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At least 127 records · Page 7

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NASA NTRS2005

BEAM Technology Flight Demonstration

As technologies advance, their growing complexity makes them harder to maintain. Detection methods for isolating and identifying impending problems are needed to balance this complexity. Through comparison of signal pairs from onboard sensors, the Beacon-based Exception Analysis For Multimissions (BEAM) algorithm can identify and help classify deviations in system operation from a data-trained statistical model. The goal of this task is to mature BEAM and validate its performance on a flying test bed. A series of F-18 flight demonstrations with BEAM monitoring engine parameters in real time was used to demonstrate in-the-field readiness. Captured F-18 and simulated F-18 engine data were used in model creation and training. The algorithm was then ported to the embedded system with a data buffering, file writing, and data-time-stamp monitoring shell to reduce the impact of embedded system faults on BEAM'S ability to correctly identify engine faults. Embedded system testing identified hardware related restrictions and contributed to iterative improvements in the code's runtime performance. The system was flown with forced engine flameouts and other pilot induced faults to simulate operation out of the norm. Successful detection of these faults, confirmed through post-flight data analysis, helped BEAM achieve TRL6.

Wang, David↗

NASA NTRS1986

Electrophoretic separation and analysis of living cells from solid tissues by several methods - Human embryonic kidney cell cultures as a model

The use of free-fluid electrophoresis methods to separate tissue cells having a specific function is discussed. It is shown that cells suspended by trypsinization from cultures of human embryonic kidney are electrophoretically heterogeneous and tolerate a wide range of electrophoresis buffers and conditions without significant attenuation of function. Moreover, these cells do not separate electrophoretically on the basis of size or cell position alone and can be separated according to their ability to give rise to progeny that produce specific plasminogen activators.

Todd, Paul↗

NASA NTRS1984

The role of the reflection coefficient in precision measurement of ultrasonic attenuation

Ultrasonic attenuation measurements using contact, pulse-echo techniques are sensitive to surface roughness and couplant thickness variations. This can reduce considerable inaccuracies in the measurement of the attenuation coefficient for broadband pulses. Inaccuracies arise from variations in the reflection coefficient at the buffer-couplant-sample interface. The reflection coefficient is examined as a function of the surface roughness and corresponding couplant thickness variations. Interrelations with ultrasonic frequency are illustrated. Reliable attenuation measurements are obtained only when the frequency dependence of the reflection coefficient is incorporated in signal analysis. Data are given for nickel 200 samples and a silicon nitride ceramic bar having surface roughness variations in the 0.3 to 3.0 microns range for signal bandwidths in the 50 to 100 MHz range.

Generazio, E. R.↗

NASA NTRS1985

The role of the reflection coefficient in precision measurement of ultrasonic attenuation

Generazio, E. R.↗

NASA NTRS1979

Thermal analysis of turbulent flow of a supercritical fluid

The influence of the large variation of thermodynamics and transport properties near the pseudocritical temperature on the heat transfer coefficient of supercritical fluid in turbulent flow was studied. The formation of the characteristics peak in the heat transfer coefficient vs. bulk temperature curve is described, and the necessity of the fluid element at pseudocritical temperature located in the buffer layer is discussed.

Yamane, E.↗

NASA NTRS1982

The role of multispectral scanners as data sources for EPA hydrologic models

An estimated cost savings of 30% to 50% was realized from using LANDSAT-derived data as input into a program which simulates hydrologic and water quality processes in natural and man-made water systems. Data from the satellite were used in conjunction with EPA's 11-channel multispectral scanner to obtain maps for characterizing the distribution of turbidity plumes in Flathead Lake and to predict the effect of increasing urbanization in Montana's Flathead River Basin on the lake's trophic state. Multispectral data are also being studied as a possible source of the parameters needed to model the buffering capability of lakes in an effort to evaluate the effect of acid rain in the Adirondacks. Water quality in Lake Champlain, Vermont is being classified using data from the LANDSAT and the EPA MSS. Both contact-sensed and MSS data are being used with multivariate statistical analysis to classify the trophic status of 145 lakes in Illinois and to identify water sampling sites in Appalachicola Bay where contaminants threaten Florida's shellfish.

Slack, R.↗

NASA NTRS1989

Performance evaluation of automated manufacturing systems using generalized stochastic Petri Nets

The main objective of this research is to develop a generic modeling methodology with a flexible and modular framework to aid in the design and performance evaluation of integrated manufacturing systems using a unified model. After a thorough examination of the available modeling methods, the Petri Net approach was adopted. The concurrent and asynchronous nature of manufacturing systems are easily captured by Petri Net models. Three basic modules were developed: machine, buffer, and Decision Making Unit. The machine and buffer modules are used for modeling transfer lines and production networks. The Decision Making Unit models the functions of a computer node in a complex Decision Making Unit Architecture. The underlying model is a Generalized Stochastic Petri Net (GSPN) that can be used for performance evaluation and structural analysis. GSPN's were chosen because they help manage the complexity of modeling large manufacturing systems. There is no need to enumerate all the possible states of the Markov Chain since they are automatically generated from the GSPN model.

Al-Jaar, Robert Y.↗

NASA NTRS2002

Parametric Study of Carbon Nanotube Production by Laser Ablation Process

Carbon nanotubes form a new class of nanomaterials that are presumed to have extraordinary mechanical, electrical and thermal properties. The single wall nanotubes (SWNTs) are estimated to be 100 times stronger than steel with 1/6th the weight; electrical carrying capacity better than copper and thermal conductivity better than diamond. Applications of these SWNTs include possible weight reduction of aerospace structures, multifunctional materials, nanosensors and nanoelectronics. Double pulsed laser vaporization process produces SWNTs with the highest percentage of nanotubes in the output material. The normal operating conditions include a green laser pulse closely followed by an infrared laser pulse. Lasers ab late a metal-containing graphite target located in a flow tube maintained in an oven at 1473K with argon flow of 100 sccm at a 500 Torr pressure. In the present work a number of production runs were carried out, changing one operating condition at a time. We have studied the effects of nine parameters, including the sequencing of the laser pulses, pulse separation times, laser energy densities, the type of buffer gas used, oven temperature, operating pressure, flow rate and inner flow tube diameters. All runs were done using the same graphite target. The collected nanotube material was characterized by a variety of analytical techniques including scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman and thermo gravimetric analysis (TGA). Results indicate trends that could be used to optimize the process and increase the efficiency of the production process.

Arepalli, Sivaram↗

NASA NTRS1991

Survival of epiphytic bacteria from seed stored on the Long Duration Exposure Facility (LDEF)

This study was designed to determine the survival of microorganisms exposed to the relatively harsh conditions found in low Earth orbit (LEO). Seed of corn, sunflower, canteloupe, zucchini, bean, pea, and pumpkin cultivars were packaged in two 18 x 2.5 cm aluminum tubes; wall thickness for each tube was 1.33 mm. One seed tube was attacked to payload M0006, tray C-2; a second tube was stored at room temperature in a lab on Earth. Five lithium fluoride thermoluminescent dosimetry wafers (TLD-100 wafers) were placed in each aluminum tube. The total mean dosages for flight and ground-control TLD wafers were 210.0 and 0.9 rads, respectively. Seeds were washed for 2 hrs in a phosphate buffered saline solution. Bacteria were isolated by plating samples of the seed-washings onto dilute tryptic soy agar. Pure isolates of morphologically distinct bacteria were obtained by standard microbiological procedures. Bacteria were grouped according to colony-type and preliminary identification was completed using a fatty-acid analysis system. Bacillus spp. were the primary microoganisms that survived on seed during the experiment. Bacterial diversity and relative abundance were similar for the ground flight seed. Bacillus subtilus, B. pumilus, B. licheniformis, B. polymyxa, B. megaterium, and B. pabuli were isolated most frequently. Members of the genera Kurthia, Listeria, Micrococcus, and Arthrobacter were also isolated from flight and ground control seed. Results support the hypothesis that terrestrial microorganisms can survive long periods of time in the relatively harsh LEO environment.

Schuerger, Andrew C.↗

NASA NTRS1998

Effects of Microheterogeneity in Hen Egg-White Lysozyme Crystallization

In earlier sodium dodecylsulfate polyacylamide gel electrophoresis (SDS-PAGE) studies it has been found that commonly utilized commercial hen egg-white lysozyme (HEWL) preparations contained 0.2-0.4 mol% covalently bound dimers. Here it is shown, using high-performance capillary electrophoresis (HPCE), that HEWL contains, in addition, two differently charged monomers in comparable amounts. To explore the origin of these microheterogeneous contaminants, purified HEWL (PHEWL) has been oxidized with hydrogen peroxide (0.0026-0.88 M) at various pH levels between 4.5 and 12.0. Optical densitometry of oxidized PHEWL (OHEWL) bands in SDS PAGE gels shows that hydrogen peroxide at 0.88 M in acetate buffer pH 4.5 increased the amount of dimers about sixfold over that in commercial HEWL. OHEWL had, in addition to one of the two monomer forms found in HEWL and PHEWL, three other differently charged monomer forms, each of them representing about 25% of the preparation. SDS-PAGE analysis of OHEWL yielded two closely spaced dimer bands with M(sub r) = 28 000 and 27 500. In addition, larger HEWL oligomers with M, = 1.7 million and 320 000 were detected by gel-filtration fast protein liquid chromatography with multiangle laser light scattering detection. Non-dissociating PAGE in large pore size gels at pH 4.5 confirmed the presence of these large oligomers in HEWL and OHEWL. Increased microheterogeneity resulted in substantial effects on crystal growth and nucleation rate. On addition of 10 microgram(exp -1) mg ml(exp -1) OHEWL to 32 mg ml(exp -1) HEWL crystallizing solutions, both the number and size of forming crystals decreased roughly proportionally to the concentration of the added microheterogeneity. The same effect was observed in HEWL solutions on addition of 0.03-9,3 M Hydrogen peroxide. Repartioning of the dimer during crystallzation aat various temperatures between 277 and 293 K was analyzed by SDS-PAGE. The crystals contained <= 25 % weight by volume of the oligomers in the solution, with no apparent temperature dependence of the repartioning.

Thomas, B. R.↗

NASA NTRS2019

Rising Above the Cloud - Toward High-Rate Delay-Tolerant Networking in Low-Earth Orbit

The High Data Rate Architecture (HiDRA) project is implementing a High-rate Delay Tolerant Networking (HDTN) capability that can support Low Earth Orbit (LEO) applications and environments. The present state of the effort, future work, and other elements of the work to date are described in this paper. This implementation is intended to support applications that run at 1+ Gbps, per the requirements of modern optical and high-frequency RF links. Uniquely, this implementation is also tuned to support relay and data trunking applications, which might require support for large numbers of small bundles per second. The design for this platform is based entirely on commercial-off-the-shelf (COTS) components, and possesses buffering capabilities in the 5 TB range. This document takes results from previous individual tests and integrates them to demonstrate results in the presence of a coherent use-case: consider a network aboard the ISS which intends to utilize an upcoming optical communications capability. For this use-case, orbital analysis software is used to analyze orbital dynamics, from which a list of access times are generated that might take in to account weather, schedule competition, etc. A variant of Contact Graph Routing (CGR) is applied to these windows to determine an optimal schedule. This schedule is then loaded into the HDTN prototype and, in conjunction with various measurement tools, a complete end-to-end analysis of HDTN's performance is conducted. Various bottlenecks (including storage) are identified: these bottlenecks are expected to help us focus our future work on the elements of the system that are most likely to present issues moving forward. Finally, we discuss possible paths for evolution beyond the present rates supported by the system, including (but not limited to) hardware acceleration.

Hylton, Alan↗

NASA NTRS2014

Ten Years of Forest Cover Change in the Sierra Nevada Detected Using Landsat Satellite Image Analysis

The Landsat Ecosystem Disturbance Adaptive Processing System (LEDAPS) methodology was applied to detected changes in forest vegetation cover for areas burned by wildfires in the Sierra Nevada Mountains of California between the periods of 1975- 79 and 1995-1999. Results for areas burned by wildfire between 1995 and 1999 confirmed the importance of regrowing forest vegetation over 17% of the combined burned areas. A notable fraction (12%) of the entire 5-km (unburned) buffer area outside the 1995-199 fires perimeters showed decline in forest cover, and not nearly as many regrowing forest areas, covering only 3% of all the 1995-1999 buffer areas combined. Areas burned by wildfire between 1975 and 1979 confirmed the importance of disturbed (or declining evergreen) vegetation covering 13% of the combined 1975- 1979 burned areas. Based on comparison of these results to ground-based survey data, the LEDAPS methodology should be capable of fulfilling much of the need for consistent, low-cost monitoring of changes due to climate and biological factors in western forest regrowth following stand-replacing disturbances.

Vegetation↗

NASA NTRS1992

Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape

Studies were carried out with capillary endothelial cells cultured on fibronectin (FN)-coated dishes in order to analyze the mechanism of cell and nuclear shape control by extracellular matrix (ECM). To examine the role of the cytoskeleton in shape determination independent of changes in transmembrane osmotic pressure, membranes of adherent cells were permeabilized with saponin (25 micrograms/ml) using a buffer that maintains the functional integrity of contractile microfilaments. Real-time videomicroscopic studies revealed that addition of 250 microM ATP resulted in time-dependent retraction and rounding of permeabilized cells and nuclei in a manner similar to that observed in intact living cells following detachment using trypsin-EDTA. Computerized image analysis confirmed that permeabilized cells remained essentially rigid in the absence of ATP and that retraction was stimulated in a dose-dependent manner as the concentration of ATP was raised from 10 to 250 microM. Maximal rounding occurred by 30 min with projected cell and nuclear areas being reduced by 69 and 41%, respectively. ATP-induced rounding was also accompanied by a redistribution of microfilaments resulting in formation of a dense net of F-actin surrounding retracted nuclei. Importantly, ATP-stimulated changes in cell, cytoskeletal, and nuclear form were prevented in permeabilized cells using a synthetic myosin peptide (IRICRKG) that has been previously shown to inhibit actomyosin filament sliding in muscle. In contrast, both the rate and extent of cell and nuclear rounding were increased in permeabilized cells exposed to ATP when the soluble FN peptide, GRGDSP, was used to dislodge immobilized FN from cell surface integrin receptors.(ABSTRACT TRUNCATED AT 250 WORDS).

Non-NASA Center↗

NASA NTRS2020

Detect-and-Avoid: Flight Test 6 Scripted Encounters Data Analysis

The Unmanned Aircraft System (UAS) in the National Airspace System (NAS) project conducted Flight Test 6 (FT6) in 2019. The ultimate goal of this flight test was to produce data to inform RTCA SC-228's Phase II Minimum Operational Performance Standards (MOPS) for Detect and Avoid (DAA) and Low Size, Weight, and Power Sensors. This report documents the analysis of scripted encounters' data. Scripted encounters own were analyzed and categorized based on the outcome of alert, maneuver guidance, and effectiveness of pilots' maneuver in resolving conflicts. Results indicate that UAS pilots' decisions as well as intruder maneuvers are leading factors that contribute to ineffective DAA maneuvers. Results also show that adding buffers to the DAA's suggested minimum turn angle improves effectiveness of the DAA maneuvers.

Wang, Wei-Ching↗

NASA NTRS1996

Fundamental relations between short-term RR interval and arterial pressure oscillations in humans

BACKGROUND: One of the principal explanations for respiratory sinus arrhythmia is that it reflects arterial baroreflex buffering of respiration-induced arterial pressure fluctuations. If this explanation is correct, then elimination of RR interval fluctuations should increase respiratory arterial pressure fluctuations. METHODS AND RESULTS: We measured RR interval and arterial pressure fluctuations during normal sinus rhythm and fixed-rate atrial pacing at 17.2+/-1.8 (SEM) beats per minute greater than the sinus rate in 16 healthy men and 4 healthy women, 20 to 34 years of age. Measurements were made during controlled-frequency breathing (15 breaths per minute or 0.25 Hz) with subjects in the supine and 40 degree head-up tilt positions. We characterized RR interval and arterial pressure variabilities in low-frequency (0.05 to 0.15 Hz) and respiratory-frequency (0.20 to 0.30 Hz) ranges with fast Fourier transform power spectra and used cross-spectral analysis to determine the phase relation between the two signals. As expected, cardiac pacing eliminated beat-to-beat RR interval variability. Against expectations, however, cardiac pacing in the supine position significantly reduced arterial pressure oscillations in the respiratory frequency (systolic, 6.8+/-1.8 to 2.9 +/-0.6 mm Hg2/Hz, P=.017). In contrast, cardiac pacing in the 40 degree tilt position increased arterial pressure variability (systolic, 8.0+/-1.8 to 10.8 +/-2.6, P=.027). Cross-spectral analysis showed that 40 degree tilt shifted the phase relation between systolic pressure and RR interval at the respiratory frequency from positive to negative (9 +/-7 degrees versus -17+/-11 degrees, P=.04); that is, in the supine position, RR interval changes appeared to lead arterial pressure changes, and in the upright position, RR interval changes appeared to follow arterial pressure changes. CONCLUSIONS: These results demonstrate that respiratory sinus arrhythmia can actually contribute to respiratory arterial pressure fluctuations. Therefore, respiratory sinus arrhythmia does not represent simple baroreflex buffering of arterial pressure.

NASA Discipline Cardiopulmonary↗

NASA NTRS1991

Catalysis of hydrolysis and nucleophilic substitution at the P-N bond of phosphoimidazolide-activated nucleotides in phosphate buffers

Phosphoimidazolide-activated derivatives of guanosine and cytidine 5'-monophosphates, henceforth called ImpN's, exhibit enhanced rates of degradation in the presence of aqueous inorganic phosphate in the range 4.0 < or = pH < or = 8.6. This degradation is been attributed to (i) nucleophilic substitution of the imidazolide and (ii) catalysis of the P-N bond hydrolysis by phosphate. The first reaction results in the formation of nucleoside 5'-diphosphate and the second in nucleoside 5'-monophosphate. Analysis of the observed rates as well as the product ratios as a function of pH and phosphate concentration allow distinction between various mechanistic possibilities. The results show that both H2PO4- and HPO4(2-) participate in both hydrolysis and nucleophilic substitution. Statistically corrected biomolecular rate constants indicate that the dianion is 4 times more effective as a general base than the monoanion, and 8 times more effective as nucleophile. The low Bronsted value beta = 0.15 calculated for these phosphate species, presumed to act as general bases in facilitating water attack, is consistent with the fact that catalysis of the hydrolysis of the P-N bond in ImpN's has not been detected before. The beta nuc = 0.35 calculated for water, H2PO4-, HPO4(2-), and hydroxide acting as nucleophiles indicates a more associative transition state for nucleotidyl (O2POR- with R = nucleoside) transfers than that observed for phosphoryl (PO3(2-)) transfers (beta nuc = 0.25). With respect to the stability/reactivity of ImpN's under prebiotic conditions, our study shows that these materials would not suffer additional degradation due to inorganic phosphate, assuming the concentrations of phosphate, Pi, on prebiotic Earth were similar to those in the present oceans ([Pi] approximately 2.25 micromoles).

NASA Discipline Number 52-20↗

NASA NTRS1984

MIDAS - A microcomputer-based image display and analysis system with full Landsat frame processing capabilities

Image Display and Analysis Systems (MIDAS) developed at NASA/Ames for the analysis of Landsat MSS images is described. The MIDAS computer power and memory, graphics, resource-sharing, expansion and upgrade, environment and maintenance, and software/user-interface requirements are outlined; the implementation hardware (including 32-bit microprocessor, 512K error-correcting RAM, 70 or 140-Mbyte formatted disk drive, 512 x 512 x 24 color frame buffer, and local-area-network transceiver) and applications software (ELAS, CIE, and P-EDITOR) are characterized; and implementation problems, performance data, and costs are examined. Planned improvements in MIDAS hardware and design goals and areas of exploration for MIDAS software are discussed.

Hofman, L. B.↗

NASA NTRS1989

Parity retransmission hybrid ARQ using rate 1/2 convolutional codes on a nonstationary channel

A parity retransmission hybrid automatic repeat request (ARQ) scheme is proposed which uses rate 1/2 convolutional codes and Viterbi decoding. A protocol is described which is capable of achieving higher throughputs than previously proposed parity retransmission schemes. The performance analysis is based on a two-state Markov model of a nonstationary channel. This model constitutes a first approximation to a nonstationary channel. The two-state channel model is used to analyze the throughput and undetected error probability of the protocol presented when the receiver has both an infinite and a finite buffer size. It is shown that the throughput improves as the channel becomes more bursty.

Lugand, Laurent R.↗