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At least 127 records · Page 7

Proceedings of Minnowbrook Workshops I to VI

This DVD collection includes the complete proceedings of Minnowbrook Workshops I through VI. Titles include Minnowbrook I - 1993 Workshop on End-Stage Boundary Layer Transition (NASA/CP-2007-214667, CASI ID 20070038942), Minnowbrook II - 1997 Workshop on Boundary Layer Transition in Turbomachines (NASA/CP-1998-206958, CASI ID 19980206205), Minnowbrook III - 2000 Workshop on Boundary Layer Transition and Unsteady Aspects of Turbomachinery Flow (NASA/CP-2001-210888, CASI ID 20020067662), Minnowbrook IV - 2003 Workshop on Transition and Unsteady Aspects of Turbomachinery Flows (NASA TM-2004-212913, CASI ID 20040121174), Minnowbrook V - 2006 Workshop on Unsteady Flows in Turbomachinery (NASA/CP-2006-214484, CASI ID 20070024781), and Minnowbrook VI - 2009 Workshop on Flow Physics and Control for Internal and External Aerodynamics (NASA/CP-2010-216112, CASI ID 20100018557).

John E. Lagraff↗

Injury Risk Assessment of Extravehicular Mobility Unit (EMU) Phase VI and Series 4000 Gloves During Extravehicular Activity (EVA) Hand Manipulation Tasks

Functional Extravehicular Mobility Units (EMUs) with high precision gloves are essential for the success of Extravehicular Activity (EVA). Previous research done at NASA has shown that total strength capabilities and performance are reduced when wearing a pressurized EMU. The goal of this project was to characterize the human-space suit glove interaction and assess the risk of injury during common EVA hand manipulation tasks, including pushing, pinching and gripping objects. A custom third generation sensor garment was designed to incorporate a combination of sensors, including force sensitive resistors, strain gauge sensors, and shear force sensors. The combination of sensors was used to measure the forces acting on the finger nails, finger pads, finger tips, as well as the knuckle joints. In addition to measuring the forces, data was collected on the temperature, humidity, skin conductance, and blood perfusion of the hands. Testing compared both the Phase VI and Series 4000 glove against an ungloved condition. The ungloved test was performed wearing the sensor garment only. The project outcomes identified critical landmarks that experienced higher workloads and are more likely to suffer injuries. These critical landmarks varied as a function of space suit glove and task performed. The results showed that less forces were acting on the hands while wearing the Phase VI glove as compared to wearing the Series 4000 glove. Based on our findings, the engineering division can utilize these methods for optimizing the current space suit glove and designing next generation gloves to prevent injuries and optimize hand mobility and comfort.

Kilby, Melissa↗

Latest NASA Instrument Cost Model (NICM): Version VI

The NASA Instrument Cost Model, NICM, is a suite of tools which allow for probabilistic cost estimation of NASA's space-flight instruments at both the system and subsystem level. NICM also includes the ability to perform cost by analogy as well as joint confidence level (JCL) analysis. The latest version of NICM, Version VI, was released in Spring 2014. This paper will focus on the new features released with NICM VI, which include: 1) The NICM-E cost estimating relationship, which is applicable for instruments flying on Explorer-like class missions; 2) The new cluster analysis ability which, alongside the results of the parametric cost estimation for the user's instrument, also provides a visualization of the user's instrument's similarity to previously flown instruments; and 3) includes new cost estimating relationships for in-situ instruments.

Mrozinski, Joe↗

A conserved chaperone protein is required for the formation of a noncanonical type VI secretion system spike tip complex

Type VI secretion systems (T6SSs) are dynamic protein nanomachines found in Gram-negative bacteria that deliver toxic effector proteins into target cells in a contact-dependent manner. Prior to secretion, many T6SS effector proteins require chaperones and/or accessory proteins for proper loading onto the structural components of the T6SS apparatus. However, despite their established importance, the precise molecular function of several T6SS accessory protein families remains unclear. In this study, we set out to characterize the DUF2169 family of T6SS accessory proteins. Using gene co-occurrence analyses, we find that DUF2169-encoding genes strictly co-occur with genes encoding T6SS spike complexes formed by valine-glycine repeat protein G (VgrG) and DUF4150 domains. Although structurally similar to Pro-Ala-Ala-Arg (PAAR) domains, “PAAR-like” DUF4150 domains lack PAAR motifs and instead contain a conserved PIPY motif, leading us to designate them PIPY domains. Next, we present both genetic and biochemical evidence that PIPY domains require a cognate DUF2169 protein to form a functional T6SS spike complex with VgrG. This contrasts with canonical PAAR proteins, which bind VgrG on their own to form functional spike complexes. By solving the first crystal structure of a DUF2169 protein, we show that this T6SS accessory protein adopts a novel protein fold. Furthermore, biophysical and structural modeling data suggest that DUF2169 contains a dynamic loop that physically interacts with a hydrophobic patch on the surface of its cognate PIPY domain. Based on these findings, we propose a model whereby DUF2169 proteins function as molecular chaperones that maintain VgrG–PIPY spike complexes in a secretion-competent state prior to their export by the T6SS apparatus.

DUF2169↗

Materials Data on VI by Materials Project

VI is Wurtzite structured and crystallizes in the hexagonal P6_3mc space group. The structure is three-dimensional. V is bonded to four equivalent I atoms to form distorted corner-sharing VI4 trigonal pyramids. There are one shorter (2.79 Å) and three longer (2.81 Å) V–I bond lengths. I is bonded to four equivalent V atoms to form distorted corner-sharing IV4 trigonal pyramids.

36 MATERIALS SCIENCE↗