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At least 1,261 records · Page 70

Initial Biological Damage from Space Radiation: Implications for Development of Biological Countermeasures

Astronauts are exposed to high-energy nuclear particles originating from the galactic cosmic rays, high-energy protons trapped in the Earth's magnetic field or solar particle events, and secondary radiation produced by nuclear reactions. Important differences between conventional radiation including X-rays or gamma-rays, and high-energy nuclei occur at the level of initial damage to DNA and other potential biological target molecules, and to tissues. Such differences include a large fraction of the initial damage from high charge and energy (HZE) nuclear particles manifested as irreparable lesions including small- and large-scale DNA deletions. Also, low dose-rate exposures in space result in a heterogeneous population of damaged cells distinct from energetic photon irradiation of tissue. We present an overview of the initial biological damage and dose and dose-rate effects produced by ionizing radiation using track structure and nuclear reaction models. Implications of the differences in cellular and tissue damage between conventional radiation and space radiation for the development of biological countermeasures are discussed.

Cucinotta, Francis A.↗

Response Versus Scan-Angle Corrections for MODIS Reflective Solar Bands Using Deep Convective Clouds

The absolute radiometric calibration of the reflective solar bands (RSBs) of Aqua- and Terra-MODIS is performed using on-board calibrators. A solar diffuser (SD) panel along with a solar diffuser stability monitor (SDSM) system, which tracks the performance of the SD over time, provides the absolute reference for calibrating the MODIS sensors. MODIS also views the moon and deep space through its space view (SV) port for lunar-based calibration and computing the zero input radiance, respectively. The MODIS instrument views the Earth's surface through a two-sided scan mirror, whose reflectance is a function of angle of incidence (AOI) and is described by response versus scan-angle (RVS). The RVS for both MODIS instruments was characterized prior to launch. MODIS also views the SD and the moon at two different assigned RVS positions. There is sufficient evidence that the RVS is changing on orbit over time and as a function of wavelength. The SD and lunar observation scans can only track the RVS variation at two RVS positions. Consequently, the MODIS Characterization Support Team (MCST) developed enhanced approaches that supplement the onboard calibrator measurements with responses from pseudo-invariant desert sites. This approach has been implemented in Level 1B (L1B) Collection 6 (C6) for selected short-wavelength bands. This paper presents an alternative approach of characterizing the mirror RVS to derive the time-dependent RVS correction factors for MODIS RSBs using tropical deep convective cloud (DCC) targets. An initial assessment of the DCC response from Aqua-MODIS band 1 C6 data indicates evidence of RVS artifacts, which are not uniform across the scans and are more prevalent in the left side Earth-view scans.

Bhatt, Rajendra↗

Response Versus Scan-Angle Corrections for MODIS Reflective Solar Bands Using Deep Convective Clouds

The absolute radiometric calibration of the reflective solar bands (RSBs) of Aqua- and Terra-MODIS is performed using on-board calibrators. A solar diffuser (SD) panel along with a solar diffuser stability monitor (SDSM) system, which tracks the performance of the SD over time, provides the absolute reference for calibrating the MODIS sensors. MODIS also views the moon and deep space through its space view (SV) port for lunar-based calibration and computing the zero input radiance, respectively. The MODIS instrument views the Earths surface through a two-sided scan mirror, whose reflectance is a function of angle of incidence (AOI) and is described by response versus scan-angle (RVS). The RVS for both MODIS instruments was characterized prior to launch. MODIS also views the SD and the moon at two different assigned RVS positions. There is sufficient evidence that the RVS is changing on orbit over time and as a function of wavelength. The SD and lunar observation scans can only track the RVS variation at two RVS positions. Consequently, the MODIS Characterization Support Team (MCST) developed enhanced approaches that supplement the onboard calibrator measurements with responses from pseudo-invariant desert sites. This approach has been implemented in Level 1B (L1B) Collection 6 (C6) for selected short-wavelength bands. This paper presents an alternative approach of characterizing the mirror RVS to derive the time-dependent RVS correction factors for MODIS RSBs using tropical deep convective cloud (DCC) targets. An initial assessment of the DCC response from Aqua-MODIS band 1 C6 data indicates evidence of RVS artifacts, which are not uniform across the scans and are more prevalent in the left side Earth-view scans.

Bhatt, Rajendra↗

Precision X-band Doppler and ranging navigation for current and future Mars exploration missions

This paper describes a navigation error covariance analysis of two scenarios derived from the Mars Observer mission and the planned Mars Environmental SURvey (MESUR) Pathfinder mission, respectively. The analysis was performed to establish the potential navigational performance of the X-band tracking system in NASA's Deep Space Network, and to evaluate the sensitivity of the predicted performance to variations in the quantity of data acquired, the ground system error modeling assumptions, and data reduction schemes. The simulated data arcs used in the analysis are representative of the actual data arcs that will be used to predict the aim point for the Mars orbit insertion maneuver, in the case of Mars Observer, and the interplanetary trajectory aim point corresponding to the target Mars landing site in the case of MESUR Pathfinder. The results indicate that with a suitable sequential data reduction scheme and accurate calibrations of instrumentation, transmission media, and platform model parameters, navigation accuracies of 5 to 15 km (1 sigma) can be achieved, equivalent to 15 to 40 nrad in geocentric angle uncertainty.

Estefan, Jeffrey A.↗

NISAR L-SAR Digital Electronics Subsystem - A Multichannel Distributed Processing System with Synchronous Timing Control for Digital Beam Forming and Multiple Echo Tracking

The NASA-ISRO Synthetic Aperture Radar (NISAR) L-band SAR instrument employs multiple digital channels to optimize resolution while keeping a large swath on a single pass. High-speed digitization with fine synchronization and digital beam forming are necessary in order to facilitate this new technique called SweepSAR. An architecture employing multiple FPGA based digital signal processors has been conceived to facilitate digital calibration on an individual channel basis as well as digital signal processing to optimize the receive signal. On-board processing and data compression has been implemented to reduce the volume of data in order to satisfy the operational requirements of near global coverage for the desired science targets. A novel command and timing architecture was developed to manage this complex system while providing detailed control of individual channel receive window timing required for digital beam forming. The NISAR L-band Digital Electronics Subsystem is the combination of the hardware, firmware and software components architected and implemented to operate this radar and return the desired quantity and quality of data for the science community.

Chuang, Chung-Lun↗

Process Anomaly Detection for Sparsely Labeled Events in Nuclear Power Plants

An essential aspect of online monitoring, subtle anomaly detection increases the detection lead time for equipment failure and enables a nuclear power plant (NPP) to mitigate unexpected partial or full outages, resulting in significant cost saving to the plant. Once an anomaly is detected by plant staff, its cause and severity are investigated. Because the vast majority of anomalies require some level of investigation, including some that require time-consuming examination, before they are passed over to the engineering organization for further analysis, plants are often equipped with tools to assist the staff in performing anomaly detection. Those tools operate as a black box and are often based on statistical methods that establish sensor correlations using preconfigured mathematical models and flag correlation deviations as anomalies. Due to the number of anomalies detected at a given NPP on a daily basis, a significant number of flagged anomalies usually await examination for days or weeks. A primary cause of this backlog is that the methods used by the tools generate many false positives. Though this is usually attributed to oversensitive model settings due to very narrow normal operation bands, it can also be associated with the model development being inadequate for the process being monitored, or with missing model inputs that could have explained misclassified positives. The performance of anomaly detection tools impacts their plant acceptance and utilization, especially when the effort to address false positives generated by the tool depletes the value or cost saved by using that tool. Thus, means to advance anomaly detection performance have been investigated by the Department of Energy’s Light Water Reactor Sustainability program. Previous and ongoing efforts have targeted unsupervised machine-learning (ML) methods, which do not require the labeling of any data fed into the ML model. By contrast, in supervised anomaly detection methods, every data point is labeled as either a normal or abnormal process condition, and the model is trained to replicate the classification process. Supervised methods usually outperform unsupervised methods, due to the added value in differentiating normal from anomalous states of the monitored process. An NPP’s corrective action program requires it to track and document, via a dedicated report, the resolution of any issues that occur within the plant. Once created, each report is reviewed by a plant screening committee, and several classifications and decisions are made. Recently, a collaborating NPP developed an artificial intelligence and ML-based classifier to categorize a condition report (CR) into classes that can serve to label the data as normal or anomalous. Applying CRs as labels represents a semi-supervised use case. Semi-supervised ML assumes that labels exist for some data points (i.e., labeled anomalies, in this case) but not for the rest. In this effort, semi-supervised ML methods were used to fuse data from CRs with anomaly detection methods in order to test the hypothesis that partially labeled anomalies would improve the accuracy of the anomaly detection methods. Specifically, two methods were used. The first is the deep Semi-supervised Anomaly Detection (deep SAD) method, which can handle labels ranging from fully unsupervised to fully supervised cases. The second is a newly designed ML method developed specifically for this effort and referred to as the high-order feature (HOF)-based method. To evaluate these two methods in controlled environments, synthetic data generators were developed and used. The first datasets used a spring-mass-damper (SMD) system simulator commonly found in mechanical engineering references. This was used to create two use cases: a one- and a three-mass system. Anomalies were introduced by changing the spring and damper coefficients while the system was actuated by random forces. The second datasets used the commercial Dymola-Modelica software to build a simplified nuclear reactor model. Anomalies were added in the form of corrupted sensor readings and/or control commands. The deep SAD method was tested using the SMD system, while the HOF method was tested using both datasets. Application of the deep SAD semi-supervised ML method demonstrated that labels can generate increased confidence in detecting true anomalies. This helped increase the number of true positives and decrease the number of false negatives—something that would aid in addressing the backlog of possible anomalies. Application of the HOF method demonstrated that labels can aid in down selecting from a candidate set of features to a more optimal subset in order to better differentiate between normal and anomalous conditions.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Towards Energy Scale Calibration and Drift Correction of TES Detectors for Athena X-IFU

The Athena X-Ray Integral Field Unit (X-IFU) comprises a 2376-pixel array of transition edge sensors (TES) read out with time-division multiplexing (TDM). X-IFU will provide spatially resolved, high-resolution spectroscopy (2.5 eV full-width-half-maximum up to 7 keV) over the energy range 0.2 to 12 keV, with an absolute energy scale accuracy of 0.4 eV. The energy scale function maps the optimally filtered pulse height, in arbitrary engineering units, to real calibrated energy. Uncertainties in the calibration can result from imperfect fitting of the energy scale between the known calibration points. Furthermore, temporal changes in the TES operating environment, such as heat-sink temperature, magnetic field and bias voltage, can cause significant variations in the detector gain function over time. If not properly corrected, this can result in degradation of the energy resolution, and systematic errors in the absolute energy scale. The non-linear nature of TES detectors, coupled with the possibility of multiple simultaneously occurring sources of drift, can make effective corrections over the full bandpass of the instrument extremely challenging. Athena X-IFU will employ an on-board calibration source that provides known reference x-ray lines. This provides real-time monitoring of the gain stability of the detector system and information that can be used to correct for gain drifts. For X-IFU the baseline approach is to measure a series of calibration curves under different environmental conditions, which bound the expected drifts the instrument is predicted to see over the course of the mission. Using the information from the in-flight calibration source, these energy scale functions can be interpolated to generate a new corrected energy scale as a function of time. In this paper we discuss progress towards demonstrating that the X-IFU energy scale requirements can be met. We present measurements on ~ 200 pixels in a prototype X-IFU array read out with 8-column x 32-row TDM. We use a rotating target source containing 12 fluorescent targets to generate x-ray lines covering the energy range 4 keV (Sc-Kα) to 12 keV (Br-Kα). We present measurements of the non-linear energy scale function and show how variations in heat-sink temperature, TES bias voltage and magnetic field affect the shape of TES energy scale differently and introduce different residual gain errors over the bandpass. We explore different drift correction algorithms that use either a single or multiple referential lines to track and correct the gain from these various sources of drift. In addition to the pulse-height, the DC ‘baseline’ level of the TES can contain information about its bias conditions. Thus, we test a multi-parameter gain correction algorithm that attempts to incorporate both the pulse height and the additional baseline information into the algorithm.

Stephen J Smith↗

A Real-Time Data Dashboard for Monitoring Travel Behavior

When running a smartphone app-based travel behavior study, it is important to keep participants engaged in contributing data. Long study durations and/or large samples make it difficult to engage participants in reporting their travel behavior. We developed a dashboard web application called 'emdash' to facilitate and ease the data collection maintenance task. It allows deployers to view trip trajectories, track which participants are uploading data, and generate report ready plots. Emdash can also be useful for deployers to identify ways to best encourage a particular transportation mode shift and to provide feedback and reporting to participants. Using the dashboard, a single admin was able to support a classic travel study with 80 participants. The dashboard is currently deployed in a two-year electric bike (e-bike) pilot study called CanBikeCO, conducted in six locations across Colorado, USA. CanBikeCO targets carbon emissions reduction and equity improvement by evaluating changes to participants' travel behavior when they are offered free e-bikes. Each CanBikeCO location has access to their own version of emdash to help them monitor and support participants. After testing and gathering feedback, we made improvements to the dashboard's usability and scalability, and proposed a method to make detecting data issues easier. Improvements include configurable options and limiting the volume of data sent to the dashboard as study size and duration increase.

ADVANCED PROPULSION SYSTEMS,MATHEMATICS AND COMPUT↗

SILVER: Surface Imaging for Lunar Volatiles, Resources, and Exploration

The Surface Imaging for Lunar Volatiles, Exploration, and Resources (SILVER) instrument is a proposed imaging investigation for the 2008 Lunar Reconnaissance Orbiter (LRO) mission. SILVER and its experienced Measurement Team will prepare for and support future lunar human exploration activities, especially landing site identification and certification on the basis of potential resources. SILVER combines a high-resolution pushbroom visible imaging channel (SILVER-HR) and a wide-field-of-view (45 deg) framing imaging channel (SILVER-WF). SILVER-HR will obtain a single-detector 6 km imaging swath of 12,228 pixels at 0.5 m/pixel to image greater than 100 sq km target areas from 50 km altitude, imaging greater than 15% the lunar surface during a 1 year nominal mission. SILVER-HR has excellent stray-light rejection and its imaging detector has selectable time delay integration (TDI) with up to 128 stages for extreme low-light sensitivity, permitting direct imaging of permanently shadowed polar regions in scattered sunlight or earthshine. SILVER-WF will obtain geodetic framing images in a 2048 x 2048 format at 20m/pixel, with 60% along-track overlap stereo for imaging context and for derivation of a global digital elevation model of meter-scale lunar topography.

Pappalardo, R. T.↗

Exploring Benchmarks for Self-Driving Labs using Color Matching

Self Driving Labs (SDLs) that combine automation of experimental procedures with autonomous decision making are gaining popularity as a means of increasing the throughput of scientific workflows. The task of identifying quantities of supplied colored pigments that match a target color, the color matching problem, provides a simple and flexible SDL test case, as it requires experiment proposal, sample creation, and sample analysis, three common components in autonomous discovery applications. We present a robotic solution to the color matching problem that allows for fully autonomous execution of a color matching protocol. Our solution leverages the WEI science factory platform to enable portability across different robotic hardware, the use of alternative optimization methods for continuous refinement, and automated publication of results for experiment tracking and post-hoc analysis.

Artificial intelligence↗

The effect of transient needle lift on the internal flow and near-nozzle spray characteristics for modern GDI systems investigated by high-speed X-ray imaging

The development of the injector nozzle is a dynamic area in regard of several technical aspects. At first, the internal flow influences the near-field spray characteristics via various phenomena such as cavitation and turbulence. However, these phenomena are not fully understood due to their extremely fast, complex and multiscale nature. Furthermore, it governs the spray targeting inside the combustion chamber. High-speed X-ray imaging of GDI injector nozzles is performed in this study. The experimental results presented are related to the internal flow and primary breakup of discharged liquid jets. The injectors used are equipped with nozzles made of aluminum which have been specially developed for these investigations to enhance optical accessibility. The visualization of the needle motion, in-nozzle flow and the primary breakup region provides several exciting observations. First, the needle lift tracking exhibits short overshooting right before the steady-state of the injection phase. Additionally, this event leads to a short-term, however, significant change in the associated performance of the breakup. This phenomenon is found to be a consequence of the transient behavior of the in-nozzle flow. It is shown that under some circumstances hydraulic flip may occur during this overshooting period. The primary jet breakup region is visualized and evaluated by means of image processing. Thus, the transient behavior of liquid jet expansion is quantified in the vicinity of the nozzle. It is observed that the liquid jet direction deviates from the hole axis already at the nozzle outlet, which is caused by internal flow characteristics.

42 ENGINEERING↗

Assessment of RFID Read Accuracy for ISS Water Kit

The Space Life Sciences Directorate/Medical Informatics and Health Care Systems Branch (SD4) is assessing the benefits Radio Frequency Identification (RFID) technology for tracking items flown onboard the International Space Station (ISS). As an initial study, the Avionic Systems Division Electromagnetic Systems Branch (EV4) is collaborating with SD4 to affix RFID tags to a water kit supplied by SD4 and studying the read success rate of the tagged items. The tagged water kit inside a Cargo Transfer Bag (CTB) was inventoried using three different RFID technologies, including the Johnson Space Center Building 14 Wireless Habitat Test Bed RFID portal, an RFID hand-held reader being targeted for use on board the ISS, and an RFID enclosure designed and prototyped by EV4.

Chu, Andrew↗

Comparing Pre-Launch Assumptions to In-Flight Navigation Performance of OSIRIS-REx

The Sample Return Capsule (SRC) onboard the NASA Origins, Spectral Inter-pretation, Resource Identification, and Security–Regolith Explorer (OSIRIS-REx)spacecraft is currently carrying samples of the B-type asteroid Bennu for safe re-turn to Earth at the Utah Test and Training Range on September 24, 2023. These samples were collected during the Touch And Go (TAG) sampling event on October 20, 2020, when the spacecraft contacted the surface for a few seconds at a location less than 1 meter from the target. The unprecedented navigation performance achieved during that event was the culmination of experience gained during two years of cruise and two years of increasingly challenging operation sat Bennu. As we had hoped, the proximity navigation performance at Bennu exceeded pre-launch analysis. This paper will compare the navigation performance through the proximity operation phases to our pre-launch analysis and will quantify how refinements of the small force models governing the spacecraft motion near Bennu considerably improved the down-track state predictions leading up to the successful TAG event. It was evident to the team and to expert peer reviewers during the design phase that exquisite model fidelity and aggressive operational concepts, which challenged and advanced the state of the art for deep space proximity operations, would be required to meet the mission’s objectives. This paper summarizes the superlative achievements of the team in rising to and overcoming these challenges.

Peter G Antresian↗

Application of X-Ray Pulsar Navigation: A Characterization of the Earth Orbit Trade Space

The potential for pulsars as a navigation source has been studied since their discovery in 1967. X-ray pulsar navigation (XNAV) is a celestial navigation system that uses the consistent timing nature of x-ray photons from milli-second pulsars (MSP) to perform space navigation. By comparing the detected arrival of x-ray photons to a reference database of expected pulsar lightcurve timing models, one can infer a range and range rate measurement based on light time delay. Much of the challenge of XNAV comes from the faint signal, availability, and distant nature of pulsars. This is a study of potential pulsar XNAV measurements to measure extended Kalman filter (EKF) tracking performance with a wide trade space of bounded Earth orbits, using a simulation of existing x-ray detector space hardware. An example of an x-ray detector for XNAV is the NASA Station Explorer for X-ray Timing and Navigation (SEXTANT) mission, a technology demonstration of XNAV set to perform on the International Space Station (ISS) in late 2016early 2017. XNAV hardware implementation is driven by trajectory and environmental influences which add noise to the x-ray pulse signal. In a closed Earth orbit, the radiation environment can exponentially increase the signal noise from x-ray pulsar sources, decreasing the quality and frequency of measurements. The SEXTANT mission in particular improves on the signal to noise ratio by focusing an array of 56 x-ray silicon drift detectors at one pulsar target at a time. This reduces timing glitches and other timing noise contributions from ambient x-ray sources to within a 100 nanosecond resolution. This study also considers the SEXTANT scheduling challenges inherent in a single target observation. Finally, as the navigation sources are now relatively inertial targets, XNAV measurements are also subject to periods of occultation from various celestial bodies. This study focuses on the characterization of these drivers in closed Earth orbits and is not a tuning analysis of the EKF. The study shows that the closed Earth orbit for XNAV performance is reliant on the orbit semi-major axis and eccentricity as well as orbit inclination. These parameters are the primary drivers of pulsar measurement availability and significantly influence the natural spacecraft orbit dynamics. Sensitivity to initial orbit determination error growth due to the scarcity of XNAV measurements within an orbital period require appropriate timing of initial XNAV measurements. The orbit angles of argument of perigee and right ascension of the ascending node, alongside the other orbit parameters, complete the initial cadence of XNAV measurements. The performance of initial XNAV measurements then propagates throughout the experimental period. The study provides a basis to missions who wish to consider XNAV as a potential navigation source in a closed Earth orbit design. It provides a family of orbit trajectories as well as other modeling considerations needed to effectively evaluate if XNAV is an effective navigation source for a potential mission. As an EKF is sensitive to a linearized estimated state, this study has a direct benefit of providing effective XNAV measurements to maintain spacecraft tracking, independent of other navigation sources. In the particular use case of the SEXTANT mission, it also provides a novel scheduling algorithm which addresses the need to prioritize and manage pulsar observations for effective navigation.

PULSAR↗

Application of X-Ray Pulsar Navigation: A Characterization of the Earth Orbit Trade Space

The potential for pulsars as a navigation source has been studied since their discovery in 1967. X-ray pulsar navigation (XNAV) is a celestial navigation system that uses the consistent timing nature of x-ray photons from millisecond pulsars (MSP) to perform space navigation. By comparing the detected arrival of x-ray photons to a reference database of expected pulsar light-curve timing models, one can infer a range and range rate measurement based on light time delay. Much of the challenge of XNAV comes from the faint signal, availability, and distant nature of pulsars. This is a study of potential pulsar XNAV measurements to measure extended Kalman filter (EKF) tracking performance with a wide trade space of bounded Earth orbits, using a simulation of existing x-ray detector space hardware. An example of an x-ray detector for XNAV is the NASA Station Explorer for X-ray Timing and Navigation (SEXTANT) mission, a technology demonstration of XNAV set to perform on the International Space Station (ISS) in late 2016early 2017. XNAV hardware implementation is driven by trajectory and environmental influences which add noise to the x-ray pulse signal. In a closed Earth orbit, the radiation environment can exponentially increase the signal noise from x-ray pulsar sources, decreasing the quality and frequency of measurements. The SEXTANT mission in particular improves on the signal to noise ratio by focusing an array of 56 x-ray silicon drift detectors at one pulsar target at a time. This reduces timing glitches and other timing noise contributions from ambient x-ray sources to within a 100 nanosecond resolution. This study also considers the SEXTANT scheduling challenges inherent in a single target observation. Finally, as the navigation sources are now relatively inertial targets, XNAV measurements are also subject to periods of occultation from various celestial bodies. This study focuses on the characterization of these drivers in closed Earth orbits and is not a tuning analysis of the EKF. The study shows that the closed Earth orbit for XNAV performance is reliant on the orbit semi-major axis and eccentricity as well as orbit inclination. These parameters are the primary drivers of pulsar measurement availability and significantly influence the natural spacecraft orbit dynamics. Sensitivity to initial orbit determination error growth due to the scarcity of XNAV measurements within an orbital period require appropriate timing of initial XNAV measurements. The orbit angles of argument of perigee and right ascension of the ascending node, alongside the other orbit parameters, complete the initial cadence of XNAV measurements. The performance of initial XNAV measurements then propagates throughout the experimental period. The study provides a basis to missions who wish to consider XNAV as a potential navigation source in a closed Earth orbit design. It provides a family of orbit trajectories as well as other modeling considerations needed to effectively evaluate if XNAV is an effective navigation source for a potential mission. As an EKF is sensitive to a linearized estimated state, this study has a direct benefit of providing effective XNAV measurements to maintain spacecraft tracking, independent of other navigation sources. In the particular use case of the SEXTANT mission, it also provides a novel scheduling algorithm which addresses the need to prioritize and manage pulsar observations for effective navigation.

x-ray↗

Photoelectron–residual-ion interaction in angle-resolved streaked shake-up ionization of helium

Streaked photoelectron emission spectra access the correlated dynamics of photoelectrons and residual target electrons with attosecond temporal resolution. Here, we calculated ab initio single-ionization spectra for photoemission from helium atoms by colinearly polarized ultrashort XUV and assisting few-femtosecond IR pulses. Distinguishing direct and shake-up ionization resulting in ground-state and excited (𝑛 = 2, 3) He + residual ions, respectively, we examined the effects of the correlated photoemission dynamics on the photoelectron phase accumulation as a function of the observable photoelectron detection direction and kinetic energy and XUV–IR pulse delay. We tracked the dynamical evolution of the residual ion in relative streaked photo- emission delays and found dominant contributions for shake-up emission from the residual-ion–photoelectron interaction. These are in very good and fair agreement, respectively, for 𝑛 = 2 and 𝑛 = 3 shake-up photoemission along the pulse-polarization directions, with previous experimental and theoretical investigations [Ossiander et al ., Nat. Phys. 13, 280 (2017)] and reveal a strong photoemission-direction dependence for shake-up ionization due to the coupling between the photoelectron and evolving residual-ion charge distribution in the IR-laser field.

Stark effect↗

Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR

CRISPR/Cas9 based genome editing has advanced our understanding of a myriad of important biological phenomena. Important challenges to multiplex genome editing in maize include assembly of large complex DNA constructs, few genotypes with efficient transformation systems, and costly/labor-intensive genotyping methods. Here we present an approach for multiplex CRISPR/Cas9 genome editing system that delivers a single compact DNA construct via biolistics to Type I embryogenic calli, followed by a novel efficient genotyping assay to identify desirable editing outcomes. We first demonstrate the creation of heritable mutations at multiple target sites within the same gene. Next, we successfully created individual and stacked mutations for multiple members of a gene family. Genome sequencing found off-target mutations are rare. Multiplex genome editing was achieved for both the highly transformable inbred line H99 and Illinois Low Protein1 (ILP1), a genotype where transformation has not previously been reported. In addition to screening transformation events for deletion alleles by PCR, we also designed PCR assays that selectively amplify deletion or insertion of a single nucleotide, the most common outcome from DNA repair of CRISPR/Cas9 breaks by non-homologous end-joining. The Indel-Selective PCR (IS-PCR) method enabled rapid tracking of multiple edited alleles in progeny populations. The ‘end to end’ pipeline presented here for multiplexed CRISPR/Cas9 mutagenesis can be applied to accelerate maize functional genomics in a broader diversity of genetic backgrounds.

59 BASIC BIOLOGICAL SCIENCES↗

Data for "Efficient Mutagenesis and Genotyping of Maize Inbreds Using Biolistics, Multiplex CRISPR/Cas9 Editing, and Indel-Selective PCR"

CRISPR/Cas9 based genome editing has advanced our understanding of a myriad of important biological phenomena. Important challenges to multiplex genome editing in maize include assembly of large complex DNA constructs, few genotypes with efficient transformation systems, and costly/labor-intensive genotyping methods. Here we present an approach for multiplex CRISPR/Cas9 genome editing system that delivers a single compact DNA construct via biolistics to Type I embryogenic calli, followed by a novel efficient genotyping assay to identify desirable editing outcomes. We first demonstrate the creation of heritable mutations at multiple target sites within the same gene. Next, we successfully created individual and stacked mutations for multiple members of a gene family. Genome sequencing found off-target mutations are rare. Multiplex genome editing was achieved for both the highly transformable inbred line H99 and Illinois Low Protein1 (ILP1), a genotype where transformation has not previously been reported. In addition to screening transformation events for deletion alleles by PCR, we also designed PCR assays that selectively amplify deletion or insertion of a single nucleotide, the most common outcome from DNA repair of CRISPR/Cas9 breaks by non-homologous end-joining. The Indel-Selective PCR (IS-PCR) method enabled rapid tracking of multiple edited alleles in progeny populations. The ‘end to end’ pipeline presented here for multiplexed CRISPR/Cas9 mutagenesis can be applied to accelerate maize functional genomics in a broader diversity of genetic backgrounds.

gene editing↗