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At least 1,243 records · Page 69
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Synthesis and Characterization of Copolymers and Nanocomposites from Limonene, Styrene and Organomodified-Clay Using Ultrasonic Assisted Method

In the present work, we report a simple synthesis method for preparation of copolymers and nanocomposites from limonene and styrene using clay as a catalyst. The copolymerization reaction is carried out by using a proton exchanged clay as a catalyst called Mag-H+. The effect of temperature, reaction time and amount of catalyst were studied, and the obtained copolymer structure (lim-co-sty) is characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (1H-NMR) and differential scanning calorimetry (DSC). The molecular weight of the obtained copolymer is determined by gel permeation chromatography (GPC) and is about 4500 g·mol−1. The (lim-co-sty/Mag 1%, 3%, 7% and 10% by weight of clay) nanocomposites were prepared through polymer/clay mixture in solution method using ultrasonic irradiation, in the presence of Mag-CTA+ as green nano-reinforcing filler. The Mag-CTA+ is organophilic silicate clay prepared through a direct exchange process, using cetyltrimethylammonuim bromide (CTAB). The prepared lim-co-sty/Mag nanocomposites have been extensively characterized by FT-IR spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and transmission electronic microscopy (TEM). TEM analysis confirms the results obtained by XRD and clearly show that the obtained nanocomposites are partially exfoliated for the lower amount of clay (1% and 3% wt) and intercalated for higher amounts of clay (7% and 10% wt). Moreover, thermogravimetric analysis (TGA) indicated an enhancement of thermal stability of nanocomposites compared with the pure copolymer.

Derdar, Hodhaifa↗
DOE OSTI2023

Characterizing Current THD’s Dependency on Solar Irradiance and Supraharmonics Profiling for a Grid-Tied Photovoltaic Power Plant

The rapidly increasing distributed energy resources (DERs) in power systems are now getting interconnected to set community grid structures, where power quality will be a major concern. The grid-to-grid (G2G) bidirectional power transfer among the distribution microgrid will not be considered commercially feasible unless the upstream harmonics are under the limits. The aggregation of such harmonics, measured as total harmonic distortion (THD), is feared to be beyond tolerable limits with the progression of rooftop grid-tied PV-like installations. Hence, this THD needs to be characterized with DER generation end variables. In this work, the photovoltaic (PV) DERs’ dependency on environment variables such as irradiance was profiled in the context of generating and injecting harmonics into the grid. A mathematical model of a grid-tied three-phase PV DER was developed as part of this correlation characterization, matching the fundamental unit structure of a 1.4 MW solar canopy located on the Florida International University (FIU) Miami campus. To determine the qualitative association with produced THD patterns, the model was evaluated with various irradiance settings. A real-time digital simulation (RTDS) platform was used to verify it. Following this confirmation, sets of data from power quality meters at the point of common coupling and FIU field sensors were utilized to validate further the correlation model. The results showed that the grid current’s THD exhibited a high correlation with the irradiance profile and its variation over time. The early morning and late afternoon periods of the day, associated with a low irradiance, constantly had higher harmonics generated from the PV DER. The midday THD was rather rational with partial shadings, hence a geolocation-dependent factor. These findings were verified by an RTDS and validated by real field data. In quantifying the THD injected by a single DER at a high-frequency (2–150 kHz) supraharmonics (SH) level, a 3% peak increment in magnitude was observed from the high-fixed to the low-fixed irradiance profile. The correlation characteristics depicted that the hybrid microgrid suffered from a daytime-dependent harmonic insertion from the grid-tied DER. This is a global problem unless specific measures are taken to mitigate the harmonics. The electrically notorious higher-frequency SH was found to increase proportionally. The G2G power transfer can be limited because of the higher THD in the early morning and late afternoon, which will also worsen because the numbers of grid-tied PV DERs (i.e., rooftop solar and industrial solar) are likely to increase rapidly soon. The community grid structure can thus have a controlled harmonics filtration setup purposefully designed to address the findings of this work, which also fall within the scope of our future research.

14 SOLAR ENERGY↗
DOE OSTI2023

Comprehensive Genetic Characterization of Four Novel HIV-1 Circulating Recombinant Forms (CRF129_56G, CRF130_A1B, CRF131_A1B, and CRF138_cpx): Insights from Molecular Epidemiology in Cyprus

Molecular investigations of the HIV-1 pol region (2253–5250 in the HXB2 genome) were conducted on sequences obtained from 331 individuals infected with HIV-1 in Cyprus between 2017 and 2021. This study unveiled four distinct HIV-1 putative transmission clusters, encompassing 19 previously unidentified HIV-1 recombinants. These recombinants, each comprising eight, three, four, and four sequences, respectively, did not align with previously established Circulating Recombinant Forms (CRFs). To characterize these novel HIV-1 recombinants, near-full-length genome sequences were successfully obtained for 16 of the 19 recombinants (790–8795 in the HXB2 genome) using an in-house-developed RT-PCR assay. Phylogenetic analyses, employing MEGAX and Cluster-Picker, along with confirmatory neighbor-joining tree analyses of subregions, were conducted to identify distinct clusters and determine subtypes. The uniqueness of the HIV-1 recombinants was evident in their exclusive clustering within generated maximum likelihood trees. Recombination analyses highlighted the distinct chimeric nature of these recombinants, with consistent mosaic patterns observed across all sequences within each of the four putative transmission clusters. Conclusive genetic characterization identified four novel HIV-1 CRFs: CRF129_56G, CRF130_A1B, CRF131_A1B, and CRF138_cpx. CRF129_56G exhibited two recombination breakpoints and three fragments of subtypes CRF56_cpx and G. Both CRF130_A1B and CRF131_A1B featured seven recombination breakpoints and eight fragments of subtypes A1 and B. CRF138_cpx displayed five recombination breakpoints and six fragments of subtypes CRF22_01A1 and F2, along with an unclassified fragment. Additional BLAST analyses identified a Unique Recombinant Form (URF) of CRF138_cpx with three additional recombination sites, involving subtype F2, a fragment of unknown subtype origin, and CRF138_cpx. Post-identification, all putative transmission clusters remained active, with CRF130_A1B, CRF131_A1B, and CRF138_cpx clusters exhibiting further growth. Furthermore, international connections were identified through BLAST analyses, linking one sequence from the USA to the CRF130_A1B strain, and three sequences from Belgium and Cameroon to the CRF138_cpx strain. This study contributes valuable insights into the dynamic landscape of HIV-1 diversity and transmission patterns, emphasizing the need for ongoing molecular surveillance and global collaboration in tracking emerging viral variants.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2020

ARES. II. Characterizing the Hot Jupiters WASP-127 b, WASP-79 b, and WASP-62b with the Hubble Space Telescope

This paper presents the atmospheric characterization of three large, gaseous planets: WASP-127 b, WASP-79 b, and WASP-62 b. We analyzed spectroscopic data obtained with the G141 grism (1.088–1.68 μm) of the Wide Field Camera 3 on board the Hubble Space Telescope using the Iraclis pipeline and the TauREx3 retrieval code, both of which are publicly available. For WASP-127 b, which is the least dense planet discovered so far and is located in the short-period Neptune desert, our retrieval results found strong water absorption corresponding to an abundance of log(H{sub 2}O) = −2.71{sub −1.05}{sup +0.78} and absorption compatible with an iron hydride abundance of log(FeH) = −5.25{sub −1.10}{sup +0.88}, with an extended cloudy atmosphere. We also detected water vapor in the atmospheres of WASP-79 b and WASP-62 b, with best-fit models indicating the presence of iron hydride, too. We used the Atmospheric Detectability Index as well as Bayesian log evidence to quantify the strength of the detection and compared our results to the hot Jupiter population study by Tsiaras et al. While all the planets studied here are suitable targets for characterization with upcoming facilities such as the James Webb Space Telescope and Ariel, WASP-127 b is of particular interest due to its low density, and a thorough atmospheric study would develop our understanding of planet formation and migration.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

The First Habitable-zone Earth-sized Planet from TESS. III. Climate States and Characterization Prospects for TOI-700 d

We present self-consistent three-dimensional climate simulations of possible habitable states for the newly discovered habitable-zone Earth-sized planet TOI-700 d. We explore a variety of atmospheric compositions, pressures, and rotation states for both ocean-covered and completely desiccated planets in order to assess the planet’s potential for habitability. For all 20 of our simulated cases, we use our climate model outputs to synthesize transmission spectra, combined-light spectra, and integrated broadband phase curves. These climatologically informed observables will help the community assess the technological capabilities necessary for future characterization of this planet—as well as similar transiting planets discovered in the future—and will provide a guide for distinguishing possible climate states if one day we do obtain sensitive spectral observations of a habitable planet around an M star. We find that TOI-700 d is a strong candidate for a habitable world and can potentially maintain temperate surface conditions under a wide variety of atmospheric compositions. Unfortunately, the spectral feature depths from the resulting transmission spectra and the peak flux and variations from our synthesized phase curves for TOI-700 d do not exceed 10 ppm. This will likely prohibit the James Webb Space Telescope from characterizing its atmosphere; however, this motivates the community to invest in future instrumentation that perhaps can one day reveal the true nature of TOI-700 d and to continue to search for similar planets around less distant stars.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Prospects for Characterizing the Haziest Sub-Neptune Exoplanets with High-resolution Spectroscopy

Observations to characterize planets larger than Earth but smaller than Neptune have led to largely inconclusive interpretations at low spectral resolution due to hazes or clouds that obscure molecular features in their spectra. However, here we show that high-resolution spectroscopy (R ∼ 25,000–100,000) enables one to probe the regions in these atmospheres above the clouds where the cores of the strongest spectral lines are formed. We present models of transmission spectra for a suite of GJ 1214b–like planets with thick photochemical hazes covering 1–5 μm at a range of resolutions relevant to current and future ground-based spectrographs. Furthermore, we compare the utility of the cross-correlation function that is typically used with a more formal likelihood-based approach, finding that only the likelihood-based method is sensitive to the presence of haze opacity. We calculate the signal-to-noise ratio (S/N) of these spectra, including telluric contamination, Required to robustly detect a host of molecules such as CO, CO{sub 2}, H{sub 2}O, and CH{sub 4} and photochemical products like HCN as a function of wavelength range and spectral resolution. Spectra in the M band require the lowest S/N{sub res} to detect multiple molecules simultaneously. CH{sub 4} is only observable for the coolest models (T {sub eff} = 412 K) and only in the L band. We quantitatively assess how these requirements compare to what is achievable with current and future instruments, demonstrating that characterization of small cool worlds with ground-based high-resolution spectroscopy is well within reach.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Precise Transit and Radial-velocity Characterization of a Resonant Pair: The Warm Jupiter TOI-216c and Eccentric Warm Neptune TOI-216b

TOI-216 hosts a pair of warm, large exoplanets discovered by the TESS mission. These planets were found to be in or near the 2:1 resonance, and both of them exhibit transit timing variations (TTVs). Precise characterization of the planets’ masses and radii, orbital properties, and resonant behavior can test theories for the origins of planets orbiting close to their stars. Previous characterization of the system using the first six sectors of TESS data suffered from a degeneracy between planet mass and orbital eccentricity. Radial-velocity measurements using HARPS, FEROS, and the Planet Finder Spectrograph break that degeneracy, and an expanded TTV baseline from TESS and an ongoing ground-based transit observing campaign increase the precision of the mass and eccentricity measurements. We determine that TOI-216c is a warm Jupiter, TOI-216b is an eccentric warm Neptune, and that they librate in 2:1 resonance with a moderate libration amplitude of 60{sub −2}{sup +2} deg, a small but significant free eccentricity of 0.0222{sub −0.0003}{sup +0.0005} for TOI-216b, and a small but significant mutual inclination of 1.°2–3.°9 (95% confidence interval). The libration amplitude, free eccentricity, and mutual inclination imply a disturbance of TOI-216b before or after resonance capture, perhaps by an undetected third planet.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2022

Characterizing and Mitigating Intraday Variability: Reconstructing Source Structure in Accreting Black Holes with mm-VLBI

The extraordinary physical resolution afforded by the Event Horizon Telescope has opened a window onto the astrophysical phenomena unfolding on horizon scales in two known black holes, M87* and Sgr A*. However, with this leap in resolution has come a new set of practical complications. Sgr A* exhibits intraday variability that violates the assumptions underlying Earth aperture synthesis, limiting traditional image reconstruction methods to short timescales and data sets with very sparse (u, v) coverage. We present a new set of tools to detect and mitigate this variability. We develop a data-driven, model-agnostic procedure to detect and characterize the spatial structure of intraday variability. This method is calibrated against a large set of mock data sets, producing an empirical estimator of the spatial power spectrum of the brightness fluctuations. We present a novel Bayesian noise modeling algorithm that simultaneously reconstructs an average image and statistical measure of the fluctuations about it using a parameterized form for the excess variance in the complex visibilities not otherwise explained by the statistical errors. These methods are validated using a variety of simulated data, including general relativistic magnetohydrodynamic simulations appropriate for Sgr A* and M87*. We find that the reconstructed source structure and variability are robust to changes in the underlying image model. We apply these methods to the 2017 EHT observations of M87*, finding evidence for variability across the EHT observing campaign. The variability mitigation strategies presented are widely applicable to very long baseline interferometry observations of variable sources generally, for which they provide a data-informed averaging procedure and natural characterization of inter-epoch image consistency.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Characterization of CdZnTeSe Nuclear Detector Chemically Etched in Bromine Methanol

Semiconductor nuclear radiation detectors made from tertiary and quaternary compounds of cadmium telluride (CdTe) can operate at room temperature without cryogenic cooling. One of such materials that have become of great interest is cadmium zinc telluride selenide (CdZnTeSe). Compared to other CdTe-based materials, such as cadmium zinc telluride (CdZnTe), CdZnTeSe can be grown with much less Te inclusions and sub-grain boundary networks. Chemical etching is often used to smoothen wafer surfaces during detector fabrication. This paper presents the characterization of CdZnTeSe that is chemically etched using bromine methanol solution. Infrared imaging shows that the wafer has no sub-grain boundary networks that often limit detector performance. The current-voltage (I-V) characterization experiment gave a resistivity of 4.6 × 10 10 Ω-cm for the sample. The I-V curve was linear in the ±10 to ±50 volts range. An energy resolution of 7.2% was recorded at 100 V for the 59.6-keV gamma line of 241 Am.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2022

Micro-spectroscopic and freezing characterization of ice-nucleating particles collected in the marine boundary layer in the eastern North Atlantic

Abstract. Formation of atmospheric ice plays a crucial role in the microphysical evolution of mixed-phase and cirrus clouds and thus climate. How aerosol particles impact ice crystal formation by acting as ice-nucleating particles (INPs) is a subject of intense research activities. To improve understanding of atmospheric INPs, we examined daytime and nighttime particles collected during the Aerosol and Cloud Experiments in the Eastern North Atlantic (ACE-ENA) field campaign conducted in summer 2017. Collected particles, representative of a remote marine environment, were investigated for their propensity to serve as INPs in the immersion freezing (IMF) and deposition ice nucleation (DIN) modes. The particle population was characterized by chemical imaging techniques such as computer-controlled scanning electron microscopy with energy-dispersive X-ray analysis (CCSEM/EDX) and scanning transmission X-ray microscopy with near-edge X-ray absorption fine-structure spectroscopy (STXM/NEXAFS). Four major particle-type classes were identified where internally mixed inorganic–organic particles make up the majority of the analyzed particles. Following ice nucleation experiments, individual INPs were identified and characterized by SEM/EDX. The identified INP types belong to the major particle-type classes consisting of fresh sea salt with organics or processed sea salt containing dust and sulfur with organics. Ice nucleation experiments show IMF events at temperatures as low as 231 K, including the subsaturated regime. DIN events were observed at lower temperatures of 210 to 231 K. IMF and DIN observations were analyzed with regard to activated INP fraction, ice-nucleation active site (INAS) densities, and a water activity-based immersion freezing model (ABIFM) yielding heterogeneous ice nucleation rate coefficients. Observed IMF and DIN events of ice formation and corresponding derived freezing rates demonstrate that the marine boundary layer aerosol particles can serve as INPs under typical mixed-phase and cirrus cloud conditions. The derived IMF and DIN parameterizations allow for implementation in cloud and climate models to evaluate predictive effects of atmospheric ice crystal formation.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Quantitative chemical assay of nanogram-level particulate matter using aerosol mass spectrometry: characterization of particles collected from uncrewed atmospheric measurement platforms

Abstract. Aerosol generation techniques have expanded the utility of aerosol mass spectrometry (AMS) for offline chemical analysis of airborne particles and droplets. However, standard aerosolization techniques require relatively large liquid volumes (e.g., several milliliters) and high sample masses that limit their utility. Here we report the development and characterization of a micronebulization AMS (MN-AMS) technique that requires as low as 10 µL of sample and can provide the quantification of the nanogram level of organic and inorganic substances via the usage of an isotopically labeled internal standard (34SO42-). Using standard solutions, the detection limits for this technique were determined at 0.19, 0.75, and 2.2 ng for sulfate, nitrate, and organics, respectively. The analytical recoveries for these species are 104 %, 87 %, and 94 %, respectively. This MN-AMS technique was applied successfully to analyze filter and impactor samples collected using miniature particulate matter (PM) samplers deployable on uncrewed atmospheric measurement platforms, such as uncrewed aerial systems (UASs) and tethered balloon systems (TBSs). Chemical composition of PM samples collected from a UAS field campaign conducted at the Department of Energy's (DOE) Southern Great Plains (SGP) observatory was characterized. The offline MN-AMS data compared well with the in situ PM composition measured by a co-located aerosol chemical speciation monitor (ACSM). In addition, the MN-AMS and ion chromatography (IC) agreed well for measurements of sulfate and nitrate concentrations in the PM extracts. This study demonstrates the utility of combining MN-AMS with uncrewed measurement platforms to provide quantitative measurements of ambient PM composition.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Tools for uranium characterization in carbonate samples: case studies of natural U–Pb geochronology reference materials

Laser ablation U–Pb analyses of carbonate (LAcarb) samples has greatly expanded the potential for U–Pb dating to a variety of carbonate-producing settings. Carbonates that were previously considered impossible to date using isotope dilution methods may preserve radiogenic domains that can be dated using spatially resolved laser ablation geochronology techniques. Work is ongoing to identify reference materials and to consider best practices for LAcarb. In this study we apply standard and emerging characterization tool sets on three natural samples with the dual goal of enhancing the study of carbonates and establishing a new set of well-characterized natural reference materials for LAcarb studies. We start with the existing carbonate reference material WC-1 from the Permian Reef Complex of Texas, building on the published description to offer a deeper look at U and associated trace elements. We consider a tufa sample from the Miocene Barstow Formation of the Mojave Block, California, as a possible secondary calcite reference material due to its well-behaved U–Pb systematics. There are currently no natural dolomite standards. We present an unusual dolomite sample with very well-behaved U–Pb systematics from the Miocene of the Turkana Basin of Kenya as a possible dolomite reference material for LAcarb dating. In addition to using X-ray fluorescence (XRF) mapping and spectroscopy to better understand U in these natural samples, we have analyzed multiple aliquots of each of them for 87 Sr/ 86 Sr by thermal ionization mass spectrometry (TIMS). The Sr isotope compositions are analytically homogeneous within petrographically homogeneous regions of all three samples, and thus these materials could be used as Sr isotope standards as well. While not part of the current contribution, this combination could streamline simultaneous LA analyses of 87 Sr/ 86 Sr and U–Pb geochronology.

36 MATERIALS SCIENCE↗
DOE OSTI2021

WRF-simulated low-level jets over Iowa: characterization and sensitivity studies

Abstract. Output from 6 months of high-resolution simulations with the Weather Research and Forecasting (WRF) model are analyzed to characterize local low-level jets (LLJs) over Iowa for winter and spring in the contemporary climate. Low-level jets affect rotor plane aerodynamic loading, turbine structural loading and turbine performance, and thus accurate characterization and identification are pertinent. Analyses using a detection algorithm wherein the wind speed above and below the jet maximum must be below 80 % of the jet wind speed within a vertical window of approximately 20–530 m a.g.l. (above ground level) indicate the presence of an LLJ in at least one of the 14 700 4 km×4 km grid cells over Iowa on 98 % of nights. Nocturnal LLJs are most frequently associated with stable stratification and low turbulent kinetic energy (TKE) and hence are more frequent during the winter months. The spatiotemporal mean LLJ maximum (jet core) wind speed is 9.55 m s−1, and the mean height is 182 m. Locations of high LLJ frequency and duration across the state are seasonally varying, with a mean duration of 3.5 h. The highest frequency occurs in the topographically complex northwest of the state in winter and in the flatter northeast of the state in spring. Sensitivity of LLJ characteristics to the (i) LLJ definition and (ii) vertical resolution at which the WRF output is sampled is examined. LLJ definitions commonly used in the literature are considered in the first sensitivity analysis. These sensitivity analyses indicate that LLJ characteristics are highly variable with definition. Use of different definitions identifies both different frequencies of LLJs and different LLJ events. Further, when the model output is down-sampled to lower vertical resolution, the mean jet core wind speed height decreases, but spatial distributions of regions of high frequency and duration are conserved. Implementation of a polynomial interpolation to extrapolate down-sampled output to full-resolution results in reduced sensitivity of LLJ characteristics to down-sampling.

17 WIND ENERGY↗
DOE OSTI2023

Advancing uncertainty characterization for understanding projected water scarcity in multi-sector, multi-actor river basins across scales.

Invited Talk at AGU Fall Meeting 2023 Modeling how human institutions and infrastructure interact with the water cycle is essential to better understand the vulnerability, and resilience of water resources systems from the local to the global scale. This is especially true when investigating multi-sector, multi-actor responses to the effects of long-term changes and short-term shocks. It is also well recognized that uncertainty present throughout the modeling cycle (e.g., in data, functional relations, and model coupling approaches) limits our ability to trace the interactive dynamics of human-water systems, as well as to quantify their implications for management and planning. Systems with large numbers of diverse stakeholders further compound this challenge, as uncertain drivers and complex dynamics might have very disparate effects on water users. The work presented is conducted through the Integrated Multisector Multiscale Modeling (IM3) Science Focus Area, which explores how human and natural systems co-evolve in response to change. Through this multi-year effort, we have developed exploratory modeling methods to better characterize how the uncertain human and natural drivers of water scarcity yield consequential vulnerabilities in institutionally complex, multi-sectoral systems. This talk will specifically present on a series of uncertainty characterization experiments performed in the Upper Colorado River Basin, a sub-basin of the Colorado with thousands of water users. These complementary and systematic experiments are aimed at understanding: How are various uncertain stressors (e.g., climate change, demand growth) affecting the diverse water users of this basin in terms of water shortage? What are the key drivers of this shortage for each user? Methods and results from this work are used to address additional questions on the ability of adaptation to modulate the effects of these uncertain drivers, and on their compounding effects across spatial scales and through sectoral interactions.

Hadjimichael, Antonia↗
DOE OSTI2026

Rapid Characterization Tools for Process Optimization: Cooperative Research and Development (Final Report)

In this project Prenexus Health, Inc. (Prenexus) and NLR will develop rapid characterization tools to improve the Prenexus production process. First, we will develop a tool to predict the overall performance of the Prenexus manufacturing process converting high-fiber sugarcane to xylo-oligosaccharides (XOS) (both the overall process yield and the composition of the XOS product) using rapid characterization of the incoming feedstock and knowledge of key process parameters. This tool will dramatically increase Prenexus’ understanding of the overall process. Second, we will develop a tool to predict the concentration of soluble oligomeric and monomeric xylose and organic acids in multiple process streams at-line in the Prenexus process, enabling real-time process control of the conversion process.

09 BIOMASS FUELS↗
DOE OSTI2021

Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

Prokaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle-like compartment in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryo-electron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Characterizing x-ray transmission through filters used in high energy density physics diagnostics

We report on the design and implementation of a new system used to characterize the energy-dependent x-ray transmission curve, Θ(E), through filters used in high-energy density physics diagnostics. Using an Amptek X-123-CdTe x-ray spectrometer together with a partially depleted silicon surface barrier detector, both the energy spectrum and total emission of an x-ray source have been accurately measured. By coupling these detectors with a custom PROTO-XRD x-ray source with interchangeable cathodes, accurate characterizations of Θ(E) for filters of varying materials and thicknesses have been obtained. The validity of the technique has been confirmed by accurately reproducing areal densities for high-purity filters with known x-ray transmission properties. In this paper, the experimental setup is described and the results of absorption calibrations performed on a variety of different filters are presented.

Pearcy, J.↗
NASA NTRS1971

Solid propellant selection and characterization

The techniques of propellant selection and characterization and the compromises that may be forced on the designer by the change of a propellant parameter are discussed. Rules for the selection of a general propellant type are presented. The characterization of various propellant properties is then approached parameter by parameter. Tailoring pitfalls and compromises are identified whenever possible, and appropriate cross references are used. Related design criteria monographs frequently are cited for a more detailed treatment of the topic under discussion. A list of source references is provided. These references, coupled with established texts in the field, should give the depth of knowledge necessary to the designer to aid in the selection of the most nearly optimum propellant for the design application.

Source record↗