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At least 1,315 records · Page 73

From primordial clocks to circadian oscillators

Circadian rhythms play an essential part in many biological processes, and only three prokaryotic proteins are required to constitute a true post-translational circadian oscillator1. The evolutionary history of the three Kai proteins indicates that KaiC is the oldest member and a central component of the clock. Subsequent additions of KaiB and KaiA regulate the phosphorylation state of KaiC for time synchronization. The canonical KaiABC system in cyanobacteria is well understood but little is known about more ancient systems that only possess KaiBC. However, there are reports that they might exhibit a basic, hourglass-like timekeeping mechanism. Here we investigate the primordial circadian clock in Rhodobacter sphaeroides , which contains only KaiBC, to elucidate its inner workings despite missing KaiA. Using a combination of X-ray crystallography and cryogenic electron microscopy, we find a new dodecameric fold for KaiC, in which two hexamers are held together by a coiled-coil bundle of 12 helices. This interaction is formed by the carboxy-terminal extension of KaiC and serves as an ancient regulatory moiety that is later superseded by KaiA. A coiled-coil register shift between daytime and night-time conformations is connected to phosphorylation sites through a long-range allosteric network that spans over 140 Å. Our kinetic data identify the difference in the ATP-to-ADP ratio between day and night as the environmental cue that drives the clock. They also unravel mechanistic details that shed light on the evolution of self-sustained oscillators.

59 BASIC BIOLOGICAL SCIENCES↗

Physical origin of higher-order soliton fission in nanophotonic semiconductor waveguides

Abstract Supercontinuum generation in Kerr media has become a staple of nonlinear optics. It has been celebrated for advancing the understanding of soliton propagation as well as its many applications in a broad range of fields. Coherent spectral broadening of laser light is now commonly performed in laboratories and used in commercial “white light” sources. The prospect of miniaturizing the technology is currently driving experiments in different integrated platforms such as semiconductor on insulator waveguides. Central to the spectral broadening is the concept of higher-order soliton fission. While widely accepted in silica fibers, the dynamics of soliton decay in semiconductor waveguides is yet poorly understood. In particular, the role of nonlinear loss and free carriers, absent in silica, remains an open question. Here, through experiments and simulations, we show that nonlinear loss is the dominant perturbation in wire waveguides, while free-carrier dispersion is dominant in photonic crystal waveguides.

42 ENGINEERING↗

Lagrangian statistics of a shock-driven turbulent dynamo in decaying turbulence

Small-scale fluctuating magnetic fields of order nG are observed in supernova shocks and galaxy clusters, where its amplification is likely caused by the Biermann battery mechanism. However, these fields cannot be amplified further without the turbulent dynamo, which generates magnetic energy through the stretch-twist-fold (STF) mechanism. Thus, we present here novel 3D magnetohydrodynamic (MHD) simulations of a laser-driven shock propagating into a stratified, multiphase medium, to investigate the post-shock turbulent magnetic field amplification via the turbulent dynamo. The configuration used here is currently being tested in the shock tunnel at the National Ignition Facility (NIF). In order to probe the statistical properties of the post-shock turbulent region, we use 384 × 512 × 384 tracers to track its evolution through the Lagrangian framework, thus providing a high-fidelity analysis of the shocked medium. Our simulations indicate that the growth of the magnetic field, which accompanies the near-Saffman kinetic energy decay (E kin ∝ t -1.15 ) without turbulence driving, exhibits slightly different characteristics as compared to periodic box simulations. Seemingly no distinct phases exist in its evolution because the shock passage and time to observe the magnetic field amplification during the turbulence decay are very short (~0.3 of a turbulent turnover time). Yet, the growth rate is still consistent with those expected for compressive (curl-free) turbulence driving in subsonic, compressible turbulence. Phenomenological understanding of the dynamics of the magnetic and velocity fields are also elucidated via Lagrangian frequency spectra, which are consistent with the expected inertial range scalings in the Eulerian–Lagrangian bridge.

79 ASTRONOMY AND ASTROPHYSICS↗

Tunable, Ultrafast Fiber-Laser between 1.15 μm and 1.35 μm for Harmonic Generation Microscopy in Human Skin

We demonstrate a fiber-optic ultrafast tunable source for harmonic generation microscopy (HGM) in human skin. The source is based on a 31-MHz Er-fiber laser followed by self-phase modulation enabled spectral selection (SESS). The resulting pulses are tunable between 1.15 and 1.35 μm with up to >10-nJ pulse energy and ~100-fs pulse duration. Here we employ this source to drive a scanning microscope for HGM imaging of ex vivo human skin. A systematic investigation on imaging depth versus excitation wavelength reveals that excitation wavelengths in the 1.15-1.25 μm range exhibit low optical attenuation within the tissue and allows larger imaging depth for HGM in human skin. HGM driven by fiber-based SESS sources constitutes an enabling tool for noninvasive virtual skin biopsy in clinical applications.

60 APPLIED LIFE SCIENCES↗

Structural Principles in Liquids and Glasses: Bottom-Up or Top-Down

The conventional approach to elucidate the atomic structure of liquid and glass is to start with local structural units made of several atoms, and to use them as building blocks to form a global structure, the bottom-up approach. We propose to add an alternative top-down approach in which we start with a global high-temperature gas state and then apply interatomic potentials to all atoms at once. This causes collective density wave instability in all directions with the same wavelength. These two driving forces, local and global, are in competition and are mutually frustrated. The final structure is determined through the compromise of frustration between these two, which creates the medium-range-order. This even-handed approach on global and local potential energy landscapes explains the distinct natures of short-range order and medium-range order, and strong temperature dependence of various properties of liquid.

36 MATERIALS SCIENCE↗

The relationship between molecular gas, H I, and star formation in the low-mass, low-metallicity magellanic clouds

The Magellanic Clouds provide the only laboratory to study the effects of metallicity and galaxy mass on molecular gas and star formation at high (∼⃒20 pc) resolution. We use the dust emission from HERITAGE Herschel data to map the molecular gas in the Magellanic Clouds, avoiding the known biases of CO emission as a tracer of H{sub 2}. Using our dust-based molecular gas estimates, we find molecular gas depletion times (τ{sub dep}{sup mol}) of ∼⃒0.4 Gyr in the Large Magellanic Cloud and ∼⃒0.6 in the Small Magellanic Cloud at 1 kpc scales. These depletion times fall within the range found for normal disk galaxies, but are shorter than the average value, which could be due to recent bursts in star formation. We find no evidence for a strong intrinsic dependence of the molecular gas depletion time on metallicity. We study the relationship between the gas and the star formation rate across a range of size scales from 20 pc to ≧̸1 kpc, including how the scatter in τ{sub dep}{sup mol} changes with the size scale, and discuss the physical mechanisms driving the relationships. We compare the metallicity-dependent star formation models of Ostriker et al. and Krumholz to our observations and find that they both predict the trend in the data, suggesting that the inclusion of a diffuse neutral medium is important at lower metallicity.

79 ASTRONOMY AND ASTROPHYSICS↗

A linear accelerator for simulated micrometeors.

Review of the theory, design parameters, and construction details of a linear accelerator designed to impart meteoric velocities to charged microparticles in the 1- to 10-micron diameter range. The described linac is of the Sloan Lawrence type and, in a significant departure from conventional accelerator practice, is adapted to single particle operation by employing a square wave driving voltage with the frequency automatically adjusted from 12.5 to 125 kHz according to the variable velocity of each injected particle. Any output velocity up to about 30 km/sec can easily be selected, with a repetition rate of approximately two particles per minute.

Slattery, J. C.↗

Testing of the permanent magnet material Mn-Al-C for potential use in propulsion motors for electric vehicles

The development of Mn-Al-C permanent magnets is reviewed. The general properties of the material are discussed and put into perspective relative to alnicos and ferrites. The traction motor designer's demands of a permanent magnet for potential use in electric vehicle drives are reviewed. Tests determined magnetic design data and mechanical strength properties. Easy axis hysteresis and demagnetization curves, recoil loops and other minor loop fields were measured over a temperature range from -50 to 150 C. Hysteresis loops were also measured for three orthogonal directions (the one easy and two hard axes of magnetization). Extruded rods of three different diameters were tested. The nonuniformity of properties over the cross section of the 31 mm diameter rod was studied. Mechanical compressive and bending strength at room temperature was determined on individual samples from the 31 mm rod.

Abdelnour, Z.↗

Integrated computer-aided design using minicomputers

Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM), a highly interactive software, has been implemented on minicomputers at the NASA Langley Research Center. CAD/CAM software integrates many formerly fragmented programs and procedures into one cohesive system; it also includes finite element modeling and analysis, and has been interfaced via a computer network to a relational data base management system and offline plotting devices on mainframe computers. The CAD/CAM software system requires interactive graphics terminals operating at a minimum of 4800 bits/sec transfer rate to a computer. The system is portable and introduces 'interactive graphics', which permits the creation and modification of models interactively. The CAD/CAM system has already produced designs for a large area space platform, a national transonic facility fan blade, and a laminar flow control wind tunnel model. Besides the design/drafting element analysis capability, CAD/CAM provides options to produce an automatic program tooling code to drive a numerically controlled (N/C) machine. Reductions in time for design, engineering, drawing, finite element modeling, and N/C machining will benefit productivity through reduced costs, fewer errors, and a wider range of configuration.

Storaasli, O. O.↗

An experimental data base for material selection and design of high-speed, high-pressure, oxygen turbomachinery

New technologies for space-based, reusable, throttleable, cryogenic, orbit transfer propulsion are being evaluated. A variable-thrust (200 to 3000 lbF), 2000 psi chamber pressure, LO2/LH2 engine has been selected to demonstrate the 20-hour, 500-restart life goal, and a specific impulse of 480 lbF-sec/lbM. The advanced design uses warm oxygen to power a fully integrated turbopump that delivers 4500 psi LO2. The selected engine cycle provides the following advantages over conventional expander cycle designs which use H2 to drive the LH2 and LO2 pumps; higher engine operating pressures and performance in a smaller envelope, lower turbine operating temperatures, elimination of interpropellant seals and purges, and an extended throttling range. The design approach and results of testing to characterize materials for use in high-pressure gaseous oxygen are discussed. Test methods include particle impact testing in a sonic-flow, hot GO2 streams and forced, high-speed friction rubbing testing. Materials are exposed to oxygen and nitrogen atmospheres at pressures of 100, 1000, and 3000 psi to identify separately the gas cooling effects from the metal oxidation effects at the rubbing surface. The selection of candidate design materials is based on an analytical parameter defined as the burn factor. Typical materials tested include carbon steel, stainless steel, nickel, copper, and monel alloys.

Schoenman, L.↗

Analysis of 7- X 10-foot high speed wind tunnel shaft loads in support of fan blade failure investigation

This is a report of the investigation of the High-Speed 7- X 10-Foot Wind Tunnel at NASA Langley Research Center, which experienced a catastrophic failure of all 18 Sitka spruce fan blades during operation at 0.8 Mach number on 2 July 1985. The High-Speed Tunnel, a closed-circuit/single-return atmospheric wind tunnel, had been operated since 1945 to support a wide range of subsonic aerodynamic tests and studies. The failed blade set had been in use since 1975. In addition to blade loss, the most significant damage was a bent main drive shaft for a total estimated damage loss of 1.7 million dollars. An analysis of the natural frequency characteristics as well as loads, reactions, stresses, and deflections of the fan drive system resulting from steady-state and dynamic loads due to unbalance was performed. Transient load cases were simulated by step input and ramp input loading functions intended to simulate the loss of one to nine blades (maximum unbalance forces).

Faison, Richard W.↗

Flight Investigation of an Automatic Pitchup Control

A flight investigation of an automatic pitchup control has been conducted by the National Aeronautics and Space Administration at the Langley Research Center. The pitching-moment characteristics of a transonic fighter airplane which was subject to pitchup were altered by driving the stabilizer in accordance with a signal that was a function of a combination of the measured angle of attack and the pitching velocity. An angle-of-attack threshold control was used to preset the angle of attack at which the automatic pitchup-control system would begin to drive the stabilizer. No threshold control as such existed for the pitching-velocity signal. A summing linkage in series with the pilot's longitudinal control allowed the automatic pitchup-control system to drive the stabilizer 13.5 percent of the total stabilizer travel independently of the pilot's control. Tests were made at an altitude of 35,000 feet over a Mach number range of 0.80 to 0.90. Various gearings between the control and the sensing devices were investigated. The automatic system was capable of extending the region of positive stability for the test airplane to angles of attack above the basic-airplane pitchup threshold angle of attack. In most cases a limit-cycle oscillation about the airplane pitch axis occurred.

Hurt, George J., Jr.↗

Use of an Ultrasonic/Sonic Driller/Corer to Obtain Sample Powder for CHEMIN, a Combined XRD/XRF Instrument

A miniature CHEMIN XRD/XRF (X-Ray Diffraction/X-Ray Fluourescence) instrument is currently being developed for definitive mineralogic analysis of soils and rocks on Mars. One of the technical issues that must be addressed in order to enable XRD analysis on an extraterrestrial body is how best to obtain a representative sample powder for analysis. For XRD powder diffraction analyses, it is beneficial to have a fine-grained sample to reduce preferred orientation effects and to provide a statistically significant number of crystallites to the X-ray beam. Although a 2-dimensional detector as used in the CHEMIN instrument will produce good results with poorly prepared powders, the quality of the data will improve if the sample is fine-grained and randomly oriented. An Ultrasonic/Sonic Driller/Corer (USDC) currently being developed at JPL is an effective mechanism of sampling rock to produce cores and powdered cuttings. It requires low axial load (< 5N) and thus offers significant advantages for operation from lightweight platforms and in low gravity environments. The USDC is lightweight (<0.5kg), and can be driven at low power (<5W) using duty cycling. It consists of an actuator with a piezoelectric stack, ultrasonic horn, free-mass, and drill bit. The stack is driven with a 20 kHz AC voltage at resonance. The strain generated by the piezoelectric is amplified by the horn by a factor of up to 10 times the displacement amplitude. The tip impacts the free-mass and drives it into the drill bit in a hammering action. The free-mass rebounds to interact with the horn tip leading to a cyclic rebound at frequencies in the range of 60-1000 Hz. It does not require lubricants, drilling fluid or bit sharpening and it has the potential to operate at high and low temperatures using a suitable choice of piezoelectric material. To assess whether the powder from an ultrasonic drill would be adequate for analyses by an XRD/XRF spectrometer such as CHEMIN, powders obtained from the JPL ultrasonic drill were analyzed and the results were compared to carefully prepared powders obtained using a laboratory bench scale Retsch mill.

Chipera, S. J.↗

The back photoreaction of the M intermediate in the photocycle of bacteriorhodopsin: mechanism and evidence for two M species

The back photoreaction of the M intermediate in the photocycle of bacteriorhodopsin is investigated both for the native pigment and its D96N mutant. The experimental setup is based on creating the M intermediate by a first pulse, followed by a (blue) laser pulse which drives the back photoreaction of M. Experiments are carried out varying the delay between the two pulses, as well as the temperature over the -25 degrees C-20 degrees C range. It is found that the kinetic patterns of the M back photoreaction change with time after the generation of this intermediate. The data provide independent evidence for the suggestion of a photocycle mechanism based on two distinct M intermediates. They are thus in keeping with the consecutive model of Varo and Lanyi (Biochemistry 30, 5016-5022; 1991), although they cannot exclude other models such as those based on branched or parallel cycles. More generally, we offer a "photochemical" approach to discriminating between intermediate stages in the photocycle which does not depend on spectroscopic and/or kinetic data. While markedly affecting the rate of the M --> N transition in the photocycle, the rate of the thermal step in back photoreaction of M, at both room and low temperatures, is not significantly affected by the D96N mutation. It is proposed that while Asp 96 is the Schiff-base protonating moiety in the M --> N transition, another residue (most probably Asp 85) reprotonates the Schiff base following light absorption by M.

NASA Discipline Exobiology↗

Precision Linear Actuators for the Spherical Primary Optical Telescope Demonstration Mirror

The Spherical Primary Optical Telescope (SPOT) is an ongoing research effort at Goddard Space Flight Center developing wavefront sensing and control architectures for future space telescopes. The 03.5 m SPOT telescope primary mirror is comprised20f 6, 0.86 m hexagonal mirror segments arranged in a single ring, with the central segment missing . The mirror segments are designed for laboratory use and are not light-weighted to reduce cost. Each primary mirror segment is actuated and has tip, tilt, and piston rigid body motions. Additionally, the radius of curvature of each mirror segment may be varied mechanically3. To provide these degrees of freedom, the SPOT mirror segment assembly requires linear actuators capable of 10 nm resolution over a total stroke of 5 mm. These actuators must withstand high static loads as they must support the mirror segment, which has a mass of approx.100 kg. A stepper motor driving a differential satellite roller screw was designed to meet these demanding requirements. Initial testing showed that the actuator is capable of sub-micron repeatability over the entire 6mm range, and was limited by 100-200 nm measurement noise levels present in the facility. Further testing must be accomplished in an isolated facility with a measurement noise floor of < 5 nm. Such a facility should be ready for use at GSFC in the early summer of 2006, and will be used to better characterize this actuator.

Budinoff, Jason↗

Influence of Load Profile on Biomechanics of the Squat and Deadlift

Long duration space travel will expose astronauts to extended periods of reduced gravity. Since gravity is not present to assist loading, astronauts will use resistive and aerobic exercise regimes for the duration of the space flight to counteract the effect reduced gravity has on the body. Astronauts will exercise on a flywheel based device on the second Orion Exploration Mission (EM2). The effect that the flywheel load profile has on biomechanics is unknown when compared to free weights or a simulated free weight profile. The purpose of this evaluation is to compare the differences in lower body kinematics and kinetics between the flywheel and free weight profile. Test subjects were instrumented with reflective markers for motion capture data collection while exercising on the Device for Aerobic and Resistive Training (DART) prototype developed by TDA Research, Inc. of Wheat Ridge, CO. Exercises performed were either the squat while wearing a harness or deadlift while grasping a T-bar, both of which interfaced with the DART through a rope cable. The DART, a motorized device controlled via software, is capable of producing different load profiles. Profiles include simulated free weights with adjustable eccentric overload capability and flywheel. Test variables included the applied device load and the load profile, both set through the DART software interface. Motion capture data was collected with a 12 camera system (Smart-DX, BTS Bioengineering, Brooklyn, NY). Bilateral ground reaction force data were collected with force plates (P6000, BTS Bioengineering). DART cable force was recorded through an internal load cell. Data was collected from a total of four test subjects. The three repetition maximum was determined for each test subject for both squat and deadlift. High, medium and low loads were determined based on this maximum. The test subject performed five repetitions of each exercise at each load and each test trial was repeated twice. Cadence was controlled during exercising. Biomechanical data were used to drive the models utilizing the OpenSim software platform. Subject specific models were scaled to match the anthropometrics of the test subjects and used to estimate the peak joint angle, joint range of motion, the peak joint moment and the joint moment impulse of the lower extremity joints and the lumbar joint.These are preliminary results because the data analysis is ongoing. There was a lot of inter-subject variability, however, in general, there was a greater peak lumbar flexion angle for the flywheel squat exercise. There was a trend towards a greater range of motion at the hip for the squat exercise with free weight. There was also a greater hip extension, lumbar extension and ankle plantar flexion impulse moment during the squat exercise with free weight. During the deadlift exercise, the peak knee flexion angle and peak knee extension moment were greater when using the free weight profile. For deadlift, the hip extension, lumbar extension and ankle plantar flexion impulse moment tended to be greater with the free weight profile. Overall, the kinematic and kinetic outcomes for the flywheel profile were either statistically the same as free weight profile in many cases, significantly reduced in a few cases, and rarely higher.

Bioengineering↗

Origin of the multiple configurations that drive the response of δ-plutonium’s elastic moduli to temperature

Significance Our measurements have shown that the face-centered cubic (δ)-phase of plutonium exhibits little or no volume change but softens drastically as temperature rises over a very broad range. This behavior constrains any microscopic theory of plutonium to require that more than one thermodynamically accessible configuration exists, that the higher energy one has a smaller volume, and contrary to most models, the higher energy, smaller volume configuration must be more compressible. Until the recent neutron-scattering discovery of dynamic magnetism in plutonium, no evidence for such configurations existed. With that discovery, we are able to justify our microscopic theory that captures the observed neutron-scattering and temperature behavior of δ-plutonium.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Ion drive performance and trajectories

The use of Solar Electric Propulsion (SEP) for the Solar Probe Mission is addressed. The dependence of the payload mass on the site of the SEP and the flight time are described on the basis of preliminary data. The range of hardware expected to be available in the 1980's is summarized. There are several classes of optimal low-thrust trajectories for the Solar Probe Mission. These are trajectory types A, B, C corresponding to one, two, or three orbital revolutions, respectively. Plots of transfer trajectories corresponding to type-A, type-A with a Venus Swingby, type-B, and type-C with ab Icarus Rendezvous are shown. A summary of the SEP performance is given.

Yen, C. L.↗