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A technique has been developed to refine the unit-cell parameters of single crystals with minimization of the influence of instrumental errors on the result. The corresponding computational procedure HuberUB is added to the software package of Huber-5042 diffractometer with a point detector and closedcycle helium cryostat Displex DE-202. The parameters of unit cell, its orientation, the goniometer zero angles, the sample eccentricity, the distances in the goniometer, and the radiation wavelength were refined by the nonlinear least-squares method, which allows imposition of constraints on the unit-cell parameters, depending on the crystal symmetry. The technique is approved on a LuB{sub 12} single crystal. The unit-cell parameters are determined in a temperature range of 20–295 K, with an absolute error not larger than 0.0004 Å (the relative error is of ~5 × 10{sup –5}). The estimates of the unit-cell parameters obtained by the proposed method are evidenced to be unbiased. Some specific features of the behavior of parameters in the ranges of 120–140 and 20–50 K are revealed, which correlate with the anomalies of the physical properties of the crystal.
The leaf economics spectrum (LES) describes multivariate correlations in leaf structural, physiological and chemical traits, originally based on diverse C3 species grown under natural ecosystems. However, the specific contribution of C4 species to the global LES is studied less widely. C4 species have a CO2 concentrating mechanism which drives high rates of photosynthesis and improves resource use efficiency, thus potentially pushing them towards the edge of the LES. Here, we measured foliage morphology, structure, photosynthesis, and nutrient content for hundreds of genotypes of the C4 grass Miscanthus × giganteus grown in two common gardens over two seasons. We show substantial trait variations across M. × giganteus genotypes and robust genotypic trait relationships. Compared to the global LES, M. × giganteus genotypes had higher photosynthetic rates, lower stomatal conductance, and less nitrogen content, indicating greater water and photosynthetic nitrogen use efficiency in the C4 species. Additionally, tetraploid genotypes produced thicker leaves with greater leaf mass per area and lower leaf density than triploid genotypes. By expanding the LES relationships across C3 species to include C4 crops, these findings highlight that M. × giganteus occupies the boundary of the global LES and suggest the potential for ploidy to alter LES traits.
We report on the fabrication and characterization of nanocrystalline ZnO films for use as a random laser physical unclonable function (PUF). Correlation between processing conditions and film microstructure will be made to optimize the lasing properties and random response. We will specifically examine the repeatability and security of PUFs demonstrated in this novel system. This demonstration has promise to impact many of Sandia's core missions including counterfeit detection.
In this study, we explore the similarities and differences between variational Monte Carlo techniques that employ conventional and artificial neural network representations of the ground-state wave function for fermionic systems. Our primary focus is on shallow neural network architectures, specifically the restricted Boltzmann machine, and we examine unsupervised learning algorithms that are appropriate for modeling complex many-body correlations. We assess the advantages and drawbacks of conventional and neural network wave functions by applying them to a range of circular quantum dot systems. Our findings, which include results for systems containing up to 90 electrons, emphasize the efficient implementation of these methods on both homogeneous and heterogeneous high-performance computing facilities.
More than a billion people worldwide may be selenium (Se) deficient, and supplementation with Se-rich Brazil nuts may be a good strategy to prevent deficiency. Since different forms of Se have different nutritional value, and Se is toxic at elevated levels, careful seed characterization is important. Variation in Se concentration and correlations of this element with other nutrients were found in two batches of commercially available nuts. Selenium tissue localization and specification were further determined. Mean Se levels were between 28 and 49 mg kg-1, with up to 8-fold seed-to-seed variation (n = 13) within batches. Brazil nut Se was mainly in organic form. While present throughout the seed, Se was most concentrated in a ring 1 to 2 mm below the surface. While healthy, Brazil nuts should be consumed in moderation. Consumption of one seed (5 g) from a high-Se area meets its recommended daily allowance; the recommended serving size of 30 g may exceed the allowable daily intake (400 μg) or even its toxicity threshold (1200 μg). Based on these findings, the recommended serving size may be re-evaluated, consumers should be warned not to exceed the serving size and the seed may be sold as part of mixed nuts, to avoid excess Se intake.
Mutations in the human BEST1 gene lead to retinal degenerative diseases displaying progressive vision loss and even blindness. BESTROPHIN1, encoded by BEST1, is predominantly expressed in retinal pigment epithelium (RPE), but its physiological role has been a mystery for the last two decades. Using a patient-specific iPSC-based disease model and interdisciplinary approaches, we comprehensively analyzed two distinct BEST1 patient mutations, and discovered mechanistic correlations between patient clinical phenotypes, electrophysiology in their RPEs, and the structure and function of BESTROPHIN1 mutant channels. Our results revealed that the disease-causing mechanism of BEST1 mutations is centered on the indispensable role of BESTROPHIN1 in mediating the long speculated Ca2+-dependent Cl- current in RPE, and demonstrate that the pathological potential of BEST1 mutations can be evaluated and predicted with our iPSC-based ‘disease-in-a-dish’ approach. Moreover, we demonstrated that patient RPE is rescuable with viral gene supplementation, providing a proof-of-concept for curing BEST1-associated diseases.
Cytokines elicit pleiotropic and non-redundant activities despite strong overlap in their usage of receptors, JAKs and STATs molecules. We use IL-6 and IL-27 to ask how two cytokines activating the same signaling pathway have different biological roles. We found that IL-27 induces more sustained STAT1 phosphorylation than IL-6, with the two cytokines inducing comparable levels of STAT3 phosphorylation. Mathematical and statistical modeling of IL-6 and IL-27 signaling identified STAT3 binding to GP130, and STAT1 binding to IL-27Rα, as the main dynamical processes contributing to sustained pSTAT1 levels by IL-27. Mutation of Tyr613 on IL-27Rα decreased IL-27-induced STAT1 phosphorylation by 80% but had limited effect on STAT3 phosphorgylation. Strong receptor/STAT coupling by IL-27 initiated a unique gene expression program, which required sustained STAT1 phosphorylation and IRF1 expression and was enriched in classical Interferon Stimulated Genes. Interestingly, the STAT/receptor coupling exhibited by IL-6/IL-27 was altered in patients with systemic lupus erythematosus (SLE). IL-6/IL-27 induced a more potent STAT1 activation in SLE patients than in healthy controls, which correlated with higher STAT1 expression in these patients. Partial inhibition of JAK activation by sub-saturating doses of Tofacitinib specifically lowered the levels of STAT1 activation by IL-6. Our data show that receptor and STATs concentrations critically contribute to shape cytokine responses and generate functional pleiotropy in health and disease.
An analytical study of stresses in the blades recorded during the tests of the DH 2011 jet flap rotor was performed. The main objective of the study was to compare the experimental results with analyticaly determined stresses. The comparison extended over 15 specific flight cases has been only partially successful. In fact computed 3P and 4P stress components showed only a poor correlation with the test data obtained. It is believed that the simplified model of aeroelastic effects used is mainly responsible for this lack of agreement with test results.
The author has identified the following significant results. Data for Assateague Island and Ocracoke Island show significantly high correlations between coastal orientation and erosion. However, South Hatteras has low correlations, and North Hatteras has low negative correlations. Because all four areas are essentially in the same mid-Atlantic climatic regime, process/response relationships should be similar. Correlations for the four areas may differ considerably because of inadequate assumptions; the key is the mean orientation of the specific coastline being studied. There appears to be an orientation window surrounding due northeast (135 deg north of south) in which this hypothesis holds.
A design package is presented for the specification of acoustic liners for turbofans. An estimate of the noise generation was made based on modifications of existing noise correlations, for which the inputs are basic fan aerodynamic design variables. The method does not predict multiple pure tones. A target attenuation spectrum was calculated which was the difference between the estimated generation spectrum and a flat annoyance-weighted goal attenuated spectrum. The target spectrum was combined with a knowledge of acoustic liner performance as a function of the liner design variables to specify the acoustic design. The liner design method at present is limited to annular duct configurations. The detailed structure of the liner was specified by combining the required impedance (which is a result of the previous step) with a mathematical model relating impedance to the detailed structure. The design procedure was developed for a liner constructed of perforated sheet placed over honeycomb backing cavities. A sample calculation was carried through in order to demonstrate the design procedure, and experimental results presented show good agreement with the calculated results of the method.
A literature review was conducted to assess the basis of and techniques for physiological assessment of mental workload. The study findings reviewed had shortcomings involving one or more of the following basic problems: (1) physiologic arousal can be easily driven by nonworkload factors, confounding any proposed metric; (2) the profound absence of underlying physiologic models has promulgated a multiplicity of seemingly arbitrary signal processing techniques; (3) the unspecified multidimensional nature of physiological "state" has given rise to a broad spectrum of competing noncommensurate metrics; and (4) the lack of an adequate definition of workload compels physiologic correlations to suffer either from the vagueness of implicit workload measures or from the variance of explicit subjective assessments. Using specific studies as examples, two basic signal processing/data reduction techniques in current use, time and ensemble averaging are discussed.
Ultrasonic polar backscatter, a quantitative NDE technique introduced by O'Donnell and Miller (1981), was used to characterize impact damages in a 16-ply graphite-epoxy laminate. The results were correlated with the results obtained by the destructive deply technique of Freeman (1984). The size, shape, and orientation of damage correlated well between the polar backscatter technique and the deply technique. There was good quantitative correlation between the areas of damage indicated by the two techniques, suggesting that the polar backscatter technique is sensitive to specific orientations of damage. The polar backscatter technique provides a good qualitative image of the size and shape of the largest zone of damage in each of the principal orientations; a quantitative estimate of the extent of these largest damage zones can then be obtained.
A study of the primary scintillation light in liquid xenon excited by 241 Am alpha particles and 207 Bi internal conversion electrons are discussed. The time dependence and the intensity of the light at different field strengths have been measured with a specifically designed chamber, equipped with a CaF sub 2 light transmitting window coupled to a UV sensitive PMT. The time correlation between the fast light signal and the charge signal shows that the scintillation signals produced in liquid xenon by ionizing particles provides an ideal trigger in a Time Projection type LXe detector aiming at full imaging of complex gamma-ray events. Researchers also started Monte Carlo calculations to establish the performance of a LXe imaging telescope for high energy gamma-rays.
A study of the primary scintillation light in liquid xenon excited by 241 Am alpha particles and 207 Bi internal conversion electrons are discussed. The time dependence and the intensity of the light at different field strengths have been measured with a specifically designed chamber, equipped with a CaF sub 2 light transmitting window coupled to a UV sensitive PMT. The time correlation between the fast light signal and the charge signal shows that the scintillation signals produced in liquid xenon by ionizing particles provides an ideal trigger in a Time Projection type LXe detector aiming at full imaging of complex gamma-ray events. Researchers also started Monte Carlo calculations to establish the performance of a LXe imaging telescope for high energy gamma-rays.
The Jet Propulsion Laboratory (JPL) has undertaken a task to relate long duration, space flight hardware performance to specific product assurance requirements established during the hardware development process. This paper describes the approach that JPL is using to correlate in-flight and ground test hardware failures to critical product assurance practices implemented on a given flight project. The first step in this effort has been to collect, and convert into a convenient format, in-flight problem, failure, and anomaly data. A characterization of a subset of this anomaly data base, focusing on anomaly causes, time dependence, and subsystem affected is presented.
This paper is written for the purpose of providing an introduction and set of guidelines for the use of a methodology for NASTRAN eigenvalue modeling of thin film inflatable structures. It is hoped that this paper will spare the reader from the problems and headaches the authors were confronted with during their investigation by presenting here not only an introduction and verification of the methodology, but also a discussion of the problems that this methodology can ensue. Our goal in this investigation was to verify the basic methodology through the creation and correlation of a simple model. An overview of thin film structures, their history, and their applications is given. Previous modeling work is then briefly discussed. An introduction is then given for the method of modeling. The specific mechanics of the method are then discussed in parallel with a basic discussion of NASTRAN s implementation of these mechanics. The problems encountered with the method are then given along with suggestions for their work-a-rounds. The methodology is verified through the correlation between an analytical model and modal test results of a thin film strut. Recommendations are given for the needed advancement of our understanding of this method and ability to accurately model thin film structures. Finally, conclusions are drawn regarding the usefulness of the methodology.
During the time period covered by this report, SAIC: 1) Continued to develop and assess processes and approaches that can be applied to Second Generation Reuseable Launch Vehicles (RLV) technologies prioritization. An approach based on the use of analytic Saaty scale functions has been defined and is being investigated. 2) Planned and facilitated technologies prioritization workshops, supported development of systems program algorithms based on the concept of influence diagramming, and assessment of analogies between aircraft and space systems developments. 3) Video interviews held with X-37 personnel at Dryden. The CD-ROM is being concluded and a near-final review disc is expected soon. 4) CD-ROMS were produced by Engineered Multimedia, Inc. (EMI) under subcontract to SAIC. These two CD-ROM products, "Microgravity" and "New Horizons", were delivered to MSFS technical representatives at a briefing at SAID on January 18, 2001. 5) Presentation brochure, "Aviation/Space Analog Team Interim Report" was provided to Space Propulsion Synergy Team (SPST) members and to NASA personnel. Wrap-up of effort will be a mid-April briefing to both MSFC and the full SPST membership. Support to Phase 2 of CCPD ends 3/31/01. Phase 3 continuation effort planned with emphasis on technical info content. 6) Recommended that eliminating the B-52 flights of the X-37 in favor of alternate approached be evaluated. Discovered that a required change to the thruster valves of the X-37 had not been made. Repair work continues on the lower fuselage section of the X-37, in the areas that experienced core collapse. The currently catalogued potential weight increases and decreases are about equal, but this does not include the impact of the cable weight underestimate reported earlier. The CFD for RCS testing correlates well with the wind tunnel data. 7) The Level IV CCB approved SCN-4 to the MC-1 Engine SPecification. Approved changes have been incorporated into the specification, and Revision D has been released to the Repository. Billy Gonterman, SAIC, traveled to SSC to perform a five-day readiness assessment on the progress of facilities activation. Implemented modifications to the MC-1 Engine TPA Component Database. 8) Replaced 25 Combined-Cycle Propulsion Database (CCPD) documents; modified the low-on screen to include a direct email link for user contact; prepared a summary presentation of current and proposed tasks.