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All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

Analysis of the NASA White Sands Test Facility (WSTF) Test System for Friction-Ignition of Metallic Materials

Friction is a known ignition source for metals in oxygen-enriched atmospheres. The test system developed by the NASA White Sands Test Facility in response to ASTM G-94 has been used successfully to determine the relative ignition from friction of numerous metallic materials and metallic materials pairs. These results have been ranked in terms of a pressure-velocity product (PV) as measured under the prescribed test conditions. A high value of 4.1(exp 8) watts per square meter for Inconel MA 754 is used to imply resistance to friction ignition, whereas a low value of 1.04(exp 8) watts per square meter for stainless steel 304 is taken as indicating material susceptible to friction ignition. No attempt has been made to relate PV values to other material properties. This work reports the analysis of the WSTF friction-ignition test system for producing fundamental properties of metallic materials relating to ignition through friction. Three materials, aluminum, titanium, and nickel were tested in the WSTF frictional ignition instrument system under atmospheres of oxygen or nitrogen. Test conditions were modified to reach a steady state of operation, that is applied, the force was reduced and the rotational speed was reduced. Additional temperature measurements were made on the stator sample. The aluminum immediately galled on contact (reproducible) and the test was stopped. Titanium immediately ignited as a result of non-uniform contact of the stator and rotor. This was reproducible. A portion of the stator sampled burned, but the test continued. Temperature measurements on the stator were used to validate the mathematical model used for estimating the interface (stator/rotor) temperature. These interface temperature measurements and the associate thermal flux into the stator were used to distinguish material-phase transitions, chemical reaction, and mechanical work. The mechanical work was used to analyze surface asperities in the materials and to estimate a coefficient of fiction. The coefficient of fiction was analyzed in terms of material properties that is, hardness, Young's modulus and elasticity/plasticity of the material.

Shoffstall, Michael S.↗
NASA NTRS2001

Evolution and Activity in the Solar Corona: A Comparison of Coronal and Chromospheric Structures Seen in Soft X-Rays, White Light and H-Alpha Emission

The work completed under this project, 'Evolution and Activity in the Solar Corona: A Comparison of Coronal and Chromospheric Structures Seen in Soft X-Rays, White Light and H-Alpha Emission', includes the following presentations: (1) Analysis of H-alpha Observations of High-altitude Coronal Condensations; (2) Multi-spectral Imaging of Coronal Activity; (3) Measurement and Modeling of Soft X-ray Loop Arcades; (4) A Study of the Origin and Dynamics of CMEs; and various poster presentations and thesis dissertations.

Bagenal, Fran↗
NASA NTRS2001

Preparation and Preliminary Characterization of Crystallizing Fluorescent Derivatives of Chicken Egg White Lysozyme

Fluorescence is one of the most versatile and powerful tools for the study of macromolecules. While most proteins are intrinsically fluorescent, working at crystallization concentrations require the use of covalently prepared derivatives added as tracers. This approach requires derivatives that do not markedly affect the crystal packing. We have prepared fluorescent derivatives of chicken egg white lysozyme with probes bound to one of two different sites on the protein molecule. Lucifer yellow and 5-(2-aminoethyl)aminonapthalene-1-sulfonic acid (EDANS) have been attached to the side chain carboxyl of Asp(sup 101) using a carbodiimide coupling procedure. Asp(sup 101) lies within the active site cleft, and it is believed that the probes are "buried" within that cleft. Lucifer yellow and MANS probes with iodoacetamide reactive groups have been bound to His(sup 15), located on the "back side" of the molecule relative to the active site. All the derivatives fluoresce in the solution and the crystalline states. Fluorescence characterization has focused on determination of binding effects on the probe quantum yield, lifetime, absorption and emission spectra, and quenching by added solutes. Quenching studies show that, as postulated, the Asp(sup 101)-bound probes are partially sheltered from the bulk solution by their location within the active site cleft. Probes bound to His(sup 15) have quenching constants about equal to those for the free probes, indicating that this site is highly exposed to the bulk solution.

Sumida, John↗
NASA NTRS2001

The Effect of Solution Thermal History on Chicken Egg White Lysozyme Nucleation

Proteins are highly flexible molecules and often exhibit defined conformational changes in response to changes in the ambient temperature. Chicken egg white lysozyme has been previously shown to undergo an apparent structural change when warmed above the tetragonal/orthorhombic phase transition temperature. This is reflected by a change in the habit of the tetragonal and orthorhombic crystals so formed. In this study, we show that possible conformational changes induced by heating are stable and apparently non-reversible by simple cooling. Exposure of protein solutions to temperatures above the phase change transition temperature, before combining with precipitant solution to begin crystallization, reduces final crystal numbers. Protein that is briefly warmed to 37 C, then cooled shows no sign of reversal to the unheated nucleation behavior even after storage for four weeks at 4 C. The change in nucleation behavior of tetragonal lysozyme crystals, attributed to a structural shift, occurs faster the greater the exposure to temperature above the equi-solubility point for the two phases. Heating for 2 hours at 48 C reduces crystal numbers by 20 fold in comparison to the same solution heated for the same time at 30 C. Thermal treatment of solutions is therefore a possible tool to reduce crystal numbers and increase crystal size. The effects of a protein's previous thermal history are now shown to be a potentially critical factor in subsequent macromolecule crystal nucleation and growth studies.

Burke, Michael W.↗
NASA NTRS2001

Preparation and Preliminary Characterization of Crystallizing Fluorescent Derivatives of Chicken Egg White Lysozyme

Fluorescence is one of the most versatile and powerful tools for the study of macromolecules. While most proteins are intrinsically fluorescent, working at crystallization concentrations require the use of covalently prepared derivatives added as tracers. This approach requires derivatives that do not markedly affect the crystal packing. We have prepared fluorescent derivatives of chicken egg white lysozyme with probes bound to one of two different sites on the protein molecule. Lucifer yellow and 5-(2-aminoethyl)aminonapthalene-i-sulfonic acid (EDANS) have been attached to the side chain carboxyl of Asp(sup 101) using a carbodiimide coupling procedure. Asp(sup 101) lies within the active site cleft, and it is believed that the probes are 'buried' within that cleft. Lucifer yellow and MANS probes with iodoacetamide reactive five groups have been bound to His(sup 15), located on the 'back side' of the molecule relative to the active site. All the derivatives fluoresce in the solution and the crystalline states. Fluorescence characterization has focused on determination of binding effects on the probe quantum yield, lifetime, absorption and emission spectra, and quenching by added solutes. Quenching studies show that, as postulated, the Asp(sup 101)-bound probes are partially sheltered from the bulk solution by their location within the active site cleft. Probes bound to His(sup 15) have quenching constants about equal to those for the free probes, indicating that this site is highly exposed to the bulk solution.

Sumida, John P.↗
NASA NTRS1999

Buffer Effects in the Solubility, Nucleation and Growth of Chicken Egg White Lysozyme

The growth of protein crystals is important for determination of their three-dimensional structure, which relates to their biochemical functions and to the practical goal of designing pharmaceuticals to modify that function. While many proteins have been successfully crystallized by a variety of methods, there is still limited understanding of the process of nucleation and growth of even the simplest proteins. Chicken egg-white lysozyme (CEWL) is readily crystallized under a variety of conditions, and studies underway at MSFC are designed to elucidate the mechanisms by which the crystals nucleate and grow. We have investigated the effect of buffer choice on the solubility, nucleation and growth of CEWL. CEWL was purified by dialysis against a .05M phosphate buffer and chromatographic separation from contaminants in a sepharose column. Solubility studies were made as a function of buffer concentration for phosphate and formate buffers, and the nucleation and growth of crystals at 10 C was studied as a function of pH for oxalate, succinate, formate, butyrate, carbonate, phosphate and acetate buffer solutions. The solubility data support the conclusion that there is a solubility minimum as a function of buffer concentration for amphiphilic molecules, while no minimum is observed for a phosphate buffer. Nucleation is suppressed at pH greater than pKa for all buffers except phosphate. The aspect ratio of the (110) faces is shown to be a function of crystal size, rather than pH.

Gibson, Ursula J.↗
NASA NTRS2001

Mapping Arid Vegetation Species Distributions in the White Mountains, Eastern California, Using AVIRIS, Topography, and Geology

Our challenge is to model plant species distributions in complex montane environments using disparate sources of data, including topography, geology, and hyperspectral data. From an ecologist's point of view, species distributions are determined by local environment and disturbance history, while spectral data are 'ancillary.' However, a remote sensor's perspective says that spectral data provide picture of what vegetation is there, topographic and geologic data are ancillary. In order to bridge the gap, all available data should be used to get the best possible prediction of species distributions using complex multivariate techniques implemented on a GIS. Vegetation reflects local climatic and nutrient conditions, both of which can be modeled, allowing predictive mapping of vegetation distributions. Geologic substrate strongly affects chemical, thermal, and physical properties of soils, while climatic conditions are determined by local topography. As elevation increases, precipitation increases and temperature decreases. Aspect, slope, and surrounding topography determine potential insolation, so that south-facing slopes are warmer and north-facing slopes cooler at a given elevation. Topographic position (ridge, slope, canyon, or meadow) and slope angle affect sediment accumulation and soil depth. These factors combine as complex environmental gradients, and underlie many features of plant distributions. Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) data, digital elevation models, digitized geologic maps, and 378 ground control points were used to predictively map species distributions in the central and southern White Mountains, along the western boundary of the Basin and Range province. Minimum Noise Fraction (MNF) bands were calculated from the visible and near-infrared AVIRIS bands, and combined with digitized geologic maps and topographic variables using Canonical Correspondence Analysis (CCA). CCA allows for modeling species 'envelopes' in multidimensional environmental space, which can then be projected across entire landscapes.

VandeVen, C.↗
NASA NTRS2002

Heat Flow, Thermal Conductivity, and the Plausibility of the White Mars Hypothesis

Due to the low thermal conductivity of CO2 ice and clathrate vs. water ice, we find that liquid water reservoirs would not be confined to the deep subsurface as predicted by the controversial White Mars model, even assuming low global heat flow. Additional information is contained in the original extended abstract.

Urquhart, M. L.↗
NASA NTRS2002

Hypersonic Airbreathing Propulsion: An Aerodynamics, Aerothermodynamics, and Acoustics Competency White Paper

This White Paper examines the current state of Hypersonic Airbreathing Propulsion at the NASA Langley Research Center and the factors influencing this area of work and its personnel. Using this knowledge, the paper explores beyond the present day and suggests future directions and strategies for the field. Broad views are first taken regarding potential missions and applications of hypersonic propulsion. Then, candidate propulsion systems that may be applicable to these missions are suggested and discussed. Design tools and experimental techniques for developing these propulsion systems are then described, and approaches for applying them in the design process are considered. In each case, current strategies are reviewed and future approaches that may improve the techniques are considered. Finally, the paper concentrates on the needs to be addressed in each of these areas to take advantage of the opportunities that lay ahead for both the NASA Langley Research Center and the Aerodynamic Aerothermodynamic, and Aeroacoustics Competency. Recommendations are then provided so that the goals set forth in the paper may be achieved.

Drummond, J. Philip↗
NASA NTRS2002

Experimental Evaluation of White Light Fabry-Perot Interferometry Fiber-Optic Strain Gages when Measuring Small Strains

An experimental study was conducted to evaluate whether fiber optic strain gages (FOSG) are "better" sensors than typical foil gages. A particularly attractive feature of FOSG was their specified resolution of 0.01% of full-scale (0.1 micro strain for 1000 micro strain full-scale). This feature would make FOSG practical tank level sensors, by measuring very small strains on the support structure of a tank. A specific application in mind was to measure liquid oxygen tank level, with support beams that were predicted to contract approximately 11 micro strain as the tank goes from empty to full. Among various fiber optic technologies currently available, Fabry-Perot Interferometry using white light was selected. This technology exhibits highly desirable feature such as absolute strain measurement, linearity over its full-scale, and temperature compensation. However, experiment results suggest that the resolution is 0.8 micro strain, at best, calibration from one sensor to another can be off by 2.4 - 11.2%, and that temperature compensation is not fully predictable, with errors of up to 3.5 micro strain over an 11C range. Hence, when compared with classic foil gages, FOSG possess less accuracy, similar resolution and repeatability (precision), and superior linearity over their entire operating range. They are immune to EMI and their signals suffer minimal degradation over long distances. It is also expected that drift with time will be minimal in FOSG whereas the gage factor of foil sensors changes over time when exposed to varying environmental conditions. In conclusion, FOSG are "better" than foil gages as long as the application allows calibration of individual units as installed for operation.

St.Cyr, William↗
NASA NTRS2004

CHANDRA Observations of RX J1914.4+ 2456: Spin-up of a White Dwarf?

RX 51914.4+2456 is a candidate double-degenerate binary with a putative 1.756 mHz orbital frequency. In a previous timing study using archival ROSAT and ASCA data we reported evidence for an increase of the putative orbital frequency at a rate consistent with expectations for gravitational radiation from the system. Here we report the results of new Chandra timing observations which confirm the previous indications of spin-up of the X-ray frequency, and provide much tighter constraints on the frequency derivative, u. We obtained with Chandra a total of 75 ksec of exposure in two epochs separated in time by 10.3 months. The total time span of the archival ROSAT, ASCA and new Chandra data is now 10.2 years. This more than doubles the interval spanned by the ROSAT and ASCA data alone, providing much greater sensitivity to a frequency derivative. With the addition of the Chandra data an increasing frequency is unavoidable, and the mean i/ is 5.9f0.9 x 10-l' Hz s-'. Interestingly, power spectra of the longest Chandra pointing show evidence for a sideband structure to the 1.756 mHz frequency. The fundamental and first harmonic show evidence for upper sidebands with a frequency separation of E 0.5 mHz from their parent peaks. Additionally, the first and second harmonics show evidence for lower sidebands with approximately half the frequency separation of the upper sidebands. Similar sideband structure is a common feature of Intermediate Polars (Ips)-although it is usually observed in the optical-and suggests the presence of a longer period in the system, perhaps the previously unseen orbital period. If this is correct the sideband structure indicates an orbital period close to 1 hr, and the observed u likely represents the accretion-induced spin-up of a white dwarf. We discuss the implications of these findings for the nature of RX J1914.4+2456.

Strohmayer, Tod E.↗
NASA NTRS2003

Standards Development Activities at White Sands Test Facility

The development of standards and standard activities at the JSC White Sands Test Facility (WSTF) has been expanded to include the transfer of technology and standards to voluntary consensus organizations in five technical areas of importance to NASA. This effort is in direct response to the National Technology Transfer Act designed to accelerate transfer of technology to industry and promote government-industry partnerships. Technology transfer is especially important for WSTF, whose longterm mission has been to develop and provide vital propellant safety and hazards information to aerospace designers, operations personnel, and safety personnel. Meeting this mission is being accomplished through the preparation of consensus guidelines and standards, propellant hazards analysis protocols, and safety courses for the propellant use of hydrogen, oxygen, and hypergols, as well as the design and inspection of spacecraft pressure vessels and the use of pyrovalves in spacecraft propulsion systems. The overall WSTF technology transfer program is described and the current status of technology transfer activities are summarized.

Baker, D. L.↗
NASA NTRS1999

Early root cap development and graviresponse in white clover (Trifolium repens) grown in space and on a two-axis clinostat

White clover (Trifolium repens) was germinated and grown in microgravity aboard the Space Shuttle (STS-60, 1994; STS-63, 1995), on Earth in stationary racks and in a slow-rotating two-axis clinostat. The objective of this study was to determine if normal root cap development and early plant gravity responses were dependent on gravitational cues. Seedlings were germinated in space and chemically fixed in orbit after 21, 40, and 72 h. Seedlings 96 h old were returned viable to earth. Germination and total seedling length were not dependent on gravity treatment. In space-flown seedlings, the number of cell stories in the root cap and the geometry of central columella cells did not differ from those of the Earth-grown seedlings. The root cap structure of clinorotated plants appeared similar to that of seedlings from microgravity, with the exception of three-day rotated plants, which displayed significant cellular damage in the columella region. Nuclear polarity did not depend on gravity; however, the positions of amyloplasts in the central columella cells were dependent on both the gravity treatment and the age of the seedlings. Seedlings from space, returned viable to earth, responded to horizontal stimulation as did 1 g controls, but seedlings rotated on the clinostat for the same duration had a reduced curvature response. This study demonstrates that initial root cap development is insensitive to either chronic clinorotation or microgravity. Soon after differentiation, however, clinorotation leads to loss of normal root cap structure and plant graviresponse while microgravity does not.

manned↗
NASA NTRS1997

Modulation of statolith mass and grouping in white clover (Trifolium repens) growth in 1-g, microgravity and on the clinostat

Current models of gravity perception in higher plants focus on the buoyant weight of starch-filled amyloplasts as the initial gravity signal susceptor (statolith). However, no tests have yet determined if statolith mass is regulated to increase or decrease gravity stimulus to the plant. To this end, the root caps of white clover (Trifolium repens) grown in three gravity environments with three different levels of gravity stimulation have been examined: (i) 1-g control with normal static gravistimulation, (ii) on a slow clinostat with constant gravistimulation, and (iii) in the stimulus-free microgravity aboard the Space Shuttle. Seedlings were germinated and grown in the BioServe Fluid Processing Apparatus and root cap structure was examined at both light and electron microscopic levels, including three-dimensional cell reconstruction from serial sections. Quantitative analysis of the electron micrographs demonstrated that the starch content of amyloplasts varied with seedling age but not gravity condition. It was also discovered that, unlike in starch storage amyloplasts, all of the starch granules of statolith amyloplasts were encompassed by a fine filamentous, ribosome-excluding matrix. From light micrographic 3-D cell reconstructions, the absolute volume, number, and positional relationships between amyloplasts showed (i) that individual amyloplast volume increased in microgravity but remained constant in seedlings grown for up to three days on the clinostat, (ii) the number of amyloplasts per cell remained unchanged in microgravity but decreased on the clinostat, and (iii) the three-dimensional positions of amyloplasts were not random. Instead amyloplasts in microgravity were grouped near the cell centers while those from the clinostat appeared more dispersed. Taken together, these observations suggest that changing gravity stimulation can elicit feedback control over statolith mass by changing the size, number, and grouping of amyloplasts. These results support the starch-statolith theory of graviperception in higher plants and add to current models with a new feedback control loop as a mechanism for modulation of statolith responsiveness to inertial acceleration.

Flight Experiment↗
NASA NTRS1997

Visual signal detection in structured backgrounds. II. Effects of contrast gain control, background variations, and white noise

Studies of visual detection of a signal superimposed on one of two identical backgrounds show performance degradation when the background has high contrast and is similar in spatial frequency and/or orientation to the signal. To account for this finding, models include a contrast gain control mechanism that pools activity across spatial frequency, orientation and space to inhibit (divisively) the response of the receptor sensitive to the signal. In tasks in which the observer has to detect a known signal added to one of M different backgrounds grounds due to added visual noise, the main sources of degradation are the stochastic noise in the image and the suboptimal visual processing. We investigate how these two sources of degradation (contrast gain control and variations in the background) interact in a task in which the signal is embedded in one of M locations in a complex spatially varying background (structured background). We use backgrounds extracted from patient digital medical images. To isolate effects of the fixed deterministic background (the contrast gain control) from the effects of the background variations, we conduct detection experiments with three different background conditions: (1) uniform background, (2) a repeated sample of structured background, and (3) different samples of structured background. Results show that human visual detection degrades from the uniform background condition to the repeated background condition and degrades even further in the different backgrounds condition. These results suggest that both the contrast gain control mechanism and the background random variations degrade human performance in detection of a signal in a complex, spatially varying background. A filter model and added white noise are used to generate estimates of sampling efficiencies, an equivalent internal noise, an equivalent contrast-gain-control-induced noise, and an equivalent noise due to the variations in the structured background.

NASA Discipline Space Human Factors↗
NASA NTRS1997

Radiation-induced chromosomal instability in BALB/c and C57BL/6 mice: the difference is as clear as black and white

Genomic instability has been proposed to be the earliest step in radiation-induced tumorigenesis. It follows from this hypothesis that individuals highly susceptible to induction of tumors by radiation should exhibit enhanced radiation-induced instability. BALB/c white mice are considerably more sensitive to radiation-induced mammary cancer than C57BL/6 black mice. In this study, primary mammary epithelial cell cultures from these two strains were examined for the "delayed" appearance of chromosomal aberrations after exposure to 137Cs gamma radiation, as a measure of radiation-induced genomic instability. As expected, actively dividing cultures from both strains showed a rapid decline of initial asymmetrical aberrations with time postirradiation. However, after 16 population doublings, cells from BALB/c mice exhibited a marked increase in the frequency of chromatid-type breaks and gaps which remained elevated throughout the time course of the experiment (28 doublings). No such effect was observed for the cells of C57BL/6 mice; after the rapid clearance of initial aberrations, the frequency of chromatid-type aberrations in the irradiated population remained at or near those of nonirradiated controls. These results demonstrate a correlation between the latent expression of chromosomal damage in vitro and susceptibility for mammary tumors, and provide further support for the central role of radiation-induced instability in the process of tumorigenesis.

NASA Discipline Radiation Health↗
NASA NTRS1994

Conventional and cross-correlation brain-stem auditory evoked responses in the white leghorn chick: rate manipulations

Rate-dependent changes in the chick brain-stem auditory evoked response (BAER) using conventional averaging and a cross-correlation technique were investigated. Five 15- to 19-day-old white leghorn chicks were anesthetized with Chloropent. In each chick, the left ear was acoustically stimulated. Electrical pulses of 0.1-ms duration were shaped, attenuated, and passed through a current driver to an Etymotic ER-2 which was sealed in the ear canal. Electrical activity from stainless-steel electrodes was amplified, filtered (300-3000 Hz) and digitized at 20 kHz. Click levels included 70 and 90 dB peSPL. In each animal, conventional BAERs were obtained at rates ranging from 5 to 90 Hz. BAERs were also obtained using a cross-correlation technique involving pseudorandom pulse sequences called maximum length sequences (MLSs). The minimum time between pulses, called the minimum pulse interval (MPI), ranged from 0.5 to 6 ms. Two BAERs were obtained for each condition. Dependent variables included the latency and amplitude of the cochlear microphonic (CM), wave 2 and wave 3. BAERs were observed in all chicks, for all level by rate combinations for both conventional and MLS BAERs. There was no effect of click level or rate on the latency of the CM. The latency of waves 2 and 3 increased with decreasing click level and increasing rate. CM amplitude decreased with decreasing click level, but was not influenced by click rate for the 70 dB peSPL condition. For the 90 dB peSPL click, CM amplitude was uninfluenced by click rate for conventional averaging. For MLS BAERs, CM amplitude was similar to conventional averaging for longer MPIs.(ABSTRACT TRUNCATED AT 250 WORDS).

NASA Discipline Neuroscience↗
NASA NTRS2005

A Survey of Helium Accreting White Dwarfs

We have initiated a survey of the X-ray spectral properties of double white binaries using XMM-Newton. Three of our sources were indeed observed with XMM during AO-3. We have analyzed these data using the latest data reduction software and have also extracted several archival data sets of similar systems from the XMM archive. The first paper presenting the X-ray spectral and variability properties of four of these binary systems has been submitted in January 2005. We have also secured some optical ground-based spectroscopy and are currently analyzing the spectral signatures in more detail. We are also continuing this survey with additional approved XMM observations during AO-4 and anticipate additional publications in the near future.

Steeghs, Daniel↗