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At least 127 records · Page 7
All sourcesNASA NTRSDOE OSTI
NASA NTRS1990

NAE Twin Otter operations in FIFE 1989

During the summer of 1989, the National Aeronautical Establishment (Canada) Twin Otter Atmospheric Research Aircraft was flow in support of the NASA sponsored First ISLSCP (International Satellite Land Surface Climatology Project) Field Experiment called FIFE-89. Airborne measurements of the fluxes of heat, momentum, water vapor and carbon dioxide were made during 16 flow-altitude flights over the FIFE project area in central Kansas. The Twin Otter operations in FIFE are documented and details are included on the instrumentation, software, flight procedures, atmospheric conditions and analysis methods. Comparisons of Twin Otter measured fluxes are made with those determined at several surface sites and with those made by other instrumented aircraft in the FIFE-87 measurements. Airborne flux measurements are related to run length, altitude, and environmental parameters such as vegetation type, temperature, and wind speed. One night flight was run in an attempt to measure the respiration component of the CO2 flux. The use of aircraft was studied for regional observations of fluxes and to relate these to satellite radiance measurements. Run average data are presented for all 285 flux runs flown by the Twin Otter in FIFE-89. This should serve as as a working reference for scientists utilizing Twin Otter data either directly of through the FIFE data archive.

Macpherson, J. I.↗
DOE OSTI2021

Warm-season net CO 2 uptake outweighs cold-season emissions over Alaskan North Slope tundra under current and RCP8.5 climate

Abstract Arctic warming has increased vegetation growth and soil respiration during recent decades. The rate of Arctic warming will likely amplify over the 21st century. Previous studies have revealed that the most severe Arctic warming occurred during the cold season (September to May). The cold-season warming has posited significant CO 2 emissions to the atmosphere via respiration, possibly offsetting warm-season (June to August) net CO 2 uptake. However, prevailing Earth system land models poorly represent cold-season CO 2 emissions, making estimates of Arctic tundra annual CO 2 budgets highly uncertain. Here, we demonstrate that an improved version of the energy exascale Earth system model (E3SM) land model (ELMv1-ECA) captures the large amount of cold-season CO 2 emissions over Alaskan Arctic tundra as reported by two independent, observationally-constrained datasets. We found that the recent seven-decades warming trend of cold-season soil temperature is three times that of the warm-season. The climate sensitivity of warm-season net CO 2 uptake, however, is threefold higher than for the cold-season net CO 2 loss, mainly due to stronger plant resilience than microbial resilience to hydroclimatic extremes. Consequently, the modeled warm-season net CO 2 uptake has a larger positive trend (0.74 ± 0.14 gC m −2 yr −1 ) than that of cold-season CO 2 emissions (0.64 ± 0.11 gC m −2 yr −1 ) from 1950 to 2017, supported by enhanced plant nutrient uptake and increased light- and water-use efficiency. With continued warming and elevated CO 2 concentrations under the representative concentration pathway (RCP) 8.5 scenario, the increasing rate of warm-season net CO 2 uptake is more than twice the rate of cold-season emissions (1.33 ± 0.32 gC m −2 yr −1 vs 0.50 ± 0.12 gC m −2 yr −1 ), making the modeled Alaskan Arctic tundra ecosystem a net CO 2 sink by 2100. However, other geomorphological and ecological disturbances (e.g. abrupt permafrost thaw, thermokarst development, landscape-scale hydrological changes, wildfire, and insects) that are not considered here might alter our conclusion.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Characterization of Materials Used as Face Coverings for Respiratory Protection

Use of masks is a primary tool to prevent the spread of the novel COVID-19 virus resulting from unintentional close contact with infected individuals. However, detailed characterization of the chemical properties and physical structure of common mask materials is lacking in the current literature. In this study, a series of commercial masks and potential mask materials, including 3M Particulate Respirator 8210 N95, a material provided by Oak Ridge National Laboratory Carbon Fiber Technology Facility (ORNL/CFTF), and a Filti Face Mask Material, were characterized by a suite of techniques, including scanning electron microscopy, X-ray diffraction, Raman spectroscopy, and X-ray photoelectron spectroscopy. Wetting properties of the mask materials were quantified by measurements of contact angle with a saliva substitute. Mask pass-through experiments were performed using a dispersed metal oxide nanoparticle suspension to model the SARS-CoV-2 virus, with quantification via spatially resolved X-ray fluorescence mapping. Notably, all mask materials tested provided a strong barrier against respiratory droplet breakthrough. The comparisons and characterizations provided in this study provide useful information when evaluating mask materials for respiratory protection.

36 MATERIALS SCIENCE↗
NASA NTRS2020

Empirical estimates of regional carbon budgets imply reduced global soil heterotrophic respiration

Resolving regional carbon budgets is critical for informing land-based mitigation policy. For nine regions covering nearly the whole globe, we collected inventory estimates of carbon-stock changes complemented by satellite estimates of biomass changes where inventory data are missing. The net land–atmospheric carbon exchange (NEE) was calculated by taking the sum of the carbon-stock change and lateral carbon fluxes from crop and wood trade, and riverine-carbon export to the ocean. Summing up NEE from all regions, we obtained a global ‘bottom-up’ NEE for net land anthropogenic CO2 uptake of –2.2±0.6 PgC/yr consistent with the independent top-down NEE from the global atmospheric carbon budget during 2000–2009. This estimate is so far the most comprehensive global bottom-up carbon budget accounting, which set up an important milestone for global carbon-cycle studies. By decomposing NEE into component fluxes, we found that global soil heterotrophic respiration amounts to a source of CO2 of 39 PgC/yr with an interquartile of 33–46 PgC/yr —a much smaller portion of net primary productivity than previously reported.

carbon budget↗
DOE OSTI2020

Empirical estimates of regional carbon budgets imply reduced global soil heterotrophic respiration

Resolving regional carbon budgets is critical for informing land-based mitigation policy. For nine regions covering nearly the whole globe, we collected inventory estimates of carbon-stock changes complemented by satellite estimates of biomass changes where inventory data are missing. The net land–atmospheric carbon exchange (NEE) was calculated by taking the sum of the carbon-stock change and lateral carbon fluxes from crop and wood trade, and riverine-carbon export to the ocean. Summing up NEE from all regions, we obtained a global ‘bottom-up’ NEE for net land anthropogenic CO2 uptake of –2.2 ± 0.6 PgC yr -1 consistent with the independent top-down NEE from the global atmospheric carbon budget during 2000–2009. This estimate is so far the most comprehensive global bottom-up carbon budget accounting, which set up an important milestone for global carbon-cycle studies. By decomposing NEE into component fluxes, we found that global soil heterotrophic respiration amounts to a source of CO 2 of 39 PgC yr -1 with an interquartile of 33–46 PgC yr -1 —a much smaller portion of net primary productivity than previously reported.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS2022

Soil Respiration Phenology Improves Modeled Phase of Terrestrial Net Ecosystem Exchange in Northern Hemisphere

In the northern hemisphere, terrestrial ecosystems transition from net sources of CO2 to the atmosphere in winter to net ecosystem carbon sinks during spring. The timing (or phase) of this transition, determined by the balance between ecosystem respiration (RECO) and primary production, is key to estimating the amplitude of the terrestrial carbon sink. We diagnose an apparent phase bias in the RECO and net ecosystem exchange (NEE) seasonal cycles estimated by the Terrestrial Carbon Flux (TCF) model framework and investigate its link to soil respiration mechanisms. Satellite observations of vegetation canopy conditions, surface meteorology, and soil moisture from the NASA SMAP Level 4 Soil Moisture product are used to model a daily carbon budget for a global network of eddy covariance flux towers. Proposed modifications to TCF include: the inhibition of foliar respiration in the light (the Kok effect); a seasonally varying litterfall phenology; an O2 diffusion limitation on heterotrophic respiration (RH); and a vertically resolved soil decomposition model. We find that RECO phase bias can result from bias in RECO magnitude and that mechanisms which reduce northern spring RECO, like substrate and O2 diffusion limitations, can mitigate the phase bias. A vertically resolved soil decomposition model mitigates this bias by temporally segmenting and lagging RH. Applying these model enhancements at Continuous Soil Respiration (COSORE) sites verifies their improvement of RECO and NEE skill compared to in situ observations (up to ∆RMSE = −0.76 g C m−2 d −1 35 ). Ultimately, these mechanisms can improve prior estimates of NEE for atmospheric inversion studies.

Soil respiration↗
DOE OSTI2021

Springtime Drought Shifts Carbon Partitioning of Recent Photosynthates in 10-Year Old Picea mariana Trees, Causing Restricted Canopy Development

Springtime bud-break and shoot development induces substantial carbon (C) costs in trees. Drought stress during shoot development can impede C uptake and translocation. This is therefore a channel through which water shortage can lead to restricted shoot expansion and physiological capacity, which in turn may impact annual canopy C uptake. We studied effects of drought and re-hydration on early season shoot development, C uptake and partitioning in five individual 10-year old Picea mariana [black spruce] trees to identify and quantify dynamics of key morphological/physiological processes. Trees were subjected to one of two treatments: (i) well-watered control or (ii) drought and rehydration. We monitored changes in morphological [shoot volume, leaf mass area (LMA)], biochemical [osmolality, non-structural carbohydrates (NSC)] and physiological [rates of respiration (R d ) and light-saturated photosynthesis ( A sat )] processes during shoot development. Further, to study functional compartmentalization and use of new assimilates, we 13 C-pulse labeled shoots at multiple development stages, and measured isotopic signatures of leaf respiration, NSC pools and structural biomass. Shoot water potential dropped to a minimum of −2.5 MPa in shoots on the droughted trees. Development of the photosynthetic apparatus was delayed, as shoots on well-watered trees broke-even 14 days prior to shoots from trees exposed to water deficit. R d decreased with shoot maturation as growth respiration declined, and was lower in shoots exposed to drought. We found that shoot development was delayed by drought, and while rehydration resulted in recovery of A sat to similar levels as shoots on the well-watered trees, shoot volume remained lower. Water deficit during shoot expansion resulted in longer, yet more compact (i.e., with greater LMA) shoots with greater needle osmolality. The 12 C: 13 C isotopic patterns indicated that internal C partitioning and use was dependent on foliar developmental and hydration status. Shoots on drought-stressed trees prioritized allocating newly fixed C to respiration over structural components. In conclusion, temporary water deficit delayed new shoot development and resulted in greater LMA in black spruce. Since evergreen species such as black spruce retain active foliage for multiple years, impacts of early season drought on net primary productivity could be carried forward into subsequent years.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS2002

Performance of advanced trauma life support procedures in microgravity

BACKGROUND: Medical operations on the International Space Station will emphasize the stabilization and transport of critically injured personnel and so will need to be capable of advanced trauma life support (ATLS). METHODS: We evaluated the ATLS invasive procedures in the microgravity environment of parabolic flight using a porcine animal model. Included in the procedures evaluated were artificial ventilation, intravenous infusion, laceration closure, tracheostomy, Foley catheter drainage, chest tube insertion, peritoneal lavage, and the use of telemedicine methods for procedural direction. RESULTS: Artificial ventilation was performed and appeared to be unaltered from the 1-G environment. Intravenous infusion, laceration closure, percutaneous dilational tracheostomy, and Foley catheter drainage were achieved without difficulty. Chest tube insertion and drainage were performed with no more difficulty than in the 1-G environment due to the ability to restrain patient, operator and supplies. A Heimlich valve and Sorenson drainage system were both used to provide for chest tube drainage collection with minimal equipment, without the risk of atmospheric contamination, and with the capability to auto-transfuse blood drained from a hemothorax. The use of telemedicine in chest tube insertion was demonstrated to be useful and feasible. Peritoneal lavage using a percutaneous technique, although requiring less training to perform, was found to be dangerous in weightlessness due to the additional pressure of the bowel on the anterior abdominal wall creating a high risk of bowel perforation. CONCLUSIONS: The performance of ATLS procedures in microgravity appears to be feasible with the exception of diagnostic peritoneal lavage. Minor modifications to equipment and techniques are required in microgravity to effect surgical drainage in the presence of altered fluid dynamics, to prevent atmospheric contamination, and to provide for the restraint requirements. A parabolic simulation system was developed for equipment and procedure verification, physiological research, and possible crew medical officer training in the future.

Parabolic Flight↗
DOE OSTI2023

The Effect of Rapid Development on Soil CO2 Efflux in a Cellulosic Biofuel Stand

As awareness of climate change increases, the need for carbon neutral fuel sources is growing. Lignocellulosic biofuel derived from pine trees has been suggested as one potential energy source; however, it requires more research before its efficacy for climate change mitigation can be determined. Due to the large share of forest carbon held in soils and the extensive area of pine plantations in the southeast U.S., a better understanding of plantation soil carbon dynamics is critical for biofuel carbon accounting. This study evaluated the effects of canopy development and productivity on soil CO2 efflux, a proxy for soil respiration (Rs), in an intensively managed loblolly pine (Pinus taeda) stand over a period from May 2015 to December 2019. We found that leaf area index (LAI) and gross ecosystem production (GEP), as well as meteorological variables, had significant effects on Rs, but that both overall Rs and soil carbon pools did not increase over the course of the study. We thus hypothesize that GEP and LAI had intra-annual effects on Rs, and that the lack of change in Rs is the result of an increase in autotrophic respiration (Ra) that offset a decrease in decomposition of the previous stand’s organic matter.

09 BIOMASS FUELS↗
DOE OSTI2020

Improving Arsenic Tolerance of Pyrococcus furiosus by Heterologous Expression of a Respiratory Arsenate Reductase

Arsenate is a notorious toxicant that is known to disrupt multiple biochemical pathways. Many microorganisms have developed mechanisms to detoxify arsenate using the ArsC-type arsenate reductase, and some even use arsenate as a terminal electron acceptor for respiration involving arsenate respiratory reductase (Arr). ArsC-type reductases have been studied extensively, but the phylogenetically unrelated Arr system is less investigated and has not been characterized from Archaea. Here, we heterologously expressed the genes encoding Arr from the crenarchaeon Pyrobaculum aerophilum in the euryarchaeon Pyrococcus furiosus, both of which grow optimally near 100°C. Recombinant P. furiosus was grown on molybdenum (Mo)- or tungsten (W)-containing medium, and two types of recombinant Arr enzymes were purified, one containing Mo (Arr-Mo) and one containing W (Arr-W). Purified Arr-Mo had a 140-fold higher specific activity in arsenate [As(V)] reduction than Arr-W, and Arr-Mo also reduced arsenite [As(III)]. The P. furiosus strain expressing Arr-Mo (the Arr strain) was able to use arsenate as a terminal electron acceptor during growth on peptides. In addition, the Arr strain had increased tolerance compared to that of the parent strain to arsenate and also, surprisingly, to arsenite. Compared to the parent, the Arr strain accumulated intracellularly almost an order of magnitude more arsenic when cells were grown in the presence of arsenite. Furthermore, x-ray absorption spectroscopy (XAS) results suggest that the Arr strain of P. furiosus improves its tolerance to arsenite by increasing production of less-toxic arsenate and nontoxic methylated arsenicals compared to that by the parent.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Cysteine Rich Intestinal Protein 2 is a copper-responsive regulator of skeletal muscle differentiation and metal homeostasis

Copper (Cu) is essential for respiration, neurotransmitter synthesis, oxidative stress response, and transcription regulation, with imbalances leading to neurological, cognitive, and muscular disorders. Here we show the role of a novel Cu-binding protein (Cu-BP) in mammalian transcriptional regulation, specifically on skeletal muscle differentiation using murine primary myoblasts. Utilizing synchrotron X-ray fluorescence-mass spectrometry, we identified murine cysteine-rich intestinal protein 2 (mCrip2) as a key Cu-BP abundant in both nuclear and cytosolic fractions. mCrip2 binds two to four Cu + ions with high affinity and presents limited redox potential. CRISPR/Cas9-mediated deletion of mCrip2 impaired myogenesis, likely due to Cu accumulation in cells. CUT&RUN and transcriptome analyses revealed its association with gene promoters, including MyoD1 and metallothioneins, suggesting a novel Cu-responsive regulatory role for mCrip2. Our work describes the significance of mCrip2 in skeletal muscle differentiation and metal homeostasis, expanding understanding of the Cu-network in myoblasts. Copper (Cu) is essential for various cellular processes, including respiration and stress response, but imbalances can cause serious health issues. This study reveals a new Cu-binding protein (Cu-BP) involved in muscle development in primary myoblasts. Using unbiased metalloproteomic techniques and high throughput sequencing, we identified mCrip2 as a key Cu-BP found in cell nuclei and cytoplasm. mCrip2 binds up to four Cu + ions and has a limited redox potential. Deleting mCrip2 using CRISPR/Cas9 disrupted muscle formation due to Cu accumulation. Further analyses showed that mCrip2 regulates the expression of genes like MyoD1, essential for muscle differentiation, and metallothioneins in response to copper supplementation. This research highlights the importance of mCrip2 in muscle development and metal homeostasis, providing new insights into the Cu-network in cells.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1974

Volatilisation of terpenes from Salvia mellifera

The study demonstrates significant terpene volatilisation from Salvia mellifera. Net photosynthesis and dark respiration were measured in an intact branch of a potted plant using a gas analysis system. Photosynthesis and respiration rates were determined for various temperatures. The rates were directly proportional to leaf temperature and were the same in both light and dark reactions. Using the temperature curve for the steady-state rate of terpene volatilisation and the gas exchange characteristics, the daily carbon gain and terpene loss were calculated.

Tyson, B. J.↗
NASA NTRS2000

A Quantitative Study of Oxygen as a Metabolic Regulator

An acute reduction in oxygen delivery to a tissue is associated with metabolic changes aimed at maintaining ATP homeostasis. However, given the complexity of the human bio-energetic system, it is difficult to determine quantitatively how cellular metabolic processes interact to maintain ATP homeostasis during stress (e.g., hypoxia, ischemia, and exercise). In particular, we are interested in determining mechanisms relating cellular oxygen concentration to observed metabolic responses at the cellular, tissue, organ, and whole body levels and in quantifying how changes in tissue oxygen availability affect the pathways of ATP synthesis and the metabolites that control these pathways. In this study; we extend a previously developed mathematical model of human bioenergetics, to provide a physicochemical framework that permits quantitative understanding of oxygen as a metabolic regulator. Specifically, the enhancement - sensitivity analysis - permits studying the effects of variations in tissue oxygenation and parameters controlling cellular respiration on glycolysis, lactate production, and pyruvate oxidation. The analysis can distinguish between parameters that must be determined accurately and those that require less precision, based on their effects on model predictions. This capability may prove to be important in optimizing experimental design, thus reducing use of animals.

Radhakrishnan, Krishnan↗
NASA NTRS1994

Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes

Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox indicator which can be reduced by respiring bacteria to fluorescent CTC-formazan crystals. Rh 123 is incorporated into bacteria with respect to cellular proton motive force. The intracellular accumulation of these fluorescent dyes can be determined using epifluorescence microscopy. The results obtained with these two fluorescent probes in situ were compared to the plate count (PC) and in situ direct viable count (DVC) methods. Viable cell densities within biofilms determined by the three in situ methods were comparable and always showed approximately 2-fold higher values than those obtained with the PC method. As an additional advantage, the results were observed after 2 h, which was shorter than the 4 h incubation time required for the DVC method and 24 h for colony formation. The results indicate that staining with CTC and Rh 123 provides rapid information regarding cell numbers and physiological activities of bacteria within biofilms.

NASA Discipline Number 04-10↗
NASA NTRS2003

A quantitative study of oxygen as a metabolic regulator

An acute reduction in oxygen delivery to a tissue is associated with metabolic changes aimed at maintaining ATP homeostasis. However, given the complexity of the human bioenergetic system, it is difficult to determine quantitatively how cellular metabolic processes interact to maintain ATP homeostasis during stress (e.g., hypoxia, ischemia, and exercise). In particular, we are interested in determining mechanisms relating cellular oxygen concentration to observed metabolic responses at the cellular, tissue, organ, and whole body levels and in quantifying how changes in tissue oxygen availability affect the pathways of ATP synthesis and the metabolites that control these pathways. In this study, we extend a previously developed mathematical model of human bioenergetics, to provide a physicochemical framework that permits quantitative understanding of oxygen as a metabolic regulator. Specifically, the enhancement--sensitivity analysis--permits studying the effects of variations in tissue oxygenation and parameters controlling cellular respiration on glycolysis, lactate production, and pyruvate oxidation. The analysis can distinguish between parameters that must be determined accurately and those that require less precision, based on their effects on model predictions. This capability may prove to be important in optimizing experimental design, thus reducing use of animals.

Non-programmatic↗
NASA NTRS1997

System identification of closed-loop cardiovascular control: effects of posture and autonomic blockade

We applied system identification to the analysis of fluctuations in heart rate (HR), arterial blood pressure (ABP), and instantaneous lung volume (ILV) to characterize quantitatively the physiological mechanisms responsible for the couplings between these variables. We characterized two autonomically mediated coupling mechanisms [the heart rate baroreflex (HR baroreflex) and respiratory sinus arrhythmia (ILV-HR)] and two mechanically mediated coupling mechanisms [the blood pressure wavelet generated with each cardiac contraction (circulatory mechanics) and the direct mechanical effects of respiration on blood pressure (ILV-->ABP)]. We evaluated the method in humans studied in the supine and standing postures under control conditions and under conditions of beta-sympathetic and parasympathetic pharmacological blockades. Combined beta-sympathetic and parasympathetic blockade abolished the autonomically mediated couplings while preserving the mechanically mediated coupling. Selective autonomic blockade and postural changes also altered the couplings in a manner consistent with known physiological mechanisms. System identification is an "inverse-modeling" technique that provides a means for creating a closed-loop model of cardiovascular regulation for an individual subject without altering the underlying physiological control mechanisms.

NASA Discipline Number 18-10↗
DOE OSTI2020

Performance of Closed Loop Venturi Aspirated Aeration System: Experimental Study and Numerical Analysis with Discrete Bubble Model

In wastewater treatment plants, aeration plays a significant role as it encourages aerobic respiration of microbes, which are necessary to break down carbonaceous matter in the waste stream. This process can account for the majority of energy use in wastewater treatment plants. The aeration process is also necessary in odor control in lagoons and in the aquaculture industry. Generally, the aeration process is accomplished with compressors or blowers which can be of low efficiency due to ideal gas laws. This study introduces the idea of increasing aeration efficiency by looping water from a reservoir through a piping network which includes a venturi aspirator at its inlet. For this purpose, an experimental study has been conducted in a laboratory setup with a pump which pulls water from a tank, passes it through a Venturi aspirator and a helical piping network intended to increase bubble residence time, before depositing it back into the bulk fluid tank. This same process is modeled computationally, using a discrete bubble method (DBM), with good agreement with experiments. The overall purpose here is to determine the optimal configurations for standard aeration efficiency (SAE) and the standard oxygen transfer rate (SOTR). A parametric study has been implemented using the DBM based on different hydraulic and flow parameters. The model is also used to predict the SAE of a hypothetical aeration system. Results indicate that it is possible to achieve SAE values in the range of surface aerators or submersed jet aerators using the proposed aeration system with less complex components, thereby decreasing overall costs.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗
NASA NTRS1994

FIFE data analysis: Testing BIOME-BGC predictions for grasslands

The First International Satellite Land Surface Climatology Project (ISLSCP) Field Experiment (FIFE) was conducted in a 15 km by 15 km research area located 8 km south of Manhattan, Kansas. The site consists primarily of native tallgrass prairie mixed with gallery oak forests and croplands. The objectives of FIFE are to better understand the role of biology in controlling the interactions between the land and the atmosphere, and to determine the value of remotely sensed data for estimating climatological parameters. The goals of FIFE are twofold: the upscale integration of models, and algorithm development for satellite remote sensing. The specific objectives of the field campaigns carried out in 1987 and 1989 were the simultaneous acquisition of satellite, atmospheric, and surface data; and the understanding of the processes controlling surface energy and mass exchange. Collected data were used to study the dynamics of various ecosystem processes (photosynthesis, evaporation and transpiration, autotrophic and heterotrophic respiration, etc.). Modelling terrestrial ecosystems at scales larger than that of a homogeneous plot led to the development of simple, generalized models of biogeochemical cycles that can be accurately applied to different biomes through the use of remotely sensed data. A model was developed called BIOME-BGC (for BioGeochemical Cycles) from a coniferous forest ecosystem model, FOREST-BGC, where a biome is considered a combination of a life forms in a specified climate. A predominately C4-photosynthetic grassland is probably the most different from a coniferous forest possible, hence the FIFE site was an excellent study area for testing BIOME-BGC. The transition from an essentially one-dimensional calculation to three-dimensional, landscape scale simulations requires the introduction of such factors as meteorology, climatology, and geomorphology. By using remotely sensed geographic information data for important model inputs, process-based ecosystem simulations at a variety of scales are possible. The second objective of this study is concerned with determining the accuracy of the estimated fluxes from BIOME-BGC, when extrapolated spatially over the entire 15-km by 15-km FIFE site. To accomplish this objective, a topographically distributed map of soil depth at the FIFE site was developed. These spatially-distributed fluxes were then tested with data from aircraft by eddy-flux correlation obtained during the FIFE experiment.

Hunt, E. Raymond, Jr.↗