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At least 1,351 records · Page 75
All sourcesNASA NTRSDOE OSTI
NASA NTRS2018

Pilot Study on the Investigation of Tear Fluid Biomarkers as an Indicator of Ocular, Neurological, and Immunological Health in Astronauts

The purpose of this pilot study is to investigate the collection, preparation, and analysis of tear biomarkers as a means of assessing ocular, neurological, and immunological health. At present, no published data exists on the cytokine profiles of tears from astronauts exposed to long periods of microgravity and space irradiations. In addition, no published data exist on cytokine (biomarker) profiles of tears that have been collected from irradiated non-human biological systems (primates and other animal models). A goal for the proposed pilot study is to discover novel tear biomarkers which can help inform researchers, clinicians, epidemiologist and healthcare providers about the health status of a living biological system, as well as informing them when a disease state is triggered. This would be done via analysis of the onset of expression of pro-inflammatory cytokines, leading up to the full progression of a disease (i.e. cancer, loss of vision, radiation-induced oxidative stress, cardiovascular disorders, fibrosis in major organs, bone loss). Another goal of this pilot study is to investigate the state of disease against proposed medical countermeasures, in order to determine whether the countermeasures are efficacious in preventing or mitigating these injuries. An example of an up and coming tear biomarker technology, Ascendant Dx, a clinical stage diagnostic company, is developing a screening test to detect breast cancer using proteins from tears. The team utilized Liquid Chromatography -Mass Spectrometry with Mass analysis (LC MS/MS) as a discovery platform followed by validation with ELISA to come up with a panel of protein biomarkers that can differentiate breast cancer samples from control ("cancer free") samples with results far surpassing the results of imaging techniques in use today. Continued research into additional proteins is underway to increase the sensitivity and specificity of the test and development efforts are on the way to transfer the test onto a fast, accurate and inexpensive point of care platform. In conclusion, the expected results from this proposed pilot study are to: a) establish an SOP for retrieving/storing/transporting tear fluid samples from multicentre sites b) establish a normal range for relevant biomarkers in tears; and c) establish a database (biobank) of tears of space naïve versus veteran astronauts, to establish a personal baseline for long-term ocular health monitoring

Morton, Stephen↗
NASA NTRS2018

A Summary of Results from Two Full-Scale Fokker F28 Fuselage Section Drop Tests

During 2017, two vertical drop tests were conducted on two partial sections removed from a Fokker F28 MK4000 aircraft as a part of a joint NASA / Federal Aviation Administration (FAA) effort to investigate the crashworthiness characteristics of Transport Category Aircraft, as defined by 14 Code of Federal Regulations, Part 25. The first test was a pure vertical drop test of a relatively uniform forward section, which included an underfloor area for luggage. The second test was a canted drop test onto a sloped surface of a portion of the fuselage representing the wingbox stiffened structure. In both tests, accelerometers were installed on the floor, seat track, luggage, and overhead bin to measure responses in the two airframe sections. In addition, ten Anthropomorphic Test Devices (ATDs, a.k.a. crash test dummies) were included in each test to measure the potential of onboard occupant injury. Self-contained data recorders, logging accelerations and rotational rates, were also used on the seat tracks and lower structure for evaluation as potential crash recording devices in future tests. Finally, the starboard side of each section was painted with a stochastic black and white speckle pattern for use in full field photogrammetric imaging techniques. The results collected show notable differences in the Forward and Wingbox Section responses. The Forward Section floor accelerations showed a relatively uniform response of approximately 7 g throughout the impact event. This section exhibited large amounts of subfloor crushing, floor stiffener failures and seat deformation upon impact. These results are contrasted by the Wingbox Section accelerations, which showed large differences when comparing accelerations recorded from either the rear or the front portion of the section reaching peaks of 39 g in some locations. Additionally, the Wingbox Section test induced a forward motion caused from the rotation at impact. With the exception of the lower cavity, there was minimal deformation in the Wingbox Section, and ATD responses were consistently higher than those for the Forward Section. A complete set of results are presented for the airframe, seat, ATD, overhead bin, and subfloor regions for each section. The ATD results are compared to current injury criteria, and determinations will be made on the likelihood of injury. Additional results for cargo-hold stored luggage are presented in an attempt to provide a component-level characterization for better understanding of under-floor loading. Finally, a discussion of the relevance of the results for a proposed airframe level crashworthiness guideline are presented.

Littell, Justin D.↗
NASA NTRS2018

Full-Scale Drop Test of a Fokker F28 Wingbox Fuselage Section

During the summer of 2017, a vertical drop test was conducted on a partial section of a Fokker F28 MK4000 aircraft as a part of a joint NASA/FAA effort to investigate the performance of transport category aircraft under realistic crash conditions. Ten Anthropomorphic Test Devices (ATDs, a.k.a. crash test dummies) ranging from 5th to 95th percentile sizes were used for the collection and comparison of occupant loads. Additionally, overhead bin mass simulators were added to achieve a realistic fuselage configuration. The section was dropped with a downward facing pitch angle onto a sloping soil surface in order to simulate a local horizontal velocity in the airframe. Instrumentation consisting of accelerometers was installed to measure floor, seat track, ATD, and overhead bin acceleration responses. Self-contained data recorders logging accelerations and rotational rates were also used on the seat tracks and lower structure as evaluations for crash recording devices in potential future use cases. The right side of the section was painted with a stochastic black and white speckle pattern for use in full field photogrammetric imaging techniques. Results collected from the airframe accelerometers will be presented, and deformation and failures of the test article structure will be discussed. Finally, an examination of the test article motion will be presented using derived components of local velocities with their effect on the impact acceleration and airframe response.

Littell, J. D.↗
NASA NTRS2018

Observations of Fast-Moving Features in the Debris Disk of AU Mic on a Three-Year Timescale: Confirmation and New Discoveries

Context. The nearby and young M star AU Mic is surrounded by a debris disk in which we previously identified a series of large-scale arch-like structures that have never been seen before in any other debris disk and that move outward at high velocities. Aims. We initiated a monitoring program with the following objectives: (1) track the location of the structures and better constrain their projected speeds, (2) search for new features emerging closer in, and ultimately (3) understand the mechanism responsible for the motion and production of the disk features. Methods. AU Mic was observed at 11 different epochs between August 2014 and October 2017 with the IR camera and spectrograph of SPHERE. These high-contrast imaging data were processed with a variety of angular, spectral, and polarimetric differential imaging techniques to reveal the faintest structures in the disk. We measured the projected separations of the features in a systematic way for all epochs. We also applied the very same measurements to older observations from the Hubble Space Telescope (HST) with the visible cameras STIS and ACS. Results. The main outcomes of this work are (1) the recovery of the five southeastern broad arch-like structures we identified in our first study, and confirmation of their fast motion (projected speed in the range 4–12 km/s); (2) the confirmation that the very first structures observed in 2004 with ACS are indeed connected to those observed later with STIS and now SPHERE; (3) the discovery of two new very compact structures at the northwest side of the disk (at 0.40′′ and 0.55′′ in May 2015) that move to the southeast at low speed; and (4) the identification of a new arch-like structure that might be emerging at the southeast side at about 0.4′′ from the star (as of May 2016). Conclusions. Although the exquisite sensitivity of SPHERE allows one to follow the evolution not only of the projected separation, but also of the specific morphology of each individual feature, it remains difficult to distinguish between possible dynamical scenarios that may explain the observations. Understanding the exact origin of these features, the way they are generated, and their evolution over time is certainly a significant challenge in the context of planetary system formation around M stars.

Boccaletti, A.↗
NASA NTRS2019

New Technologies for Enabling Food Production Beyond LEO

NASA has identified the need for robust and sustainable ‘Pick-and-Eat’ systems for supplementing crew diets with fresh leafy green crops in near-term LEO (Low Earth Orbit), cislunar, and lunar missions. Spaceflight plant growth systems have been primarily designed for conducting space biology studies, but these systems are not optimal for sustained food production. Improved water and nutrient delivery subsystems that do not use bulky and non-reusable media are needed for decreasing the mass of the food production system. Autonomous technologies for monitoring plant health and food safety are needed for ensuring that the food produced is suitable supplementing crew diets with fresh, nutritious salad crops. Improved plant imaging techniques used for high-throughput phenotyping can be leveraged for monitoring plant health. Near-real-time measurements of the microbial ecology of food production systems are needed for assessing food safety. Furthermore, newly identified plant species and cultivars with improved growth habits and contents of antioxidants, vitamins, and minerals when grown in spaceflight environmental conditions are needed. These improvements in food production technologies will enable the design of sustainable life support systems for manned exploration missions beyond Low Earth Orbit.

Monje Mejia, Oscar A.↗
NASA NTRS2013

TPSAS-NF1676L-17553-DND

Investigate the potential of using an efficient damage imaging technique for damage identification by using a commercial finite element code to simulate the Lamb wave propagation in stiffened composite structures.

J T Wang↗
NASA NTRS1993

Below Band-Gap Laser Ablation of Diamond for Transmission Electron Microscopy

A 248-nm excimer laser was used to thin naturally occurring type 1a diamond substrates at normal and glancing (22 deg) incidence. Perforation of a 250 micron thick substrate was achieved in about 15 minutes at normal incidence. Whilst the substrate thinned at glancing incidence was found to have large electron-transparent areas, that thinned at normal incidence required additional argon milling to achieve electron transparency. X-ray photoelectron spectroscopy of the back surface of the diamond failed to detect any graphite or glassy carbon, confirming that damage due to laser ablation occurs only at the incident surface. Samples prepared using this technique imaged in the transmission electron microscope were observed to have retained the nitrogen platelets

Posthill, J. B.↗
NASA NTRS2021

Keck/OSIRIS Paβ High-contrast Imaging and Updated Constraints on PDS 70b

We present a high-contrast imaging search for Paβ line emission from protoplanets in the PDS 70 system with Keck/OSIRIS integral field spectroscopy. We applied the high-resolution spectral differential imaging technique to the OSIRIS J-band data but did not detect the Paβ line at the level predicted using the parameters of Hashimoto et al. (2020). This lack of Paβ emission suggests the MUSE-based study may have overestimated the line width of Hα. We compared our Paβ detection limits with the previous Hα flux and Hβ limits and estimated AV to be ∼0.9 and 2.0 for PDS 70 b and c, respectively. In particular, PDS 70 b’s AV is much smaller than implied by high-contrast near-infrared studies, which suggests the infrared-continuum photosphere and the hydrogen-emitting regions exist at different heights above the forming planet.

Taichi Uyama↗
NASA NTRS2022

Compact Midwave Imaging System: Results from an Airborne Demonstration

The Compact Midwave Imaging System (CMIS) is a wide field of view, multi-angle, multi-spectral push frame imager that relies on the forward motion of the satellite to create a two-dimensional (2D) image swath. An airborne demonstration of CMIS was successfully completed in January-February 2021 on the NASA Langley Research Center Gulfstream III. The primary objective of the four-flight campaign was to demonstrate the capability of this unique instrument to perform stereo observations of clouds and other particulates (e.g. smoke) in the atmosphere. It is shown that the midwave infrared (MWIR) spectral bands of CMIS provide a unique 24/7 capability with high resolution for accurate stereo sensing.The instrument relies on new focal plane array (FPA) technology, which provides excellent sensitivity at much warmer detector temperatures than traditional technologies. This capability enabled a compact, low-cost design that can provide atmospheric motion vectors and cloud heights to support requirements for atmospheric winds in the 2017-2027 Earth Science Decadal Survey. Applications include day/night observations of the planetary boundary layer, severe weather, and wildfires. A comparison with current space-based earth science instruments demonstrates that the SWIR/MWIR multi-spectral capability of CMIS is competitive with larger, more expensive instrumentation. Imagery obtained over a controlled burn and operating nuclear power plant demonstrated the sensitivity of the instrument to temperature variations. The system relies on a mature stereoscopic imaging technique applied to the same scene from two independent platforms to unambiguously retrieve atmospheric motion vectors (AMVs) with accurate height assignment.This capability has been successfully applied to geostationary and low-earth orbit satellites to achieve excellent accuracy. When applied to a ground-point validation case, the accuracy for the CMIS aircraft observations was 20 m and 0.3 m/s for cloud heights and motion vectors, respectively. This result was confirmed by a detailed error analysis with analytical and covariance models. The results for CMIS cases with under flights of Aeolus, CALIPSO and Aqua provided a good validation of expected accuracies. The paper also showed the feasibility of accommodating CMIS on CubeSats to enable multiple instruments to be flown in a leader-follower mode.

stereo winds↗
NASA NTRSDate not supplied

Design and Testing of a Device for Homogeneous Moon Dust Deposition on Test Articles/Systems

During this decade, NASA is charged to return humans to the surface of the Moon. One of the most challenging aspects to operating on the lunar surface is the regolith present, which is pervasive and damaging. To support systems design and validation for this environment, the handling and application of lunar simulants onto test articles or systems becomes important, albeit difficult. A device to apply the same amount of dust uniformly was developed in a previous study (Hollingsworth et al., 2006) but was only utilized with Mars dust simulant during that previous project. The current work provides evaluation of this device with multiple lunar simulants, each representing different composition as found at various locations on the Moon’s surface. Enhancements to the design and operation of the dust distributor are also described, along with a new digital imaging technique to calculate the percent surface coverage of the dust after deposition. The results support the use of the distributor and improved techniques to provide a uniform coating of dust on test articles and systems.

lunar↗
NASA NTRSDate not supplied

Design and Testing of a Device for Moon Dust Deposition on Test Articles/Systems

During this decade, NASA is charged to return humans to the surface of the Moon. One of the most challenging aspects to operating on the lunar surface is the regolith present, which is pervasive and damaging. To support systems design and validation for this environment, the handling and application of lunar simulants onto test articles or systems becomes important, albeit difficult. A device to apply the same amount of dust uniformly was developed in a previous study but was only utilized with Mars dust simulant during that previous project. The current work provides evaluation of this device with multiple lunar simulants, each representing different composition as found at various locations on the Moon’s surface. Enhancements to the design and operation of the dust distributor are also described, along with a new digital imaging technique to calculate the percent surface coverage of the dust after deposition. The results support the use of the distributor and improved techniques to provide a uniform coating of dust on test articles and systems.

Kathryn M Hurlbert↗
NASA NTRSDate not supplied

The Porous Microstructure Analysis (PuMA) software

The open-source Porous Microstructure Analysis (PuMA) software was implemented to offer an efficient framework for determining material characteristics from 3D microstructures. Its development was inspired by progress in X-ray microtomography, an imaging technology that captures the internal structure of materials in 3D, and even in a 4D temporal context. Over recent years, this method has transformed the domain of materials science due to its capability to non-destructively examine material microstructures while presenting digital data about their geometrical details. It has provided insights into materials relevant to several NASA missions, including heatshields, parachute fabrics, meteorites, and other advanced composites. PuMA, in its current version 3, delivers an array of features, spanning from basic geometric insights of a microstructure to intricate anisotropic thermo-elastic and chemical behavior. Specifically, the software evaluates morphological attributes (specific surface area, volume fractions, mean intercept lengths, orientation) and physical characteristics (conductivity, elasticity, permeability, and tortuosity). Additionally, it can model material degradation processes, such as oxidation and surface chemistry interactions. The software can generate synthetic microstructures, from straightforward geometrical designs to intricate woven and non-woven geometries. Coupling material generation and characterization enable parametric studies and sensitivity analysis to optimize the microstructural performance and inform design decisions and reliability assessment based on uncertainty quantification. A recent addition to PuMA includes the TomoSAM plugin, devised to incorporate the cutting-edge Segment Anything Model (SAM). SAM is a promptable deep learning model that can identify objects and create image masks in a zero-shot manner, based only on a few user clicks. The synergy between these tools aids in the segmentation of complex 3D datasets from tomography or other imaging techniques, which would otherwise require a laborious manual segmentation process.

Tomography↗
NASA NTRSDate not supplied

The Porous Microstructure Analysis (PuMA) software

The open-source Porous Microstructure Analysis (PuMA) software was created to offer an efficient framework for determining material properties from 3D microstructures. Its development was inspired by progress in X-ray microtomography, an imaging technology that captures the internal structure of materials in 3D, and even in a 4D temporal context. Over recent years, this method has transformed the domain of materials science due to its capability to non-destructively examine material microstructures while presenting digital data about their geometrical details. It has provided insights into materials relevant to several NASA missions, including heatshields, parachute fabrics, meteorites, and other advanced composites. PuMA, in its current version 3, delivers an array of features, spanning from basic geometric insights of a microstructure to intricate anisotropic thermo-elastic and chemical behavior. Specifically, the software evaluates morphological attributes (specific surface area, volume fractions, mean intercept lengths, orientation) and physical characteristics (conductivity, elasticity, permeability, and tortuosity). Additionally, it can model material degradation processes, such as oxidation and surface chemistry interactions. The software can generate synthetic microstructures, from straightforward geometrical designs to intricate woven and non-woven geometries. Coupling material generation and characterization enables parametric studies and sensitivity analysis to optimize the microstructural performance and inform design decisions and reliability assessment based on uncertainty quantification. A recent addition to PuMA includes the TomoSAM plugin, devised to incorporate the cutting-edge Segment Anything Model (SAM) into our image segmentation workflow. SAM is a promptable deep learning model that can identify objects and create image masks in a zero-shot manner, based only on a few user clicks. The synergy between these tools aids in the segmentation of complex 3D datasets from tomography and other imaging techniques, which would otherwise require a laborious manual segmentation process.

Tomography↗
DOE OSTI2011

Enrichment Determination of Uranium in Shielded Configurations

The determination of the enrichment of uranium is required in many safeguards and security applications. Typical methods of determining the enrichment rely on detecting the 186 keV gamma ray emitted by 235 U. In some applications, the uranium is surrounded by external shields, and removal of the shields is undesirable. In these situations, methods relying on the detection of the 186 keV gamma fail because the gamma ray is shielded easily. Oak Ridge National Laboratory (ORNL) has previously measured the enrichment of shielded uranium metal using active neutron interrogation. The method consists of measuring the time distribution of fast neutrons from induced fissions with large plastic scintillator detectors. To determine the enrichment, the measurements are compared to a calibration surface that is created from Monte Carlo simulations where the enrichment in the models is varied. In previous measurements, the geometry was always known. ORNL is extending this method to situations where the geometry and materials present are not known in advance. In the new method, the interrogating neutrons are both time and directionally tagged, and an array of small plastic scintillators measures the uncollided interrogating neutrons. Therefore, the attenuation through the item along many different paths is known. By applying image reconstruction techniques, an image of the item is created which shows the position-dependent attenuation. The image permits estimating the geometry and materials present, and these estimates are used as input for the Monte Carlo simulations. As before, simulations predict the time distribution of induced fission neutrons for different enrichments. Matching the measured time distribution to the closest prediction from the simulations provides an estimate of the enrichment. This presentation discusses the method and provides results from recent simulations that show the importance of knowing the geometry and materials from the imaging system.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2012

Determination of elemental impurities in plastic calibration standards using laser induced breakdown spectroscopy

Dual-energy computed tomography (CT) scanning is a rapidly emerging imaging technique employed in nondestructive evaluation of various materials. CT has been used for characterizing rocks and visualizing multiphase flow through rocks for over 25 years. The most common technique for dual-energy CT scanning relies on homogeneous calibration standards to produce the most accurate decoupled data. However, the use of calibration standards with impurities increases the probability of error in the reconstructed data and results in poor rock characterization. Laser-induced breakdown spectroscopy was used to determine impurity concentration in a set of commercially purchased calibration standards used in dual-energy scanning for material identification with coal samples. Two calibration models were developed by using univariate calibration with the internal ratio method and multiple linear regression. Seven elements (Al, Fe, Mg, Na, Ni, Sr, and Ti) were examined in five different samples containing varying amounts of each ion to compare calibration from univariate data analysis and from multivariate data analysis. The contaminant concentrations were also measured by a commercially available inductively coupled plasma optical emission spectroscopy instrument, and the data were used as a reference in developing calibration curves for a modified version of the single linear regression model and the multiple linear regression model.

01 COAL, LIGNITE, AND PEAT↗
DOE OSTI2012

Direction-Sensitive Hand-Held Gamma-Ray Spectrometer

A novel, light-weight, hand-held gamma-ray detector with directional sensitivity is being designed. The detector uses a set of multiple rings around two cylindrical surfaces, which provides precise location of two interaction points on two concentric cylindrical planes, wherefrom the source location can be traced back by back projection and/or Compton imaging technique. The detectors are 2.0 × 2.0 mm europium-doped strontium iodide (SrI2:Eu2+) crystals, whose light output has been measured to exceed 120,000 photons/MeV, making it one of the brightest scintillators in existence. The crystal’s energy resolution, less than 3% at 662 keV, is also excellent, and the response is highly linear over a wide range of gamma-ray energies. The emission of SrI2:Eu2+ is well matched to both photo-multiplier tubes and blue-enhanced silicon photodiodes. The solid-state photomultipliers used in this design (each 2.0 × 2.0 mm) are arrays of active pixel sensors (avalanche photodiodes driven beyond their breakdown voltage in reverse bias); each pixel acts as a binary photon detector, and their summed output is an analog representation of the total photon energy, while the individual pixel accurately defines the point of interaction. A simple back-projection algorithm involving cone-surface mapping is being modeled. The back projection for an event cone is a conical surface defining the possible location of the source. The cone axis is the straight line passing through the first and second interaction points.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2013

Kinetic instabilities and turbulence in photonic plasma

The purpose of this proposal was to study the statistical dynamics of plasma and demonstrate it experimentally in all-optical systems. That this was possible followed from (1) a direct mapping between the equations of plasma kinetics and the nonlinear propagation of statistical light and (2) advances in photonic technology, which enabled versatile control of the input conditions and direct imaging of the output fields. At the start of the proposal, theory exploiting the optics plasma relationship was minimal, and few experiments had been performed. By the end, the field of photonic plasma had been firmly established. There were two main themes in the proposal. The first was optical confirmation of existing plasma theory, particularly where competing theories or numerical codes needed to be distinguished. The second was the genesis of new research directions for plasma, both fundamentally and for applications. These arose naturally from the proposed projects, as experiment raises different questions than theory, while optics gives a much different perspective than material science. The photonic analogues also provide a unified language for many issues that are difficult (and expensive) to study directly, such as high-energy laser-plasma interactions. Moreover, basic imaging techniques enable measurements that are difficult, if not impossible, in material plasma. For example, Debye shielding hides internal dynamics from outside detectors while nonlinear interactions must often be inferred by deviations from linear expectations. In contrast, optics allows the creation of a wide range of spatial and spectral distributions, along with high-resolution measurements in the full position-momentum phase space.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2014

Revealing the Differences Between Free and Complexed Enzyme Mechanisms and Factors Contributing to Cell Wall Recalcitrance

Enzymatic depolymerization of polysaccharides is a key step in the production of fuels and chemicals from lignocellulosic biomass, and discovery of synergistic biomass-degrading enzyme paradigms will enable improved conversion processes. Historically, revealing insights into enzymatic saccharification mechanisms on plant cell walls has been hindered by uncharacterized substrates and low resolution imaging techniques. Also, translating findings between model substrates to intact biomass is critical for evaluating enzyme performance. Here we employ a fungal free enzyme cocktail, a complexed cellulosomal system, and a combination of the two to investigate saccharification mechanisms on cellulose I, II and III along with corn stover from Clean Fractionation (CF), which is an Organosolv pretreatment. The insoluble Cellulose Enriched Fraction (CEF) from CF contains mainly cellulose with minor amounts of residual hemicellulose and lignin, the amount of which depends on the CF pretreatment severity. Enzymatic digestions at both low and high-solids loadings demonstrate that CF reduces the amount of enzyme required to depolymerize polysaccharides relative to deacetylated, dilute acid pretreated corn stover. Transmission and scanning electron microscopy of the biomass provides evidence for the different mechanisms of enzymatic deconstruction between free and complexed enzyme systems, and reveals the basis for the synergistic relationship between the two enzyme paradigms on a process-relevant substrate for the first time. These results also demonstrate that the presence of lignin, rather than cellulose morphology, is more detrimental to cellulosome action than to free cellulases. As enzyme costs are a major economic driver for biorefineries, this study provides key inputs for the evaluation of CF as a pretreatment method for biomass conversion.

09 BIOMASS FUELS↗