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At least 1,423 records · Page 79

Terrestrial Subsurface Ecosystem

The Earth’s crust is a solid cool layer that overlays the mantle, with a varying thickness of between 30-50 km on continental plates, and 5-10 km on oceanic plates. Continental crust is composed of a variety of igneous, metamorphic, and sedimentary rocks that weather and re-form over geologic cycles lasting millions to billions of years. At the crust surface, these weathered minerals and organic material combine to produce a variety of soils types that provide suitable habitats and niches for abundant microbial diversity (see Chapter 4). Beneath this soil zone is the subsurface. Once thought to be relatively free of microorganisms, recent estimates have calculated that between 1016-1017 g C biomass (2-19% of Earth’s total biomass) may be present in this environment (Whitman et al., 1998;McMahon and Parnell, 2014). Microbial life in the subsurface exists across a wide range of habitats: in pores associated with relatively shallow unconsolidated aquifer sediments to fractures in bedrock formations that are more than a kilometer deep, where extreme lithostatic pressures and temperatures are encountered. While these different environments contain varying physical and chemical conditions, the absence of light is a constant. Despite this, diverse physiologies and metabolisms enable microorganisms to harness energy and carbon for growth in water-filled pore spaces and fractures. Carbon and other element cycles are driven by microbial activity, which has implications for both natural processes and human activities in the subsurface, e.g., bacteria play key roles in both hydrocarbon formation and degradation. Hydrocarbons are a major focus for human utilization of the subsurface, via oil and gas extraction and potential geologic CO2 sequestration. The subsurface is also utilized or being considered for sequestered storage of high-level radioactive waste from nuclear power generation and residual waste from past production of weapons grade nuclear materials. While our understanding of the subsurface is continually improving, it is clear that only a small fraction of microbial habitats have been sampled and studied. In this chapter, we will discuss these studies in the context of the distribution of microbial life in the subsurface, the stresses that microorganisms must overcome to survive in these environments, and the metabolic strategies that are employed to harness energy in a region of the planet far-removed from sunlight. Finally, we will consider both beneficial and deleterious effects of microbial activity in the subsurface on human activities in this environment.

Wilkins, Michael J.↗

Can the Results of Biodiversity-Ecosystem Productivity Studies Be Translated to Bioenergy Production?

Biodiversity experiments show that increases in plant diversity can lead to greater biomass production, and some researchers suggest that high diversity plantings should be used for bioenergy production. However, many methods used in past biodiversity experiments are impractical for bioenergy plantings. For example, biodiversity experiments often use intensive management such as hand weeding to maintain low diversity plantings and exclude unplanted species, but this would not be done for bioenergy plantings. Also, biodiversity experiments generally use high seeding densities that would be too expensive for bioenergy plantings. Here we report the effects of biodiversity on biomass production from two studies of more realistic bioenergy crop plantings in southern Michigan, USA. One study involved comparing production between switchgrass (Panicum virgatum) monocultures and species-rich prairie plantings on private farm fields that were managed similarly to bioenergy plantings. The other study was an experiment where switchgrass was planted in monoculture and in combination with increasingly species-rich native prairie mixtures. Overall, we found that bioenergy plantings with higher species richness did not produce more biomass than switchgrass monocultures. The lack of a positive relationship between planted species richness and production in our studies may be due to several factors. Non-planted species (weeds) were not removed from our studies and these non-planted species may have competed with planted species and also prevented realized species richness from equaling planted species richness. Also, we found that low seeding density of individual species limited the biomass production of these individual species. Finally, production in future bioenergy plantings with high species richness may be increased by using a high density of inexpensive seed from switchgrass and other highly productive species, and future efforts to translate the results of biodiversity experiments to bioenergy plantings should consider the role of seeding density.

59 BASIC BIOLOGICAL SCIENCES↗

Higher Trophic Levels Overwhelm Climate Change Impacts on Terrestrial Ecosystem Functioning

Forest floor food webs play pivotal roles in carbon cycling, but they are rarely considered in models of carbon fluxes, including soil carbon dioxide emissions (respiration), under climatic warming. The indirect effects of invertebrates on heterotrophic (microbial and invertebrate) respiration through interactions with microbial communities are significant and will be altered by warming. However, the interactive effects of invertebrates and warming on heterotrophic respiration in the field are poorly understood. In this study we combined field and common garden laboratory approaches to examine relationships between warming, forest floor food web structure, and heterotrophic respiration. We found that soil animals can overwhelm the effects of warming (to 5 degrees Celsius above ambient) on heterotrophic respiration. In particular, the presence of higher trophic levels and burrowing detritivores strongly determined heterotrophic respiration rates in temperate forest soils. These effects were, however, context-dependent, with greater effects in a lower-latitude site. Without isolating and including the significant impact of invertebrates, climate models will be incomplete, hindering well-informed policy decisions.

59 BASIC BIOLOGICAL SCIENCES↗

The potential impact of new Andean dams on Amazon fluvial ecosystems

Increased energy demand has led to plans for building many new dams in the western Amazon, mostly in the Andean region. Historical data and mechanistic scenarios are used to examine potential impacts above and below six of the largest dams planned for the region, including reductions in downstream sediment and nutrient supplies, changes in downstream flood pulse, changes in upstream and downstream fish yields, reservoir siltation, greenhouse gas emissions and mercury contamination. Together, these six dams are predicted to reduce the supply of sediments, phosphorus and nitrogen from the Andean region by 69, 67 and 57% and to the entire Amazon basin by 64, 51 and 23%, respectively. These large reductions in sediment and nutrient supplies will have major impacts on channel geomorphology, floodplain fertility and aquatic productivity. These effects will be greatest near the dams and extend to the lowland floodplains. Attenuation of the downstream flood pulse is expected to alter the survival, phenology and growth of floodplain vegetation and reduce fish yields below the dams. Reservoir filling times due to siltation are predicted to vary from 106–6240 years, affecting the storage performance of some dams. Total CO2 equivalent carbon emission from 4 Andean dams was expected to average 10 Tg y-1 during the first 30 years of operation, resulting in a MegaWatt weighted Carbon Emission Factor of 0.139 tons C MWhr-1. Mercury contamination in fish and local human populations is expected to increase both above and below the dams creating significant health risks. Reservoir fish yields will compensate some downstream losses, but increased mercury contamination could offset these benefits.

54 ENVIRONMENTAL SCIENCES↗

The Alliance of Genome Resources: Building a Modern Data Ecosystem for Model Organism Databases

Model organisms are essential experimental platforms for discovering gene functions, defining protein and genetic networks, uncovering functional consequences of human genome variation, and for modeling human disease. For decades, researchers who use model organisms have relied on Model Organism Databases (MODs) and the Gene Ontology Consortium (GOC) for expertly curated annotations, and for access to integrated genomic and biological information obtained from the scientific literature and public data archives. Through the development and enforcement of data and semantic standards, these genome resources provide rapid access to the collected knowledge of model organisms in human readable and computation-ready formats that would otherwise require countless hours for individual researchers to assemble on their own. Since their inception, the MODs for the predominant biomedical model organisms [Mus sp (laboratory mouse), Saccharomyces cerevisiae, Drosophila melanogaster, Caenorhabditis elegans, Danio rerio, and Rattus norvegicus] along with the GOC have operated as a network of independent, highly collaborative genome resources. In 2016, these six MODs and the GOC joined forces as the Alliance of Genome Resources (the Alliance). By implementing shared programmatic access methods and data-specific web pages with a unified "look and feel," the Alliance is tackling barriers that have limited the ability of researchers to easily compare common data types and annotations across model organisms. To adapt to the rapidly changing landscape for evaluating and funding core data resources, the Alliance is building a modern, extensible, and operationally efficient "knowledge commons" for model organisms using shared, modular infrastructure.

59 BASIC BIOLOGICAL SCIENCES↗

Mycorrhiza better predict soil fungal community composition and function than aboveground traits in temperate forest ecosystems

We sampled soil in the organic and mineral horizons beneath two AM-associated (Fraxinus americana, Thuja occidentalis) and two ECM-associated tree species (Betula alleghaniensis, and Tsuga canadensis), with an evergreen and deciduous species in each mycorrhizal group. To characterize fungal communities and organic matter decomposition beneath each tree species, we sequenced the ITS1 region of fungal DNA and measured the potential activity of carbon and nitrogen-targeting extracellular enzymes. Each tree species harbored distinct fungal communities, supporting the need to consider both mycorrhizal type and leaf habit. However, between tree characteristics, mycorrhizal type better predicted fungal communities. Across fungal guilds, saprotrophic fungi were the most important group in shaping fungal community differences in soils beneath all tree species. The effect of leaf habit on carbon and nitrogen-targeting hydrolytic enzymes depended on tree mycorrhizal association in the organic horizon, while oxidative enzyme activities were higher beneath EcM-associated trees across both soil horizons and leaf habits. These data include extracellular hydrolytic and oxidative enzyme activities, ITS sequencing fungal community data, soil carbon to nitrogen, and soil pH. Site level data include climate (mean annual temperature, precipitation), elevation, and soil series and order information.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Whole Ecosystem Warming (WEW) Environmental Data and Water Table Summaries, Marcell Experimental Forest, Minnesota, 2015-2024

This data set contains observations of photosynthetically active radiation (PAR), precipitation, soil temperature, soil volumetric water content, air temperature, relative humidity, and normalized water table depth that are summarized on a daily, weekly, monthly, and annual basis for each of the SPRUCE plots. Observations span 2015-2024. This dataset draws on several datasets (Hanson et al. 2016; Hanson et al. 2020; and Warren, unpublished data) and compiles these environmental observations into useful formats for data analysis. These environmental metrics can be used to understand the environmental conditions inside SPRUCE environmental chambers throughout the durations of the experiment and can be paired with other data for modeling and analysis. R code used to generate these files is provided as part of the data package. This dataset contains four data files in comma separate (.csv) format and a compressed folder (*.zip) containing three R (*.r) scripts. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format. User note: Users must cite the original dataset/s along with this dataset when publishing any analyses using this dataset. Details on the dataset used to compile each variable are available in the header row of the files and in the user guide.

air temperature↗

Humic Substances Extracted by Alkali Are Invalid Proxies for the Dynamics and Functions of Organic Matter in Terrestrial and Aquatic Ecosystems

The conceptual rigor of using organic materials extracted from soil by alkali, called humic substances, as proxies for soil organic matter has been questioned for almost 180 years. However, the humic substances paradigm, i.e., the understanding that alkali-extracted humic substances are newly synthesized materials with unique properties that are distinct from non-humic organic matter, continues to be propagated throughout the literature. Here, we revisit the mechanistic background of the extraction process to show that alkaline extraction is unable to discriminate for the chemical history of a compound. For this reason, alkaline extraction cannot distinguish between (i) materials that have undergone secondary synthesis or humification (“humic” substances), and (ii) materials that are decorated with ionizable functional groups for other reasons, such as oxidative depolymerization (“non-humic” substances). While this mechanistic consideration alone renders invalid a distinction between humic substances and non-humic substances based on alkaline extraction, we further show that the evidence available to date does not support the assumption that processes of secondary synthesis create quantitatively significant proportions of “chemically reactive, yet recalcitrant” materials in natural environments. Any definition of humic substances that invokes both alkaline extraction and secondary synthesis is thus flawed on at least two accounts: (i) alkaline extraction is unable to achieve its purpose of separating humic from non-humic substances, and (ii) the assertion that the extracted materials have unique molecular properties as a consequence of secondary synthesis cannot be proven because alkaline extraction cannot separate materials created by secondary synthesis from other, ionizable organic compounds. Finally, we point out that since the definition of humic substances is tied to the alkaline-extraction procedure, neither the existence of operationally defined humic substances in the environment nor their chemical integrity during the course of alkaline extraction can be independently verified. We conclude that organic materials extracted by alkali require appropriate nomenclature and rigorous definition to merit consideration in teaching, research, and application.

54 ENVIRONMENTAL SCIENCES↗

Fire Impacts on the Mojave Desert Ecosystem: Literature Review

The Nevada National Security Site (NNSS) is located within the Mojave Desert, which is the driest region in North America. Precipitation on the NNSS varies from an annual average of 130 millimeters (mm; 5.1 inches) with a minimum of 47 mm (1.9 inches) and maximum of 328 mm (12.9 inches) over the past 15 year period to an annual average of 205 mm (8.1 inches) with an annual minimum of 89 mm (3.5 inches) and maximum of 391 mm (15.4 inches) for the same time period; for a Frenchman Flat location at 970 meters (m; 3182 feet) and a Pahute Mesa location at 1986 m (6516 feet), respectively. The combination of aridity and temperature extremes has resulted in sparsely vegetated basins (desert shrub plant communities) to moderately vegetated mountains (mixed coniferous forest plant communities); both plant density and precipitation increase with increasing elevation. Whereas some plant communities have evolved under fire regimes and are dependent upon fire for seed germination, plant communities within the Mojave Desert are not dependent on a fire regime and therefore are highly impacted by fire (Brown and Minnich, 1986; Brooks, 1999). As noted by Johansen (2003) natural range fires are not prevalent in the Mojave and Sonoran Deserts because there is not enough vegetation present (too many shrub interspaces) to sustain a fire. Fire research and hence publications addressing fires in the Southwestern United States (U.S.) have therefore focused on forest, shrub-steppe and grassland fires caused by both natural and anthropogenic ignition sources. In the last few decades, however, invasion of mid-elevation shrublands by non-native Bromus madritensis ssp. rubens and Bromus tectorum (Hunter, 1991) have been highly correlated with increased fire frequency (Brooks and Berry, 2006; Brooks and Matchett, 2006). Coupled with the impact of climate change, which has already been shown to be playing a role in increased forest fires (Westerling et al., 2006), it is likely that the fire frequency will further increase in the Mojave Desert (Knapp 1998; Smith et al., 1987; Smith et al., 2000).

(1.9↗

GAS-HYBRID SOURCE 14C-AMS: Application to terrestrial ecosystem, biological, and environmental research

Radiocarbon ( 14 C), a rare isotope of carbon (1:1012) which undergoes radioactive decay, is used to answer fundamental and applied questions of interest to DOE's Office of Science Biological and Environmental Research and Basic Energy Science research portfolio. Typically analyzed by accelerator mass spectrometry (AMS), radiocarbon measurements provides the ability to determine biogeochemical process rates and due to its unique tracer applicability to identify sources and pathways of carbon at the molecular to landscape scale.

54 ENVIRONMENTAL SCIENCES↗

A Collaborative Proposal: Simulating and Understanding Abrupt Climate-Ecosystem Changes During Holocene with NCAR-CCSM3

We have made significant progress in our proposed work in the last 4 years (3 years plus 1 year of no cost extension). In anticipation of the next phase of study, we have spent time on the abrupt changes since the last glacial maximum. First, we have performed further model-data comparison based on our baseline TRACE-21 simulation and made important progress towards the understanding of several major climate transitions. Second, we have made a significant effort in processing the model output of TRACE-21 and have put this output on a website for access by the community. Third, we have completed many additional sensitivity experiments. In addition, we have organized synthesis workshops to facilitate and promote transient model-data comparison for the international community. Finally, we have identified new areas of interest for Holocene climate changes.

54 ENVIRONMENTAL SCIENCES↗

Atmosphere, Climate and Ecosystem Sciences Team Summary

We engage in experimental and observational research and operations, with the goal of improving theory and models of climate change impacts and feedbacks for human and natural systems. We have a strong focus on understanding and quantifying carbon and water cycles, vegetation dynamics, aerosol-cloud interactions, and landscapes.

54 ENVIRONMENTAL SCIENCES↗

Genes, isotopes, and ecosystem biogeochemistry. Dissecting methane flux at the leading edge of global change

This project integrates across three fields (microbiology, biogeochemistry, and modeling) to understand the mechanisms of methane cycling in thawing permafrost. We have made substantial progress in each area, and in cross-cutting interdisciplinary synthesis. Large releases of CH 4 from thawing permafrost to the atmosphere, a strong positive feedback to global warming, are plausible but little is known about the controls on such release. Our project (“IsoGenie”) addresses the key question: What is the interplay of microbial communities and soil organic matter composition in the decomposition of organic C to CH 4 across a permafrost thaw gradient?

54 ENVIRONMENTAL SCIENCES↗