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At least 1,405 records · Page 78

KBase Narrative - Atacama Trench & Sn Clemente 2018 cruise Metagenomes -Decontamination through Metagenomic Fractionation and analysis of Metabolic Potential-

Deep-sea microbiomes sampled with traditional or pressure-retaining instrumentation A fundamental property of life in the sea is that with increasing depth must come increased adaptation to elevated hydrostatic pressures. This project aims to assess technology for the accurate characterization of microbial biodiversity and biochemistry at greater depths, and validate its use in some of the deepest and least-explored waters in the Ocean: Hadal Trench. Compared to the 0.1 megapascal (MPa) of surface waters, organisms inhabiting at the average depth of the Ocean (3800 m) have to deal with pressures of 38 MPa (5,500 psi). The effects of these high pressure have been most thoroughly investigated in microorganisms, where it has been found to affect membrane integrity and transport, cytoskeletal assembly and both DNA and protein synthesis. We will explore the diversity and activity of microorganisms from 2000 m to >8,000 m depths within the highly productive water column of hadal trenches, comparing seawater samples obtained with and without substantial pressure changes during recovery. A pressure retaining device was used to sample the microbial communities of the watercolumn with reduced decompression during retreival (Peoples et. al., 2019). References: Peoples LM, Norenberg M, Price D, McGoldrick M, Novotny M, Bochdansky A, Bartlett DH. A full-ocean-depth rated modular lander and pressure-retaining sampler capable of collecting hadal-endemic microbes under in situ conditions. Deep Sea Research Part I: Oceanographic Research Papers. 2019 Jan 1;143:50-7.

Plominsky, Alvaro M.↗

KBase Narrative - Atacama Trench & Sn Clemente 2018 cruise Metagenomes -Sequence quality filtration & Assembly-

Deep-sea microbiomes sampled with traditional or pressure-retaining instrumentation A fundamental property of life in the sea is that with increasing depth must come increased adaptation to elevated hydrostatic pressures. This project aims to assess technology for the accurate characterization of microbial biodiversity and biochemistry at greater depths, and validate its use in some of the deepest and least-explored waters in the Ocean: Hadal Trench. Compared to the 0.1 megapascal (MPa) of surface waters, organisms inhabiting at the average depth of the Ocean (3800 m) have to deal with pressures of 38 MPa (5,500 psi). The effects of these high pressure have been most thoroughly investigated in microorganisms, where it has been found to affect membrane integrity and transport, cytoskeletal assembly and both DNA and protein synthesis. We will explore the diversity and activity of microorganisms from 2000 m to >8,000 m depths within the highly productive water column of hadal trenches, comparing seawater samples obtained with and without substantial pressure changes during recovery. A pressure retaining device was used to sample the microbial communities of the watercolumn with reduced decompression during retreival (Peoples et. al., 2019). References: Peoples LM, Norenberg M, Price D, McGoldrick M, Novotny M, Bochdansky A, Bartlett DH. A full-ocean-depth rated modular lander and pressure-retaining sampler capable of collecting hadal-endemic microbes under in situ conditions. Deep Sea Research Part I: Oceanographic Research Papers. 2019 Jan 1;143:50-7.

Plominsky, Alvaro M.↗

Cosmogenic radionuclides in the Allende and Murchison carbonaceous chondrites.

Measurements were made of Na-22, Al-26, Mn-54, and Co-60 produced by cosmic rays in five samples of the Allende C3 meteorite and in one specimen of Murchison (C2); Sc-46, V-48, Cr-51, and Co-57 were also measured in several of these samples. Comparison of observed Allende Co-60 activities with calculated neutron-capture production rates yields a depth scale for Allende that appears unambiguous to about 30-cm depth. Using this scale, the production of Al-26 is constant to a depth in excess of 30 cm, Na-22 activity increases 30% from near the surface of Allende to 20- to 30-cm depth, and Mn-54 production increases 50% over the same depth range.

Cressy, P. J., Jr.↗

Ar-40/Ar-36 variations in Apollo 15 and 16 regolith

Values of Ar-40/Ar-36 have been determined for a large number of Apollo 15 and Apollo 16 fines, including samples from a range of depths in the Apollo 15 and 16 deep drill cores. Most of the Ar-40 in these samples is 'excess Ar-40' in that it has not been produced by in situ decay of K. Values of Ar-40/Ar-36 range from 0.74 to 4.35 and demonstrate that the regolith is not well mixed over depths of several cm or over surface distances greater than about 100 m. However, fines collected over surface distances of less than a few tens of meters tend to possess similar ratios. Values of Ar-40/Ar-36 in the Apollo 15 drill core increase with depth by nearly a factor of four (0.74 to 2.86) in the 0 to 80 cm depth range, then decrease for greater depths. Values of Ar-40/Ar-36 in the Apollo 16 drill core are nearly constant at about 1.3, except for the deepest sample analyzed (about 2.7). Concentrations of glass-bonded agglutinate particles in the fines tend to correlate with lower Ar-40/Ar-36, and may indicate that dynamic regolith processes also affect the trapped Ar-40/Ar-36.

Bogard, D. D.↗

In-situ measurement of the rate of U-235 fission induced by lunar neutrons

The depth profile of the neutron-induced fission rate of U-235 was directly measured to a depth of 350 g/sq cm by the Apollo 17 Lunar Neutron Probe Experiment. The fission rate rises sharply from the surface to a broad maximum from 110 to 160 g/sq cm and drops off at greater depths. The shape of the theoretical depth profile of Lingenfelter et al (1972) fits the measured capture rates well at all depths. The absolute magnitude of the experimental fission rates are (11 plus or minus 17)% lower than those calculated theoretically. The excellent agreement between theory and experiment implies that conclusions drawn previously by interpreting lunar sample data with the theoretical capture rates will not require revision. In particular, lunar surface processes, rather than uncertainties in the capture rates, are required to explain the relatively low neutron fluences observed for surface soil samples compared to the fluences expected for a uniformly mixed regolith.

Woolum, D. S.↗

Development of an airborne laser bathymeter

An airborne laser depth sounding system was built and taken through a complete series of field tests. Two green laser sources were tried: a pulsed neon laser at 540 nm and a frequency-doubled Nd:YAG transmitter at 532 nm. To obtain a depth resolution of better than 20 cm, the pulses had a duration of 5 to 7 nanoseconds and could be fired up to at rates of 50 pulses per second. In the receiver, the signal was detected by a photomultiplier tube connected to a 28 cm diameter Cassegrainian telescope that was aimed vertically downward. Oscilloscopic traces of the signal reflected from the sea surface and the ocean floor could either be recorded by a movie camera on 35 mm film or digitized into 500 discrete channels of information and stored on magnetic tape, from which depth information could be extracted. An aerial color movie camera recorded the geographic footprint while a boat crew of oceanographers measured depth and other relevant water parameters. About two hundred hours of flight time on the NASA C-54 airplane in the area of Chincoteague, Virginia, the Chesapeake Bay, and in Key West, Florida, have yielded information on the actual operating conditions of such a system and helped to optimize the design. One can predict the maximum depth attainable in a mission by measuring the effective attenuation coefficient in flight. This quantity is four times smaller than the usual narrow beam attenuation coefficient. Several square miles of a varied underwater landscape were also mapped.

Kim, H., H.↗

Nearshore coastal mapping

Two test sites of different water quality and bottom topography were used to test for maximum water depth penetration using the Skylab S-192 MSS for measurement of nearshore coastal bathymetry. Sites under investigation lie along the Lake Michigan coastline where littoral transport acts to erode sand bluffs and endangers developments along 1,200 miles of shore, and on the west coast of Puerto Rico where unreliable shoal location and depth information constitutes a safety hazard to navigation. The S-192 and S-190A and B provide data on underwater features because of water transparency in the blue/green portion of the spectrum. Depth of 20 meters were measured with the S-192 in the Puerto Rico test site. The S-190B photography with its improved spatial resolution clearly delineates the triple sand bar topography in the Lake Michigan test site. Several processing techniques were employed to test for maximum depth measurement with least error. The results are useful for helping to determine an optimum spectral bandwidth for future space sensors that will increase depth measurements for different water attenuation conditions where a bottom reflection is detectable.

Polcyn, F. C.↗

Hydrographic charting from LANDSAT Satellite: A comparison with aircraft imagery

The relative capabilities of two remote-sensing systems in measuring depth and, consequently, bottom contours in sandy-bottomed and sediment-laden coastal waters were determined quantitatively. The multispectral scanner (MSS), orbited on the LANDSAT-2 Satellite, and the ocean color scanner (OCS), flown on U-2 aircraft, were used. Analysis of imagery taken simultaneously indicates a potential for hydrographic charting of marine coastal and shallow shelf areas, even when water turbidity is a factor. Several of the eight optical channels examined on the OCS were found to be sensitive to depth or depth-related information. The greatest sensitivity was in OCS-4(0.544 + or - 0.012 microns) from which contours corresponding to depths up to 12m were determined. The sharpness of these contours and their spatial stability through time suggests that upwelling radiance is a measure of bottom reflectance and not of water turbidity. The two visible channels on LANDSAT's MSS were less sensitive in the discrimination of contours, with depths up to 8m in the high-gain mode (3x) determined in MSS-4(0.5 to 0.6 microns).

Middleton, E. M.↗

Lunar temperature and global heat flux from laboratory electrical conductivity and lunar magnetometer data

Three-layer monotonic electrical conductivity models for the lunar interior to a depth of 600 km are used in conjunction with laboratory measurements of the electrical conductivity of olivine and pyroxene to estimate a temperature-depth profile. The temperatures calculated for depths of 400-600 km are consistent with attenuation of the seismic shear wave. The temperature calculated at a depth of 100-250 km yields a heat flow that is in good agreement with the directly measured lunar heat flow. The temperature, however, is sufficiently close to melting that mascon anisostasy would not be maintained. Thus a better conductor is required at this depth.

Sonett, C. P.↗

Hydrographic charting from Landsat satellite - A comparison with aircraft imagery

The relative capabilities of two remote-sensing systems in measuring depth and, consequently, bottom contours in sandy-bottomed and sediment-laden coastal waters were determined quantitatively. The Multispectral Scanner (MSS), orbited on the Landsat-2 satellite, and the Ocean Color Scanner (OCS), flown on U-2 aircraft, were used for this evaluation. Analysis of imagery taken simultaneously indicates a potential for hydrographic charting of marine coastal and shallow shelf areas, even when water turbidity is a factor. Several of the eight optical channels examined on the OCS were found to be sensitive to depth or depth-related information. The greatest sensitivity was in OCS-4 (0.544 plus or minus 0.012 micron) from which contours corresponding to depths up to 12 m were determined. The sharpness of these contours and their spatial stability through time suggests that upwelling radiance is a measure of bottom reflectance and not of water turbidity. The two visible channels on Landsat's MSS were less sensitive in the discrimination of contours, with depths up to 8 m in the high-gain mode (3 X) determined in MSS-4 (0.5 to 0.6 micron).

Middleton, E. M.↗

Properties of the clouds of Venus, as inferred from airborne observations of its near-infrared reflectivity spectrum

The shape and absolute value of Venus' reflectivity spectrum is measured in the 1.2- to 4.0 micrometer spectral region with a circular variable filter wheel spectrometer having a spectral resolution of 1.5%. Comparing these spectra with synthetic spectra generated with a multiple-scattering computer code, a number of properties of the Venus clouds are inferred. Evidence is obtained indicating that the clouds are made of a water solution of sulfuric acid in their top unit optical depth, and that the clouds are made of this material down to an optical depth of at least 25. In addition, the acid concentration is 84 plus or minus 2% H2SO4 by weight in the top unit optical depth, the total optical depth of the clouds is 37.5 plus or minus 12.5, and the cross-sectional weighted mean particle radius lies between 0.5 and 1.4 micrometers in the top unit optical depth of the clouds. It is found that the average volume mixing ratio of H2SO4 and H2O contained in the cloud material both equal approximately 2 x 10 to the -6. Employing vapor pressure arguments, the acid concentration is shown to equal 84 plus or minus 6% at the cloud bottom and the water vapor mixing ratio beneath the clouds lies between 6 x 10 to the -4 and 10 to the -2.

Pollack, J. B.↗

Remote sensing of optical properties in continuously stratified waters

The radiative transfer equation is solved by Monte Carlo methods for natural waters in which the optical properties are distributed with depth. It is demonstrated that interpreting the reflectance of a continuously stratified ocean in terms of an equivalent homogeneous ocean yields the average of a particular combination of the water's optical properties over the dimensionless penetration depth. Although in general the dimensionless penetration depth cannot be remotely measured, a method is presented for estimating the actual penetration depth from the remote observations if the medium's absorption coefficient is known, independent of depth, and sufficiently large. The application of this to the remote measurement of the vertical distribution of suspended sediments is discussed in detail.

Gordon, H. R.↗

Natural lunar seismic events and the structure of the moon

The gross structure of the moon may be determined from natural seismic events, supplemented by artificial impacts at close ranges. The moon has a rigid mantle below a layered crust. At a depth of 500-850 km there is a decrease in S-velocity and an increase in attenuation; the preferred depth for this change is 600 km. The simultaneous occurrence of these phenomena indicates a small amount of melt below this depth. Deep focus moonquakes lie below the decrease in S-velocity at depths of about 650-950 km. Good estimates of the depth of shallow focus moonquakes are not available, but they may be releasing tectonic stress.

Dainty, A. M.↗

Fabrication of high efficiency and radiation resistant GaAs solar cells

Systematic improvements in fabrication yield were obtained by appropriate control of the liquid phase epitaxial growth process, contact fabrication and surface preparation. To improve radiation hardness, the junction depth was decreased while overcoming the penalty in decreased solar cell efficiency which tends to go hand-in-hand with the reduction of junction depth in (AlGa) As-GaAs solar cells. Cells were made with an AMO efficiency of 18% and a junction depth of 0.5 micrometers, as compared to junction depths on the order of 1.0 micrometers. With respect to the damage caused by proton irradiation, the nature of the observed damage was correlated to the energy and penetration depth of the damaging protons.

Kamath, S.↗

The Apollo 17 drill core - Petrologic systematics and the identification of a possible Tycho component

Modal data support a five-unit stratigraphy for the Apollo 17 drill core. The upper unit E (0-22 cm depth) is marked by high content of fused soil, brown glass, and mare basalt fragments. This unit corresponds with a portion of the core excavated and refilled within the last 2 m.y. The underlying unit D (22071 cm depth) has a low abundance of fused soil (i.e., low maturity) and is rich in coarse (less than 200 microns) mare fragments. A large section of the core, unit C (71-224 cm depth), is finer-grained, more mature (richer in agglutinates), more feldspathic and has more highland lithic, mineral and glass fragments than unit D. The next underlying unit, B (224-256 cm depth), has yellow/colorless KREEP glasses with a high Si, low-alkali composition unlike the common Apollo 15 or Apollo 17 KREEP series. The petrologic (fused soil) and Is/FeO maturity of this layer is also lower than the units above and below. The deepest unit, A (256-284 cm depth), is marked by its relatively higher maturity and lower yellow/colorless KREEP glass content. The most prominent petrographic/stratigraphic indicators are the pyroxene-rich immature mare unit D and the abundance of KREEP glass in unit B. This KREEP glass is distinctive petrographically and compositionally, and is probably exotic to the Apollo 17 site. It is suggested here that the KREEP glass in unit B is derived from Tycho, which implies widespread distribution of KREEP on the lunar nearside.

Vaniman, D. T.↗

Application of remote sensing techniques to hydrography with emphasis on bathymetry

Remote sensing techniques are utilized for the determination of hydrographic characteristics, with emphasis in bathymetry. Two sensor systems were utilized: the Metric Camera Wild RC-10 and the Multispectral Scanner of LANDSAT Satellite (MSS-LANDSAT). From photographs of the metric camera, data of photographic density of points with known depth are obtained. A correlation between the variables density x depth is calculated through a regression straight line. From this line, the depth of points with known photographic density is determined. The LANDSAT MSS images are interpreted automatically in the Iterative Multispectral Analysis System (I-100) with the obtention of point subareas with the same gray level. With some simplifications done, it is assumed that the depth of a point is directly related with its gray level. Subareas with points of the same depth are then determined and isobathymetric curves are drawn. The coast line is obtained through the sensor systems already mentioned. Advantages and limitations of the techniques and of the sensor systems utilized are discussed and the results are compared with ground truth.

Dejesusparada, N.↗

An analysis of haze effects on LANDSAT multispectral scanner data

Early season changes in optical depth change brightness, primarily along the soil line; and during crop development, changes in optical depth change both greenness and brightness. Thus, the existence of haze in the imagery could cause an unsuspecting analyst to interpret the spectral appearance as indicating an episodal event when, in fact, haze was present. The techniques for converting LANDSAT-3 data to simulate LANDSAT-2 data are in error. The yellowness and none such computations are affected primarily. Yellowness appears well correlated to optical depth. Experimental evidence with variable background and variable optical depth is needed, however. The variance of picture elements within a spring wheat field is related to its equivalent in optical depth changes caused by haze. This establishes the sensitivity of channel 1 (greenness) pixels to changes in haze levels. The between field picture element means and variances were determined for the spring wheat fields. This shows the variability of channel data on two specific dates, emphasizing that crop development can be influenced by many factors. The atmospheric correction program ATCOR reduces segment data from LANDSAT acquisitions to a common haze level and improves the results of analysis.

Johnson, W. R.↗

Solar activity during the past 10,000 years from radionuclides in lunar samples

The radionuclides Ar-37, Ar-39, H-3, and C-14 in lunar samples are related to solar activity during time periods corresponding to their mean lives. The Ar-37 activities at shallow depths vary with each Apollo mission according to the intensities of the large solar flares preceding the mission. At large depths, the Ar-37 activities are independent of solar flares and decrease with an attenuation length that is in accord with the energy spectrum of the galactic cosmic rays. The Ar-39 depth-profile differs somewhat from galactic cosmic-ray expectation; the difference may be caused by solar modulation during Maunder Minima periods. The H-3 and C-14 activities at shallow depths are considerably higher than expected from galactic cosmic rays. The high H-3 is explained by solar-flare interactions during the past 60 years. The high C-14 activities at shallow depths are caused in part by solar-flare interactions during the past 10,000 years and in part by solar-wind implantations. Solar wind-implanted C-14 is indicated by the grain-size and temperature-release dependence of C-14 in surface soil.

Fireman, E. L.↗