Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Activity detection”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,351 records · Page 75

Learning Behavior of Distribution System Discrete Control Devices for Cyber-Physical Security

Conventional cyber-security intrusion detection systems monitor network traffic for malicious activity and indications that an adversary has gained access to the system. The approach discussed here expands the idea of a traditional intrusion detection system within electrical power systems, specifically power distribution networks, by monitoring the physical behavior of the grid. This is achieved through the use of high-rate distribution Phasor Measurement Units (PMUs), alongside SCADA packets analysis, for the purpose of monitoring the behavior of discrete control devices. In this work we present a set of algorithms for passively learning the control logic of voltage regulators and switched capacitor banks. Upon detection of an abnormal operation, the operator is alerted and further action can be taken. Finally, the proposed learning algorithms are validated on both simulated data and on measured PMU data from a utility pilot deployment site.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Revalidation of recombinant aequorin as a light emission standard: Estimation of specific activity of Gaussia luciferase

Highlights: • Recombinant aequorin (reAequorin) was evaluated for use as a light emission standard. • reAequorin protein concentration was determined by amino acid composition analysis. • The presence of active reAequorin was confirmed by the absorption peak ratio at 280/460 nm. • The I{sub max} value of Gaussia luciferase was 7.5-fold higher than that of reAequorin. • reAequorin can generally be used as a light emission standard. To validate the use of recombinant aequorin (reAequorin) as a light emission standard, the protein concentrations of highly purified reAequorin were determined by amino acid composition analysis, and the presence of active reAequorin was confirmed by the ratio of absorbance peak at 460 nm to that at 280 nm. The high correlation of the luminescence intensity with the protein concentration showed that reAequorin could be used for a light emission standard to study the luminescence properties of luciferases and to evaluate the detection sensitivity of luminometers. The specific activity of Gaussia luciferase with I{sub max} was 7.5-fold higher than that of reAequorin and was calculated to be 3.8 × 10{sup 16} quanta/mg protein.

60 APPLIED LIFE SCIENCES↗

The Winter 2010 and 2011 FRONT/NIRSS In-Flight Icing Hazard Detection Project

The NASA Icing Remote Sensing System (NIRSS) deploys a vertically-pointing K-band radar, a lidar ceiliometer, and a profiling microwave radiometer to obtain measurements for diagnosing local inflight icing conditions. RAL is working with NASA GRC to develop algorithms and data ingest and display software for the system. NASA has an ongoing activity to develop remote sensing technologies for the detection and measurement of icing conditions aloft. As part of that effort NASA teamed with NCAR to develop software that fuses data from multiple instruments into a single detected icing condition product. The multiple instrument approach, which is the current emphasis of this activity, utilizes a K-band vertical staring radar, a microwave radiometer that detects twelve frequencies between 22 and 59 GHz, and a lidar ceilometer. The radar data determine cloud boundaries, the radiometer determines the sub-freezing temperature heights and total liquid water content, and the ceilometer refines the lower cloud boundary. Data is post-processed in C++ program with a Java-based web display of resultant supercooled LWC profile and aircraft hazard identification. In 2010, a multi-channel scanning radiometer, designed and built by Radiometrics, Inc. under a SBIR grant,,was added to the system to assess its utility in improving icing diagnoses.

Serke, David↗

c-Src activation through a TrkA and c-Src interaction is essential for cell proliferation and hematological malignancies

Highlights: •TrkA was mainly present in other types of leukemia including AML. •TrkA enhances the survival of leukemia by activation of PI3K/Akt pathway. •TrkA induced significant hematological malignancies by inducing PLK-1 and Twist-1. •TrkA acted as a key regulator of leukemogenesis and survival through c-Src activation. -- Abstract: Although the kinase receptor TrkA may play an important role in acute myeloid leukemia (AML), its involvement in other types of leukemia has not been reported. Furthermore, how it contributes to leukemogenesis is unknown. Here, we describe a molecular network that is important for TrkA function in leukemogenesis. We found that TrkA is frequently overexpressed in other types of leukemia such as acute lymphoblastic leukemia (ALL), chronic myelogenous leukemia (CML), and myelodysplastic syndrome (MDS) including AML. In addition, TrkA was overexpressed in patients with MDS or secondary AML evolving from MDS. TrkA induced significant hematological malignancies by inducing PLK-1 and Twist-1, and enhanced survival and proliferation of leukemia, which was correlated with activation of the phosphatidylinositol 3-kinase/Akt/mTOR pathway. Moreover, endogenous TrkA associated with c-Src complexes was detected in leukemia. Suppression of c-Src activation by TrkA resulted in markedly decreased expression of PLK-1 and Twist-1 via suppressed activation of Akt/mTOR cascades. These data suggest that TrkA plays a key role in leukemogenesis and reveal an unexpected physiological role for TrkA in the pathogenesis of leukemia. These data have important implications for understanding various hematological malignancies.

60 APPLIED LIFE SCIENCES↗

The NASA Icing Remote Sensing System

NASA and the U.S. Army Cold Regions Research and Engineering Laboratory (CRREL) have an on-going activity to develop remote sensing technologies for the detection and measurement of icing conditions aloft. A multiple instrument approach is the current emphasis of this activity. Utilizing radar, radiometry, and lidar, a region of supercooled liquid is identified. If the liquid water content (LWC) is sufficiently high, then the region of supercooled liquid cloud is flagged as being an aviation hazard. The instruments utilized for the current effort are an X-band vertical staring radar, a radiometer that measures twelve frequencies between 22 and 59 GHz, and a lidar ceilometer. The radar data determine cloud boundaries, the radiometer determines the sub-freezing temperature heights and total liquid water content, and the ceilometer refines the lower cloud boundary. Data are post-processed with a LabVIEW program with a resultant supercooled LWC profile and aircraft hazard identification. Remotely sensed measurements gathered during the 2003-2004 Alliance Icing Research Study (AIRS II) were compared to aircraft in-situ measurements. Although the comparison data set is quite small, the cases examined indicate that the remote sensing technique appears to be an acceptable approach.

Reehorst, Andrew L.↗

NASA Icing Remote Sensing System Comparisons From AIRS II

NASA has an on-going activity to develop remote sensing technologies for the detection and measurement of icing conditions aloft. A multiple instrument approach is the current emphasis of this activity. Utilizing radar, radiometry, and lidar, a region of supercooled liquid is identified. If the liquid water content (LWC) is sufficiently high, then the region of supercooled liquid cloud is flagged as being an aviation hazard. The instruments utilized for the current effort are an X-band vertical staring radar, a radiometer that measures twelve frequencies between 22 and 59 GHz, and a lidar ceilometer. The radar data determine cloud boundaries, the radiometer determines the sub-freezing temperature heights and total liquid water content, and the ceilometer refines the lower cloud boundary. Data is post-processed with a LabVIEW program with a resultant supercooled LWC profile and aircraft hazard identification. Individual remotely sensed measurements gathered during the 2003-2004 Alliance Icing Research Study (AIRS II) were compared to aircraft in-situ measurements. Comparisons between the remote sensing system s fused icing product and in-situ measurements from the research aircraft are reviewed here. While there are areas where improvement can be made, the cases examined indicate that the fused sensor remote sensing technique appears to be a valid approach.

Reehorst, Andrew L.↗

Antiorthostatic suspension stimulates profiles of macrophage activation in mice

The antiorthostatic suspension model simulates certain physiological effects of spaceflight. We have previously reported BDF1 mice suspended by the tail in the antiorthostatic orientation for 4 days express high levels of resistance to virulent Listeria monocytogenesinfection. In the present study, we examined whether the increased resistance to this organism correlates with profiles of macrophage activation, given the role of the macrophage in killing this pathogen in vivo. We infected BDF1 mice with a lethal dose of virulent L. monocytogenes on day 4 of antiorthostatic suspension and 24 h later constructed profiles of macrophage activation. Viable listeria could not be detected in mice suspended in the antiorthostatic orientation 24 h after infection. Flow cytometric analysis revealed the numbers of granulocytes and mononuclear phagocytes in the spleen of infected mice were not significantly altered as a result of antiorthostatic suspension. Splenocytes from antiorthostatically suspended infected mice produced increased titers of IL-1. Serum levels of neopterin, a nucleotide metabolite secreted by activated macrophages, were enhanced in mice infected during antiorthostatic suspension, but not in antiorthostatically suspended naive mice. Splenic macrophages from mice infected on day 4 of suspension produced enhanced levels of lysozyme. In contrast to the results from antiorthostatically suspended infected mice, macrophages from antiorthostatically suspended uninfected mice did not express enhanced bactericidal activities. The collective results indicate that antiorthostatic suspension can stimulate profiles of macrophage activation which correlate with increased resistance to infection by certain classes of pathogenic bacteria.

Non-NASA Center↗

Neutrino constraints: what large-scale structure and CMB data are telling us?

We discuss the reliability of neutrino mass constraints, either active or sterile, from the combination of different low redshift Universe probes with measurements of CMB anisotropies. In our analyses we consider WMAP 9-year or Planck Cosmic Microwave Background (CMB) data in combination with Baryonic Acoustic Oscillations (BAO) measurements from BOSS DR11, galaxy shear measurements from CFHTLenS, SDSS Ly α forest constraints and galaxy cluster mass function from Chandra observations. At odds with recent similar studies, to avoid model dependence of the constraints we perform a full likelihood analysis for all the datasets employed. As for the cluster data analysis we rely on to the most recent calibration of massive neutrino effects in the halo mass function and we explore the impact of the uncertainty in the mass bias and re-calibration of the halo mass function due to baryonic feedback processes on cosmological parameters. We find that none of the low redshift probes alone provide evidence for massive neutrino in combination with CMB measurements, while a larger than 2σ detection of non zero neutrino mass, either active or sterile, is achieved combining cluster or shear data with CMB and BAO measurements. Yet, the significance of the detection exceeds 3σ if we combine all four datasets. For a three active neutrino scenario, from the joint analysis of CMB, BAO, shear and cluster data including the uncertainty in the mass bias we obtain ∑ m{sub ν} =0.29{sup +0.18}{sub -0.21} eV and ∑ m{sub ν} =0.22{sup +0.17}{sub -0.18} eV 95%CL) using WMAP9 or Planck as CMB dataset, respectively. The preference for massive neutrino is even larger in the sterile neutrino scenario, for which we get m{sub s}{sup eff}=0.44{sup +0.28}{sub -0.26} eV and Δ N{sub eff}=0.78{sup +0.60}{sub -0.59} 95%CL) from the joint analysis of Planck, BAO, shear and cluster datasets. For this data combination the vanilla ΛCDM model is rejected at more than 3σ and a sterile neutrino mass as motivated by accelerator anomaly is within the 2σ errors. Conversely, the Ly α data favour vanishing neutrino masses and from the data combination Planck+BAO+Ly α we get the tight upper limits ∑ m{sub ν} <0.14 eV and m{sub s}{sup eff}<0.22 eV—Δ N{sub eff}<1.11 95%CL) for the active and sterile neutrino model, respectively. Finally, results from the full data combination reflect the tension between the σ{sub 8} constraints obtained from cluster and shear data and that inferred from Ly α forest measurements; in the active neutrino scenario for both CMB datasets employed, the full data combination yields only an upper limits on ∑ m{sub ν}, while assuming an extra sterile neutrino we still get preference for non-vanishing mass, m{sub s}{sup eff}=0.26{sup +0.22}{sub -0.24} eV, and dark contribution to the radiation content, Δ N{sub eff}=0.82±0.55.

79 ASTRONOMY AND ASTROPHYSICS↗

Influence of Short Central PEO Segment on Hydrolytic and Enzymatic Degradation of Triblock PCL Copolymers

Hydrolytic, enzymatic degradation and composting under controlled conditions of series of triblock PCL/PEO copolymers, PCEC, with central short PEO block (M{sub n} 400 g/mol) are presented and compared with homopolymer (PCL). The PCEC copolymers, synthesized via ring-opening polymerization of ε-caprolactone, were characterized by {sup 1}H NMR, quantitative {sup 13}C NMR, GPC, DSC and WAXS. The introduction of the PEO central segment (< 2 wt%) in PCL chains significantly affected thermal degradation and crystallization behavior, while the hydrophobicity was slightly reduced as confirmed by water absorption and moisture uptake experiments. Hydrolytic degradation studies in phosphate buffer after 8 weeks indicated a small weight loss, while FTIR analysis detected changes in crystallinity indexes and GPC measurements revealed bulk degradation. Enzymatic degradation tested by cell-free extracts containing Pseudomonas aeruginosa PAO1 confirmed high enzyme activity throughout the surface causing morphological changes detected by optical microscopy and AFM analysis. The changes in roughness of polymer films revealed surface erosion mechanism of enzymatic degradation. Copolymer with the highest content of PEO segment and the lowest molecular weight showed better degradation ability compared to PCL and other copolymers. Furthermore, composting of polymer films in a model compost system at 37 °C resulted in significant degradation of the all synthesized block copolymers.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

Inhibition of enterovirus 71 entry by transcription factor XBP1

Highlights: Black-Right-Pointing-Pointer IRE1 was activated but no XBP1 splicing was detected during enterovirus 71 infection. Black-Right-Pointing-Pointer XBP1 was subject to translational shutoff by enterovirus 71-induced eIF4G cleavage. Black-Right-Pointing-Pointer The uptake of UV-irradiated virus was decreased in XBP1-overexpressing cells. -- Abstract: Inositol-requiring enzyme 1 (IRE1) plays an important role in the endoplasmic reticulum (ER), or unfolded protein, stress response by activating its downstream transcription factor X-box-binding protein 1 (XBP1). We demonstrated previously that enterovirus 71 (EV71) upregulated XBP1 mRNA levels but did not activate spliced XBP1 (XBP1s) mRNA or its downstream target genes, EDEM and chaperones. In this study, we investigated further this regulatory mechanism and found that IRE1 was phosphorylated and activated after EV71 infection, whereas its downstream XBP1s protein level decreased. We also found that XBP1s was not cleaved directly by 2A{sup pro}, but that cleavage of eukaryotic translation initiation factor 4G by the EV71 2A{sup pro} protein may contribute to the decrease in XBP1s expression. Knockdown of XBP1 increased viral protein expression, and the synthesis of EV71 viral protein and the production of EV71 viral particles were inhibited in XBP1-overexpressing RD cells. When incubated with replication-deficient and UV-irradiated EV71, XBP1-overexpressing RD cells exhibited reduced viral RNA levels, suggesting that the inhibition of XBP1s by viral infection may underlie viral entry, which is required for viral replication. Our findings are the first indication of the ability of XBP1 to inhibit viral entry, possibly via its transcriptional activity in regulating molecules in the endocytic machinery.

60 APPLIED LIFE SCIENCES↗

An Expert System for Autonomous Spacecraft Control

The Autonomous Sciencecraft Experiment (ASE), part of the New Millennium Space Technology 6 Project, is flying onboard the Earth Orbiter 1 (EO-1) mission. The ASE software enables EO-1 to autonomously detect and respond to science events such as: volcanic activity, flooding, and water freeze/thaw. ASE uses classification algorithms to analyze imagery onboard to detect chang-e and science events. Detection of these events is then used to trigger follow-up imagery. Onboard mission planning software then develops a response plan that accounts for target visibility and operations constraints. This plan is then executed using a task execution system that can deal with run-time anomalies. In this paper we describe the autonomy flight software and how it enables a new paradigm of autonomous science and mission operations. We will also describe the current experiment status and future plans.

utonomy flight software↗

THE HUNT FOR EXOMOONS WITH KEPLER (HEK). V. A SURVEY OF 41 PLANETARY CANDIDATES FOR EXOMOONS

We present a survey of 41 Kepler Objects of Interest (KOIs) for exomoons using Bayesian photodynamics, more than tripling the number of KOIs surveyed with this technique. We find no compelling evidence for exomoons although 13 KOIs yield spurious detections driven by instrumental artifacts, stellar activity, and/or perturbations from unseen bodies. Regarding the latter, we find seven KOIs exhibiting >5 σ evidence of transit timing variations, including the “mega-Earth” Kepler-10c, likely indicating an additional planet in that system. We exploit the moderately large sample of 57 unique KOIs surveyed to date to infer several useful statistics. For example, although there is a diverse range in sensitivities, we find that we are sensitive to Pluto–Charon mass-ratio systems for ≃40% of KOIs studied and Earth–Moon mass-ratios for 1 in 8 cases. In terms of absolute mass, our limits probe down to 1.7 Ganymede masses, with a sensitivity to Earth-mass moons for 1 in 3 cases studied and to the smallest moons capable of sustaining an Earth-like atmosphere (0.3 M{sub ⨁}) for 1 in 4. Despite the lack of positive detections to date, we caution against drawing conclusions yet, since our most interesting objects remain under analysis. Finally, we point out that had we searched for the photometric transit signals of exomoons alone, rather than using photodynamics, we estimate that 1 in 4 KOIs would have erroneously been concluded to harbor exomoons due to residual time correlated noise in the Kepler data, posing a serious problem for alternative methods.

79 ASTRONOMY AND ASTROPHYSICS↗

Disentangling Centrality Bias and Final-State Effects in the Production of High-𝑝 𝑇 Neutral Pions Using Direct Photon in 𝑑+Au Collisions at $\sqrt{s_{NN}}$ = 200 GeV

Here, PHENIX presents a simultaneous measurement of the production of direct 𝛾 and 𝜋 0 in 𝑑 + Au collisions at $\sqrt{s_{NN}}$ = 200 GeV over a 𝑝 𝑇 range of 7.5 to 18 GeV/𝑐 for different event samples selected by event activity, i.e., charged-particle multiplicity detected at forward rapidity. Direct-photon yields are used to empirically estimate the contribution of hard-scattering processes in the different event samples. Using this estimate, the average nuclear-modification factor, 𝑅$^{𝜋^0}_{dAu,EXP}$, is 0.925 ± 0.023⁢(stat) ± 0.15⁢(scale), consistent with unity for minimum-bias (MB) 𝑑+Au collisions. For event classes with low and moderate event activity, 𝑅$^{𝜋^0}_{dAu,EXP}$ is consistent with the MB value within 5% uncertainty. This result confirms that the previously observed enhancement of high-𝑝 𝑇 𝜋 0 production found in small-system collisions with low event activity is a result of a bias in interpreting event activity within the Glauber framework. In contrast, for the top 5% of events with the highest event activity, 𝑅$^{𝜋^0}_{dAu,EXP}$ is suppressed by 20% relative to the MB value with a significance of 4.5⁢𝜎, which may be due to final-state effects. This suppression corresponds to a 𝑝 𝑇 shift of 𝛿⁢𝑝 𝑇 = 0.213 ± 0.055 Gev/𝑐 at 9 Gev/𝑐.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Microbial Monitoring from the Frontlines to Space: Department of Defense Small Business Innovation Research Technology Aboard the International Space Station

The RAZOR (trademark) EX, a quantitative Polymerase Chain Reaction (qPCR) instrument, is a portable, ruggedized unit that was designed for the Department of Defense (DoD) with its reagent chemistries traceable to a Small Business Innovation Research (SBIR) contract beginning in 2002. The PCR instrument's primary function post 9/11 was to enable frontline soldiers and first responders to detect biological threat agents and bioterrorism activities in remote locations to include field environments. With its success for DoD, the instrument has also been employed by other governmental agencies including Department of Homeland Security (DHS). The RAZOR (Trademark) EX underwent stringent testing by the vendor, as well as through the DoD, and was certified in 2005. In addition, the RAZOR (trademark) EX passed DHS security sponsored Stakeholder Panel on Agent Detection Assays (SPADA) rigorous evaluation in 2011. The identification and quantitation of microbial pathogens is necessary both on the ground as well as during spaceflight to maintain the health of astronauts and to prevent biofouling of equipment. Currently, culture-based monitoring technology has been adequate for short-term spaceflight missions but may not be robust enough to meet the requirements for long-duration missions. During a NASA-sponsored workshop in 2011, it was determined that the more traditional culture-based method should be replaced or supplemented with more robust technologies. NASA scientists began investigating innovative molecular technologies for future space exploration and as a result, PCR was recommended. Shortly after, NASA sponsored market research in 2012 to identify and review current, commercial, cutting edge PCR technologies for potential applicability to spaceflight operations. Scientists identified and extensively evaluated three candidate technologies with the potential to function in microgravity. After a thorough voice-of-the-customer trade study and extensive functional and safety evaluations, the RAZOR (trademark) EX PCR instrument(Bio-Fire Defense, Salt Lake City, UT) was selected as the most promising current technology for spaceflight monitoring applications.

Cherie M Oubre↗

Detection of X-ray emission from the young low-mass star Rossiter 137B

Rst 137B, a close M-dwarf companion to the active K-star HD 36705, has been detected in a High Resolution Image in the Einstein Observatory Archive. The X-ray surface fluxes (0.2-4 keV) from both stars are close to the empirical saturation level, F(x)/F(bol) of about 0.001, defined by rapid rotators and very young stars. This supports the earlier results of the youthfulness of the system. This young couple is an excellent subject for studies of dependence of early evolution on stellar mass. Rst 137B is one of the latest spectral types and thus lowest-mass premain-sequence stars yet detected as an X-ray source.

Vilhu, O.↗

Passive Noise Analysis for Advanced Tamper Indication. End of Year Report 2015

Idaho National Laboratory (INL) is part of a multi-lab project assessing front-end electronics for unattended measurement (FEUM) being developed for the International Atomic Energy Agency (IAEA) unattended systems. The FEUM development activity provides an opportunity to address tampering detection between FEUM and the detector, signal integrity from FEUM to the data acquisition systems, and data validity – long-standing challenges for the IAEA. This report summarizes the INL activities in Fiscal Year (FY) 2015 to characterize and test passive noise analysis as a potential tamper-indicating approach for implementation into FEUM or as a stand-alone method. The project’s primary objectives in FY-15 were to (1) determine detectable tamper scenarios using four pre-amplifier/detector systems, (2) perform tests of tampering scenarios with three common cable types used by the IAEA, (3) separate radio-frequency-induced events from inherent effect by means of an anechoic chamber, and (4) perform tests at an industrial facility. General conclusions were reached in several areas.

98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL P↗