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At least 145 records · Page 8

Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing

Peptide loading of major histocompatibility complex class I (MHC-I) molecules is central to antigen presentation, self-tolerance, and CD8 + T-cell activation. TAP binding protein, related (TAPBPR), a widely expressed tapasin homolog, is not part of the classical MHC-I peptide-loading complex (PLC). Using recombinant MHC-I molecules, we show that TAPBPR binds HLA-A*02:01 and several other MHC-I molecules that are either peptide-free or loaded with low-affinity peptides. Fluorescence polarization experiments establish that TAPBPR augments peptide binding by MHC-I. The TAPBPR/MHC-I interaction is reversed by specific peptides, related to their affinity. Mutational and small-angle X-ray scattering (SAXS) studies confirm the structural similarities of TAPBPR with tapasin. These results support a role of TAPBPR in stabilizing peptide-receptive conformation(s) of MHC-I, permitting peptide editing.

60 APPLIED LIFE SCIENCES↗

Fermilab Muon Campus g-2 Cryogenic Distribution Remote Control System

The Muon Campus (MC) is able to measure Muon g-2 with high precision and comparing its value to the theoretical prediction. The MC has four 300 KW screw compressors and four liquid helium refrigerators. The centerpiece of the Muon g-2 experiment at Fermilab is a large, 50-foot-diameter superconducting muon storage ring. This one-of-a-kind ring, made of steel, aluminum and superconducting wire, was built for the previous g-2 experiment at Brookhaven. Due to each subsystem has to be far away from each other and be placed in the distant location, therefore, Siemens Process Control System PCS7-400, Automation Direct DL205 & DL05 PLC, Synoptic and Fermilab ACNET HMI are the ideal choices as the MC g-2 cryogenic distribution real-time and on-Line remote control system. This paper presents a method which has been successfully used by many Fermilab distribution cryogenic real-time and On-Line remote control systems.

43 PARTICLE ACCELERATORS↗

In-Containment Signal Conditioning and Transmission via Power Lines within High Dose Rate Areas of Nuclear Power Plants

Signal conditioning and transmission for sensor systems and networks within the containment of nuclear power plants (NPPs) still poses a challenge to engineers, particularly in the case of equipment upgrades for existing plants, temporary measurements, decommissioning of plants, but also for new builds. This paper presents an innovative method for efficient and cost-effective instrumentation within high dose rate areas inside the containment. A transmitter-receiver topology is proposed that allows simultaneous, unidirectional point-to-point transmission of multiple sensor signals by superimposing them on existing AC or DC power supply cables using power line communication (PLC) technology. Thereby the need for costly installation of additional cables and containment penetrations is eliminated. Based on commercial off-the-shelf (COTS) electronic parts, a radiation hard transmitter is designed to operate in harsh environment within the containment during full plant operation. Hardware modularity of the transmitter allows application specific tradeoffs between redundancy and channel bandwidth. At receiver side in non-radiated areas, signals are extracted from the power line, demodulated, and provided either in analog or digital output format. Laboratory qualification tests and field test results within a boiling water reactor (BWR) are validating the proof of concept of the proposed system. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Simulation-Based Parameter Optimization Framework for Large-Scale Hybrid Smart Grid Communications Systems Design

The design of reliable, dynamic, fault-tolerant hybrid smart grid communication networks is a challenge to achieve for autonomous power grids. Hybrid networks use different communications technologies for different area networks. A simulation-based parameter optimization framework is proposed to tune parameters of hybrid communication technologies to achieve the optimal network performance. It consists of three main components: a parallel executor used to speedup a list of simulations; a sampler running simulations using the parallel executor at each generation; and a hybrid stochastic optimization algorithm for tuning configurable parameters of hybrid designs and applications. The proposed hybrid metaheuristic optimization algorithm combines an evolutionary algorithm with a gradient method to quickly achieve an approximately global optimum solution. Three optimization test functions are employed to train the adjustable parameters of the hybrid algorithm. Results show the proposed parameter optimization framework can help the designer choose the right hybrid architecture with an optimal parameter set for a large-scale broadband PLC-WiMAX hybrid smart grid communication network.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Detecting Hardware Trojans in PCBs Using Side Channel Loopbacks

Malicious modifications to printed circuit boards (PCBs) are known as hardware Trojans. These may arise when malafide third parties alter PCBs premanufacturing or postmanufacturing and are a concern in safety-critical applications, such as industrial control systems. In this research, we examine how data-driven detection can be utilized to detect such Trojans at run-time. We develop a flexible and reconfigurable PCB test bed derived from the popular open-source programmable logic controller (PLC) platform “OpenPLC.” We then develop a Trojan detection framework, which utilizes and analyzes multimodal side channels (e.g., timing, magnetic signals, power, and hardware performance counters). We consider defender-configurable input/output (I/O) loopback test, comparison with design-document baselines, and magnetometer-aided monitoring of system behavior under defender-chosen excitations. Our approach can extend to golden-free environments. Golden (known-good) versions of the PCBs are assumed not available, but design information, datasheets, and component-level data are available. We demonstrate the efficacy of our approach on a range of Trojans instantiated in the test bed.

42 ENGINEERING↗

Size-related decline in dryland shrubs is related to reductions in hydraulic efficiency and carbon assimilation and not nonstructural carbohydrate depletion

Plant growth and survival are fundamentally constrained by water transport from roots to leaves, impacting carbon assimilation and associated labile carbon pools. However, physiological constraints on growth and survival vary with plant age, due to changes in metabolic sinks, and increases in hydraulic path length from rhizosphere to canopy. We investigated crown dieback, growth, hydraulics, carbon assimilation and carbohydrate storage in relation to increasing basal diameter of two dominant shrub species (Caragana korshinskii and Artemisia ordosica) at the southeastern edge of the Tengger Desert, China. The aim was to identify mechanisms of decreased performance with plant size in dryland shrubs. Clear contrasts in stomatal regulation of leaf water potentials were detected between species. Despite these contrasts, radial growth, hydraulic transport efficiency (Ks), and photosynthetic efficiency similarly declined in both species with increasing plant size, while non-structural carbohydrate (NSC) reserves remained unchanged. Xylem embolism (PLC) increased with plant size, resulting in significant reductions in carbon assimilation in both species. Results indicate that hydraulic, and potentially carbon assimilation constraints, rather than reductions in carbohydrate storage, govern growth-related dryland shrub decline. These findings improve our understanding of how population demography impacts dryland forest response to climate change.

Zhang, Hongxia↗

Blue Keanu: A Scientific Visualization Tool For Network Data

This software allows the user to visualize complex PCAP-ng files captured from network capture software such as Wireshark. The visualization runs in a GUI window that can be zoomed or moved to areas of interest in a waterfall type display. The user then can see an area of interest that looks different than the typical traffic visually, such as a human interaction or non-repetitive area of data. The program will tell the user the packet number and byte offset of interest for fast analysis of discrete atomic or non-random events. This is particularly useful for visualization of unknown binary format data, such as in PLC or SCADA protocols that may have human or other non-repetitive activity for further analysis, reverse engineering, or fast forensic analysis.

Durller, MichaelGeorge↗

CDL2PLC translator v0.1.0

The CDL-PLC translator aims at translating control sequences for building energy systems from the CDL CXF format to the PLCopen XML format. The CDL CXF developed at LBL within the OpenBuildingControl project, and now being standardized via ASHRAE Standard 231P, enables expressing control sequences developed in the simulation environment Modelica in a JSON format. The PLCopen XML is an existing exchange format standardized in IEC 61131-10 for Programmable Logic Controllers (PLCs) following the IEC 61131 standard as one target system of CDL among others. The translation from the CDL CXF to the PLCopen XML contributes to a seamless workflow from the model-based development of control sequences in simulation environments, which is not building practice today, and their digital implementation on building controllers, which replaces graphical and textual documents used for this purpose today. The translator is at a prototypical stage and enables, as a proof of concept, the translation of very simple control sequences composed of 4 selected function blocks out of 137 function blocks defined in CDL. The translation includes the connection of inputs and outputs of function blocks and the expression of a control function in CDL to the equivalent code in IEC 61131-3.

Walther, Karl↗

Structural Insights into Neutrophilic Migration Revealed by the Crystal Structure of the Chemokine Receptor CXCR2 in Complex with the First PDZ Domain of NHERF1

Neutrophil plays an essential role in host defense against infection, but uncontrolled neutrophilic infiltration can cause inflammation and severe epithelial damage. We recently showed that CXCR2 formed a signaling complex with NHERF1 and PLC-2, and that the formation of this complex was required for intracellular calcium mobilization and neutrophilic transepithelial migration. To uncover the structural basis of the complex formation, we report here the crystal structure of the NHERF1 PDZ1 domain in complex with the C-terminal sequence of CXCR2 at 1.16 Å resolution. The structure reveals that the CXCR2 peptide binds to PDZ1 in an extended conformation with the last four residues making specific side chain interactions. Remarkably, comparison of the structure to previously studied PDZ1 domains has allowed the identification of PDZ1 ligand-specific interactions and the mechanisms that govern PDZ1 target selection diversities. In addition, we show that CXCR2 can bind both NHERF1 PDZ1 and PDZ2 in pulldown experiments, consistent with the observation that the peptide binding pockets of these two PDZ domains are highly structurally conserved. The results of this study therefore provide structural basis for the CXCR2-mediated neutrophilic migration and could have important clinical applications in the prevention and treatment of numerous neutrophil-dependent inflammatory disorders.

59 BASIC BIOLOGICAL SCIENCES↗

RivGen Controller Performance Raw Data, Igiugig, AK

Contains raw data for operations of Ocean Renewable Power Company (ORPC) RivGen Power System in Igiugig 2015 in Matlab data file format. Two data files capture the data and timestamps for data, including power in, voltage, rotation rate, and velocity. Location, Description, Format, Column 1, Date and time, MM/DD/YYYY, hh:mm:ss, Column 2, DC voltage (after DC filter), VDC, Column 3, DC current into inverters, IDC, Column 4, DC current into secondary resistive load, IDC, Column 5, Power into inverters, kW, Column 6, Power into secondary load, kW, Column 7, Frequency of single phase AC voltage from generator, Hz, Column 8, rotation rate of generator/turbine (rested at 1.1 when stationary), rad/s, Column 9, Velocity Algorithm 2, m/s, Column 10, Velocity Algorithm 3, m/s, Column 11, Rcodes for secondary load, see document "SL Controller PLC Memory", Column 12, Voltage measured between phase A and B, Vll,

16 TIDAL AND WAVE POWER↗

LCLS-II Cryomodule and Cryogenic Distribution Control

LCLS-II is a superconducting upgrade to the existing Linear Coherent Light Source at the SLAC National Accelerator Laboratory. Construction is underway with a planned continuous wave beam rate of up to 1 MHz. Two cryogenic plants provide helium to a distribution system, and 37 cryomodules with superconducting cavities will operate with Liquid helium at 2.2K. The cryomodules and distribution system is controlled with networked PLC's and EPICS as an integrated system that work in concert for controlling valves, pressure, flow, and temperature. Interlocks and critical process information is communicated with the Low Level Radio Frequency, vacuum, and magnet systems. Engaging the controls community proved vital in advancing the controls architecture from a conventional design to a centralized, reliable, and cost-effective distributed platform.

43 PARTICLE ACCELERATORS↗

LCLS-II Cryomodule and Cryogenic Distribution Control

The new superconducting Linear Coherent Light Source (LCLS-II) at the SLAC National Accelerator Laboratory will be an upgrade to LCLS, the world’s first hard X-ray free-electron laser. LCLS-II is in an advanced stage of construction with equipment for both Cryoplants as well as more than half of the 37 cryomodules onsite. Jefferson Lab (JLab) is a partner lab responsible for building half of the LCLS-II cryomodules. Hence the Low Energy Recirculation Facility (LERF) at JLab was used to stage and test LCLS-II cryomodules before shipping them to SLAC. LERF was set up to test two cryomodules at a time. LERF used LCLS-II cryogenic controls instrumentation racks, Programmable Logic Controllers (PLC) controls and Experimental Physics and Industrial Control System (EPICS) Input/Output Controllers (IOCs) with the intention to use the LERF setup to check-out and verify cryogenic controls for LCLS-II. The cryogenic controls first utilized at LERF would then be replicated for controlling all 37 cryomodules via an EPICS user interface. This paper discusses the cryogenic controls currently developed for implementation in the LCLS-II project.

43 PARTICLE ACCELERATORS↗

Analog Signal Multiplexing System for the Iota Proton Injector

The Fermilab Accelerator Science and Technology (FAST) Facility at FNAL is a dedicated research and development center focused on advancing particle accelerator technologies for future applications worldwide. Currently, a key objective of FAST Operations is to commission the 2.5 MeV IOTA Proton Injector (IPI) and enable proton injection into the Integrable Optics Test Accelerator (IOTA) storage ring. The low and medium-energy sections of the IPI include four frame-style dipole trims and two multi-function correctors with independently controlled coils, requiring readout of 32 analog channels for current and voltage monitoring in total. To reduce cost and optimize rack space within the PLC-based control system, a 32-to-4 analog signal multiplexing system was designed and implemented. This system enables real-time readback of excitation parameters from all magnetic correctors. This paper presents the design, construction, implementation, and performance of the multiplexing system.

MacLean, Daniel R. [Fermilab] (ORCID:0000000210103↗

Refinery burner simulation design architecture summary.

This report describes the architectural design for a high fidelity simulation of a refinery and refinery burner, including demonstrations of impacts to the refinery if errors occur during the refinery process. The refinery burner model and simulation are a part of the capabilities within the Sandia National Laboratories Virtual Control System Environment (VCSE). Three components comprise the simulation: HMIs developed with commercial SCADA software, a PLC controller, and visualization software. All of these components run on different machines. This design, documented after the simulation development, incorporates aspects not traditionally seen in an architectural design, but that were utilized in this particular demonstration development. Key to the success of this model development and presented in this report are the concepts of the multiple aspects of model design and development that must be considered to capture the necessary model representation fidelity of the physical systems.

99 GENERAL AND MISCELLANEOUS↗

Target Diagnostics Supports NIF's Path to Ignition

The physics requirements derived from the National Ignition Facility (NIF) experimental campaigns are leading to a wide variety of target diagnostics. Software development for the control and analysis of these diagnostics is included in the NIF Integrated Computer Control System, Diagnostic Control System and Data Visualization. These projects implement the configuration, controls, data analysis and visual representation of most of these diagnostics. To date, over 40 target diagnostics have been developed to support NIF experiments. In 2011 diagnostics were developed or enhanced to measure Ignition performance in a high neutron yield environment. Performance is optimized around four key variables: Adiabat (a) which is the strength and timing of four shocks delivered to the target, Velocity (V) of the imploding target, Mix (M) is the uniformity of the burn, and the Shape (S) of the imploding Deuterium Tritium (DT) hot spot. The diagnostics used to measure each of these parameters is shown in figure 1. Adiabat is measured using the Velocity Interferometer System for Any Reflector (VISAR) diagnostic consisting of three streak cameras. To provide for more accurate adiabat measurements the VISAR streak cameras were enhanced in FY11 with a ten comb fiducial signal controller to allow for post shot correction of the streak camera sweep non-linearity. Mix is measured by the Neutron Time of Flight (NTOF) and Radiochemical Analysis of Gaseous Samples (RAGS) diagnostics. To accommodate high neutron yield shots, NTOF diagnostic controls are being modified to use Mach Zehnder interferometer signals to allow the digitizers to be moved from near the target chamber to the neutron shielded diagnostic mezzanine. In December 2011 the first phase of RAGS diagnostic commissioning will be completed. This diagnostic will analyze the tracers that are added to NIF target capsules that undergo nuclear reactions during the shot. These gases are collected and purified for nuclear counting by the RAGS system. Three new instrument controllers were developed and commissioned to support this diagnostic. A residual-gas analyzer (RGA) instrument measures the gas content at various points in the system. The Digital Gamma Spectrometer instrument measures the radiological spectrum of the decaying gas isotopes. A final instrument controller was developed to interface to a PLC based Gas collection system. In order to support the implosion velocity measurements an additional Gated X-ray Detector (GXD) diagnostic was tested and commissioned. This third GXD views the target through a slit contained in its snout and allows the other GXD diagnostics to be used for measuring the shape on the same shot. In order to measure the implosion shape in a high neutron environment, Actide Readout In A Neutron Environment (ARIANE) and Neutron Imaging (NI) diagnostics were commissioned. The controls for ARIANE, a fixed port gated x-ray imager, contain a neutron shielded camera and micro channel plate pulser with its neutron sensitive electronics located in the diagnostic mezzanine. The NI diagnostic is composed of two Spectral Instruments SI-1000 cameras located 20M from the target and provides neutron images of the DT hot spot for high yield shots. The development and commissioning of these new or enhanced diagnostics in FY11 have provided meaningful insight that facilitates the optimization of the four key Ignition variables. In FY12 they will be adding three new diagnostics and enhancing four existing diagnostics in support of the continuing optimization series of campaigns.

42 ENGINEERING↗

Final Report on the Plasma Liner Compression Experiment

The Field Reversed Configuration (FRC) plasmoid’s stability, robustness, and self-organization have been demonstrated in many experiments employing a variety of formation methods over the last 50 years. A new formation method capable of remotely forming a hot, stable, and stationary FRC that can be further compressed to fusion conditions has been developed at MSNW. The formation method consists of the super-sonic merging of two FRCs, which are independently formed outside of the central compression chamber. Upon collision in the center of the compression chamber, the FRCs merge and become stationary, thereby thermalizing their translational kinetic energy. In the Plasma Liner Compression (PLC) experiment, the target FRC is positioned in a compression chamber where it is either magnetically compressed by the increasing flux from a theta-pinch coil, or by the theta-pinch implosion of a pre-formed plasma liner. The plasma liner is formed via an annular array of 32 axially directed MPD plasma sources near the vacuum chamber walls in the compression area. Xenon gas is ionized by the plasma sources, magnetized to the ~ 0.1 T bias field which keeps the Xenon liner confined to an annulus near the chamber wall. The FRC is ideally vacuum isolated from the Xenon plasma liner due to the bias field, which also acts as the guide field required for FRC merging. Close agreement is found between experimental results and 2D MHD numerical calculations. Efficient coupling (~ 50%) between the compression bank and the plasma liner has been observed. Flux and plasma liner compression of merged FRCs were tested. While the flux compression of the merged FRC resulted in a hot fusion plasma, the liner interaction with the FRC at this scale cooled the plasma.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Layer of Protection Analysis for SIL Determination of the SCRAM System

The purpose of this calculation is to determine the Safety Integrity Level (SIL) required for the SCRAM System at the National Criticality Experiments Research Center (NCERC). The SCRAM System includes three Programmable Logic Controller (PLC) based subsystems. Each subsystem is designed to open a series of relays to initiate a SCRAM of the critical assembly machines. Each of these subsystems must be verified to meet their required Safety Integrity Level (SIL).

42 ENGINEERING↗

Tank Farm Safety Programmable System Software Management Plan

This Software Management Plan (SMP) applies to the configurable programmable logic controller (PLC) software configured as the Tank Farms Safety Programmable System (TFSPS). The TFSPS is identified as a safety-significant (SS) system to prevent loss of the safety function of SS structures, systems, and components (SSC). The TFSPS software is classified as grade level A configurable safety software. The TFSPS consists of safety instrumented systems (SIS) and safety instrumented alarm (SIA) systems.The purpose of this management plan is to describe how Washington River Protection Solutions (WRPS) will configure and manage the TFSPS software and the associated software life cycle (SLC). TFSPS software shall be configured by Process and Control Systems Engineering (P&CSE) as grade level A safety software for use by Production Operations as the primary stakeholders.

97 MATHEMATICS AND COMPUTING↗