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At least 127 records · Page 7

A CLC-ec1 mutant reveals global conformational change and suggests a unifying mechanism for the CLC Cl–/H+ transport cycle

Among coupled exchangers, CLCs uniquely catalyze the exchange of oppositely charged ions (Cl– for H+). Transport-cycle models to describe and explain this unusual mechanism have been proposed based on known CLC structures. While the proposed models harmonize with many experimental findings, gaps and inconsistencies in our understanding have remained. One limitation has been that global conformational change – which occurs in all conventional transporter mechanisms – has not been observed in any high-resolution structure. Here, we describe the 2.6 Å structure of a CLC mutant designed to mimic the fully H+-loaded transporter. This structure reveals a global conformational change to improve accessibility for the Cl– substrate from the extracellular side and new conformations for two key glutamate residues. Together with DEER measurements, MD simulations, and functional studies, this new structure provides evidence for a unified model of H+/Cl– transport that reconciles existing data on all CLC-type proteins.

59 BASIC BIOLOGICAL SCIENCES↗

A release mechanism with mechanical redundancy

A simple release mechanism is described that achieves high reliability through active mechanical redundancy on the SERT 2 satellite. Principle of operation, design details, and the test program are discussed. Examples of developmental and proposed alternate configurations also are included.

Paradise, J. J.↗

Reliability breakthrough: An antenna deployment/positioning mechanism with electrical and mechanical redundancy

An Antenna Positioner Mechanism (APM) was developed for deployment of an antenna reflector and for fine granularity closed loop tracking of the antenna in response to RF beacon error signals. By utilizing permanent magnet stepper motors, spur gearheads, irreversible single threat worm/wheel assemblies and a miter gear differential, full electrical and mechanical redundancy was realized. Two versions of this design were generated: one is a weight-optimized design with a clutch for overload protection and one is a more rugged unit without a clutch.

Olson, M. C.↗

Fluid mechanics mechanisms in the stall process of helicopters

Recent experimental results from airfoils in the Mach number, Reynolds number, or reduced frequency ranges typical of helicopter rotor blades have identified the most influential flow mechanisms in the dynamic stall process. The importance of secondary shed vortices, downstream wake action, and the flow in the separated region is generally acknowledged but poorly understood. By means of surface pressure cross-correlations and flow field measurements in static stall, several new hypotheses have been generated. It is proposed that vortex shedding may be caused by acoustic disturbances propagating forward in the lower (pressure) surface boundary layer, that wake closure is a misnomer, and that the shed vortex leaves a trail of vorticity that forms a turbulent free shear layer. The known dynamic stall flow mechanisms are reviewed and the potential importance of recently proposed and hypothetical flow phenomena with respect to helicopter blade aeroelastic response are assessed.

Young, W. H., Jr.↗

Computational mechanics - Advances and trends; Proceedings of the Session - Future directions of Computational Mechanics of the ASME Winter Annual Meeting, Anaheim, CA, Dec. 7-12, 1986

The papers contained in this volume provide an overview of the advances made in a number of aspects of computational mechanics, identify some of the anticipated industry needs in this area, discuss the opportunities provided by new hardware and parallel algorithms, and outline some of the current government programs in computational mechanics. Papers are included on advances and trends in parallel algorithms, supercomputers for engineering analysis, material modeling in nonlinear finite-element analysis, the Navier-Stokes computer, and future finite-element software systems.

Noor, Ahmed K.↗

Examination of the discharge mechanism of Li/CF(x) cells: Comparison of the electrochemical reduction mechanisms of PTFE and (CF(x))n by lithium

Swelling of (CF(x))(sub n) electrodes in commercial Li/(CF(x))(sub n) cells presents a limiting factor in cell design optimization. Examination of cathodes from such cells, after discharge, reveals a relation between cell operating temperatures and cathode swelling. Attempts to explain the swelling using the prevailing model for the cathode reaction have failed. A more suitable reaction mechanism is proposed based on the observed behavior of (CF(x))(sub n) electrodes on discharge and a comparison of the reaction products of (CF(x))(sub n) and polytetra fluoroethylene (PTFE) with lithium amalgams. The proposed mechanism is in agreement with the experimental data found in the literature.

Baxam, Carl C.↗

Prestressing Shock Resistant Mechanical Components and Mechanisms Made from Hard, Superelastic Materials

A method and an apparatus confer full superelastic properties to the active surface of a mechanical component constructed of a superelastic material prior to service. A compressive load is applied to the active surface of the mechanical component followed by removing the compressive load from the active surface whereby substantially all load strain is recoverable after applying and removing of subsequent compressive loads.

DellaCorte, Christopher↗

Mechanical design and stress analysis challenges overcome to ensure the structural integrity of Europa Clipper's mechanical pumped fluid loop Heat Redistribution System (HRS)

Launching in 2023, NASA’s Europa Clipper mission will place a spacecraft into a long, looping orbit around Jupiter to perform a series of close flyby investigations of its moon Europa. Strong evidence suggests that Europa hosts an ocean of liquid water beneath its icy crust that could harbor conditions favorable for life. The spacecraft is currently under development by the Jet Propulsion Laboratory (JPL) and the Applied Physics Laboratory (APL) and nearing completion of its Detailed Design Phase. A Mechanical Pumped Fluid Loop (MPFL) architecture known as the Heat Redistribution System (HRS) has been baselined to provide power efficient thermal control of the sensitive components within the Avionics, Radio Frequency (RF), and Propulsion Modules. Principally, the HRS harvests waste heat from the onboard dissipating equipment and distributes it to a 1.4 m diameter by 3 m tall cylindrical structure that cocoons and keeps the propulsion subsystem warm. Numerous technical issues had to be resolved in order to securely attach nearly 100 m of HRS tubing to the spacecraft, which is predicted to experience high launch loads. This paper will review the relevant environments and mission constraints as well as outline the stress and fatigue analysis approach taken to verify the detailed mechanical design of the HRS.

Kolenz, Daniel↗

Systems for mechanical logic based on additively manufacturable micro-mechanical logic gates

The present disclosure is directed to various mechanical logic gates. In one example a mechanical logic NOT gate system is formed which has a first pair of bi-stable buckling structures each being operatively connected at a first connection point thereof to one another, and also to a first rigid structure at second connection points, the first rigid structure being held stationary. A second pair of bi-stable buckling flexures is each operatively connected at first connection points thereof to each other and at second connection points thereof to a second rigid structure being held stationary. An output element is coupled a first one of each of the first and second pairs of bi-stable buckling structures. An input element is coupled to a second one of each of the first and second pairs of bi-stable buckling structures. The output element moves from a logic 1 position to a logic 0 position in response to movement of the input element from a logic 0 position to a logic 1 positions, respectively.

42 ENGINEERING↗

Metallic components with enhanced mechanical strength through surface mechanical grinding

A method of strengthening a component made of a metallic material. The method includes subjecting the component to a mechanical grinding process incorporating a relative motion between a tool and the component forming a gradient structure on the surface of the component, resulting in increased tensile strength of the component. A method of strengthening a component made of a TWIP steel. The method includes subjecting the component made of TWIP steel to a mechanical grinding process incorporating a relative motion between a tool and the component forming a gradient structure containing a surface nanolaminate layer, a shear band layer, and an inner deformation twinned layer, resulting in increased tensile strength of the component. A component made of a TWIP steel containing a gradient structure with a surface nanolaminate layer, a shear band layer, and a deformation twinned layer.

Ding, Jie↗

Metallic components with enhanced mechanical strength through surface mechanical grinding

A method of strengthening a component made of a metallic material. The method includes subjecting the component to a mechanical grinding process incorporating a relative motion between a tool and the component forming a gradient structure on the surface of the component, resulting in increased tensile strength of the component. A method of strengthening a component made of a TWIP steel. The method includes subjecting the component made of TWIP steel to a mechanical grinding process incorporating a relative motion between a tool and the component forming a gradient structure containing a surface nanolaminate layer, a shear band layer, and an inner deformation twinned layer, resulting in increased tensile strength of the component. A component made of a TWIP steel containing a gradient structure with a surface nanolaminate layer, a shear band layer, and a deformation twinned layer.

Ding, Jie↗

Molecular Dynamical and Quantum Mechanical Exploration of the Site-Specific Dynamics of Cy3 Dimers Internally Linked to dsDNA

Performing spectroscopic measurements on biomolecules labeled with fluorescent probes is a powerful approach to locating the molecular behavior and dynamics of large systems at specific sites within their local environments. The indocarbocyanine dye Cy3 has emerged as one of the most commonly used chromophores. The incorporation of Cy3 dimers into DNA enhances experimental resolution owing to the spectral characteristics influenced by the geometric orientation of excitonically coupled monomeric units. Various theoretical models and simulations have been utilized to aid in the interpretation of the experimental spectra. In this study, we employ all-atom molecular dynamics simulations to study the structural dynamics of Cy3 dimers internally linked to the dsDNA backbone. We used quantum mechanical calculations to derive insights from both the linear absorption spectra and the circular dichroism data. Furthermore, we explore potential limitations within a commonly used force field for cyanine dyes. The molecular dynamics simulations suggest the presence of four possible Cy3 dimeric populations. The spectral simulations on the four populations show one of them to agree better with the experimental signatures, suggesting it to be the dominant population. Furthermore, the relative orientation of Cy3 in this population compares very well with previous predictions from the Holstein–Frenkel Hamiltonian model.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

In–Depth Analysis of the Degradation Mechanisms of High–Nickel, Low/No–Cobalt Layered Oxide Cathodes for Lithium–Ion Batteries

A rational compositional design of high-nickel, cobalt-free layered oxide materials for high-energy and low-cost lithium-ion batteries would be expected to further propel the widespread adoption of electric vehicles (EVs), yet a composition with satisfactory electrochemical properties has yet to emerge. The previous work has demonstrated a promising LiNi 0.883 Mn 0.056 Al 0.061 O 2 (NMA-89) composition that outperformed high-nickel, cobalt-containing analogs in cycling stability and maintained a comparable rate performance and thermal stability. Herein, the capacity fading mechanism of NMA-89 in a pouch full cell with a 4.2 V cutoff is compared to that of its cobalt-containing analogs. The results reveal that particle cracking in LiNi 0.89 Mn 0.055 Co 0.055 O 2 (NMC-89) and LiNi 0.883 Co 0.053 Al 0.064 O 2 (NCA-89) leads to a loss of active material and an increase in surface area, thereby exacerbating structural and surface instabilities, accelerating impedance and polarization growth, and ultimately reducing their capacity retentions. LiNi 0.89 Mn 0.044 Co 0.042 Al 0.013 Mg 0.011 O 2 (NMCAM-89) and NMA-89 experience subdued surface reactions and maintain spherical particle structures, both of which are conducive to their capacity retentions during long-term cycling. Furthermore, this investigation offers insights into how specific transition-metal ions dictate the electrochemical stability of high-Ni layered oxide cathode materials, highlights the benefit of Mn-Al combination in NMA-89, and presents potential strategies to further enhance the performance of this novel class of cathode materials.

degradation mechanisms↗