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At least 127 records · Page 7
All sourcesNASA NTRSDOE OSTI
NASA NTRS1978

Estimates on the production of CO and H2 from the oxidation of hydrocarbon emissions from vegetation

Extrapolating from extensive field measurements on foliar emissions in the U.S. approximate global inputs of isoprene and terpenes of 3.5 times 10 to the 14th power and 4.8 times 10 to the 14th power g(C)/yr, respectively, are obtained. The oxidation of these hydrocarbons could contribute in an important way to the atmospheric sources of CO (4.2-13.3 times 10 to the 14th power g/yr) and H2 (10-35 times 10 to the 12th power g/yr), and to organic species soluble in rainwater

Zimmerman, P. R.↗
NASA NTRS1978

Configurations of base-pair complexes in solutions

A theoretical search for the most stable conformations (i.e., stacked or hydrogen bonded) of the base pairs A-U and G-C in water, CCl4, and CHCl3 solutions is presented. The calculations of free energies indicate a significant role of the solvent in determining the conformations of the base-pair complexes. The application of the continuum method yields preferred conformations in good agreement with experiment. Results of the calculations with this method emphasize the importance of both the electrostatic interactions between the two bases in a complex, and the dipolar interaction of the complex with the entire medium. In calculations with the solvation shell method, the last term, i.e., dipolar interaction of the complex with the entire medium, was added. With this modification the prediction of the solvation shell model agrees both with the continuum model and with experiment, i.e., in water the stacked conformation of the bases is preferred.

Egan, J. T.↗
NASA NTRS1984

Preliminary design of two Space Shuttle fluid physics experiments

The mid-deck lockers of the STS and the requirements for operating an experiment in this region are described. The design of the surface tension induced convection and the free surface phenomenon experiments use a two locker volume with an experiment unique structure as a housing. A manual mode is developed for the Surface Tension Induced Convection experiment. The fluid is maintained in an accumulator pre-flight. To begin the experiment, a pressurized gas drives the fluid into the experiment container. The fluid is an inert silicone oil and the container material is selected to be comparable. A wound wire heater, located axisymmetrically above the fluid can deliver three wattages to a spot on the fluid surface. These wattages vary from 1-15 watts. Fluid flow is observed through the motion of particles in the fluid. A 5 mw He/Ne laser illuminates the container. Scattered light is recorded by a 35mm camera. The free surface phenomena experiment consists of a trapezoidal cell which is filled from the bottom. The fluid is photographed at high speed using a 35mm camera which incorporated the entire cell length in the field of view. The assembly can incorporate four cells in one flight. For each experiment, an electronics block diagram is provided. A control panel concept is given for the surface induced convection. Both experiments are within the mid-deck locker weight and c-g limits.

Gat, N.↗
NASA NTRS1986

Investigations with methanobacteria and with evolution of the genetic code

Mycoplasma capricolum was found by Osawa et al. to use UGA as the code of tryptophan and to contain 75% A + T in its DNA. This change could have been from evolutionary pressure to replace C + G by A + T. Numerous studies have been reported of evolution of proteins as measured by amino acid replacements that are observed when homologus proteins, such as hemoglobins from various vertebrates, are compared. These replacements result from nucleotide substitutions in amino acid codons in the corresponding genes. Simultaneously, silent nucleotide substitutions take place that can be studied when sequences of the genes are compared. These silent evolutionary changes take place mostly in third positions of codons. Two types of nucleotide substitutions are recognized: pyrimidine-pyrimidine and purine-purine interchanges (transitions) and pyriidine-purine interchanges (transversions). Silent transitions are favored when a corresponding transversion would produce an amino acid replacement. Conversely, silent transversions are favored by probability when transitions and transversions will both be silent. Extensive examples of these situations have been found in protein genes, and it is evident that transversions in silent positions predominate in family boxes in most of the examples studied. In associated research a streptomycete from cow manure was found to produce an extracellular enzyme capable of lysing the pseudomurein-contining methanogen Methanobacterium formicicum.

Jukes, T. H.↗
NASA NTRS1986

High frequency sampling of the 1984 spring bloom within the mid-Atlantic Bight: Synoptic shipboard, aircraft, and in situ perspectives of the SEEP-I experiment

Moorings of current meters, thermistors, transmissometers, and fluorometers on the mid-Atlantic shelf, south of Long Island, suggest a cumulative seaward export of perhaps 0.35 g C/sq m/day between the 80 and 120 m isobaths during February-April 1984. Such a horizontal loss of algal carbon over the lower third of the water column would be 23 to 78% of the March-April 1984 primary production. This physical carbon loss is similar to daily grazing losses from zooplankton of 32-40% of the algal fixation of carbon. Metabolic demands of the benthos could be met by just the estimated fecal pellet flux, without direct consumption of algal carbon, while bacterioplankton needs could be served by excretory release of dissolved organic matter during photosynthesis. Sediment traps tethered 10 m off the bottom at the 120 m isobath and 50 m above the 500 m isobath caught as much as 0.16 to 0.26 g C /sq m/day during March-April 1984, in reasonable agreement with the flux estimated from the other moored instruments.

Walsh, J. J.↗
NASA NTRS1985

A change in the genetic code in Mycoplasma capricolum

Mycoplasma capricolum was previously found to use UGA instead of UGG as its codon for tryptophan and to contain 75 percent A + T in its DNA. The codon change could have been due to mutational pressure to replace C + G by A + T, resulting in the replacement of UGA stop codons by UAA, change of the anticodon in tryptophan tRNA from CCA to UCA, and replacement of UGG tryptophan codons by UGA. None of these changes should have been deleterious.

Jukes, T. H.↗
NASA NTRS1988

Theoretical study of the first and second negative systems of N2(+)

Extended Gaussian basis sets and large CASSCF/multireference CI wave functions are used in theoretical determination of the potential curves for the X 2Sigma(+)g, B 2Sigma(+)g, C 2Sigma(+)u, (1) 2Delta(u), (2) 2Delta(u), 4Pi(u), and 2Sigma(-)u states of N2(+). The results, including the radial dependence of the electronic transition moments, the Einstein coefficients, the radiative lifetimes, and the crossing points between selected states, are presented in extensive tables and graphs and discussed in detail.

Langhoff, Stephen R.↗
NASA NTRS1921

Statical longitudinal stability of airplanes

This report, which is a continuation of the "Preliminary report on free flight testing" (report no. NACA-TR-70), presents a detailed theoretical analysis of statical stability with free and locked controls and also the results of many free flight test on several types of airplanes. In developing the theory of stability with locked controls an expression for pitching moment is derived in simple terms by considering the total moment as the sum of the moments due to wings and tail surface. This expression, when differentiated with respect to angle of incidence, enables an analysis to be made of the factors contributing to the pitching moment. The effects of slipstream and down wash are also considered and it is concluded that the C. G. Location has but slight effect or stability, and that stability is much improved by increasing the efficiency of the tail surfaces, which may be done by using an "inverted" tail plane. The results of free flight tests with locked controls are discussed at length and it is shown that the agreement between the experimental results and theory is very satisfactory. The theory of stability with free controls is not amendable to the simple mathematical treatment used in the case of locked controls, but a clear statement of the conditions enables several conclusions to be drawn, one of which is that the fixed tail surfaces should be much larger than the movable surfaces.

Warner, Edward P↗
NASA NTRS1930

Tables for pressure of air on coming to rest from various speeds

In Technical Report no. 247 of the National Advisory Committee for Aeronautics theoretical formulas are given from which was computed a table for the pressure of air on coming to rest from various speeds, such as those of aircraft and propeller blades. In that report, the table gave incompressible and adiabatic stop pressures of air for even-speed intervals in miles per hour and for some even-speed intervals in knots per hour. Table II of the present report extends the above-mentioned table by including the stop pressures of air for even-speed intervals in miles per hour, feet per-second, knots per hour, kilometers per hour, and meters per second. The pressure values in table II are also more exact than values given in the previous table. To furnish the aeronautical engineer with ready numerical formulas for finding the pressure of air on coming to rest, table I has been derived for the standard values specified below it. This table first presents the theoretical pressure-speed formulas and their working forms in C. G. S. Units as given in NACA Technical Report No. 247, then furnishes additional working formulas for several special units of speed. (author)

Zahm, A F↗
NASA NTRS1992

Carbon cycling in high-latitude ecosystems

The carbon-rich soils and peatlands of high-latitude ecosystems could substantially influence atmospheric concentrations of CO2 and CH4 in a changing climate. Currently, cold, often waterlogged conditions retard decomposition, and release of carbon back to the atmosphere may be further slowed by physical protection of organic matter in permafrost. As a result, many northern ecosystems accumulate carbon over time (Billings et al., 1982; Poole and Miller, 1982), and although such rates of accumulation are low, thousands of years of development have left Arctic ecosystems with an extremely high soil carbon content; Schlesinger's (1984) average value of 20.4 kg C/m(sup 2) leads to a global estimate of 163 x 10(exp 15) g C. All GCM simulations of a doubled CO2 climate predict the greatest warming to occur in the polar regions (Dickinson, 1986; Mitchell, 1989). Given the extensive northern carbon pools and the strong sensitivity of decomposition processes to temperature, even a slight warming of the soil could dramatically alter the carbon balance of Arctic ecosystems. If warming accelerates rates of decomposition more than rates of primary production, a sizeable additional accumulation of CO2 in the atmosphere could occur. Furthermore, CH4 produced in anaerobic soils and peatlands of the Arctic already composes a good percentage of the global efflux (Cicerone and Oremlund, 1988); if northern soils become warmer and wetter as a whole, CH4 emissions could dramatically rise. A robust understanding of the primary controls of carbon fluxes in Arctic ecosystems is critical. As a framework for a systematic examination of these controls, we discussed a conceptual model of regional-scale Arctic carbon turnover, including CH4 production, and based upon the Century soil organic matter model.

Townsend, Alan↗
NASA NTRS1994

Evaluation of bio-optical algorithms to remotely sense marine primary production from space

In situ bio-optical measurements from several oceanographic campaigns were analyzed to derive a direct relationship between water column primary production P (sub t) ocean color as expressed by the ratio of reflectances R (sub 1) at 440 nm and R (sub 3) at 550 nm and photosynthetically available radiation (PAR). The study is restricted to the Morel case I waters for which the following algorithm is proposed: log (P(sub f)) = -4.286 - 1.390 log (R(sub 1)/R(sub3)) + 0.621 log (PAR), with P(sub t) in g C m(exp -2)/d and PAR in J m(exp -2)/d. Using this algorithm the rms accuracy of primary production estimate is 0.17 on a logarithmic scale, i.e., a factor of 1.5. Using spectral reflectance measurements in the entire visible spectral range, the central wavelength, spectral bandwidth, and radiometric noise level requirements are investigated for the channels to be used by an ocean color space mission dedicated to estimating global marine primary production and the associated carbon fluxes. Nearly all the useful information is provided by two channels centered at 440 nm and 550 nm, but the accuracy of primary production estimate appears weakly sensitive to spectral bandwidth, which, consequently, may be enlarged by several tens of nanometers. The sensitivity to radiometric noise, on the contrary, is strong, and a noise equivalent reflectance of 0.005 degraded the accuracy on the primary production estimate by a factor 2 (0.14-0.25 on a logarithmic scale). The results should be applicable to evaluating the primary production of oligotrophic and mesotrophic waters, which constitute most of the open ocean.

Berthelot, Beatrice↗
NASA NTRS1995

Winds and accretion in delta Sagittae

The ten-year binary delta Sge (M2 Ib-II+B9.5 V) is a zeta Aur binary containing an abnormally cool component. Combining our analysis of the system as a visual binary with Batten's radial-velocity solution leads to the following properties: i = 40 deg, a = 51 mas = 8.83 A.U. = 1893 solar radius, hence d = 173 pc; M(sub B) = 2.9 solar mass and M(sub M) = 3.8 solar mass; and R(sub B) = 2.6 solar radius and R(sub M) = 152 solar radius. This interpretation of the orbit places the M supergiant on the asymptotic giant branch. We have collected ultraviolet spectra throughout the star's 1980-90 orbit, concentrated around the conjuction of 1990. The wind of the M giant appears in these as narrow shell lines of singly ionized metals, chiefly Fe II, with P-Cyg profiles at many phases, which show the slow variation in strength expected for the orbit but no pronounced atmospheric eclipse. The terminal velocity of the wind is 16-18 km/s, and its excitation temperature is approximately 10,000 K. Most of the broadening of the wind lines is caused by differential expansion of the atmosphere, with (unmeasurably) low turbulent velocities. Nontheless, the mass loss rate (1.1 +/- 0.4 X 10 (exp -8) solar mas/yr) is almost the same as found previously by Reimers and Schroder for very different assumptions about the velocity structure. Also seen in the spectrum throughout the orbit are the effects of a variable, high-speed wind as well as evidence for accretion onto the B9.5 star. This high-speed wind absorbs in species of all ionization stages observed, e. g., C II, Mg II, Al III, SI IV, C IV, and has a terminaal velocity in the range 200-450 km/s. We presume this wind originates at the B dwarf, not the M supergiant, and speculate that it comes from an accretion disk, as suggested by recent models of magnetically moderated accretion. Evidence for accretion is redshifted absorption in the same transitions formed in the high-speed wind, as well as broad emission lines of singly ionized metals. This emission seems to be scattered out of the continuum of the B star. Finally, we discuss Auger ionization by accretion luminosity as the cause of the high ionization in these high-speed flows as well as the source of the extended circumstellar clouds of Si(3+) and C(3+) ions in zeta Aur binaries.

Eaton, Joel A.↗
NASA NTRS1998

Detecting a Terrestrial Biosphere Sink for Carbon Dioxide: Interannual Ecosystem Modeling for the Mid-1980s

There is considerable uncertainty as to whether interannual variability in climate and terrestrial ecosystem production is sufficient to explain observed variation in atmospheric carbon content over the past 20-30 years. In this paper, we investigated the response of net CO2 exchange in terrestrial ecosystems to interannual climate variability (1983 to 1988) using global satellite observations as drivers for the NASA-CASA (Carnegie-Ames-Stanford Approach) simulation model. This computer model of net ecosystem production (NEP) is calibrated for interannual simulations driven by monthly satellite vegetation index data (NDVI) from the NOAA Advanced Very High Resolution Radiometer (AVHRR) at 1 degree spatial resolution. Major results from NASA-CASA simulations suggest that from 1985 to 1988, the northern middle-latitude zone (between 30 and 60 degrees N) was the principal region driving progressive annual increases in global net primary production (NPP; i.e., the terrestrial biosphere sink for carbon). The average annual increase in NPP over this predominantly northern forest zone was on the order of +0.4 Pg (10 (exp 15) g) C per year. This increase resulted mainly from notable expansion of the growing season for plant carbon fixation toward the zonal latitude extremes, a pattern uniquely demonstrated in our regional visualization results. A net biosphere source flux of CO2 in 1983-1984, coinciding with an El Nino event, was followed by a major recovery of global NEP in 1985 which lasted through 1987 as a net carbon sink of between 0.4 and 2.6 Avg C per year. Analysis of model controls on NPP and soil heterotrophic CO2 fluxes (Rh) suggests that regional warming in northern forests can enhance ecosystem production significantly. In seasonally dry tropical zones, periodic drought and temperature drying effects may carry over with at least a two-year lag time to adversely impact ecosystem production. These yearly patterns in our model-predicted NEP are consistent in magnitude with the estimated exchange of CO2 by the terrestrial biosphere with the atmosphere, as determined by previous isotopic (delta (sup 13 C) convolution analysis. Ecosystem simulation results can help further target locations where net carbon sink fluxes have occurred in the past or may be verified in subsequent field studies.

Potter, Christopher S.↗
NASA NTRS2002

Autocommander: A Supervisory Controller for Integrated Guidance and Control for the 2nd Generation Reusable Launch Vehicle

This paper presents a hierarchical architecture for integrated guidance and control that achieves risk and cost reduction for NASA's 2d generation reusable launch vehicle (RLV). Guidance, attitude control, and control allocation subsystems that heretofore operated independently will now work cooperatively under the coordination of a top-level autocommander. In addition to delivering improved performance from a flight mechanics perspective, the autocommander is intended to provide an autonomous supervisory control capability for traditional mission management under nominal conditions, G&C reconfiguration in response to effector saturation, and abort mode decision-making upon vehicle malfunction. This high-level functionality is to be implemented through the development of a relational database that is populated with the broad range of vehicle and mission specific data and translated into a discrete event system model for analysis, simulation, and onboard implementation. A Stateflow Autocoder software tool that translates the database into the Stateflow component of a Matlab/Simulink simulation is also presented.

Fisher, J. E.↗
NASA NTRS2002

Assembly of Colloidal Materials Using Bioadhesive Interactions

We have pursued the use of biological crosslinking molecules of several types to make colloidal materials at relatively low volume fraction of colloidal particles. The objective is to make binary alloys of colloidal particles, made of two different colloidal particles coated with complementary biological lock-and-key binding molecules, which assemble due to the biological specificity. The long-term goal is to use low affinity lock-and-key biological interactions, so that the can anneal to form crystalline states. We have used a variety of different surface chemistries in order to make colloidal materials. Our first system involved using selectin-carbohydrate (sialyl-Lewis) interactions; this chemistry is derived from immune system. This chemical interaction is of relatively low affinity, with timescales for dissociation of several seconds. Furthermore, the adhesion mediated by these molecules can be reversed by the chelation of calcium atoms; thus assembled structures can be disassembled reversibly. Our second system employed avidin-biotin chemistry. This well-studied system is of high affinity, and is generally irreversible on a laboratory time-scale. Thus, we would expect selectin-carbohydrate interactions at high molecular density and avidin-biotin interactions to give kinetically-trapped structures; however, at low densities, we would expect significant differences in the structure and dynamics of the two materials, owing to their very different release rates. We have also begun to use a third chemistry - DNA hybridization. By attaching single stranded DNA oligonucleotide chains to beads, we can drive the assembly of colloidal materials by hybridization of complementary DNA chains. It is well known that DNA adenosine-thymine (A-T) and guanine-cytosine (G-C) bases hybridize pairwise with a Gibbs free energy change of 1.7 kcal/mol per base; thus, the energy of the assembly can be modulated by altering the number of complementary bases in the DNA chains. Using these different crosslinking molecules, we have assembled colloidal materials from different-sized colloidal particles, A and B. In the first sets of experiment, we used high densities of adhesion molecules, and 0.96 micron (A) and 5.5 micron (B) diameter particles. The high density of adhesion molecules means that the structures are kinetically trapped in nonequilibrium configurations. The structure of the suspension can be varied by changing the number ratio of the two types of colloidal particles, NA and NB, where A is the smaller particle. With carbohydrate-selectin or avidin-biotin interactions, large NA/NB leads to the formation of colloidal micelles, with the large center B particle surrounded by many smaller A particles. As the ratio NA/NB decreases, the structures become more extended, approaching the formation of macro-Rouse polymers - extended linear chains where A beads are connected with intervening small B linkers.

Hammer, Daniel A.↗
NASA NTRS2003

Effects of Microsecond Pulse Laser Irradiation on Vis-NIR Reflectance Spectrum of Carbonaceous Chondrite Simulant: Implications for Martian Moons and Primitive Asteroids

Goal of this study is to make a progress in understanding the optical effects of space weathering on small bodies believed to be similar in composition to carbonaceous chondrites: C, G, B, F, T, D, and P asteroids and possibly Martian satellites Phobos and Deimos. The companion work focuses on petrological and mineralogical aspects of this process. One of the main factors of space weathering is meteorite and micrometeorite bombardment leading, in particular, to impact melting of components of the regolith. Studies of lunar regolith and laboratory experiments simulating impact melting show that the melting products differ from the unmelted material in mineralogy and distribution of chemical components among different phases that results in spectral changes. We simulate impact melting of CM chondrite by pulse laser irradiation of an artificial analog of such a meteorite. The analog is a mixture of 46 wt.% non-magnetic fraction of L5 ordinary chondrite Tsarev, 47 wt.% serpentine, 5 wt.% kerite, and 2 wt.% calcite. It simulates rather well bulk chemistry, including volatiles such as H2O and CO2, and only approximately the CM chondrite mineralogy. Thus, we do not expect the mixture to be spectrally similar to CM chondrites, but expect the laser melting products to be similar to those formed by impact melting of natural CM chondrites.

Hiroi, T.↗
NASA NTRS2003

Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant

One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA-DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, gamma-radiation, H2O2 and desiccation. The 18S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92T and Bacillus benzoevorans DSM 5391T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA-DNA hybridization data support the proposal of FO-92T as Bacillus nealsonii sp. nov. (type strain is FO-92T =ATCC BAAM-519T =DSM 15077T).

Non-programmatic↗
NASA NTRS2002

Spatial distribution of total, ammonia-oxidizing, and denitrifying bacteria in biological wastewater treatment reactors for bioregenerative life support

Bioregenerative life support systems may be necessary for long-term space missions due to the high cost of lifting supplies and equipment into orbit. In this study, we investigated two biological wastewater treatment reactors designed to recover potable water for a spacefaring crew being tested at Johnson Space Center. The experiment (Lunar-Mars Life Support Test Project-Phase III) consisted of four crew members confined in a test chamber for 91 days. In order to recycle all water during the experiment, an immobilized cell bioreactor (ICB) was employed for organic carbon removal and a trickling filter bioreactor (TFB) was utilized for ammonia removal, followed by physical-chemical treatment. In this study, the spatial distribution of various microorganisms within each bioreactor was analyzed by using biofilm samples taken from four locations in the ICB and three locations in the TFB. Three target genes were used for characterization of bacteria: the 16S rRNA gene for the total bacterial community, the ammonia monooxygenase (amoA) gene for ammonia-oxidizing bacteria, and the nitrous oxide reductase (nosZ) gene for denitrifying bacteria. A combination of terminal restriction fragment length polymorphism (T-RFLP), sequence, and phylogenetic analyses indicated that the microbial community composition in the ICB and the TFB consisted mainly of Proteobacteria, low-G+C gram-positive bacteria, and a Cytophaga-Flexibacter-Bacteroides group. Fifty-seven novel 16S rRNA genes, 8 novel amoA genes, and 12 new nosZ genes were identified in this study. Temporal shifts in the species composition of total bacteria in both the ICB and the TFB and ammonia-oxidizing and denitrifying bacteria in the TFB were also detected when the biofilms were compared with the inocula after 91 days. This result suggests that specific microbial populations were either brought in by the crew or enriched in the reactors during the course of operation.

NASA Discipline Life Support Systems↗