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At least 127 records · Page 7

Visual, Optical and Replica Inspections: Surface Preparation of 650 MHz NB Cavity for PIP-II Linac

Surface preparation of niobium superconducting RF cavities is a critical step for achieving good RF performance under the superconducting state. Surface defect, roughness, and contamination affect the accelerating gradient and quality factor of the cavities. We report surface inspection methods used to control the surface processing of 650 MHz cavities designated for the pre-production and prototype cryomodules for PIP-II linac. The cavity surface was routinely inspected visually, with an optical camera, and by microscopic scanning of surface replicas. This article covers details on the surface inspection methods and surface polishing process used to repair the surface.

43 PARTICLE ACCELERATORS↗

Laser size reduction of contaminated Magnox Pond skips - 15166

The Magnox Pond Program Team at Hinkley Point A Power Station have successfully demonstrated the use of an automated Computer Numeric Controlled (CNC) dry milling process to efficiently decontaminate radioactive pond skips that were once used to store and transport Magnox spent fuel assemblies between reactor sites and Sellafield for reprocessing. This decontamination process has ensured ILW skips meet metal melt recycling criteria, and even Out of Scope levels, while at the same time producing dry swarf that is easily disposed of as ILW. However, the dry milling method requires the skips to be size reduced to a very simple flat geometry to simplify the milling operation. Several size reduction techniques (mechanical and thermal) have been evaluated. Based on the advantages of remote handling, high cutting speed, flexibility with complex geometries, reduced secondary waste production and significantly reduced worker exposure, robotically operated fibre delivered laser cutting was chosen. This paper describes the development, installation and operation of an automated laser cutting system commissioned at Hinkley Point A to size reduce contaminated skips in to five flat sections. It was demonstrated that a complete skip can be remotely size reduced in less than 60 minutes. This successful demonstration, coupled with CNC milling, has the potential to save an estimated British Pounds 30 million across the Magnox sites alone, with much higher saving potentials for skips stored at Sellafield, enabling this new size reduction process to meet cost reduction objectives of the UK's Nuclear Decommissioning Authority. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Mercury contaminated sediment sites—An evaluation of remedial options

Mercury (Hg) is a naturally-occurring element that is ubiquitous in the aquatic environment. Though efforts have been made in recent years to decrease Hg emissions, historically-emitted Hg can be retained in the sediments of aquatic bodies where they may be slowly converted to methylmercury (MeHg). Consequently, Hg in historically-contaminated sediments can result in high levels of significant exposure for aquatic species, wildlife and human populations consuming fish. Even if source control of contaminated wastewater is achievable, it may take a very long time, perhaps decades, for Hg-contaminated aquatic systems to reach relatively safe Hg levels in both water and surface sediment naturally. It may take even longer if Hg is present at higher concentration levels in deep sediment. Hg contaminated sediment results from previous releases or ongoing contributions from sources that are difficult to identify. Due to human activities or physical, chemical, or biological processes (e.g. hydrodynamic flows, bioturbation, molecular diffusion, and chemical transformation), the buried Hg can be remobilized into the overlying water. Hg speciation in the water column and sediments critically affect the reactivity (i.e. conversion of inorganic Hg(II) to MeHg), transport, and its exposure to living organisms. Also, geochemical conditions affect the activity of methylating bacteria and its availability for methylation. This review paper discusses remedial considerations (e.g. key chemical factors in fate and transport of Hg, source characterization and control, environmental management procedures, remediation options, modeling tools) and includes practical case studies for cleaning up Hg-contaminated sediment sites. -- Highlights: ► Managing mercury-contaminated sediment sites are challenging to remediate. ► Remediation technologies are making a difference in managing these sites. ► Partitioning plays a dominant role in the distribution of mercury species. ► Mathematical models can be used to help us understand the chemistry and processes.

60 APPLIED LIFE SCIENCES↗

Investigation of dominant hydrogeochemical processes influencing uranium transport in groundwater at a retired explosives test site - 15727

The Lawrence Livermore National Laboratory (LLNL) Site 300 facility was established in 1955 to support US weapons research and development. Open-air explosives tests, that used depleted uranium as a proxy, were conducted at the Building 812 site. Depleted uranium was released into the environment, eventually migrating into the underlying sandstone bedrock aquifer. At the site, groundwater concentrations of uranium exceed the California Maximum Contaminant Level of 20 pico-Curies per liter (pCi/L). However, the groundwater contaminant plume appears to attenuate within 60 meters of the contaminant source, beyond which no depleted uranium is observed. The goal of this research is to determine the processes (i.e., sorption to mineral surfaces, biotransformation, diffusion, dispersion, and mineral precipitation) controlling attenuation of uranium in local groundwater. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Aerial Lidar and Geomorphic Change Detection Software- Tools for Monitoring Canyon Sediments at LANL - 18668

The Los Alamos National Laboratory Environmental Remediation program has conducted extensive geomorphic surveys of sediment deposits in the canyon bottoms within and surrounding the facility. The goal of the geomorphic surveys was to provide basic information needed to characterize chemical inventories of the sediments downstream of historical release sites. Sediment units were mapped based on depositional setting and age. Representative characterization samples were collected for each unit and submitted for chemical analysis. Chemical inventories were estimated by calculating the total volume of each depositional unit and multiplying by average chemical concentration. This method of characterization provides a spatial distribution of chemical data based on fluvial processes. In general, for the canyon bottom sediments, the higher concentrations of industrial chemicals were associated with fine-grained floodplain deposits closest to the source. Since initial characterization, many of the reaches have undergone changes in response to post-wildfire floods and severe rain events. These events impacted the channel and floodplain deposits. Much of the elevated concentrations are confined to the over-bank deposits distal from the primary channel. In order to ensure that the sediment units containing elevated chemical concentrations are stable, repeat aerial light detection and ranging (LiDAR) surveys are conducted and changes are assessed using the Geomorphic Change Detection (GCD) plug-in for ArcGIS. These tools provide high confidence uncertainty estimates and extensive areal coverage. This is a valuable method for long-term monitoring of surface sediments with low-level but widespread contaminant distributions. All of the monitored canyon bottom reaches pass risk assessment and present technically infeasible sediment removal options. Following the 2014 monsoon season, aerial LiDAR data has been collected annually and the resulting digital elevation models (DEMs) processed using the GCD method. Changes are identified using GCD and are verified by field walk downs. If erosion is identified in areas which are vulnerable to mobilizing contaminated sediment, enhanced controls can be installed to stabilize banks or other areas with high erosion potential. Annual sediment monitoring samples are collected where there is evidence for flow and deposition within the past year. Since 2014, no severe floods have been catalogued using the LiDAR surveys and GCD software. The recent rainfall/runoff events have not caused major changes to the canyon bottom channels and floodplains. Geomorphic processes identified in the monitoring results include channel aggradation and incision that over the course of a monsoon season results in minor changes to the system. Other active processes contributing to observed changes are minor slides, flows, slumps, and falls of unconsolidated sediment on steep bedrock or soil surfaces. A comprehensive baseline has been established by geomorphic mapping and characterization sampling. Periodic monitoring consists of LiDAR surveys and GCD analysis which offers an effective and defensible methodology for documenting stability of surface sediments. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Natural Gas Drilling near the Underground Nuclear Test Site of Rulison, Colorado - 19571

In 1969, the Project Rulison test was conducted in western Colorado using a nuclear device to stimulate natural gas production by fracturing low-permeability, gas-bearing rock. The presence of radionuclides (specifically tritium) in the produced gas persisted above acceptable levels, and the test was abandoned. Advances in hydrofracturing technology over the past 10-15 years have revolutionized the extraction of hydrocarbons from low-permeability formations, leading to a significant increase of drilling in the Rulison area. Drilling activity nearing the Rulison site has raised concerns that remnant radioactivity might be encountered. The site, now under the care of the US Department of Energy (DOE), is protected by a 16-hectare (40 acre) institutional control boundary (Lot 11) that prohibits drilling or extraction of materials below 1830 meters (6000 feet) within the lot boundary. Drilling within 0.8 kilometer (0.5 mile) of the site requires a hearing before the Colorado Oil and Gas Conservation Commission. DOE recommends that gas developers adopt a conservative, staged drilling approach, allowing gas reserves near the Rulison site to be recovered in a manner that minimizes the likelihood of encountering contamination. The staged approach calls for collecting data from wells away from the site before drilling closer. For instance, the wells 1.2 kilometer (0.75 mile) west of the site did not encounter any unexpected geologic conditions and now have 8 years of production without encountering any detonation-related radionuclides. The next set of wells would be 0.8 kilometer (0.5 mile) west of the site. This sequential method drills first at locations with a low probability of encountering contamination and then moves closer. This process protects public safety while allowing the collection of additional data to confirm that contamination has not migrated beyond the 16-hectare (40-acre) institutional control boundary. The staged drilling approach was simulated with a modeling effort that estimates the current extent of contamination and the potential for production-induced contaminant migration to nearby wells. The model incorporates geologic data from nearby wells and was calibrated to production data from both the reentry well production testing (1970-1971) and the wells 1.2 kilometer (0.75 mile) from the site that began producing in 2010. The geologic data include both sand/shale distribution statistics as well as the tendency of hydrofractures to preferentially propagate east and west due to the regional stress field. The model results indicate that it is unlikely that wells near the site would ever encounter site-related radionuclides. (authors)

03 NATURAL GAS↗

Electrolytically Assisted Surface Decontamination (EASD{sup TM}) for POCO Operations - 20282

NNL in collaboration with C-Tech Innovation Ltd and Sellafield Ltd has been exploring innovative technologies to enable a significant reduction of radiological hazards within facilities during the Post Operational Clean Out (POCO) phase of a nuclear plant's life cycle. Reducing the hazard by effectively decontaminating plants in-situ during POCO delivers huge cost reductions for future decommissioning operations. These cost reduction benefits are realized by reducing the number and complexity of remote operations as well as lowering the long-term waste disposal costs. Whilst chemical decontamination can achieve the desired level of decontamination, applying aggressive chemical reagents is hazardous, potentially difficult to control and requires there to be complimentary effluent treatment and waste routes. This work has aimed to develop flexible and controllable decontamination processes which could be operated without the additional complexity and issues associated with chemical decontamination. The processes needed to be relatively fast and effective to minimize the time operators would spend in an active area. In addition, there was a driver to produce decontamination methodologies which generated a secondary waste compatible with current routes and which takes advantage of current waste capacity. The strategy therefore was to come up with a solution that works with, rather than against, the science and engineering behind process plants at Sellafield. Electrolytically Assisted Surface Decontamination (EASD{sup TM}) is an innovative electrochemical decontamination process (developed by NNL and C-Tech Innovation Ltd) that can remove activity from contaminated metal in very short time periods. Application of the patented electric waveform to the surface when treating contaminated metal with nitric acid has been shown to significantly enhance the decontamination performance. When compared to proposed baseline washout procedures at Sellafield, the only change to the process is the applied electrical waveform which is temporary and controllable. The current causes dissolution of the metal surface being decontaminated which leads to activity transferring from the plant item into the nitric acid effluent stream. This innovation has the potential to transform the POCO process and allow a nitric acid based washout approach to deliver POCO quickly and cost effectively within the existing infrastructure. Several devices have now been designed that incorporate this EASD{sup TM} technology and enable the decontamination process to be applied to a range items commonly identified as being contaminated during nuclear decommissioning programmes such as pipework, tanks and hotspots of walls/floors. Inactive and active laboratory-scale testing has been completed using both simulated and 'real-life' contaminated (low level waste) metallic items retrieved from different nuclear sites. Active trials demonstrated that contaminated items could be treated to free release levels within minutes. Engineering scale tests are currently being performed, with guidance from Sellafield's system engineers and plant managers, to provide the necessary re-assurance the designed devices could be successfully deployed in an on-plant scenario. The final stage of this development work is to carry out an active demonstration of the decontamination technology on Thorp at Sellafield. This paper aims to highlight progress made to date with the EASD{sup TM} technology and, more specifically, the development of an in-situ decontamination device for deployment within radioactive pipework. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Control and automation of the Pegasus multi-point Thomson scattering system

A new control system for the Pegasus Thomson scattering diagnostic has recently been deployed to automate the laser operation, data collection process, and interface with the system-wide Pegasus control code. Automation has been extended to areas outside of data collection, such as manipulation of beamline cameras and remotely controlled turning mirror actuators to enable intra-shot beam alignment. Additionally, the system has been upgraded with a set of fast (∼1 ms) mechanical shutters to mitigate contamination from background light. Modification and automation of the Thomson system have improved both data quality and diagnostic reliability.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Innovative use of Cr(VI) plume depictions and pump-and-treat capture analysis to estimate risks of contaminant discharge to surface water at Hanford reactor areas - 15315

The Hanford Site nuclear reactor operations required large quantities of high-quality cooling water, which was treated with chemicals including sodium dichromate dihydrate for corrosion control. Cooling water leakage, as well as intentional discharge of cooling water to ground during upset conditions, produced extensive groundwater recharge mounds consisting largely of contaminated cooling water and resulted in wide distribution of hexavalent chromium (Cr[VI]) contamination in the unconfined aquifer. The 2013 Cr(VI) groundwater plumes in the 100 Areas cover approximately 6 km{sup 2} (1500 acres), primarily in the 100-HR-3 and 100-KR-4 groundwater operable units (OUs). The Columbia River is a groundwater discharge boundary; where the plumes are adjacent to the Columbia River there remains a potential to discharge Cr(VI) to the river at concentrations above water quality criteria. The pump-and-treat systems along the River Corridor are operating with two main goals: 1) protection of the Columbia River, and 2) recovery of contaminant mass. An evaluation of the effectiveness of the pump-and-treat systems was needed to determine if the Columbia River was protected from contamination, and also to determine where additional system modifications may be needed. In response to this need, a technique for assessing the river protection was developed which takes into consideration seasonal migration of the plume and hydraulic performance of the operating well fields. Groundwater contaminant plume maps are generated across the Hanford Site on an annual basis. The assessment technique overlays the annual plume and the capture efficiency maps for the various pump and treat systems. The river protection analysis technique was prepared for use at the Hanford site and is described in detail in M.J. Tonkin, 2013. Interpolated capture frequency maps, based on mapping dynamic water level observed in observation wells and derived water levels in the vicinity of extraction and injection wells, are developed initially. Second, simulated capture frequency maps are developed, based on transport modelling results. Both interpolated and simulated capture frequency maps are based on operation of the systems over a full year. These two capture maps are then overlaid on the plume distribution maps for inspection of the relative orientation of the contaminant plumes with the capture frequency. To quantify the relative degree of protection of the river from discharges of Cr(VI) (and conversely, the degree of threat) at any particular location, a systematic method of evaluating and mapping the plume/capture relationship was developed. By comparing the spatial relationship between contaminant plumes and hydraulic capture frequency, an index of relative protectiveness is developed and the results posted on the combined plume/capture plan view map. Areas exhibiting lesser degrees of river protection are identified for remedial process optimization actions to control plumes and prevent continuing discharge of Cr(VI) to the river. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Are light curve classification metrics good proxies for SN Ia cosmological constraining power?

Context. When selecting a light curve classifier for use as part of a photometric supernova Ia (SN Ia) cosmological analysis, it is common to make decisions based on metrics of classification performance, such as the contamination within the photometrically classified SN Ia sample, rather than a measure of cosmological constraining power. If the former is an appropriate proxy for the latter, this practice would eliminate the computational expense of a full cosmology forecast in the analysis pipeline design process. Aims. This study tests the assumption that light curve classification metrics are an appropriate proxy for cosmology metrics. Methods. We emulated photometric SN Ia cosmology light curve samples with controlled contamination rates of individual contaminant classes and evaluated each of them under a set of classification metrics. We then derived cosmological parameter constraints from all samples under two common analysis approaches and quantified the impact of contamination by each contaminant class on the resulting cosmological parameter estimates. Results. We observe that cosmology metrics are sensitive to both the contamination rate and the class of the contaminating population, whereas the classification metrics are shown to be insensitive to the latter. Conclusions. Based on these findings, we discourage any exclusive reliance on light curve classification-based metrics for analysis design decisions, which (counterintuitively) include but are not limited to the classifier choice. Instead, we recommend optimising science analysis pipeline design choices using a metric of the information gained about the physical parameters of interest.

79 ASTRONOMY AND ASTROPHYSICS↗

Waste Management 2016 300-296 Soil Remediation Project. Developing Innovative Solutions to Overcome Design Challenges - 16229

The 324 Building is a non-reactor, Hazard Category 2, Nuclear Facility located in the 300 Area of the U.S. Department of Energy (DOE) Hanford Site, located near Richland, Washington. The building is undergoing deactivation, decommissioning, decontamination, and demolition by Washington Closure Hanford (WCH) under the River Corridor Closure Contract. During the Cold War Era, the facility was used for chemical and radionuclide processing associated with nuclear weapons production. Historical records indicate that in October 1986, approximately 516 L of a concentrated liquid waste stream containing cesium-137 and strontium-90 was spilled onto the floor of B-Cell. B-Cell is located in the Radiochemical Engineering Complex (REC) of the 324 Building. It is estimated that this spill contained approximately 1.3 million curies of radioactivity. Some fraction of the spill was recovered; however, an unknown quantity was released into the soil beneath the 324 Building through an undetected breach in the B-Cell floor sump. Unknown quantities of water were used after the spill to wash items contained in B-Cell. When B-Cell was taken out of service, wastes being removed from B-Cell were placed in Grout Containers (GCs) and stabilized using a particulate grout. In the course of grouting activities, grout was spilled on the floor of B-Cell, eventually filling the sump. The primary scope of this project is to design, test, and implement a process capable of removing and isolating contaminated soil below B-Cell. This remediation effort has been divided into two removal zones identified as the primary and secondary removal zones. Based on current assumptions and estimates, the primary phase of this cleanup effort will remove roughly 156.45 m{sup 3} of highly contaminated materials. B-Cell debris, particulate grout, and the floor slab and liner comprise about 21.24 m{sup 3} of the total. The remaining 135.21 m3 will be contaminated soil. All of this material must be disposed of within the existing 324 Facility or packaged for disposal at the Hanford site. The objective of this project is to remove the B-Cell floor and highly contaminated underlying soils from beneath B-Cell using remote equipment to reduce the radiation levels and allow open-air excavation for the remaining contaminated soil. The challenges and subsequent technological solutions encountered from the Conceptual Design phase to the 100% Design Issued For Fabrication/Issued For Construction (IFF/IFC) consisted in part of the following: Design an efficient remote cleanup strategy for highly contaminated Low Level Waste (LLW) given the unknowns. Maintain contamination control down to 18 feet below B-Cell in a highly radioactive environment (12,870 R/Hr Cs-137 and Sr-90). Perform facility upgrades to enable remediation process implementation while maintaining integrity of pre-existing facility structure and license. Designing first-of-a-kind remote equipment for use in high radiation areas. Developing first-of-a-kind operations sequences for remote equipment while minimizing usage of original facility equipment (i.e. cranes, manipulators, etc.). Conducting time-motion studies of operations sequences to maximize efficiency while keeping dose As Low As Reasonably Achievable (ALARA) in a high dose environment. Construction of a full scale mockup to conduct performance testing in a clean environment and train the work force to operate the equipment. Overcoming scope, budget and schedule constraints associated with a fast-track DOE project. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Pathways towards Valid Data: Assessment of Optimized Packaging Strategies for Core Components - 18158

Every company that runs nuclear power plants needs a strategy for disposal of the radioactive waste and for decommissioning and dismantling of its nuclear reactors. The dismantling progress of several German NPPs is already in an advanced stage. Hence, particularly highly activated core components have to be disposed of as radioactive waste. Nevertheless, this is also an issue during the operating phase due to necessary replacements of highly activated core components such as PWR control elements or RPV heads. The conditioning of waste packages for final disposal in the German repository Konrad requires the core components to be dismantled, packaged and dried. Therefore, a qualified packaging strategy is needed to ensure a reliable compliance with the protection objectives of the repository and an optimized utilization of prescribed contingents. Exemplified by PWR control elements a pathway is drawn how to perform an optimized packaging strategy in compliance with the protection objectives of the repository based on only few relevant radionuclides derived from activation calculations. For these calculations, particularly the influence of trace impurities in the materials has to be considered and their amounts have to be verified or analyzed by sampling. Furthermore, low nuclide activities from contamination cannot simply be neglected because some of them are obliged to be declared or their radiological relevance has not been sufficiently determined. Due to various influence factors, qualified sampling strategies need carefully considered concepts. Therefore, detailed planning facilitates the qualification and approval process and reduces the costs for analyses and the number of containers. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Identifying geochemical hot moments and their controls on a contaminated river floodplain system using wavelet and entropy approaches

Geochemical hot moments are defined here as short periods of time that are associated with disproportionally high levels of concentrations (biogeochemically-driven or transport-related) relative to longer intervening time periods. We used entropy and wavelet techniques to identify temporal variability in geochemical constituents and their controls along three transects within a contaminated floodplain system near Rifle CO. Results indicated that transport-dominated hot moments drove overall geochemical processing in the contaminated groundwater and seep zones. These hot moments were associated with seasonal hydrologic variability (~4 months) in the contaminated aquifer and with annual hydrologic cycle and residence times in the seep zone. Hot moments associated with a naturally reduced zone within the aquifer were found to be biogeochemically-driven, with a different dominant frequency (~3 months) and no correlation to hydrologic or weather variations, in contrast to what is observed in other regions of the floodplain.

54 ENVIRONMENTAL SCIENCES↗

Morphological and crystallographic controls in the replacement of calcite and aragonite by cerussite and otavite

Mineral replacement reactions are essential solid-fluid interactions in natural and industrial processes including metasomatism, diagenesis, and metamorphism, and sequestration of metal toxins from polluted water. In this paper, we explore the morphological evolution of aragonite and calcite (polymorphs of CaCO 3 ) during their replacement by cerussite (PbCO 3 , an isomorph of aragonite) and otavite (CdCO 3 , an isomorph of calcite) in acidic aqueous solutions (with initial pH 3 to 4). Observations using scanning electron and synchrotron-based transmission X-ray microscopies reveal the formation of pseudomorphic shells (~5 to 10 µm thick) of cerussite and otavite when Pb 2+ is reacted with either calcite or aragonite and when Cd 2+ is reacted with aragonite. The formation of pore space at the substrate-precipitate interface and within the precipitate is found to be key in promoting chemical exchange between the dissolving phase and the solution. In contrast, minimal reactivity is observed when Cd 2+ is reacted with calcite. These results demonstrate that the lack of substrate-precipitate epitaxy (either because of inconsistent symmetries or due to large lattice constant mismatch) is a critical factor that enables replacement of primary CaCO 3 minerals by Pb- or Cd-bearing secondary minerals while the presence of epitaxial relationships inhibit those reactions. When calcite and aragonite coexist, the replacement of aragonite by cerussite and otavite occurs preferentially over calcite. We postulate that the relative reactivity of calcite and aragonite to Pb 2+ and Cd 2+ can be determined by interplay between morphological and crystallographic properties of CaCO 3 materials as well as solution chemistry. Our results demonstrate that coupled dissolution-precipitation processes of carbonate minerals play an important role in controlling mobility and bioavailability of lead and cadmium in environments where acidic metal-contaminated water interacts with carbonate-rich rocks and soils.

58 GEOSCIENCES↗

Scaling Effects of Cr(VI) Reduction Kinetics. The Role of Geochemical Heterogeneity

The natural subsurface is highly heterogeneous with minerals distributed in different spatial patterns. Fundamental understanding of how mineral spatial distribution patterns regulate sorption process is important for predicting the transport and fate of chemicals. Existing studies about the sorption was carried out in well-mixed batch reactors or uniformly packed columns, with few data available on the effects of spatial heterogeneities. As a result, there is a lack of data and understanding on how spatial heterogeneities control sorption processes. In this project, we aim to understand and develop modeling capabilities to predict the sorption of Cr(VI), an omnipresent contaminant in natural systems due to its natural occurrence and industrial utilization. We systematically examine the role of spatial patterns of illite, a common clay, in determining the extent of transport limitation and scaling effects associated with Cr(VI) sorption capacity and kinetics using column experiments and reactive transport modeling. Our results showed that the sorbed mass and rates can differ by an order of magnitude due to of the illite spatial heterogeneities and transport limitation. With constraints from data, we also developed the capabilities of modeling Cr(VI) in heterogeneous media. The developed model is then utilized to understand the general principles that govern the relationship between sorption and connectivity, a key measure of the spatial pattern characteristics. This correlation can be used to estimate Cr(VI) sorption characteristics in heterogeneous porous media. Insights gained here bridge gaps between laboratory and field application in hydrogeology and geochemical field, and advance predictive understanding of reactive transport processes in the natural heterogeneous subsurface. We believe that these findings will be of interest to a large number of environmental geochemists and engineers, hydrogeologists, and those interested in contaminant fate and transport, water quality and water composition, and natural attenuation processes in natural systems.

58 GEOSCIENCES↗

Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells

Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. - Highlights: • A simple, closed-channel-based, semi-automatic culture system is proposed. • Uniform cell aggregate formation and culture is realized in microwell structure. • Functional islet cells are successfully induced following 30-plus-day protocol. • System requires no daily medium replacement and reduces contamination risk.

60 APPLIED LIFE SCIENCES↗

Study of an unrefined humate solution as a possible remediation method for groundwater contamination - 15698

Savannah River Site (SRS) was one of the major nuclear processing facilities in the U.S. where plutonium was produced during the Cold War. Approximately 1.8 billion gallons of low level acidic waste solution containing radionuclides and dissolved metals were discharged to a series of unlined seepage basins at the F/H Area. Uranium is a key contaminant of concern in the basin's groundwater. It is migrating into the groundwater creating an acidic plume with a pH between 3-5.5. Groundwater Actions: The pump-and-treat water treatment unit was designed and built in 1997 to remove metals and radionuclides. In 2004 a hybrid funnel-and-gate system was constructed to create a treatment zone in which the acidic nature of the contaminated sediments could be reversed. Huma-K is an organic fertilizer that comes from the alkaline extraction of leonardite (a low-rank coal). Huma-K has a high content of humic substances which consist of complex organic compounds formed by the decomposition of plant and animal tissue. The objective of this study is to determine if a low cost unrefined humate solution (Huma-K) can be used to facilitate uranium adsorption to control its mobility in acidic groundwater. This study can assist in evaluating whether Huma-K can be used as an in situ amendment for the remediation of groundwater contaminated with uranium. Sediments from FAW-1 70-90 ft were used in this experiment. Sediments were disaggregated and sieved to a particle size of . 2 mm. Concentrations in the range 10-500 ppm were tested. Once all the components were added, pH was adjusted to 4. Samples were vortex mixed and placed on a shaker table at 100 RPM for a period of 24 hours in order to reach the adsorption equilibrium. Once the adsorption equilibrate was reached, samples were centrifuged at 2700 RPM to separate the liquid and the solid phase. The liquid fraction was analyzed using a Thermo Scientific Genesys 10S UV-Vis spectrophotometer. The same experimental design was used as explained above. A known concentration of Huma-K was used to study the pH effect on the sorption process. pH values were measured at the beginning and at the end of the experiment. Spectra of Huma-K show a featureless character. Absorbance of light increases as the wavelength is decreased. Measurements were done at 254 nm due to the presence of aromatic rings. Apparently there are two sorption processes. The first one follows the same trend as the Langmuir isotherm up to 250 ppm. The second sorption process occurs after all the binding sites in the sediments have been occupied. Precipitation decreases as the pH is increased. Based on the sorption isotherm, humic substances apparently bind first to the binding sites of the sediments particles. Once all the binding sites have been occupied, other sorption processes may start to dominate. Sorption is affected by pH. Higher sorption is seen at low pH values. Increased precipitation at low pH values is probably due to the neutralization of surface charges of humic molecules making them agglomerate.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗