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At least 1,315 records · Page 73

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DOE OSTI2020

Nanostructural Analysis of Enzymatic and Non-enzymatic Brown Rot Fungal Deconstruction of the Lignocellulose Cell Wall†

Brown rot (BR) decay mechanisms employ carbohydrate-active enzymes (CAZymes) as well as a unique non-enzymatic chelator-mediated Fenton (CMF) chemistry to deconstruct lignocellulosic materials. Unlike white rot fungi, BR fungi lack peroxidases for lignin deconstruction, and also lack some endoglucanase/cellobiohydrolase activities. The role that the CMF mechanism plays in “opening up” the wood cell wall structure in advance of enzymatic action, and any interaction between CMF constituents and the selective CAZyme suite that BRs possess, is still unclear. Expression patterns for CMF redox metabolites and lytic polysaccharide monooxygenase (LPMO–AA9 family) genes showed that some LPMO isozymes were upregulated with genes associated with CMF at early stages of brown rot by Gloeophyllum trabeum. In the structural studies, wood decayed by the G. trabeum was compared to CMF-treated wood, or CMF-treated wood followed by treatment with either the early-upregulated LPMO or a commercial CAZyme cocktail. Structural modification of decayed/treated wood was characterized using small angle neutron scattering. CMF treatment produced neutron scattering patterns similar to that of the BR decay indicating that both systems enlarged the nanopore structure of wood cell walls to permit enzyme access. Enzymatic deconstruction of cellulose or lignin in raw wood samples was not achieved via CAZyme cocktail or LPMO enzyme action alone. CMF treatment resulted in depolymerization of crystalline cellulose as attack progressed from the outer regions of individual crystallites. Multiple pulses of CMF treatment on raw wood showed a progressive increase in the spacing between the cellulose elementary fibrils (EFs), indicating the CMF eroded the matrix outside the EF bundles, leading to less tightly packed EFs. Peracetic acid delignification treatment enhanced subsequent CMF treatment effects, and allowed both enzyme systems to further increase spacing of the EFs. Moreover, even after a single pulse of CMF treatment, both enzymes were apparently able to penetrate the cell wall to further increase EF spacing. The data suggest the potential for the early-upregulated LPMO enzyme to work in association with CMF chemistry, suggesting that G. trabeum may have adopted mechanisms to integrate non-enzymatic and enzymatic chemistries together during early stages of brown rot decay.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2016

BdCESA7 , BdCESA8 , and BdPMT utility promoter constructs for targeted expression to secondary cell-wall-forming cells of grasses

Utility vectors with promoters that confer desired spatial and temporal expression patterns are useful tools for studying gene and cellular function and for industrial applications. To target the expression of DNA sequences of interest to cells forming plant secondary cell walls, which generate most of the vegetative biomass, upstream regulatory sequences of the Brachypodium distachyon lignin biosynthetic gene BdPMT and the cellulose synthase genes BdCESA7 and BdCESA8 were isolated and cloned into binary vectors designed for Agrobacterium -mediated transformation of monocots. Expression patterns were assessed using the β-glucuronidase gene GUSPlus and X-glucuronide staining. All three promoters showed strong expression levels in stem tissue at the base of internodes where cell wall deposition is most active, in both vascular bundle xylem vessels and tracheids, and in interfascicular tissues, with expression less pronounced in developmentally older tissues. In leaves, BdCESA7 and BdCESA8 promoter-driven expression was strongest in leaf veins, leaf margins, and trichomes; relatively weaker and patchy expression was observed in the epidermis. BdPMT promoter-driven expression was similar to the BdCESA promoters expression patterns, including strong expression in trichomes. The intensity and extent of GUS staining varied considerably between transgenic lines, suggesting that positional effects influenced promoter activity. Introducing the BdPMT and BdCESA8 Open Reading Frames into BdPMT and BdCESA8 utility promoter binary vectors, respectively, and transforming those constructs into Brachypodium pmt and cesa8 loss-of-function mutants resulted in rescue of the corresponding mutant phenotypes. This work therefore validates the functionality of these utility promoter binary vectors for use in Brachypodium and likely other grass species. Lastly, the identification, in Bdcesa8-1 T-DNA mutant stems, of an 80% reduction in crystalline cellulose levels confirms that the BdCESA8 gene is a secondary-cell-wall-forming cellulose synthase.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

Visualization of lignification in flax stem cell walls with novel click-compatible monolignol analogs

As an essential part of plant cell walls, lignin provides mechanical support for plant growth, enhances water transport, and helps to defend against pathogens. As the most abundant natural aromatic-based renewable resource on earth, its biosynthesis has always been a research focus, and it is still currently under study. In this study, the p-coumaryl alcohol analog (H ALK ) and the coniferyl alcohol analog (G ALK ) containing an alkyne group at the ortho position were synthesized and applied to lignification in vivo and in vitro. The incorporation of these novel lignin monomers was observed via fluorescence imaging. It was found that the two monolignol analogs could be incorporated in dehydrogenated polymers (DHPs) in vitro and in flax cell walls in vivo. The results showed that as the cultivation time and precursor concentration varied, the deposition of H and G-type lignin exhibited differences in deposition mode. At the subcellular scale, the deposited lignin first appears in the cell corner and the middle lamella, and then gradually appears on the cell walls. Furthermore, lignin was also found in bast fiber. It was demonstrated that these new molecules could provide high-resolution localization of lignin during polymerization.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Particle Matter Index and Fuel Wall-wetting Relations on Stochastic Pre-ignition

This works explores the effect of the particle matter index (PMI) and aromatic content on fuel wall impingement and associated stochastic pre-ignition (SPI) propensity. Statically significant measurements of SPI rates are directly coupled with laser induced florescence (LIF) measurements of fuel dilution from spray-linear impingement. Literature suggests that PMI is could be correlated with the number of SPI events, but the root cause(s) of PMI and SPI are directly causational or are a predicator of SPI. Three fuels have been used in this study with 3 different PMI and two different aromatic contents. These fuels are direct injected at two different injection timings, an earlier injection timing which targets the piston crown, 310°CA bTDC, and a later injection timing that the linear, 220°CA bTDC start of injection timings (SOI) respectively. The earlier 310 SOI injection increases soot, whereas the later 220°CD SOI targets the linear and increases wall-wetting. The findings of this work highlight that the SPI activity has a weak correlation with the soot promoted by the combustion process and increased PMI. However, SPI activity shows a strong dependency with high levels of oil dilution and aromatic content, suggesting that PMI might be an indicator of fuel chemistry conducive to SPI and the soot form increased PMI fuels is not a strong source of SPI relative to fuel wall wetting.

42 ENGINEERING↗

NASA NTRS1969

Nondestructive testing of welds on thin-walled tubing

Special ultrasonic search unit, or transducer assembly, reliably inspects the quality of melt-through welds of fusion welded tubing couplers for hydraulic lines. This instrumentation can also be used to detect faulty braze bonds in thin-walled, small diameter joints and wall thickness of thin-walled metal tubing.

Hagemaier, D. J.↗

NASA NTRS1972

Halogenation of microcapsule walls

Procedure for halogenation of confining walls of both gelatin and gelatin-phenolic resin capsules is similar to that used for microencapsulation. Ten percent halogen content renders capsule wall nonburning; any higher content enhances flame-retardant properties of selected internal phase material. Halogenation decreases permeability of wall material to encapsulated materials.

Davis, T. R.↗

NASA NTRS1971

Nozzle and cavity wall cooling limitations on specific impulse of a gas-core nuclear rocket.

Experimental and theoretical study of the performance of a gas-core nuclear rocket, showing that in a uranium plasma nuclear rocket with an 8-ft cavity diameter the cavity wall can be cooled up to a power level of 7400 MW when the rocket operates at a pressure of 1000 atm and the propellant mass flow rate is 10 lbm/sec. A maximum cavity specific impulse of 5800 sec was obtained under such operating conditions. The fact that the wall heat flux was much lower for reactor powers below this level is linked to the presence of a relatively cool, opaque insulating layer of seeded propellant between the hot plasma and the solid wall. The additional coolant required for nozzle protection reduced the maximum cavity specific impulse to 5200 sec.

Kascak, A. F.↗

NASA NTRS1972

Air film cooling in a nonadiabatic wall conical nozzle.

Experimental data for an air-film cooled conical nozzle operating with a heated-air main stream and a water-cooled wall confirm the validity of Lieu's (1964) method for correlating film cooling data in the accelerated flow of a nonadiabatic-wall nozzle. The film cooling effectiveness modified for nonadiabatic walls by Lieu can be used to correlate film cooling under the condition that the main-stream to coolant velocity ratio at the slot is about 1. Such a ratio provides the optimum cooling effectiveness.

Boldman, D. R.↗

NASA NTRS1972

Computation of wall effects in ventilated transonic wind tunnels.

A method for computing interference effects due to ventilated wind-tunnel walls on lifting transonic airfoils is presented. Differences between the present method based on nonlinear transonic theory and the classical correction formulae from linear subsonic theory are discussed. Computed results are presented for an NACA 0012 in solid and perforated wall tunnels. The solid wall calculations are compared with data.

Murman, E. M.↗

NASA NTRS1973

Control of exit velocity profile of an asymmetric annular diffuser using wall suction

An asymmetric annular diffuser equipped with wall bleed (suction) capability was tested for controllability of exit velocity profile. The diffuser area ratio was 3.2, and the length to inlet height ratio was 1.6. Results show that the diffuser radial exit velocity profile could be controlled from a hub peaked to a tip peaked form by selective use of bleed on the outer wall or on both diffuser walls. Based on these results, application of the diffuser bleed technique to gas turbine combustors may be possible. Diffuser bleed could be used to tailor the airflow distribution for optimizing combustor performance at a variety of operating conditions.

Juhasz, A. J.↗

NASA NTRS1973

Design and experimental performance of short curved wall diffusers with axial symmetry utilizing slot suction

The feasibility of designing short curved wall axially symmetrical subsonic diffusers utilizing suction through slots in the diffuser walls to prevent flow separation was investigated. A potential flow analysis was made, and a digital computer program was written for determining the diffuser wall contour for prescribed boundary conditions. The flow field included branch flow so that the suction slot geometry could be a part of the diffuser design. One bell shaped diffuser and three annular diffusers with area ratios of either 2.5:1 or 3:1 were designed, fabricated, and tested. Minimum suction requirements of metastable operation ranged from 6.3 percent to 12 percent when operating with inlet air velocities in the 1000 to 250 ft/sec (30 to 76 m/sec) range. For stable operation suction rates from 10 percent to 22 percent were required. In all cases the diffuser effectiveness was above 95 percent based on the conventional definition, and from 81 percent to 94 percent when the suction loss was accounted for. The exit velocity profiles were virtually flat with no more than + or - 9% variation over 95 percent of the exit area when operated with sufficient suction to prevent flow separation.

Yang, T.↗

NASA NTRS1973

Net radiation method for enclosure systems involving partially transparent walls

The net radiation method is developed for analyzing radiation heat transfer in enclosure systems involving partially transparent walls. One such system is an enclosure with windows in it. The conventional net radiation method was developed for enclosures having opaque walls. If a partially transparent wall is present, it will permit radiation to enter and leave the enclosure. The net radiation equations are developed here for gray and semigray enclosures with one or more windows. Another system of interest, such as in a flat plate solar collector, consists of a series of parallel transparent layers. The transmission characteristics of such window systems are obtained by the net radiation method, and the technique appears to be more convenient than the ray tracing method which has been used in the past. Relations are developed for windows consisting of any number of parallel layers having differing absorption coefficients and differing surface reflectivities, and for systems composed of parallel transmitting layers and opaque plates.

Siegel, R.↗

NASA NTRS1973

Experimental investigation of a wall stablized arc with comparisons to theoretical predictions

A wall-stabilized, constricted-arc heater was investigated by extensive measurements of pressure, voltage, and wall heat flux at different axial locations along the constrictor. Radial temperature profiles were measured at one axial location by the radiance of a single line method and also by the continuum radiance at a wavelength of 4955 A. The arc heater with an inside diameter of 2.37 cm and a constrictor length of 42 cm was operated with nitrogen flow rate from about 2 to 10 g/sec, and are currents from 400 to 1200 amperes. Based on a literature search, two different digital computer programs were selected which were used to predict the pertinent parameters by numerical solutions to the governing differential equations. The program selections were based on completeness and availability. In general the predictions were adequate when laminar flow was assumed, and the greatest difficulty was encountered in the predictions of wall heat flux. The results of the measurements are shown in graphical form, along with the theoretical predictions from both computer programs.

Wells, W. L.↗

NASA NTRS1973

Experimental study of shock-wave reflection from a thermally accommodating wall.

Shock-tube experiments have been conducted to study the nonequilibrium gas-surface interaction which occurs when a thick shock wave in argon reflects from a coplanar, heat-conducting wall. Fast-response instrumentation was used to monitor variations in temperature and normal stress on the surface of the shock-tube end wall during and immediately following reflection of the incident shock wave. The laboratory observations are compared with computer predictions obtained by Deiwert using the direct-simulation Monte Carlo method, and excellent agreement is obtained when a suitable average thermal accommodation coefficient is chosen for the wall surface.

Hanson, R. K.↗

NASA NTRS1973

Reflection of a shock wave from a thermally accommodating wall - Molecular simulation.

Reflection of a plane shock wave from a wall has been simulated on a microscopic scale using a direct simulation Monte Carlo technique of the type developed by Bird. A monatomic gas model representing argon was used to describe the fluid medium and a simple one-parameter accommodation coefficient model was used to describe the gas-surface interaction. The influence of surface accommodation was studied parametrically by varying the accommodation coefficient from zero to one. Results are presented showing the temporal variations of flow field density, and mass, momentum, and energy fluxes to the wall during the shock wave reflection process. The energy flux was used to determine the wall temperature history. Comparisons with experiment are found to be satisfactory where data are available.

Deiwert, G. S.↗

NASA NTRS1974

Discrete wall jets in quiescent air

An experimental investigation was made of turbulent jet flows resulting from small, round nozzles discharging parallel to a smooth, flat wall in quiescent air. Nozzle axes were located 3.0 nozzle diameters above the wall surface. The case of a single nozzle and the case of a spanwise array of equally spaced nozzles were investigated. Several forms of approximate velocity profile similarity were noted, and the flow from the array of nozzles was seen to approach the form of a two-dimensional wall jet.

Mclean, J. D.↗

NASA NTRS1974

Evaluation of analytical procedures for prediction of turbulent boundary layers on a porous wall

An analytical study has been made to determine how well current boundary layer prediction techniques work when there is mass transfer normal to the wall. The data that were considered in this investigation were for two-dimensional, incompressible, turbulent boundary layers with suction and blowing. Some of the bleed data were taken in an adverse pressure gradient. An integral prediction method was used three different porous wall skin friction relations, in addition to a solid-surface relation for the suction cases. A numerical prediction method was also used. Comparisons were made between theoretical and experimental skin friction coefficients, displacement and momentum thicknesses, and velocity profiles. The integral method with one of the porous wall skin friction laws gave very good agreement with data for most of the cases considered. The use of the solid-surface skin friction law caused the integral to overpredict the effectiveness of the bleed. The numerical techniques also worked well for most of the cases.

Towne, C. E.↗

NASA NTRS1974

Refractive index of plant cell walls

Air was replaced with media of higher refractive indices by vacuum infiltration in leaves of cucumber, blackeye pea, tomato, and string bean plants, and reflectance of noninfiltrated and infiltrated leaves was spectrophotometrically measured. Infiltrated leaves reflected less light than noninfiltrated leaves over the 500-2500-nm wavelength interval because cell wall-air interfaces were partly eliminated. Minimal reflectance should occur when the average refractive index of plant cell walls was matched by the infiltrating fluid. Although refractive indices that resulted in minimal reflectance differed among the four plant genera, an average value of 1.425 approximates the refractive index of plant cell walls for the four plant genera.

Gausman, H. W.↗