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At least 1,315 records · Page 73

Ocean time-series near Bermuda: Hydrostation S and the US JGOFS Bermuda Atlantic time-series study

Bermuda is the site of two ocean time-series programs. At Hydrostation S, the ongoing biweekly profiles of temperature, salinity and oxygen now span 37 years. This is one of the longest open-ocean time-series data sets and provides a view of decadal scale variability in ocean processes. In 1988, the U.S. JGOFS Bermuda Atlantic Time-series Study began a wide range of measurements at a frequency of 14-18 cruises each year to understand temporal variability in ocean biogeochemistry. On each cruise, the data range from chemical analyses of discrete water samples to data from electronic packages of hydrographic and optics sensors. In addition, a range of biological and geochemical rate measurements are conducted that integrate over time-periods of minutes to days. This sampling strategy yields a reasonable resolution of the major seasonal patterns and of decadal scale variability. The Sargasso Sea also has a variety of episodic production events on scales of days to weeks and these are only poorly resolved. In addition, there is a substantial amount of mesoscale variability in this region and some of the perceived temporal patterns are caused by the intersection of the biweekly sampling with the natural spatial variability. In the Bermuda time-series programs, we have added a series of additional cruises to begin to assess these other sources of variation and their impacts on the interpretation of the main time-series record. However, the adequate resolution of higher frequency temporal patterns will probably require the introduction of new sampling strategies and some emerging technologies such as biogeochemical moorings and autonomous underwater vehicles.

Michaels, Anthony F.↗

Regional landform thresholds

Remote sensing technology allows us to recognize manifestations of regional thresholds, especially in the spatial characteristics of process agents. For example, a change in river channel pattern over a short distance reflects a threshold alteration in the physical controls of discharge and/or sediment. It is, therefore, a valuable indication of conditions as they exist. However, we probably will have difficulty determining whether the systemic parameters are now close to threshold conditions at which a different change will occur. This, of course, is a temporal and magnitude problem which is difficult to solve from the spatial characteristics.

Ritter, D. F.↗

Method for nanoscale spatial registration of scanning probes with substrates and surfaces

Embodiments in accordance with the present invention relate to methods and apparatuses for aligning a scanning probe used to pattern a substrate, by comparing the position of the probe to a reference location or spot on the substrate. A first light beam is focused on a surface of the substrate as a spatial reference point. A second light beam then illuminates the scanning probe being used for patterning. An optical microscope images both the focused light beam, and a diffraction pattern, shadow, or light backscattered by the illuminated scanning probe tip of a scanning probe microscope (SPM), which is typically the tip of the scanning probe on an atomic force microscope (AFM). Alignment of the scanning probe tip relative to the mark is then determined by visual observation of the microscope image. This alignment process may be repeated to allow for modification or changing of the scanning probe microscope tip.

Wade, Lawrence A.↗

DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage

Outside the magnetic field of the Earth, high energy heavy ions constitute a relevant part of the biologically significant dose to astronauts during the very long travels through space. The typical pattern of energy deposition in the matter by heavy ions on the microscopic scale is believed to produce spatially correlated damage in the DNA which is critical for radiobiological effects. We have investigated the influence of a lucite shielding on the initial production of very small DNA fragments in human fibroblasts irradiated with 1 GeV/u iron (Fe) ions. We also used gamma rays as reference radiation. Our results show: (1) a lower effect per incident ion when the shielding is used; (2) an higher DNA Double Strand Breaks (DSB) induction by Fe ions than by gamma rays in the size range 1-23 kbp; (3) a non-random DNA DSB induction by Fe ions. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.

NASA Discipline Radiation Health↗

Single molecule-based fliFISH validates radial and heterogeneous gene expression patterns in pancreatic islet ß cells

Single cell RNA sequencing (scRNA-Seq) technologies have greatly enhanced our understanding of islet cell transcriptomes and have revealed the existence of ß cell heterogeneity. However, comparison of scRNA-Seq datasets from different groups have highlighted inconsistencies in gene expression patterns, primarily due to variable detection of lower abundance transcripts. Furthermore, such analyses are unable to uncover the spatial organization of heterogeneous gene expression. Here we used fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) to quantify transcripts in single cells in mouse pancreatic islet sections. We compared the expression patterns of Insulin 2 (Ins2) with Mafa and Ucn3 – two genes expressed in ß cells as they mature, as well as Rgs4 – a factor with variably reported expression in the islet. This approach accurately quantified transcripts across a wide range of expression levels - from single copies to over hundred copies per cell in one islet. Importantly, fliFISH allowed evaluation of transcript heterogeneity in the spatial context of an intact islet. These studies confirm the existence of a high degree of heterogeneous gene expression levels within the islet and highlight relative and radial expression patterns that likely reflect distinct ß cell maturation states along the radial axis of the islet.

Li, Fangjia↗

Mitigation of Laser Beam Scintillation in Free-Space Optical Communication Systems Through Coherence-Reducing Optical Materials

Free-space optical communication systems (also known as lasercom systems) offer several performance advantages over traditional radio frequency communication systems. These advantages include increased data rates and reduced operating power and system weight. One serious limiting factor in a lasercom system is Optical turbulence in Earth's atmosphere. This turbulence breaks up the laser beam used to transmit the information into multiple segments that interfere with each other when the beam is focused onto the receiver. This interference pattern at the receiver changes with time causing fluctuations in the received optical intensity (scintillation). Scintillation leads to intermittent losses of the signal and an overall reduction in the lasercom system's performance. Since scintillation is a coherent effect, reducing the spatial and temporal coherence of the laser beam will reduce the scintillation. Transmitting a laser beam through certain materials is thought to reduce its coherence. Materials that were tested included: sapphire, BK7 glass, fused silica and others. The spatial and temporal coherence of the laser beam was determined by examining the interference patterns (fringes) it formed when interacting with various interferometers and etalons.

Renner, Christoffer J.↗

Optical Imaging of Flow Pattern and Phantom

Time-resolved optical imaging technique has been used to image the spatial distribution of small droplets and jet sprays in a highly scattering environment. The snake and ballistic components of the transmitted pulse are less scattered, and contain direct information about the sample to facilitate image formation as opposed to the diffusive components which are due to multiple collisions as a light pulse propagates through a scattering medium. In a time-gated imaging scheme, these early-arriving, image-bearing components of the incident pulse are selected by opening a gate for an ultrashort period of time and a shadowgram image is detected. Using a single shot cooled CCD camera system, the formation of water droplets is monitored as a function of time. Picosecond time-gated image of drop in scattering cells, spray droplets as a function of let speed and gas pressure, and model calcification samples consisted of calcium carbonate particles of irregular shapes ranging in size from 0. 1 to 1.5 mm affixed to a microscope slide have been measured. Formation produced by an impinging jet will be further monitored using a CCD with 1 kHz framing illuminated with pulsed light. The desired image resolution of the fuel droplets is on the 20 pm scale using early light through a highly scattering medium. A 10(exp -6)m displacement from a jet spray with a flow speed of 100 m/sec introduced by the ns grating pulse used in the imaging is negligible. Early ballistic/snake light imaging offers nondestructive and noninvasive method to observe the spatial distribution of hidden objects inside a highly scattering environment for space, biomedical, and materials applications. In this paper, the techniques we will present are time-resolved K-F transillumination imaging and time-gated scattered light imaging. With a large dynamic range and high resolution, time-gated early light imaging has the potential for improving rocket/aircraft design by determining jets shape and particle sizes. Refinements to these techniques may enable drop size measurements in the highly scattering, optically dense region of multi-element rocket injectors. These types of measurements should greatly enhance the design of stable, and higher performing rocket engines.

Galland, Pierre A.↗

Theories of Simplification and Scaling of Spatially Distributed Processes

The problem of scaling is at the heart of ecological theory, the essence of understanding and of the development of a predictive capability. The description of any system depends on the spatial, temporal, and organizational perspective chosen; hence it is essential to understand not only how patterns and dynamics vary with scale, but also how patterns at one scale are manifestations of processes operating at other scales. Evolution has shaped the characteristics of species in ways that result in scale displacement: Each species experiences the environment at its own unique set of spatial and temporal scales and interfaces the biota through unique assemblages of phenotypes. In this way, coexistence becomes possible, and biodiversity is enhanced. By averaging over space, time, and biological interactions, a genotype filters variation at fine scales and selects the arena in which it will face the vicissitudes of nature. Variation at finer scales is then noise, of minor importance to the survival and dynamics of the species, and consequently of minor importance in any attempt at description. In attempting to model ecological interactions in space, contributors throughout this book have struggled with a trade-off between simplification and "realistic" complexity and detail. Although the challenge of simplification is widely recognized in ecology, less appreciated is the intertwining of scaling questions and scaling laws with the process of simplification. In the context of this chapter simplification will in general mean the use of spatial or ensemble means and low-order moments to capture more detailed interactions by integrating over given areas. In this way, one can derive descriptions of the system at different spatial scales, which provides the essentials for the extraction of scaling laws by examination of how system properties vary with scale.

Levin, Simon A.↗

Patterns of megafloral change across the Cretaceous-Tertiary boundary in the Northern Great Plains and Rocky Mountains

The spatial and temporal distribution of vegetation in the terminal Cretaceous of Western Interior North America was a complex mosaic resulting from the interaction of factors including a shifting coastline, tectonic activity, a mild, possibly deteriorating climate, dinosaur herbivory, local facies effects, and a hypothesized bolide impact. In order to achieve sufficient resolution to analyze this vegetational pattern, over 100 megafloral collecting sites were established, yielding approximately 15,000 specimens, in Upper Cretaceous and lower Paleocene strata in the Williston, Powder River, and Bighorn basins in North Dakota, Montana, and Wyoming. These localities were integrated into a lithostratigraphic framework that is based on detailed local reference sections and constrained by vertebrate and palynomorph biostratigraphy, magnetostratigraphy, and sedimentary facies analysis. A regional biostratigraphy based on well located and identified plant megafossils that can be used to address patterns of floral evolution, ecology, and extinction is the goal of this research. Results of the analyses are discussed.

Johnson, Kirk R.↗

Exploring the Spatial Relationship Between Demographic Indicators and the Built Environment of a City

In addition to global and regional drivers of urbanization, neighborhood development in urban areas across the United States has been shown to be influenced by various local socio-economic factors. These factors, despite varying across socio-economic groups, have large implications regarding a population’s vulnerability to extreme climate events, including heat waves resulting in adverse health impacts. Additionally, the demographics of an urban area can shape its infrastructural characteristics, causing different populations groups to face varying levels of risks and benefits. As a result, the urban morphology and socio-economic characteristics of a city are deeply intertwined; however, their interactions on a finer scale are not yet fully understood. This research aims to better understand the relationships between various socio-economic factors and the built environment of a city, considering variability in building types, and temperature patterns. This research focuses on the city of Las Vegas, NV, and uses spatial data analysis to understand the correlation between of socio-economic characteristics, building morphology, building characteristics, and temperature data to understand the correlation between these various factors. Results of these research shows there is a distinct pattern of clustering of socio-economic characteristics with the city and there is a distinct correlation between age and cost, socio-economic characteristics, and locations of high heat distribution within the city.

Singh, Ridhima↗

Patterns in simulated turbulent channel flow

The surface stress is a key diagnostic in wall bounded turbulent flows. Large fluctuations in the stress are believed to be associated with intermittent 'bursting' events during which a large proportion of the turbulence production takes place. If this is so, then a detailed investigation of the structure of the surface stress and its spatial relationship to events within the flow could have wide application in drag reduction and other aspects of flow control. The initial phase of this project, therefore, concentrated on the surface stress field. The first objective is to carry out a statistical analysis of the instantaneous surface stress in a simulated turbulent channel flow, including comparison with multipoint experimental data from a zero pressure gradient turbulent boundary layer. The second objective is to apply a simple pattern recognition procedure to educe the characteristic spatial structure of various flow diagnostics. The final objective is to extend the pattern recognition analysis to examine the whole three dimensional structure of the flow.

Stretch, D.↗

Charging Reactions Promoted by Geometrically Necessary Dislocations in Battery Materials Revealed by In Situ Single-Particle Synchrotron Measurements

Crystallographic defects exist in many redox active energy materials, e.g., battery and catalyst materials, which significantly alter their chemical properties for energy storage and conversion. However, there is lack of quantitative understanding of the interrelationship between crystallographic defects and redox reactions. Herein, crystallographic defects, such as geometrically necessary dislocations, are reported to influence the redox reactions in battery particles through single-particle, multimodal, and in situ synchrotron measurements. Through Laue X-ray microdiffraction, many crystallographic defects are spatially identified and statistically quantified from a large quantity of diffraction patterns in many layered oxide particles, including geometrically necessary dislocations, tilt boundaries, and mixed defects. The in situ and ex situ measurements, combining microdiffraction and X-ray spectroscopy imaging, reveal that LiCoO 2 particles with a higher concentration of geometrically necessary dislocations provide deeper charging reactions, indicating that dislocations may facilitate redox reactions in layered oxides during initial charging. The present study illustrates that a precise control of crystallographic defects and their distribution can potentially promote and homogenize redox reactions in battery materials.

25 ENERGY STORAGE↗

Spring Regional Sea Surface Temperatures as a Precursor of European Summer Heatwaves

Different spring and early summer North Atlantic sea surface temperature anomalies (SSTAs) have been shown to precede recent European summer heatwaves (EuSHWs). So far, the limited number of observed events associated with several physical mechanisms has prevented a robust identification of SSTAs as precursors. Here, we extend beyond previous studies by combining 100 historical simulations (1850–2005) of the MPI Grand-Ensemble with an explainable neural-network method. We find that the spring tripolar North Atlantic pattern with positive SSTAs in the Subtropical Gyre is a precursor of EuSHWs. In addition, positive SSTAs west of the Iberian Peninsula, and in the North Sea, the Baltic Sea and the Mediterranean Sea relate to distinct early summer soil moisture anomaly patterns and are precursors of western and southeastern EuSHWs, respectively. While the phase of the tripolar North Atlantic pattern indicates whether a EuSHW might emerge, regional SSTAs indicate the spatial characteristics of EuSHWs.

54 ENVIRONMENTAL SCIENCES↗

Superconducting diode effect via conformal-mapped nanoholes

A superconducting diode is an electronic device that conducts supercurrent and exhibits zero resistance primarily for one direction of applied current. Such a dissipationless diode is a desirable unit for constructing electronic circuits with ultralow power consumption. However, realizing a superconducting diode is fundamentally and technologically challenging, as it usually requires a material structure without a centre of inversion, which is scarce among superconducting materials. Here, we demonstrate a superconducting diode achieved in a conventional superconducting film patterned with a conformal array of nanoscale holes, which breaks the spatial inversion symmetry. We showcase the superconducting diode effect through switchable and reversible rectification signals, which can be three orders of magnitude larger than that from a flux-quantum diode. The introduction of conformal potential landscapes for creating a superconducting diode is thereby proven as a convenient, tunable, yet vastly advantageous tool for superconducting electronics. This could be readily applicable to any superconducting materials, including cuprates and iron-based superconductors that have higher transition temperatures and are desirable in device applications.

36 MATERIALS SCIENCE↗

Particle emission with identification from an atmospheric pressure plasma liquid interface

A conspicuous emission phenomenon of luminous particles from the liquid anode surface of an atmospheric pressure DC glow discharge is reported. The emission has been shown to occur when the plasma forms a pattern on the surface of the liquid electrolyte. Here, the spatial-temporal evolutions of the trajectories of emitted particles were studied using a high-speed imaging system. Particles were sampled in flight using a witness plate for analysis. Critical particle characteristics were examined using electron microscope technologies. The morphology of the resulting splats and compositions suggest that they are molten droplets with a great deal of structure, including evidence of nanoprecipitation. A theoretical model was employed to estimate the particle size from the measured splats, which provides a radius range within a hundred micrometers. This experiment leads to the postulation of a mechanism in which particle emission at the plasma liquid interface is likely driven by the Taylor cone effect.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

An uncertainty-aware strategy for plasma mechanism reduction with directed weighted graphs

In this work, we present a framework for the analysis and reduction of plasma mechanisms by means of weighted directed graphs, in which reactions and species are both treated as nodes. The methodology consists of two distinct analyses. The first, which is qualitative, relies on graph spatializations via force-directed algorithms to discover the predominant global patterns in the chemical model. The second ranks the reactions based on their shortest paths' lengths from/to the species of interest and their relative contributions to the power balance. Further, this quantitative investigation enables a strategy for mechanism reduction that is fully automatized, as it does not require any expert knowledge, highly effective, as it generates reduced mechanisms that are highly accurate while relying on a small number of processes, and easily interpretable, as the algorithm justifies the importance of the retained reactions by outputting their related chemical pathways. Additionally, the work proposes a methodology extension that employs ensembles of graphs to improve the robustness of the reduced mechanism to reaction parameter uncertainties. The approach, here tested for steady-state predictions of a plasma system characterizing negative hydrogen ion sources, is general and can be used in a wide variety of applications outside the particular nuclear fusion context demonstrated in this work.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Plant-microbe interactions: from genes to ecosystems using Populus as a model system

Plant-microbe symbioses span a continuum from pathogenic to mutualistic with functional consequences for both organisms in the symbiosis. In order to increase sustainable food and fuel production in the future, it is imperative that we harness these symbioses. The tree genus Populus is an excellent model system for studies examining plant-microbe interactions due to the wealth of genomic information available and the molecular tools that have been developed to manipulate Populus-microbe symbioses. In this review, we highlight how Populus can serve as a model system to explore plant-microbe interactions. Specifically, highlighting research linking Populus-microbe interactions from the gene to the ecosystem level. We explore why Populus is an excellent model for perennial plant systems, the molecular underpinnings of Populus-microbe interactions, how host genetics influence microbial community composition, and how microbial communities vary at fine spatial scales and between Populus species. Further, we explore how the patterns of the microbiome may affect ecosystem level functions in managed and natural ecosystems. Understanding and manipulating these interactions in Populus has the potential to improve plant health and impact ecosystem sustainability and processes as Populus trees function as foundational species in many natural ecosystems and are also deployed in managed ecosystems for various agroforestry applications.

59 BASIC BIOLOGICAL SCIENCES↗

Local Ferromagnetic Resonance Measurements of Mesoscopically Patterned Ferromagnets Using Deterministically Placed Nanodiamonds

Nitrogen-vacancy centers in diamond have recently been established as effective sensors of the magnetization dynamics in vicinal ferromagnetic materials. Here, we demonstrate sub-100-nm placement accuracy of nitrogen-vacancy-containing nanodiamonds and use these as local sensors that probe optically detected ferromagnetic resonance in mesoscopically patterned Permalloy islands. These measurements reveal variations in the ferromagnetic resonance signal at different sites on these structures with distinct behavior in the edge and the bulk of patterned features. These test measurements establish an easily implemented approach for spatially targeted measurements of spin dynamics in mesoscale ferromagnets. In principle, the methodology can also be extended to local studies of nanoscale ferromagnets, such as single magnetic nanowires and nanoparticles.

30 DIRECT ENERGY CONVERSION↗