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DOE OSTI2014

A geometric theory for Lévy distributions

Lévy distributions are of prime importance in the physical sciences, and their universal emergence is commonly explained by the Generalized Central Limit Theorem (CLT). However, the Generalized CLT is a geometry-less probabilistic result, whereas physical processes usually take place in an embedding space whose spatial geometry is often of substantial significance. In this paper we introduce a model of random effects in random environments which, on the one hand, retains the underlying probabilistic structure of the Generalized CLT and, on the other hand, adds a general and versatile underlying geometric structure. Based on this model we obtain geometry-based counterparts of the Generalized CLT, thus establishing a geometric theory for Lévy distributions. The theory explains the universal emergence of Lévy distributions in physical settings which are well beyond the realm of the Generalized CLT.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2014

Geometrical aspects on the dark matter problem

In the present paper we apply Nash’s theory of perturbative geometry to the study of dark matter gravity in a higher-dimensional space–time. It is shown that the dark matter gravitational perturbations at local scale can be explained by the extrinsic curvature of the standard cosmology. In order to test our model, we use a spherically symmetric metric embedded in a five-dimensional bulk. As a result, considering a sample of 10 low surface brightness and 6 high surface brightness galaxies, we find a very good agreement with the observed rotation curves of smooth hybrid alpha-HI measurements. - Highlights: • The metric perturbation and the embedding lead naturally to a “brane-world”-like higher dimensional structure. • Nash’s theorem as a cornerstone of the formation of geometrical structures. • The dark matter gravitational perturbations at local scale can be explained by the extrinsic curvature. • A good agreement was found with the observed rotation curves of smooth hybrid alpha-HI measurements.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2014

The extended Bloch representation of quantum mechanics and the hidden-measurement solution to the measurement problem

A generalized Bloch sphere, in which the states of a quantum entity of arbitrary dimension are geometrically represented, is investigated and further extended, to also incorporate the measurements. This extended representation constitutes a general solution to the measurement problem, inasmuch it allows to derive the Born rule as an average over hidden-variables, describing not the state of the quantum entity, but its interaction with the measuring system. According to this modelization, a quantum measurement is to be understood, in general, as a tripartite process, formed by an initial deterministic decoherence-like process, a subsequent indeterministic collapse-like process, and a final deterministic purification-like process. We also show that quantum probabilities can be generally interpreted as the probabilities of a first-order non-classical theory, describing situations of maximal lack of knowledge regarding the process of actualization of potential interactions, during a measurement. - Highlights: • An extended Bloch representation of quantum measurements is given. • Quantum measurements are explained in terms of hidden-measurement interactions. • Quantum measurements are explained as tripartite processes. • The Born rule results from a universal average, over all possible measurement processes.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2018

Two diverse models of embedding class one

Highlights: •The embedding of curved spacetime in higher-dimensional flat spacetime is discussed. •The focus is on two diverse models of embedding class one. •The first model is a complete wormhole solution including the junction conditions. •The second is a new model to explain the flat galactic rotation curves without the need for dark matter. -- Abstract: Embedding theorems have continued to be a topic of interest in the general theory of relativity since these help connect the classical theory to higher-dimensional manifolds. This paper deals with spacetimes of embedding class one, i.e., spacetimes that can be embedded in a five-dimensional flat spacetime. These ideas are applied to two diverse models, a complete solution for a charged wormhole admitting a one-parameter group of conformal motions and a new model to explain the flat rotation curves in spiral galaxies without the need for dark matter.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2018

More on the new large D limit of matrix models

In this paper, we extend the recent analysis of the new large D limit of matrix models to the cases where the action contains arbitrary multi-trace interaction terms as well as to arbitrary correlation functions. We discuss both the cases of complex and Hermitian matrices, with U(N){sup 2}×O(D) and U(N)×O(D) symmetries respectively. In the latter case, the new large D limit is consistent for planar diagrams; at higher genera, it crucially requires the tracelessness condition. For similar reasons, the large N limit of tensor models with reduced symmetries is typically inconsistent already at leading order without the tracelessness condition. We also further discuss some interesting properties of purely bosonic models pointed out recently and explain that the standard argument predicting a non-trivial IR behavior in fermionic models à la SYK does not work for bosonic models. Finally, we explain that the new large D scaling is consistent with linearly realized supersymmetry.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2018

Advances in the calculation of optical properties in superlattices; novel insights derived from the theory of finite periodic systems

Highlights: • Truly quantum calculation of optical properties in superlattices. • An approach that goes beyond the abilities of the current approaches in the literature. • Accurate calculations based on bona fide superlattice eigenvalues and eigenfunctions. • Accurate calculation of optical-transition matrix elements and new selection rules. • Optical spectra of AlGaAs and InGaN superlattices agree with experimental results. • Optical spectra features of blue diodes faithfully reproduced and fully explained. • Unexplained lines in high-resolution spectra assigned to surface and edge states. - Abstract: Using the genuine superlattice eigenvalues and eigenfunctions, and their parity symmetries, new transition selection rules are derived, and a novel approach for the explicit calculation of the optical spectra of periodic structures presented. The optical response and infrared spectra published in the last 40 years for (In, Ga)N and (Al, Ga)As superlattices, with different number of unit cells n (varying from ∼10 to ∼400) and barrier and valley widths in the range of 2.5 nm to 40 nm, and different properties have been revisited. Among the various examples discussed here, we study the narrow peaks clustered in groups reported in high resolution spectra of blue-emitting devices, that could not be explained until now. These properties are faithfully reproduced and fully understood. We show that these effects are related to and depend on the surface energy levels and their tuneable detachment. We shown also that the optical transitions experimentally observed, but forbidden by the current optical physics, are really allowed transitions. Since the number of energy eigenvalues E{sub μ,ν}{sup r,v} and eigenfunctions Ψ{sub μ{sup ′},ν{sup ′r,v}}(z) is now much greater, the number of allowed matrix-elements can be extremely large (of the order of n{sup 2} n{sub c}n{sub v}∕2, with n{sub c} and n{sub v} the number of subbands in the conduction and valence bands). We show that this number can be reduced by using the eigenfunctions’ symmetries, which are behind the parity symmetry selection rules and the leading order selection rules. These rules reduce the number of matrix-elements evaluations from ∼n{sup 2}n{sub c}n{sub v}∕2 to ∼nn{sub c}n{sub v}∕2, i.e., depending on the superlattice, from about 1000 to 100. We discuss also a third rule, that collects the contributions of the surface and edge states, reducing the number of transitions even more to N{sub s}≤n{sub c}n{sub v}. With these rules, the main peaks are conserved and their number practically matches that of the real spectrum. Excellent agreements are found with experimental results.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2021

Adiabatic quantum decoherence in many non-interacting subsystems induced by the coupling with a common boson bath

Highlights: • System–environment quantum correlation: a main solid state NMR decoherence channel. • Non-separable system–environment model yields realistic spin decoherence rates. • New open quantum system approach explains irreversible decay of refocused NMR echoes. • Adiabatic quantum decoherence is inherently irreversible and eigen-selective. This work addresses adiabatic quantum decoherence of many-body spin systems coupled with a boson field in the framework of open quantum systems theory. We generalize the traditional spin-boson model by considering a system–environment interaction Hamiltonian that represents a partition of non-interacting subsystems and highlights the collective correlation that appears exclusively due to the coupling with a common environment. Remarkably, this simple, exactly solvable model encompasses relevant aspects of a many-body open quantum system and features the subtle quantum effects that arise when the size scales up to a macroscopic level. We derive an analytical expression for the time dependence of the density matrix elements (in the preferred basis) without assuming coarse-graining. The resulting decoherence function is eigen-selective and is a complex exponential whose exponent has a real part that introduces a decay similar to that in the spin-boson model. On the contrary, the imaginary part depends on the quantum numbers and geometry of the whole partition and does not reflect the system temperature. Motivated by decoherence in solid-state NMR, and in search of realistic numerical estimations, we apply the theoretical results to a partition of dipole-coupled spin pairs in contact with a common phonon bath, using typical parameters of hydrated salts. The proposal allows estimating the decoherence time scale in terms of the system physical constants: sound velocity and eigenvalue distribution width. As a significant novelty, the decoherence function phase depends on the eigenvalue distribution throughout the sample. It plays the leading role, overshadowing the mechanism associated with the bath thermal state. Finally, we apply the formalism to describe decoherence in the “magic echo” NMR reversal experiment. We find that the system–environment correlation explains the origin of irreversibility, and both the decoherence rate value and its dependence on the dipolar frequency, are remarkably similar to the experiment.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2017

A re-examination of the catalyst activation and temperature hysteresis in methane combustion on Pt/Al 2 O 3

Methane combustion is an industrially important reaction that has been extensively studied. Supported Pt catalysts on Al 2 O 3 exhibit excellent activity in this reaction owing to their Csingle bondH bond activation capability. Pt/Al 2 O 3 catalyst remains almost inactive until the conversion increases abruptly at the ignition temperature (T ign ). The temperature hysteresis behavior has been reported, where after ignition, as the temperature is lowered below T ign , the catalyst continues to exhibit high activity. The activation has been previously attributed to the removal of adsorbed oxygen on Pt sites at T ign , while the hysteresis has been explained by the local temperature elevation caused by the reaction heat. Here in this work, this behavior was re-examined at a small scale in a fixed-bed flow reactor over a range of pre-treatment and reaction mixture conditions. Low-temperature activation was achieved by pre-treating the catalyst with CH 4 . Carefully designed control experiments yielded observations that cannot be rationalized by the existing theory, suggesting that it is incomplete. Based on these new results, we propose a more comprehensive theory describing the active sites in the oxidation reaction as methane-derived adsorbed carbon active sites, and their formation as the controlling factor in the catalyst activation. The hysteresis can be better explained by the enhanced stability of active sites under reaction conditions. In situ infrared spectroscopy and mass spectrometry studies were conducted in a flow cell, providing strong evidence supporting the theory. The dissociative adsorption of CH 4 was studied under a variety of pre-treatment conditions, and proved to be responsible for the active site formation. The fundamental understanding obtained in this work contributes significant insights to the understanding of reaction mechanism.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2017

Kinetics of hydrogenation and hydrogenolysis of 2,5-dimethylfuran over noble metals catalysts under mild conditions

Ketones, derived via the selective ring-opening hydrogenation of biomass-derived furanic compounds, are useful synthons for the production of fuels and lubricant. In this study, 2,5-dimethylfuran (DMF) was used as a model to investigate the liquid-phase hydrogenation of substituted furans over noble metals. The activity and selectivity of carbon-supported Pt, Pd, Rh, and Ru were compared under identical conditions. Pt/C exhibited the highest activity and selectivity for C—O bond hydrogenolysis, whereas all other catalysts exhibited a high selectivity for ring hydrogenation to form 2,5-dimethyltetrahydrofuran. The reaction kinetics were measured in detail for liquid-phase hydrogenation of DMF over Pt/C, under conditions that excluded the effects of internal or external mass transfer (333 K–363 K, p H2 = 0.41–0.69 MPa). A reaction mechanism is proposed to explain the parallel formation of 2,5-dimethyltetrahydrofuran, 2-hexanone, and 2-hexanol. This mechanism is consistent with what is known about the adsorption of DMF on Pt, and explains why ring opening occurs directly from adsorbed DMF and not via secondary reaction of 2,5-dimethyltetrahydrofuran. The kinetics of DMF hydrogenation are well represented by the mechanism and the assumption of non-competitive adsorption of organic species and hydrogen atoms. The step involving C—O bond cleavage of the aromatic ring had the highest activation energy, 45.4 kJ/mol, and is favored over the steps involving C═C bond hydrogenation of the furan ring at elevated temperatures and low p H2 . Finally, the reaction kinetics reveal that DMF hydrogenation can be tuned to produce 2-hexanone with 92% selectivity at 100% conversion under relatively mild liquid phase conditions (0.41 MPa, 393 K).

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2020

Field-assisted suppression of coke in the methane steam reforming reaction

Electric fields were applied to an unsupported nickel catalyst during methane steam reforming with a low steam-to-methane ratio of 2:1. As expected, catalysts operated in standard mode (without an applied field) experienced high levels of coking. Coke formation was almost completely suppressed by the application of a positive electric field. This is explained using density functional theory computations which indicate that positive electric fields (1) increase the affinity for water adsorption on the surface, thereby increasing the amount of oxygen available to remove carbon, (2) stabilize individual carbon fragments, thus reducing their tendency to polymerize, and (3) increase the oxygen storage capabilities of the catalyst by inducing sub-surface oxygen formation and bulk oxidation.

Gray, Jake T.↗
DOE OSTI2022

Luminescence dosimetry for evaluation of the external exposure in Metlino, upper Techa River valley, Southern Urals, Russia: Analysis of new results

Luminescence dosimetry was applied in the former settlement of Metlino, Southern Urals, Russia as part of a full-scale study to validate the Techa River Dosimetry System (TRDS) 2016 for the upper Techa River region. The village, which was evacuated in 1956, was located 7 km downstream of the release point of liquid radioactive waste by the Mayak plutonium facility. Several brick samples were taken from north-eastern and south-eastern walls of the granary, facing the former Techa river shoreline and floodplain. Samples were all taken at the same height and measured at different depths into the brick. For the majority of brick samples, good Optically Stimulated Luminescence properties of the quartz grains were observed. In some cases, however, strong levels of sensitization and/or signal recuperation were encountered which necessitated adjustment in the measurement protocols. Anthropogenic doses in bricks varied from 1.5 to 6.6 Gy and the horizontal profiles along both walls showed significant variation, which is explained on a qualitative basis. Further, a dose depth profile is observed for selected samples, which is different from the dose depth profile measured and simulated for samples from the north-western wall of the granary in previous studies. This is qualitatively explained by the differences in source configuration.

61 RADIATION PROTECTION AND DOSIMETRY↗
DOE OSTI2013

S5 0716+714: GeV variability study

The GeV observations by Fermi-LAT give us the opportunity to characterize the high-energy emission (100 MeV–300 GeV) variability properties of the BL Lac object S5 0716+714. In this study, we performed flux and spectral analysis of more than 3 year long (August 2008 to April 2012) Fermi-LAT data of the source. During this period, the source exhibits two different modes of flux variability with characteristic timescales of ~75 and ~140 days, respectively. In addition, we also notice that the flux variations are characterized by a weak spectral hardening. The GeV spectrum of the source shows a clear deviation from a simple power law, and is better explained by a broken power law. Similar to other bright Fermi blazars, the break energy does not vary with the source flux during the different activity states. Finally, we discuss several possible scenarios to explain the observed spectral break.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2012

Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase

The biphenyl dioxygenase of Burkholderia xenovorans LB400 (BphAE{sub LB400}) is a Rieske-type oxygenase that catalyzes the stereospecific oxygenation of many heterocyclic aromatics including dibenzofuran. In a previous work, we evolved BphAE{sub LB400} and obtained BphAE{sub RR41}. This variant metabolizes dibenzofuran and 2-chlorodibenzofuran more efficiently than BphAE{sub LB400}. However, the regiospecificity of BphAE{sub RR41} toward these substrates differs. Dibenzofuran is metabolized principally through a lateral dioxygenation whereas 2-chlorodibenzofuran is metabolized principally through an angular dioxygenation. In order to explain this difference, we examined the crystal structures of both substrate-bound forms of BphAE{sub RR41} obtained under anaerobic conditions. This structure analysis, in combination with biochemical data for a Ser283Gly mutant provided evidences that the substrate is compelled to move after oxygen-binding in BphAE{sub RR41}:dibenzofuran. In BphAE{sub RR41}:2-chlorodibenzofuran, the chlorine atom is close to the side chain of Ser283. This contact is missing in the BphAE{sub RR41}:dibenzofuran, and strong enough in the BphAE{sub RR41}:2-chlorodibenzofuran to help prevent substrate movement during the catalytic reaction.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2012

IGF-I enhances cellular senescence via the reactive oxygen species-p53 pathway

Highlights: Black-Right-Pointing-Pointer Cellular senescence plays an important role in tumorigenesis and aging process. Black-Right-Pointing-Pointer We demonstrated IGF-I enhanced cellular senescence in primary confluent cells. Black-Right-Pointing-Pointer IGF-I enhanced cellular senescence in the ROS and p53-dependent manner. Black-Right-Pointing-Pointer These results may explain the underlying mechanisms of IGF-I involvement in tumorigenesis and in regulation of aging. -- Abstract: Cellular senescence is characterized by growth arrest, enlarged and flattened cell morphology, the expression of senescence-associated {beta}-galactosidase (SA-{beta}-gal), and by activation of tumor suppressor networks. Insulin-like growth factor-I (IGF-I) plays a critical role in cellular growth, proliferation, tumorigenesis, and regulation of aging. In the present study, we show that IGF-I enhances cellular senescence in mouse, rat, and human primary cells in the confluent state. IGF-I induced expression of a DNA damage marker, {gamma}H2AX, the increased levels of p53 and p21 proteins, and activated SA-{beta}-gal. In the confluent state, an altered downstream signaling of IGF-I receptor was observed. Treatment with a reactive oxygen species (ROS) scavenger, N-acetylcystein (NAC) significantly suppressed induction of these markers, indicating that ROS are involved in the induction of cellular senescence by IGF-I. In p53-null mouse embryonic fibroblasts, the IGF-I-induced augmentation of SA-{beta}-gal and p21 was inhibited, demonstrating that p53 is required for cellular senescence induced by IGF-I. Thus, these data reveal a novel pathway whereby IGF-I enhances cellular senescence in the ROS and p53-dependent manner and may explain the underlying mechanisms of IGF-I involvement in tumorigenesis and in regulation of aging.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2013

Induction of mitochondrial biogenesis and respiration is associated with mTOR regulation in hepatocytes of rats treated with the pan-PPAR activator tetradecylthioacetic acid (TTA)

Highlights: Black-Right-Pointing-Pointer We investigated mechanisms of mitochondrial regulation in rat hepatocytes. Black-Right-Pointing-Pointer Tetradecylthioacetic acid (TTA) was employed to activate mitochondrial oxidation. Black-Right-Pointing-Pointer Mitochondrial biogenesis and respiration were induced. Black-Right-Pointing-Pointer It was confirmed that PPAR target genes were induced. Black-Right-Pointing-Pointer The mechanism involved activation mTOR. -- Abstract: The hypolipidemic effect of peroxisome proliferator-activated receptor (PPAR) activators has been explained by increasing mitochondrial fatty acid oxidation, as observed in livers of rats treated with the pan-PPAR activator tetradecylthioacetic acid (TTA). PPAR-activation does, however, not fully explain the metabolic adaptations observed in hepatocytes after treatment with TTA. We therefore characterized the mitochondrial effects, and linked this to signalling by the metabolic sensor, the mammalian target of rapamycin (mTOR). In hepatocytes isolated from TTA-treated rats, the changes in cellular content and morphology were consistent with hypertrophy. This was associated with induction of multiple mitochondrial biomarkers, including mitochondrial DNA, citrate synthase and mRNAs of mitochondrial proteins. Transcription analysis further confirmed activation of PPAR{alpha}-associated genes, in addition to genes related to mitochondrial biogenesis and function. Analysis of mitochondrial respiration revealed that the capacity of both electron transport and oxidative phosphorylation were increased. These effects coincided with activation of the stress related factor, ERK1/2, and mTOR. The protein level and phosphorylation of the downstream mTOR actors eIF4G and 4E-BP1 were induced. In summary, TTA increases mitochondrial respiration by inducing hypertrophy and mitochondrial biogenesis in rat hepatocytes, via adaptive regulation of PPARs as well as mTOR.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2013

High extensibility of stress fibers revealed by in vitro micromanipulation with fluorescence imaging

Highlights: •We isolate contractile stress fibers from vascular smooth muscle cells. •We measure the extensibility of individual stress fibers. •We present the first direct evidence that individual stress fibers are highly extensible. •We quantitatively determine the local strain along the length of stress fibers. •The high extensibility we found is beyond that explained by a conventional model. -- Abstract: Stress fibers (SFs), subcellular bundles of actin and myosin filaments, are physically connected at their ends to cell adhesions. The intracellular force transmitted via SFs plays an essential role in cell adhesion regulation and downstream signaling. However, biophysical properties intrinsic to individual SFs remain poorly understood partly because SFs are surrounded by other cytoplasmic components that restrict the deformation of the embedded materials. To characterize their inherent properties independent of other structural components, we isolated SFs from vascular smooth muscle cells and mechanically stretched them by in vitro manipulation while visualizing strain with fluorescent quantum dots attached along their length. SFs were elongated along their entire length, with the length being approximately 4-fold of the stress-free length. This surprisingly high extensibility was beyond that explained by the tandem connection of actin filaments and myosin II bipolar filaments present in SFs, thus suggesting the involvement of other structural components in their passive biophysical properties.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

Time-dependent inhibitory effects of cGMP-analogues on thrombin-induced platelet-derived microparticles formation, platelet aggregation, and P-selectin expression

Highlights: • We investigated the impact of cyclic nucleotide analogues on platelet activation. • Different time dependence were found for inhibition of platelet activation. • Additive effect was found using PKA- and PKG-activating analogues. • Our results may explain some of the discrepancies reported for cNMP signalling. - Abstract: In platelets, nitric oxide (NO) activates cGMP/PKG signalling, whereas prostaglandins and adenosine signal through cAMP/PKA. Cyclic nucleotide signalling has been considered to play an inhibitory role in platelets. However, an early stimulatory effect of NO and cGMP-PKG signalling in low dose agonist-induced platelet activation have recently been suggested. Here, we investigated whether different experimental conditions could explain some of the discrepancy reported for platelet cGMP-PKG-signalling. We treated gel-filtered human platelets with cGMP and cAMP analogues, and used flow cytometric assays to detect low dose thrombin-induced formation of small platelet aggregates, single platelet disappearance (SPD), platelet-derived microparticles (PMP) and thrombin receptor agonist peptide (TRAP)-induced P-selectin expression. All four agonist-induced platelet activation phases were blocked when platelets were costimulated with the PKG activators 8-Br-PET-cGMP or 8-pCPT-cGMP and low-doses of thrombin or TRAP. However, extended incubation with 8-Br-PET-cGMP decreased its inhibition of TRAP-induced P-selectin expression in a time-dependent manner. This effect did not involve desensitisation of PKG or PKA activity, measured as site-specific VASP phosphorylation. Moreover, PKG activators in combination with the PKA activator Sp-5,6-DCL-cBIMPS revealed additive inhibitory effect on TRAP-induced P-selectin expression. Taken together, we found no evidence for a stimulatory role of cGMP/PKG in platelets activation and conclude rather that cGMP/PKG signalling has an important inhibitory function in human platelet activation.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

The glucagon-like peptide 1 receptor agonist enhances intrinsic peroxisome proliferator-activated receptor γ activity in endothelial cells

Highlights: • PPARγ activation was involved in the GLP-1-mediated anti-inflammatory action. • Exendin-4 enhanced endogenous PPARγ transcriptional activity in HUVECs. • H89, a PKA inhibitor, abolished GLP-1-induced PPARγ enhancement. • The anti-inflammatory effects of GLP-1 may be explained by PPARγ activation. - Abstract: Recent studies have suggested glucagon-like peptide-1 (GLP-1) signaling to exert anti-inflammatory effects on endothelial cells, although the precise underlying mechanism remains to be elucidated. In the present study, we investigated whether PPARγ activation is involved in the GLP-1-mediated anti-inflammatory action on endothelial cells. When we treated HUVEC cells with 0.2 ng/ml exendin-4, a GLP-1 receptor agonist, endogenous PPARγ transcriptional activity was significantly elevated, by approximately 20%, as compared with control cells. The maximum PPARγ activity enhancing effect of exendin-4 was observed 12 h after the initiation of incubation with exendin-4. As H89, a PKA inhibitor, abolished GLP-1-induced PPARγ enhancement, the signaling downstream from GLP-1 cross-talk must have been involved in PPARγ activation. In conclusion, our results suggest that GLP-1 has the potential to induce PPARγ activity, partially explaining the anti-inflammatory effects of GLP-1 on endothelial cells. Cross-talk between GLP-1 signaling and PPARγ activation would have major impacts on treatments for patients at high risk for cardiovascular disease.

60 APPLIED LIFE SCIENCES↗