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At least 1,351 records · Page 75

Reliability-informed end-of-use decision making for product sustainability using two-stage stochastic optimization

The concept of circular economy has been diffused in recent decades to promote economic growth that does not add to the burden on natural resource extraction. Re-X options (e.g., reuse, repair, refurbish, remanufacture, recycle) have been gradually adopted in the product development process and optimized to reduce or eliminate waste and pollution. Although manufacturing incorporating Re-X options can be more environmentally friendly, it involves more sources of uncertainty than traditional manufacturing since the end-of-use products can be collected from multiple origins with various quantities and qualities, and the market demand for both new and remanufactured products cannot be forecasted perfectly. Thus, there is a need to optimize the Re-X policy to alleviate the negative impacts of the higher uncertainty. One option is using the reliability information of new products to estimate the end-of-use conditions and applying multi-stage stochastic optimization to capture multiple demand scenarios. This paper develops a two-stage stochastic optimization model to optimize the quality thresholds for reuse, recycling, and remanufacturing options. Our objective is to minimize the total cost, energy consumption, and environmental impact of producing and providing warranty service for a product family. The model employs reliability information of product components to estimate the warranty service cost and the end-of-use conditions of the returned resources. A case study on a general product family is implemented to illustrate the efficacy of the optimization model. Finally, results show that the two-stage optimization can achieve cost and environmental impact reduction for a hybrid manufacturing and remanufacturing process.

97 MATHEMATICS AND COMPUTING↗

NF-κB signaling and cell-fate decision induced by a fast-dissociating tumor necrosis factor mutant

Tumor necrosis factor (TNF) is a pluripotent inflammatory cytokine that can induce both the pro-survival nuclear factor kappa B (NF-κB) pathway and the pro-apoptotic caspase pathway. Selectively activating only one of the two pathways remains challenging. We used TNF mutants with different receptor binding kinetics to study their effects on NF-κB signaling dynamics and cell apoptosis. A TNF mutant, R1antTNF, which binds to TNFR1 with increased association and dissociation rates, induced NF-κB signaling with shorter response time and first peak duration. The short nuclear stay of NF-κB led to biased activation of downstream genes, favoring the fast response ones. At the same time, R1antTNF retains pro-apoptotic activity. At 10 ng/ml, R1antTNF selectively activated the pro-apoptotic pathway rather than the pro-survival NF-κB pathway. Our study provides a new example for the emerging evidence that ligand-receptor binding kinetics play a key role in the selective activation of downstream pathways, which deserves more attention in future drug discovery and disease studies. - Highlights: • Receptor-TNF and mutant binding kinetics changes lead to functional selectivity. • R1antTNF is a fast associating and fast dissociating TNF mutant. • R1antTNF produces shortened nuclear duration of NF-κB. • R1antTNF induces biased gene expression of early-response genes. • R1antTNF selectively activates apoptosis at low concentration.

60 APPLIED LIFE SCIENCES↗

The impact of ancillary services in optimal DER investment decisions

Microgrid resource sizing problems typically include the analysis of a combination of value streams such as peak shaving, load shifting, or load scheduling, which support the economic feasibility of the microgrid deployment. However, microgrid benefits can go beyond these, and the ability to provide ancillary grid services such as frequency regulation or spinning and non-spinning reserves is well known, despite typically not being considered in resource sizing problems. This paper proposes the expansion of the Distributed Energy Resources Customer Adoption Model (DER-CAM), a state-of-the-art microgrid resource sizing model, to include revenue streams resulting from the participation in ancillary service markets. Results suggest that participation in such markets may not only influence the optimum resource sizing, but also the operational dispatch, with results being strongly influenced by the exact market requirements and clearing prices.

24 POWER TRANSMISSION AND DISTRIBUTION↗