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NASA NTRS2002

Physics of Colloids in Space: Microgravity Experiment Launched, Installed, and Activated on the International Space Station

The Physics of Colloids in Space (PCS) experiment is a Microgravity Fluids Physics investigation that is presently located in an Expedite the Process of Experiments to Space Station (EXPRESS) Rack on the International Space Station. PCS was launched to the International Space Station on April 19, 2001, activated on May 31, 2001, and will continue to operate about 90 hr per week through May 2002.

Doherty, Michael P.↗
NASA NTRS2005

STS-114: Discovery Q and A with Joint Crew on ISS

Commander Sergei Krikalev and Flight Engineer John Phillips of the Expedition 11 crew, along with the STS-114 Discovery crewmembers are shown on the International Space Station, answering questions from the news media. Eileen Collins, Commander summarizes the untold story of this mission and she also describes what re-entry is like. Steven Robinson, Mission Specialist talks about Extravehicular Activity (EVA) and the gap fillers used to replace missing tiles on the Space Shuttle Discovery. John Phillips describes the equipment coming off the space station and how this equipment will make the International Space Station more efficient. Soichi Noguchi, Mission Specialist answers questions about EVA 1, 2, and 3 and Collins talks about what impressed her most during these EVA's.

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NASA NTRS2005

Atmospheric Hydroperoxides in West Antarctica: Links to Stratospheric Ozone and Atmospheric Oxidation Capacity

The troposphere above the West Antarctic Ice Sheet (WAIS) was sampled for hydroperoxides at 21 locations during 2-month-long summer traverses from 2000 to 2002, as part of US ITASE (International Transantarctic Scientific Expedition). First time quantitative measurements using an HPLC method showed that methylhydroperoxide (MHP) is the only important organic hydroperoxide occurring in the Antarctic troposphere, and that it is found at levels ten times those previously predicted by photochemical models. During three field seasons, means and standard deviations for hydrogen peroxide (H2O2) were 321+/-158 pptv, 650+/-176 pptv and 330+/-147 pptv. While MHP was detected, but not quantified in December 2000, levels in summer 2001 and 2002 were 317+128 pptv and 304+/-172.2 pptv. Results from firn air experiments and diurnal variability of the two species showed that atmospheric H2O2 is significantly impacted by a physical snow pack source between 76 and 90degS, whereas MHP is not. We show strong evidence of a positive feedback between stratospheric ozone and H2O2 at the surface. Between November-27 and December-12 in 2001, when ozone column densities dropped below 220 DU (means in 2000 and 2001 were 318 DU and 334 DU, respectively), H2O2 was 1.7 times that observed in the same period in 2000 and 2002, while MHP was only 80% of the levels encountered in 2002. Photochemical box model runs suggest that NO and OH levels on WAIS are closer to coastal values, while Antarctic Plateau levels are higher, confirming that region to be a highly oxidizing environment. The modeled sensitivity of H2O2 and particularly MHP to NO offers the potential to use atmospheric hydroperoxides to constrain the NO background and thus estimate the past oxidation capacity of the remote atmosphere. Index Terms: 0365 Atmospheric Composition and Structure: Troposphere: composition and chemistry; 0322 Atmospheric Composition and Structure: Constituent sources and sinks; 1610 Global Change: Atmosphere (03 15,0325); 0736 Cryosphere: Snow (1827, 1863); 0724 Cryosphere: Ice Cores (4932) Keywords: hydrogen peroxide, methylhydroperoxide, Antarctica, air-snow exchange, stratospheric ozone, atmospheric oxidation capacity

Frey, Markus M.↗
NASA NTRS2002

Low Temperature Reliability of Electronic Packages/Assemblies for Space Missions

A NASA-wide team, funded under the NASA Electronic Parts and Packaging Program (NEPP), was formed to collaborate and to establish reliability of various electronic parts/packaging and assemblies for operation under extreme cold temperatures. One of the primary objectives of the NEPP is to expedite the infusion of cutting edge technologies into the present and future NASA missions. Commercial-off-the-shelf (COTS) emerging electronic parts/packages due to their lower weight, increased functionality, and lower cost are excellent candidates for space missions if they are characterized to show that they will meet the stringent reliability and quality requirements. Characterizations, especially for the extreme cold temperatures, are required since very limited data are available by manufacturers or users. For severe military environments, the temperature conditions to -65 C are the lowest temperature for which these parts/packages and assemblies are qualified. New data beyond this relatively benign cold temperature are required for numerous NASA missions. Several parts/packages, based on the project recommendation for their immediate and future needs, were selected for detailed characterization to cold temperature regimes down to liquid nitrogen (-196 C), covering both Mars cold temperature (-125 C) and asteroid (- 180 C) lander environmental requirements. Numerous parts/packages and assemblies were characterized during extreme temperature environmental tests. Several electrical parameters were characterized at discrete temperatures to -185 C to determine if they remain within their specification ranges. Both packages and circuit boards were subjected to nondestructive testing including optical, X-ray, and acoustic microscopy to document their integrity prior to environment exposure. Package/board assemblies were also subjected to X-ray to characterize solder joint integrity including void levels. Both parts and assemblies were subjected to thermal cycling with a large temperature swing enveloping numerous NASA missions. Details of the performed tests and the results obtained are presented.

Patterson, Richard L.↗
NASA NTRS2005

STS-114: Discovery Question & Answer with Joint Crew on ISS

STS-114 Commander Eileen Collins, Pilot James Kelly, Mission Specialists Souichi Noguchi, Stephen Robinson, Charles Camarda, Andrew Thomas, Wendy Lawrence, and Expedition 11 Commander Sergei Krikalev and Flight Engineer John Phillips answers questions from United States, Japanese and Russian News media in the Destiny laboratory of the International Space Station. Risk, safety, extravehicular activities, spacewalks, re-entry, gap fillers, tiles, flight operations, flight crew activities, team work, and life in space are topics covered with the News media.

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NASA NTRS2003

Integrated Propulsion/Vehicle System Structurally Optimized

Ongoing research and testing are essential in the development of air-breathing hypersonic propulsion technology, and this year some positive advancement was made at the NASA Glenn Research Center. Recent work performed for GTX, a rocket-based combined-cycle, single-stage-to-orbit concept, included structural assessments of both the engine and flight vehicle. In the development of air-breathing engine technology, it is impractical to design and optimize components apart from the fully integrated system because tradeoffs must be made between performance and structural capability. Efforts were made to control the flight trajectory, for example, to minimize the aerodynamic heating effects. Structural optimization was applied to evaluate concept feasibility and was instrumental in the determination of the gross liftoff weight of the integrated system. Achieving low Earth orbit with even a small payload requires an aggressive approach to weight minimization through the use of lightweight, oxidation-resistant composite materials. Assessing the integrated system involved investigating the flight trajectory to determine where the critical load events occur in flight and then generating the corresponding environment at each of these events. Structural evaluation requires the mapping of the critical flight loads to finite element models, including the combined effects of aerodynamic, inertial, combustion, and other loads. NASA s APAS code was used to generate aerodynamic pressure and temperature profiles at each critical event. The radiation equilibrium surface temperatures from APAS were used to predict temperatures through the thickness. Heat transfer solutions using NASA's MINIVER code and the SINDA code (Cullimore & Ring Technologies, Littleton, CO) were calculated at selective points external to the integrated vehicle system and then extrapolated over the entire exposed surface. FORTRAN codes were written to expedite the finite element mapping of the aerodynamic heating effects for the internal structure.

Hunter, James E.↗
NASA NTRS2005

STS-114: Discovery Day 3 Mission Status Briefing

Paul Hill, STS-114 Lead Shuttle Flight Director and John Shannon, Space Shuttle Flight Operations and Integration Manager are seen in this STS-114 flight day three Mission Status Briefing. Hill expresses that the Discovery Orbiter is performing beautifully and that STS-114 crew performed a picture perfect rendezvous. He also shows a video of the Rendezvous Pitch Maneuver (RPM) where Commander Eileen Collins slowly flips Discovery in a circle to give the Expedition 11 crew a view of Discovery's heat resistant tiles. He talks about the removal of the Multi-Purpose Logistics Module (MPLM) from Discovery's payload bay using the ISS Robotic Arm. Shannon discusses two objectives that his team is fulfilling on this mission. They include: 1) Understanding the health of Space Shuttle Discovery; and 2) Assessing the performance of the External Tank. Questions from the news media pertaining to the life expectancy of the Space Shuttle Discovery, battery lifetime on the wing leading edge impact sensors, foam loss, PAL ramps, and the safety of the Space Shuttle Discovery are addressed.

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NASA NTRS2004

Physics of Colloids in Space--Plus (PCS+) Experiment Completed Flight Acceptance Testing

The Physics of Colloids in Space--Plus (PCS+) experiment successfully completed system-level flight acceptance testing in the fall of 2003. This testing included electromagnetic interference (EMI) testing, vibration testing, and thermal testing. PCS+, an Expedite the Process of Experiments to Space Station (EXPRESS) Rack payload will deploy a second set of colloid samples within the PCS flight hardware system that flew on the International Space Station (ISS) from April 2001 to June 2002. PCS+ is slated to return to the ISS in late 2004 or early 2005.

Doherty, Michael P.↗
NASA NTRS2004

Human Missions to Europa and Titan - Why Not?

This report describes a long-term development plan to enable human exploration of the outer solar system, with a focus on Europa and Titan. These are two of the most interesting moons of Jupiter and Saturn, respectively, because they are the places in the solar system with the greatest potential for harboring extraterrestrial life. Since human expeditions to these worlds are considered impossible with current capabilities, the proposal of a well-organized sequence of steps towards making this a reality was formulated. The proposed Development Plan, entitled Theseus, is the outcome of a recent multinational study by a group of students in the framework of the Master of Space Studies (MSS) 2004 course at the International Space University (ISU). The Theseus Program includes the necessary development strategies in key scientific and technological areas that are essential for identifying the requirements for the exploration of the outer planetary moons. Some of the topics that are analysed throughout the plan include: scientific observations at Europa and Titan, advanced propulsion and nuclear power systems, in-situ resource utilization, radiation mitigation techniques, closed life support systems, habitation for long-term spaceflight, and artificial gravity. In addition to the scientific and technological aspects of the Theseus Program, it was recognized that before any research and development work may begin, some level of program management must be established. Within this chapter, legal issues, national and international policy, motivation, organization and management, economic considerations, outreach, education, ethics, and social implications are all considered with respect to four possible future scenarios which enable human missions to the outer solar system. The final chapter of the report builds upon the foundations set by Theseus through a case study. This study illustrates how such accomplishments could influence a mission to Europa to search for evidence of life in its subsurface oceans. The future remains unpredictable, as does the realization of any of these possibilities. However, projects such as this remind us that the final frontier for humans is truly outer space, and only our imagination will determine where the frontier stops. We can dream of visiting other planetary systems and perhaps even galaxies, but we must begin closer, and considering the scope of our known universe, Europa and Titan are very close indeed.

FROM↗
NASA NTRS2004

Training Space Surgeons for Missions to the Moon and Mars

Over a period of 4 years, several working groups reviewed the provisions for medical care in low earth orbit and for future flights such as to the Moon and Mars. More than 60 medical experts representing a wide variety of clinical backgrounds participated in the working groups. They concluded that NASA medical training for long-duration missions, while critical to success, is currently aimed at short-term skill retention. They noted that several studies have shown that skills and knowledge deteriorate rapidly in the absence of adequate sustainment training. American Heart Association studies have shown that typically less than twenty-five percent of learned skills remain after 6 to 8 months. In addition to identifying the current training deficiencies, the working groups identified additional skill and knowledge sets required for missions to the Moon and Mars and curricula were developed to address inadequacies. Space medicine care providers may be categorized into 4 types based on health care responsibilities and level of education required. The first 2 types are currently recognized positions within the flight crew: crew medical officers and astronaut-physician. The crew medical officer (CMO), a non-medically trained astronaut crewmember, is given limited emergency medical technician-like training to provide medical care on orbit. Many of hidher duties are carried out under the direction of a ground-based flight surgeon in mission control. Second is the astronaut- physician whose primary focus is on mission specialist duties and training, and who has very limited ability to maintain medical proficiency. Two new categories are recommended to complete the 4 types of care providers primarily to address the needs of those who will travel to the Moon and Mars. Physician astronaut - a physician, who in addition to being a mission specialist, will be required to maintain and enhance hidher medical proficiency while serving as an astronaut. Space surgeon - a physician astronaut given special training to address the unique health care requirements envisioned for expeditions such as those to Mars.

Pool, S. L.↗
NASA NTRS2005

Pre-Launch GOES-R Risk Reduction Activities for the Geostationary Lightning Mapper

The GOES-R Geostationary Lightning Mapper (GLM) is a new instrument planned for GOES-R that will greatly improve storm hazard nowcasting and increase warning lead time day and night. Daytime detection of lightning is a particularly significant technological advance given the fact that the solar illuminated cloud-top signal can exceed the intensity of the lightning signal by a factor of one hundred. Our approach is detailed across three broad themes which include: Data Processing Algorithm Readiness, Forecast Applications, and Radiance Data Mining. These themes address how the data will be processed and distributed, and the algorithms and models for developing, producing, and using the data products. These pre-launch risk reduction activities will accelerate the operational and research use of the GLM data once GOES-R begins on-orbit operations. The GLM will provide unprecedented capabilities for tracking thunderstorms and earlier warning of impending severe and hazardous weather threats. By providing direct information on lightning initiation, propagation, extent, and rate, the GLM will also capture the updraft dynamics and life cycle of convective storms, as well as internal ice precipitation processes. The GLM provides information directly from the heart of the thunderstorm as opposed to cloud-top only. Nowcasting applications enabled by the GLM data will expedite the warning and response time of emergency management systems, improve the dispatch of electric power utility repair crews, and improve airline routing around thunderstorms thereby improving safety and efficiency, saving fuel and reducing delays. The use of GLM data will assist the Bureau of Land Management (BLM) and the Forest Service in quickly detecting lightning ground strikes that have a high probability of causing fires. Finally, GLM data will help assess the role of thunderstorms and deep convection in global climate, and will improve regional air quality and global chemistry/climate modeling. The GLM has a robust design that benefits and improves upon its strong heritage of NASA-developed LEO predecessors, the Optical Transient Detector (OTD) and the Lightning Imaging Sensor (LIS). GLM will have a substantially larger number of pixels within the focal plane, two lens systems, and multiple Real-Time Event Processors REPS for on-board event detection and data compression to provide continuous observations of the Americas and adjacent oceans.

Goodman, S. J.↗
NASA NTRS2006

EV13 Genesis Reentry Observations and Data Analysis

The Genesis spacecraft reentry represented a unique opportunity to observe a "calibrated meteor" from northern Nevada. Knowing its speed, mass, composition, and precise trajectory made it a good subject to test some of the algorithms used to determine meteoroid mass from observed brightness. It was also a good test of an inexpensive set of cameras which could be deployed to observe future shuttle reentries. The utility of consumer grade video cameras was evident during the STS-107 accident investigation and the Genesis reentry gave us the opportunity to specify and test commercially available cameras which could be used during future reentries. This report describes the video observations and their analysis, compares the results with a simple photometric model, describes the forward scatter radar experiment, and lists lessons learned from the expedition and implications for the Stardust reentry in January 2006 as well as future shuttle reentries.

Swift, Wesley R.↗
NASA NTRS2005

Cognitive Functioning in Space Exploration Missions: A Human Requirement

Solving cognitive issues in the exploration missions will require implementing results from both Human Behavior and Performance, and Space Human Factors Engineering. Operational and research cognitive requirements need to reflect a coordinated management approach with appropriate oversight and guidance from NASA headquarters. First, this paper will discuss one proposed management method that would combine the resources of Space Medicine and Space Human Factors Engineering at JSC, other NASA agencies, the National Space Biomedical Research Institute, Wyle Labs, and other academic or industrial partners. The proposed management is based on a Human Centered Design that advocates full acceptance of the human as a system equal to other systems. Like other systems, the human is a system with many subsystems, each of which has strengths and limitations. Second, this paper will suggest ways to inform exploration policy about what is needed for optimal cognitive functioning of the astronaut crew, as well as requirements to ensure necessary assessment and intervention strategies for the human system if human limitations are reached. Assessment strategies will include clinical evaluation and fitness-to-perform evaluations. Clinical intervention tools and procedures will be available to the astronaut and space flight physician. Cognitive performance will be supported through systematic function allocation, task design, training, and scheduling. Human factors requirements and guidelines will lead to well-designed information displays and retrieval systems that reduce crew time and errors. Means of capturing process, design, and operational requirements to ensure crew performance will be discussed. Third, this paper will describe the current plan of action, and future challenges to be resolved before a lunar or Mars expedition. The presentation will include a proposed management plan for research, involvement of various organizations, and a timetable of deliverables.

Fiedler, Edan↗
NASA NTRS2006

Bioinformatics for Exploration

For the purpose of this paper, bioinformatics is defined as the application of computer technology to the management of biological information. It can be thought of as the science of developing computer databases and algorithms to facilitate and expedite biological research. This is a crosscutting capability that supports nearly all human health areas ranging from computational modeling, to pharmacodynamics research projects, to decision support systems within autonomous medical care. Bioinformatics serves to increase the efficiency and effectiveness of the life sciences research program. It provides data, information, and knowledge capture which further supports management of the bioastronautics research roadmap - identifying gaps that still remain and enabling the determination of which risks have been addressed.

Johnson, Kathy A.↗
NASA NTRS2005

DEVELOPMENT OF AN INFLIGHT COUNTERMEASURE TO MITIGATE POSTFLIGHT GAIT DYSFUNCTION

Following spaceflight crewmembers experience gait and postural instabilities due to inflight adaptive alterations in sensorimotor function. These changes can pose a risk to crew safety if nominal or emergency vehicle egress is required immediately following long-duration spaceflight. At present, no operational countermeasure is available to mitigate postflight locomotor disturbances. Therefore, the goal of this study is to develop an inflight training regimen that facilitates the recovery of locomotor function after long-duration spaceflight. The countermeasure we are developing is based on the concept of variable practice. During this type of training the subject gains experience producing the appropriate adaptive motor behavior under a variety of sensory conditions and response constraints. This countermeasure is built around current ISS treadmill exercise activities. Crewmembers will conduct their nominal inflight treadmill exercise while being exposed to variations in visual flow patterns. These variations will challenge the postural and locomotor systems repeatedly, thereby promoting adaptive reorganization in locomotor behavior. As a result of this training a subject learns to solve a class of motor problems, rather than a specific motor solution to one problem, Le., the subject learns response generalizability or the ability to "learn to learn" under a variety of environmental constraints. We anticipate that this training will accelerate recovery of postural and locomotor function during readaptation to gravitational environments following spaceflight facilitating neural adaptation to unit (Earth) and partial (Mars) gravity after long-duration spaceflight. The study calls for one group of subjects to perform the inflight treadmill training regimen while a control group of subjects performs only the nominal exercise procedures. Locomotor function in both groups is assessed before and after spaceflight using two tests of gait function: The Integrated Treadmill Locomotion Test (ITLT) and the Functional Mobility Test (FMT). The ITLT characterizes alterations in the integrated function of multiple sensorimotor subsystems responsible for the control of locomotion. This test calls for subjects to walk on a motorized treadmill while we assess changes in dynamic postural stability, head-trunk coordination, short-latency head stabilization responses, dynamic visual acuity, lower limb coordination strategies and gait cycle timing. To make these assessments we measure the following parameters while subjects walk on the treadmill: 1) full body 3-dimensional kinematics using a motion capture system (Motion Analysis Corp., Santa Rosa, CA); 2) the shock-wave transmitted from heel-strike to the head using triaxial accelerometers placed on the tibia and head (Entran, Fairfield, NJ); 3) vertical forces using an instrumented treadmill (Kistler Instrument Corp., Amherst, NY); 4) Dynamic visual acuity using Landolt Cs presented on a laptop computer located 4m from the eyes and 5) Gait cycle timing using foot-switches (Motion Lab Systems, Inc., Baton Rouge, LA) attached to the plantar surface of each shoe at the heel and toe. The FMT evaluates a subject's ability to perform challenging locomotor maneuvers similar to those encountered during an egress from a space vehicle. Subjects step over and duck under obstacles along with negotiating a series of pylons set up on a base of 10 cm thick medium density foam. The dependent measures for the FMT are time to complete the course and the number of obstacles touched. To date, we have collected pre and postflight locomotion data from Expeditions 5-9 who will serve as part of the control group for this study. Preliminary results comparing the recovery rates in gait control sub-systems obtained from the ITLT and FMT performance showed two recovery patterns: 1) a concordant recovery trend between gait control parameters and FMT performance indicating a restitution pattern of recovery and 2) gait controecovery that lagged recovery in FMT performance suggesting that improvement in locomotor function was attained through a pattern of substitution. These data suggest that recovery of postflight locomotor function may occur through adaptive mechanisms that lead to either restitution or substitution of function. Understanding the modes of postflight readaptation has implications for countermeasure development and testing and in astronaut postflight rehabilitation.

Bloomberg, J. J.↗
NASA NTRS2006

Mission Architecture Comparison for Human Lunar Exploration

The Vision for Space Exploration outlines a bold new national space exploration policy that holds as one of its primary objectives the extension of human presence outward into the Solar System, starting with a return to the Moon in preparation for the future exploration of Mars and beyond. The National Aeronautics and Space Administration is currently engaged in several preliminary analysis efforts in order to develop the requirements necessary for implementing this objective in a manner that is both sustainable and affordable. Such analyses investigate various operational concepts, or mission architectures , by which humans can best travel to the lunar surface, live and work there for increasing lengths of time, and then return to Earth. This paper reports on a trade study conducted in support of NASA s Exploration Systems Mission Directorate investigating the relative merits of three alternative lunar mission architecture strategies. The three architectures use for reference a lunar exploration campaign consisting of multiple 90-day expeditions to the Moon s polar regions, a strategy which was selected for its high perceived scientific and operational value. The first architecture discussed incorporates the lunar orbit rendezvous approach employed by the Apollo lunar exploration program. This concept has been adapted from Apollo to meet the particular demands of a long-stay polar exploration campaign while assuring the safe return of crew to Earth. Lunar orbit rendezvous is also used as the baseline against which the other alternate concepts are measured. The first such alternative, libration point rendezvous, utilizes the unique characteristics of the cislunar libration point instead of a low altitude lunar parking orbit as a rendezvous and staging node. Finally, a mission strategy which does not incorporate rendezvous after the crew ascends from the Moon is also studied. In this mission strategy, the crew returns directly to Earth from the lunar surface, and is thus referred to as direct return. Figures of merit in the areas of safety and mission success, mission effectiveness, extensibility, and affordability are used to evaluate and compare the lunar orbit rendezvous, libration point rendezvous, and direct return architectures, and this paper summarizes the results of those assessments.

Geffre, Jim↗
NASA NTRS2006

Inspiring the Next Generation: Student Experiments and Educational Activities on the International Space Station, 2000-2006

One important objective of NASA has always been to inspire the next generation. NASA and human space flight have a unique ability to capture the imaginations of both students and teachers. The presence of humans onboard the International Space Station (ISS) for more than five years now has provided a foundation for numerous educational activities aimed at capturing the interest and motivating study in the sciences, technology, engineering, and mathematics. Yet even before the Expedition 1 crew arrived at station in November 2000, experiments with student participation were being conducted onboard ISS in support of NASA missions. One of NASA's protein crystal growth experiments had been delivered to station by the shuttle Atlantis during STS-106 in September 2000 and was returned to Earth six weeks later aboard the shuttle Discovery during the STS-92 mission. From very early on it was recognized that students would have a strong interest in the ISS, and that this would provide a unique opportunity for them to get involved and participate in science and engineering projects on ISS. It should be noted that participation is not limited to U.S. students but involves the 16 International Partner countries and various other countries under special commercial agree

Thomas, Donald A.↗
NASA NTRS2004

Antigenic Protein In Microgravity-Grown Human Mixed Mullerian Tumor (LN1) Cells Preserved In RNA Stabilizing Agent

Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LNI) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered Trademark) Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark) software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens.

Hammond, Dianne K.↗