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Multispectral VNIR Observations by the Opportunity Rover Pancam of Multiple Episodes of Aqueous Alteration in Marathon Valley, Endeavour Crater, Mars

Since early 2015, the Mars Exploration Rover Opportunity has been exploring the break in the rim of Endeavour Crater dubbed Marathon Valley by the rover team. Marathon Valley was identified by orbital hyperspectral data from the MRO CRISM as having a relatively strong spectral feature in the 2.3 micrometer region indicative of an Mg or Fe-OH combination overtone absorption band indicative of smectite clay. Earlier in its mission, Opportunity examined the Matijevic Hill region on the more northerly Cape York crater rim segment and found evidence for smectite clays in a stratigraphically lower, pre-impact formed unit dubbed the Matijevic formation. However, the smectite exposures in Marathon Valley appear to be associated with the stratigraphically higher Shoemaker formation impact breccia. Evidence for alteration in this unit in Marathon Valley is provided by Pancam multispectral observations in the 430 to 1010 nm visible/near infrared (VNIR) spectral range. Sinuous troughs ("red zones") contain fragmented cobbles and pebbles displaying higher blue-to-red slopes, moderately higher 535 nm band depths, elevated 754 to 934 nm, and negative 934 to 1009 nm slopes. The lack of an absorption at 864 to 904 nm indicates the lack of crystalline red hematite in these red zones, but likely an enrichment in nanophase ferric oxides. The negative 934 to 1009 nm slope is potentially indicative of the presence of adsorbed or structurally bound water. A scuff in a red zone near the southern wall of Marathon Valley uncovered light-toned soils and a pebble with an 803 to 864 nm absorption resembling that of light-toned Fe-sulfate bearing soils uncovered by the Spirit rover in the Columbia Hills of Gusev crater. APXS chemical measurements indicated enrichments of Mg and S in the scuff soils and the pebble, Joseph Field, with the strongest 803 nm band- consistent with Mg and Fe sulfates. The presence of Fe and Mg sulfates can be interpreted as evidence of a potentially later episode of aqueous alteration with an earlier, neutral to alkaline pH episode forming the Fe/Mg smectites and a later acid pH episode forming the Fe and Mg sulfates.

Farrand, William H.↗

Transformational Challenge Reactor preconceptual core design studies

In the nuclear industry, a manufacturing-informed design approach has the potential to yield the most benefit from advanced manufacturing. By leveraging advanced materials, data science, and rapid testing and deployment, manufacturing-informed design can drive down costs and development times, ultimately improving future commercial viability. This approach is being demonstrated in the US Department of Energy Office of Nuclear Energy (DOE-NE) Transformational Challenge Reactor (TCR) program. Preconceptual design activities for TCR have been focused on analyzing and maturing four reactor core design concepts: two fast-spectrum and two thermal-spectrum systems. Furthermore, the designs were iteratively modified and analyzed, and subcomponents were manufactured in parallel over weeks instead of months or years. To meet key program initiatives (e.g., timeline and material use), several constraints—including fissile material availability, component availability, materials compatibility, and additive manufacturing capabilities—were factored into the design effort, yielding small cores less than one cubic meter in volume with near-term viability. Additionally, the TCR program has made significant progress on development of advanced moderator materials such as yttrium hydride, advancing the feasibility of gas-cooled thermal spectrum systems using less than 250 kg of high-assay low enriched uranium (HALEU) and occupying less than 1 m3. Each of the two resulting thermal designs uses a different fuel form: traditional UO2 ceramic fuel and tristructural isotropic (advanced TRISO) fuel particles embedded inside a SiC matrix. Core neutronics and thermal performance for these systems were assessed and summarized. Evaluation of the performance metrics for these two moderated designs has yielded the downselected TCR design: a TRISO-fueled and yttrium hydride moderated gas-cooled reactor.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

A multiparametric analysis of the Einstein sample of early-type galaxies. 2: Galaxy formation history and properties of the interstellar medium

We have conducted bivariate and multivariate statistical analysis of data measuring the integrated luminosity, shape, and potential depth of the Einstein sample of early-type galaxies (presented by Fabbiano et al. 1992). We find significant correlations between the X-ray properties and the axial ratios (a/b) of our sample, such that the roundest systems tend to have the highest L(sub x) and L(sub x)/L(sub B). The most radio-loud objects are also the roundest. We confirm the assertion of Bender et al. (1989) that galaxies with high L(sub x) are boxy (have negative a(sub 4)). Both a/b and a(sub 4) are correlated with L(sub B), but not with IRAS 12 um and 100 um luminosities. There are strong correlations between L(sub x), Mg(sub 2), and sigma(sub nu) in the sense that those systems with the deepest potential wells have the highest L(sub x) and Mg(sub 2). Thus the depth of the potential well appears to govern both the ability to reatin an ISM at the present epoch and to retain the enriched ejecta of early star formation bursts. Both L(sub x)/L(sub B) and L(sub 6) (the 6 cm radio luminosity) show threshold effects with sigma(sub nu) exhibiting sharp increases at log sigma(sub nu) approximately = 2.2. Finally, there is clearly an interrelationship between the various stellar and structural parameters: The scatter in the bivariate relationships between the shape parameters (a/b and a(sub 4)) and the depth parameter sigma(sub nu) is a function of abundance in the sense that, for a given a(sub 4) or a/b, the systems with the highest sigma(sub nu) also have the highest Mg(sub 2). Furthermore, for a constant sigma(sun nu), disky galaxies tend to have higher Mg(sub 2) than boxy ones. Alternatively, for a given abundance, boxy ellipticals tend to be more massive than disky ellipticals. One possibility is that early-type galaxies of a given mass, originating from mergers (boxy ellipticals), have lower abundances than 'primordial' (disky) early-type galaxies. Another is that disky inner isophotes are due not to primordial dissipation collapse, but to either the self-gravitating inner disks of captured spirals or the dissipational collapse of new disk structures from the premerger ISM. The high measured nuclear Mg(sub 2) values would thus be due to enrichment from secondary bursts of star formation triggered by the merging event.

Eskridge, Paul B.↗

Cortical and cap sedimentation in gravitropic Equisetum roots

Although the rootcap is required for gravitropic sensing, various classical and contemporary data raise the question of whether additional sensing occurs away from the cap in roots. Roots of Equisetum hyemale L. (horsetail) were examined by light and electron microscopy to determine which cell components were distributed with respect to gravity both in and away from the rootcap. Adventitious roots from stem cuttings were gravitropic in a vertical orientation or if reoriented to the horizontal. Obvious amyloplast sedimentation was found in vertical and in reoriented roots 1) in cells in the center of the rootcap and 2) in young, elongating cortical cells located in two to three layers outside the endodermis. These cortical amyloplasts were smaller than cap amyloplasts and, unlike central cap amyloplasts, were occasionally found in the top of the cell. The nucleus was also sedimented on top of the amyloplasts in both cell types, both in vertical and in reoriented roots. Sedimentation of both organelles ceased as cortical cells elongated further or as cap cells became peripheral in location. In both cell types with sedimentation, endoplasmic reticulum was located in the cell periphery, but showed no obvious enrichment near the lower part of the cell in vertical roots. This is the first modern report of sedimentation away from the cap in roots, and it provides structural evidence that gravitropic sensing may not be confined to the cap in all roots.

NASA Discipline Plant Biology↗

The Distribution of Methane in Titan's Stratosphere from Cassini/CIRS Observations

Cassini/CIRS spectra in the far- and mid-infrared region are used to determine the abundance of methanein Titans lower stratosphere and investigate its distribution with latitude. The CIRS spectra include emissionfrom both the CH4 v4 band at 7.7 micron and pure rotational lines longwards of 50 micron, which show differential sensitivities to thermal profile and methane mole fraction. We analyze nadir and limb data taken over the first part of the Cassini mission (August 2005 to June 2010), including a selection of 12 latitudes that provides a reasonably complete and regular sampling of both hemispheres. Unexpectedly, but in a consistent manner for limb and nadir geometries, large variations of the methane mole fraction near 15 mbar (approx. 85 km) are found, with values ranging from approx. 1.0% (at low latitudes and near +/-50-55 deg) to approx. 1.5% (at +/-30-35 deg and polar latitudes). Error bars on the retrieved methane mole fraction are 0.07-0.12% at low latitudes in the Southern hemisphere and 0.14-0.21% northward of 40 deg N. A 1.0% methanemole fraction at low latitudes permits us to reconcile the HASI-measured temperatures below 147 kmaltitude (2.7 mbar) with inferences from CIRS. The roughly hemispherically-symmetric distribution ofmethane gas is reminiscent of that observed or predicted for the tropospheric methane clouds, whichon a yearly-averaged basis, show preferential occurrences at tropical and polar latitudes. We speculatethat convective events at these latitudes result into local stratospheric methane enrichment, whichmay persist year-round due to dynamical mixing times in the lower stratosphere only moderately shorterthan a Titan year.

Methane↗

Potential Mercurian Analogues: Aubrite and Enstatite Chondrite Impact Melt Meteorites

The MESSENGER (MErcury Surface Space ENvironment GEochemistry and Ranging Spacecraft) mission provided new data that have helped us better constrain the surficial mineralogy and composition of Mercury. Mercury has an extremely low oxygen fugacity (f O2) (Iron Wustite (IW) -7.3 to IW -2.6), and at these unique conditions, elements, which usually exhibit lithophile behavior on Earth, can exhibit chalcophile or siderophile behavior on Mercury. No samples have been returned from Mercury; therefore, we must study candidate meteorite analogs to better understand the formation conditions of minerals inferred to be present at the Mercurian surface and Mercurian magmatic processes. In this study, we present a comprehensive analysis of a representative suite of eight aubrites and four enstatite chondrite impact melts (ECIM), which both have a similar f O2 to Mercury, and contain exotic sulfides that have been inferred to be present at the Mercurian surface. These characteristics allow us to assess their relevance for understanding the mineralogy and magmatic processes of Mercury. The ECIM were previously classified as aubrites, but we show that they are actually ECIM with a potential EH (high enstatite) parent body origin due to the presence of niningerite, Si-enriched kamacite, and uniform Ni in schreibersite. We propose that, with respect to the aubrites, the ECIM represent an ideal candidate for Mercurian studies due to their mineralogy and modal mineralogy. Compared to the aubrites, the ECIM samples do not contain forsterite or diopside, show a poorer sulfide diversity, contain graphite, and have a higher volume percentage of metal phases. Although the Mercurian surface contains forsterite and diopside, graphite and a similar amount of metal and sulfides as seen in the ECIM are inferred to be present on Mercury. According to the calculated normative Mercurian mineralogy, both candidate meteorites are most analogous to the Caloris Basin and Northern Plains Lower Mg regions.

Wilbur, Zoe E.↗

Affordable Development Strategy for NEP Nuclear Systems

One nuclear electric propulsion (NEP) reactor systems under consideration is a hydride moderated thermal spectrum reactor fueled by high assay low enriched uranium (HALEU). While such a reactor is expected to yield the lightest HALEU reactor design, its development challenges grow exponentially with increasing mission demands, most notably power output, specific weight of the overall system (which may require operation at temperatures exceeding 1200 K), service lifetime, and human-rated reliability. Two of the greatest cost drivers are full-powered nuclear demonstrations and extensive material development campaigns, so it is important to consider options that can minimize the need for or complexity of such tasks. This paper discusses a structured framework being developed for assessing how NEP design choices, such as materials selection, neutronic features, and heat-removal technologies, can translate into project risk and how project performance goals can be traded with development cost.Reactors operating at high temperatures often require cutting-edge heat transfer technologies and creep-resistant materials. Use of new materials in high temperature reactors brings additional complication beyond those common to any new space materials development campaign. For example, such materials may not possess necessary neutronic cross-sectional or neutronic irradiation data. Similarly, use of new materials may significantly influence core neutronics; in some extreme cases, neutronic reactivity feed-back of certain new materials can vary during their service life as radiation damage impacts the scattering cross-section. In an affordable development approach, high fidelity modeling and simulation tools are used to identify and characterize potential ‘knees-in-the-curves’ in the relationship that exists between the mission characteristics and the project risk. Of particular significance is use of modern uncertainty management and variance reduction methods to perform gap analyses that feed into phenomena identification and ranking tables (PIRT) commonly used to communicate nuclear readiness levels. Model-based measurements techniques are used to design sub-scale experiments as a substitute to minimize orcompletely eliminate the need for nuclear demonstrations.This paper will describe the approach and present preliminary results. It will lay the groundwork for developing a set of metrics that can be broadly characterized as system nuclear readiness levels and advancement degree of difficulty for nuclear systems. Equally importantly, a goal of this paper is to initiate a dialogue among stakeholders on what is the sufficient level of maturity that is required for launching a demonstration unit.

Dasari V Rao↗

Development of an accelerator-based neutron source to prototype Mo-99 production, Part II: A liquid LBE loop under a high vacuum

To provide US domestic supply of Mo-99 without using high-enriched uranium (HEU), a subcritical uranium target assembly (UTA) is irradiated by an accelerator-based neutron source to create Mo-99 through fission. Part I of this work discusses the design of a liquid lead–bismuth eutectic (LBE) windowless target for an accelerator-based neutron source development. Part II discusses how to couple this windowless target to an accelerator operating at an ultra-high vacuum and the subcritical UTA cooled by water at room temperature. Due to the windowless design of the target, the liquid LBE flow shares an ultra-high vacuum (<1.3 × 10 -7 Pa or 10 -9 Torr) space with the accelerator. As a result of this shared vacuum space, the LBE system must operate at a high vacuum (10 -3 ~10 -6 Pa or 10 -5 ~10 -8 Torr). A magnetic rotary motion feedthrough unit utilizes magnetic fluid to allow rotation of the pump while maintaining a high vacuum environment. Prior to testing the LBE system under vacuum, a pump curve measurement is performed to estimate flowrate in the system. This measurement also generates data on orifice loss coefficients, which are compared to correlations in literature. The second experiment investigates vacuum level in the LBE system during operation. High vacuum is maintained (10 -3 ~10 -5 Pa or 10 -5 ~10 -7 Torr) during system operation, and a residual gas analyzer (RGA) scan shows that partial pressures of residual gases in the LBE system lower over the duration of LBE system operations. The third experiment investigates the gravity driven liquid LBE flowing out of the target chamber in the return line, which is partially full. If the liquid LBE is not drained quickly enough, flooding in the target chamber could occur. The coefficient n in the Manning equation is found to be around 0.008 s/m 1/3 . The last experiment performed is a demonstration that a vacuum jacket could provide sufficient thermal insulation to allow coupling between 300 °C LBE loop and a water tank at room temperature. In conclusion, the results from these experiments have influenced the development of the neutron source for the future commercial scale Mo-99 production system.

43 PARTICLE ACCELERATORS↗

A clustering-based approach to ocean model–data comparison around Antarctica

The Antarctic Continental Shelf seas (ACSS) are a critical, rapidly changing element of the Earth system. Analyses of global-scale general circulation model (GCM) simulations, including those available through the Coupled Model Intercomparison Project, Phase 6 (CMIP6), can help reveal the origins of observed changes and predict the future evolution of the ACSS. However, an evaluation of ACSS hydrography in GCMs is vital: previous CMIP ensembles exhibit substantial mean-state biases (reflecting, for example, misplaced water masses) with a wide inter-model spread. Because the ACSS are also a sparely sampled region, grid-point-based model assessments are of limited value. Our goal is to demonstrate the utility of clustering tools for identifying hydrographic regimes that are common to different source fields (model or data), while allowing for biases in other metrics (e.g., water mass core properties) and shifts in region boundaries. We apply K-means clustering to hydrographic metrics based on the stratification from one GCM (Community Earth System Model version 2; CESM2) and one observation-based product (World Ocean Atlas 2018; WOA), focusing on the Amundsen, Bellingshausen and Ross seas. When applied to WOA temperature and salinity profiles, clustering identifies “primary” and “mixed” regimes that have physically interpretable bases. For example, meltwater-freshened coastal currents in the Amundsen Sea and a region of high-salinity shelf water formation in the southwestern Ross Sea emerge naturally from the algorithm. Both regions also exhibit clearly differentiated inner- and outer-shelf regimes. The same analysis applied to CESM2 demonstrates that, although mean-state model biases in water mass T–S characteristics can be substantial, using a clustering approach highlights that the relative differences between regimes and the locations where each regime dominates are well represented in the model. CESM2 is generally fresher and warmer than WOA and has a limited fresh-water-enriched coastal regimes. Given the sparsity of observations of the ACSS, this technique is a promising tool for the evaluation of a larger model ensemble (e.g., CMIP6) on a circum-Antarctic basis.

54 ENVIRONMENTAL SCIENCES↗

New Perspectives from Satellite and Profile Observations on Tropospheric Ozone over Africa and the Adjacent Oceans: An Indian-Atlantic Ocean Link to tbe "Ozone Paradox"

In the past few years, tropospheric ozone observations of Africa and its adjacent ocenas have been greatly enhanced by high resolution (spatial and temporal) satellite measurements and profile data from aircraft (MOZAIC) and balloon-borne (SHADOZ) soundings. These views have demonstrated for the first time the complexity of chemical-dynamical interactions over the African continent and the Indian and Atlantic Oceans. The tropical Atlantic "ozone paradax" refers to the observation that during the season of maximum biomass burning in west Africa north of the Intertropical Convergence Zone (ITCZ), the highest tropospheric ozone total column occurs south of the ITCZ over the tropical Atlantic. The longitudinal view of tropospheric ozone in the southern tropics from SHADOZ (Southern Hemisphere Additional Ozonesondes) soundings shown the persistence of a "zonal-wave one" pattern that reinforces the "ozone paradox". These ozone features interact with dynamics over southern and northern Africa where anthropogenic sources include the industrial regions of the South African Highveld and Mideastern-Mediterranean influences, respectively. Our newest studies with satellites and soundings show that up to half the ozone pollution over the Atlantic in the January-March "paradox" period may originate from south Asian pollution. Individual patches of pollurion over the Indian Ocean are transported upward by convective mixing and are enriched by pyrogenic, biogenic sources and lightning as they cross Africa and descend over the Atlantic. In summary, local sources, intercontinental import and export and unique regional transport patterns put Africa at a crossroads of troposheric ozone influences.

Thompson, Anne M.↗

Thermodynamic constraints on the textural evolution of eucrite, EET 90020

Basaltic eucrites, which are possibly parts of the Vestan crust, formed as either lava flows or intrusions, and can offer insights into early crust formation. However most eucrites have experienced thermal metamorphism, which exacerbates the challenges of understanding these samples. A variety of petrogenetic models such as, partial melting of a primitive source, fractional crystallization of magmas emplaced in a primitive crust, and/or partial melting of a eucritic source coupled with melt mixing and assimilation have been proposed in order to explain observed petrologic and chemical characteristics of eucrites. EET 90020 is an unbrecciated eucrite that has experienced significant thermal metamorphism, with temperatures of metamorphic equilibration calculated to be ~840-1040°C. Its petrologic history remains contentious, in part due to differences in trace element analyses [10,17, this work], which has led to multiple metamorphic interpretations [8,10-11]. Here, we combined petrologic observations, chemical analyses, and thermodynamic modeling, to interpret micro-domain textures identified in EET 90020 and better constrain its petrologic history. Ultimately, the development of such textures is a direct result of the geologic processes operating on a young Vesta or similar asteroid. Sample description: We identified three textural domains in EET 90020 (Figure 1); (I) A coarse grain domain with granoblastic plagioclase and pyroxene, (II) a medium grain domain with curved grain boundaries between plagioclase and pyroxene, and (III) a fine grain domain dominated by tridymite that fills in interstitial space between spherical plagioclase. Major element chemistry in phases is consistent across domains. Pyroxene have distinctive high Ca lamellae (Wo40.92En28.1Fs31.0) forming from the low-Ca pigeonite host (Wo3.5En33.7Fs62.8). There are small amounts of fayalitic olivine along pyroxene/oxide grain boundaries, where pyroxenes have reacted with ilmenite/chromite clasts. Plagioclase is anorthitic (Avg ~An88), with little variation (An86-92.5). Trace element bulk rock analyses were collected via ICP-MS and individual mineral analyses with LA-ICP-MS (Figure 2). The bulk sample has an observable enrichment in light rare earth elements, with a notable depletion in Eu. Plagioclase is enriched in LREEs, depleted in HREEs, and has a positive Eu anomaly while pyroxene is enriched in HREEs with a negative Eu anomaly. Variations in trace element analyses is likely influenced by the presence of phosphates because are highly enriched in REEs and have a dramatic effect on the bulk domain compositions. Thermodynamic modeling: The software package, Perple_X, was used to calculate mineral phase equilibria over a range of conditions using a Gibbs free energy minimization approach [12]. Models used all major/minor elements except P2O5. Thermodynamic properties from [13] were used to constrain endmember phase stabilities. Activity models were used to describe mixing in phases with solid solution. Isochemical P-T phase diagrams were constructed for the bulk thin section, and the coarse, medium and fine grain domain compositions. Domain compositions were determined using microprobe analyses of phases identified in each domain and observed modal mineral abundancies. Thermodynamically stable pyroxene compositions were extracted from model results and compared to pyroxene compositions collected via EMPA, allowing us to calculate temperatures for metamorphic equilibrium. Results: Results that use the coarse domain composition (Fig. 3a) demonstrate overlap at T ~ 1020°C indicating high T equilibration. There was no overlap for the medium and fine grain domains, or the bulk composition (e.g., Fig. 3b) indicating that these are not equilibrium assemblages. These results were replicated using data from [8,20]. Discussion: The disequilibrium results from the bulk rock, fine grain and medium grain models imply that EET 90020 was modified during and/or after peak metamorphism (open system behavior). However, this is in contrast to the coarse domain where it appears as if equilibrium was maintained. We suggest that this conflict is explained by highly localized equilibrium occurring at millimeter length-scales, which has been observed in terrestrial metamorphism where aqueous fluid infiltrate [14] or melt loss occurs [15]. It is unlikely that aqueous alteration was significant in modifying the bulk composition of EET 90020, because it lacks hydrated minerals. However, the presence of melt is supported by the presence of an abundance of curved grain boundaries and spherical grains in the medium and fine grain domains This contrasts the coarse domain, where grain boundaries are angular. Given the evidence for disequilibrium in the medium and fine grain domains, it would be inappropriate to draw additional conclusions about textural evolution using thermodynamic models of those domains. However, modeling results of the coarse grain domain, where equilibrium was maintained, can provide useful insights into the geologic evolution of EET 90020. We approximate maximum metamorphic temperatures, using the Ca component in pyroxene, at ~1020°C (Fig. 4), which is consistent with previous two-pyroxene thermometry [11]. At this temperature, up to 10 vol % melt can be produced in the coarse grain domain, which is at the boundary of minimum melt needed in order to segregate and form a melt network [16]. Tridymite and plagioclase would be the first minerals to melt out during heating starting ~990°C. This is consistent with the observed melt textures formed by tridymite and plagioclase in the fine grain domain. We suggest that a partial melting model best explains the textural development of EET 90020. Upon heating, melt generated was either trapped, resulting in no net change in the localized rock composition, or migrated, resulting in disequilibrium between pyroxene and the surrounding matrix. A positive Eu anomaly in plagioclase indicates that all of the plagioclase was melted previously. This is consistent with previous studies that have suggested that EET 90020 represents a residual eucrite that experienced partial melting and subsequent melt loss [8]. We speculate that partial melting could have occurred when EET 90020 was heated in the asteroid’s lower crust or adjacent to an intrusion [7]. Future work will study other, texturally heterogeneous samples to see if EET 90020 presents a unique circumstance or whether crustal partial melting was ubiquitous on the eucrite asteroid.

Eucrites↗

Tracing the Evolution of the Cool Gas in CGM and IGM Environments through Mg II Absorption from Redshift z = 0.75 to z = 1.65 Using DESI-Y1 Data

We present a measurement of the mean absorption of cool gas traced by Mg II (λλ2796, 2803) around emission line galaxies (ELGs), spanning spatial scales from 20 kpc to 10 Mpc. The measurement is based on crossmatching the positions ELGs at z = 0.75–1.65 and the metal absorption in the spectra of background quasars with data provided by the Year 1 sample of the Dark Energy Spectroscopic Instrument. The ELGs are divided into two redshift intervals: 0.75 < z < 1.0 and 1.0 < z < 1.65. We find that the composite quasar spectra constructed by stacking the ELG-QSO pairs evolve with redshift, with z > 1 having a systematically higher signal of Mg II absorption. Within 1 Mpc, the covering fraction of the cool gas at z > 1 is higher than that of z < 1. The enhancement becomes less apparent especially if the projected distance r p > 1 Mpc. ELGs with higher stellar mass and star formation rate (SFR) yield higher clustering of Mg ii absorbers at z < 1. For z > 1, the covering fractions with different SFRs show little difference. The higher Mg II absorption at higher redshift supports the observations of higher star formation at cosmic noon. Converting the Mg II absorbers to unsaturated Si II , our estimate indicates that the metal abundance of Si II ranges from 0.7 to 1.2 × 10 −6 from z = 0.9 to 1.3. The growth of low-ionization metal abundance strongly suggests a metal-enriched circumgalactic medium and an increased presence of cool gas in the intergalactic medium toward higher redshifts.

circumgalactic medium↗

Chemical Composition by the APXS along the Downhill Traverse of the Mars Exploration Rover Spirit at Gusev Crater

The Alpha Particle X-ray Spectrometer (APXS) onboard the Mars Exploration Rover Spirit continues to determine the elemental composition of samples at Gusev Crater. Starting around sol 600 the rover descended Husband Hill, which is part of the Columbia Hills, visited the inner basin with a large dune field, called 'El Dorado', and parked at Low Ridge to conserve energy during the martian winter. Many unique samples were discovered by the instruments onboard Spirit during her downhill traverse. Here, we report only on the chemical data obtained by the APXS. The compositions of some of the soil samples are comparable to the mean soil determined along the earlier traverse. However, a light-toned subsurface sample (disturbed by the rover wheels), called Dead Sea Samra , showed the highest sulfur content of all soil samples, the lowest Na, Mg, Al, Cl, K, Ca, Ti, Mn, and Zn, among the lowest Si and P, and among the highest Cr, Fe and Ni. Assuming ferric sulfate as a major mineral, large amounts of a pure silica phase must be present. Color and quantity of Dead Sea Samra resemble somewhat an earlier soil called Paso Robles , though the latter is a mixture of sulfates with phosphate-rich soil. Manganese in Dead Sea Samra is so low that the Fe/Mn ratio exceeds 300, a value that has never been found previously on Mars (Fe/Mn ratio of 46 for Gusev basalts), indicating that only Fe(3+) occurs. The dune field El Dorado contained granulated material that exhibited the highest Mg and Ni concentrations and the lowest S and Cl compared to all other soils implying an enrichment of olivine-rich sands. Two outcrops, called Algonquin and Comanche , revealed compositions that differ significantly from those of earlier outcrops as they have the highest concentrations of Mg, Fe, and Ni (except for Ni in Independence) and the lowest of Al, K (detection limit), Ca, and Ti of all brushed and almost all abraded rocks. Normative estimates assign them the highest olivine contents ever found for martian rocks and a very mafic nature based on their high Mg/(Mg+Fe) and low Al, Ca and Na. Their significantly high Ni contents point to a different source than the Gusev plains basalts. The elemental compositions of samples encountered during the downhill traverse revealed a larger chemical diversity of the Columbia Hills than the uphill trek already published.

Bruckner, J.↗

Initial Delayed Critical Configuration of SHEBA I and Subcritical Measurements by Californium Source-Driven Noise Analysis

In September 1980, the initial delayed critical configuration of the Solution High-Energy Burst Assembly (SHEBA I) was assembled, and the US Department of Energy’s Oak Ridge National Laboratory (ORNL) staff performed near critical and subcritical measurements using the californium source-driven noise analysis (CSDNA) method at the Los Alamos National Laboratory (LANL) Critical Experiments Facility. An unreflected 56 cm outside diameter stainless-steel cylindrical tank was partially filled with uranyl fluoride solution (235U enrichment was 4.95 wt %) until delayed criticality was achieved. Then, measurements were performed for various fuel solution heights from delayed critical to 60% of the height required for delayed criticality. The stainless-steel tank had an inside diameter of 54.6 cm and a height of 105 cm and was partially (20–36.5 cm) filled with an aqueous solution of uranyl fluoride (with a density of 2.162 g/cm 3 ). The density of the uranium in solution was 1.042 g/cm 3 , and the solution had a H/U atomic ratio of 20.43. The tank had a 6.35 mm wall thickness and an axial reentrant tube with an inside diameter of 6.02 cm and wall thickness of 0.165 cm. The tank was essentially unreflected on the top and sides because it was in a thin metal shed. The reactivity of the near–delayed critical configuration was −10.4 cents, which corresponds to a k eff value of 0.99922. In addition to the CSDNA measurements, the prompt neutron decay constants were determined from break frequency noise analysis (BNFA) measurements. The subcritical neutron multiplication factors from CSDNA and BFNA compared extremely well. These data can be used as the basis of International Nuclear Criticality Safety Evaluation benchmark for the near-critical configuration, and the k eff values at various subcritical configurations can be used as subcritical benchmarks. Furthermore, the measured prompt neutron decay constants can be used as reactor physics benchmarks. This report documents the experimental information for the measurements performed so that, at a later date, researchers could perform the required uncertainty and calculational analyses and documentation to use these data for an International Criticality Safety Benchmark Evaluation Program (ICSBEP) or Nuclear Energy Agency benchmarks. The measured prompt neutron decay constants can be used as the basis of a benchmark for the International Reactor Physics Evaluation Program (IRPhEP). The data from these measurements are available from the ORNL Records Management Services Department, and the logbook is available from ICSBEP at Idaho National Laboratory.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Enhanced Resistance Pines for Improved Renewable Biofuel and Chemical Production (Technical Report)

We completed phenotyping constitutive and inducible oleoresin flow across two seasons, constitutive resin canal number and density and wood terpene content in our ADEPT2 and CCLONES populations. We completed genetic association between 19 oleoresin phenotypes and a total of 523,192 SNP markers from ADEPT2 and 13,883 SNP markers in CCLONES using four mixed linear models. A total of 293 significant SNPs (FDR = 0.20) were identified. We used the MENTOR tool to mine mechanistic connections from a multiplex network constructed from poplar multi-omic data to construct a conceptual model for a subset of these significant SNPs. Our model contains 6 transcriptional regulators in addition to 3 monoterpene synthases. To generate more lines of evidence for these significant SNPs, we completed a time course RNAseq experiment after inducing vascular zone cells to differentiate into new resin canals with a methyl jasmonate treatment, a single nuclei RNAseq that identified differentiating resin canal epithelial cells and are completing analysis for a QTL study in a hybrid pine population. The time course identified 4634 significantly down and 1890 significantly up regulated transcripts after treatment with methyl jasmonate, an inducer of new resin canal formation in the vascular cambial meristem. To analyze this large set of differentially regulated genes, we created a predictive expression network and analyzed it with random walk restart using 6 seed genes coding for transcription factors regulating xylem differentiation in poplar. Of the top ranked 200 transcripts, 119 transcripts were significant differentially expressed supporting these transcripts as potential candidates regulating resin canal formation. Analysis of single nuclei sequencing of shoot tips that contain differentiating resin canals, identified 10 clusters. One cluster was highly enriched in transcripts coding for 9 of the enzymes in the MEP pathway 3 prenyl synthetases, and 3 monoterpene synthases strongly suggesting that this cluster represents resin canal epithelial cells. We are mining the additional transcripts to create a trajectory analysis. In summary, we have identified > 10 novel genes that are strongly supported candidates for further analysis in breeding lines and for genetic engineering over- and under- expressing lines to increase wood terpene content to improve resistance to insect and fungal pathogens while simultaneously increasing terpene supplies for renewable chemicals and biofuels.

59 BASIC BIOLOGICAL SCIENCES↗

A Gene Expression and Histologic Approach to Study Production and Outflow of Cerebrospinal Fluid in Hindlimb Suspended Rats

INTRODUCTION: The Spaceflight Associated Neuro-ocular Syndrome (SANS) is thought to be associated with weightlessness-induced cephalad fluid shift, possibly associated with a chronic elevation of intracranial pressure (ICP) during long-duration ISS missions. Changes in cerebrospinal fluid (CSF) dynamics and cranial compliance might be involved in the ICP increase. It is not known whether CSF production and/or outflow are altered in microgravity, but changes at the molecular and cellular level in the structures that produce and regulate the transcellular and paracellular secretion and reabsorption of CSF may be relevant. In this study, we used the rat hindlimb suspension (HS) model to examine the relationship between intracranial pressure (ICP) and the cellular responses to the prolonged change in body posture elicited specifically in the choroid plexus (CP). This was evaluated by transcriptomics, histopathology and ultrastructure of the CP and arachnoid villi (AV). METHODS: ICP was measured by telemetry. The morphology, ultrastructure, and gene expression profile of the CP was examined using male 9-month-old Long Evans rats subjected to HS for 14 and 90 days. A subset of animals completing 90-day HS returned to normal posture for 14 and 90 additional days of recovery. All HS rats had age-matched cage controls maintained in normal posture. A group of animals was maintained in conditions of 1% CO2 throughout the entire protocol. The rat brains were carefully removed and preserved for various analyses, including transmission electron microscopy (TEM), immunohistochemical analysis of specific targets involved in CSF regulation, and RNA sequencing analysis of laser capture micro-dissected CP tissue from the lateral ventricles. SUMMARY OF RESULTS: The ICP record was limited, which impacted any conclusions derived from the results. However, the ICP of rats in the CO2-enriched atmosphere appeared increased compared to those in a normal air composition. HS of rats resulted in an altered transcriptomics profile in the choroid plexus compared to animals maintained at normal posture. This was observed during both the HS period and during normal posture recovery period following 90 days of suspension. The greatest number of differentially expressed genes was observed at 90 days HS. Elevated CO2 also led to a different transcriptomics profile in the CP. The histology and ultrastructure results should be considered preliminary due to the limited number of samples. Examination of the CP by TEM showed blood vessel congestion and microvilli swelling in the CP, as well as some subpial and periventricular gliosis, but no associations were observed with treatment. There was no evidence of a reduced clearance of b-amyloid in the periventricular, perivascular and subpial regions of the brain in HS animals versus those in normal posture. Immunohistochemical staining of aquaporin 4, showed the localization to the subpial region and ependyma with increased immunoreactivity in the brains of CO2 exposed rats. This work was supported by awards NNX15AW48G to S.Z. Animal tissue and live data was provided under a tissue/data sharing agreement with Dr. Charles Fuller, UC Davis.

S B Zanello↗

A Gene Expression and Histologic Approach to Study Production and Outflow of Cerebrospinal Fluid in Hindlimb Suspended RATS

INTRODUCTION: The Spaceflight Associated Neuro-ocular Syndrome (SANS) is thought to be associated with weightlessness-induced cephalad fluid shift, possibly associated with a chronic elevation of intracranial pressure (ICP) during long-duration ISS missions. Changes in cerebrospinal fluid (CSF) dynamics and cranial compliance might be involved in the ICP increase. It is not known whether CSF production and/or outflow are altered in microgravity, but changes at the molecular and cellular level in the structures that produce and regulate the transcellular and paracellular secretion and reabsorption of CSF may be relevant. In this study, we used the rat hindlimb suspension (HS) model to examine the relationship between intracranial pressure (ICP) and the cellular responses to the prolonged change in body posture elicited specifically in the choroid plexus (CP). This was evaluated by transcriptomics, histopathology and ultrastructure of the CP and arachnoid villi (AV). METHODS: ICP was measured by telemetry. The morphology, ultrastructure, and gene expression profile of the CP was examined using male 9-month-old Long Evans rats subjected to HS for 14 and 90 days. A subset of animals completing 90-day HS returned to normal posture for 14 and 90 additional days of recovery. All HS rats had age-matched cage controls maintained in normal posture. A group of animals was maintained in conditions of 1% CO2 throughout the entire protocol. The rat brains were carefully removed and preserved for various analyses, including transmission electron microscopy (TEM), immunohistochemical analysis of specific targets involved in CSF regulation, and RNA sequencing analysis of laser capture micro-dissected CP tissue from the lateral ventricles. SUMMARY OF RESULTS: The ICP record was limited, which impacted any conclusions derived from the results. However, the ICP of rats in the CO2-enriched atmosphere appeared increased compared to those in a normal air composition. HS of rats resulted in an altered transcriptomics profile in the choroid plexus compared to animals maintained at normal posture. This was observed during both the HS period and during normal posture recovery period following 90 days of suspension. The greatest number of differentially expressed genes was observed at 90 days HS. Elevated CO2 also led to a different transcriptomics profile in the CP. The histology and ultrastructure results should be considered preliminary due to the limited number of samples. Examination of the CP by TEM showed blood vessel congestion and microvilli swelling in the CP, as well as some subpial and periventricular gliosis, but no associations were observed with treatment. There was no evidence of a reduced clearance of b-amyloid in the periventricular, perivascular and subpial regions of the brain in HS animals versus those in normal posture. Immunohistochemical staining of aquaporin 4, showed the localization to the subpial region and ependyma with increased immunoreactivity in the brains of CO2 exposed rats. This work was supported by awards NNX15AW48G to S.Z. Animal tissue and live data was provided under a tissue/data sharing agreement with Dr. Charles Fuller, UC Davis.

S B Zanello↗

Abundance Measurements of Titan's Stratospheric HCN, HC3N, C3H4, and CH3CN from ALMA Observations

Previous investigations have employed more than 100 close observations of Titan by the Cassini orbiter to elucidate connections between the production and distribution of Titan's vast, organic-rich chemical inventory and its atmospheric dynamics. However, as Titan transitions into northern summer, the lack of incoming data from the Cassini orbiter presents a potential barrier to the continued study of seasonal changes in Titan's atmosphere. In our previous work, we demonstrated that the Atacama Large Millimeter/submillimeter Array (ALMA) is well suited for measurements of Titan's atmosphere in the stratosphere and lower mesosphere (~100 - 500 km) through the use of spatially resolved (beam sizes <1") flux calibration observations of Titan. Here, we derive vertical abundance profiles of four of Titan's trace atmospheric species from the same 3 independent spatial regions across Titan's disk during the same epoch (2012-2015): HCN, HC3N, C3H4, and CH3CN. We find that Titan's minor constituents exhibit large latitudinal variations, with enhanced abundances at high latitudes compared to equatorial measurements; this includes CH3CN, which eluded previous detection by Cassini in the stratosphere, and thus spatially resolved abundance measurements were unattainable. Even over the short 3-year period, vertical profiles and integrated emission maps of these molecules allow us to observe temporal changes in Titan's atmospheric circulation during northern spring. Our derived abundance profiles are comparable to contemporary measurements from Cassini infrared observations, and we find additional evidence for subsidence of enriched air onto Titan's south pole during this time period. Continued observations of Titan with ALMA beyond the summer solstice will enable further study of how Titan's atmospheric composition and dynamics respond to seasonal changes.

Thelen, Alexander E.↗