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At least 1,333 records · Page 74

Signal Processing Variables for Optimization of Flaw Detection in Composites Using Ultrasonic Guided Wave Scanning

This study analyzes the effect of signal processing variables on the ability of the ultrasonic guided wave scan method at NASA Glenn Research Center to distinguish various flaw conditions in ceramic matrix composites samples. In the ultrasonic guided wave scan method, several time- and frequency-domain parameters are calculated from the ultrasonic guided wave signal at each scan location to form images. The parameters include power spectral density, centroid mean time, total energy (zeroth moment), centroid frequency, and ultrasonic decay rate. A number of signal processing variables are available to the user when calculating these parameters. These signal processing variables include 1) the time portion of the time-domain waveform processed, 2) integration type for the properties requiring integrations, 3) bounded versus unbounded integrations, 4) power spectral density window type, 5) and the number of time segments chosen if using the short-time fourier transform to calculate ultrasonic decay rate. Flaw conditions examined included delamination, cracking, and density variation.

Roth, Don J.↗

Characteristics of infrasound signals from North Korean underground nuclear explosions on 2016 January 6 and September 9

This study investigates infrasound signals from North Korean underground nuclear explosions on 2016 January 6 at 01:30:01 UTC (UNEJ16) and on 2016 September 9 at 00:30:01 UTC (UNES16). Infrasound observations from eight, seismo-acoustic arrays at distances of 300–600 km from the explosions and aligned approximately east–west across South Korea are used in the analysis. Progressive multichannel correlation was used followed by analyst review to identify the infrasound arrivals with estimates of azimuth, phase velocity, celerity, F-statistic, signal-to-noise ratio, correlation and signal duration. These observations are compared with model predictions of travel path, ray turning height and relative amplitudes, using Ground-to-Space atmospheric specifications at the time of each explosion. Stratospheric conditions at the time of the UNEJ16 are favourable to the Korean arrays when all arrays detected infrasound signals, while the few detections for UNES16 are consistent with unfavourable stratospheric winds and reduced amplitude thermospheric arrivals. Infrasound locations of the explosions were estimated using the Bayesian Infrasonic Source Location method (BISL), with wind-corrected backazimuth estimates based on the ray tracing calculations. Adding wind corrections to BISL resulted in infrasound locations up to 50 per cent closer to the seismic epicentre over uncorrected locations. The UNES16 infrasound location estimate using atmospheric corrections had larger differences (~67 km difference from seismic location) than the UNEJ16 location (~11 km difference from seismic location) as a result of the reduced number of observations and poor azimuth coverage for UNES16. Based on stratospheric phases recorded at six arrays and the empirical yield-scaling relations, wind corrected amplitudes from UNEJ16 lead to equivalent infrasound source energy estimates between 0.9 and 16.1 tons of TNT detonated in the atmosphere with an average of 6.4 tons and a standard deviation of 4.6 tons.

58 GEOSCIENCES↗

Data storage, image tube type

Method and apparatus for the storage of digital or analog electrical signals are provided by a memory storage system employing a conventional vidicon tube. At the beginning of an operating cycle, the vidicon is conditioned to accept electrical data input by exposing its photosensitive target to a short, high intensity light flash. A first electron beam scan of the photosensitive surface then sets up a charge pattern on the photosensitive target. A second electron beam scan of the photosensitive surface by an unmodulated electron beam then develops an output signal across an output resistor by using capacitive currents. The conditioning and scanning steps are operated repetitively at high speed using conventional television camera scan, sync, and power supply circuitry to provide a low cost data storage system.

Lipoma, P. C.↗

Comparison of the Wavelet and Gabor Transforms in the Spectral Analysis of Nonstationary Signals

Two approaches to the analysis of nonstationary random processes (short-time Fourier transform and continuous wavelet transform) are compared. The comparison is based on the study of several model signals with known time–frequency characteristics. The application of the approaches is also analyzed in the study of spectral dynamics of fluorescence of cold atomic clouds excited by pulsed radiation. It is shown that the two approaches make it possible to reveal the main specific features of the signals under study. However, the continuous wavelet transform has several advantages, since the optimal conditions for the analysis using the short-time Fourier transform are reached if additional calculations aimed at determination of the optimal width of the window are performed.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Solid State Power Substation DC Node Optimization and Controller Hardware-In-The-Loop Demonstration

A solid state power substation (SSPS) node is a microgrid that integrates distributed energy resources and loads and injects/absorbs power to/from the SSPS distribution network. It is an essential building block of a futuristic distribution grid network. This paper presents the development and demonstration of optimization use cases of a SSPS DC node. By adopting multi-layer hierarchical control architecture and developing automatic device identification and dynamic optimization formulation algorithms, the SSPS DC node can perform plug-and-play resource integration and seamless transition of the optimized node operation under on and off grid condition without sophisticated algorithms, control mode changes, and user interactions. Four optimization use cases including economic dispatches with price signal changes, a sudden PV power drop, and a single directional meter and its associated costs with sending power back to the grid, and resiliency under a grid inverter trip condition were demonstrated through the real-time controller hardware-in-the-loop simulation.

Kim, Namwon↗

A case study of microphysical structures and hydrometeor phase in convection using radar Doppler spectra at Darwin, Australia

To understand the microphysical processes that impact diabatic heating and cloud lifetimes in convection, we need to characterize the spatial distribution of supercooled liquid water. To address this observational challenge, vertically pointing active sensors at the Darwin Atmospheric Radiation Measurement (ARM) site are used to classify cloud phase within a deep convective cloud in a shallow to deep convection transitional case. The cloud cannot be fully observed by a lidar due to signal attenuation. Thus we develop an objective method for identifying hydrometeor classes, including mixed-phase conditions, using k-means clustering on parameters that describe the shape of the Doppler spectra from vertically pointing Ka band cloud radar. This approach shows that multiple, overlapping mixed-phase layers exist within the cloud, rather than a single region of supercooled liquid, indicating complexity to how ice growth and diabatic heating occurs in the vertical structure of the cloud.

54 ENVIRONMENTAL SCIENCES↗

Transcription of blunt snout bream (Megalobrama amblycephala) HIF3α and its localization in the nucleus under both normoxic and hypoxic conditions

Highlights: • HIF3α cDNA was characterized the in hypoxia-sensitive blunt snout bream. • HIF3α mRNA was stably expressed during embryogenesis and different adult tissues. • HIF3α mRNA was upregulated in the liver, brain, and kidney under hypoxia. • HIF3α was distributed in the nucleus under normoxic and hypoxic conditions. • Hypoxia simulation caused a significant increase in HIF3α protein levels. • There was a nuclear localization signal (NLS) sequence at the C-terminus of HIF3α. Although hypoxia-inducible factor (HIF) 1α and 2α function as master regulators of the transcriptional response to hypoxia, the function of HIF3α and its responses to hypoxic stress remain unclear in teleost fish. Here, we characterized the HIF3α cDNA in hypoxia-sensitive blunt snout bream (Megalobrama amblycephala), with 3059 bp length, consisting of an open reading frame (ORF) encoding 643 amino acid residues. Blunt snout bream HIF3α mRNA was stably expressed during stages of embryonic development and in adult tissues. After a 4 h hypoxia stress, HIF3α mRNA of the juvenile fish was significantly upregulated in the liver, brain, and kidney, and restored to the pretreatment levels after a 24 h recovery. When tagged with enhanced green fluorescent protein (EGFP) and transfected into cultured HeLa cells, blunt snout bream HIF3α was mainly distributed in the nucleus under normoxia. Treatment of the cells with CoCl{sub 2} to mimic hypoxic conditions showed that there was no effect about the nuclear localization of HIF3α but a statistically significant increase in HIF3α protein levels. A nuclear localization signal (NLS) sequence at the C-terminus of HIF3α may exert positive effects in the process of nuclear localization. These results suggest that blunt snout bream HIF3α could be involved in different physiological functions under normoxia and hypoxia conditions.

60 APPLIED LIFE SCIENCES↗

Helium Release During Fracture and Granular Fragmentation of Rocks

Geogenic Helium-4 ( 4 He) in-situ increases locally in regions of large deformation generated naturally or anthropogenically. This gas release by deformation is a potential geochemical precursor signal for subsurface deformation. To evaluate the applicability of 4 He degassing for correlating deformation in different lithologies, we conducted high force crush tests, up to 97,800 N axial load, to assess the total 4 He released during fragmentation of the rocks. We observed that the highest 4 He released occurred in the sedimentary rocks and that release correlated strongly with lithologic age and U/Th content. Microstructural changes of the pre- and post-test rocks indicate that the degree of grain size reduction relates directly to the total 4 He released during crushing. The range of in-place 4 He was calculated based XRF measurements of uranium and thorium in each lithology, with the results indicating that the majority of the trapped 4 He was not released. However, the 4 He released by deformation depended upon how the each rock deformed during deformation and the degree of grain size reduction. We postulate that 4 He precursor signals can be used to understand subsurface deformation only if geomechanical and geochemical conditions for 4 He enrichment in a lithology are met.

Deformation signals↗

SU-F-T-318: Sensitivity and Stability of OSLDs with Filled Deep Electron/hole Traps Under Pre-Irradiation and Bleaching Conditions

Purpose: This work evaluated the characteristics of optically stimulated luminescence dosimeters (OSLDs) with fully filled deep electron/hole traps (OSLDfull) with the bleaching conditions according to the accumulated dose. Methods: The OSLDs were first pre-irradiated with a Co-60 gamma ray at more than 5 kGy, so as to fill the deep electron and hole traps. Using a 6-MV beam, the OSLDfull characteristics were investigated in terms of the full bleaching, fading, dose linearity, and dose sensitivity obtained in response to the accumulated dose values. To facilitate a comparison of the dose sensitivity, OSLDs with un-filled deep electron/hole traps (OSLDempty) were investigated in the same manner. A long-pass filter was used to exclude bleaching-source wavelengths of less than 520 nm. Various bleaching time and wavelength combinations were used in order to determine the optimal bleaching conditions for the OSLD full. Results: The fading for the OSLDfull exhibited stable signals after 8 min, for both 1- and 10-Gy. For 4-h bleaching time and an unfiltered bleaching device, the supralinear index values for the OSLDfull were 1.003, 1.002, 0.999, and 1.001 for doses of 2, 4, 7, and 10 Gy, respectively. For a 65-Gy accumulated dose with a 5-Gy fraction, no variation in dose sensitivity was obtained for the OSLDfull, within a standard deviation of 0.85%, whereas the OSLDempty dose sensitivity decreased by approximately 2.3% per 10 Gy. The filtered bleaching device yielded a highly stable sensitivity for OSLDfull, independent of bleaching time and within a standard deviation of 0.71%, whereas the OSLDempty dose sensitivity decreased by approximately 4.2% per 10 Gy for an accumulated dose of 25 Gy with a 5-Gy fraction. Conclusion: Under the bleaching conditions determined in this study, clinical dosimetry with OSLDfull is highly stable, having an accuracy of 1% with no change in dose sensitivity or linearity at clinical doses. This work was supported by a National Research Foundation of Korea (NRF) grant, funded by the Korea government (MISP) (No. 2014M2B2A4031164), and by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C3459).

60 APPLIED LIFE SCIENCES↗

Optically activated switch/modulator using a photoconductor and two channel waveguides

An optically activated switch was fabricated on LiNbO3 substrate by deposition of photoconductor-metal electrode structure on two channel waveguides obtained by the outdiffusion process. One waveguide was overcoated with a CdS film, and served as a light detector which converted the optical signal in that waveguide into an electric signal. Utilizing the electrooptic effect, this electric signal modulated the light propagating in the second waveguide by changing the refractive index, and the cutoff condition, of the waveguiding channel between two metal electrodes. The waveguides in the optically controlled switch can be either single or multimode. The length of the device can be short in comparison with other switching schemes. When binary optical signals are applied to the two waveguides, the switch can perform logic AND, NEGATION, OR and pseudo-amplification operations.

Goldberg, L.↗

Off-of-equilibrium effects on Kurtosis Along Strangeness-Neutral Trajectories

The Beam Energy Scan program at the Relativistic Heavy Ion Collider (RHIC) is searching for the QCD critical point. The main signal for the critical point is the kurtosis of the distribution of proton yields obtained on an event by event basis where one expects a peak at the critical point. However, its exact behavior is still an open question due to out-of-equilibrium effects and uncertainty in the equation of state. Here we use a simplistic hydrodynamic model that enforces strangeness-neutrality, selecting trajectories that pass close to the critical point. We vary the initial conditions to estimate the effect of out-of-equilibrium hydrodynamics on the kurtosis signal.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

A Scanning Optical Quantum Magnetometer Based on the Hole Burning Phenomenon

A scanning optical quantum magnetometer with submicron spatial resolution is proposed that is based on the phenomenon of hole burning in the signal of optically detected magnetic resonance (ODMR) on atomic-sized color centers in silicon carbide crystals under conditions of spin level saturation by means of additional high-frequency resonance pumping. An increase in the sensitivity is achieved due to narrowing of the ODMR line and increasing slope of the dependence of signal frequency on the magnetic field.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Rotor blade imbalance fault detection for variable-speed marine current turbines via generator power signal analysis

Marine hydrokinetic (MHK) turbines extract renewable energy from oceanic environments. However, due to the harsh conditions that these turbines operate in, system performance naturally degrades over time. Thus, ensuring efficient condition-based maintenance is imperative towards guaranteeing reliable operation and reduced costs for marine hydrokinetic power. This work proposes a novel framework aimed at identifying and classifying the severity of rotor blade pitch imbalance faults experienced by marine current turbines (MCTs). In the framework, a Continuous Morlet Wavelet Transform (CMWT) is first utilized to acquire the wavelet coefficients encompassed within the 1P frequency range of the turbine's rotor shaft. From these coefficients, several statistical indices are tabulated into a six-dimensional feature space. Next, Principle Component Analysis (PCA) is employed on the resulting feature space for dimensionality reduction, and then the application of a K-Nearest Neighbor (KNN) machine learning algorithm is utilized for fault detection and severity classification. The effectiveness of the proposed framework is validated using a high-fidelity MCT numerical simulation platform, where results demonstrate that the presence of a pitch imbalance fault can be accurately detected 100% of the time and correctly classified based upon severity more than 97% of the time.

42 ENGINEERING↗

An algebraic method for constructing stable and consistent autoregressive filters

In this paper, we introduce an algebraic method to construct stable and consistent univariate autoregressive (AR) models of low order for filtering and predicting nonlinear turbulent signals with memory depth. By stable, we refer to the classical stability condition for the AR model. By consistent, we refer to the classical consistency constraints of Adams–Bashforth methods of order-two. One attractive feature of this algebraic method is that the model parameters can be obtained without directly knowing any training data set as opposed to many standard, regression-based parameterization methods. It takes only long-time average statistics as inputs. The proposed method provides a discretization time step interval which guarantees the existence of stable and consistent AR model and simultaneously produces the parameters for the AR models. In our numerical examples with two chaotic time series with different characteristics of decaying time scales, we find that the proposed AR models produce significantly more accurate short-term predictive skill and comparable filtering skill relative to the linear regression-based AR models. These encouraging results are robust across wide ranges of discretization times, observation times, and observation noise variances. Finally, we also find that the proposed model produces an improved short-time prediction relative to the linear regression-based AR-models in forecasting a data set that characterizes the variability of the Madden–Julian Oscillation, a dominant tropical atmospheric wave pattern.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

HIV-1 tat protein recruits CIS to the cytoplasmic tail of CD127 to induce receptor ubiquitination and proteasomal degradation

HIV-1 Tat protein down regulates expression of the IL-7 receptor alpha-chain (CD127) from the surface of CD8 T cells resulting in impaired T cell proliferation and cytolytic capacity. We have previously shown that soluble Tat protein is taken up by CD8 T cells and interacts with the cytoplasmic tail of CD127 to induce receptor degradation. The N-terminal domain of Tat interacts with CD127 while the basic domain directs CD127 to the proteasome. We have also shown that upon IL-7 binding to its receptor, CD127 is phosphorylated resulting in CIS-mediated proteasomal degradation. Here, we show that Tat mimics this process by recruiting CIS to CD127 in the absence of IL-7 and receptor phosphorylation, leading to CD127 ubiquitination and degradation. Tat therefore acts as an adapter to induce cellular responses under conditions where they may not otherwise occur. Thusly, Tat reduces IL-7 signaling and impairs CD8 T cell survival and function. -- Highlights: •Soluble HIV-1 Tat decreases CD127 expression on CD8 T cells, causing dysfunction. •Tat induces CD127 ubiquitination without activating IL-7 signaling. •Tat binds CD127 and recruits the E3 ubiquitin ligase CIS via its basic domain. •Tat hijacks a normal cellular mechanism to degrade CD127 without IL-7 signaling.

60 APPLIED LIFE SCIENCES↗

Entire expressed peripheral blood transcriptome in pediatric severe malarial anemia

Abstract This study on severe malarial anemia (SMA: Hb < 6.0 g/dL), a leading global cause of childhood morbidity and mortality, compares the entire expressed whole blood host transcriptome between Kenyan children (3-48 mos.) with non-SMA (Hb ≥ 6.0 g/dL, n = 39) and SMA ( n = 18). Differential expression analyses reveal 1403 up-regulated and 279 down-regulated transcripts in SMA, signifying impairments in host inflammasome activation, cell death, and innate immune and cellular stress responses. Immune cell profiling shows decreased memory responses, antigen presentation, and immediate pathogen clearance, suggesting an immature/improperly regulated immune response in SMA. Module repertoire analysis of blood-specific gene signatures identifies up-regulation of erythroid genes, enhanced neutrophil activation, and impaired inflammatory responses in SMA. Enrichment analyses converge on disruptions in cellular homeostasis and regulatory pathways for the ubiquitin-proteasome system, autophagy, and heme metabolism. Pathway analyses highlight activation in response to hypoxic conditions [Hypoxia Inducible Factor (HIF)−1 target and Reactive Oxygen Species (ROS) signaling] as a central theme in SMA. These signaling pathways are also top-ranking in protein abundance measures and a Ugandan SMA cohort with available transcriptomic data. Targeted RNA-Seq validation shows strong concordance with our entire expressed transcriptome data. These findings identify key molecular themes in SMA pathogenesis, offering potential targets for new malaria therapies.

60 APPLIED LIFE SCIENCES↗

Pressure-induced excimer formation and fluorescence enhancement of an anthracene derivative

Excimer materials have been widely studied and have generated significant interest for their applications in many optoelectronic devices. However, a thorough investigation of the entire process involved in excimer formation, enhancement, and annihilation in solid materials is still lacking. Here we designed a crystal based on an anthracene derivative with dissociative, molecular-ordered dimer assembly, and studied the formation and evolution of an anthracene excimer as a function of pressure. During the initial stage of pressurization, the fluorescence intensity arising from anthracene monomers gradually decreases. Therefore, with continued compression, the two anthracene units become increasingly closer allowing strong intermolecular π–π interactions to develop that lead to excimer formation at 3.5 GPa, accompanied with a phase transition. The fluorescence intensity then keeps increasing with pressure and reaches its maximum at 5.6 GPa due to the strengthening of the excimer and the increased structural defects. Meanwhile the fluorescence color shows a continuous redshift, which initially results from conformation planarization and then excimer evolution. After releasing pressure back to ambient conditions, the structural changes in the sample are reversible, while the fluorescence signal preserves some high-pressure features due to the partial retention of the π–π interactions between the anthracene dimers. This study reveals the evolution of an excimer and its intrinsic photophysical properties, and provides guidance for future research on pressure-sensitive fluorescent devices.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

High-frequency combination coding-based steady-state visual evoked potential for brain computer interface

This study presents a new steady-state visual evoked potential (SSVEP) paradigm for brain computer interface (BCI) systems. The goal of this study is to increase the number of targets using fewer stimulation high frequencies, with diminishing subject’s fatigue and reducing the risk of photosensitive epileptic seizures. The new paradigm is High-Frequency Combination Coding-Based High-Frequency Steady-State Visual Evoked Potential (HFCC-SSVEP).Firstly, we studied SSVEP high frequency(beyond 25 Hz)response of SSVEP, whose paradigm is presented on the LED. The SNR (Signal to Noise Ratio) of high frequency(beyond 40 Hz) response is very low, which is been unable to be distinguished through the traditional analysis method; Secondly we investigated the HFCC-SSVEP response (beyond 25 Hz) for 3 frequencies (25Hz, 33.33Hz, and 40Hz), HFCC-SSVEP produces n{sup n} with n high stimulation frequencies through Frequence Combination Code. Further, Animproved Hilbert-huang transform (IHHT)-based variable frequency EEG feature extraction method and a local spectrum extreme target identification algorithmare adopted to extract time-frequency feature of the proposed HFCC-SSVEP response.Linear predictions and fixed sifting (iterating) 10 time is used to overcome the shortage of end effect and stopping criterion,generalized zero-crossing (GZC) is used to compute the instantaneous frequency of the proposed SSVEP respondent signals, the improved HHT-based feature extraction method for the proposed SSVEP paradigm in this study increases recognition efficiency, so as to improve ITR and to increase the stability of the BCI system. what is more, SSVEPs evoked by high-frequency stimuli (beyond 25Hz) minimally diminish subject’s fatigue and prevent safety hazards linked to photo-induced epileptic seizures, So as to ensure the system efficiency and undamaging.This study tests three subjects in order to verify the feasibility of the proposed method.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗