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At least 1,369 records · Page 76

Status of Brayton Cycle Power Conversion Development at NASA GRC

The NASA Glenn Research Center (GRC) is pursuing the development of Brayton cycle power conversion for various NASA initiatives. Brayton cycle power systems offer numerous advantages for space power generation including high efficiency, long life, high maturity, and broad scalability. Candidate mission applications include surface rovers and bases, advanced propulsion vehicles, and earth orbiting satellites. A key advantage is the ability for Brayton converters to span the wide range of power demands of future missions from several kilowatts to multi-megawatts using either solar, isotope, or reactor heat sources. Brayton technology has been under development by NASA since the early 1960's resulting in engine prototypes in the 2 to 15 kW-class that have demonstrated conversion efficiency of almost 30% and cumulative operation in excess of 40,000 hours. Present efforts at GRC are focusing on a 2 kW testbed as a proving ground for future component advances and operational strategies, and a 25 kW engine design as a modular building block for 100 kW-class electric propulsion and Mars surface power applications.

Mason, Lee S.↗

Geographic Information Systems and Web Page Development

The Facilities Engineering and Architectural Branch is responsible for the design and maintenance of buildings, laboratories, and civil structures. In order to improve efficiency and quality, the FEAB has dedicated itself to establishing a data infrastructure based on Geographic Information Systems, GIS. The value of GIS was explained in an article dating back to 1980 entitled "Need for a Multipurpose Cadastre" which stated, "There is a critical need for a better land-information system in the United States to improve land-conveyance procedures, furnish a basis for equitable taxation, and provide much-needed information for resource management and environmental planning." Scientists and engineers both point to GIS as the solution. What is GIS? According to most text books, Geographic Information Systems is a class of software that stores, manages, and analyzes mapable features on, above, or below the surface of the earth. GIS software is basically database management software to the management of spatial data and information. Simply put, Geographic Information Systems manage, analyze, chart, graph, and map spatial information. GIS can be broken down into two main categories, urban GIS and natural resource GIS. Further still, natural resource GIS can be broken down into six sub-categories, agriculture, forestry, wildlife, catchment management, archaeology, and geology/mining. Agriculture GIS has several applications, such as agricultural capability analysis, land conservation, market analysis, or whole farming planning. Forestry GIs can be used for timber assessment and management, harvest scheduling and planning, environmental impact assessment, and pest management. GIS when used in wildlife applications enables the user to assess and manage habitats, identify and track endangered and rare species, and monitor impact assessment.

Reynolds, Justin↗

Geographic Information Systems and Web Page Development

The Facilities Engineering and Architectural Branch is responsible for the design and maintenance of buildings, laboratories, and civil structures. In order to improve efficiency and quality, the FEAB has dedicated itself to establishing a data infrastructure based on Geographic Information Systems, GIs. The value of GIS was explained in an article dating back to 1980 entitled "Need for a Multipurpose Cadastre which stated, "There is a critical need for a better land-information system in the United States to improve land-conveyance procedures, furnish a basis for equitable taxation, and provide much-needed information for resource management and environmental planning." Scientists and engineers both point to GIS as the solution. What is GIS? According to most text books, Geographic Information Systems is a class of software that stores, manages, and analyzes mapable features on, above, or below the surface of the earth. GIS software is basically database management software to the management of spatial data and information. Simply put, Geographic Information Systems manage, analyze, chart, graph, and map spatial information. At the outset, I was given goals and expectations from my branch and from my mentor with regards to the further implementation of GIs. Those goals are as follows: (1) Continue the development of GIS for the underground structures. (2) Extract and export annotated data from AutoCAD drawing files and construct a database (to serve as a prototype for future work). (3) Examine existing underground record drawings to determine existing and non-existing underground tanks. Once this data was collected and analyzed, I set out on the task of creating a user-friendly database that could be assessed by all members of the branch. It was important that the database be built using programs that most employees already possess, ruling out most AutoCAD-based viewers. Therefore, I set out to create an Access database that translated onto the web using Internet Explorer as the foundation. After some programming, it was possible to view AutoCAD files and other GIS-related applications on Internet Explorer, while providing the user with a variety of editing commands and setting options. I was also given the task of launching a divisional website using Macromedia Flash and other web- development programs.

Reynolds, Justin↗

X2000 power system electronics development

The X2000 Power System Electronics (PSE) is a Jet Propulsion Laboratory (JPL) task to develop a new generation of power system building blocks for potential use on future deep space missions. The effort includes the development of electronic components and modules that can be used as building blocks in the design of generic spacecraft power systems.

X2000↗

Power Actuation and Switching Module Development

The Deep Space Avionics (DSA) Project is developing a Power Actuation and Switching Module (PASM). This component enables a modular and scalable design approach for power switching applications, which can result in a wide variety of power switching architectures using this simple building block. The PASM is designed to provide most of the necessary power switching functions of spacecraft for various Deep Space missions including future missions to Mars, comets, Jupiter and its moons. It is fabricated using an A SIC process that is tolerant of high radiation. The development includes two application specific integrated circuits (ASICs) and support circuitry all packaged using High Density Interconnect (HDI) technology. It can be operated in series or parallel with other PASMs, It can be used as a high-side or low-side switch and it can drive thruster valves, pyrotechnic devices such as NASA standard initiators, bus shunt resistors, and regular spacecraft component loads. Each PASM contains two independent switches with internal current limiting and over-current trip-off functions to protect the power subsystem from load faults. During turnon and turnoff each switch can limit the rate of current change (di/dt) to a value determined by the user. Threeway majority-voted On/Off commandability and full switch status telemetry (both analog and digital) are built into the module. This paper describes the development process used to design, model, fabricate, and test these compact and versatile power switches. Preliminary test results from prototype HDI PASM hardware are also discussed.

Power Actuation and Switching Module (PASM)↗

Project Interface Requirements Process Including Shuttle Lessons Learned

Most failures occur at interfaces between organizations and hardware. Processing interface requirements at the start of a project life cycle will reduce the likelihood of costly interface changes/failures later. This can be done by adding Interface Control Documents (ICDs) to the Project top level drawing tree, providing technical direction to the Projects for interface requirements, and by funding the interface requirements function directly from the Project Manager's office. The interface requirements function within the Project Systems Engineering and Integration (SE&I) Office would work in-line with the project element design engineers early in the life cycle to enhance communications and negotiate technical issues between the elements. This function would work as the technical arm of the Project Manager to help ensure that the Project cost, schedule, and risk objectives can be met during the Life Cycle. Some ICD Lessons Learned during the Space Shuttle Program (SSP) Life Cycle will include the use of hardware interface photos in the ICD, progressive life cycle design certification by analysis, test, & operations experience, assigning interface design engineers to Element Interface (EI) and Project technical panels, and linking interface design drawings with project build drawings

Bauch, Garland T.↗

NASA Ares I Launch Vehicle Upper Stage Reaction Control System (ReCS) Cold Flow Development Test Overview

NASA s Ares I launch vehicle, consisting of a five segment solid rocket booster first stage and a liquid bi-propellant J2-X engine Upper Stage, is the vehicle that s been chosen to launch the Orion Crew Module, which will return humans to the Moon, Mars, and beyond. After First Stage booster separation, the Reaction Control System (ReCS), a monopropellant hydrazine system, will provide the Upper Stage element with three degrees of freedom control as needed. This paper provides an overview of the system level development testing that has taken place on the Ares I launch vehicle Upper Stage ReCS. The ReCS System Development Test Article (SDTA) was built as a flight representative water flow test article whose primary test objective was to obtain fluid system performance data to evaluate the integrate system performance characteristics and verify analytical models. Water is the industry standard for cold flow testing of hydrazine systems, because the densities are very close and the speeds of sound are well characterized. The completion of this development level test program was considered necessary to support the ReCS Critical Design Review. This paper will address the design approach taken in building the test article, the objectives of the test program, types of testing completed, general results, the ability of the program to meet the test objectives, and lessons learned

Dervan, Melanie↗

Heading Toward Launch with the Integrated Multi-Satellite Retrievals for GPM (IMERG)

The Day-l algorithm for computing combined precipitation estimates in GPM is the Integrated Multi-satellitE Retrievals for GPM (IMERG). We plan for the period of record to encompass both the TRMM and GPM eras, and the coverage to extend to fully global as experience is gained in the difficult high-latitude environment. IMERG is being developed as a unified U.S. algorithm that takes advantage of strengths in the three groups that are contributing expertise: 1) the TRMM Multi-satellite Precipitation Analysis (TMPA), which addresses inter-satellite calibration of precipitation estimates and monthly scale combination of satellite and gauge analyses; 2) the CPC Morphing algorithm with Kalman Filtering (KF-CMORPH), which provides quality-weighted time interpolation of precipitation patterns following cloud motion; and 3) the Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks using a Cloud Classification System (PERSIANN-CCS), which provides a neural-network-based scheme for generating microwave-calibrated precipitation estimates from geosynchronous infrared brightness temperatures. In this talk we summarize the major building blocks and important design issues driven by user needs and practical data issues. One concept being pioneered by the IMERG team is that the code system should produce estimates for the same time period but at different latencies to support the requirements of different groups of users. Another user requirement is that all these runs must be reprocessed as new IMERG versions are introduced. IMERG's status at meeting time will be summarized, and the processing scenario in the transition from TRMM to GPM will be laid out. Initially, IMERG will be run with TRMM-based calibration, and then a conversion to a GPM-based calibration will be employed after the GPM sensor products are validated. A complete reprocessing will be computed, which will complete the transition from TMPA.

Huffman, George J.↗

Simulation of Image Performance Characteristics of the Landsat Data Continuity Mission (LDCM) Thermal Infrared Sensor (TIRS)

The next Landsat satellite, which is scheduled for launch in early 2013, will carry two instruments: the Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS). Significant design changes over previous Landsat instruments have been made to these sensors to potentially enhance the quality of Landsat image data. TIRS, which is the focus of this study, is a dual-band instrument that uses a push-broom style architecture to collect data. To help understand the impact of design trades during instrument build, an effort was initiated to model TIRS imagery. The Digital Imaging and Remote Sensing Image Generation (DIRSIG) tool was used to produce synthetic "on-orbit" TIRS data with detailed radiometric, geometric, and digital image characteristics. This work presents several studies that used DIRSIG simulated TIRS data to test the impact of engineering performance data on image quality in an effort to determine if the image data meet specifications or, in the event that they do not, to determine if the resulting image data are still acceptable.

Landsat↗

Air Traffic Management Technology Demonstration-1 (ATD-1) Avionics Phase 2 Flight Test Training for Interval Management

Prior to the successful flight test validation of a new avionics prototype, participants from Boeing, Honeywell, and United Airlines underwent group training at NASA Langley Research Center. New prototype software for an algorithm which enables greater efficiency in high-density airspace, called Interval Management, was to be incorporated into Electronic Flight Bags and placed in the cockpit for pilot usage. The goals of the training were to teach the flight test pilots how to operate the new software, establish techniques to simultaneously position three aircraft prior to each test scenario, and ensure a common communication protocol among team members when coordinating the position of aircraft for the next scenario. The multi-tiered interactive training regimen consisted of a process that continually built upon previous foundational material. The primary learning elements were 1) a portable computer-based trainer that was provided to the pilots prior to classroom training sessions, 2) classroom learning, 3) full mock-up simulator training, and 4) refresher training just prior to the flight test. Each part of the regimen was designed to repeat and build upon the previous element. The purpose of this Technical Memorandum is to inform the aviation industry how flight training for Interval Management was conducted at Langley Research Center in order to reduce overall development costs of future Interval Management training programs. Secondly, the paper provides insight regarding the decision-making process when attempting to conduct a flight test.

Roper, Roy D.↗

Strategic Deconfliction Performance: Results and Analysis from the NASA UTM Technical Capability Level 4 Demonstration

Unmanned Aircraft System (UAS) Traffic Management (UTM) refers to the service-based, cooperative approach to the management of small UAS in the National Airspace System that is safe, scalable, and fair. UTM provides the means to manage the airspace in a complementary manner that does not burden the current air traffic control workforce or infrastructure but allows the Air Navigation Service Provider to maintain its regulatory and operational authority of the airspace. A key feature of UTM is the ability to provide operators the means to strategically deconflict operations from others in the airspace through the digital exchange of information via supporting services. Through this approach, the four-dimensional operation volumes that encompass the intent of operators in a given area are discoverable and can be used for airspace awareness as well as planning conflict free operations that account for and avoid other operations. In certain cases, it is also possible to negotiate volume intersections for shared airspace use without the need to re-plan. In the NASA UTM concept, strategic deconfliction is the first layer of three in the overall conflict management model. The three layers of the conflict management model, which follow the International Civil Aviation Organization’s scheme [ICAO 2005] are: strategic conflict management, separate provision, and collision avoidance. In UTM, the strategic layer mostly occurs prior to departure, but is applicable to en route operations with sufficient planning horizon. The initial requirements for a strategic deconfliction capability within UTM are defined in a NASA publication [Rios 2018]. Within the concept and implementation of service-provided strategic deconfliction is the notion of priority. It is understood that there are instances in which an operation requires a priority designation within the UTM system and special handling accordingly to provide situation awareness and facilitate appropriate responses from other airspace users. Examples of situations requiring priority designation include: when an operator declares an emergency due to problems with the vehicle or its immediate surroundings; operations that are in support of certain organizations (e.g., public safety and first responders); or special missions that also require priority use of airspace (e.g., emergency medical deliveries). UAS Volume Reservations (UVRs) also relate to the topic of priority in the sense that the airspace that the volume encompasses has a different status or classification in which unassociated operations must vacate if inside, or avoid if outside, through strategic deconfliction with the volume. Operations that are specially permitted to access the UVR area are typically assigned priority status given the nature of their mission and their associated credentials. The ability to perform strategic deconfliction, handle certain operations with a priority distinction, and establish UVRs that are communicated throughout the UTM system, is predicated on an architecture that has been established through an evolutionary process in response to close collaboration with stakeholders from government and industry. Another important and influential aspect of these capabilities and architecture is the live, distributed flight tests that have been conducted across the Technical Capability Levels (TCLs) that culminated with a set of complex tests performed as part of TCL4 [Rios 2020]. The TCL4 flight test involved two FAA-designated UAS test sites building teams to collaborate with NASA’s UTM Project on the execution of several detailed, small UAS scenarios in urban environments.

conflict management↗

Cognitive Tapered Slot Circular Array Antenna for Lunar Surface Communications

In this paper, we present the design of a cognitive tapered slot circular array (TSCA) antenna with multiple electronically switched sector beams for lunar surface communication. The circular array is capable of sensing the frequency, power, and direction of arrival of the RF signals. The design of the TSCA builds on our prior work on the development of a single TSA element with a balanced microstrip/coax feed. The measured return loss of the single TSA element shows wide bandwidth with good impedance match across the 5 to 35 GHz frequency range. Additionally, the single TSA has good radiation patterns. The single TSA serves as the building block for a four-element TSCA with multiple electronically switched sector beams for sensing and communications.

Cognitive communications↗

Cognitive Tapered Slot Circular Array Antenna for Lunar Surface Communications

In this paper, we present the design of a cognitive tapered slot circular array (TSCA) antenna with multiple electronically switched sector beams for lunar surface communication. The circular array is capable of sensing the frequency, power, and direction of arrival of the RF signals. The design of the TSCA builds on our prior work on the development of a single TSA element with a balanced microstrip/coax feed. The measured return loss of the single TSA element shows wide bandwidth with good impedance match across the 5 to 35 GHz frequency range. Additionally, the single TSA has good radiation patterns. The single TSA serves as the building block for a four-element TSCA with multiple electronically switched sector beams for sensing and communications.

Antenna↗

Development of an Ultra High-Pressure Metals Promoted Combustion in Oxygen Test Apparatus

Materials flammability data is needed at pressures higher than ever tested before (greater than 10,000 psi). WSTF has designed and is currently building a metals flammability apparatus that can test metals up to 30,000 psi. Solid progress was achieved on the chamber in terms of system build up and fulfilling requirements to perform a NASA Test Readiness Review. Understanding metals flammability data at extreme pressure opens the doors for higher pressure and higher performance oxygen systems. Proposed systems include more reliable/higher performance propulsion systems and more reliable/lighter Environmental Control Life Support Systems (ECLSS). The test system needed for this testing required significant research and development to select a pressurization method and eliminate contact with potentially flammable materials back to the chamber. No additional promoted combustion data was collected in FY21. This International Research & Development (IRAD) proposal was critical to getting the system very close to operational status. This capability is critical to collect forward leaning material flammability data for future spacecraft and ground systems. Forward work has been identified and funding is being sought outside of the Center Innovation Fund (CIF) process for late FY23/FY24.

promoted combustion↗

Autonomous Ocean World Exploration: Advancement of a Virtual Testbed

The search for life (extinct or extant) and potentially habitable bodies in our solar system and beyond is one of the 12 priority science questions outlined in the National Acadamies’ 2022 decadal survey [5]. Extraterrestrial destinations containing liquid water present an opportunity to search for life as we know it, and in recent years an increasing number of such locations have been discovered within our solar system. Several Jovian moons—Europa, Ganymede, and Callisto [10]—and the Saturnian moons Enceladus [8] and Titan [9] are known or suspected to harbor massive subsurface oceans. Of these "ocean worlds", Europa is the focus of at least one planned NASA orbiter mission, Europa Clipper [4], and an early lander mission concept, the Europa Lander [2, 3]. Whereas most robotic missions to the Moon and Mars (e.g. orbiters, rovers, landers) to date have had ground controllers on Earth tightly involved in mission operations, missions to more distant worlds will require a high degree of onboard autonomy due to long communication lags and blackouts, harsh environments (radiation, cold), and more limited battery and hardware life. The past decade has seen great advances in both AI technologies and computing scalability and performance that offer promising solutions for spacecraft autonomy and motivate the software system and research programs described in this paper. The Ocean Worlds Autonomy Testbed for Exploration, Research, and Simulation (OceanWATERS) [1], which has been in development at the NASA Ames Research Center since 2018, is a virtual environment for testing lander autonomy solutions. It is built on the Robot Operating System (ROS), runs on consumer-grade Linux workstations, and was released as open source in 2020. OceanWATERS provides a physical and visual simulation of a prototypical lander in a Europa-like environment (Figure 1). The lander was modeled after requirements and specifications made in JPL’s Europa Lander Study of 2016 [3]. Simulated lander systems include stereo cameras and spotlights mounted on an antenna mast that pans and tilts, a 6 degrees of freedom (DoF) robotic arm with a force-torque sensor and two interchangeable end effectors, and a battery pack power system. The environment consists of multiple terrain models including a highly detailed model sourced from the FROST dataset [11], simulation of surrounding planetary bodies based on an ephemeris model, and lighting from the sun with associated surface illumination, reflectance, and shadows. Operations supported by OceanWATERS include panoramic and directed imaging of the environment and lander workspace, Cartesian and joint-level arm commanding, grinding of the terrain surface (e.g. digging a trench), and scooping of ground material (Figure 2) which can be discarded or collected as science samples in a receptacle that can be emptied (science operations themselves are not simulated). These operations are realized as ROS Actions and are complimented by a wide selection of telemetry that is continually produced by each lander subsystem. The power system model is driven by the open-source Generic Software Architecture for Prognostics (GSAP) [11] that predicts the battery’s remaining useful life and other characteristics. As a testbed for high-level autonomy, OceanWATERS provides an execution framework based on PLEXIL [12], an open-source plan specification language and execution engine developed largely at Ames. NASA's initial development of OceanWATERS, as well the Ocean Worlds Lander Autonomy Testbed (OWLAT) [6], a complimentary physical testbed developed at JPL, was the first step in a plan for realizing candidate onboard autonomy solutions for such planetary landers. In 2020 NASA solicited applications for its Autonomous Robotics Research for Ocean Worlds (ARROW) program, and in 2021 the similar Concepts for Ocean worlds Life Detection Technology (COLDTech) program. Collectively six research teams, based in universities and companies across the United States, were awarded grants to develop and demonstrate autonomy solutions on OceanWATERS and OWLAT. These 1–2-year projects have now finished or are nearing completion, and a wide variety of autonomy challenges in ocean world surface missions were addressed. Prototyped and demonstrated solutions have included autonomous discovery, response and adaptation to system faults and unexpected environmental events, world model synthesis through perception, plan synthesis using learned models, methods to optimize sample target selection and prioritize science data transmission, extension of PLEXIL for stochastic decision-making, and an integration of a model of JPL’s mission-ready COLDArm [7]. Technologies used in these projects include many forms of machine learning, causal reasoning, automated planning, Markov decision processes, formal methods, and other advanced techniques. A more detailed summary of the ARROW and COLDTech projects is given herein. OceanWATERS has had significant enhancements since its open-source release in 2020. Many of its new features were driven or shaped by feedback from the ARROW and COLDTech teams and requirements of their projects. In support of enabling autonomous adaptation to spacecraft faults (a specific capability solicited by both programs), a fault injection and detection framework was developed that supports a wide and growing range of fault types such as locked joints, image loss, and battery failures. The power system model was completed and integrated into the simulator, starting as a single-cell battery model and later upgraded to a multi-cell model with associated faults such as cell disconnection. Arm/terrain interaction was improved by adding a force-torque sensor and associated faults, and an analytic dig force model based on the Balovnev bucket force equations. Environment fidelity was increased by modeling terrain deformation resulting from digging and scooping; visual improvements were made in textures, lighting, and shadows. To facilitate interoperation with OWLAT, a unified command and telemetry interface between the testbeds was developed at the ROS level, along with a PLEXIL interface. The number of lander operations was greatly expanded (e.g. with Cartesian-based arm and antenna movement), and a framework was designed for users to build their own lander actions. A GUI for PLEXIL plan selection was created (Figure 3), and an expansive set of plans were added, such as those that illustrate patterns for fault handling. This paper provides a self-contained high-level description of OceanWATERS, focusing on more detailed coverage of the aforementioned enhancements. It provides a high-level summary of the projects undertaken by participants in the ARROW and COLDTech programs and how these efforts have helped shape OceanWATERS. Finally, potential future work and directions for the testbed are listed, as likely informed by the recent planetary science decadal survey [5].

K Michael Dalal↗

Foreword 1: The Medici Effect and the Importance of Women in Manufacturing

In 2004, Frans Johansson published “The Medici Effect” which emphasizes the need for diversity for innovation. His hypothesis is based on the Medici Dynasty in the 14th Century and their role in the birth of the Renaissance. The Medici’s financial support of painters, poets, scientists, architects… a variety of disciplines working closely together on diverse problems, led to an explosion of innovations. We still see this today in pockets of industries where architects work with entomologists on new designs of energy efficient building, roboticist study nature to understand locomotion and manipulation. One of my most rewarding programs was when I worked with a microbiologist to use bacteria to synthesize magnetic nanoparticles for ferrofluids. Diversity is the fuel for innovation. Our greatest source of fuel for diversity is gender.

Love, Lonnie↗

A hybrid method to evaluate the life cycle climate performance of heat pumps

Heat pumps have a significant impact on the climate, and numerous studies exist discussing the environmental impact of heat pumps quantitatively. Life Cycle Climate Performance (LCCP) is a widely accepted metric and can evaluate the carbon footprint of heat pumps from cradle to grave. LCCP calculation typically requires inputs like annual energy consumption, the material used, refrigerant type, and charge level, which are usually the intermediate or final results from heat pump simulation tools. Thus, a platform linking the heat pump simulation software, local weather conditions, and LCCP evaluation tool can simplify the heat pump design process, especially for complicated systems. This paper presents an LCCP evaluation tool accessing the outputs from the DOE Heat Pump Design Model and the building loads and temperature bin data from AHRI standard 210/240. As for case studies, this paper investigates the LCCP values and life cycle electricity costs (LCEC) of a given cooling-based heat pump with R410A in five typical US cities. In conclusion, this cooling-based heat pump has the highest LCCP value at Chicago and lowest LCCP value at Seattle. In contrast, the heat pump has the highest LCEC at Seattle and the lowest LCEC at Miami. As a future work, a more comprehensive carbon emission and cost analysis of heat pump systems will be conducted through all major cities around the world.

Shen, Bo↗

Engineering Crassulacean Acid Metabolism in C3 and C4 Plants

Carbon dioxide (CO2) is a major greenhouse gas contributing to changing climatic conditions, which is a grand challenge affecting the security of food, energy, and environment. Photosynthesis plays the central role in plant-based CO2 reduction. Plants performing CAM (crassulacean acid metabolism) photosynthesis have a much higher water use efficiency than those performing C3 or C4 photosynthesis. Therefore, there is a great potential for engineering CAM in C3 or C4 crops to enhance food/biomass production and carbon sequestration on arid, semiarid, abandoned, or marginal lands. Recent progresses in CAM plant genomics and evolution research, along with new advances in plant biotechnology, have provided a solid foundation for bioengineering to convert C3/C4 plants into CAM plants. Here, we first discuss the potential strategies for CAM engineering based on our current understanding of CAM evolution. Then we describe the technical approaches for engineering CAM in C3 and C4 plants, with a focus on an iterative four-step pipeline: (1) designing gene modules, (2) building the gene modules and transforming them into target plants, (3) testing the engineered plants through an integration of molecular biology, biochemistry, metabolism, and physiological approaches, and (4) learning to inform the next round of CAM engineering. Finally, we discuss the challenges and future opportunities for fully realizing the potential of CAM engineering.

Yang, Xiaohan↗