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At least 145 records · Page 8

River Basin Export Reduction Optimization Support Tool; a tool to screen options for reducing nutrient loads while minimizing cost

Abstract Excess loading of nitrogen and phosphorus to river networks causes environmental harm, but reducing loads from large river basins is difficult and expensive. We developed a new tool, the River Basin Export Reduction Optimization Support Tool (RBEROST) to identify the least‐cost combinations of management practices that will reduce nutrient loading to target levels in downstream and mid‐network waterbodies. We demonstrate the utility of the tool in a case study in the Upper Connecticut River Basin in New England, USA. The total project cost of optimized lowest‐cost plans ranged from $18.0 million to $41.0 million per year over 15 years depending on user specifications. Plans include both point source and non‐point source management practices, and most costs are associated with urban stormwater practices. Adding a 2% margin of safety to loading targets improved the estimated probability of success from 37.5% to 99%. The large spatial scale of RBEROST, and the consideration of both point and non‐point source contributions of nutrients, make it well suited as an initial screening tool in watershed planning.

Chamberlin, Catherine↗

Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa

Filamentous fungi are intensively used for producing industrial enzymes, including lignocellulases. Employing insoluble cellulose to induce the production of lignocellulases causes some drawbacks, e.g., a complex fermentation operation, which can be overcome by using soluble inducers such as cellobiose. Here, a triple β-glucosidase mutant of Neurospora crassa, which prevents rapid turnover of cellobiose and thus allows the disaccharide to induce lignocellulases, was applied to profile the proteome responses to cellobiose and cellulose (Avicel). Our results revealed a shared proteomic response to cellobiose and Avicel, whose elements included lignocellulases and cellulolytic product transporters. While the cellulolytic proteins showed a correlated increase in protein and mRNA levels, only a moderate correlation was observed on a proteomic scale between protein and mRNA levels (R 2 = 0.31). Ribosome biogenesis and rRNA processing were significantly overrepresented in the protein set with increased protein but unchanged mRNA abundances in response to Avicel. Ribosome biogenesis, as well as protein processing and protein export, was also enriched in the protein set that showed increased abundance in response to cellobiose. NCU05895, a homolog of yeast CWH43, is potentially involved in transferring a glycosylphosphatidylinositol (GPI) anchor to nascent proteins. This protein showed increased abundance but no significant change in mRNA levels. Disruption of CWH43 resulted in a significant decrease in cellulase activities and secreted protein levels in cultures grown on Avicel, suggesting a positive regulatory role for CWH43 in cellulase production. The findings should have an impact on a systems engineering approach for strain improvement for the production of lignocellulases.

59 BASIC BIOLOGICAL SCIENCES↗

Bacteria export alarmone synthetases that produce (p)ppApp and (p)ppGpp

Guanosine penta- and tetraphosphate [(p)ppGpp] and their adenosine analogs [(p)ppApp] are bacterial second messengers known as alarmones. Members of the RelA-SpoT homolog (RSH) family synthesize (p)ppGpp to mediate the stringent response during nutrient starvation, whereas (p)ppApp synthetases have been identified as bactericidal toxins in diverse contexts including type VI secretion systems, toxin-antitoxin modules, and phages. Although alarmone synthesis has traditionally been viewed as a cytoplasmic process, early studies in Streptomyces suggested the existence of secreted alarmone synthetases. Here, we identify SaEAS, an exported alarmone synthetase (EAS) from Streptomyces albidoflavus, as the long-mysterious source of extracellular alarmone synthetase activity in Streptomyces. SaEAS produces both (p)ppGpp and (p)ppApp at rates exceeding 100,000 molecules per minute and has kinetic properties adapted to low substrate environments. A broader bioinformatic survey reveals ~600 EASs linked to a range of specialized bacterial secretion systems. Characterization of two additional EASs, VpEAS from Vibrio parahaemolyticus and AaEAS from Amycolatopsis azurea, shows that both produce (p)ppGpp exclusively and inhibit bacterial growth when localized to the cytoplasm. These findings challenge the longstanding view of (p)ppGpp as strictly pro-survival and unveil a diverse family of secreted RSH enzymes with potential roles in interbacterial antagonism and environmental signaling.

Ahmad, Shehryar↗

TILED-EXPORT

SF-25-094 A client for the Bluesky Tiled API that allows bulk export of files.

Wollfman, MarkF [Argonne National Laboratory (ANL)↗

Modeling the impact of riparian hollows on river corridor nitrogen exports, Frontiers in Water: Dataset

This package contains all reactive transport simulation outputs used in "Modeling the impact of riparian hollows on river corridor nitrogen exports" (Rogers, D.B., et al., 2021). The simulation outputs reflect hydrological and biogeochemical behavior of a single, generalized riparian hollow located in the intra-meander region of a sub-alpine floodplain under transient hydrological conditions. The context for, purpose of, and conclusions drawn from this model are detailed in Rogers, D.B., et al., 2021.The .zip file contains simulation outputs for each of the five time periods analyzed (see metadata for specific dates), with each folder representing one continuous simulation period. Within each folder are the raw simulation outputs, which are comprised of hundreds of files. Files ending in ".vtk" are best visualized and analyzed using ParaView (https://www.paraview.org). All other files are best viewed or analyzed using R. For a detailed description of the meaning of each file, refer to the ".fls" file located in each folder.

54 ENVIRONMENTAL SCIENCES↗

Data and scripts associated with “When do Riverine Systems 'Feel the Burn'? Simulating How Burn Extent and Severity Modulate Hydrologic Controls on Biogeochemical Export” (v2)

This data package is associated with the publication “When do Riverine Systems 'Feel the Burn'? Simulating How Burn Extent and Severity Modulate Hydrologic Controls on Biogeochemical Export” published in Water Resources Research (Wampler et al. 2025; preprint: https://doi.org/10.22541/essoar.174438106.63564767/v1). This study used the Soil and Water Assessment Tool (SWAT), a processed based model to explore the impacts of area burned and burn severity on streamflow, nitrate, and dissolved organic carbon (DOC) in two test basins: a semi-arid, mixed land use basin and a humid, primarily forested basin. We developed 1800 wildfire scenarios that we ran in each basin: 20 different burn extents (5 to 100% by 5%), 3 different burn severities (low, moderate, and high), and 30 different post-fire precipitation scenarios. We also ran an additional 30 scenarios associated with no wildfire for the 30 post-fire precipitation scenarios. For each scenario we were interested in the change in runoff ratio (streamflow) and average concentration and annual loads (nitrate and DOC) across the wildfire scenarios. This data package contains the data and scripts required to build SWAT models for the two test basins, create and run the wildfire scenarios, and generate the data summaries and figures used in the associated manuscript. This data package was originally published in March 2025. It was updated in January 2026 (v2; new and modified files) to include the final files after the manuscript went through reviews. See the change history section below for more details. For details on how to navigate data packages generated by this project, see https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About.

54 ENVIRONMENTAL SCIENCES↗

Export Control Guide: Loose Parts Monitoring Systems for Nuclear Power Plants

This report describes a typical LPMS, emphasizing its application to the RCS of a modern NPP. The report also examines the versatility of AE monitoring technology by describing several nuclear applications other than loose parts monitoring, as well as some non-nuclear applications. In addition, LPMS implementation requirements are outlined, and LPMS suppliers are identified. Finally, U.S. export controls applicable to LPMSs are discussed.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Unclassified Data Export from the Containment Database

The attached dataset is an unclassified subset of data copied from the CDB for release to a wider audience. It includes only the clearly unclassified columns from each of the tables in the CDB, and only the rows related to announced tests. Also included is a glossary that lists each of the tables and columns included in the export with its definition, historical labels, unit of measure, and reference information. Our objective is to make this unclassified dataset available for querying on an unclassified server through a repeatable process.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

On The Export Control Of High Speed Imaging For Nuclear Weapons Applications

Since the Manhattan Project, the use of high-speed photography, and its cousins flash radiography1 and schieleren photography have been a technological proliferation concern. Indeed, like the supercomputer, the development of high-speed photography as we now know it essentially grew out of the nuclear weapons program at Los Alamos2,3,4. Naturally, during the course of the last 75 years the technology associated with computers and cameras has been export controlled by the United States and others to prevent both proliferation among non-P5-nations and technological parity among potential adversaries among P5 nations. Here we revisit these issues as they relate to high-speed photographic technologies and make recommendations about how future restrictions, if any, should be guided.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Technical Basis for the International Export of Handheld Spectroscopy Detectors

The international export of handheld spectroscopy detectors by the National Nuclear Security Administration (NNSA) to partner states will provide state regulatory authorities and nuclear material owners a way to improve accountancy for accidental gains of nuclear material, including the provision of reports to the IAEA, in order to meet their safeguards agreements. International Atomic Energy Agency (IAEA) safeguards agreements for non-nuclear weapons states requires accountancy for all nuclear material. As defined in Article XX of the IAEA statute, nuclear material includes source materials: "uranium containing the mixture of isotopes occurring in nature," and special fissionable material: "plutonium-239; uranium-233; uranium enriched in the isotopes 235 or 233". For IAEA Member States to meet their requirements under comprehensive safeguards agreements (CSA), safeguards are to be applied on "all source or special fissionable material," which "includes all nuclear material subject to IAEA safeguards". Therefore, accidental gains and losses of nuclear material must be reported to the IAEA. An accidental gain occurs when a state unexpectedly adds nuclear material to their inventory by various means such as seizing smuggled material or the discovery of legacy items previously unaccounted for. The material type and quantity must be added to the State's inventory by updating domestic records and then communicated to the IAEA.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Export Administration Regulations (EAR) and Radiation Tolerant Microelectronics

Sandia National Laboratories was asked to provide a technical feasibility assessment on whether programmability is a viable means to address the radiation levels in the Export Administration Regulations (EAR). Most (if not all) modern Complementary Metal Oxide Semiconductor (CMOS) technologies will exceed at least one of the EAR radiation criteria inherently, there is no longer anything “special” about domestic parts or technologies that exceed EAR radiation criteria. A modern part today is not “designed” to exceed EAR criteria and in fact a part would need to be “specially designed” to fail to meet all of these criteria. And although it is possible to design a part to fail to meet the EAR radiation criteria in an unprogrammed state, such a part would be difficult to design in some technologies, may not achieve the ultimate radiation levels desired, would fail to meet core reliability principles, and may be unsuitable for high consequence applications.

07 ISOTOPE AND RADIATION SOURCES↗

Event-scale predictions of water and nitrogen exports in coastal watersheds

Focal Area(s): The purpose of this white paper is to call for the improvement of the predictability and explainability of the magnitude and timing of water and nutrient exports in coastal watersheds. In this regard, hybrid machine learning approaches and process-based numerical models provide new opportunities to maximize predictability, emulate process-based model components, and explicitly address model uncertainty associated with event-scale predictions in coastal watersheds.

54 ENVIRONMENTAL SCIENCES↗

Assessing the Export Potential for High Performance Materials

This document assesses the potential demand within international markets for HPMs in advanced ultra-supercritical (A-USC) and natural gas combined cycle (NGCC) power plants as well as the aerospace sector and estimates the potential economic impacts for the U.S. associated with the estimated HPM exports in each market.

36 MATERIALS SCIENCE↗

Considerations for Export Control of Accelerators and Accelerator Components

Although nuclear reactors have been the sole source for producing plutonium in all nuclear weapons states, accelerator-driven subcritical reactors are a tantalizing alternative. This report provides a review of accelerator driven technologies and components with a focus on export control.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Quantifying Variability and Controls of Riverine Dissolved Organic Carbon Exported to Arctic Coastal Margins of North America (Final Report)

This project involved implementation and application of a coupled permafrost hydrology and dissolved organic carbon process models to investigate how spatial and seasonal variations in terrestrial hydrology and soil freeze/thaw dynamics influence the mobilization, loading, and export of organic carbon to the stream network for selected arctic basins across northern Alaska and northwest Canada. The model simulations were constrained by detailed observations of in- stream chemistry, soil active layer profile moisture and temperature dynamics, streamflow, soil carbon inventories and satellite microwave remote sensing based assessments of surface soil freeze-thaw dynamics. We developed and applied the numerical modeling and data analysis, incorporating observed data for calibration and validation, in order to investigate the terrestrial hydrology, permafrost dynamics, and associated DOC production and loading to rivers across a region encompassing watersheds draining to the coast. The project produced six publications and three datasets archived in public repositories.

54 ENVIRONMENTAL SCIENCES↗

Geochemical exports to river from the intrameander hyporheic zone under transient hydrologic conditions, Water Resources Research: Dataset

To understand how redox processes influence carbon, nitrogen, and iron cycling within the intrameander hyporheic zone, we developed a biotic and abiotic reaction network and incorporated it into the reactive transport simulator PFLOTRAN. Two dimensional reactive flow and transport simulations were performed (1) to evaluate how transient hydrological conditions control the lateral redox zonation within an intrameander region of the East River in Colorado and (2) to quantify the impact of a single meander on subsurface exports of carbon and other geochemical species to the river. This data package includes simulated hydrologic and geochemical data from 23 Oct 2015 to 22 Oct 2016.

54 ENVIRONMENTAL SCIENCES↗

Modeling the Impact of Riparian Hollows on River Corridor Nitrogen Exports

Recent studies in snowmelt-dominated catchments have documented changes in nitrogen (N) retention over time, such as declines in watershed exports of N, though there is a limited understanding of the controlling processes driving these trends. Working in the mountainous headwater East River Colorado watershed, we explored the effects of riparian hollows as N-cycling hotspots and as important small-scale controls on observed watershed trends. Using a modeling-based approach informed by remote sensing and in situ observations, we simulated the N-retention capacity of riparian hollows with seasonal and yearly hydrobiogeochemical perturbations imposed as drivers. We then implemented a scaling approach to quantify the relative contribution of riparian hollows to the total river corridor N budget. We found that riparian hollows primarily serve as N sinks, with N-transformation rates significantly limited by periods of enhanced groundwater upwelling and promoted at the onset of rainfall events. Given these observed hydrologic controls, we expect that the nitrate ( NO 3 - ) sink capacity of riparian hollows will increase in magnitude with future climatic perturbations, specifically the shift to more frequent rainfall events and fewer snowmelt events, as projected for many mountainous headwater catchments. Our current estimates suggest that while riparian hollows provision ~5–20% of NO 3 - to the river network, they functionally act as inhibitors to upland NO 3 - reaching the stream. Our work linking transient hydrological conditions to numerical biogeochemical simulations is an important step in assessing N-retaining features relative to the watershed N budget and better understanding the role of small-scale features within watersheds.

54 ENVIRONMENTAL SCIENCES↗

Improved (ERTS) information and its impact on U.S. markets for agricultural commodities: A quantitiative economic investigation of production, distribution and net export effects

An econometric investigation into the markets for agricultural commodities is summarized. An overview of the effort including the objectives, scope, and architecture of the analysis and the estimation strategy employed is presented. The major empirical results and policy conclusions are set forth. These results and conclusions focus on the economic importance of improved crop forecasts, U.S. exports, and government policy operations. A number of promising avenues of further investigation are suggested.

Source record↗