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This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.
The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.
Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.
This data package contains phospholipid fatty acid and branched glycerol dialkyl glycerol tetraethers data used for the paper: “Whole-soil warming decreases abundance and modifies the community structure of microorganisms in the subsoil but not in surface soil”. SOIL, https://doi.org/10.5194/soil-2021-14.On April 2018 (after 4.5 years of warming), we collected soil cores down to 90 cm depth from the whole soil profile warming experiment at the University of California Blodgett Forest Research Station, in the Sierra Nevada foothills near Georgetown, CA at 1370 m above sea level. Cores were collected from 6 experimental plots (three replicated blocks); samples were collected in 10 cm increments from 0 to 90 cm depth.This data package includes a file in .csv format and a file in .xlsx format, which can be accessed and processed using MS Excel or R. Phospholipid fatty acid and branched glycerol dialkyl glycerol tetraethers concentrations are provided as processed data files.
This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation.
In this study, the Principal Investigator, F.R. Tabita has teamed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available “unrestrained” organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.
Microorganism viability in simulated space environments
Microorganisms and plants in a simulated martian environment
Effect of ultrahigh vacuum on viability of microorganisms
Detection and study of microorganisms in the upper atmosphere
Metabolic, ecologic, and genetic processes in terrestrial microorganisms under simulated planetary environments
Existence and identity of viable microorganisms in stratosphere
Continuous propagation of microorganisms - life cycle studies involving age distribution
Survival of terrestrial microorganism under simulated martian conditions
Survival of microorganisms in simulated environment of mars surface
Cold and aridity as constraints on survival of terrestrial microorganisms in simulated martian environment
Description of experimental requirements for adapting ultrahigh vacuum equipment to study of effects of simulated space environment on microorganisms
Microorganisms as indicators of solar activity and precursors of solar flares