Molecular origins of reduced activity and binding commitment of processive cellulases and associated carbohydrate-binding proteins to cellulose III
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59 BASIC BIOLOGICAL SCIENCES↗
SEARCH · Engineering Papers
Results for “carbohydrate”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Carbohydrate-binding module O -mannosylation alters binding selectivity to cellulose and lignin
Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Carbohydrates great and small, from dietary fiber to sialic acids: How glycans influence the gut microbiome and affect human health
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59 BASIC BIOLOGICAL SCIENCES↗
Techno-Economic Analysis of Biofuel Production via Catalytic Upgrading of Carbohydrates in Paper Sludge
A full-plant model was developed using correlations from a rigorous mass and energy balance and based on experimental results to determine the MFSP and its Sensitivity Analysis in the production of a hydrocarbon product from Paper Sludge. In this base case a preliminary MFSP of $5.97/GGE has been determined. This value is higher than the results reported in recent NREL reports. The difference is explained since much larger capacity plants are considered in those reports, and because we are not considering the production of co-products that bring the fuel costs down. The obtained model is being used to guide the next steps in our laboratory work since the team is now aware of which are the more sensitive process parameters "impacting" the MFSP of the final product.
29 ENERGY PLANNING, POLICY, AND ECONOMY↗
Helicobacter pylori lipopolysaccharide structural domains and their recognition by immune proteins revealed with carbohydrate microarrays
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Silva, Lisete M. (ORCID:0000000348765240)↗
Distinctive carbon repression effects in the carbohydrate-selective wood decay fungus Rhodonia placenta
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59 BASIC BIOLOGICAL SCIENCES↗
Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain
Explore the source record for details and available documents.
99 GENERAL AND MISCELLANEOUS↗
Carbohydrate-aromatic interface and molecular architecture of lignocellulose
Abstract Plant cell walls constitute the majority of lignocellulosic biomass and serve as a renewable resource of biomaterials and biofuel. Extensive interactions between polysaccharides and the aromatic polymer lignin make lignocellulose recalcitrant to enzymatic hydrolysis, but this polymer network remains poorly understood. Here we interrogate the nanoscale assembly of lignocellulosic components in plant stems using solid-state nuclear magnetic resonance and dynamic nuclear polarization approaches. We show that the extent of glycan-aromatic association increases sequentially across grasses, hardwoods, and softwoods. Lignin principally packs with the xylan in a non-flat conformation via non-covalent interactions and partially binds the junction of flat-ribbon xylan and cellulose surface as a secondary site. All molecules are homogeneously mixed in softwoods; this unique feature enables water retention even around the hydrophobic aromatics. These findings unveil the principles of polymer interactions underlying the heterogeneous architecture of lignocellulose, which may guide the rational design of more digestible plants and more efficient biomass-conversion pathways.
59 BASIC BIOLOGICAL SCIENCES↗
The energetic landscape of CH–π interactions in protein–carbohydrate binding
Over hundreds of PDB structures of protein–sugar complexes, our work demonstrates the prevalence of CH–π stacking interactions, and energetic analysis shows that numerous orientations allow for highly favorable interaction strengths.
Keys, Allison M. [Computational and Systems Biolog↗
A sweet new study: tropical forest species use nonstructural carbohydrates in different ways during drought
Not Available
Powers, Jennifer S.↗
Diverse modes of galacto-specific carbohydrate recognition by a family 31 glycoside hydrolase from Clostridium perfringens
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99 GENERAL AND MISCELLANEOUS↗
Species-Specific Duplication Event Associated with Elevated Levels of Nonstructural Carbohydrates in Sorghum bicolor
Simple sugars are the essential foundation to plant life, and thus, their production, utilization, and storage are highly regulated processes with many complex genetic controls. Despite their importance, many of the genetic and biochemical mechanisms remain unknown or uncharacterized. Sorghum, a highly productive, diverse C4 grass important for both industrial and subsistence agricultural systems, has considerable phenotypic diversity in the accumulation of nonstructural sugars in the stem. We use this crop species to examine the genetic controls of high levels of sugar accumulation, identify genetic mechanisms for the accumulation of nonstructural sugars, and link carbon allocation with iron transport. We identify a species-specific tandem duplication event controlling sugar accumulation using genome-wide association analysis, characterize multiple allelic variants causing increased sugar content, and provide further evidence of a putative neofunctionalization event conferring adaptability in Sorghum bicolor. Comparative genomics indicate that this event is unique to sorghum which may further elucidate evolutionary mechanisms for adaptation and divergence within the Poaceae. Furthermore, the identification and characterization of this event was only possible with the continued advancement and improvement of the reference genome. The characterization of this region and the process in which it was discovered serve as a reminder that any reference genome is imperfect and is in need of continual improvement.
59 BASIC BIOLOGICAL SCIENCES↗