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At least 1,315 records · Page 73

Evaluation of Carburized and Ground Face Gears

Experimental durability tests were performed on carburized and ground AIS19310 steel face gears. The tests were in support of a Defense Advanced Research Projects Agency (DARPA) Technology Reinvestment Program (TRP) to enhance face-gear technology. The tests were conducted in the NASA Glenn spiral-bevel-gear/face-gear test facility. Tests were run at 2300 rpm face gear speed and at loads of 64, 76, 88, 100, and 112-percent of the design torque of 377 N-m (3340 in-lb). The carburized and ground face gears demonstrated the required durability when run for ten-million cycles at each of the applied loads. Proper installation was critical for the successful operation of the spur pinions and face gears. A large amount of backlash produced tooth contact patterns that approached the inner-diameter edge of the face-gear tooth. Low backlash produced tooth contact patterns that approached the outer-diameter edge of the face-gear tooth. Measured backlashes in the range of 0.178 to 0.254 mm (0.007 to 0.010 in) produced acceptable tooth contact patterns.

Lewicki, David G.↗

Recent Development in the Analytical Investigation of Shipboard Rotorcraft Engage and Disengage Operations

This paper presents an overview of recent developments in an effort to predict transient aeroelastic rotor response during shipboard engage and disengage sequences. The blade is modeled as an elastic beam undergoing in flap, lag, extension and torsion. The blade equations of motion are formulated using Hamilton's principle and they are spatially discretized using the finite element method. The discretized blade equations of motion are integrated for a specified rotor speed run-up or run-down profile. Blade element theory is used to calculate quasi-steady or unsteady aerodynamic loads in linear and nonlinear regimes. The analysis is capable of simulating both articulated, hingeless, and gimballed rotor systems. Validation of the rotor code is discussed, including correlation with droop stop impact tests and wind tunnel experiments. Predictions of safe engagement and disengagement envelopes, limited by excessive blade tip deflections or hub moments, are presented. Future directions of study are also discussed.

Smith, Edward C.↗

The Development of Kolmogoroff-Like Power Spectra in the Expanding Solar Wind

Power spectra of the solar wind fluctuations consistently exhibit a -5/3 power-law slope consistent with the idea that the medium is undergoing a turbulent cascade as seen in ordinary fluids. This is surprising both because the radial streams and the magnetic field threading the plasma will induce anisotropies and because the expansion of the wind will tend to lead to the suppression of nonlinear cascades. These conditions violate the assumptions used by Kolmogoroff to derive the -5/3 law. We have studied this issue using a compressible IMHD code in spherical coordinates and have shown that a -5/3 spectrum results from a broad-band flat-spectrum input condition that is sheared and distorted by a current sheet as the wind expands. We determine spectra from time series taken at selected points in the domain as is done with observational spacecraft data. The spectra are very like those we have seen in nonexpanding runs and exhibit evolution and compressive characteristics very similar to those seen in observations. We will report on these results in addition to a new set of runs intended to constrain the necessary and sufficient conditions for the spectra to have this form. The simulation also allows us to examine the anisotropy for the spectra to attempt to determine why the result of an isotropic magnetofluid is obtained in a highly anisotropic situation.

Goldstein, M. L.↗

Research on Spectroscopy, Opacity, and Atmospheres

A web site has been set up to make the calculations accessible; (i.e., cfakus.harvard.edu) This data can also be accessed by FTP. It has all of the atomic and diatomic molecular data, tables of distribution function opacities, grids of model atmospheres, colors, fluxes, etc, programs that are ready for distribution, and most of recent papers developed during this grant. Atlases and computed spectra will be added as they are completed. New atomic and molecular calculations will be added as they are completed. The atomic programs that had been running on a Cray at the San Diego Supercomputer Center can now run on the Vaxes and Alpha. The work started with Ni and Co because there were new laboratory analyses that included isotopic and hyperfine splitting. Those calculations are described in the appended abstract for the 6th Atomic Spectroscopy and oscillator Strengths meeting in Victoria last summer. A surprising finding is that quadrupole transitions have been grossly in error because mixing with higher levels has not been included. All levels up through n=9 for Fe I and II, the spectra for which the most information is available, are now included. After Fe I and Fe II, all other spectra are "easy". ATLAS12, the opacity sampling program for computing models with arbitrary abundances, has been put on the web server. A new distribution function opacity program for workstations that replaces the one used on the Cray at the San Diego Supercomputer Center has been written. Each set of abundances would take 100 Cray hours costing $100,000.

Kurucz, Robert L.↗

To the Die Smartly: Heavy Ion Testing of PEMs on COTS Boards Through the Plastic

Seven commercial off the shelf (COTS) boards containing electronic devices (all in plastic packages, PEMS), under consideration for use in a spacecraft subsystem, were exposed to beams of very high energy ions at the National Superconducting Cyclotron Laboratory (MSU). The ion energies were high enough that an entire board could be exposed in air, and it could still penetrate through the plastic and reach the silicon die. A total of about 300 runs were made, and for each, the LET of the ion entering the silicon die had to be determined, based on the thickness of the plastic lid and the thickness of overlaying materials (e.g., aluminum degraders). Single event latchup (SEL) and functional interrupt (SEFI) were determined during each run, the SEFI by means of simple programs being continuously written to and read from the boards to monitor functionality, while each part was being exposed to the heavy ions.

Wert, J. L.↗

Simulation of 1986 South China Sea Monsoon with a Regional Climate Model

A Regional Land-Atmosphere Climate Simulation System (RELACS) project is being developed at NASA Goddard Space Flight Center. One of the major goals of RELACS is to use a regional scale model with improved physical processes and in particular land-related processes, to understand the role of the land surface and its interaction with convection and radiation as well as the water/energy cycles in the IndoChina/South China Sea (SCS) region. The Penn State/NCAR MM5 atmospheric modeling system, a state of the art atmospheric numerical model designed to simulate regional weather and climate, has been successfully coupled to the Parameterization for Land-Atmosphere-Cloud Exchange (PLACE) land surface model. The original MM5 model (without PLACE) includes the option for either a simple slab soil model or a five-layer soil model (MRF) in which the soil moisture availability evolves over time. However, the MM5 soil models do not include the effects of vegetation, and thus important physical processes such as evapotranspiration and interception are precluded. The PLACE model incorporates vegetation type and has been shown in international comparisons to accurately predict evapotranspiration and runoff over a wide variety of land surfaces. The coupling of MM5 and PLACE creates a numerical modeling system with the potential to more realistically simulate atmosphere and land surface processes including land-sea interaction, regional circulations such as monsoons, and flash flood events. In addition, the Penn State/NCAR MM5 atmospheric modeling system has been: (1) coupled to the Goddard Ice Microphysical scheme; (2) coupled to a turbulent kinetic energy (TKE) scheme; (3) modified to ensure cloud budget balance; and (4) incorporated initialization with the Goddard EOS data sets at NASA/Goddard Laboratory for Atmospheres. The improved MM5 with two nested domains (60 and 20 km horizontal resolution) was used to simulate convective activity over IndoChina and the South China Sea, during the monsoon season, from May 6 to May 20, 1986. The model results captured several dominant observed features, such as twin cyclones, a depression system over the Bay of Bengal, strong south-westerly winds over IndoChina before and during the on-set of convection over the SCS, and a vortex over the SCS. Two additional MM5 runs with different land process models, Blackadar and MRF, were performed, and their results are compared to the run with PLACE. The preliminary results indicate that the MM5 results using PLACE and Blackadar are in very good agreement, but the results using MRF do not contain the south-westerly wind over IndoChina prior to the on-set of convection over the SCS.

Tao, W. -K.↗

Local Data Integration in East Central Florida

The Applied Meteorology Unit has configured a Local Data Integration System (LDIS) for east central Florida which assimilates in-situ and remotely-sensed observational data into a series of high-resolution gridded analyses. The ultimate goal for running LDIS is to generate products that may enhance weather nowcasts and short-range (less than 6 h) forecasts issued in support of the 45th Weather Squadron (45 WS), Spaceflight Meteorology Group (SMG), and the Melbourne National Weather Service (NWS MLB) operational requirements. LDIS has the potential to provide added value for nowcasts and short-ten-n forecasts for two reasons. First, it incorporates all data operationally available in east central Florida. Second, it is run at finer spatial and temporal resolutions than current national-scale operational models such as the Rapid Update Cycle and Eta models. LDIS combines all available data to produce grid analyses of primary variables (wind, temperature, etc.) at specified temporal and spatial resolutions. These analyses of primary variables can be used to compute diagnostic quantities such as vorticity and divergence. This paper demonstrates the utility of LDIS over east central Florida for a warm season case study. The evolution of a significant thunderstorm outflow boundary is depicted through horizontal and vertical cross section plots of wind speed, divergence, and circulation. In combination with a suitable visualization too], LDIS may provide users with a more complete and comprehensive understanding of evolving mesoscale weather than could be developed by individually examining the disparate data sets over the same area and time.

Case, Jonathan L.↗

Aerosols and Particulates Workshop Sampling Procedures and Venues Working Group Summary

The Sampling Procedures and Venues Workgroup discussed the potential venues available and issues associated with obtaining measurements. Some of the issues included Incoming Air Quality, Sampling Locations, Probes and Sample Systems. The following is a summary of the discussion of the issues and venues. The influence of inlet air to the measurement of exhaust species, especially trace chemical species, must be considered. Analysis procedures for current engine exhaust emissions regulatory measurements require adjustments for air inlet humidity. As a matter of course in scientific investigations, it is recommended that "background" measurements for any species, particulate or chemical, be performed during inlet air flow before initiation of combustion, if possible, and during the engine test period as feasible and practical. For current regulatory measurements, this would be equivalent to setting the "zero" level for conventional gas analyzers. As a minimum, it is recommended that measurements of the humidity and particulates in the incoming air be taken at the start and end of each test run. Additional measurement points taken during the run are desirable if they can be practically obtained. It was felt that the presence of trace gases in the incoming air is not a significant problem. However, investigators should consider the ambient levels and influences of local air pollution for species of interest. Desired measurement locations depend upon the investigation requirements. A complete investigation of phenomenology of particulate formation and growth requires measurements at a number of locations both within the engine and in the exhaust field downstream of the nozzle exit plane. Desirable locations for both extractive and in situ measurements include: (1) Combustion Zone (Multiple axial locations); (2) Combustor Exit (Multiple radial locations for annular combustors); (3) Turbine Stage (Inlet and exit of the stage); (4) Exit Nozzle (Multiple axial locations downstream of the nozzle). Actual locations with potential for extractive or non-intrusive measurements depend upon the test article and test configuration. Committee members expressed the importance of making investigators aware of various ports that could allow access to various stages of the existing engines. Port locations are engine si)ecific and might allow extractive sampling or innovative hybrid optical-probe access. The turbine stage region was one the most desirable locations for obtaining samples and might be accessed through boroscope ports available in some engine designs. Discussions of probes and sampling systems quickly identified issues dependent on particular measurement quantities. With general consensus, the group recommends SAE procedures for measurements and data analyses of currently regulated exhaust species (CO2, CO, THC, NO(x),) using conventional gas sampling techniques. Special procedures following sound scientific practices must be developed as required for species and/or measurement conditions not covered by SAE standards. Several issues arose concerning short lived radicals and highly reactive species. For conventional sampling, there are concerns of perturbing the sample during extraction, line losses, line-wall reactions, and chemical reactions during the sample transport to the analyzers. Sample lines coated with quartz.or other materials should be investigated for minimization of such effects. The group advocates the development of innovative probe techniques and non-intrusive optical techniques for measurement of short lived radicals and highly reactive species that cannot be sampled accurately otherwise. Two innovative probe concepts were discussed. One concept uses specially designed probes to transfer optical beams to and from a region of flow inaccessible by traditional ports or windows. The probe can perturb the flow field but must have a negligible impact on the region to be optically sampled. Such probes are referred to as hybrid probes and are under development at AEDC for measurement in the high pressure, high temperature of a combustor under development for power generation. The other concept consists of coupling an instrument directly to the probe. The probe would isolate a representative sample stream, freeze chemical reactions and direct the sample into the analyzer portion of the probe. Thus, the measurement would be performed in situ without sample line losses due either to reactions or binding at the wall surfaces. This concept was used to develop a fast, in situ, time-of-flight mass spectrometer measurement system for temporal quantification of NO in the IMPULSE facility at AEDC. Additional work is required in this area to determine the best probe and sampling technique for each species measurement requirement identified by the Trace Chemistry Working Group. A partial list of Venues was used as a baseline for discussion. Additional venues were added to the list and the list was broken out into the following categories: (1)Engines (a) Sea Level Test Stands (b) Altitude Chambers; (2) Annular Combustor Test Stands, (3) Sector Flametube Test Stands, (4) Fundamentals Rigs/Experiments.

Pachlhofer, Peter↗

Current and Future Plans of the NASA Data Assimilation Office (DAO)

The mission of the Data Assimilation Office (DAO) is to advance the state of the art of data assimilation and produce research-quality assimilated data sets which make optimal use of space-based observations. Development efforts over the last few years have focused on delivering a production data assimilation system in support of NASA's Terra platform. That system, called the Goddard Earth Observing System - version 2 or GEOS-2, represents a major upgrade to the baseline GEOS-1 system employed in NASA's first reanalysis effort. GEOS-2 includes a physical-space three dimensional variational analysis algorithm (the Physical-space Statistical Analysis System or PSAS) and numerous improvements to the general circulation model. The latter include a Soil-Vegetation-Atmosphere Transfer (SVAT) land surface scheme, a level 2.5 moist turbulence scheme and new Short Wave (SW) and Long Wave (LW) radiation code. The system also includes an off-line ozone assimilation system, and the capability to assimilate scatterometer surface winds, and TIROS Operational Vertical Sounder (TOVS) and Special Sensor Microwave Imager (SSM/I) moisture data. GEOS-2 is currently run at 1 degree horizontal resolution and 48 levels extending to O.Olmb. Experimental versions of GEOS-2 are run with a global stretched grid allowing enhanced (e.g. 1/4 deg) regional resolution. Other capabilities being developed include, the assimilation of Tropical Rainfall Measuring Mission (TRMM) precipitation and Global Positioning System (GPS) data, an off-line land surface assimilation system, and a retrospective analysis scheme. The DAO is also engaged in a number of collaborative efforts to help accelerate the development of the next generation data assimilation system. These include, a joint modeling effort between the DAO and NCAR/CGDD to develop a new Global Circulation Model (GCM), and a Department of Energy Lawrence Livermore National Laboratory (DOE/LLNL) collaboration on model parallelization. Plans for the next reanalysis will be discussed in the context of current and near term system quality and computing capabilities, and the need for multiple reanalysis products.

Atlas, Robert↗

Assimilation of Geosat Altimetric Data in a Nonlinear Shallow-Water Model of the Indian Ocean by Adjoint Approach. Part II: Some Validation and Interpretation of the Assimilated Results: Some Validation and Interpretation of the Assimilated Results - Part 2

This paper examines the results of assimilating Geosat sea level variations relative to the November 1986-November 1988 mean reference, in a nonlinear reduced-gravity model of the Indian Ocean, Data have been assimilated during one year starting in November 1986 with the objective of optimizing the initial conditions and the yearly averaged reference surface. The thermocline slope simulated by the model with or without assimilation is validated by comparison with the signal, which can be derived from expandable bathythermograph measurements performed in the Indian Ocean at that time. The topography simulated with assimilation on November 1986 is in very good agreement with the hydrographic data. The slopes corresponding to the South Equatorial Current and to the South Equatorial Countercurrent are better reproduced with assimilation than without during the first nine months. The whole circulation of the cyclonic gyre south of the equator is then strongly intensified by assimilation. Another assimilation experiment is run over the following year starting in November 1987. The difference between the two yearly mean surfaces simulated with assimilation is in excellent agreement with Geosat. In the southeastern Indian Ocean, the correction to the yearly mean dynamic topography due to assimilation over the second year is negatively correlated to the one the year before. This correction is also in agreement with hydrographic data. It is likely that the signal corrected by assimilation is not only due to wind error, because simulations driven by various wind forcings present the same features over the two years. Model simulations run with a prescribed throughflow transport anomaly indicate that assimilation is rather correcting in the interior of the model domain for inadequate boundary conditions with the Pacific.

Greiner, Eric↗

Research on Spectroscopy, Opacity, and Atmospheres

To make my calculations more readily accessible I have set up a web site cfaku5.harvard.edu that can also be accessed by FTP. it has 5 9GB disks that hold all of my atomic and diatomic molecular data, my tables of distribution function opacities, my grids of model atmospheres, colors, fluxes, etc, my program that are ready for distribution, most of my recent papers. Atlases and computed spectra will be added as they are completed. New atomic and molecular calculations will be added as they are completed. I got my atomic programs that had been running on a Cray at the San Diego Supercomputer Center to run on my Vaxes and Alpha. I started with Ni and Co because there were new laboratory analyses that included isotopic and hyperfine splitting. Those calculations are described in the appended abstract for the 6th Atomic Spectroscopy and oscillator Strengths meeting in Victoria last summer. A surprising finding is that quadrupole transitions have been grossly in error because mixing with higher levels has not been included. I now have enough memory in my Alpha to treat 3000 x 3000 matrices. I now include all levels up through n=9 for Fe I and 11, the spectra for which the most information is available. I am finishing those calculations right now. After Fe I and Fe 11, all other spectra are "easy", and I will be in mass production. ATL;LS12, my opacity sampling program for computing models with arbitrary abundances, has been put on the web server. I wrote a new distribution function opacity program for workstations that replaces the one I used on the Cray at the San Diego Supercomputer Center. Each set of abundances would take 100 Cray hours costing $100,000. 1 ran 25 cases. Each of my opacity CDs contains three abundances. I have a new program -iinning on the Alpha that takes about a week. I am going to have to get a faster processor or I will have to dedicate a whole workstation just to opacities.

Kurucz, Robert L.↗

Application of the RNS3D Code to a Circular-Rectangular Transition Duct With and Without Inlet Swirl and Comparison with Experiments

Circular-rectangular transition ducts are used between engine exhausts and nozzles with rectangular cross sections that are designed for high performance aircraft. NASA Glenn Research Center has made experimental investigations of a series of circular-rectangular transition ducts to provide benchmark flow data for comparison with numerical calculations. These ducts are all designed with superellipse cross sections to facilitate grid generation. In response to this challenge, the three-dimensional RNS3D code has been applied to one of these transition ducts. This particular duct has a length-to-inlet diameter ratio of 1.5 and an exit-plane aspect ratio of 3.0. The inlet Mach number is 0.35. Two GRC experiments and the code were run for this duct without inlet swirl. One GRC experiment and the code were also run with inlet swirl. With no inlet swirl the code was successful in predicting pressures and secondary flow conditions, including a pair of counter-rotating vortices at both sidewalls of the exit plane. All these phenomena have been reported from the two GRC experiments. However, these vortices were suppressed in the one experiment when inlet swirl was used; whereas the RNS3D code still predicted them. The experiment was unable to provide data near the sidewalls, the very region where the vortices were predicted.

Cavicchi, Richard H.↗

Characterization of In-Situ BN Interface Formed by Nitridation of Nextel 312

Treatment of Nextel (TM) 312 in a reactive environment has been used as a low cost approach to the formation of BN on the fiber surface. Although the BN enriched surface region is extremely thin (less than 40 run), it is effective in providing debonding and composite behavior. Variation in mechanical behavior of Nextel (TM) 312/BN/Blackglas (TM) composites has been noted among panels fabricated with AF-10 cloth treated in different nitridation runs, Understanding this variation became the rationale for undertaking a detailed characterization of the nitrided fiber using field emission scanning electron microscopy, low voltage energy dispersive x-ray and Auger electron spectroscopy. Chemical composition and surface morphology are discussed in relation to observed mechanical behavior of NeXtel (TM) 312/Blackglas (TM) composites tested in bending.

Hurwitz, Fances I.↗

Improvements to the Unstructured Mesh Generator MESH3D

The AIRPLANE process starts with an aircraft geometry stored in a CAD system. The surface is modeled with a mesh of triangles and then the flow solver produces pressures at surface points which may be integrated to find forces and moments. The biggest advantage is that the grid generation bottleneck of the CFD process is eliminated when an unstructured tetrahedral mesh is used. MESH3D is the key to turning around the first analysis of a CAD geometry in days instead of weeks. The flow solver part of AIRPLANE has proven to be robust and accurate over a decade of use at NASA. It has been extensively validated with experimental data and compares well with other Euler flow solvers. AIRPLANE has been applied to all the HSR geometries treated at Ames over the course of the HSR program in order to verify the accuracy of other flow solvers. The unstructured approach makes handling complete and complex geometries very simple because only the surface of the aircraft needs to be discretized, i.e. covered with triangles. The volume mesh is created automatically by MESH3D. AIRPLANE runs well on multiple platforms. Vectorization on the Cray Y-MP is reasonable for a code that uses indirect addressing. Massively parallel computers such as the IBM SP2, SGI Origin 2000, and the Cray T3E have been used with an MPI version of the flow solver and the code scales very well on these systems. AIRPLANE can run on a desktop computer as well. AIRPLANE has a future. The unstructured technologies developed as part of the HSR program are now targeting high Reynolds number viscous flow simulation. The pacing item in this effort is Navier-Stokes mesh generation.

Thomas, Scott D.↗

Very High Current Density Nb/AlN/Nb Tunnel Junctions for Low-Noise Submillimeter Mixers

We have fabricated and tested submillimeter-wave superconductor-insulator-superconductor (SIS) mixers using very high current density Nb/AlN/Nb tunnel junctions (J(sub c) approximately equal 30 kA/sq cm) . The junctions have low resistance-area products (R(sub N)A approximately 5.6 Omega.sq micron), good subgap to normal resistance ratios R(sub sg)/R(sub N) approximately equal 10, and good run-to-run reproducibility. From Fourier transform spectrometer measurements, we infer that omega.R(sub N)C = 1 at 270 GHz. This is a factor of 2.5 improvement over what is generally available with Nb/AlO(x)/Nb junctions suitable for low-noise mixers. The AlN-barrier junctions are indeed capable of low-noise operation: we measure an uncorrected receiver noise temperature of T(sub RX) = 110 K (DSB) at 533 GHz for an unoptimized device. In addition to providing wider bandwidth operation at lower frequencies, the AlN-barrier junctions will considerably improve the performance of THz SIS mixers by reducing RF loss in the tuning circuits.

Kawamura, Jonathan↗

Examination of Soil Moisture Retrieval Using SIR-C Radar Data and a Distributed Hydrological Model

A major objective of soil moisture-related hydrological-research during NASA's SIR-C/X-SAR mission was to determine and compare soil moisture patterns within humid watersheds using SAR data, ground-based measurements, and hydrologic modeling. Currently available soil moisture-inversion methods using active microwave data are only accurate when applied to bare and slightly vegetated surfaces. Moreover, as the surface dries down, the number of pixels that can provide estimated soil moisture by these radar inversion methods decreases, leading to less accuracy and, confidence in the retrieved soil moisture fields at the watershed scale. The impact of these errors in microwave- derived soil moisture on hydrological modeling of vegetated watersheds has yet to be addressed. In this study a coupled water and energy balance model operating within a topographic framework is used to predict surface soil moisture for both bare and vegetated areas. In the first model run, the hydrological model is initialized using a standard baseflow approach, while in the second model run, soil moisture values derived from SIR-C radar data are used for initialization. The results, which compare favorably with ground measurements, demonstrate the utility of combining radar-derived surface soil moisture information with basin-scale hydrological modeling.

Hsu, A. Y.↗

Size Exclusion Chromatography Studies of the Initial Self-Association Steps of Chicken Egg White Lysozyme Nucleation

Nucleation is one of the least understood aspects of crystallogenesis. In the case of macromolecule nucleation, this understanding is further hampered by uncertainty over what precisely is being discussed. We define the process of solute self-association (aggregation, oligomerization, interaction, clustering, etc.) whereby n-mers (n > or = 2) having a crystallographic or nascent crystallographic arrangement leading to the critical nucleus reversibly form in the solution, to be part of the nucleation process. This reversible self-association process is a fundamental part of the nucleation process, and occurs as a function of the solute concentration. In the case of chicken egg white lysozyme, a considerable body of experimental evidence leads us to the conclusion that it also forms the crystal growth units. Size exclusion chromatography is a simple and direct method for determining the equilibrium constants for the self-association process. A Pharmacia FPLC system was used to provide accurate solution flow rates. The column, injection valve, and sample loop were all mounted within a temperature-controlled chamber. Chromatographically re-purified lysozyme was first dialyzed against the column equilibration buffer, with injection onto the column after several hours pre-incubation at the running temperature. Preliminary experiments, were carried out using a Toyopearl HW-50F column (1 x 50cm), equilibrated with 0.1 M sodium acetate, 5% sodium chloride, pH 4.6, at 15C. Protein concentrations from 0.1 to 4 mg/ml were employed (C(sub sat) = 1.2 mg/ml). The data from several different protein preparations consistently shows a progressively decreasing elution volume with increasing protein concentration, indicating that reversible self-association is occurring. The dotted line indicates the monomeric lysozyme elution volume. However, lysozyme interacts with the column matrix in these experiments, which complicates data analysis.Accordingly, we are testing silica-based HPLC columns in an effort to eliminate this problem and substantially reduce the column volume and experimental run time. The results and data analysis from these and subsequent experiments will be presented.

Ewing, Felecia↗

Ground Reaction Forces During Locomotion in Simulated Microgravity

Significant losses in bone density and mineral, primarily in the lower extremities have been reported following exposure to weightlessness. Recent investigations suggest that mechanical influences such as bone deformation and strain rate may be critically important in stimulating new bone formation. It was hypothesized that velocity, cadence and harness design would significantly affect lower limb impact forces during treadmill exercise in simulated zero gravity (0G). A ground-based hypogravity simulator was used to investigate which factors affect limb loading during tethered treadmill exercise. A fractional factorial design was used and 12 subjects were studied. The results showed that running on active and passive treadmills in the simulator with a tethering force close to the maximum comfortable level produced similar magnitudes for the peak ground reaction force. It was also found that these maximum forces were significantly lower than those obtained during overground trials, even when the speeds of locomotion in the simulator were 66 % greater than those in 1 G. Cadence had no effect on any of the response variables. The maximum rate of force application (DFDT-Max) was similar for overground running and exercise in simulated 0G, provided that the "weightless subjects ran on a motorized treadmill. These findings have implications for the use of treadmill exercise as a countermeasure for hypokinetic osteoporosis. As the relationship between mechanical factors and osteogenesis becomes better understood, results from human experiments in 0G simulators will help to design in-flight exercise programs that are more closely targeted to generate appropriate mechanical stimuli.

Davis, B. L.↗