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At least 1,243 records · Page 69

Optical Biosensor Platforms Display Varying Sensitivity for the Direct Detection of Influenza RNA

Detection methods that do not require nucleic acid amplification are advantageous for viral diagnostics due to their rapid results. These platforms could provide information for both accurate diagnoses and pandemic surveillance. Influenza virus is prone to pandemic-inducing genetic mutations, so there is a need to apply these detection platforms to influenza diagnostics. Here, we analyzed the Fast Evaluation of Viral Emerging Risks (FEVER) pipeline on ultrasensitive detection platforms, including a waveguide-based optical biosensor and a flow cytometry bead-based assay. The pipeline was also evaluated in silico for sequence coverage in comparison to the U.S. Centers for Disease Control and Prevention’s (CDC) influenza A and B diagnostic assays. The influenza FEVER probe design had a higher tolerance for mismatched bases than the CDC’s probes, and the FEVER probes altogether had a higher detection rate for influenza isolate sequences from GenBank. When formatted for use as molecular beacons, the FEVER probes detected influenza RNA as low as 50 nM on the waveguide-based optical biosensor and 1 nM on the flow cytometer. In addition to molecular beacons, which have an inherently high background signal we also developed an exonuclease selection method that could detect 500 pM of RNA. The combination of high-coverage probes developed using the FEVER pipeline coupled with ultrasensitive optical biosensors is a promising approach for future influenza diagnostic and biosurveillance applications.

59 BASIC BIOLOGICAL SCIENCES↗

Groundwater Depth Overrides Tree-Species Effects on the Structure of Soil Microbial Communities Involved in Nitrogen Cycling in Plantation Forests

Microbial communities found in soil ecosystems play important roles in decomposing organic materials and recycling nutrients. A clear understanding on how biotic and abiotic factors influence the microbial community and its functional role in ecosystems is fundamental to terrestrial biogeochemistry and plant production. The purpose of this study was to investigate microbial communities and functional genes involved in nitrogen cycling as a function of groundwater depth (deep and shallow), tree species (pine and eucalypt), and season (spring and fall). Soil fungal, bacterial, and archaeal communities were determined by length heterogeneity polymerase chain reaction (LH-PCR). Soil ammonia oxidation archaeal (AOA) amoA gene, ammonia oxidation bacterial (AOB) amoA gene, nitrite oxidoreductase nrxA gene, and denitrifying bacterial narG, nirK, nirS, and nosZ genes were further studied using PCR and denaturing gradient gel electrophoresis (DGGE). Soil fungal and bacterial communities remained similar between tree species and groundwater depths, respectively, regardless of season. Soil archaeal communities remained similar between tree species but differed between groundwater depths in the spring only. Archaeal amoA for nitrification and bacterial nirK and nosZ genes for denitrification were detected in DGGE, whereas bacterial amoA and nrxA for nitrification and bacterial narG and nirS genes for denitrification were undetectable. The detected nitrification and denitrification communities varied significantly with groundwater depth. There was no significant difference of nitrifying archaeal amoA or denitrifying nirK communities between different tree species regardless of season. The seasonal difference in microbial communities and functional genes involved in nitrogen cycling suggests microorganisms exhibit seasonal dynamics that likely impact relative rates of nitrification and denitrification.

54 ENVIRONMENTAL SCIENCES↗

Advancing Molecular Weight Determination of Lignin by Multi-Angle Light Scattering

Due to the complexity and recalcitrance of lignin, its chemical characterization is a key factor preventing the valorization of this abundant material. Multi-angle light scattering (MALS) is becoming a sought-after technique for absolute molecular weight (MW) determination of polymers and proteins. Lignin is a suitable candidate for MW determination via MALS, yet further investigation is required to confirm its absolute MW values and molecular size. Studies aiming to break down lignin into a variety of renewable products will benefit greatly from a simple and reliable determination method like MALS. Recent pioneering studies, discussed in this review, addressed several key challenges in lignin’s MW characterization. Nevertheless, some lignin-specific issues still need to be considered for in-depth characterization. This study explores how MALS instrumentation manages the complexities of determining lignin’s MW, e.g., with simultaneous fractionation and fluorescence interference mitigation. Additionally, we rationalize the importance of a more detailed light scattering analysis for lignin characterization, including aspects like the second virial coefficient and radius of gyration.

differential refractive index increment↗

A Study of the Radiation Tolerance of CVD Diamond to 70 MeV Protons, Fast Neutrons and 200 MeV Pions

We measured the radiation tolerance of commercially available diamonds grown by the Chemical Vapor Deposition process by measuring the charge created by a 120 GeV hadron beam in a 50 μm pitch strip detector fabricated on each diamond sample before and after irradiation. We irradiated one group of samples with 70 MeV protons, a second group of samples with fast reactor neutrons (defined as energy greater than 0.1 MeV), and a third group of samples with 200 MeV pions, in steps, to (8.8±0.9) × 1015 protons/cm2, (1.43±0.14) × 1016 neutrons/cm2, and (6.5±1.4) × 1014 pions/cm2, respectively. By observing the charge induced due to the separation of electron–hole pairs created by the passage of the hadron beam through each sample, on an event-by-event basis, as a function of irradiation fluence, we conclude all datasets can be described by a first-order damage equation and independently calculate the damage constant for 70 MeV protons, fast reactor neutrons, and 200 MeV pions. We find the damage constant for diamond irradiated with 70 MeV protons to be 1.62±0.07(stat)±0.16(syst)× 10−18 cm2/(p μm), the damage constant for diamond irradiated with fast reactor neutrons to be 2.65±0.13(stat)±0.18(syst)× 10−18 cm2/(n μm), and the damage constant for diamond irradiated with 200 MeV pions to be 2.0±0.2(stat)±0.5(syst)× 10−18 cm2/(π μm). The damage constants from this measurement were analyzed together with our previously published 24 GeV proton irradiation and 800 MeV proton irradiation damage constant data to derive the first comprehensive set of relative damage constants for Chemical Vapor Deposition diamond. We find 70 MeV protons are 2.60 ± 0.29 times more damaging than 24 GeV protons, fast reactor neutrons are 4.3 ± 0.4 times more damaging than 24 GeV protons, and 200 MeV pions are 3.2 ± 0.8 more damaging than 24 GeV protons. We also observe the measured data can be described by a universal damage curve for all proton, neutron, and pion irradiations we performed of Chemical Vapor Deposition diamond. Finally, we confirm the spatial uniformity of the collected charge increases with fluence for polycrystalline Chemical Vapor Deposition diamond, and this effect can also be described by a universal curve.

36 MATERIALS SCIENCE↗

Cradle-to-Grave Lifecycle Analysis of Greenhouse Gas Emissions of Light-Duty Passenger Vehicles in China: Towards a Carbon-Neutral Future

Vehicle electrification is considered a pathway for on-road transportation decarbonization in China. Different from the conventional gasoline vehicles whose emissions are mainly released from vehicle tailpipes, emissions of battery electric vehicles (BEVs) are from the upstream processes of electricity generation and vehicle manufacturing, thus a comprehensive lifecycle analysis and comparison of BEVs with gasoline vehicles is required to quantify the emission mitigation benefit of vehicle electrification and determine the path to a carbon-neutral future. In the study, we compare the cradle-to-grave (C2G) lifecycle greenhouse gas emissions of gasoline and electric vehicles in China and analyze the greenhouse gas emission reduction of vehicle electrification in different provinces. Results show that under the current technologies, the national average C2G GHG emissions for battery electric vehicles (BEVs) of 100 miles (i.e., 160 km) and 300 miles (i.e., 480 km) all-electric range (AER) are 231 and 279 g CO2eq/km, respectively, 22% and 5% lower than those for gasoline internal combustion engine vehicles (ICEVs). Improving vehicle fuel efficiency by hybridizing gasoline ICEVs can effectively reduce C2G emissions to 212 g CO2eq/km. At the provincial level, C2G GHG emissions of BEVs vary according to the provincial electricity mix. In eight provinces, C2G GHG emissions of BEVs with 300 miles AER (BEV300s) are higher than those of gasoline ICEVs due to the GHG-intensive coal-based electricity mix. In the future scenario, with low carbon fuels (such as high-level bioethanol blending gasoline) and electricity decarbonization, the national average C2G emissions of hybrid electric vehicles (HEVs) and BEV300s can be reduced to 55 and 73 g CO2eq/km, respectively. Further decrease of C2G GHG emissions relies on reducing vehicle-cycle emissions from material processing and vehicle component manufacturing.

33 ADVANCED PROPULSION SYSTEMS↗

TOI-503: The First Known Brown-dwarf Am-star Binary from the TESS Mission

We report the discovery of an intermediate-mass transiting brown dwarf (BD), TOI-503b, from the TESS mission. TOI-503b is the first BD discovered by TESS, and it has circular orbit around a metallic-line A-type star with a period of P = 3.6772 ± 0.0001 days. The light curve from TESS indicates that TOI-503b transits its host star in a grazing manner, which limits the precision with which we measure the BD’s radius (R{sub b}=1.34{sub −0.15}{sup +0.26}R{sub J}). We obtained high-resolution spectroscopic observations with the FIES, Ondřejov, PARAS, Tautenburg, and TRES spectrographs, and measured the mass of TOI-503b to be M {sub b} = 53.7 ± 1.2 M{sub J}. The host star has a mass of M {sub ⋆} = 1.80 ± 0.06 M {sub ⊙}, a radius of R {sub ⋆} = 1.70 ± 0.05R {sub ⊙}, an effective temperature of T {sub eff} = 7650 ± 160 K, and a relatively high metallicity of 0.61 ± 0.07 dex. We used stellar isochrones to derive the age of the system to be ∼180 Myr, which places its age between that of RIK 72b (a ∼10 Myr old BD in the Upper Scorpius stellar association) and AD 3116b (a ∼600 Myr old BD in the Praesepe cluster). Given the difficulty in measuring the tidal interactions between BDs and their host stars, we cannot precisely say whether this BD formed in situ or has had its orbit circularized by its host star over the relatively short age of the system. Instead, we offer an examination of plausible values for the tidal quality factor for the star and BD. TOI-503b joins a growing number of known short-period, intermediate-mass BDs orbiting main-sequence stars, and is the second such BD known to transit an A star, after HATS-70b. With the growth in the population in this regime, the driest region in the BD desert (35--55M{sub J}sin i) is reforesting.

79 ASTRONOMY AND ASTROPHYSICS↗

LHS 1815b: The First Thick-disk Planet Detected by TESS

We report the first discovery of a thick-disk planet, LHS 1815b (TOI-704b, TIC 260004324), detected in the Transiting Exoplanet Survey Satellite (TESS) survey. LHS 1815b transits a bright (V = 12.19 mag, K = 7.99 mag) and quiet M dwarf located 29.87 ± 0.02 pc away with a mass of 0.502 ± 0.015 M {sub ⊙} and a radius of 0.501 ± 0.030 R {sub ⊙}. We validate the planet by combining space- and ground-based photometry, spectroscopy, and imaging. The planet has a radius of 1.088 ± 0.064 R {sub ⊕} with a 3σ mass upper limit of 8.7 M {sub ⊕}. We analyze the galactic kinematics and orbit of the host star LHS 1815 and find that it has a large probability (P {sub thick}/P {sub thin} = 6482) to be in the thick disk with a much higher expected maximal height (Z {sub max} = 1.8 kpc) above the Galactic plane compared with other TESS planet host stars. Future studies of the interior structure and atmospheric properties of planets in such systems using, for example, the upcoming James Webb Space Telescope, can investigate the differences in formation efficiency and evolution for planetary systems between different Galactic components (thick disks, thin disks, and halo).

79 ASTRONOMY AND ASTROPHYSICS↗

Effective Opacity of the Intergalactic Medium from Galaxy Spectra Analysis

We measure the effective opacity (τ{sub eff}) of the intergalactic medium from the composite spectra of 281 Lyman-break galaxies in the redshift range 2 ≲ z ≲ 3. Our spectra are taken from the COSMOS Lyα Mapping And Tomographic Observations survey derived from the Low Resolution Imaging Spectrometer on the W.M. Keck I telescope. We generate composite spectra in two redshift intervals and fit them with spectral energy distribution (SED) models composed of simple stellar populations. Extrapolating these SED models into the Lyα forest, we measure the effective Lyα opacity (τ{sub eff}) in the 2.02 ≤ z ≤ 2.44 range. At z = 2.22, we estimate τ{sub eff} =0.159±0.001 from a power-law fit to the data. These measurements are consistent with estimates from quasar analyses at z < 2.5 indicating that the systematic errors associated with normalizing quasar continua are not substantial. We provide a Gaussian processes model of our results and previous τ{sub eff} measurements that describes the steep redshift evolution in τ{sub eff} from z = 1.5–4.

79 ASTRONOMY AND ASTROPHYSICS↗

TOI-2076 and TOI-1807: Two Young, Comoving Planetary Systems within 50 pc Identified by TESS that are Ideal Candidates for Further Follow Up

We report the discovery of two planetary systems around comoving stars: TOI-2076 (TIC 27491137) and TOI-1807 (TIC 180695581). TOI-2076 is a nearby (41.9 pc) multiplanetary system orbiting a young (204 ± 50 Myr), bright (K = 7.115 in TIC v8.1) start. TOI-1807 hosts a single transiting planet and is similarly nearby (42.58 pc), similarly young (180 ± 40 Myr ), and bright. Both targets exhibit significant, periodic variability due to starspots, characteristic of their young ages. Using photometric data collected by TESS we identify three transiting planets around TOI-2076 with radii of R {sub b} = 3.3 ± 0.04 R {sub ⊕}, R {sub c} = 4.4 ± 0.05 R {sub ⊕}, and R {sub d} = 4.1 ± 0.07 R {sub ⊕}. Planet TOI-2076b has a period of P {sub b} = 10.356 days. For both TOI-2076c and d, TESS observed only two transits, separated by a 2 yr interval in which no data were collected, preventing a unique period determination. A range of long periods (<17 days) are consistent with the data. We identify a short-period planet around TOI-1807 with a radius of R {sub b} = 1.8 ± 0.04 R {sub ⊕} and a period of P {sub b} = 0.549 days. Their close proximity, and bright, cool host stars, and young ages make these planets excellent candidates for follow up. TOI-1807b is one of the best-known small (R < 2 R{sub ⊕}) planets for characterization via eclipse spectroscopy and phase curves with JWST. TOI-1807b is the youngest ultra-short-period planet discovered to date, providing valuable constraints on formation timescales of short-period planets. Given the rarity of young planets, particularly in multiple-planet systems, these planets present an unprecedented opportunity to study and compare exoplanet formation, and young planet atmospheres, at a crucial transition age for formation theory.

47 OTHER INSTRUMENTATION↗

TARDIS. II. Synergistic Density Reconstruction from Lyα Forest and Spectroscopic Galaxy Surveys with Applications to Protoclusters and the Cosmic Web

Looking at this work, we expand upon the Tomographic Absorption Reconstruction and Density Inference Scheme (TARDIS) in order to include multiple tracers while reconstructing matter density fields at Cosmic Noon (z ~ 2–3). In particular, we jointly reconstruct the underlying density field from simulated Lyα forest observations at z ~ 2.5 and an overlapping galaxy survey. We find that these data are synergistic, with the Lyα forest providing reconstruction of low-density regions and galaxy surveys tracing the density peaks. We find a more accurate power spectra reconstruction going to higher scales when fitting these two data sets simultaneously than when using either one individually. When applied to cosmic web analysis, we find that performing the joint analysis is equivalent to an Lγα survey with significantly increased sight-line spacing. Because we reconstruct the velocity field and matter field jointly, we demonstrate the ability to evolve the mock observed volume further to z = 0, allowing us to create a rigorous definition of a “protocluster” as regions that will evolve into clusters. We apply our reconstructions to study protocluster structure and evolution, finding for realistic survey parameters that we can provide accurate mass estimates of the z ≈ 2 structures and their z = 0 fate.

79 ASTRONOMY AND ASTROPHYSICS↗

Magnetic Energy Conversion in Magnetohydrodynamics: Curvature Relaxation and Perpendicular Expansion of Magnetic Fields

Abstract The mechanisms and pathways of magnetic energy conversion are an important subject for many laboratory, space, and astrophysical systems. Here, we present a perspective on magnetic energy conversion in magnetohydrodynamics through magnetic field curvature relaxation (CR) and perpendicular expansion (PE) due to magnetic pressure gradients, and quantify their relative importance in two representative cases, namely 3D magnetic reconnection and 3D kink-driven instability in an astrophysical jet. We find that the CR and PE processes have different temporal and spatial evolutions in these systems. The relative importance of the two processes tends to reverse as the system enters the nonlinear stage from the instability growth stage. Overall, the two processes make comparable contributions to magnetic energy conversion, with the PE process somewhat stronger than the CR process. We further explore how these energy conversion terms can be related to particle energization in these systems.

79 ASTRONOMY AND ASTROPHYSICS↗

On the Existence of Fast Modes in Compressible Magnetohydrodynamic Turbulence

Abstract We study the existence and properties of fast magnetosonic modes in 3D compressible MHD turbulence by carrying out a number of simulations with compressible and incompressible driving conditions. We use two approaches to determine the presence of fast modes: mode decomposition based on spatial variations only and spatio-temporal 4D fast Fourier transform (4D FFT) analysis of all fluctuations. The latter method enables us to quantify fluctuations that satisfy the dispersion relation of fast modes with finite frequency. Overall, we find that the fraction of fast modes identified via the spatio-temporal 4D FFT approach in total fluctuation power is either tiny with nearly incompressible driving or ∼2% with highly compressible driving. We discuss the implications of our results for understanding the compressible fluctuations in space and astrophysical plasmas.

79 ASTRONOMY AND ASTROPHYSICS↗

Nature and Scalings of Density Fluctuations of Compressible Magnetohydrodynamic Turbulence with Applications to the Solar Wind

Abstract The solar wind is a magnetized and turbulent plasma. Its turbulence is often dominated by Alfvénic fluctuations and often deemed as nearly incompressible far away from the Sun, as shown by in situ measurements near 1 au. However, for solar wind closer to the Sun, the plasma β decreases (often lower than unity) while the turbulent Mach number M t increases (can approach unity, e.g., transonic fluctuations). These conditions could produce significantly more compressible effects, characterized by enhanced density fluctuations, as seen by several space missions. In this paper, a series of 3D MHD simulations of turbulence are carried out to understand the properties of compressible turbulence, particularly the generation of density fluctuations. We find that, over a broad range of parameter space in plasma β , cross helicity, and polytropic index, the turbulent density fluctuations scale linearly as a function of M t , with the scaling coefficients showing weak dependence on parameters. Furthermore, through detailed spatiotemporal analysis, we show that the density fluctuations are dominated by low-frequency nonlinear structures, rather than compressible MHD eigenwaves. These results could be important for understanding how compressible turbulence contributes to solar wind heating near the Sun.

79 ASTRONOMY AND ASTROPHYSICS↗

Anisotropic Energy Transfer and Conversion in Magnetized Compressible Turbulence

We present a spatial filtering (or coarse-graining) analysis on 3D magnetized magnetohydrodynamic (MHD) turbulence simulations. The filtered compressible MHD formulae show transfer of kinetic and magnetic energies from large to small scales, as well as energy conversion between kinetic, magnetic, and thermal energies. The anisotropic filtering enables separate analyses of the energy flows perpendicular and parallel to the global mean magnetic field. Anisotropy in energy cascade is demonstrated by the larger perpendicular energy cascade rate and also the larger perpendicular wavenumbers associated with the peak energy transfer rate. We also find that the “inertial range” along the parallel (perpendicular) direction in the anisotropic energy cascade formulation is no longer strictly dissipation-free, because it includes the dissipation in the perpendicular (parallel) direction. A change in the driving force (kinetic only versus kinetic and magnetic) affects the energy conversion between kinetic and magnetic energies. While the compressibility of the driving force changes the partition of different channels of energy transfer and conversion, and also increases the total energy transfer rate, the global energy flow remains unaffected by compressibility qualitatively. Our analysis can be applied to multispacecraft observations of turbulence in the solar wind or a planetary magnetosphere.

79 ASTRONOMY AND ASTROPHYSICS↗

Alignments between Galaxies and the Cosmic Web at z ~ 1–2 in the IllustrisTNG Simulations

Galaxy formation theories predict that galaxy shapes and angular momenta have nonrandom alignments with the cosmic web. This leads to so-called intrinsic alignment between pairs of galaxies, which is important to quantify as a nuisance parameter for weak lensing. We study galaxy–cosmic web alignment in the IllustrisTNG suite of hydrodynamical simulations at redshifts 1 and 2, finding that alignment trends are consistent with previous studies. However, we find that the magnitude of the spin alignment signal is ~2.4× weaker than seen in previous studies of the Horizon-AGN simulation, suggesting that this signal may have a significant dependence on subgrid physics. Based on IllustrisTNG, we then construct mock observational spectroscopic surveys that can probe shape–cosmic web alignment at z ~ 1–2, modeled on the low-z galaxy redshift and IGM tomography surveys on the upcoming Subaru Prime Focus Spectrograph Galaxy Evolution (PFS GE) survey. However, even over box sizes of L = 205 h -1 Mpc, we find that global anisotropies induce a sample variance in the 2D projected alignment signal that depend on the projected direction; this induces significant errors in the observed alignment. We predict a 5.3σ detection of IllustrisTNG's shape alignment signal at z ~ 1 from Subaru PFS GE, although a detection would be challenging at z ~ 2. However, a rough rescaling of the relative alignment signal strengths between the TNG and Horizon-AGN simulations suggests that PFS GE should be able to more easily constrain the latter's stronger signal.

79 ASTRONOMY AND ASTROPHYSICS↗

Aligning Standards Communities for Omics Biodiversity Data: Sustainable Darwin Core-MIxS Interoperability

The standardization of data, encompassing both primary and contextual information (metadata), plays a pivotal role in facilitating data (re-)use, integration, and knowledge generation. However, the biodiversity and omics communities, converging on omics biodiversity data, have historically developed and adopted their own distinct standards, hindering effective (meta)data integration and collaboration. In response to this challenge, the Task Group (TG) for Sustainable DwC-MIxS Interoperability was established. Convening experts from the Biodiversity Information Standards (TDWG) and the Genomic Standards Consortium (GSC) alongside external stakeholders, the TG aimed to promote sustainable interoperability between the Minimum Information about any (x) Sequence (MIxS) and Darwin Core (DwC) specifications. To achieve this goal, the TG utilized the Simple Standard for Sharing Ontology Mappings (SSSOM) to create a comprehensive mapping of DwC keys to MIxS keys. This mapping, combined with the development of the MIxS-DwC extension, enables the incorporation of MIxS core terms into DwC-compliant metadata records, facilitating seamless data exchange between MIxS and DwC user communities. Through the implementation of this translation layer, data produced in either MIxS- or DwC-compliant formats can now be efficiently brokered, breaking down silos and fostering closer collaboration between the biodiversity and omics communities. To ensure its sustainability and lasting impact, TDWG and GSC have both signed a Memorandum of Understanding (MoU) on creating a continuous model to synchronize their standards. These achievements mark a significant step forward in enhancing data sharing and utilization across domains, thereby unlocking new opportunities for scientific discovery and advancement.

59 BASIC BIOLOGICAL SCIENCES↗