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At least 1,369 records · Page 76
All sourcesNASA NTRSDOE OSTI
DOE OSTI2012

QB1 - Stochastic Gene Regulation

Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present in the cell - Random motion and competition between reactants, Low copy, quantization of reactants, Upstream processes; (2) Fluctuations may be very important - Cell-to-cell variability, Cell fate decisions (switches), Signal amplification or damping, stochastic resonances; and (3) Some tools are available to mode these - Kinetic Monte Carlo simulations (SSA and variants), Moment approximation methods, Finite State Projection. We will see how modeling these reactions can tell us more about the underlying processes of gene regulation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2012

Development and validation of a citrate synthase directed quantitative PCR marker for soil bacterial communities

Molecular innovations in microbial ecology are allowing scientists to correlate microbial community characteristics to a variety of ecosystem functions. However, to date the majority of soil microbial ecology studies target phylogenetic rRNA markers, while a smaller number target functional markers linked to soil processes. We validated a new primer set targeting citrate synthase (gtlA), a central enzyme in the citric acid cycle linked to aerobic respiration. Primers for a 225 bp fragment suitable for qPCR were tested for specificity and assay performance verified on multiple soils. Clone libraries of the PCR-amplified gtlA gene exhibited high diversity and recovered most major groups identified in a previous 16S rRNA gene study. Comparisons among bacterial communities based on gtlA sequencing using UniFrac revealed differences among the experimental soils studied. Conditions for gtlA qPCR were optimized and calibration curves were highly linear (R2 > 0.99) over six orders of magnitude (4.56 10^5 to 4.56 10^11 copies), with high amplification efficiencies (>1.7). We examined the performance of the gtlA qPCR across a variety of soils and ecosystems, spanning forests, old fields and agricultural areas. We were able to amplify gtlA genes in all tested soils, and detected differences in gtlA abundance within and among environments. These results indicate that a fully developed gtlA-targeted qPCR approach may have potential to link microbial community characteristics with changes in soil respiration.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2015

Design and Construction of Large Size Micromegas Chambers for the ATLAS Upgrade of the Muon Spectrometer

Large area Micromegas detectors will be employed for the first time in high-energy physics experiments. A total surface of about 150 m{sup 2} of the forward regions of the Muon Spectrometer of the ATLAS detector at LHC will be equipped with 8-layer Micromegas modules. Each module extends over a surface from 2 to 3 m{sup 2} for a total active area of 1200 m{sup 2}. Together with the small strip Thin Gap Chambers they will compose the two New Small Wheels, which will replace the innermost stations of the ATLAS end-cap muon tracking system in the 2018/19 shutdown. In order to achieve a 15% transverse momentum resolution for 1 TeV muons, in addition to an excellent intrinsic resolution, the mechanical precision of each plane of the assembled module must be as good as 30 μm along the precision coordinate and 80 μm perpendicular to the chamber. In the prototyping towards the final configuration two similar quadruplets with dimensions 1.2 x 0.5 m{sup 2} have been built with the same structure as foreseen for the NSW upgrade. It represents the first example of a Micromegas quadruplet ever built, realized using the resistive-strip technology and decoupling the amplification mesh from the readout structure. All readout planes are segmented into strips with a pitch of 400 μm for a total of 4096 strips. In two of the four planes the strips are inclined by 1.5 deg. and provide a measurement of the second coordinate. The design and construction procedure of the Micromegas modules will be presented, as well as the design for the assembly of modules onto the New Small Wheel. Emphasis will be given on the methods developed to achieve the challenging mechanical precision. Measurements of deformation on chamber prototypes as a function of thermal gradients, gas over-pressure and internal stress (mesh tension and module fixation on supports) will be also shown in comparison to simulation. These tests were essential in the development of the final design in order to minimize the effects of deformations. During installation and operation all deformations and relative misalignments will be monitored through an optical alignment system and compensated in the tracking software. The optical alignment concept will also be outlined. 2 (authors)

43 PARTICLE ACCELERATORS↗
DOE OSTI2020

Structural Effects of Postulated Hydrogen Explosions in Process Piping and Vessels - 20274

At Savannah River Site (SRS), preparations to startup new liquid waste operations and recent advances in understanding thermolytic hydrogen generation have led to several recent studies on the structural effects of postulated hydrogen explosions. This paper reports on seven representative piping and vessel cases that are evaluated for deflagrations, detonations, and deflagration-to-detonation transition (DDT) assuming the initial mixture is stoichiometric hydrogen and air. The evaluations are based on the approach used by the Department of Defense for analyzing structures subjected to accidental explosions and include inelasticity, dynamic structural amplification, and material strain-rate effects. Evaluation criteria consider the potential for repeated explosions causing fatigue but also consider that some degree of permanent plastic deformation is acceptable as long as rupture is prevented. Specific conclusions depend on the assumed pre-ignition pressure. If the pre-ignition pressure is atmospheric and if the components are designed to a code from the American Society of Mechanical Engineers, the components evaluated are expected to plastically deform but not rupture under the explosion scenarios considered. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2014

Digitalized two parametric system for gamma/neutron spectrometry - Paper 59

Many types of detectors like stilbene, NE-213 etc. in conjunction with photomultiplier loaded with low working resistance produce pulses of approximately 100 ns length and contain information about deposited particle in the trailing edge. Using fast analog to digital converters (ADC) and field-programmable gate array (FPGA) it is possible to create a spectrometric system working in mixed gamma and neutron fields which is not loaded dead time. The count rate of processed pulses can reach more than one million per second. Such a high count rate of processed pulses can be achieved due to the pulse processing is implemented in FPGA. The output of this pulse processing is amplitude which describes the energy of deposited particle and discrimination parameter whereby it is possible to discriminate photons and neutrons. To increase the dynamic range of energy of detectable particle the signal from photomultiplier is separated into two branches with different amplification. Each branch is digitalized by separate ADC. Components from which the system is composed are so light that the spectrometer can be easily transported. Its weight is less than 3 kilograms. Spectrometer was tested in the research reactor LR-0 in Rez near Prague (Czech Republic). The measured data was processed using deconvolution into a neutron flux density and compared with nowadays used analog spectrometer and simulation result. Measured neutron spectrum of Cf-252 is included. (authors)

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2016

Projected intensification of subseasonal temperature variability and heat waves in the Great Plains

In this paper, compared to changes in the climatological mean temperature, we have less confidence in how much and by what mechanisms temperature variability may be affected by anthropogenic climate change. Here based on a 30-member climate change projection from an earth system model, we find that summertime subseasonal temperature variability in the U.S. Great Plains is enhanced by approximately 20% in 2070–2100 relative to 1980–2010. In particular, daily temperature departures from the new climatologies during future heat waves are on average 0.6°C warmer than are the corresponding departures under present-day conditions. Although in both periods heat waves in the Great Plains tend to be associated with planetary wave events, the amplification of future heat waves does not appear to be induced by changes in planetary wave variability in the midlatitudes. Instead, in this experiment the strengthening appears to be primarily caused by enhanced local land-atmosphere feedbacks resulting from a warmer/drier future climate.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2017

Interactions between land use change and carbon cycle feedbacks: Land Use and Carbon Cycle Feedbacks

We explore the role of human land use and land cover change (LULCC) in modifying the terrestrial carbon budget in simulations forced by Representative Concentration Pathway 8.5, extended to year 2300 by using the Community Earth System Model, . Overall, conversion of land (e.g., from forest to croplands via deforestation) results in a model-estimated, cumulative carbon loss of 490 Pg C between 1850 and 2300, larger than the 230 Pg C loss of carbon caused by climate change over this same interval. The LULCC carbon loss is a combination of a direct loss at the time of conversion and an indirect loss from the reduction of potential terrestrial carbon sinks. Approximately 40% of the carbon loss associated with LULCC in the simulations arises from direct human modification of the land surface; the remaining 60% is an indirect consequence of the loss of potential natural carbon sinks. Because of the multicentury carbon cycle legacy of current land use decisions, a globally averaged amplification factor of 2.6 must be applied to 2015 land use carbon losses to adjust for indirect effects. This estimate is 30% higher when considering the carbon cycle evolution after 2100. Most of the terrestrial uptake of anthropogenic carbon in the model occurs from the influence of rising atmospheric CO 2 on photosynthesis in trees, and thus, model-projected carbon feedbacks are especially sensitive to deforestation.

58 GEOSCIENCES↗
DOE OSTI2016

Van Allen Probes observations of electromagnetic ion cyclotron waves triggered by enhanced solar wind dynamic pressure

Magnetospheric compression due to impact of enhanced solar wind dynamic pressure P dyn has long been considered as one of the generation mechanisms of electromagnetic ion cyclotron (EMIC) waves. Here, with the Van Allen Probe-A observations, we identify three EMIC wave events that are triggered by P dyn enhancements under prolonged northward interplanetary magnetic field (IMF) quiet time preconditions. They are in contrast to one another in a few aspects. Event 1 occurs in the middle of continuously increasing P dyn while Van Allen Probe-A is located outside the plasmapause at postmidnight and near the equator (magnetic latitude (MLAT) ~ -3°). Event 2 occurs by a sharp P dyn pulse impact while Van Allen Probe-A is located inside the plasmapause in the dawn sector and rather away from the equator (MLAT ~ 12°). Event 3 is characterized by amplification of a preexisting EMIC wave by a sharp P dyn pulse impact while Van Allen Probe-A is located outside the plasmapause at noon and rather away from the equator (MLAT ~ -15°). These three events represent various situations where EMIC waves can be triggered by P dyn increases. Several common features are also found among the three events. (i) The strongest wave is found just above the He + gyrofrequency. (ii) The waves are nearly linearly polarized with a rather oblique propagation direction (~28° to ~39° on average). (iii) The proton fluxes increase in immediate response to the P dyn impact, most significantly in tens of keV energy, corresponding to the proton resonant energy. (iv) The temperature anisotropy with T ⊥ > T || is seen in the resonant energy for all the events, although its increase by the P dyn impact is not necessarily always significant. Finally, the last two points (iii) and (iv) may imply that in addition to the temperature anisotropy, the increase of the resonant protons must have played a critical role in triggering the EMIC waves by the enhanced P dyn impact.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

Effects of explicit convection on global land-atmosphere coupling in the superparameterized CAM

Here, conventional global climate models are prone to producing unrealistic land-atmosphere coupling signals. Cumulus and convection parameterizations are natural culprits but the effect of bypassing them with explicitly resolved convection on global land-atmosphere coupling dynamics has not been explored systematically. We apply a suite of modern land-atmosphere coupling diagnostics to isolate the effect of cloud Superparameterization in the Community Atmosphere Model (SPCAM) v3.5, focusing on both the terrestrial segment (i.e., soil moisture and surface turbulent fluxes interaction) and atmospheric segment (i.e., surface turbulent fluxes and precipitation interaction) in the water pathway of the landatmosphere feedback loop. At daily timescales, SPCAM produces stronger uncoupled terrestrial signals (negative sign) over tropical rainforests in wet seasons, reduces the terrestrial coupling strength in the Central Great Plain in American, and reverses the coupling sign (from negative to positive) over India in the boreal summer season—all favorable improvements relative to reanalysis-forced land modeling. Analysis of the triggering feedback strength (TFS) and amplification feedback strength (AFS) shows that SPCAM favorably reproduces the observed geographic patterns of these indices over North America, with the probability of afternoon precipitation enhanced by high evaporative fraction along the eastern United States and Mexico, while conventional CAM does not capture this signal. We introduce a new diagnostic called the Planetary Boundary Layer (PBL) Feedback Strength (PFS), which reveals that SPCAM exhibits a tight connection between the responses of the lifting condensation level, the PBL height, and the rainfall triggering to surface turbulent fluxes; a triggering disconnect is found in CAM.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2017

The role of the Southern Hemisphere semiannual oscillation in the development of a precursor to central and eastern Pacific Southern Oscillation warm events

The semiannual oscillation (SAO) is a twice-yearly northward movement (in May-June-July (MJJ) and November-December-January (NDJ)) of the circumpolar trough of sea level pressure (SLP) in the Southern Hemisphere with effects throughout the troposphere. During MJJ the second harmonic of SLP, describing the SAO, has low values of SLP north of 50°S in the subtropical South Pacific, while the first harmonic, which is dominant over the Australian sector, increases to its peak. This once-a-year peak in negative SLP gradients (decreasing to the east) between Australia and the ocean to its east extends to the equatorial Pacific. Southern Oscillation warm events since 1950, with an intensification of this seasonal cycle, have had larger-amplitude SST anomalies in the eastern equatorial Pacific in MJJ and during the following mature phase in NDJ. Weak amplification of the seasonal cycle in MJJ tends to be followed by larger-amplitude SST anomalies in the central equatorial Pacific during NDJ.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2017

Systematic Evaluation of Low-Frequency Hiss and Energetic Electron Injections

Here, the excitation of low-frequency (LF) plasmaspheric hiss, over the frequency range from 20 Hz to 100 Hz, is systematically investigated by comparing the hiss wave properties with electron injections at energies from tens of keV to several hundreds of keV. Both particle and wave data from the Van Allen Probes during the period from September 2012 to June 2016 are used in the present study. Our results demonstrate that the intensity of LF hiss has a clear day-night asymmetry, and increases with increasing geomagnetic activity, similar to the behavior of normal hiss (approximately hundred of hertz to several kilohertz). The occurrence rate of LF hiss in association with electron injections is up to 80% in the outer plasmasphere ( L > 4) on the dayside, and the strong correlation extends to lower L shells for more active times. In contrast, at lower L shells ( L < 3.5), LF hiss is seldom associated with electron injections. The LF hiss with Poynting flux directed away from the equator is dominant at higher magnetic latitudes and higher L shells, suggesting a local amplification of LF hiss in the outer plasmasphere. The averaged electron fluxes are larger at higher L shells, where significant LF hiss wave events are observed. Our study suggests the importance of electron injections and their drift trajectories toward the dayside plasmasphere in locally amplifying the LF hiss waves detected by the Van Allen Probes.

58 GEOSCIENCES↗
DOE OSTI2017

Dynamics of simulated Atlantic upwelling annual cycle in CMIP5 models

As one of the prominent climatic component of tropical climate system, the annual cycle of the equatorial Atlantic cold tongue region has been a subject of significant research. In this work, we examine the fidelity of the key part of the annual cycles, namely, the upwelling annual cycles, from the simulations of the Coupled Model Intercomparison Project Phase 5 (CMIP5). Utilizing the simple framework we have recently developed based on the Zebiak–Cane (ZC) ocean model, we first tested the validity of the simple framework of upwelling as a combination of the local wind–driven Ekman upwelling and nonlocal wind–driven wave upwelling. It is demonstrated that the theoretically reconstructed upwelling annual cycles to large extent are in good agreement with the simulations in these coupled climatic models. Comparing with observations, we show that the semiannual component of the upwelling is systematically too strong. This significant bias is attributable to the dynamic amplification to the wind–driven wave upwelling originated from a less significant bias in the semiannual harmonic component in the central equatorial wind field. The latter is dynamically amplified in the wind–driven wave upwelling owing to its relatively fast time scale. Furthermore, the realistic simulations of equatorial annual cycles may sensitively depend on good simulations of the semiannual harmonic components in equatorial wind fields.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2017

Daily-scale planetary wave patterns and the modulation of cold season weather in the northern extratropics

Here, tropospheric planetary waves, often linked to asymmetries in lower boundary forcing,significantly modulate atmospheric blocking and storm track structures that are, in turn, linked to extremesurface weather events. Day-to-day variability in the planetary scale circulation, taken as wave numbers 1 to 5in the daily 500 hPa geopotential heights, and the associated impact on storm tracks and regional weather arestudied for the period 1950–2005. Six boreal cold-season distinct planetary wave patterns are identified viahierarchical cluster analysis. The first, second, and sixth patterns feature a prominent zonal wave number 1structure, while the fourth and fifth patterns resemble the negative and positive phases of northern annularmode, respectively. The second pattern represents an amplification of the climatological mean wavestructure, while the third pattern resembles the zonal wave number 3 pattern. A multitaper spectral analysisof the daily projection indices indicates that the planetary wave patterns are primarily intraseasonal innature. The first (sixth) pattern combines the positive (negative) phase of the Pacific-North Americanteleconnection pattern and negative (positive) phase of the North Atlantic Oscillation, inducing poleward(equatorward) shifts in the Pacific storm track and a weakened (strengthened) Atlantic storm track. Incontrast, the fourth (fifth) pattern results in a simultaneous equatorward (poleward) displacement of bothstorm tracks. Extreme cold waves over the continental United States (U.S.) are favored during occurrences ofthe fourth and sixth patterns. During episodes of the first pattern, increased rainfall prevails over much ofthe U.S., while precipitation anomalies induced by the other patterns are more regional in nature.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2017

Emergent Behavior of Arctic Precipitation in Response to Enhanced Arctic Warming: Enhanced Arctic Precipitation

Amplified warming of the high latitudes in response to human-induced emissions of greenhouse gases has already been observed in the historical record and is a robust feature evident across a hierarchy of model systems, including the models of the Coupled Model Intercomparison Project Phase 5 (CMIP5). The main aims of this analysis are to quantify intermodel differences in the Arctic amplification (AA) of the global warming signal in CMIP5 RCP8.5 (Representative Concentration Pathway 8.5) simulations and to diagnose these differences in the context of the energy and water cycles of the region. This diagnosis reveals an emergent behavior between the energetic and hydrometeorological responses of the Arctic to warming: in particular, enhanced AA and its associated reduction in dry static energy convergence is balanced to first order by latent heating via enhanced precipitation. This balance necessitates increasing Arctic precipitation with increasing AA while at the same time constraining the magnitude of that precipitation increase. The sensitivity of the increase, ~1.25 (W/m 2 )/K (~240 (km 3 /yr)/K), is evident across a broad range of historical and projected AA values. Accounting for the energetic constraint on Arctic precipitation, as a function of AA, in turn informs understanding of both the sign and magnitude of hydrologic cycle changes that the Arctic may experience.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

Multimodel Surface Temperature Responses to Removal of U.S. Sulfur Dioxide Emissions

Abstract Three Earth System models are used to derive surface temperature responses to removal of U.S. anthropogenic SO 2 emissions. Using multicentury perturbation runs with and without U.S. anthropogenic SO 2 emissions, the local and remote surface temperature changes are estimated. In spite of a temperature drift in the control and large internal variability, 200 year simulations yield statistically significant regional surface temperature responses to the removal of U.S. SO 2 emissions. Both local and remote surface temperature changes occur in all models, and the patterns of changes are similar between models for northern hemisphere land regions. We find a global average temperature sensitivity to U.S. SO 2 emissions of 0.0055 K per Tg(SO 2 ) per year with a range of (0.0036, 0.0078). We examine global and regional responses in SO 4 burdens, aerosol optical depths (AODs), and effective radiative forcing (ERF). While changes in AOD and ERF are concentrated near the source region (United States), the temperature response is spread over the northern hemisphere with amplification of the temperature increase toward the Arctic. In all models, we find a significant response of dust concentrations, which affects the AOD but has no obvious effect on surface temperature. Temperature sensitivity to the ERF of U.S. SO 2 emissions is found to differ from the models' sensitivity to radiative forcing of doubled CO 2 .

Conley, A. J.↗
DOE OSTI2015

Lasers, extreme UV and soft X-ray

Three decades ago, large ICF lasers that occupied entire buildings were used as the energy sources to drive the first X-ray lasers. Today X-ray lasers are tabletop, spatially coherent, high-repetition rate lasers that enable many of the standard optical techniques such as interferometry to be extended to the soft X-ray regime between wavelengths of 10 and 50 nm. Over the last decade X-ray laser performance has been improved by the use of the grazing incidence geometry, diode-pumped solid-state lasers, and seeding techniques. The dominant X-ray laser schemes are the monopole collisional excitation lasers either driven by chirped pulse amplification (CPA) laser systems or capillary discharge. The CPA systems drive lasing in neon-like or nickel-like ions, typically in the 10 – 30 nm range, while the capillary system works best for neon-like argon at 46.9 nm. Most researchers use nickel-like ion lasers near 14 nm because they are well matched to the Mo:Si multilayer mirrors that have peak reflectivity near 13 nm and are used in many applications. As a result, the last decade has seen the birth of the X-ray free electron laser (XFEL) that can reach wavelengths down to 0.15 nm and the inner-shell Ne laser at 1.46 nm.

42 ENGINEERING↗
DOE OSTI2021

Universal Approach to Rapid Amplified Plasmonic Sensing Using Helix Defect Phase Transition Polymers

Abstract Permittivity sensing is a critically important analytical tool for bioscience, environmental, and industrial applications. The response time for commonly used plasmonic permittivity sensors is fundamentally set by the reaction kinetics of chemically adsorbed analytes. In this work, the proposal is to overcome this limit by combining plasmonic sensors with phase transition materials possessing a rapid amplified electrostatic response such as quadrupole moment induced molecular helix reversal. As a proof‐of‐concept, rapid sensing of CO 2 on a phase transition polytetrafluoroethylene substrate and amplification of permittivity response in plasmonic Fabry–Perot sensor is shown. The demonstrated universal approach holds promise for a wide range of applications in fast, real time sensing and monitoring of biological and environmental processes.

Shugayev, Roman↗