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Ecogenomics of groundwater viruses suggests niche differentiation linked to specific environmental tolerance. This Narrative contains data for the 261 ENIGMA isolates described in the paper. See Table S2 for a complete list.
The dynamics of microbial processes are difficult to study in natural soil, owing to the small spatial scales on which microorganisms operate and to the opacity and chemical complexity of the soil habitat. To circumvent these challenges, we have created a 3D-bioprinted habitat that mimics aspects of natural soil aggregates while providing a chemically defined and translucent alternative culturing method for soil microorganisms. Our Synthetic Soil Aggregates (SSAs) retain the porosity, permeability, and patchy resource distribution of natural soil aggregates—parameters that are expected to influence emergent microbial community interactions. We demonstrate the printability and viability of several different microorganisms within SSAs and show how the SSAs can be integrated into a multi-omics workflow for single SSA resolution genomics, metabolomics, proteomics, lipidomics, and biogeochemical assays. We study the impact of the structured habitat on the distribution of a model co-culture microbial community and find that it is significantly different from the spatial organization of the same community in liquid culture, indicating a potential for SSAs to reproduce naturally occurring emergent community phenotypes. The SSAs have the potential as a tool to help researchers quantify microbial scale processes in situ and achieve high-resolution data from the interplay between environmental properties and microbial ecology.
While industrial emissions research has historically focused on energy-intensive sectors like steel, cement, and chemicals, this study addresses a critical gap by examining barriers across all the manufacturing industry in the U.S. Sectors like food processing, retail, plastics, and transportation face unique challenges distinct from heavy industry, operating on thin margins with limited bargaining power while experiencing heightened consumer and stakeholder pressure for improved environmental responsibility. Through structured interview data collection process and using quantitative ratings and qualitative analysis, this research identifies and categorizes emission reduction barriers across four key themes: financial, technical, organizational, and regulatory. Unlike energy-intensive industries that may pursue hydrogen or carbon capture technologies, discrete manufacturing industry like automotive, electrical and electronics, and machine manufacturers typically focus on energy efficiency, electrification of thermal processes, and alternate fuel switching, solutions better aligned with their lower-temperature processes and distributed facility profiles. The study’s primary contribution lies in documenting specific barrier manifestations within organizations and identifying proven mitigation strategies that companies have successfully implemented or observed among peers.
The experiments, environmental impact, and applications of data collected by ATS are discussed. Data cover communications, navigation, meteorology, data collection (including data from small unattended remote stations such as buoys, seismology and hydrology monitors, etc.), geodesy, and scientific experiments to define the environment at synchronous orbit, and to monitor emissions from the sun.
The logistics requirements for the space station cargo, the initial buildup, and the 90 day resupply are presented, along with the conceptual selection for the orbiter crew accommodations and the GSS logistics system. Various module configurations are outlined; structural/mechanical, environmental, temperature, voice communication, and data bus subsystems are also reviewed. Ground operations and module prelaunch and launch operations are discussed, as well as logistics system interfaces for space shuttles and stations.
The overall program background, the various system concepts considered, and the rationale for the selected design are described. The concepts for each subsystem are also described and compared. Details are given for the requirements, boom configuration and dynamics, actuators, man/machine interface and control, visual system, control system, environmental control and life support, data processing, and materials.
Fungal-like cultures isolated before, during, and after the 90-day test from samples of space station simulator (SSS) atmosphere, surfaces, subsystem componets, and crew dermal sites were identified to genus. Out of the original 525 isolates, approximately 80% were classified as bacteria. Laboratory methods (culture media, moisturization, and incubation temperatures) favored the recovery of medically significant bacteria rather than fungi. Therefore, fungal isolates were mostly, nonfastidious types which are ubiquitous in soil and air and commonly contaminate laboratory cultures of pathogens. Predominant isolates were species of Aspergillus, Penicillium, Pullularia, Rhodotorula, and various yeasts. No instances of fungal proliferation were observed; test data reflect the survival of environmental types indigenous to the SSS pretests.
The Apollo Lunar Surface Experiments Package (ALSEP) measures lunar physical and environmental characteristics and transmits the data to receiving stations on earth. The data are used to derive information on the composition and structure of the moon. The electrical power subsystem generates and conditions all the electrical power for operations of the ALSEP system. The power source for the ALSEP is the Systems for Nuclear Auxiliary Power (SNAP) Radioisotope Thermoelectric Generator (RTG). The generator produces electricity by thermoelectric conversion. The radioactive isotope plutonium 238 is used as the energy source for the SNAP-27. By April 1, 1970, the SNAP-27 RTG had produced more than 230 kWh of continuous and stable power for the ALSEP.-
The applications are reported of new remote sensing techniques for earth resources surveys and environmental monitoring. Applications discussed include: vegetation systems, environmental monitoring, and plant protection. Data processing systems are described.
The thrust of New Jersey's ERTS investigation is development of procedures for operational use of ERTS-1 data by the Department of Environmental Protection in the management of the State's coastal zone. Four major areas of concern were investigated: detection of land use changes in the coastal zone; monitoring of offshore waste disposal; siting of ocean outfalls; and allocation of funds for shore protection. ERTS imagery was not useful for shore protection purposes; it was of limited practical value in the evaluation of offshore waste disposal and ocean outfall siting. However, ERTS imagery shows great promise for operational detection of land use changes in the coastal zone. Some constraints for practical change detection have been identified.
Skylab showed that a multidisciplinary manned space station is practical and can be highly productive. Quality data was produced concerning earth resources (mineral resources, agriculture, climatology, etc.), solar astronomy (solar activity, magnetic fields, and solar winds), materials sciences (e.g., zero-gravity crystal growth), and astronomy and astrophysics (including observations of comet Kohoutek). Two types of astronaut maneuvering unit were tested. Medical data are applicable to adaptational environmental physiology.
Small-scale color-infrared aerial photography at 1:120,000 scale is used to carry out an operational survey of coal refuse sites. Site analyses and reclamation cost estimates are completed in less than 90 days by using remote sensing techniques. Analysis of photographs provided dependable and accurate data for the location and environmental assessment of nearly 200 coal refuse banks and slurry ponds. The inventory constitutes a comprehensive reference for establishing priorities for coal refuse site reclamation and for complementing future field surveys.
An investigation was conducted to establish the feasibility of utilizing remotely sensed data acquired from aircraft and satellite platforms to provide information concerning the distribution and abundance of oceanic gamefish. The data from the test area was jointly acquired by NASA, the Navy, the Air Force and NOAA/NMFS elements and private and professional fishermen in the northeastern Gulf of Mexico. The data collected has made it possible to identify fisheries significant environmental parameters for white marlin. Prediction models, based on catch data and surface truth information, were developed and demonstrated a potential for significantly reducing search by identifying areas that have a high probability of productivity. Three of the parameters utilized by the models, chlorophyll-a, sea surface temperature, and turbidity were inferred from aircraft sensor data and were tested. Effective use of Skylab data was inhibited by cloud cover and delayed delivery. Initial efforts toward establishing the feasibility of utilizing remotely sensed data to assess and monitor the distribution of oceanic gamefish has successfully identified fisheries significant oceanographic parameters and demonstrated the capability of remotely measuring most of the parameters.
The manufacturing and processing procedures which were used to fabricate a precision graphite/epoxy support structure for a spherical microwave reflecting surface are described. The structure was made fromm GY-70/930 ultra high modulus graphite prepreg, laminated to achieve an isotropic in plane thermal expansion of less than + or - 0.1 PPM/F. The structure was hand assembled to match the interface of the reflective surface, which was an array of 18 flexure supported, aluminum, spherically contoured tiles. Structural adhesives were used in the final assembly to bond the elements into their final configuration. A eutectic metal coating was applied to the composite surface to reduce dimensional instabilities arising from changes in the composite epoxy moisture content due to environmental effects. Basic materials properties data are reported and the results of a finite element structural analysis are referenced.
The system and program requirements for a space operations center as defined by systems analysis studies are presented as a guide for future study and systems definition. Topics covered include general requirements for safety, maintainability, and reliability, service and habitat modules, the health maintenance facility; logistics modules; the docking tunnel; and subsystem requirements (structures, electrical power, environmental control/life support; extravehicular activity; data management; communications and tracking; docking/berthing; flight control/propulsion; and crew support). Facilities for flight support, construction, satellite and mission servicing, and fluid storage are included as well as general purpose support equipment.
The Infrared Telescope (IRT) is designed to survey extended celestial sources of infrared radiation between 4 and 120 micrometers wavelength. It will provide data regarding Space Shuttle induced environmental contamination and the zodical light. And, it will provide experience in the management of large volumes of superfluid helium in the space environment.
The design factors being considered in the NASA space-station development program are summarized. The currently envisioned mission requirements are listed, and the system architecture is defined as a core station, mission-dedicated elements, and supporting equipment such as an orbit maneuvering vehicle. System design factors discussed include orbit selection, contamination control, autonomy, system safety, technology implementation, long life, reliability and maintainability, and cost; subsystem design factors include structural considerations, electrical power, environmental control and life support, data management, communications and tracking, onboard propulsion, habitability, and crew support. Configurational design is seen as driven by a number of factors, primarily the need to fit all components into the Shuttle payload bay for assembly in LEO by the Shuttle crew.
The ability of a 406-MHz satellite to detect and locate aeronautical, maritime, and terrestrial distress incidents is investigated. The geostationary satellite, uplink and downlink budgets, and processors utilized in the experiment are described. The experiment involves: (1) benchmark testing, (2) verification of spacecraft repeater performance, (3) system evaluation, and (4) environmental testing. The Canadian processor data reveal that the processor threshold is 28 dB Hz, the degradation at 27 dB Hz is significant, the median distress message throughput time is 6 minutes, and the microcomputer can handle 10 distress incidents per hour.