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At least 1,333 records · Page 74

Effects of Altitude on Turbojet Engine Performance

Component and over-all performance characteristics of several turbojet engines investigated in the altitude facilities of the NACA Lewis Laboratory during the last several years are summarized to indicate the effects of altitude on turbojet engine performance. Data presented show that failure of turbojet engine performance to generalize for all altitudes can be traced to reductions in compressor efficiency, corrected air flow, and combustion efficiency at altitude. In addition, it is shown that although engines of different design may have equal thrusts at sea level, the thrusts at altitude may vary widely because of differences in compressor performance characteristics from one engine to another.

COMPRESSORS - EFFICIENCY↗

High Speed Data Acquisition for Real Time Feedback in a Light Duty Engine Combustion-Mode Switching Application

The paper describes the integration of a high-speed data acquisition and diagnostics controller used in an advanced engine platform. The controller enables ultra-low emissions and new benchmarks of engine efficiency while running a Gasoline Compression Ignition (GCI) cycle on a 2.2L, 4-cylinder engine. The system enables real-time combustion feedback and vibration analysis in engines. The paper focuses on: (1) the development of an interpolative sampling algorithm for transposition of time acquired data to the crank angle domain using a production crank sensor (60-2 tooth wheel); (2) the control unit, high-speed data acquisition, communication rates between the dedicated data acquisition and base controller to ensure cycle-to-cycle feedback; and, (3) validation exercises using cylinder pressure measurements. Here, the study shows how the algorithm resolves cylinder pressure information over an engine cycle, validating its robustness across acquisition rates of 50 and 200 kHz, with crank angle resolutions of 0.5° and 0.1°. Results show that the 50 kHz, 0.5° hardware yield combustion phasing and torque estimates within 0.1° and 1% torque of those attained with the 200 kHz, 0.1° hardware. Finally, study shows the dedicated controller and communication speeds allow for next-cycle fuel injection correction for speeds above 5000 rpm.

42 ENGINEERING↗

Multiple injection for improving knock, gaseous and particulate matter emissions in direct injection SI engines

Advances in fuel injector technology have enabled research and development on a large variety of direct injection spark ignition (DISI) engine fueling strategies targeted to improve engine performance and reduce engine-out emissions. This study explores the effect of multiple injections on knock, engine efficiency and stability, and particulate number and gaseous emissions on a single-cylinder DISI research engine. Work to date on multiple injections in the literature is reviewed, and then two aspects of multiple injection strategies are experimentally investigated: the number of injections (up to five times in a cycle); and the timing of the injections (classified relative to the timing of intake valve opening and closing). A boosted, single-cylinder research engine equipped with a state-of-the-art piezoelectric hollow cone spray direct injector and research-grade E10 gasoline was used for the study. The results show multiple injections maintain torque and combustion stability compared with single injection and slightly increase the knock limits and indicated thermal efficiencies (maximum 0.7% absolute, 2.8% relative compared with single-injection baseline) due to improved heat release phasing, especially with an additional late injection during the intake valve closed (compression stroke) period. The gaseous pollutant emissions including nitrogen oxides and unburned hydrocarbons were reduced by 25% with multiple injections, particularly with injection during the compression stroke. In contrast, carbon monoxide emissions increased with multiple injections for all conditions. Increasing the number of injection events significantly reduced particulate number emissions (half with each additional injection), and the decrease in particulate number was not sensitive to the injection timing.

42 ENGINEERING↗

Investigation of fuel film formation and soot emissions in a GDI engine during cold-start with Split-injection strategies

This study investigates the impact of fuel-film formation on engine-out soot emissions in a gasoline direct injection (GDI) engine under cold-start conditions. Split-injection strategies were applied by varying the number of injections, injection duration, and total fuel quantity to affect wall wetting and control the average in-cylinder equivalence ratio. A combined experimental and numerical approach was employed to analyze fuel-film deposition, combustion efficiency, and engine-out soot and unburnt hydrocarbons (UHC) emissions. In particular, fuel film distribution estimated by means of non-reacting, 3-D, computational fluid dynamics (CFD) simulations, together with experimentally measured soot data, were used to investigate fuel film formation and its role in soot generation. In the experiments, a skip-fired engine control strategy was applied to mimic the transient nature of engine cold-start operation. The results indicate that, under the same total number of injections, increasing the average in-cylinder equivalence ratio through longer injection durations improves combustion stability, as indicated by the decrease of the coefficient of variation of IMEP n (Net Indicated Mean Effective Pressure) from 6.0 % to 0.6 %. However, this strategy leads to higher soot emissions, which increased by nearly an order of magnitude, primarily due to enhanced wall-film formation. In contrast, increasing the number of injections while maintaining a constant equivalence ratio significantly impacts fuel-film deposition and, consequently, soot emissions, with a fivefold reduction of the measured engine-out soot, decreasing from 3.5 mg to 0.7 mg. A soot-film correlation was developed and achieved a high coefficient of determination (r 2 = 0.95) and was further extended to account for spark timing effects. These findings confirm the effectiveness of split-injection for avoiding wall film formation and soot emissions, and the critical role of fuel film in soot generation, supporting the hypothesis that pool fires play a crucial role in contributing to soot formation under these cold-start conditions. In conclusion, the study also indicates the value of a predictive soot-film correlation for developing cold-start emission control strategies.

Engine Cold-start↗

Structure-based protein engineering of bacterial β-xylosidase to increase the production yield of xylobiose from xylose

Highlights: • The crystal structures of β-xylosidase from Bacillus pumilus in complex with xylobiose were determined. • β-xylosidase was engineered to increase the yield of xylobiose based on the determined structures. • The protein engineering strategy used in this study could be applied within the same family of enzymes. Xylobiose consists of two molecules of xylose and has been highly recognized as a food supplement because it possesses high prebiotic functions. β-xylosidase exhibits enzymatic activity to hydrolyze xylobiose, and the enzyme can also catalyze the reverse reaction in the presence of high concentrations of xylose. Previously, β-xylosidase from Bacillus pumilus IPO (BpXynB), belonging to GH family 43, was employed to produce xylobiose from xylose. To improve the enzymatic efficiency, this study determined the high-resolution structure of BpXynB in a complex with xylobiose and engineered BpXynB based on the structures. The structure of BpXynB deciphered the residues involved in the recognition of the xylobiose. A site-directed mutation at the residue for xylobiose recognition increased the yield of xylobiose by 20% compared to a similar activity of the wild type enzyme. The complex structure of the mutant enzyme and xylobiose provided the structural basis for a higher yield of the engineered protein. This engineered enzyme would enable a higher economic production of xylobiose, and a similar engineering strategy could be applied within the same family of enzymes.

60 APPLIED LIFE SCIENCES↗

Climate change deniers, hippies, and preppers. Oh my!: Unpacking engineering student perceptions of renewable energy technology adoption in the United States

Engineers play a key role in the formation, operation, and maintenance of large socio-technical systems. Enmeshed in these practices of engineering are shared perceptions and visions of how society should be ordered, and what role of engineers should play. These value systems and how they shape the perception of who and where alternative energy belongs will shape who does or does not have the opportunity to participate in these alternative visions of energy. Drawing on engineering studies and dialectology research this study examines how a group of engineering students engaged in learning about renewable energy systems perceive the kinds of people in the United States who would or would not adopt alternative energy. Rather than see adoption/non-adoption as a purely “technical” matter, students brought their own perceptions of regional, social, and political differences to characterize the potential acceptability of each technology. Certain regions of the United States, despite their technical potential based on government and industry sources, are cast as too culturally backward and resistant to change to embrace new energy sources. In conclusion, these insights elucidate the importance of 'engineers' value systems as they continue to play a significant role in shaping the development of alternative energy technologies and markets.

Adoption↗

What fuel properties enable higher thermal efficiency in spark-ignited engines?

The Co-Optimization of Fuels and Engines (Co-Optima) initiative from the US Department of Energy aims to co-develop fuels and engines in an effort to maximize energy efficiency and the utilization of renewable fuels. Many of these renewable fuel options have fuel chemistries that are different from those of petroleum-derived fuels. Because practical market fuels need to meet specific fuel-property requirements, a chemistry-agnostic approach to assessing the potential benefits of candidate fuels was developed using the Central Fuel Property Hypothesis (CFPH). The CFPH states that fuel properties are predictive of the performance of the fuel, regardless of the fuel's chemical composition. In order to use this hypothesis to assess the potential of fuel candidates to increase efficiency in spark-ignition (SI) engines, the individual contributions towards efficiency potential in an optimized engine must be quantified in a way that allows the individual fuel properties to be traded off for one another. This review article begins by providing an overview of the historical linkages between fuel properties and engine efficiency, including the two dominant pathways currently being used by vehicle manufacturers to reduce fuel consumption. Then, a thermodynamic-based assessment to quantify how six individual fuel properties can affect efficiency in SI engines is performed: research octane number, octane sensitivity, latent heat of vaporization, laminar flame speed, particulate matter index, and catalyst light-off temperature. The relative effects of each of these fuel properties is combined into a unified merit function that is capable of assessing the fuel property-based efficiency potential of fuels with conventional and unconventional compositions.

42 ENGINEERING↗

Engineering the S-Layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials

Materials synthesized by organisms, such as bones and wood, combine the ability to self-repair with remarkable mechanical properties. This multifunctionality arises from the presence of living cells within the material and hierarchical assembly of different components across nanometer to micron scales. While creating engineered analogs of these natural materials is of growing interest, our ability to hierarchically order materials using living cells largely relies on engineered 1D protein filaments. Here, we lay the foundations for bottom-up assembly of engineered living material composites in 2D along the cell body using a synthetic biology approach. We engineer the paracrystalline surface-layer (S-layer) of Caulobacter crescentus to display SpyTag peptides that form irreversible isopeptide bonds to SpyCatcher-modified proteins, nanocrystals, and biopolymers on the extracellular surface. Using flow cytometry and confocal microscopy, we show that attachment of these materials to the cell surface is uniform, specific, and covalent, and its density can be controlled based on the location of the insertion within the S-layer protein, RsaA. Furthermore, we leverage the irreversible nature of this attachment to demonstrate via SDS-PAGE that the engineered S-layer can display a high density of materials, reaching 1 attachment site per 288 nm 2 . Lastly, we show that ligation of quantum dots to the cell surface does not impair cell viability and this composite material remains intact over a period of two weeks. Taken together, this work provides a platform for self-organization of soft and hard nanomaterials on a cell surface with precise control over 2D density, composition, and stability of the resulting composite, and is a key step towards building hierarchically-ordered engineered living materials with emergent properties.

59 BASIC BIOLOGICAL SCIENCES↗

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

Abstract CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts. As opposed to Cas9, the Cas12a endonucleases are characterized by a lack of trans-activating crRNA (tracrRNA), which reduces the complexity of the editing system and simultaneously makes CRISPR RNA (crRNA) engineering a promising approach toward further improving and modulating editing activity of the CRISPR-Cas12a systems. Here, we design and validate sixteen types of structurally engineered Cas12a crRNAs targeting various immunologically relevant loci in-vitro and in-cellulo . We show that all our structural modifications in the loop region, ranging from engineered breaks (STAR-crRNAs) to large gaps (Gap-crRNAs), as well as nucleotide substitutions, enable gene-cutting in the presence of various Cas12a nucleases. Moreover, we observe similar insertion rates of short HDR templates using the engineered crRNAs compared to the wild-type crRNAs, further demonstrating that the introduced modifications in the loop region led to comparable genome editing efficiencies. In conclusion, we show that Cas12a nucleases can broadly utilize structurally engineered crRNAs with breaks or gaps in the otherwise highly-conserved loop region, which could further facilitate a wide range of genome editing applications.

59 BASIC BIOLOGICAL SCIENCES↗

Pulsed laser heating of diesel engine and turbojet combustor soot: Changes in nanostructure and implications

Here, carbonaceous particulate produced by a diesel engine and turbojet engine combustor are analyzed by transmission electron microscopy (TEM) for differences in nanostructure before and after pulsed laser annealing. Soot is examined between low/high diesel engine torque and low/high turbojet engine thrust. Small differences in nascent nanostructure are magnified by the action of high-temperature annealing induced by pulsed laser heating. Lamellae length distributions show occurrence of graphitization while tortuosity analyses reveal lamellae straightening. Differences in internal particle structure (hollow shells versus internal graphitic ribbons) are interpreted as due to higher internal sp3 and O-atom content under the higher power conditions with hypothesized greater turbulence and resulting partial premixing. TEM in concert with fringe analyses reveal that a similar degree of annealing occurs in the primary particles in soot from both diesel engine and turbojet engine combustors—despite the aggregate and primary size differences between these sources. Implications of these results for source identification of the combustion particulate and for laser-induced incandescence (LII) measurements of concentration are discussed with inter-instrument comparison of soot mass from both diesel and turbojet soot sources.

42 ENGINEERING↗

Engine Combustion System Optimization Using Computational Fluid Dynamics and Machine Learning: A Methodological Approach

Gasoline compression ignition (GCI) engines are considered an attractive alternative to traditional spark-ignition and diesel engines. Here, a Machine Learning-Grid Gradient Ascent (ML-GGA) approach was developed to optimize the performance of internal combustion engines. ML offers a pathway to transform complex physical processes that occur in a combustion engine into compact informational processes. The developed ML-GGA model was compared with a recently developed Machine Learning-Genetic Algorithm (ML-GA). Detailed investigations of optimization solver parameters and variable limit extension were performed in the present ML-GGA model to improve the accuracy and robustness of the optimization process. Detailed descriptions of the different procedures, optimization tools, and criteria that must be followed for a successful output are provided here. The developed ML-GGA approach was used to optimize the operating conditions (case 1) and the piston bowl design (case 2) of a heavy-duty diesel engine running on a gasoline fuel with a research octane number (RON) of 80. The ML-GGA approach yielded >2% improvements in the merit function, compared with the optimum obtained from a thorough computational fluid dynamics (CFD) guided system optimization. The predictions from the ML-GGA approach were validated with engine CFD simulations. This study demonstrates the potential of ML-GGA to significantly reduce the time needed for optimization problems, without loss in accuracy compared with traditional approaches.

33 ADVANCED PROPULSION SYSTEMS↗

ENGINE COMBUSTION SYSTEM OPTIMIZATION USING CFD AND MACHINE LEARNING: A METHODOLOGICAL APPROACH

Gasoline compression ignition (GCI) engines are considered an attractive alternative to traditional spark-ignition and diesel engines. In this work, a Machine Learning-Grid Gradient Algorithm (ML-GGA) approach was developed to optimize the performance of internal combustion engines. Machine learning (ML) offers a pathway to transform complex physical processes that occur in a combustion engine into compact informational processes. The developed ML-GGA model was compared with a recently developed Machine learning Genetic Algorithm (ML-GA). Detailed investigations of optimization solver parameters and variables limits extension were performed in the present ML-GGA model to improve the accuracy and robustness of the optimization process. Detailed descriptions of the different procedures, optimization tools and criteria that must be followed for a successful output are provided here. The developed ML-GGA approach was used to optimize the operating conditions (case 1) and the piston bowl design (case 2) of a heavy-duty diesel engine running on a gasoline fuel with a Research Octane Number (RON) of 80. The ML-GGA approach yielded > 2% improvements in the merit function, compared to the optimum obtained from a thorough computational fluid dynamics (CFD) guided system optimization. The predictions from the ML-GGA approach were validated with engine CFD simulations. This study demonstrates the potential of ML-GGA to significantly reduce the time needed for optimization problems, without loss in accuracy compared to traditional approaches.

engine optimization↗

Numerical study of advanced compression ignition and combustion in a gasoline direct injection engine

Multi-mode combustion strategies may provide a considerable thermal efficiency improvement targeted at part-load operating conditions for light-duty spark-ignition (SI) engines. The extension from boosted SI mode at high loads to advanced compression ignition (ACI) mode at low loads can be achieved by increasing compression ratio and utilizing intake air heating. In order to enable an accurate control of intake charge condition for ACI control and rapid mode-switches, it is essential to gain fundamental insight into the autoignition process with regard to thermal and fuel-air mixture stratification in the combustion chamber. In this work, a computational fluid dynamics (CFD) study is carried out to reveal some degrees of correlation between the mixture and thermal stratifications induced by the cylinder wall temperature and combustion-phasing across varying engine load conditions. The computational analysis begins with a calibrated simulation setup best matching the engine experiments, and subsequently evaluates the baseline setup for an extended range of engine load conditions for two excess-air ratio cases. The present study emphasizes the dominance of thermal stratifications for autoignition process due to wall temperature effects depending on the engine load conditions of interest. In addition, this study also aims to provide insight into the impact of mixture stratification on cyclic variability, especially at colder wall engine conditions (e.g., cold start). Observations herein provide highlight of the propensity of autoignition in correlation with mixture reactivity space.

Advanced Compression Ignition (ACI) engine↗

Numerical investigation of a fueled pre-chamber spark-ignition natural gas engine

Pre-chamber spark-ignition (PCSI) is a leading advanced ignition concept for internal combustion engines with the potential to enable diesel-like efficiency in medium-duty/heavy-duty (MD/HD) natural gas (NG) engines. By leveraging distributed ignition sources from multiple turbulent jets, the PCSI technology can deliver extremely short combustion duration in ultra-lean mixtures and significantly improve the engine thermal efficiency. However, in the automotive industry there is a lack of adequate science base and predictive simulation tools required for commercial development of PCSI engines. Here, in this study, Reynolds-Average Navier-Stokes simulations are carried out to describe the combustion process in lean-burn NG engines, focusing on the combustion modeling approach. Two combustion models, multi-zone well-stirred reactor (MZ-WSR) and G-equation, are used to simulate the combustion process in an MD NG engine equipped with a fueled-PCSI system for four operating conditions close to the lean operating limit. A skeletal chemical mechanism and a laminar flame speed tabulation are used to compute the combustion accurately. Simulation results are compared with experimental data regarding measured cylinder pressure, heat release rate, and combustion duration. By dividing the PCSI combustion process into four distinct phases, the difference between the two models’ results for each phase is analyzed in detail. The MZ-WSR model overestimates the combustion duration for early flame kernel growth in the pre-chamber due to the lack of a specific formulation to take turbulence-chemistry interaction into account. Despite the prolonged combustion duration and low pressure built-up inside the pre-chamber, the model matches the combustion rate in the main-chamber. In contrast, the G-equation model delivers good agreements for the pre-chamber combustion and turbulent jet-driven combustion processes. However, the model starts to underestimate the combustion rate in the main-chamber, especially under ultra-lean mixture conditions. Finally, improvements are needed for both models to simulate the later combustion stage that occurred in the near-wall regions.

33 ADVANCED PROPULSION SYSTEMS↗

Extreme Miller cycle with high intake boost for improved efficiency and emissions in heavy-duty diesel engines

This study experimentally investigates the impact of extreme Miller cycle strategies paired with high intake manifold pressures on the combustion process, emissions, and thermal efficiency of heavy-duty diesel engines. Well-controlled experiments isolating the effect of Miller cycle strategies on the combustion process were conducted at constant engine speed and load (1160 rpm, 1.76 MPa net IMEP) on a single cylinder research engine equipped with a fully-flexible hydraulic valve train system. Late intake valve closing (LIVC) timing strategies were compared to a conventional intake valve profile under either constant cylinder composition, constant engine-out NO x emission, or constant overall turbocharger efficiency (η TC ) to investigate the operating constraints that favor Miller cycle operation over the baseline strategy. Furthermore, utilizing high boost with conventional intake valve closing timing resulted in improved fuel consumption at the expense of sharp increases in peak cylinder pressures, engine-out NO x emissions, and reduced exhaust temperatures. Miller cycle without EGR at constant λ demonstrated LIVC strategies effectively reduce engine-out NO x emissions by up to 35%. However, Miller cycle associated with very aggressive LIVC timings led to fuel consumption penalties due to increased pumping work and exhaust enthalpy. LIVC strategies allowed for increased charge dilution at the baseline NO x constraint of 3.2 g/kWh, resulting in significant fuel consumption benefits over the baseline case without compromising exhaust temperatures or peak cylinder pressures. As Miller cycle implementation was shown to affect the boundary conditions dictating η TC , the LIVC and conventional IVC cases were studied at an equivalent η TC point representative of high boost operation. With high boost, LIVC yielded reduced NO x emissions, reduced peak cylinder pressures, and elevated exhaust temperatures compared to the conventional IVC case without compromising fuel consumption.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides

BACKGROUND:Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and resources. However, there is a lack of characterized promoters to drive expression of heterologous genes for strain engineering in R. toruloides. RESULTS:This data describes a set of native R. toruloides promoters, characterized over time in four different media commonly used for cultivation of this yeast. The promoter sequences were selected using transcriptional analysis and several of them were found to drive expression bidirectionally. Promoter expression strength was determined by measurement of EGFP and mRuby2 reporters by flow cytometry. A total of 20 constitutive promoters (12 monodirectional and 8 bidirectional) were found, and are expected to be of potential value for genetic engineering of R. toruloides. CONCLUSIONS:A set of robust and constitutive promoters to facilitate genetic engineering of R. toruloides is presented here, ranging from a promoter previously used for this purpose (P7, glyceraldehyde 3-phosphate dehydrogenase, GAPDH) to stronger monodirectional (e.g., P15, mitochondrial adenine nucleotide translocator, ANT) and bidirectional (e.g., P9 and P9R, histones H3 and H4, respectively) promoters. We also identified promoters that may be useful for specific applications such as late-stage expression (e.g., P3, voltage-dependent anion channel protein 2, VDAC2). This set of characterized promoters significantly expands the range of engineering tools available for this yeast and can be applied in future metabolic engineering studies.

09 BIOMASS FUELS↗

CRADA Final Report: Development of Opposed-Piston Variable Compression Ratio Engine for Automotive Applications

Pinnacle Engines, Inc., and researchers at Oak Ridge National Laboratory (ORNL) collaborated under the DOE Small Business Voucher program to perform high-fidelity engine simulations of Pinnacle’s unique opposed-piston engine. In addition to the opposed-piston architecture, Pinnacle’s design includes features such as variable valve timing and compression ratio which provide the potential for improved performance, efficiency, and emissions but also introduce many degrees of freedom in design and operation. The impact and complex interactions of these design and operating parameters must be well understood to direct and optimize the final design. Using DOE’s flagship supercomputing resource, Titan, the team was able to perform an extensive set of numerical engine simulations to explore the impact of these parameters on predicted engine performance. Based on the learnings from this study, Pinnacle will build and experimentally evaluate a prototype engine for light-duty gasoline applications.

42 ENGINEERING↗

International Conference on Microbiome Engineering (ICME 2018)

ICME 2018 explores the importance and applications of microbiome engineering. The conference will focus on engineering of individual organisms in the microbiome as well as the community of organisms enabled by new gene editing tools and computational techniques. This conference brings in experts in industry and academia worldwide to discuss the challenges seen in microbiome engineering and the future of the field. The ICME 2018 conference took place on November 4-6, 2018 at the Joseph B. Martin Conference Center in Boston, MA. The conference consisted of 4 technical sessions: (1) Engineering of the organism and tools to manipulate it; (2) Engineering and modeling of microbial communities; (3) From genome-scale to community-scale models; (4) Computational models for design. There was also a panel discussion regarding the role of engineers in the microbiome field. The keynote and invited speakers were a mix of professionals from both industry and academia.

42 ENGINEERING↗