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At least 1,225 records · Page 68

Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals

The crystallization of recombinant proteins in living cells is an exciting new approach in structural biology. Recent success has highlighted the need for fast and efficient diffraction data collection, optimally directly exposing intact crystal-containing cells to the X-ray beam, thus protecting the in cellulo crystals from environmental challenges. Serial femtosecond crystallography (SFX) at free-electron lasers (XFELs) allows the collection of detectable diffraction even from tiny protein crystals, but requires very fast sample exchange to utilize each XFEL pulse. Here, an efficient approach is presented for high-resolution structure elucidation using serial femtosecond in cellulo diffraction of micometre-sized crystals of the protein HEX-1 from the fungus Neurospora crassa on a fixed target. Employing the fast and highly accurate Roadrunner II translation-stage system allowed efficient raster scanning of the pores of micro-patterned, single-crystalline silicon chips loaded with living, crystal-containing insect cells. Compared with liquid-jet and LCP injection systems, the increased hit rates of up to 30% and reduced background scattering enabled elucidation of the HEX-1 structure. Using diffraction data from only a single chip collected within 12 min at the Linac Coherent Light Source, a 1.8 Å resolution structure was obtained with significantly reduced sample consumption compared with previous SFX experiments using liquid-jet injection. This HEX-1 structure is almost superimposable with that previously determined using synchrotron radiation from single HEX-1 crystals grown by sitting-drop vapour diffusion, validating the approach. This study demonstrates that fixed-target SFX using micro-patterned silicon chips is ideally suited for efficient in cellulo diffraction data collection using living, crystal-containing cells, and offers huge potential for the straightforward structure elucidation of proteins that form intracellular crystals at both XFELs and synchrotron sources.

Lahey-Rudolph, J. Mia (ORCID:0000000152797267)↗

Air quality impact of the Northern California Camp Fire of November 2018

The Northern California Camp Fire that took place in November 2018 was one of the most damaging environmental events in California history. Here, we analyze ground-based station observations of airborne particulate matter that has a diameter <2.5 µm (PM 2.5 ) across Northern California and conduct numerical simulations of the Camp Fire using the Weather Research and Forecasting model online coupled with chemistry (WRF-Chem). Simulations are evaluated against ground-based observations of PM 2.5 , black carbon, and meteorology, as well as satellite measurements, such as Tropospheric Monitoring Instrument (TROPOMI) aerosol layer height and aerosol index. The Camp Fire led to an increase in Bay Area PM 2.5 to over 50 µg m –3 for nearly 2 weeks, with localized peaks exceeding 300 µg m –3 . Using the Visible Infrared Imaging Radiometer Suite (VIIRS) high-resolution fire detection products, the simulations reproduce the magnitude and evolution of surface PM 2.5 concentrations, especially downwind of the wildfire. The overall spatial patterns of simulated aerosol plumes and their heights are comparable with the latest satellite products from TROPOMI. WRF-Chem sensitivity simulations are carried out to analyze uncertainties that arise from fire emissions, meteorological conditions, feedback of aerosol radiative effects on meteorology, and various physical parameterizations, including the planetary boundary layer model and the plume rise model. Downwind PM 2.5 concentrations are sensitive to both flaming and smoldering emissions over the fire, so the uncertainty in the satellite-derived fire emission products can directly affect the air pollution simulations downwind. Our analysis also shows the importance of land surface and boundary layer parameterization in the fire simulation, which can result in large variations in magnitude and trend of surface PM 2.5 . Inclusion of aerosol radiative feedback moderately improves PM 2.5 simulations, especially over the most polluted days. Results of this study can assist in the development of data assimilation systems as well as air quality forecasting of health exposures and economic impact studies.

54 ENVIRONMENTAL SCIENCES↗

Case Study of Using High Performance Commercial Processors in Space

The purpose of the Space Shuttle Cockpit Avionics Upgrade project (1999 2004) was to reduce crew workload and improve situational awareness. The upgrade was to augment the Shuttle avionics system with new hardware and software. A major success of this project was the validation of the hardware architecture and software design. This was significant because the project incorporated new technology and approaches for the development of human rated space software. An early version of this system was tested at the Johnson Space Center for one month by teams of astronauts. The results were positive, but NASA eventually cancelled the project towards the end of the development cycle. The goal to reduce crew workload and improve situational awareness resulted in the need for high performance Central Processing Units (CPUs). The choice of CPU selected was the PowerPC family, which is a reduced instruction set computer (RISC) known for its high performance. However, the requirement for radiation tolerance resulted in the re-evaluation of the selected family member of the PowerPC line. Radiation testing revealed that the original selected processor (PowerPC 7400) was too soft to meet mission objectives and an effort was established to perform trade studies and performance testing to determine a feasible candidate. At that time, the PowerPC RAD750s were radiation tolerant, but did not meet the required performance needs of the project. Thus, the final solution was to select the PowerPC 7455. This processor did not have a radiation tolerant version, but had some ability to detect failures. However, its cache tags did not provide parity and thus the project incorporated a software strategy to detect radiation failures. The strategy was to incorporate dual paths for software generating commands to the legacy Space Shuttle avionics to prevent failures due to the softness of the upgraded avionics.

Ferguson, Roscoe C.↗

Plasma IMS Composition Measurements for Europa, Ganymede, and the Jovian Systems

NASA and ESA are now planning a reduced version of the joint Europa Jupiter System Mission (EJSM), potentially including a radically descoped Jupiter Europa Orbiter (JEO) but still with magnetometer and plasma instruments. Similar field and plasma instrumentation would also reside on ESA's Jupiter Ganymede Orbiter (JGO), which conceivably could carry out multiple flybys of Europa before entering orbit at Ganymede. We are developing the 3D Ion Mass Spectrometer (IMS) designed to measure both major and minor ion species within the high radiation environment of Jupiter s magnetosphere and the icy Galilean moons. The IMS covers the energy range from 10 eV to 30 keV, wide field-ofview (FOV) capability and 10-60 sec time resolution for major ions. This instrument has two main goals: 1) measure the plasma interaction between Europa and Jupiter s magnetosphere and 2) infer the global surface composition to trace elemental and significant isotopic levels; these goals are also applicable for in-situ measurements at Ganymede and Callisto, and remotely everywhere via the iogenic plasma for Io. The first goal supports the magnetometer (MAG) measurements, primarily directed at detection of Europa's sub-surface ocean, while the second goal gives information about transfer of material between the Galilean moons, e.g. mainly from Io to the other moons, and further allows detection of oceanic materials emergent to the moon surfaces from subsurface layers putatively including salt water oceans. Outgassed exospheric materials are probed by the IMS by measuring pickup ions accelerated up to spacecraft altitudes of approximately 100-200 km in electric fields extending through the local magnetospheric environment and moon exosphere to the surface. Our 3D hybrid kinetic model of the moon-magnetosphere interaction is used to construct a global model of electric and magnetic fields for tracing of pickup ion trajectories back to the sources at approximate surface resolution of 100 km. We show that Europa's exospheric ionosphere is dominated by pickup ions with energies of 100-1000 eV. We also expect field aligned polar ion outflows driven by ionospheric electrons via the polarization electric field at Europa; the IMS will observe such outflows and thus sample the ionosphere below spacecraft orbit altitude approximately 100 km. Based on previous Ganymede studies, we also comment on IMS applications to a Ganymede orbiter. The IMS and the Europa interaction model are respectively being developed with support from NASA's Astrobiology Instrument Development (ASTID) and Outer Planets Research (OPR) programs.

Sittler, E.↗

A Search for Technosignatures Around 11,680 Stars with the Green Bank Telescope at 1.15–1.73 GHz

We conducted a search for narrowband radio signals over four observing sessions in 2020–2023 with the $L$-band receiver (1.15–1.73 GHz) of the 100 m diameter Green Bank Telescope. We pointed the telescope in the directions of 62 TESS Objects of Interest, capturing radio emissions from a total of ~11,680 stars and planetary systems in the ~9' beam of the telescope. All detections were either automatically rejected or visually inspected and confirmed to be of anthropogenic nature. We also quantified the end-to-end efficiency of radio SETI pipelines with a signal injection and recovery analysis. The UCLA SETI pipeline recovers 94.0% of the injected signals over the usable frequency range of the receiver and 98.7% of the injections when regions of dense radio frequency interference are excluded. In another pipeline that uses incoherent sums of 51 consecutive spectra, the recovery rate is ~15 times smaller at ~6%. The pipeline efficiency affects calculations of transmitter prevalence and SETI search volume. Accordingly, we developed an improved Drake figure of merit and a formalism to place upper limits on transmitter prevalence that take the pipeline efficiency and transmitter duty cycle into account. Based on our observations, we can state at the 95% confidence level that fewer than 6.6% of stars within 100 pc host a transmitter that is continuously transmitting a narrowband signal with an equivalent isotropic radiated power (EIRP) > 10 13 W. For stars within 20,000 ly, the fraction of stars with detectable transmitters (EIRP > 5 × 10 16 W) is at most 3 × 10 -4 . Finally, we showed that the UCLA SETI pipeline natively detects the signals detected with AI techniques by Ma et al.

79 ASTRONOMY AND ASTROPHYSICS↗

Saturnian trapped radiation and its absorption by satellites and rings - The first results from Pioneer 11

Preliminary results from Pioneer 11 concerning the acceleration and trapping of charged particles in the magnetic field of Saturn are reported. The identification and measurement of the intensities and spectra of charged particle species was performed by an experiment including four charged particle sensor systems, within 20 Saturn radii of the planet. Increases in the intensity of 0.5- to 1.8-MeV protons within 15 Saturn radii indicate the trapping and acceleration of particles in the dipole field region, while a decrease in proton intensity between seven and four Saturn radii is attributed to absorption by Dione and Enceladus and possibly ring material as well. Proton and electron intensity distributions are found to be axially symmetric within four Saturn radii, indicating a centered dipole aligned with the planetary rotation axis. Trapped radiation absorption at the orbit of Mimas is analyzed to obtain an upper limit of 4 x 10 to the -8th Saturn radii-squared/sec to the inward diffusion coefficient; an absorption-like feature observed at L = 2.5 is attributed to a previously unidentified satellite of diameter less than 200 km and semimajor axis 2.51 Saturn radii. Radiation absorption by the newly discovered F ring was also observed, however beneath the A, B and C rings a low flux of high-energy electrons was detected.

Simpson, J. A.↗

Heavy ion action on single cells: Cellular inactivation capability of single accelerated heavy ions

Heavy ions (HZE-particles) constitute an important part of radiation in space. Although their number is small the high amount of energy transferred by individual particles may cause severe biological effects. Their investigation requires special techniques which were tested by experiments performed at the UNILAC at the GSI (Darmstadt). Diploid yeast was used which is a suitable eucaryotic test system because of its resistance to extreme conditions like dryness and vacuum. Cells were placed on nuclear track detector foils and exposed to ions of different atomic number and energy. To assess the action of one single ion on an individual cell, track parameters and the respective colony forming abilities (CFA) were determined with the help of computer aided image analysis. There is mounting evidence that not only the amount of energy deposited along the particle path, commonly given by the LET, is of importance but also the spatial problem of energy deposition at a submicroscopical scale. It is virtually impossible to investigate track structure effects in detail with whole cell populations and (globally applied) high particle fluences. It is, therefore, necessary to detect the action of simple ions in individual cells. The results show that the biological action depends on atomic number and specific energy of the impinging ions, which can be compared with model calculations of recent track structure models.

Kost, M.↗

The Single Event Effect Characteristics of the 486-DX4 Microprocessor

This research describes the development of an experimental radiation testing environment to investigate the single event effect (SEE) susceptibility of the 486-DX4 microprocessor. SEE effects are caused by radiation particles that disrupt the logic state of an operating semiconductor, and include single event upsets (SEU) and single event latchup (SEL). The relevance of this work can be applied directly to digital devices that are used in spaceflight computer systems. The 486-DX4 is a powerful commercial microprocessor that is currently under consideration for use in several spaceflight systems. As part of its selection process, it must be rigorously tested to determine its overall reliability in the space environment, including its radiation susceptibility. The goal of this research is to experimentally test and characterize the single event effects of the 486-DX4 microprocessor using a cyclotron facility as the fault-injection source. The test philosophy is to focus on the "operational susceptibility," by executing real software and monitoring for errors while the device is under irradiation. This research encompasses both experimental and analytical techniques, and yields a characterization of the 486-DX4's behavior for different operating modes. Additionally, the test methodology can accommodate a wide range of digital devices, such as microprocessors, microcontrollers, ASICS, and memory modules, for future testing. The goals were achieved by testing with three heavy-ion species to provide different linear energy transfer rates, and a total of six microprocessor parts were tested from two different vendors. A consistent set of error modes were identified that indicate the manner in which the errors were detected in the processor. The upset cross-section curves were calculated for each error mode, and the SEU threshold and saturation levels were identified for each processor. Results show a distinct difference in the upset rate for different configurations of the on-chip cache, as well as proving that one vendor is superior to the other in terms of latchup susceptibility. Results from this testing were also used to provide a mean-time-between-failure estimate of the 486-DX4 operating in the radiation environment for the International Space Station.

Kouba, Coy↗

Plasma IMS Composition Measurements for Europa, Ganymede, and the Jovian System

NASA and ESA are now planning a reduced version of the joint Europa Jupiter System Mission (EJSM), potentially including a radically descoped Jupiter Europa Orbiter (JEO) but still with magnetometer and plasma instruments. Similar field and plasma instrumentation would also reside on ESA's Jupiter Ganymede Orbiter (JGO), which conceivably could carry out multiple flybys of Europa before entering orbit at Ganymede. We are developing the 3D Ion Mass Spectrometer (IMS) designed to measure both major and minor ion species within the high radiation environment of Jupiter's magnetosphere and the icy Galilean moons. The IMS covers the energy range from 10 eV to 30 keY, wide field-of-view (FOV) capability and 10-60 sec time resolution for major ions. This instrument has two main goals: 1) measure the plasma interaction between Europa and Jupiter's magnetosphere and 2) infer the global surface composition to trace elemental and significant isotopic levels; these goals are also applicable for in-situ measurements at Ganymede and Callisto, and remotely everywhere via the iogenic plasma for 10. The first goal supports the magnetometer (MAG) measurements, primarily directed at detection of Europa's sub-surface ocean, while the second goal gives information about transfer of material between the Galilean moons, e.g. mainly from 10 to the other moons, and further allows detection of oceanic materials emergent to the moon surfaces from subsurface layers putatively including salt water oceans. Outgassed exospheric materials are probed by the IMS by measuring pickup ions accelerated up to spacecraft altitudes of approximately 100-200 km in electric fields extending through the local magnetospheric environment and moon exosphere to the surface. Our 3D hybrid kinetic model of the moon-magnetosphere interaction is used to construct a global model of electric and magnetic fields for tracing of pickup ion trajectories back to the sources at approximate surface resolution of 100 km. We show that Europa's exospheric ionosphere is dominated by pickup ions with energies of 100-1000 eV. We also expect field aligned polar ion outflows driven by ionospheric electrons via the polarization electric field at Europa; the IMS will observe such outflows and thus sample the ionosphere below spacecraft orbit altitude approximately 100 km. Based on previous Ganymede studies, we also comment on IMS applications to a Ganymede orbiter. The IMS and the Europa interaction model are respectively being developed with support from NASA's Astrobiology Instrument Development (ASTID) and Outer Planets Research (OPR) programs.

Sittler, E. C., Jr.↗

Potential Nighttime Contamination of CERES Clear-sky Field of View by Optically Thin Cirrus during the CRYSTAL-FACE Campaign

We investigate the outgoing broadband longwave (LW, 5 to approx. 200 microns) and window (WIN, 8 to approx. 12 microns) channel radiances at the top of atmosphere (TOA) under clear-sky conditions, using data acquired by the Cloud and the Earth s Radiant Energy System (CERES) and Moderate-Resolution Imaging Spectroradiometer (MODIS) instruments onboard the NASA Terra satellite platform. In this study, detailed analyses are performed on the CERES Single Scanner Footprint TOA/Surface Fluxes and Clouds product to understand the radiative effect of thin cirrus. The data are acquired over the Florida area during the Cirrus Regional Study of Tropical Anvils and Cirrus Layers Florida Area Cirrus Experiment (CRYSTAL-FACE) field program. Of particular interest is the anisotropy associated with the radiation field. Measured CERES broadband radiances are compared to those obtained from rigorous radiative transfer simulations. Analysis of results from this comparison indicates that the simulated radiances tend to be larger than their measured counterparts, with differences ranging from 2.1% to 8.3% for the LW band and from 1.7% to 10.6% for the WIN band. The averaged difference in radiance is approximately 4% for both the LW and WIN channels. A potential cause for the differences could be the presence of thin cirrus (i.e., optically thin ice clouds with visible optical thicknesses smaller than approximately 0.3). The detection and quantitative analysis of these thin cirrus clouds are challenging even with sophisticated multispectral instruments. While large differences in radiance between the CERES observations and the theoretical calculations are found, the corresponding difference in the anisotropic factors is very small (0.2%). Furthermore, sensitivity studies show that the influence due to a 1 K bias of the surface temperature on the errors of the LW and WIN channel radiances is of the same order as that associated with a 2% bias of the surface emissivity. The LW and WIN errors associated with a 5% bias of water vapor amount in the lower atmosphere in conjunction with a 50% bias of water vapor amount in the upper atmosphere is similar to that of a 1 K bias of the vertical temperature profile. Even with the uncertainties considered for these various factors, the simulated LW and WIN radiances are still larger than the observed radiances if thin cirrus clouds are excluded.

Lee, Yong-Keun↗

Case Study of Using High Performance Commercial Processors in a Space Environment

The purpose of the Space Shuttle Cockpit Avionics Upgrade project was to reduce crew workload and improve situational awareness. The upgrade was to augment the Shuttle avionics system with new hardware and software. A major success of this project was the validation of the hardware architecture and software design. This was significant because the project incorporated new technology and approaches for the development of human rated space software. An early version of this system was tested at the Johnson Space Center for one month by teams of astronauts. The results were positive, but NASA eventually cancelled the project towards the end of the development cycle. The goal to reduce crew workload and improve situational awareness resulted in the need for high performance Central Processing Units (CPUs). The choice of CPU selected was the PowerPC family, which is a reduced instruction set computer (RISC) known for its high performance. However, the requirement for radiation tolerance resulted in the reevaluation of the selected family member of the PowerPC line. Radiation testing revealed that the original selected processor (PowerPC 7400) was too soft to meet mission objectives and an effort was established to perform trade studies and performance testing to determine a feasible candidate. At that time, the PowerPC RAD750s where radiation tolerant, but did not meet the required performance needs of the project. Thus, the final solution was to select the PowerPC 7455. This processor did not have a radiation tolerant version, but faired better than the 7400 in the ability to detect failures. However, its cache tags did not provide parity and thus the project incorporated a software strategy to detect radiation failures. The strategy was to incorporate dual paths for software generating commands to the legacy Space Shuttle avionics to prevent failures due to the softness of the upgraded avionics.

Ferguson, Roscoe C.↗

High-Resolution Mapping of SO2 Using Airborne Observations from the GeoTASO Instrument During the KORUS-AQ Field Study: PCA-Based Vertical Column Retrievals

The Geostationary Trace gas and Aerosol Sensor Optimization (GeoTASO) instrument is an airborne hyperspectral spectrometer measuring backscattered solar radiation in the ultraviolet (290–400 nm) and visible (415–695 nm) wavelength regions. This paper presents high-resolution sulfur dioxide (SO2) maps over the Korean Peninsula, produced by SO2 retrievals from GeoTASO measurements during the Korea–United States Air Quality Field Study (KORUS-AQ) from May to June 2016. The highly sensitive GeoTASO instrument with a spatial resolution of ~250 m × 250 m can detect point emission sources of SO2 within its fields of view, even without merging multiple overlapping observations. To retrieve SO2 vertical columns from the GeoTASO measurements, we apply an algorithm based on principal component analysis (PCA), which is effective in suppressing noise and biases in SO2 retrievals. The retrievals successfully capture SO2 plumes and various point sources such as power plants, a petrochemical complex, and a steel mill, located in South Chungcheong Province, some of which are not detected by a ground-based in situ measurement network. Spatial distributions of SO2 from GeoTASO observations in source areas are consistent with those from the Stack Tele-Monitoring System reports and airborne in situ SO2 measurements. Comparisons of SO2 retrievals from GeoTASO and existing satellite sensors demonstrate the significance of high-resolution SO2 observations, by indicating that GeoTASO detects small SO2 emission sources that are not precisely resolved by single overpasses of satellites. To assess future geostationary SO2 observations having a higher spatial resolution, we upscale the GeoTASO SO2 retrievals to a spatial resolution of the Geostationary Environment Monitoring Spectrometer (GEMS). Since the upscaled GeoTASO retrievals also detect SO2 plumes clearly, we expect from GEMS to identify even small SO2 emission sources over Asia.

Chong, Heesung↗

Electronic spectroscopy and ionization potentials for YbOH and YbOCH 3

The polyatomic molecules YbOH and YbOCH 3 have been recognized as being of potential value for spectroscopic experiments that explore charge-parity and time reversal symmetry violation effects. These measurements require very high precision, which, in turn, will necessitate that the molecules be manipulated at ultra-cold temperatures. Both YbOH and YbOCH 3 have electronic transitions that appear suitable for laser cooling ($\tilde{A}$ 2 Π 1/2 -$\tilde{X}$ 2 Σ + and $\tilde{A}$2E 1/2 -$\tilde{X}$ 2 A 1 , respectively) but the currently available spectroscopic data is not sufficient to determine the extent to which population leaks may compromise the optical cooling processes. A further complication is that the quantum states of interest for these measurements will need to be selectively populated. The $\tilde{A}$-$\tilde{X}$ band systems of both YbOH and YbOCH 3 show evidence of vibronic perturbations, such that there are unassigned vibronic features at energies that are just above the origin bands. Here, in the present study we have recorded spectra for the $\tilde{A}$ 2 Π 1/2 -$\tilde{X}$ 2 Σ + transition of jet-cooled YbOD to facilitate the vibronic assignments. In addition, spectra for the $\tilde{B}$ 2 Σ + - $\tilde{X}$ 2 Σ + transition of YbOH were recorded, establishing the origin band at 20473.8 cm -1 . Previously, the reaction of Yb with CH 3 OH has been used to generate gas-phase YbOCH 3 . As this reaction also yields YbOH, there have been complications in spectroscopic studies of YbOCH 3 due to overlap of the $\tilde{A} -\tilde{X}$ band systems. To identify specific regions of overlap, resonantly enhanced two-photon ionization spectra were recorded using mass-resolved detection of the YbOH + and YbOCH 3 + ions. These data confirmed the overlap of vibronic bands near 17640 and 17680 cm -1 . Two-photon ionization spectroscopy also provided accurate ionization energies of IE(YbOH)=45788(10) and IE(YbOCH 3 )=45283(10) cm -1 . The IE for YbOH is relevant to problems encountered in previous attempts to determine the bond dissociation energy of YbOH + .

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Quality-Controlled Meteorological Data from the Flood Control District of Maricopa County (FCDMC) Network, Phoenix, Arizona (1987-2024)

This dataset contains 15- or 30-minute interval meteorological data from the Flood Control District of Maricopa County (FCDMC), Arizona, USA, covering eight key variables across multiple sensor stations between 1987 and 2024. Each variable is stored as a separate CSV file, containing time-series data that have undergone rigorous quality control (QC) procedures and, where appropriate, short-gap interpolation for consistency. The quality control (QC) pipeline consisted of four sequential tests: (1) a range test to ensure all values fall within physically realistic limits, (2) a step test to identify abrupt and implausible changes between consecutive records, (3) a proximity test that validates flagged values from step test using data from nearby stations and exceedance probability thresholds, and (4) a persistence test to detect and remove periods of unrealistically constant readings. These thresholds were calibrated to Arizona’s environmental conditions and sensor specifications. After QC, short gaps (≤2 hours) were linearly interpolated to ensure consistent temporal resolution, except for wind variables. Due to a major upgrade in FCDMC’s data transmission system, only ALERT-2 protocol data (2016–2024) for wind variables are included; earlier ALERT-1 data were excluded because of irregular sampling and high missing rates. This dataset supports regional climate and infrastructure resilience studies by providing standardized, high-resolution meteorological data for the greater Phoenix metropolitan area.

54 ENVIRONMENTAL SCIENCES↗

Direct Bayesian inference for fault severity assessment in Digital-Twin-Based fault diagnosis

For applications in condition-based maintenance of nuclear systems, the assessment of fault severity is crucial. In this work, we developed a framework that allows for direct inference of the probability distributions of possible faults in a system. Here, we employed a model-based approach with model residuals generated from analytical redundancy relations provided by physics-based models of the system components. From real-time sensor readings, the values of the model residuals can be calculated, and the posterior probability distributions of the faults can be computed directly using the methods of Bayesian networks. From the posterior distribution of each fault, one can estimate the fault probability based on a chosen threshold and assess the severity of the fault. By eliminating the discretization and simplifications in middle steps, this approach allows us to leverage the available computational resources to provide more accurate fault probability estimates and severity assessments.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Probing cosmic inflation with the LiteBIRD cosmic microwave background polarization survey

LiteBIRD, the Lite (Light) satellite for the study of B-mode polarization and Inflation from cosmic background Radiation Detection, is a space mission for primordial cosmology and fundamental physics. The Japan Aerospace Exploration Agency (JAXA) selected LiteBIRD in May 2019 as a strategic large-class (L-class) mission, with an expected launch in the late 2020s using JAXA’s H3 rocket. LiteBIRD is planned to orbit the Sun–Earth Lagrangian point L2, where it will map the cosmic microwave background polarization over the entire sky for three years, with three telescopes in 15 frequency bands between 34 and 448 GHz, to achieve an unprecedented total sensitivity of $2.2\, \mu$K-arcmin, with a typical angular resolution of 0.5° at 100 GHz. The primary scientific objective of LiteBIRD is to search for the signal from cosmic inflation, either making a discovery or ruling out well-motivated inflationary models. The measurements of LiteBIRD will also provide us with insight into the quantum nature of gravity and other new physics beyond the standard models of particle physics and cosmology. We provide an overview of the LiteBIRD project, including scientific objectives, mission and system requirements, operation concept, spacecraft and payload module design, expected scientific outcomes, potential design extensions, and synergies with other projects.

59 BASIC BIOLOGICAL SCIENCES↗

Lunar Lava Tubes - The Promise of New Orbital Data

The basaltic plains of the Moon contain lava channels on scales of tens of meters to hundreds of kilometers. Many of these channels are segmented, strongly suggesting that some portions include covered lava tubes. Lunar lava tubes are expected to provide unique environments below the harsh lunar surface, maintaining near-isothermal conditions and substantial shielding from solar and galactic radiation. A lava tube has often been suggested as natural shelter for a future human outpost. Previous searches for lunar lava tubes have been limited by a combination of image resolution and completeness of coverage. The five robotic Lunar Orbiter spacecraft combined to photograph essentially the entire lunar surface with a resolution of 60 m, and covered selected sites with resolutions as high as 2 m. The highest-resolution Apollo images, from the mapping and panoramic cameras, covered swaths totaling 16% of the lunar surface, at resolutions of approximately 5 m. The Lunar Reconnaissance Orbiter -- launched in June 2009 to a polar orbit -- carries a suite of instruments that will revolutionize lunar remote sensing, including the identification and characterization of lava tubes. The Lunar Reconnaissance Orbiter Camera (LROC) system includes a multi-spectral wide-angle camera with a resolution of 70 m, allowing a comprehensive survey of the entire lunar surface. The LROC narrow-angle camera is providing targeted images at resolutions of 0.5 - 2 m, including stereo coverage, which should allow detection of tube entrances and breakdown structures. The Lunar Orbiter Laser Altimeter is producing a global topographic map with a vertical resolution of 1 m and a horizontal resolution of 50 m. These data will be critical to understanding lava dynamics and tube emplacement.

Allen, Carlton C.↗

Analog Environments for Venus Aerosol Instrument and Mission Concept Testing

The most common hypothesis discussed for life on Venus is a habitat in the dense, persistent clouds, which are primarily sulfuric acid with a secondary water component. Although Earth lacks a complete Venus analog environment, several partial analogs exist which can inform our understanding of the requirements for cloud habitability as well as serving as potential testbeds for future Venus instruments or missions. Earth’s tropospheric clouds have larger particles, more water, and shorter lifetimes than Venus’s; however, they have a partial overlap in pressure and temperature, and are our only example of an airborne habitat.They can inform how particle residence time and microbial generation time constrain habitable atmospheric regions. On the biological side, this includes available nutrients and energy, environmental stressors such as radiation, osmotic potential and acidity, and time spent in protective but inactive forms (endospores, cysts, akinetes); on the physical side, gravity, air density and viscosity, thermal lofting, gravity waves, scavenging due to precipitation, and other airflow dynamics, as well as effective particle radius and electrical charge. They can also enable sampling strategy tests for airborne life detection, ranging from10 2 to10 8 viable cells/mL (10 -3 to 10 -9 cells/particle). Conversely, Earth's stratospheric sulfate layer lacks an active microbial presence, and is at a colder and less dense altitude but has partially analogous values in terms of H2SO4 and water concentration, particle size, and number density. These aerosols are accessible to aircraft and high-altitude balloons. Even more accessible potential analogs include sea spray, which overlaps with the smaller (haze) particle sizes and densities at Venus and contains trace organic constituents; marine fog, which at higher densities can match Venus’s larger (Mode 2) particle properties and contains a range of trace compounds including ammonia, phosphates, sulfates, and nitrates; and spray from acidic hydrothermal systems, which can cover a wide variety of sizes, densities, and chemical compositions. These analogs could improve the development of Venus cloud sampling instruments or sondes, including field tests of material compatibility, particle capture efficiency, and analysis sensitivity.

Analog↗