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At least 127 records · Page 7

Mineralogical studies of sulfide samples and volatile concentrations of basalt glasses from the southern Juan de Fuca Ridge

Sulfide samples obtained from Alvin dives on the southern Juan de Fuca Ridge were examined, showing the presence of two previously undiscovered minerals, both formed at low temperatures. The first detection of lizardite, starkeyite, and anatase in such an environment is also reported. Sulfide geothermometry involving the Cu-Fe-S system shows a vent temperature of less than 328 C for one sample. Ice-melting temperatures on inclusions from this sample are about -2.8 C, and fluid inclusion studies on crystals near this sample show pressure-corrected homogenization temperatures of 268 and 285 C. Volatile concentrations from vesicle-free basalt glass from the vent field are found to be about 0.0013 wt pct CO2 and 0.16 wt pct H2O.

Brett, Robin↗

Exceptional preservation of fossils in an Upper Proterozoic shale

An exceptionally well-preserved and distinctive assemblage of Late Proterozoic fossils from subtidal marine shales is reported. In addition to the spheromorphic acritarchs and cyanobacteria sheaths routinely preserved in Proterozoic rocks, this assemblage includes multicellular algae, a diverse assortment of morphologically complex protistan vesicles, and probable heterotrophic bacteria. Thus, it provides one of the clearest and most taxonomically varied views of Proterozoic life yet reported.

Butterfield, N. J.↗

The Kara and Ust-Kara impact structures (USSR) and their relevance to the K/T boundary event

The Kara and Ust-Kara craters are twin impact structures situated at about 69 deg 10 min N; 65 deg 00 min E at the Kara Sea. For Kara a diameter of about 55 km would be a very conservative estimate, and field observations indicate a maximum current diameter of about 60 km. The diameter of Ust-Kara has to be larger than 16 km. A better estimate might be 25 km but in all likelihood it is even larger. Suevites and impactites from the Kara area have been known since the beginning of the century, but had been misidentified as glacial deposits. Only about 15 years ago the impact origin of the two structures was demonstrated, following the recognition of shock metamorphism in the area. The composition of the target rocks is mirrored by the composition of the clasts within the suevites. In the southern part of Kara, Permian shales and limestones are sometimes accompanied by diabasic dykes, similar to in the central uplift. Due to the high degree of shock metamorphism the shocked magmatic rocks are not easily identified, although most of them seem to be of diabasic or dioritic composition. The impact melts (tagamites) are grey to dark grey fine grained crystallized rocks showing very fine mineral components and are the product of shock-melting with later recrystallization. The impact glasses show a layered structure, inclusions, and vesicles, and have colors ranging from translucent white over brown and grey to black. A complete geochemical characterization of the Kara and Ust-Kara impact craters was attempted by analyzing more than 40 samples of target rocks, shocked rocks, suevites, impact melts, and impact glasses for major and trace elements.

Koeberl, Christian↗

Experiment K-6-03. Gravity and skeletal growth, part 1. Part 2: Morphology and histochemistry of bone cells and vasculature of the tibia; Part 3: Nuclear volume analysis of osteoblast histogenesis in periodontal ligament cells; Part 4: Intervertebral disc swelling pressure associated with microgravity

Bone area, bone electrophysiology, bone vascularity, osteoblast morphology, and osteoblast histogenesis were studied in rats associated with Cosmos 1887. The results suggest that the synchronous animals were the only group with a significantly larger bone area than the basal group, that the bone electrical potential was more negative in flight than in the synchronous rats, that the endosteal osteoblasts from flight rats had greater numbers of transitional Golgi vesicles but no difference in the large Golgi saccules or the alkaline phosphatase activity, that the perioteal vasculature in the shaft of flight rats often showed very dense intraluminal deposits with adjacent degenerating osteocytes as well as lipid accumulations within the lumen of the vessels and sometimes degeneration of the vascular wall (this change was not present in the metaphyseal region of flight animals), and that the progenitor cells decreased in flight rats while the preosteoblasts increased compared to controls. Many of the results suggest that the animals were beginning to recover from the effects of spaceflight during the two day interval between landing and euthanasia; flight effects, such as the vascular changes, did not appear to recover.

Holton, E.↗

A geochemical assessment of possible lunar ore formation

The Moon apparently formed without appreciable water or other relatively volatile materials. Interior concentrations of water or other volatile substances appear to be extremely low. On Earth, water is important to the genesis of nearly all types of ores. Thus, some have reasoned that only abundant elements would occur in ore concentrations. The definition and recognition of ores on the Moon challenge the imaginations and the terrestrial perceptions of ore bodies. Lunar ores included solar-wind soaked soils, which contain abundant but dilute H, C, N, and noble gases (including He-3). Oxygen must be mined; soils contain approximately 45 percent (wt). Mainstream processes of rock formation concentrated Si, Mg, Al, Fe, and Ca, and possibly Ti and Cr. The highland surface contains approximately 70 percent (wt) feldspar (mainly CaAl2Si2O8), which can be separated from some highland soils. Small fragments of dunite were collected; dunite may occur in walls and central peaks of some craters. Theoretical extensions of observations of lunar samples suggest that the Moon may have produced ores of trace elements. Some small fragments have trace-element concentrations 10(exp 4) times higher than the lunar average, indicating that effective geochemical separations occurred; processes included fractional crystallization, silicate immiscibility, vaporization and condensation, and sulfide metamorphism. Operations of these processes acting on indigenous materials and on meteoritic material in the regolith could have produced ores. Infalling carbonaceous meteorites and comets have added water and hydrocarbons that may have been cold-trapped. Vesicles in basalts, pyroclastic beads, and reported transient events suggest gag emission from the lunar interior; such gas might concentrate and transport rare elements. Large impacts may disperse ores or produce them through deposition of heat at depth and by vaporization and subsequent condensation. The main problem in assessing lunar resources is the ignorance about the largely unexplored Moon.

Haskin, Larry A.↗

A window in time for the first evolutionary radiation

The window in time between the last globally sterilizing event and the evidence for a complex procaryotic ecosystem is quite narrow, perhaps as small as 200 million years. We will present a heuristic model outlining the first evolutionary radiation that could have led from primordial vesicles to the universal ancestor. The concept of the universal ancestor will be developed in terms of contemporary molecular biology.

Morowitz, H. J.↗

The cooling rates of pahoehoe flows: The importance of lava porosity

Many theoretical models have been put forward to account for the cooling history of a lava flow; however, only limited detailed field data exist to validate these models. To accurately model the cooling of lava flows, data are required, not only on the heat loss mechanisms, but also on the surface skin development and the causes of differing cooling rates. This paper argues that the cause of such variations in the cooling rates are attributed, primarily, to the vesicle content and degassing history of the lava.

Jones, Alun C.↗

Weathering features and secondary minerals in Antarctic Shergottites ALHA77005 and LEW88516

Previous work has shown that all three sub-groups of the shergottite, nakhlite, and chassignite (SNC) clan of meteorites contain aqueous precipitates of probable pre-terrestrial origin. In the context of secondary minerals, the most thoroughly studied shergottite has been Elephant Moraine, Antarctica A79001 (EETA79001). The recognition of LEW88516 as the latest SNC specimen, and its close similarity with ALHA77005, invite a comparative study of the latter two meteorites, and with EETA79001, from the perspective of aqueous alteration. The fusion crusts of the two meteorites are quite similar except that ALHA77005 is more vesicular (possibly indicating a higher indigenous volatile content). Secondary aluminosilicates (and salts on LEW88516) of definite Antarctic origin partially fill vesicles and fractures on both fusion crusts. Interior samples of the two meteorites are grossly similar in that traces of secondary minerals are present in both.

Wentworth, Susan J.↗

Mapping the distribution of vesicular textures on silicic lavas using the Thermal Infrared Multispectral Scanner

To investigate the effect of vesicularity on TIMS (Thermal Infrared Multispectral Scanner) imagery independent of chemical variations, we studied a large rhyolitic flow of uniform composition but textural heterogeneity. The imagery was recalibrated so that the digital number values for a lake in the scene matched a calculated ideal spectrum for water. TIMS spectra for the lava show useful differences in coarsely and finely vesicular pumice data, particularly in TIMS bands 3 and 4. Images generated by ratioing these bands accurately map out those areas known from field studies to be coarsely vesicular pumice. These texture-related emissivity variations are probably due to the larger vesicles being relatively deeper and separated by smaller septa leaving less smooth glass available to give the characteristic emission of the lava. In studies of inaccessible lava flows (as on Mars) areas of coarsely vesicular pumice must be identified and avoided before chemical variations can be interpreted. Remotely determined distributions of vesicular and glassy textures can also be related to the volatile contents and potential hazards associated with the emplacement of silicic lava flows on Earth.

Ondrusek, Jaime↗

Formation of otoconia in the Japanese red-bellied newt, Cynops pyrrhogaster

Pre-mated adult female newts and fertilized eggs will be flown on the International Microgravity Laboratory-2 flight, in 1994. One objective of the flight will be to observe the influence of microgravity on the development of the gravity-sensing organs in the inner ear. These organs contain sensory hair cells covered by a layer of dense stones (otoconia). Gravity and linear acceleration exert forces on these masses, leading to excitation of the nerve fibers innervating the hair cells. If the production of the otoliths is regulated to reach an optimal weight, their development might be abnormal in microgravity. Ground-based control experiments are reported describing the developmental sequence in which both the otoliths and their associated sensory epithelium and the semicircular canals appear and develop. Three-dimensional reconstruction of serial sections through the otic vesicle of newt embryos at stages 31 through 58 demonstrate the first appearance, relative position and growth of the otoliths. Reports of experiments in which fertilized frog eggs were flown on a Russian Cosmos mission conclude that the utricular otolith is increased in volume, whereas the saccular otolith maintains normal size, suggesting that at least in the utricle, the weight of the otolith might be regulated.

Wiederhold, M. L.↗

Development of the gravity-sensing organs in the Japanese red-bellied newt, Cynops pyrrhogaster

Pre-mated adult female newts and fertilized eggs will be flown on the International Microgravity Laboratory-2 flight, schedule for 1994. One objective of the flight will be to observe the influence of microgravity on the development of the gravity-sensing organs in the inner ear. These organs contain sensory hair cells covered by a layer of dense stones (otoliths). Gravity and linear acceleration exert forces on these masses, leading to excitation of the nerve fibers innervating the hair cells. If the production of the otoliths is regulated to reach an optimal weight, their development would be abnormal in microgravity. Ground-based control experiments are reported describing the developmental sequence in which the otoliths and their associated sensory epithelium appear and increase in size. Three-dimensional reconstruction of serial sections through the otic vesicle of newt embryos at stages 31 through 40 demonstrate the first appearance, relative position and growth of the otoliths. In adult newts, the otoconia in the utricle appear similar to mammalian otoconia, which are composed of calcite. The newt saccular otoconia are at least 99% aragonite, as is found in most aquatic species. Reports of experiments in which fertilized frog eggs were flown on a Russian Cosmos mission conclude that the utricular otolith is increased in volume, whereas the saccular otolith maintains normal size, suggesting that at least the utricular weight might be regulated.

Wiederhold, Michael L.↗

Molecular dynamics studies of simple membrane-water interfaces: Structure and functions in the beginnings of cellular life

Molecular dynamics computer simulations of the structure and functions of a simple membrane are performed in order to examine whether membranes provide an environment capable of promoting protobiological evolution. Our model membrane is composed of glycerol 1-monooleate. It is found that the bilayer surface fluctuates in time and space, occasionally creating thinning defects in the membrane. These defects are essential for passive transport of simple ions across membranes because they reduce the Born barrier to this process by approximately 40%. Negative ions are transferred across the bilayer more readily than positive ions due to favorable interactions with the electric field at the membrane-water interface. Passive transport of neutral molecules is, in general, more complex than predicted by the solubility-diffusion model. In particular, molecules which exhibit sufficient hydrophilicity and lipophilicity concentrate near membrane surfaces and experience 'interfacial resistance' to transport. The membrane-water interface forms an environment suitable for heterogeneous catalysis. Several possible mechanisms leading to an increase of reaction rates at the interface are discussed. We conclude that vesicles have many properties that make them very good candidates for earliest protocells. Some potentially fruitful directions of experimental and theoretical research on this subject are proposed.

Pohorille, Andrew↗

Effusive silicic volcanism in the Central Andes: The Chao dacite and other young lavas of the Altiplano-Puna Volcanic Complex

The largest known Quaternary silicic lava body in the world is Cerro Chao in north Chile, a 14-km-long coulee with a volume of at least 26 cu km. It is the largest of a group of several closely similar dacitic lavas erupted during a recent (less than 100,000 year old) magmatic episode in the Altiplano-Puna Volcanic Complex (APVC; 21-24 deg S) of the Centra; Andean Volcanic Zone. The eruption of Chao proceeded in three phases. Phase 1 was explosive and produced approximately 1 cu km of coarse, nonwelded dacitic pumice deposits and later block and ash flows that form an apron in front of the main lava body. Phase 2 was dominantly effusive and erupted approximately 22.5 cu km of magma in the form of a composite coulee covering approximately 53 sq km with a 400-m-high flow front and a small cone of poorly expanded pumice around the vent. The lava is homogeneous with rare flow banding and vesicular tops and selvages. Ogives (flow ridges) reaching heights of 30 m form prominent features on its surface. Phase 3 produced a 6-km-long, 3-km-wide flow that emanated from a collapsed dome. Ogives are subdued, and the lava is glassier than that produced in previous phases. All the Chao products are crystal-rich high-K dacites and rhyodacites with phenocrysts of plagioclase, quartz, hornblende, biotite, sphene, rare snidine, and oxides. Phenocryst contents reach 40-60 vol % (vesicle free) in the main phase 2 lavas but are lower in the phase 1 (20-25%) and phase 3 (approximately 40%) lavas. Ovoid andesitic inclusions with vesicular interiors and chilled margins up to 10 cm are found in the later stages of phase 2 and compose up to 5% of the phase 3 lava. There is little evidence for preeruptive zonation of the magma body in composition, temperature (approximately 840 C), fO2 (19(exp -11), or water content, so we propose that eruption of the Chao complex was driven by intrusion of fresh, hot andesitic magma into a crystallizing and largely homogeneous body of dacitic magma. Morphological measurements suggest that the Chao lavas had internal plastic viscosities of 10(exp 10) to 10(exp 12) Pa s, apparent viscosities of 10(exp 9) Pa s, surface viscosities of 10(exp 15) to 10(exp 24) Pa s, and a yield strength of 8 x 10(exp 5) Pa. These estimates indicate that Chao would have exhibited largely similar rheological properties to other silicic lava extrusions, notwithstanding its high phenocryst content. We suggest that Chao's anomalous size is a function of both the relatively steep local slope (20 deg to 3 deg) and the available volume of magma. The eruption duration for Chao's emplacement is thought to have been about 100 to 150 years, with maximum effusion rates of about 25 cu m/s for short periods. Four other lavas in the vicinity with volumes of approximately 5 cu km closely resemble Chao and are probably comagnetic. The suite as a whole shares a petrologic and chemical similarity with the voluminous regional Tertiary to Pleistocene ignimbrites of the APVC and may be derived from a zone of silicic magmatism that is thought to have been active since the late Tertiary. Chao and the other young lavas may represent either the waning of this system or a new episode fueled by intrusions of mafic magma.

De Silva, S. L.↗

Experiment K-7-18: Effects of Spaceflight in the Muscle Adductor Longus of Rats Flown in the Soviet Biosatellite Cosmos 2044: A Study Employing Neural Cell Adhesion Molecules (N-CAM) Immunocytochemistry and Conventional Morphological Techniques (Light and Electron Microscopy) - Part 1

The effects of spaceflight upon the 'slow' muscle adductor longus was examined in rats flown in the Soviet Biosatellite COSMOS 2044. Three groups - synchronous, vivarium and basal served as controls. The techniques employed included standard methods for light microscopy, N-CAM immunocytochemistry and electron microscopy. Light microscopic observations revealed myofiber atrophy, contraction bands and segmental necrosis accompanied by cellular infiltrates composed of macrophages, leucocytes and mononuclear cells. N-CAM immunoreactivity was seen (N-CAM-IR) on the myofiber surface, satellite cells and in regenerating myofibers reminiscent of myotubes. Ultrastructural alterations included Z band streaming, disorganization of myofibrillar architecture, sarcoplasmic degradation, extensive segmental necrosis with preservation of the basement membrane, degenerative phenomena of the capillary endothelium and cellular invasion of necrotic areas. Regenerating myofibers were identified by the presence of increased amounts of ribosomal aggregates and chains of polyribosomes associated with myofilaments that displayed varied distributive patterns. The principal electron microscopic changes of the neuromuscular junctions consisted of a decrease or absence of synaptic vesicles, degeneration of axon terminals, increased number of microtubules, vacant axonal spaces and axonal sprouting. The present observations indicate that major alterations such as myofibrillar disruption and necrosis, muscle regeneration and denervation and synaptic remodeling at the level of the neuromuscular junction may take place during spaceflight.

Daunton, N. G.↗

[Ribosomal RNA Evolution]

It is generally believed that an RNA World existed at an early stage in the history of life. During this early period, RNA molecules are seen to be potentially involved in both catalysis and the storage of genetic information. Translation presents several interrelated themes of inquiry for exobiology. First, it is essential, for understanding the very origin of life, how peptides and eventually proteins might have come to be made on the early Earth in a template directed manner. Second, it is necessary to understand how a machinery of similar complexity to that found in the ribosomes of modern organisms came to exist by the time of the last common ancestor (as detected by 16S rRNA sequence studies). Third, the ribosomal RNAs themselves likely had a very early origin and studies of their history may be very informative about the nature of the RNA World. Moreover, studies of these RNAs will contribute to a better understanding of the potential roles of RNA in early evolution.During the past year we have ave conducted a comparative study of four completely sequenced bacterial genoames. We have focused initially on conservation of gene order. The second component of the project continues to build on the model system for studying the validity of variant 5S rRNA sequences in the vicinity of the modern Vibrio proteolyticus 5S rRNA that we established earlier. This system has made it possible to conduct a detailed and extensive analysis of a local portion of the sequence space. These core methods have been used to construct numerous mutants during the last several years. Although it has been a secondary focus, this work has continued over the last year such that we now have in excess of 125 V. proteolyticus derived constructs which have been made and characterized. We have also continued high resolution NMR work on RNA oligomers originally initiated by G. Kenneth Smith who was funded by a NASA Graduate Student Researcher's Fellowship Award until May of 1996. Mr. Smith developed synthesis & purification protocols in order to obtain large quantities of RNA oligomers for NMR studies. He succeeded in obtaining preliminary one dimensional spectra for an analog of the helix II/loop B/helix III domain of 5S rRNAs well as a 29mer which includes helix III and the highly characteristic 13 nucleotide loop C. Significant progress in laying the required groundwork for this work has been accomplished during the past year. A plasmid, pLeuS-1, carrying the E. coli leucine tRNA synthetase gene (LeuRS) has been obtained and used to overexpressed the leucine synthetase. A T7 runoff transcription has been established in our laboratory and successfully used to make both minihelix RNAs and tRNAs. In addition, encapsulation studies have been conducted and we have successfully encapsulated tRNA in lipid vesicles. Initial efforts to obtain peptide bond synthesis will be underway over the next several moths.

Source record↗

Membrane-Based Functions in the Origin of Cellular Life

Our objective is to help explain how the earliest ancestors of contemporary cells (protocells) performed their essential functions employing only the molecules available in the protobiological milieu. Our hypothesis is that vesicles, built of amphiphilic, membrane-forming materials, emerged early in protobiological evolution and served as precursors to protocells. We further assume that the cellular functions associated with contemporary membranes, such as capturing and, transducing of energy, signaling, or sequestering organic molecules and ions, evolved in these membrane environments. An alternative hypothesis is that these functions evolved in different environments and were incorporated into membrane-bound structures at some later stage of evolution. We focus on the application of the fundamental principles of physics and chemistry to determine how they apply to the formation of a primitive, functional cell. Rather than attempting to develop specific models for cellular functions and to identify the origin of the molecules which perform these functions, our goal is to define the structural and energetic conditions that any successful model must fulfill, therefore providing physico-chemical boundaries for these models. We do this by carrying out large-scale, molecular level computer simulations on systems of interest.

Chipot, Christophe↗

Fluid Inclusions in Extraterrestrial Samples Fact or Fiction?

Over the years there have been numerous reports of liquid inclusions in meteorites. Roedder reviews the reported occurrences of liquid inclusions in meteorites and states that "silicate-melt inclusions are expectable and apparently ubiquitous, but the presence of actual liquid inclusions (i.e., with moving bubbles at room temperature) would seem almost impossible." The reason for this conclusion is that meteorites (presumably) form in space at high temperatures and very low pressures where liquid water (or carbon dioxide) is not stable. Perhaps the most infamous report of fluid inclusions in meteorites was that of Warner et al. In that study, the authors reported the presence of two-phase, liquid-vapor inclusions in a diogenite from Antarctica. This report of fluid inclusions generated considerable interest in the meteorite community, and caused many to question existing models for the origin of the diogenites. This interest was short-lived however, as later investigations of the same samples showed that the inclusions were most likely artifacts. Rudnick et al. showed that many of the inclusions in meteorites prepared at the Johnson Space Center contained a fluid that fluoresced strongly under the laser beam on the Raman microprobe. They interpreted this to indicate that the inclusions contained Almag oil used in the preparation of thin sections. Presumably, the Almag oil entered empty vesicles along fractures that were opened intermittently during cutting. Here, the occurrence of unambiguous fluid inclusions that could not have been introduced during sample preparation are described in samples from two different extraterrestrial environments. One environment is represented by the SNC (martian) meteorites ALH 84001 and Nakhla. The second environment is represented by the Monahans 1998 meteorite that fell recently in the USA.

Bodnar, R. J.↗

Possible Bacteria in Nakhla

Mars Agueous Alteration Nakhla contains alteration products in an extensive network of which fill cracks, veins, and void spaces, composed of smectite, although in some cases they are amorphous and gel-like. Strong evidence presented show that these alteration products developed on Mars: some veins are offset by impact faulting of olivine followed by annealing of the olivine, and veins near the fusion crust show partial vesicle development suggesting heating and volatile liberation. The smectite-containing cracks and veins are interpreted as low temperature aqueous alteration on Mars. We have been studying these veins with optical microscopy and high resolution SEM.

McKay, David S., Jr.↗