Genetically Fused Resilin-like Polypeptide–Coiled Coil Bundlemer Conjugates Exhibit Tunable Multistimuli-Responsiveness and Undergo Nanofibrillar Assembly
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Biochemistry & Molecular Biology↗
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Results for “genetics”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Inverse Design of Tetracene Polymorphs with Enhanced Singlet Fission Performance by Property-Based Genetic Algorithm Optimization
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
A Field Trial of TCE Phytoremediation by Genetically Modified Poplars Expressing Cytochrome P450 2E1
A controlled field study was performed to evaluate the effectiveness of transgenic poplars for phytoremediation. Three hydraulically contained test beds were planted with 12 transgenic poplars, 12 wild type (WT) poplars, or left unplanted, and dosed with equivalent concentrations of trichloroethylene (TCE). Removal of TCE was enhanced in the transgenic tree bed, but not to the extent of the enhanced removal observed in laboratory studies. Total chlorinated ethene removal was 87% in the CYP2E1 bed, 85% in the WT bed, and 34% in the unplanted bed in 2012. Evapotranspiration of TCE from transgenic leaves was reduced by 80% and diffusion of TCE from transgenic stems was reduced by 90% compared to WT. Cis-dichloroethene and vinyl chloride levels were reduced in the transgenic tree bed. Finally, chloride ion accumulated in the planted beds corresponding to the TCE loss, suggesting that contaminant dehalogenation was the primary loss fate.
59 BASIC BIOLOGICAL SCIENCES↗
Cluster-MLP: An Active Learning Genetic Algorithm Framework for Accelerated Discovery of Global Minimum Configurations of Pure and Alloyed Nanoclusters
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Modeling Metallic Halide Local Structures in Salt Melts Using a Genetic Algorithm
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Chemistry↗
Reduction of Exciton Diffusion Length with Genetically Tuned Non-Photochemical Quenching in Plant Thylakoid Membranes
Non-photochemical quenching (NPQ) protects plants from excess light by dissipating excitation energy as heat. Limited exciton migration is a key feature of NPQ, reducing the energy flux to reaction centers; however, quantitative evidence for this mechanism in native thylakoid membranes has been lacking. Here, we investigate the correlation between NPQ activity and exciton diffusion length (LD) using Nicotiana benthamiana mutants with distinct NPQ capacities. NPQ under both light- and dark-acclimated conditions was quantified for each genotype via fluorescence lifetime snapshots, and exciton mobility was probed using transient absorption spectroscopy with exciton-exciton annihilation analysis. By comparing the relationship between chlorophyll fluorescence lifetime and LD across mutants, we observed that NPQ activation quantitatively limits the spatial range of exciton migration, thereby reducing the access to reaction centers. Our findings provide direct experimental evidence that NPQ modulates the dynamics of energy transport, advancing our understanding of photoprotective regulation in photosynthetic systems.
Diffusion↗
Stimuli-Responsive Genetically Engineered Polymer Hydrogel Demonstrates Emergent Optical Responses
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Material Science↗
Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons
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Biochemistry & Molecular Biology↗
Characterization, Structure, and Inhibition of the Human Succinyl-CoA:glutarate-CoA Transferase, a Putative Genetic Modifier of Glutaric Aciduria Type 1
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Biochemistry & Molecular Biology↗
Pursuit of the Ultimate Regression Model for Samarium(III), Europium(III), and LiCl Using Laser-Induced Fluorescence, Design of Experiments, and a Genetic Algorithm for Feature Selection
Not Available
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose
Not Available
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Genetically engineered oleaginous yeast Lipomyces starkeyi for sesquiterpene a-zingiberene production
In this study we demonstrate that transgenic Lipomyces starkeyi strains expressing a ?-zingiberene synthase gene from Lemon basil or Hall’s panicgrass can produce up to 17 mg/l of ?-zingiberene in yeast extract peptone dextrose (YPD) medium containing 4% glucose. Following these findings, the transgenic strain was examined in 8% glucose media with C/N ratios of 20 or 80, and YPD. YPD medium resulted in 59.2 mg/l??-zingiberene accumulation. We further improved ?-zingiberene production by over-expression of selected genes from mevalonate pathway and achieved 145% improvement in ?-zingiberene synthesis. The growth medium was optimized for ?-zingiberene production, which resulted in 14.9% higher titer than YPD medium. The final transgenic strain produced 700 mg/l ?-zingiberene in fed-batch bioreactor culture. This study opens a new synthetic route to produce ?-zingiberene or other terpenoids in the oleaginous yeast L. starkeyi and establishes this yeast as a platform for jet fuel biosynthesis.
Dai, Ziyu↗
A Droplet Microfluidic Platform for Automating Genetic Engineering
We present a water-in-oil droplet microfluidic platform for transformation, culture and expression of recombinant proteins in multiple host organisms including bacteria, yeast and fungi. The platform consists of a hybrid digital microfluidic/channel-based droplet chip with integrated temperature control to allow complete automation and integration of plasmid addition, heat-shock transformation, addition of selection medium, culture, and protein expression. The microfluidic format permitted significant reduction in consumption (100-fold) of expensive reagents such as DNA and enzymes compared to the benchtop method. Furthermore, the chip contains a channel to continuously replenish oil to the culture chamber to provide a fresh supply of oxygen to the cells for long-term (~5 days) cell culture. The flow channel also replenished oil lost to evaporation and increased the number of droplets that could be processed and cultured. The platform was validated by transforming several plasmids into Escherichia coli including plasmids containing genes for fluorescent proteins GFP, BFP and RFP; plasmids with selectable markers for ampicillin or kanamycin resistance; and a Golden Gate DNA assembly reaction. We also demonstrate the applicability of this platform for transformation in widely used eukaryotic organisms such as Saccharomyces cerevisiae and Aspergillus Niger. Duration and temperatures of the microfluidic heat-shock procedures were optimized to yield transformation efficiencies comparable to those obtained by benchtop methods with a throughput up to 6 droplets/min. The platform we proposed offers potential for automation of molecular biology experiments significantly reducing cost, time and variability while improving throughput.
59 BASIC BIOLOGICAL SCIENCES↗
NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria
Not provided.
Biochemistry & Molecular Biology↗
High-CO 2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria
As researchers engineer cyanobacteria for biotechnological applications, we must consider potential environmental release of these organisms. Previous theoretical work has considered cyanobacterial containment through elimination of the CO 2 -concentrating mechanism (CCM) to impose a high-CO 2 requirement (HCR), which could be provided in the cultivation environment but not in the surroundings. In this work, we experimentally implemented an HCR containment mechanism in Synechococcus sp. strain PCC7002 (PCC7002) through deletion of carboxysome shell proteins and showed that this mechanism contained cyanobacteria in a 5% CO 2 environment. We considered escape through horizontal gene transfer (HGT) and reduced the risk of HGT escape by deleting competence genes. We showed that the HCR containment mechanism did not negatively impact the performance of a strain of PCC7002 engineered for L-lactate production. In conclusion, we showed through coculture experiments of HCR strains with ccm-containing strains that this HCR mechanism reduced the frequency of escape below the NIH recommended limit for recombinant organisms of one escape event in 10 8 CFU.
59 BASIC BIOLOGICAL SCIENCES↗
Genetic Selection for Small Molecule Production in Competitive Microfluidic Droplets
Biosensors can be used to screen or select for small molecule production in engineered microbes. However, mutations to the biosensor that interfere with accurate signal transduction are common, producing an excess of false positives. Strategies have been developed to avoid this limitation by physically separating the production pathway and biosensor, but these approaches have only been applied to screens, not selections. In this work, we have developed a novel biosensor-mediated selection strategy using competition between cocultured bacteria. When applied to the biosynthesis of cis,cis-muconate, we show that this strategy yields a selective advantage to producer strains that outweighs the costs of production. By encapsulating the competitive cocultures into microfluidic droplets, we successfully enriched the muconate-producing strains from a large population of control nonproducers. Finally, facile selections for small molecule production will increase testing throughput for engineered microbes and allow for the rapid optimization of novel metabolic pathways.
59 BASIC BIOLOGICAL SCIENCES↗