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At least 1,405 records · Page 78

Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6

Significance The loading of the core Mcm2-7 helicase onto origin DNA is essential for the formation of replication forks and genomic stability. Here, we report two cryo-electron microscopy (cryo-EM) structures that capture helicase loader–helicase complexes just prior to DNA insertion. These pre-loading structures, combined with a computational simulation of the dynamic transition from the pre-loading state to the loaded state, provide crucial insights into the mechanism required for topologically linking the helicase to DNA. The helicase loading system is highly conserved from yeast to human, which means that the molecular principles described here for the yeast system are likely applicable to the human system.

59 BASIC BIOLOGICAL SCIENCES↗

Results from tests of three prototype general aviation seats

Three types of energy absorbing general aviation seats were dynamically tested and evaluated for crash load attenuation. On the basis of the static and dynamic test results, it was recommended that the tubular frame seats be redesigned to initiate stroking at approximately 12 G's rather than the 20 to 25 G range. Lower density foam was recommended for the foam wedge passenger seat.

Williams, M. S.↗

Evaluation of Load Analysis Methods for NASAs GIII Adaptive Compliant Trailing Edge Project

The Air Force Research Laboratory (AFRL), NASA Armstrong Flight Research Center (AFRC), and FlexSys Inc. (Ann Arbor, Michigan) have collaborated to flight test the Adaptive Compliant Trailing Edge (ACTE) flaps. These flaps were installed on a Gulfstream Aerospace Corporation (GAC) GIII aircraft and tested at AFRC at various deflection angles over a range of flight conditions. External aerodynamic and inertial load analyses were conducted with the intention to ensure that the change in wing loads due to the deployed ACTE flap did not overload the existing baseline GIII wing box structure. The objective of this paper was to substantiate the analysis tools used for predicting wing loads at AFRC. Computational fluid dynamics (CFD) models and distributed mass inertial models were developed for predicting the loads on the wing. The analysis tools included TRANAIR (full potential) and CMARC (panel) models. Aerodynamic pressure data from the analysis codes were validated against static pressure port data collected in-flight. Combined results from the CFD predictions and the inertial load analysis were used to predict the normal force, bending moment, and torque loads on the wing. Wing loads obtained from calibrated strain gages installed on the wing were used for substantiation of the load prediction tools. The load predictions exhibited good agreement compared to the flight load results obtained from calibrated strain gage measurements.

Aerodynamic Loads↗

Quantification of energy and cost reduction from decreasing dissolved oxygen levels in full‐scale water resource recovery facilities

Abstract Aeration systems often lack the efficiency to maintain a desired residual dissolved oxygen (DO) concentration in the tank in part because little consideration is given to the dynamic daily and seasonal loading conditions. Although advanced aeration controllers exist, the majority of plants have DO set points typically based on common practice and literature values rather than site‐specific conditions, which can result in DO set points higher than those necessary to meet treatment objectives. DO set point reduction strategies have primarily been proposed through either static or dynamic simulations. In this study, the substantial improvements associated with DO set point reduction are demonstrated at full scale. A yearlong characterization of full‐scale aeration dynamics captured the effect of diurnal and seasonal fluctuations on oxygen transfer and energy demand and so facilitated the estimation of the potential savings of DO reduction strategies. Full‐scale validation provided direct evidence of DO reduction strategies inducing an overall enhancement of oxygen transfer efficiency along the different bioreactors, while confirming that energy savings as high as 20% were feasible. This study quantifies the influence of oxygen transfer efficiency on operating choices and site‐specific conditions (control strategy, loading conditions, and influent flow variability). Practitioner points We quantified the energy reduction and cost savings associated with a DO reduction in an aeration tank. For each 0.2 mg/L of DO decreased, the average power demand reduction per unit water treated exceeded 17%. Field measurements of dynamic alpha values eliminate the uncertainty in estimating aeration energy and cost savings from DO variations.

Pasini, Federico↗

Accelerated testing of composites

It is shown that the Zhurkov method for testing the strength of solids can be applied to dynamic tension and to cyclic loading and provides a viable approach to accelerated testing of composites. Data from the literature are used to demonstrate a straightforward application of the method to dynamic tension of glass fiber and cyclic loading for glass/polymer, metal matrix, and graphite/epoxy composites. Zhurkov's equation can be used at relatively high loads to obtain failure times at any temperature of interest. By taking a few data points at one or two other temperatures the spectrum of failure times can be expanded to temperatures not easily accessible.

Papazian, H. A.↗

Dynamic analysis of straight and involute tooth forms

The effect of load speed on straight and involute tooth forms is studied using several finite-element models. It is found that for rapidly rotating gears and sprockets, the load speed along the tooth surface can significantly affect the tooth vibration. Indeed, it is found that for sufficiently high load speeds and for sufficiently slender tooth forms, the tooth deflection can, at times, be directed opposite to the load direction. Comparisons are made of various dynamic models of gear and sprocket teeth. It is shown that for stubby tooth forms there is considerable difference between results obtained with finite element models and results obtained with Timoshenko beam models. Finally, it is shown that gear or sprocket vibrations can be induced by the shape of the tooth form itself. This effect becomes increasingly significant at higher speeds.

Lin, H. H.↗

Adaptive control of dual-arm robots

Three strategies for adaptive control of cooperative dual-arm robots are described. In the position-position control strategy, the adaptive controllers ensure that the end-effector positions of both arms track desired trajectories in Cartesian space despite unknown time-varying interaction forces exerted through the load. In the position-hybrid control strategy, the adaptive controller of one arm controls end-effector motions in the free directions and applied forces in the constraint directions, while the adaptive controller of the other arm ensures that the end-effector tracks desired position trajectories. In the hybrid-hybrid control strategy, the adaptive controllers ensure that both end-effectors track reference position trajectories while simultaneously applying desired forces on the load. In all three control strategies, the cross-coupling effects between the arms are treated as disturbances which are rejected by the adaptive controllers while following desired commands in a common frame of reference. The adaptive controllers do not require the complex mathematical model of the arm dynamics or any knowledge of the arm dynamic parameters or the load parameters such as mass and stiffness. The controllers have simple structures and are computationally fast for on-line implementation with high sampling rates.

Seraji, H.↗

Dual-arm manipulators with adaptive control

The described and improved multi-arm invention of this application presents three strategies for adaptive control of cooperative multi-arm robots which coordinate control over a common load. In the position-position control strategy, the adaptive controllers ensure that the end-effector positions of both arms track desired trajectories in Cartesian space despite unknown time-varying interaction forces exerted through a load. In the position-hybrid control strategy, the adaptive controller of one arm controls end-effector motions in the free directions and applied forces in the constraint directions; while the adaptive controller of the other arm ensures that the end-effector tracks desired position trajectories. In the hybrid-hybrid control strategy, the adaptive controllers ensure that both end-effectors track reference position trajectories while simultaneously applying desired forces on the load. In all three control strategies, the cross-coupling effects between the arms are treated as disturbances which are compensated for by the adaptive controllers while following desired commands in a common frame of reference. The adaptive controllers do not require the complex mathematical model of the arm dynamics or any knowledge of the arm dynamic parameters or the load parameters such as mass and stiffness. Circuits in the adaptive feedback and feedforward controllers are varied by novel adaptation laws.

Seraji, Homayoun↗

Method and apparatus for adaptive force and position control of manipulators

The described and improved multi-arm invention of this application presents three strategies for adaptive control of cooperative multi-arm robots which coordinate control over a common load. In the position-position control strategy, the adaptive controllers ensure that the end-effector positions of both arms track desired trajectories in Cartesian space despite unknown time-varying interaction forces exerted through a load. In the position-hybrid control strategy, the adaptive controller of one arm controls end-effector motions in the free directions and applied forces in the constraint directions; while the adaptive controller of the other arm ensures that the end-effector tracks desired position trajectories. In the hybrid-hybrid control strategy, the adaptive controllers ensure that both end-effectors track reference position trajectories while simultaneously applying desired forces on the load. In all three control strategies, the cross-coupling effects between the arms are treated as disturbances which are compensated for by the adaptive controllers while following desired commands in a common frame of reference. The adaptive controllers do not require the complex mathematical model of the arm dynamics or any knowledge of the arm dynamic parameters or the load parameters such as mass and stiffness. Circuits in the adaptive feedback and feedforward controllers are varied by novel adaptation laws.

Seraji, Homayoun↗

Robust Real-Time Modeling of Distribution Systems with Data-Driven Grid-Wise Observability (Final Technical Report)

The overall objective of this project is to leverage existing and emerging sensor measurements to develop data-driven observability enhancement algorithms as well as robust state estimation and parameter identification techniques to enable real-time grid-wise monitoring and modeling of loads and distributed energy resources (DERs). The project resulted in a holistic framework with the following key components: 1) data-driven and machine learning-based grid-edge visibility enhancement, 2) robust branch-current-based state estimation (BCSE), and 3) robust real-time steady-state and dynamic-state modeling of loads and DERs. The research outcomes have provided utility companies better network visibility, higher-fidelity load/DER models, and more accurate assessment of DERs’ impacts, thus, facilitating the integration of renewable energy sources. The synergistic collaborative project among Iowa State University (ISU), Argonne National Laboratory (ANL), Electric Power Research Center (EPRC), SIEMENS Industry, Alliant Energy, Cedar Falls Utilities (CFU), and Maquoketa Valley Electric Cooperative (MVEC) to leverage the team’s extensive expertise and experience in power distribution systems, state estimation, online modeling and identification, and DER integrations. The proposed frameworks have been verified with industry adopted software and attempted integrated with existing tool wherever possible

24 POWER TRANSMISSION AND DISTRIBUTION↗

Investigation of prediction methods for the loads and stresses of Apollo type spacecraft parachutes. Volume 1: Loads

An analysis was conducted with the objective of upgrading and improving the loads, stress, and performance prediction methods for Apollo spacecraft parachutes. The subjects considered were: (1) methods for a new theoretical approach to the parachute opening process, (2) new experimental-analytical techniques to improve the measurement of pressures, stresses, and strains in inflight parachutes, and (3) a numerical method for analyzing the dynamical behavior of rapidly loaded pilot chute risers.

Mickey, F. E.↗

Space Station flexible dynamics under plume impingement

Assembly of the Space Station requires numerous construction flights by the Space Shuttle. A particularly challenging problem is that of control of each intermediate station configuration when the shuttle orbiter is approaching it to deliver the next component. The necessary braking maneuvers cause orbiter thruster plumes to impinge on the station, especially its solar arrays. This in turn causes both overall attitude errors and excitation of flexible-body vibration modes. These plume loads are predicted to lead to CMG saturation during the approach of the orbiter to the SC-5 station configuration, necessitating the use of the station RCS jets for desaturation. They are also expected to lead to significant excitation of solar array vibrations. It is therefore of great practical importance to investigate the effects of plume loads on the flexible dynamics of station configuration SC-5 as accurately as possible. However, this system possesses a great many flexible modes (89 below 5 rad/s), making analysis time-consuming and complicated. Model reduction techniques can be used to overcome this problem, reducing the system model to one which retains only the significant dynamics, i.e. those which are strongly excited by the control inputs or plume disturbance forces and which strongly couple with the measured outputs. The particular technique to be used in this study is the subsystem balancing approach which was previously developed by the present investigator. This method is very efficient computationally. Furthermore, it gives accurate results even for the difficult case where the structure has many closed-spaced natural frequencies, when standard modal truncation can give misleading results. Station configuration SC-5 is a good example of such a structure.

Williams, Trevor↗

Dynamic testing of docking system hardware

Extensive dynamic testing was conducted to verify the flight readiness of the Apollo docking hardware. Testing was performed on a unique six degree-of-freedom motion simulator controlled by a computer that calculated the associated spacecraft motions. The test system and the results obtained by subjecting flight-type docking hardware to actual impact loads and resultant spacecraft dynamics are described.

Dorland, W. D.↗

Reducing heat load density with asymmetric and inclined double-crystal monochromators: principles and requirements revisited

Asymmetric double-crystal monochromators (aDCMs) and inclined DCMs (iDCMs) can significantly expand the X-ray beam footprint and consequently reduce the heat load density and gradient. Based on rigorous dynamical theory calculations, the major principles and properties of aDCMs and iDCMs are presented to guide their design and development, particularly for fourth-generation synchrotrons. In addition to the large beam footprint, aDCMs have very large bandwidths (up to ∼10 eV) and angular acceptance, but the narrow angular acceptance of the second crystal requires precise control of the relative orientations and strains. Based on Fourier coupled-wave diffraction theory calculations, it is rigorously proved that the iDCM has almost the same properties as the conventional symmetric DCM, including the efficiency, angular acceptance, bandwidth, tuning energy range and sensitivity to misalignment. The exception is that, for the extremely inclined geometry that can achieve very large footprint expansion, the iDCM has (beneficially) a larger bandwidth and wider angular acceptance. Inclined diffraction has the `rho-kick effect' that can be cancelled by the second reflection of the iDCM (even with misalignment), except that inhomogeneous strains may cause non-uniform rho-kick angles. At present, fabrication/mounting-induced strains pose low risk since they can be controlled to <0.5 µrad over large areas. The only uncertain challenge is the thermally induced strains, yet it is estimated that these strains are naturally lowered by the large footprint and may be further mitigated by optimized cryogenic cooling to the 1–2 µrad level. Overall, aDCMs and iDCMs have more stringent requirements than normal DCMs, but they are feasible schemes in practice.

asymmetric monochromator↗

Ares I-X Flight Test Vehicle Modal Test

The first test flight of NASA's Ares I crew launch vehicle, called Ares I-X, was launched on October 28, 2009. Ares I-X used a 4-segment reusable solid rocket booster from the Space Shuttle heritage with mass simulators for the 5th segment, upper stage, crew module and launch abort system. Flight test data will provide important information on ascent loads, vehicle control, separation, and first stage reentry dynamics. As part of hardware verification, a series of modal tests were designed to verify the dynamic finite element model (FEM) used in loads assessments and flight control evaluations. Based on flight control system studies, the critical modes were the first three free-free bending mode pairs. Since a test of the free-free vehicle was not practical within project constraints, modal tests for several configurations during vehicle stacking were defined to calibrate the FEM. Test configurations included two partial stacks and the full Ares I-X flight test vehicle on the Mobile Launcher Platform. This report describes the test requirements, constraints, pre-test analysis, test execution and results for the Ares I-X flight test vehicle modal test on the Mobile Launcher Platform. Initial comparisons between pre-test predictions and test data are also presented.

Buehrle, Ralph D.↗