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NASA NTRS2017

Future Food Production System Development Pulling From Space Biology Crop Growth Testing in Veggie

Preliminary crop testing using Veggie indicates the environmental conditions provided by the ISS are generally suitable for food crop production. When plant samples were returned to Earth for analysis, their levels of nutrients were comparable to Earth-grown ground controls. Veggie-grown produce food safety microbiology analysis indicated that space-grown crops are safe to consume. Produce sanitizing wipes were used on-orbit to further reduce risk of foodborne illness. Validation growth tests indicated abiotic challenges of insufficient or excess fluid delivery, potentially reduced air flow leading to excess water, elevated CO2 leading to physiological responses, and microorganisms that became opportunistic pathogens. As NASA works to develop future space food production, several areas of research to define these systems pull from the Veggie technology validation tests. Research into effective, reusable water delivery and water recovery methods for future food production systems arises from abiotic challenges observed. Additionally, impacts of elevated CO2 and refinement of fertilizer and light recipes for crops needs to be assessed. Biotic pulls include methods or technologies to effectively sanitize produce with few consumables and low inputs; work to understand the phytomicrobiome and potentially use it to protect crops or enhance growth; selection of crops with high harvest index and desirable flavors for supplemental nutrition; crops that provide psychosocial benefits, and custom space crop development. Planning for future food production in a deep space gateway or a deep space transit vehicle requires methods of handling and storing seeds, and ensuring space seeds are free of contaminants and long-lived. Space food production systems may require mechanization and autonomous operation, with preliminary testing initiated to identify operations and capabilities that are candidates for automation. Food production design is also pulling from Veggie logistics lessons, as we learn about growing at different scales and move toward developing systems that require less launch mass. Veggie will be used as a test bed for novel food production technologies. Veggie is a relatively simple precursor food production system but the knowledge gained from space biology validation tests in Veggie will have far reaching repercussions on future exploration food production. This work is supported by NASA.

Plants↗

NASA NTRS2018

Microbial Monitoring and the Risk of Infectious Disease Aboard the International Space Station

The International Space Station (ISS) is a semi-closed habitat in low Earth orbit with environmental conditions provided by an advanced life support system that controls temperature and recycles air and most of the potable water. The crew's activities, such as eating, sleeping, hygiene, and laboratory research, are performed in relatively close proximity. Research in the laboratory includes a myriad of experiments, including those with rodents, plants, and pathogenic microorganisms. Despite these conditions, in-flight monitoring of ISS indicates that the microbial diversity is similar to homes on earth. Accordingly, the crew is generally very healthy, however infectious disease does occur and potential routes of infection by obligate and opportunistic pathogens cannot be completely prevented. Determining the extent of this risk is further complicated, as microorganisms can alter their characteristics in response to spaceflight culture, as exemplified by the increase in virulence of the enteric pathogen Salmonella enterica Typhimurium during spaceflight compared to otherwise identical cultures grown on Earth. Taken together, these factors suggest a need for continued microbiological monitoring and research to understand and mitigate the risk of infectious disease during long duration missions.

Ott, C. Mark↗

NASA NTRS2018

Gene Expression of Pathogens in Simulated Microgravity

Extended exposure to radiation and microgravity in space has been linked to astronauts developing chronic diseases upon returning to Earth. The Gram-negative pathogen Serratia marcescens has been shown to potentially cause significant infections in humans and in insect models on Earth. Our recent findings also showed that S. marcescens shows an increase in virulence after a short period of growth in the spaceflight environment, which raises initiatives to find the correlation between space environment and the increased virulence. Because we know that the health of astronauts is immunocompromised in space, it is possible that the combination of increased bacterial virulence and the weakened immune system will cause astronauts to be more susceptible to chronic diseases in extended spaceflight. With 75% of human disease genes being conserved in the fruit fly Drosophila melanogaster, these insects act as an ideal model organism to study the human immune system. The high accessibility, low cost, high rate of reproductivity, and short lifespans of D. melanogaster facilitate efficient, high-quality research that seeks to understand altered virulence of this opportunistic pathogen. In this ground-based study, we will use a rotating wall vessel apparatus to simulate microgravity and determine how pathogenicity changes by evaluating differences in gene expression for S. marcescens between bacteria grown in simulated microgravity conditions and controls. We will compare the results of our findings to gene expression patterns in actual spaceflight samples of S. marcescens grown on the ISS (International Space Station) during a recent validation mission, to see if there are common mechanisms across our simulated microgravity and actual spaceflight microgravity samples that both show increased virulence in the fruit fly. With extended space travel in the foreseeable future, understanding how human physiology will be affected by these different factors will help mitigate risks and deaths.

spaceflight analogue↗

NASA NTRS2014

Exploration of Planetary Skylights and Tunnels

While planetary pits and caves have been fiction for a century, they have been seen from orbit only in the last few years. These discoveries exceed the fantasies in diversity, scale, and abundance. For pits and caves, this is the age of discovery, ranging from a few pits on the Moon and Mars in 2009 to hundreds within the time of this research, with many more to come. Pits with subsurface voids have been confirmed on the Moon and Mars and indicated on Venus, Phobos, Eros, Gaspra, Ida, Enceladus, and Europa. Compelling next steps are surface and subsurface exploration.Pits and caves are opportunistic study targets for unique origins, geology, and climate that will broadly impact planetary science. Holes on Mars are of particular interest because their interior caves are relatively protected from the harsh surface, making them good candidates to contain Martian life. Pits are prime targets for possible future spacecraft, robots, and even human interplanetary explorers. Caves and caverns could be ready-_made shelters for future Moon and Mars explorers and colonists. Discoveries to date look down from on high with satellites but cannot reveal the wonders of caves. They cannot enter, touch, or view pits up close. Genuine exploration is only achievable through surface missions. Robotic missions can assess suitability for safe entry and habitation, plus inform techniques for developing subsurface infrastructure.Missions into planetary voids redefine the future of exploration, science, and habitation beyond Earth. We can reach this future only by targeting specific technological advancement now. Prior missions and current roadmap priorities target regions of benign terrain. While in-cave concepts have been postulated, the critical technologies have not been identified and demonstrated.While robotic exploration of skylights and caves can seek out life, investigate geology and origins, and open the subsurface of other worlds to humankind, it is a daunting venture. Planetary voids present perilous terrain requiring innovative technologies for access, exploration, and modeling. These same technologies are broadly applicable to explorations of rough and/or subsurface planetary environments, including caves, craters, cliffs, and rock fields. This research speculates on the possibilities and means of such exploration with fundamental contributions to exploring, modeling, and visualizing this new class of large-scale, highly three-dimensional concave planetary features.

Whittaker, Red↗

NASA NTRS2019

Application of Machine Learning Techniques to Delay Tolerant Network Routing

This dissertation discusses several machine learning techniques to improve routing in delay tolerant networks (DTNs). These are networks in which there may be long one-way trip times, asymmetric links, high error rates, and deterministic as well as non-deterministic loss of contact between network nodes, such as interplanetary satellite networks, mobile ad hoc networks and wireless sensor networks. This work uses historical network statistics to train a multi-label classifier to predict reliable paths through the network. In addition, a clustering technique is used to predict future mobile node locations. Both of these techniques are used to reduce the consumption of resources such as network bandwidth, memory and data storage that is required by replication routing methods often used in opportunistic DTN environments. Thesis contributions include: an emulation tool chain developed to create a DTN test bed for machine learning, the network and software architecture for a machine learning based routing method, the development and implementation of classification and clustering techniques and performance evaluation in terms of machine learning and routing metrics.

Dudukovich, Rachel M.↗

NASA NTRS2019

Investigation of Biofilm Formation and Control for Spacecraft-An Early Literature Review

Bacterial biofilms are an important and often problematic aspect of life on earth and inspace. Biofilms of opportunistic pathogenic bacteria can lead to severe and costlycontamination problems that directly affect human health and long-term mission planning.Microbial contamination on board the International Space Station (ISS) continues to posemission risks, both to crew health and hardware reliability. In order to optimize the design offuture space exploration vehicles, a thorough understanding of biofilm formation and controltechnologies is needed to control the habitat's microbial environment. This paper provides aliterature review on microbial behavior, biofilm formation in spacecraft or simulatedspacecraft environments, and the state of the art of biofilm prevention mechanisms

Diaz, Angie M.↗

NASA NTRS2019

Planetary Science with Small Satellites: Opportunities and Challenges

Small satellites aren't anything particularly new. Earth orbiting small satellites go back 30 years or more. What is new is the proliferation and access to small satellite technologies and flight opportunities. This has been in large part due to the advent of the "cubesat" model, initially a means to develop student's engineering skills, but has since evolved into an industry and accepted method within government space agencies. Until very recently these smallsats were limited to Earth orbiting missions, but with the successful flight of the MarCO spacecraft and the upcoming launch of EM-1 cubesats, the Moon, Mars and beyond are now within reach. While all this is good news, we still have a ways to go before smallsats become true planetary science tools. One could argue that Deep Space 2 was the first planetary smallsat, launched in 1999 and having a mass of 2.3 kg (each probe) it hoped to demonstrate that "real" science could be done with a small (and less expensive) package. The DS2 failure shelved the idea of smallsats (even chilling some to "Class D" planetary missions in general) for nearly two decades. NASA has slowly come back around to smallsats for planetary missions, going so far as to support a range of mission studies (the Planetary Science Deep Space SmallSat Studies, or PSDS3, Program) and the creation of a new Program (SIMPLEx) to developed such missions for opportunistic flights. The MarCO success was hugely important in maintaining (and building) this forward momentum. However, we still have yet to demonstrate "real" science from a planetary smallsat and there are some fundamental disconnects between expectation and reality. This talk will discuss some of the opportunities and challenges that reside with planetary smallsats, focusing on two examples: LunaH-Map (the first SIMPLEx cubesat) and Aeolus (a Mars PSDS3 smallsat concept).

Colaprete, Anthony↗

NASA NTRS2019

Effects of Low-Shear Simulated Microgravity on Soil Bacterium Pantoea Agglomerans

A major stressor in the space environment is microgravity. Microgravity has profound effects on biological processes that are vital to normal functioning. This is most prevalently seen in microorganisms, which have altered growth rates and increased antibiotic susceptibility in microgravity. This is a concern for both astronauts and plants onboard spacecraft. Pantoea agglomerans is a soil bacterium that has been shown to be a plant growth promoter, plant pathogen, and an opportunistic pathogen to immunocompromised patients. Using the ground based microgravity analog, the Rotary Cell Culture System along with the High Aspect-Ratio Vessel, we analyzed the growth and the antibiotic susceptibility of Pantoea agglomerans grown in simulated microgravity. In certain parameters, we discovered an increased growth rate and no change in the antibiotic susceptibility. We found that there were differences in results when certain aspects of the protocol were altered. Further work will need to be conducted to get a better understanding of the changes in the microorganisms exposed to microgravity.

Microbial growth↗

NASA NTRS2017

Microbial Characteristics of ISS Environmental Surfaces

The microbiome of environmental surfaces from the International Space Station were characterized in order to examine the relationship to crew and hardware maintenance. The Microbial Observatory (ISS-MO) experiment generated a microbial census of ISS environments using advanced molecular microbial community analyses along with traditional culture-based methods. Since the “omics” methodologies generated an extensive microbial census, significant insights into spaceflight-induced changes in the populations of beneficial and/or potentially harmful microbes were gained. Surface samples were collected from several ISS surface locations from three flight opportunities, and were returned to Earth via the Soyuz TMA-14M or the Space X Dragon capsule. In addition to cultivation methods, viable microbial burden, iTag-based sequencing, and metagenome analyses were carried out. The cultivable microbial bioburden differed by location and sampling event. Exploring the ISS environmental microbiome revealed presence of opportunistic pathogens and antibiotic resistant microbes. Genes involved in ATP binding cassette transporters, two component systems, and beta-lactam resistance were among a diverse set of metabolic and genetic information processing pathways. Whole genome sequencing (WGS) of 50 ISS strains exhibiting resistance to various antibiotics was carried out. The antibiotic resistant genes deduced from the WGS were compared with the resistomes generated directly from the gene pool of the environmental samples. Two unique Aspergillus fumigatus strains isolated from the ISS were characterized and compared to the experimentally established clinical isolates Af293 and CEA10. A virulence assessment in a neutrophil-deficient larval zebrafish model of invasive aspergillosis indicated that both ISSFT-021 and IF1SW-F4 were significantly more lethal compared to Af293 and CEA10. The findings from this Environmental “Omics” project should be exploited to enhance human health and well-being of a closed system. In other words, the ISS-MO research aims to "translate" findings in fundamental research into medical practice (pathogen detection) and meaningful health outcomes (countermeasure development).

Perry, Jay↗

NASA NTRS2018

Mars Planetary Network for Human Exploration Era – Potential Challenges and Solutions

During 2016-2017, a study was conducted under the sponsorship of the NASA’s Space Communications and Navigation (SCaN) Program to investigate the deep space communications capacity taking into account the needs of all the present and envisioned future missions toward 2030s. It was soon recognized that planning for human exploration to Mars would impose certain unprecedent challenges, both fiscal and technical, to the current space communications paradigm. Targeting the assumed missions concepts, i.e., Crewed Mission to Phobos (CMTP) and Mars Short Stay Mission (MSSM), a Mars Planetary Network for the human exploration era has been formulated. The activity modeling and network traffic simulation/modeling we performed gave some insight into the technical challenges in space communications for the envisioned human Mars exploration era. Chief among the potential challenges are: (1) the high demand on the deep space network (DSN) assets for achieving the high-rate links, both return and forward, from Mars farthest/farther distance (up to 2.67 AU); (2) the need for resilient, persistent communication coverage for crewed vehicles, on surface and in orbits; (3) the significant period of outage for the Mars-Earth link due to superior solar conjunction; (4) the need for on-demand, simultaneous access to the proximity link by multiple vehicles and astronauts in the exploration zone; (5) the capability of determining precise, real-time, positions of surface vehicles and astronauts by the deep space habitat and/or other tele-operations entities. Solution space to each of the above challenges has been explored and analyzed in the context of the individual problem domain and, more importantly, in conjunction with that for the other challenges. This has led to an end-to-end definition of a Mars Planetary Network that would feature: (1) the fusion of deep space Ka-band and optical communications for achieving Mars-Earth high-rate links taking advantage of the optical/RF hybrid 8m/34m antennas in DSN; (2) the integrated application of the Multiple Spacecraft Per Antenna (MSPA) technique, for return link data acquisition, and the Multiple Uplink Per Antenna (MUPA) technique, for forward link data trasnmission, to reduce the number of 34m beam-wave guide (BWG) antennas needed for the era; (3) the integration of three, arrayed, 34m beam-wave guide (BWG) antennas, to provide a high G/T aperture, with the MSPA/MUPA techniques, and a dual “trunk link”approach to cut down the needed G/T -- hence, reducing the number of 34m antennas, relative to that in the single trunk approach by 50%; (4) the deployment of two areostationary/areosynchronous Mars relay orbiters; one of them could also function as (or be served by) a notional Deep Space Habitat (DSH); (5) the opportunistic deployment of a science orbiter in a Pioneer-6 type orbit, equidistant Mars/Earth, that could also serve as an intermediary relay during the Mars superior solar conjunction perod; (6) the existence of the multi-function Mars proximity link that provides the demand-assigned, multiple access (DAMA) capability; and (7) the provision of tracking observables, by leveraging on the planned Mars orbiting and surface infrastructure, to enable the in-situ navigation for surface and orbiting vehicles. This paper provides the description of the proposed Mars Planetary Network in the human exploration era, the trade-off analysis for the various alternative architectures, and the optimal solutions to the key challenges in defining this end-to-end network.

Cheung, Kar-Ming↗

NASA NTRS2018

Spacecraft Block Scheduling for NASA’s Deep Space Network

Currently, NASA’s Deep Space Network (DSN) is responsible for uplink to, downlink from, and/or tracking of dozens of missions for space agencies across the world. The DSN scheduling process starts about four months prior to the start of the schedule week, a process in which requirements are deﬁned and then the schedule is created, de-conﬂicted, and negotiated over the next 2–3 weeks with a team of mission representatives. Now scheduled for late 2019, Exploration Mission1 (EM-1) will deploy upwards of 12 SmallSat missions that will be served by the DSN. This will increase the DSN’s actively serviced spacecraft by up to 30%, further increasing the diﬃculty of meeting all mission needs via the over subscribed network. To mitigate their impact on DSN scheduling, a block scheduling process is proposed for scheduling the SmallSats. Block scheduling consists of aggregating spacecraft together into larger “pseudo-spacecraft” based on geometric alignment that then follow the same process as any other DSN mission to receive segments of track time. These tracks are then decomposed into tracks for individual users based on their speciﬁc requirements. This paper describes a full novel scheduling tool set for building candidate blocks, evaluating the eﬃcacy of these blocks, and optimal and suboptimal de-blocking schemes. To demonstrate these developed tools, results from three simulations are presented: a blocking example with lunar SmallSats, blocking potential in the greater DSN spacecraft catalog, and opportunistic multiple spacecraft per aperture potential for the DSN spacecraft catalog. Block scheduling has the potential to reduce overhead and scheduling resources for the EM-1 SmallSats while also providing them with a better means to meet their mission requirements.

Bilén, Sven G.↗

NASA NTRS2020

COSMIC: Content-based Onboard Summarization to Monitor Infrequent Change

Interplanetary exploration occurs at vast distancesthat severely limit communication bandwidth to spacecraft exploringother planets. It is possible to collect much morescientific data than can ever be downlinked given current communicationcapabilities. Therefore, we are developing a systemcalled COSMIC (Content-based Onboard Summarization toMonitor Infrequent Change) that will opportunistically analyzedata onboard a Mars orbiter to alert scientists when meaningfulchanges have occurred. COSMIC will allow future spacecraftto continuously collect data to search for rare, transient phenomenasuch as fresh impacts or seasonally changing polarlandforms under a constrained downlink budget. In this paper,we describe the overall goals and architecture of COSMIC,plans to enable specific scientific studies, label acquisition toenable supervised approaches to surface landform classification,a new machine learning evaluation framework for analyzingthe trade-offs between classifier accuracy and computationalrequirements, and lessons learned about constraints that COSMICwill face operating onboard a spacecraft. In particular, wediscuss design considerations surrounding computational andstorage constraints, change detection strategies, and localizingdetected landforms of interest within a global coordinate frame.Finally, we describe challenges and open research questions thatmust be addressed prior to deploying COSMIC.

Trockman, Asher↗

NASA NTRS2021

Microwave Radiometry at Frequencies from 500 to 1400 MHz: An Emerging Technology for Earth Observations

Microwave radiometry has provided valuable spaceborne observations of Earth's geophysical properties for decades. The recent SMOS, Aquarius, and SMAP satellites have demonstrated the value of measurements at 1400 MHz for observing surface soil moisture, sea surface salinity, sea ice thickness, soil freeze/thaw state, and other geophysical variables. However, the information obtained is limited by penetration through the subsurface at 1400 MHz and by a reduced sensitivity to surface salinity in cold or wind-roughened waters. Recent airborne experiments have shown the potential of brightness temperature measurements from 500–1400 MHz to address these limitations by enabling sensing of soil moisture and sea ice thickness to greater depths, sensing of temperature deep within ice sheets, improved sensing of sea salinity in cold waters, and enhanced sensitivity to soil moisture under vegetation canopies. However, the absence of significant spectrum reserved for passive microwave measurements in the 500–1400 MHz band requires both an opportunistic sensing strategy and systems for reducing the impact of radio-frequency interference. Here, we summarize the potential advantages and applications of 500–1400 MHz microwave radiometry for Earth observation and review recent experiments and demonstrations of these concepts. We also describe the remaining questions and challenges to be addressed in advancing to future spaceborne operation of this technology along with recommendations for future research activities.

Microwave radiometry↗

NASA NTRS2021

Microwave Radiometry at Frequencies From 500 to 1400 MHz: An Emerging Technology for Earth Observations

microwave radiometry↗

NASA NTRS2022

Novel Parameterization of Ice Cloud Effective Diameter from Collocated CALIOP-IIR and CloudSat Retrievals

Satellite-based measurements of global ice cloud microphysical properties are sampled to develop a novel set of physical parameterizations, relating to cloud layer temperature and effective diameter D(e), that can be implemented for two separate applications: in numerical weather prediction models and lidar-based cloud radiative forcing studies. Ice cloud optical properties (i.e., spectral scattering and absorption) are estimated based on the effective size and habit mixture of the cloud particles. Historically, the ice cloud D(e) has been parameterized from aircraft in situ measurements. However, aircraft-based parameterizations are opportunistic in that they only represent specific types of clouds (e.g., convective anvil, tropopause-topped cirrus) in the regions in which they were sampled and, in some cases, are limited in fully resolving the entire vertical cloud layer. Breaking away from the aircraft-based parameterization paradigm, this study is the first of its kind to attempt a parameterization of D(e) as a function of temperature, ice water content (IWC), and lidar-derived extinction from satellite-based global oceanic measurements of ice clouds. Data from both active and passive remote sensing sensors from two of NASA’s A-Train satellites, CloudSat and CALIPSO, are collected to guide development of globally robust parameterizations of all ice cloud types and one exclusively for cirrus clouds.

Ice Cloud↗

NASA NTRSDate not supplied

SPACEFLIGHT-INDUCED CHANGES IN MICROBIAL VIRULENCE AND THE IMPACT TO THE HOST IMMUNE RESPONSE

INTRODUCTION Over the past 50 years, microorganisms have displayed unexpected responses relevant to infectious disease when grown in microgravity and microgravity analogue environments, including changes in final cell concentration, biofilm production, stress resistance, antibiotic sensitivity, gene expression, and virulence. In parallel, astronaut studies have characterized a persistent spaceflight-induced dysregulation of the human immune system; consisting of altered leukocyte distribution, reductions in T and NK cell function, altered cytokine profiles, and reactivation of latent herpesviruses. Further, astronauts have some degree of clinical incidence, primarily infectious disease episodes and atopic dermatitis. The impact of the microgravity environment on host-pathogen interactions and potential for clinical disease remains understudied and poorly characterized. SPECIFIC AIMS In the Host-Microorganism, Microbial Response aspect of this study, the following Specific Aims are being investigated, using the microbial pathogens, Salmonella enterica Enteritidis, Pseudomonas aeruginosa, Burkholderia cepacia, Streptococcus pneumoniae, and enterohemorrhagic Escherichia coli. Aim 1: Characterize the effect of spaceflight analogue culture on microbial pathogenesis-related stress responses and in vitro host-pathogen interactions. Analysis will include microbial stress responses as well as colonization and viability following pathogen challenge of three-dimensional (3-D) tissue co-culture models containing immune cells. We specifically selected obligate and opportunistic pathogens that are medically important and have been or are likely to be found aboard spacecraft. Aim 2: Characterize the effect of spaceflight analogue culture on the virulence potential of pathogenic microorganisms. Changes in virulence will be assessed using a mouse model of infection. SIGNIFICANCE The goal of this study is to use spaceflight analogue conditions to gain insight into the breadth of medically-significant International Space Station microorganisms that have altered virulence and the impact of those changes on the immune response of the host, including astronaut immune cells. This information will provide critical understanding into the impact of microgravity on potential alterations in microbial virulence and associated infectious disease risk to crew health during spaceflight missions.

C. M. Ott↗

NASA NTRS2022

The Future: Nasa’s Surface Biology and Geology Mission and the Dark (Aquatic) Side

With the release of NASA’s Earth System Observatory (ESO) measurement targets that includes studying the Earth’s Surface Biology and Geology (SBG), a global visible to shortwave infrared imaging spectrometer and a multispectral thermal infrared imager have been planned to launch in the late 2020s. This mission will enable unprecedented interdisciplinary science and applications relevant to studying the biology and geology of the Earth's surface. Measurement targets are directly aligned for studying coastal and inland waters ecosystems and water quality, including coralreef ecosystems, snow and ice, mineralogy, volcanology, biology, ecology, biodiversity, and components of radiative forcing from the surface such as greenhouse gas emissions. The observations not only have scientific value in studying feedbacks and interactions of surface processes (e.g., harmful algal blooms, domoic acid, oil spills, and other hazardous events), but also societal benefit – with the capacity to support real-world decision-making such as response to theseevents and inland water conservation, protection of drinking water quality for public and environmental health, and habitat conservation. The work presented here outlines the conducted mission and architecture study and the initiation of science performance trades (signal sensitivity; spectral, spatial, and temporal resolution; and atmospheric effects) informing the design of the SBG segment of the ESO providing the most value to myriad science and applications as possible. Science and applications synergies and algorithms with the Plankton, Aerosol, Cloud, and ocean Ecosystem (PACE) and the Geostationary Littoral Imaging and Monitoring Radiometer (GLIMR) is opportunistic for advancing aquatics research and applications.

THE FUTURE↗

NASA NTRSDate not supplied

Effects of Spaceflight Relevant Carbon Dioxide Levels on Pathogenesis Related Microbial Characteristics

BACKGROUND Multiple stressors in the spaceflight environment have the potential to alter microbial pathogenesis and virulence characteristics, including microgravity, radiation, and hostile/confined conditions. This combination of factors may lead to a stacking of risks, potentially creating unexpected increase in the risk of infectious disease. One understudied variable is the higher CO2 atmospheric concentration. The current Spaceflight Maximum Allowance Concentration (SMAC) for 24-hour average CO2 is 0.4% (3 mm Hg). These elevated ambient CO2 levels aboard the ISS could potentially influence the diversity and phenotypic responses of the resident microbial communities from both the spacecraft environment (air, surface, water) and crewmembers (gut, nasal, skin microbiomes). METHODS For these studies, we will evaluate the response of Staphylococcus aureus, Streptococcus pneumoniae and Enterobacter aerogenes, which are opportunistic pathogens that are of medical significance and have been or are likely to be found aboard spacecraft. These microorganisms will be cultured in both spaceflight analog and control conditions at CO2levels representing terrestrial atmospheric concentration of 0.04% (0.3 mmHg), ISS elevated concentration of 0.4% (3 mm Hg), and an unexpected elevated concentration of 1 % (7.6 mmHg) to represent a worst case scenario. DISCUSSION Overwhelming evidence from studies performed in food microbiology, marine biology and terrestrial environmental biology suggest altered gene expression, selective bacterial inhibition, increased growth and diversity, and increased antibiotic resistance of bacterial communities individually and in biofilm formation when exposed to increased levels of CO2. While many of these findings are based on levels of CO2 higher than would be found during spaceflight exploration missions, the potential for subtle changes in CO2 levels to exacerbate infectious disease risks warrants an evaluation of pathogen responses to spaceflight CO2 conditions. Our study will provide answers to spaceflight relevant atmospheric conditions, as described in the Risk of Adverse Health Effects Due to Host-Microorganism Interaction (GapMicro 103).

A A Medina-Colorado↗