition remains less well understood. Organic matter (OM) in colloids may associate with minerals, complex metal ions, and serve as an electron donor for microbial respiration; therefore, its composition is of high interest. We examined the composition of porewater OM along a redox gradient in a riparian soil located along the Slate River in Crested Butte, Colorado, USA as a function of depth (90, 130, 200, and 350 cm below ground surface). All depths were oxic to suboxic, except 200 cm, where the products of iron and sulfate reduction were observed concomitant with an increase in dissolved and/or colloidal OM, pH, alkalinity, and conductivity. We investigated the composition of porewater using correlated scanning transmission X-ray microscopy and transmission electron microscopy. We observed a change in the OM chemistry from carboxylate-rich at the 200 cm depth (where it was also enmeshed with non-crystalline iron) to phenol- and substituted-aromatic-rich at other depths. Radiocarbon dating revealed carbon in porewater at 200 cm was younger than depths above and below. Soil porewater can flow down into the underlaying gravel bed during baseflow conditions, thus we speculate whether riparian porewater could transport iron- and carboxylate-rich organic colloids into surrounding surface waters through the gravel bed conduit. This data package includes geochemical data (.csv) as a function of depth from porewaters in OBJ1. Measurements included are pH, conductivity, TOC, Fe concentration, sulfate concentration, dissolved oxygen. Additionally radiocarbon dating age of carbon in samples is provided. Additionally, this dataset contains metadata files (.csv) describing the methods, file contents, and column headers.

alpine floodplain↗

Plant Physiology, Alder and Willow Species, Seward Peninsula, Alaska, 2019

Photosynthetic parameters (Vcmax and Jmax), CO2 response (ACi) curves and dark respiration (Rdark) measurements of tundra shrub species. Data were collected at the NGEE-Arctic Kougarok and Teller study sites on the Seward Peninsula, AK, USA in July 2019. Four species were measured using LI-COR LI-6400 portable gas exchange systems: Alnus viridis ssp. fruticosa, Salix richardsonii and Salix pulchra. The data package files include data files, metadata files, dGPS locations and the complete instrument output for all gas exchange measurements in .csv format. See the related data packages for foliar trait data (leaf mass per area, leaf nitrogen concentration), and leaf spectral reflectance data collected simultaneously on the same shrubs (NGA210, NGA212).The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Stomatal response of three Arctic plant species, Seward Peninsula, Alaska, 2022

Stomatal response to changing irradiance measurements on three common tussock tundra shrub species. Data were collected in 2022 from two sites on the Seward Peninsula, Kougarok Mile 64 and Teller Mile 27. Measurements were made with LI-COR LI-6800 gas exchange systems using a slow curve light response method. Light curve observations enable the estimation of stomatal slope and intercept parameters, g1 and g0. The data package files include a data file, metadata files and the complete instrument output for all measurements in .csv format, and a pdf describing the experimental protocol. See the related data package NGA315 for foliar trait data (leaf mass per area, leaf nitrogen concentration) and NGA209 for co-located thaw depth and soil moisture. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗