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At least 145 records · Page 8

Uranium interaction with soil minerals in the presence of co-contaminants: Case Study- subsurface sediments at or below the water table

Uranium (U) contaminated subsurface systems are common on a global scale mainly because of its essential role in the production of plutonium for nuclear weapons and other nuclear energy and research activities. Studying the behavior and fate of U in these systems is challenging because of heterogeneities of different types (i.e., physical, chemical and mineralogical) and a complex network of often time-dependent hydrological, biological and chemical reactions and processes that occur sequentially or simultaneously, affecting and/or controlling U mobility. A U contaminated site, i.e., the Integrated Field Research Challenge site in Rifle, CO, USA (a former U mill site) is the focus of this discussion. The overall objectives of this chapter are to 1) provide an overview of the contamination levels (U and other co-contaminants) at this field site; 2) review and discuss different aspects of mineral-U contaminant interactions in reduced and oxidized environments, and in the presence of co-contaminants; 3) present results from a systematic macroscopic, microscopic, and spectroscopic study as an example of the current research efforts and the state-of-knowledge in this important research area; and 4) offer insightful conclusive remarks and future research needs about reactions and processes that control U and other contaminants’ fate and behavior under hydraulically saturated conditions. The implications and applications presented in this chapter are valid for U contaminated sites across the world.

As, V, Se contamination↗

An Acid-Free, Temperature-Based Cation Contamination Removal Strategy for PEM Water Electrolysis

It is widely understood that the durability and reliability of polymer electrolyte membrane (PEM) water electrolyzers are heavily dependent on feedwater purity, with cation contaminants that originate from incomplete water purification and balance of plant materials significantly harming electrolyzer performance. However, contamination remains a challenge and a common cause of failure at the stack level, indicating the need for strategies to recover the performance of contaminated cells. In this study, we investigate the effects of temperature on the uptake, electrochemical impacts, and removal of contaminant calcium and iron cations. Lower operating temperatures increase the sensitivity of the cell performance to contaminant cations, while also decreasing cation uptake and promoting contaminant removal. Computational charge transfer modelling shows that lower temperature increases the concentration of contaminant at the cathode and facilitates their removal from the cell. By testing single cells under scenarios designed to mimic stack temperature dynamics, we investigate low-temperature operation as an approach to stack-relevant contaminant recovery. Together, these results demonstrate that the low-temperature recovery approach is a promising approach for acid-free contamination recovery for PEM water electrolysis to promote stack reliability and durability.

08 HYDROGEN↗

Micro Raman Spectroscopy for Characterizing Molecular Contamination on Space Flight Hardware

In the field of contamination control,many unknowns exist. Often, contamination control engineers have to utilize multiple spectroscopic, macroscopic, and microscopic tools to identify contamination sources when there is a potential contamination exposure to space flight hardware. Understanding the chemical composition of the molecular contamination provides an opportunity for contamination control engineers to determine if this contamination is problematic or benign. In this study, an existing witness plate program for monitoring space flight hardware during the assembly, integration, and testing phases is coupled with micro Raman spectroscopy analysis. The main goal of this study is to determine the limitations, reproducibility, and throughput of micro Raman spectroscopy for characterizing molecular contamination. Micro Raman Spectroscopy will be an additional tool used by contamination control engineers to monitor the cleanliness of space flight hardware.

Microscope↗

Evaluation of Potential Dose to Off-site Receptors from On-site Removable Contamination - 18521

Calculations used in making corrective action decisions estimate the maximum potential dose to on-site receptors exposed to contamination from nuclear testing activities at U.S. Department of Energy, Environmental Management Nevada Program Soils Activity release sites. At many of these release sites, removable contamination is present at levels that require controls to be in place under Title 10 Code of Federal Regulations Part 835, 'Occupational Radiation Protection.' When significant levels of removable contamination are present at a release site, it may be necessary to evaluate the potential dose to off-site receptors from removable contamination that has the potential to be transported off the contaminated site. The protocols described herein could be used to evaluate the potential dose to off-site receptors to ensure that corrective action decisions consider both on-site and off-site potential receptors. The calculation of estimated dose to a receptor can be accomplished by establishing a scenario that describes the exposure (e.g., time and pathway) of the individual to the radiological contamination and by using the properties of the contaminants (e.g., dose conversion factors). Radiological contamination transported from the site has the potential to expose off-site receptors under a potentially large number of exposure scenarios that cannot be comprehensively defined. However, a scenario was developed to represent a maximally exposed off-site receptor. Because the estimated dose to the maximally exposed off-site receptor based on maximum post-closure release site activity levels is less than the on-site dose decision level, corrective action decisions at the Nevada National Security Site Soils Activity release sites are also protective of potential off-site receptors when significant levels of removable contamination are present. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Hydrogeologic Setting and Groundwater Contaminant Conceptual Model, Los Alamos National Laboratory - 18681

Our understanding of the complex hydrogeologic setting at Los Alamos National Laboratory (LANL) has evolved significantly over the previous decade. The semi-arid, high-desert setting consists of generally dry drainages in canyons that head either in uplands to the west of the Laboratory or on the high mountain-front plateau where the Laboratory is spread across approximately 40 square miles. Some canyon drainages have spatially limited reaches with perennial surface water from natural runoff and spring sources and/or from permitted effluent sources. The Rio Grande rift basin-fill stratigraphy includes primary volcanic deposits from pyroclastic eruptions and intermediate composition lava flows, and fluvial and alluvial fan deposits. Highly complex and spatially variable stratigraphy governs the occurrences of three groundwater zones: shallow 'alluvial groundwater', deep perched groundwater, and a thick 'regional' aquifer that is the water supply for the community of Los Alamos and the Laboratory. The current understanding of the hydrogeologic framework was greatly expanded as part of a site hydrogeologic characterization program completed in 2005. Higher resolution information exists in area where more detailed groundwater characterization has been conducted to support specific project objectives. A conceptual model for occurrences of contaminants in each of the three groundwater zones is governed by four factors: contaminant solubility/mobility, the mass of historical release, presence of a continuous hydraulic driver, and 'breakthrough' geology, defined as geology conducive to infiltration of shallow groundwater to deeper groundwater zones. The Laboratory's operations were historically somewhat spatially partitioned. Most of the radionuclide work (and historical releases) was conducted in the northern portion of the Laboratory, high explosives work has taken place exclusively in the southwestern portion, and treated sanitary effluent was released at a limited number of outfalls. Historical material disposal areas (MDAs) are dispersed throughout the Laboratory, and active MDAs are all located on a single dry mesa at the eastern part of the Laboratory. Evidence exists of transport of anthropogenic constituents to the regional aquifer. However, years of groundwater characterization have identified only two cases for which all the key factors were present and a complete contaminant pathway to the regional aquifer has resulted in contamination at levels above an applicable standard. Hexavalent chromium contamination associated with historical use as a corrosion inhibitor in a power plant is present beneath Mortandad Canyon, and RDX contamination associated with high-explosives machining is present beneath Canon de Valle. Each of those project areas have refined conceptual and numerical models that address contaminant migration pathways, spatial extent of perched-intermediate groundwater, mass balance, and fate and transport within the regional aquifer. No known contamination is present in the regional aquifer associated with MDAs, and interestingly, much of the Laboratory's groundwater background dataset is from wells located beneath active MDAs. A comprehensive groundwater monitoring program is in place to track the evolution of chromium and RDX contamination, and to identify any other constituents that may be attributable to historical LANL operations. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Amplicon Sequencing Assessment to Measure Microbial Community Response from Heavy Metal Contaminated Soils in Savannah River site, Tims Branch watershed

Long-term presence of heavy metal contamination in soils is correlated with changes in microbial community structures and can lead to the reduction of indigenous species. Tolerance to soil heavy metal toxicity varies among different microbial communities. It remains unclear whether long term effects caused by metal perturbations are associated with soil microbial community dysregulation in Tims Branch watershed. Research Questions: Does heavy metal contamination in Tims Branch soils decrease microbial species diversity significantly compared to non-contaminated soils? Does the species richness decrease in contaminated samples? between samples? Is diversity in low, mid and high contamination soils significantly different compared to control samples? Hypothesis: The relative abundance and diversity of species are significantly altered in soils which are contaminated with heavy metals in the Tims Branch watershed. Objectives: Use amplicon sequencing technology along with bioinformatics to compare bacterial communities between four sites in Tims Branch watershed. Evaluate the percent relative abundance and diversity across the site. Conclusions: All bacterial communities exposed to different heavy metal concentrations were dominated by four major groups (Proteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia). The dominant phyla Proteobacteria, Acidobacteria and Chloroflexi accounted for 61 % of the relative abundance. Soil bacterial α-diversity, expressed as observed species richness metric and Shannon's diversity index, was the highest in sample location four (high contamination). The lowest observed was at the control location. Species richness increased when concentrations of heavy metals were localized, inferring that the metals can alter microbial community structure. PERMANOVA analysis between contaminated groups tested significant using the Adonis (F (4,29) =1.9712; p < 0.001). There is a 20 percent variation explained between groups (R2 = 0.214). This analysis proves to be beneficial in detecting microbial communities altered by contaminated soils. Moreover, certain concentrations of heavy metals can alter microbial community structures.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Analysis of furnace contamination on superconducting radio frequency niobium using secondary-ion mass spectrometry

Detection of surface contamination on niobium materials used in superconducting radio frequency (SRF) applications is difficult due to quantitative sensitivity and near-atomic depth resolution needed. Inspection of samples known to have experienced surface contamination was found to have inconsistent nitride coverage after high-temperature nitrogen gas exposure (“doping”). Here we compare contaminating species found on samples treated in several different vacuum furnaces, both “exposed” directly in the chamber and “protected” by containment shielding from evaporative sources with “furnace caps.” Typically, furnace caps are used to impede contamination from reaching the interior surface of cavities during the high-temperature vacuum bake that immediately precedes exposure to nitrogen gas. Although, to date, little is known about the effectiveness of these caps, SIMS results showed that they were effective in limiting contamination arising from the furnace environment. Inspection of sample surfaces by SEM showed a lack of nitrides present on contaminated specimens. TEM with energy dispersive spectroscopy performed on these samples revealed that a carbon-rich layer now existed, indicating that a relatively high contaminant load prevents the nucleation and growth of surface nitrides, while thus inhibiting interstitial nitrogen uptake. Except in extreme cases, subsequent removal of the top several micrometers of the surface via electropolishing appears to effectively eliminate any strong influence on the subsequent SRF cavity performance. With the absence of furnace cleaning, carbon contamination was found to be nearly 10× higher for protected nitrogen-doped and electropolished samples, with minimal metallic contamination detected for both processes. SIMS analysis was also performed to compare the cleanliness of samples fully prepared by such nitrogen “doping” with those prepared by a related process, involving the dissolution of niobium surface oxide and diffusion of oxygen into the surface. This oxygen doping or alloying process offers attractive advantages.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Assessing Inhalation Exposures Associated with Contamination Events in Water Distribution Systems

When a water distribution system (WDS) is contaminated, short-term inhalation exposures to airborne contaminants could occur as the result of domestic water use. The most important domestic sources of such exposures are likely to be showering and the use of aerosol-producing humidifiers, i.e., ultrasonic and impeller (cool-mist) units. A framework is presented for assessing the potential effects of short-term, system-wide inhalation exposures that could result from such activities during a contamination event. This framework utilizes available statistical models for showering frequency and duration, available exposure models for showering and humidifier use, and experimental results on both aerosol generation and the volatilization of chemicals during showering. New models for the times when showering occurs are developed using time-use data for the United States. Given a lack of similar models for how humidifiers are used, or the information needed to develop them, an analysis of the sensitivity of results to assumptions concerning humidifier use is presented. The framework is applied using network models for three actual WDSs. Simple models are developed for estimating upper bounds on the potential effects of system-wide inhalation exposures associated with showering and humidifier use. From a system-wide, population perspective, showering could result in significant inhalation doses of volatile chemical contaminants, and humidifier use could result in significant inhalation doses of microbial contaminants during a contamination event. From a system-wide perspective, showering is unlikely to be associated with significant doses of microbial contaminants. In conclusion, given the potential importance of humidifiers as a source of airborne contaminants during a contamination event, an improved understanding of the nature of humidifier use is warranted.

54 ENVIRONMENTAL SCIENCES↗

Bacterial Community Dynamics in Dichloromethane-Contaminated Groundwater Undergoing Natural Attenuation

The uncontrolled release of the industrial solvent methylene chloride, also known as dichloromethane (DCM), has resulted in widespread groundwater contamination in the United States. Here we investigate the role of groundwater bacterial communities in the natural attenuation of DCM at an undisclosed manufacturing site in New Jersey. Here, we investigate the bacterial community structure of groundwater samples differentially contaminated with DCM to better understand the biodegradation potential of these autochthonous bacterial communities. Bacterial community analysis was completed using high-throughput sequencing of the 16S rRNA gene of groundwater samples (n = 26) with DCM contamination ranging from 0.89 to 9,800,000 μg/L. Significant DCM concentration-driven shifts in overall bacterial community structure were identified between samples, including an increase in the abundance of Firmicutes within the most contaminated samples. And across all samples, a total of 6,134 unique operational taxonomic units (OTUs) were identified, with 16 taxa having strong correlations with increased DCM concentration. Putative DCM degraders such as Pseudomonas, Dehalobacterium and Desulfovibrio were present within groundwater across all levels of DCM contamination. Interestingly, each of these taxa dominated specific DCM contamination ranges respectively. Potential DCM degrading lineages yet to be cited specifically as a DCM degrading organisms, such as the Desulfosporosinus, thrived within the most heavily contaminated groundwater samples. Co-occurrence network analysis revealed aerobic and anaerobic bacterial taxa with DCM-degrading potential were present at the study site. Our 16S rRNA gene survey serves as the first in situ bacterial community assessment of contaminated groundwater harboring DCM concentrations ranging over seven orders of magnitude. Diversity analyses revealed known as well as potentially novel DCM degrading taxa within defined DCM concentration ranges, indicating niche-specific responses of these autochthonous populations. Altogether, our findings suggest that monitored natural attenuation is an appropriate remediation strategy for DCM contamination, and that high-throughput sequencing technologies are a robust method for assessing the potential role of biodegrading bacterial assemblages in the apparent reduction of DCM concentrations in environmental scenarios.

16S rRNA↗

Characterization and Modeling of Contamination for Lyman Break Galaxy Samples at High Redshift

The selection of high-redshift sources from broadband photometry using the Lyman-break galaxy (LBG) technique is a well established methodology, but the characterization of its contamination for the faintest sources is still incomplete. We use the optical and near-IR data from four (ultra)deep Hubble Space Telescope legacy fields to investigate the contamination fraction of LBG samples at z∼5--8 selected using a color–color method. Our approach is based on characterizing the number count distribution of interloper sources, that is, galaxies with colors similar to those of LBGs, but showing detection at wavelengths shorter than the spectral break. Without sufficient sensitivity at bluer wavelengths, a subset of interlopers may not be properly classified, and contaminate the LBG selection. The surface density of interlopers in the sky gets steeper with increasing redshift of LBG selections. Since the intrinsic number of dropouts decreases significantly with increasing redshift, this implies increasing contamination from misclassified interlopers with increasing redshift, primarily by intermediate redshift sources with unremarkable properties (intermediate ages, lack of ongoing star formation and low/moderate dust content). Using Monte-Carlo simulations, we estimate that the CANDELS deep data have contamination induced by photometric scatter increasing from ∼2% at z∼5 to ∼6% at z∼8 for a typical dropout color ⩾1 mag, with contamination naturally decreasing for a more stringent dropout selection. Contaminants are expected to be located preferentially near the detection limit of surveys, ranging from 0.1 to 0.4 contaminants per arcmin{sup 2} at J{sub 125} = 30, depending on the field considered. This analysis suggests that the impact of contamination in future studies of z>10 galaxies needs to be carefully considered.

79 ASTRONOMY AND ASTROPHYSICS↗

Spacecraft contamination: Sources and prevention; Thermophysics Conference, 18th, Montreal, Canada, June 1-3, 1983, Selected Papers

Results of current research in the field of spacecraft contamination are presented. An overview of the various sources of contamination is given, showing the many and diverse mechanisms associated with the degradation of thermal and optical performance of spacecraft surfaces and optics. Some of the causes of contamination are discussed, including spallation of foam insulation on cryogenic fuel tanks, dispersion of particle contamination about a spacecraft, particle contamination of optical sensors, and the influence of the location of venting systems. Contamination prevention is addressed. Infrared transmittance and infrared optical properties of contaminants are considered along with the effects and properties of mixtures of exhaust species, of plume boundary layer flow, and of the contamination associated with bipropellant attitude control thrusters proposed for the Galileo spacecraft. Also discussed are: degradation of 'clean' thermal control surfaces over four years in orbit, in-orbit measurement of solar absorptance, and contamination from postfire efflux of a solid-propellant rocket motor.

Roux, J. A.↗

Spacecraft contamination programs within the Air Force Systems Command Laboratories

Spacecraft contamination programs exist in five independent AFSC organizations: Geophysics Laboratory (GL), Arnold Engineering and Development Center (AEDC), Rome Air Development Center (RADC/OSCE), Wright Research and Development Center (MLBT), Armament Laboratory (ATL/SAI), and Space Systems Division (SSD/OL-AW). In addition, a sizable program exists at Aerospace Corp. These programs are complementary, each effort addressing a specific area of expertise: GL's effort is aimed at addressing the effects of on-orbit contamination; AEDC's effort is aimed at ground simulation and measurement of optical contamination; RADC's effort addresses the accumulation, measurement, and removal of contamination on large optics; MLBT's effort is aimed at understanding the effect of contamination on materials; ATL's effort is aimed at understanding the effect of plume contamination on systems; SSD's effort is confined to the integration of some contamination experiments sponsored by SSD/CLT; and Aerospace Corp.'s effort is aimed at supporting the needs of the using System Program Offices (SPO) in specific areas, such as contamination during ground handling, ascent phase, laboratory measurements aimed at understanding on-orbit contamination, and mass loss and mass gain in on-orbit operations. These programs are described in some detail, with emphasis on GL's program.

Murad, Edmond↗

The Hubble Space Telescope (HST) Contamination Control Program

Over the past two decades, the Hubble Space Telescope (HST) Contamination Control Program has evolved from a ground-based integration program to a space-based science-sustaining program. On-orbit, the telescope s primary contamination requirement was maintaining a low contamination flux in the telescope s optical path. In addition, to maintain the scientific capability of the telescope, the contamination requirements and specific contamination controls from the second- and third-generation Scientific Instruments and Orbital Replacement Units were captured within the HST Contamination Control Program. Contamination controls were developed for on-orbit operations and four Servicing Missions (Orbiter, Astronauts, and mission). Long-term on-orbit scientific data has shown that these contamination controls successfully protected the HST from contamination.

Hansen, Patricia A.↗

Mir Contamination Observations and Implications to the International Space Station

A series of external contamination measurements were made on the Russian Mir Space Station. The Mir external contamination observations summarized in this paper were essential in assessing the system level impact of Russian Segment induced contamination on the International Space Station (ISS). Mir contamination observations include results from a series of flight experiments: CNES Comes-Aragatz, retrieved NASA camera bracket, Euro-Mir '95 ICA, retrieved NASA Trek blanket, Russian Astra-II, Mir Solar Array Return Experiment (SARE), etc. Results from these experiments were studied in detail to characterize Mir induced contamination. In conjunction with Mir contamination observations, Russian materials samples were tested for condensable outgassing rates in the U.S. These test results were essential in the characterization of Mir contamination sources. Once Mir contamination sources were identified and characterized, activities to assess the implications to ISS were implemented. As a result, modifications in Russian materials selection and/or usage were implemented to control contamination and mitigate risk to ISS.

Soares, Carlos↗

The Importance of Contamination Knowledge - Insights into Mars Sample Return

The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center (JSC), in Houston, TX (henceforth Curation Office) manages the curation of all past, present, and future extraterrestrial samples returned by NASA missions and shared collections from international partners, preserving their integrity for future scientific study while providing the samples to the international community in a fair and unbiased way. The Curation Office also curates all reference and witness materials for each mission (e.g., flight and non-flight hardware coupons; lubricants; non-flight, flight-like, and flown witness plates). These reference and witness materials provide the scientific community with the fundamental ability to reconstruct the contamination/alteration history of the sample collection through the course of the mission, with the overall goal of strengthening the scientific conclusions drawn from the study of returned materials. The information gained from characterizing the physical, biological, inorganic, and organic chemical properties of reference and witness materials is defined as the Contamination Knowledge (CK) of the sample collection. Unlike the data collected for Contamination Control (CC) and Planetary Protection (PP), CK is exclusively concerned with preserving reference and witness materials for study by future scientists upon sample return. Although CC and PP data collected for sample integrity and forward contamination purposes can be complementary to CK, they are two separate data sets with distinct objectives. A robust collection of samples for CK is necessary to allow the extraterrestrial material in a returned sample to be distinguished from terrestrial contamination. Traditionally CK is utilized by sample scientists in order to accomplish the mission’s scientific objectives, however this information can also be utilized by the Office of Planetary Protection to help evaluate the presence of any back contamination. Mars 2020, the first phase of a potential multipart Mars Sample Return (MSR) campaign, is expected to contribute to NASA’s Mars Exploration Program Science Goals by filling in knowledge gaps concerning: 1) the existence of past or present life on Mars, 2) the past and present climate of Mars, 3) the geology of Mars, and 4) hazards associated with human exploration of Mars. Although there is debate concerning which samples will best answer these questions, the necessity for proper sample blanks is well-understood. The CC and PP requirements, driven by the restricted Class V mission designation, are the most stringent of any sample return mission in recent history. The extremely low levels of allowable terrestrial contamination on the spacecraft and rover can complicate these analyses given the detection limits of current analytical instrumentation, especially in the case of biological contamination. By collecting and curating unanalyzed samples specifically for CK, future sample scientists will not be relegated to: 1) relying on data collected using possibly obsolete tools and techniques for return sample blanks, or 2) using remnants of extracted and/or cultured samples from ATLO (Assembly, Test, and Launch Operations), which could be incompatible with the desired experimental endpoints or state-of-the-art techniques available at the time of sample return.The addition of biological experimental endpoints to a sample return campaign’s objectives broadens the requisite range in preservation environments (e.g. inert ultra-pure nitrogen gaseous environment at 18 degrees Centigrade versus less than or equal to minus 80 degrees Centigrade) and types of CK samples. As a result, the Curation Office will also curate the following CK samples at less than or equal to minus 80 degrees Centigrade for the Mars 2020 mission: 1) unanalyzed swabs and wipes in sterile containers, 2) all recirculation filters from the clean rooms used for sample and caching subsystem assembly and all filters from the laminar flow benches used to assemble sample intimate hardware, and 3) witness plates collecting airborne contamination within the assembly clean rooms. It has been Curation Office policy since the Apollo missions to preserve as many pristine samples as possible for future scientific research. Although CK is required to be collected for all stages of the MSR campaign, the CK for the Mars 2020 mission is the most critical for understanding contamination in the returned samples given the intimacy between the Martian samples and the Mars 2020 flight hardware. This presentation highlights the importance of CK for sample return missions as well as the traditional and novel types of CK samples required for a successful MSR campaign.

Harrington, A. D.↗

Blind to Chemistry: Molecular Contaminant Films We Could Be Missing During Visual Inspections and the Potential Impact to System Performance

Throughout the assembly, integration and test process, molecular contamination levels of space mission hardware are monitored to meet system performance requirements. Qualitatively, reflective surfaces and witness mirrors are continuously inspected for the visible presence of molecular contaminant films. Quantitatively, periodic reflectance measurements of witness mirrors indicate changes of mirror reflectivity over time due to the accumulation of molecular contaminant films. However, both methods only consider the presence of a contaminant film and not the molecular composition. Additionally, there is a risk that hardware may appear to be “visibly clean” even with a molecular contaminant film present on critical surfaces. To address these issues, experiments were performed to quantify the maximum molecular contaminant film that could be missed in visual inspections on witness mirrors with five different contaminants present. The corresponding changes in mirror reflectivity were modeled using the program STACK to determine the impact to space mission hardware performance. The results of this study not only show the criticality in considering the chemical make-up of molecular contaminant films on system performance, but also the need to recognize and understand the limitations of traditional visual inspection techniques on detecting molecular contaminant films.

Seasly, Elaine↗

A METHOD TO REDUCE BIOBURDEN IN ASTROMATERIALS CURATION FACILITIES WITHOUT INTRODUCING UNWANTED CONTAMINATION

Introduction: NASA curates its Astromaterials collections in cleanrooms that are carefully monitored for particulate, inorganic and trace metal contamination. Current sample collections are not particularly susceptible to organic contamination or biological alteration. However, new collections like those from the OSIRIS-REx and Hayabusa2 missions will have organic contamination requirements and are susceptible to biodegradation. It will be necessary sterilize or at least disinfect curation labs, as well as tools and equipment in a manner that does not introduce additional contamination and does not affect the samples 1. Current curation cleaning procedures utilize isopropyl alcohol which offers some bioburden reduction, but is not effective against spore-forming bacteria or fungal spores 2. We present a modified disinfection method that uses ultrapure hydrogen peroxide to reduce bioburden inside curation labs and glove boxes without introducing contamination or damaging curation equipment. We tested this method in the meteorite processing lab as well as on a glovebox being cleaned for use in processing ANGSA (Apollo Next Generation Sample Analysis) samples and present the results of those tests. We discuss the limitations of this method and describe potential situations in which it will not be applicable. The CDC guidelines for disinfection andsterilization in healthcare facilities discusses over 15different methods for reducing bioburden in hospitalsettings 3. The most common method, steamsterilization, is well suited to sterilizing curationprocessing tools but cannot easily be used to sterilizecleanroom surfaces or large equipment likegloveboxes. Chemical sterilization with bleach(NaOCl) is also a common strategy in healthcare andpharmaceutical settings that presents materialcompatibility issues as well as serious inorganiccontamination concerns for curation facilities.Introducing a new source of Na and Cl into curationlabs is not acceptable. Other chemical methods likeethylene oxide, formaldehyde, iodophors andquaternary ammonium compounds could introduceorganic and inorganic contamination. We chose tofocus on hydrogen peroxide because it is generallycompatible with commonly used curation materialslike stainless steel, aluminum and Teflon and becauseit decomposes to oxygen and water. The CDCguidelines for hydrogen peroxide specify using a 7.5wt% solution at 25 ̊C with a contact time of 30 minutesfor high level disinfection and 6 hours for sterilization.High level disinfection is defined as a technique thatwill kill all microorganisms except large numbers ofbacterial spores 3. Methods: We prepared a solution of 7.5 wt%hydrogen peroxide from a stock solution of ultrapure30 wt% peroxide (JT Baker) and curation gradeultrapure water. This ultrapure water is already used incuration cleaning procedures and thus is not consideredand additional source of contamination. We conducteda materials compatibility test by exposing unanodizedand anodized 6061 T6 Al alloy to the peroxide solutionfor up to six hours and periodically inspecting thesurfaces for visible defects. We used this peroxide todisinfect the floor of the meteorite processing lab andthe interior of a curation glovebox by exposing thesesurfaces to the peroxide solution for 30 min. Thesurfaces were swabbed with a dry macrofoam swabbefore (Puritan Brand 2518051PFRNDFD) and afterperoxide treatment to collect microbes present on thesurfaces. Microbes were extracted by sonication fromthe swab into 15 ml of PBS (phosphate buffered saline)and inoculated onto the following media: TSA (trypticsoy agar) BA (blood agar), R2A (Reasoners 2 agar),Potato Dextrose Agar, Saboraud Dextrose Agar andSaboraud Dextrose Agar with 0.1 mg/ mlchloramphenicol. Four TSA plates and two BA plateswere inoculated with 0.1 ml of PBS each andincubated at 35 and 37 for 48 hours. Two R2A°C°Cplates (0.1 ml of PBS each) were incubated at 25 .°CThe remaining plates were inoculated with 0.2ml ofPBS and incubated at 30 ̊C for seven days. Afterincubation bacterial and fungal isolates were countedand transferred to new plates for identification usingthe VITEK24 automated system or by sequencing aportion of the barcode gene (16S rRNA for bacteria,small subunit gene for fungi) on an ABI 3500 Sangersequencer. Negative controls consisted of swabs thatwere opened in the sampling environment andanalyzed alongside the experimental samples.Results: A 6 hour exposure to hydrogen peroxideresulted in visible pitting on un-anodized 6061 Al, butnot on anodized surfaces. No visible pitting occurredafter a 30 minute exposure. Therefore, we decided tolimit our experimental tests to 30 min. exposures. 17bacterial CFU (colony forming units) representing 4distinct organisms were isolated from the meteorite processing lab floor prior to hydrogen peroxidetreatment. We were unable culture any organisms afterperoxide treatment. In the glovebox we were able toculture three bacterial CFU representing three distinctspecies, including a spore forming bacterium prior todisinfection with peroxide. After the peroxidetreatment we were unable to culture any organisms.Routine monitoring of the meteorite processing lab andthe glovebox did not indicate any increase in unwantedinorganic contamination after these peroxidetreatments. Discussion: A 30 minute treatment with 7.5 wt%peroxide appears to be an effective method forreducing bioburden on typical cleanroom surfaces. Themethod does not introduce unwanted organic orinorganic contamination and is compatible withcommonly used curation materials like stainless steel,Teflon and anodized aluminum alloys. Special careshould be taken with un-anodized aluminum.Prolonged exposure to hydrogen peroxide can causepitting on this material. We recommend using thismethod to disinfect curation labs and equipment whenbiological alteration is a concern. This method iseffective at room temperature and cannot be used todisinfect labs and equipment where the ambienttemperature is < 0 ̊C. Astromaterials samples shouldbe removed from the area where disinfection is tooccur. Hydrogen peroxide is a powerful oxidizingagent and will react with any organic carbon present inthe sample. References: [1.] Mccubbin, F. M. et al.Sp. Sci Rev(2019) doi:10.1007/s11214-019-0615-9. [2.] Mogul, R.et al.Astrobiology 18, ast.2017.1814 (2018). [3.]Rutala, W. A. & Weber, D. J. Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008. [4.] Pincus, D. H. in Encyclopedia of Rapid Microbiological Methods (2005).

A. B. Regberg↗

Machine learning models for estimating contamination across different curbside collection strategies

Contaminated recyclables, which are frequently discarded as waste, pose a significant challenge to the implementation of a circular economy. These contaminated recyclables impede the circulation of resources, resulting in higher processing costs at material recovery facilities (MRFs). Over the past few decades, machine learning (ML) models such as linear regression (LR), support vector machine (SVM), and random forest (RF) have evolved to provide new methods for predicting inbound contamination rates in addition to traditional statistical models. In this study, we applied ML models to predict inbound contamination rates using demographic features from 15 counties in the U.S. with different curbside collection strategies. In general, we found that ML models outperformed linear mixed models. Specifically, SVM models had the highest performance (R 2 = 0.75; mean absolute error (MAE) = 0.06), which may be due to their ability to model nonlinear relationships between features and inbound contamination rates. Further, the key predictor was population, with poverty rate being positively correlated and median age negatively correlated with inbound contamination rates. To improve the management of contamination and enhance the implementation of a circular economy, better models are needed to understand and estimate inbound contamination rates as well as identify critical factors in the present and future.

54 ENVIRONMENTAL SCIENCES↗