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At least 1,225 records · Page 68

Changes in the Molar Ellipticities of HEWL Observed by Circular Dichroism and Quantitated by Time Resolved Fluorescence Anisotropy Under Crystallizing Conditions

Fluid models for simple colloids predict that as the protein concentration is increased, crystallization should occur at some sufficiently high concentration regardless of the strength of attraction. However, empirical measurements do not fully support this assertion. Measurements of the second virial coefficient (B22) indicate that protein crystallization occurs only over a discrete range of solution parameters. Furthermore, observations of a strong correlation between protein solubility and B22, has led to an ongoing debate regarding the relationship between the two. Experimental work in our lab, using Hen Egg White Lysozyme (HEWL), previously revealed that the rotational anisotropy of the protein under crystallizing conditions changes systematically with pH, ionic strength and temperature. These observations are now supported by recent work revealing that small changes in the molar ellipticity also occur systematically with changes in ionic strength and temperature. This work demonstrates that under crystallization conditions, the protein native state is characterized by a conformational heterogeneity that may prove fundamental to the relationship between protein crystallization and protein solubility.

Sumida, John↗

Experimental Evaluation of Analytical Models for the Inertias and Natural Frequencies of Fuel Sloshing in Circular Cylindrical Tanks

A correlation is presented of experimental measurement and analytical prediction of free, planar oscillations of a tank of mercury hung on a pendulum. Measured frequencies are presented for the slosh mode and the pendulum-tank mode, and time-history wave forms are included for qualitative comparisons. The sloshing is represented analytically by a single pendulum or a spring-bob, and the size and location of their inertias are determined by matching forces and moments on the tank surface with linearized hydrodynamic theory for inviscid fluids. It is concluded that such analytical models are identical for small displacements in the present application (as they are in simpler applications) and that they provide excellent simulation of the stiffness and in ertia terms for fuel sloshing.

Warner, Robert W.↗

Numerical Study of Flow Past a Circular Cylinder Using RANS, Hybrid RANS/LES and PANS Formulations

Two multiscale type turbulence models are implemented in the PAB3D solver. The models are based on modifying the Reynolds Averaged Navier-Stokes (RANS) equations. The first scheme is a hybrid RANS/LES model utilizing the two-equation (k(sub epsilon)) model with a RANS/LES transition function dependent on grid spacing and the computed turbulence length scale. The second scheme is a modified version of the partially averaged Navier-Stokes (PANS) model, where the unresolved kinetic energy parameter (f(sub k)) is allowed to vary as a function of grid spacing and the turbulence length scale. Solutions from these models are compared to RANS results and experimental data for a stationary and rotating cylinder. The parameter f(sub k) varies between zero and one and has the characteristic to be equal to one in the viscous sub layer, and when the RANS turbulent viscosity becomes smaller than the LES viscosity. The formulation, usage methodology, and validation example are presented to demonstrate the enhancement of PAB3D's time-accurate and turbulence modeling capabilities. The models are compared to RANS results and experimental data for turbulent separated flows (TS) and laminar separated flows (LS) around stationary and rotating cylinders. For a stationary cylinder, the TS case is accurately simulated using the general two-equation k(sub epsilon) turbulence model (eddy-viscosity model). PAB3D accurately predicts the drag coefficient (CD), lift coefficient (CL) and the Strouhal number (St). The LS case was a challenge for the RANS computation with an eddy-viscosity turbulence model. The RANS/LES and PANS performed well and showed marked improvements over the RANS solution. The modified PANS model was the most accurate. For the rotating cylinder, the spin ratio varied from zero to one, and the PANS results were in good agreement with published experimental data. RANS/LES and PANS capture both temporal and spatial fluctuations and produce large-scale structures that do not occur in the RANS simulation. The current results show promise for the capability of PANS in simulating unsteady and complex flow phenomena.

Elmiligui, Alaa A.↗

Investigation of a Half-Conical Scoop Inlet Mounted at Five Alternate Circumferential Locations around a Circular Fuselage Pressure-Recovery Results at a Mach Number 2.01

The effects of inlet circumferential position around the fuselage on the characteristics of a half-conical scoop inlet having a 24.6deg half-angle cone have been investigated in the langley 4- by 4-foot supersonic pressure tunnel. Pressure-recovery results have been obtained at a Mach number of 2.01 for a fixed boundary-layer-bleed height which was 60 percent of the boundary-layer thickness at an angle of attack of 0deg, and for cowling position parameters of 42.4deg and 38.0deg. inlet had a capture area equal to 24.9 percent of the basic-fuselage frontal area. The angle of attack was varied from 0deg to 12deg. The most favorable pressure-recovery characteristics at angles of attack were obtained with the Inlet located on the bottom of the fuselage where the maximum recovery increased from a value of 81 percent at an angle of attack of 0deg to 87 percent at 12deg. In general, the pressure recovery decreased with increasing angle of attack for all other inlet locations. At a given angle of attack the pressure recovery decreased as the inlet location was progressively moved from the bottom to the top of the fuselage. Stable subcritical operation of the inlet with nearly constant pressure recovery was obtained for inlet mass-flow ratios from 1.0 to about 0.76 at an angle of attack of 0deg with the central body in the design position.

Hasel, Lowell E.↗

The Problem of Torsion in Prismatic Members of Circular Segmental Cross Section

The problem is solved by approximation, by setting up a function complying with the differential equation of the stress function, and determining the coefficients appearing in it in such a way that the boundary condition is fulfilled as nearly as possible. For the semicircle, for which the solution is known, the method yields very accurate values; the approximated stress distribution is in good agreement with the accurately computed distribution. Stress and strain measurements indicate that the approximate solution is in sufficiently exact agreement with reality for segmental cross sections.

Weigand, A.↗

Light Scattering by an Infinite Circular Cylinder Immersed in an Absorbing Medium

Analytic solutions are developed for the single-scattering properties of an infinite dielectric cylinder embedded in an absorbing medium with normal incidence, which include extinction, scattering and absorption efficiencies, the scattering phase function, and the asymmetry factor. The extinction and scattering efficiencies are derived by the near-field solutions at the surface of the particle. The normalized scattering phase function is obtained by use of the far-field approximation. Computational results show that while the absorbing medium significantly reduces the scattering efficiency, it has little effect on absorption efficiency. The absorbing medium can significantly change the conventional phase function. The absorbing medium also strongly affects the polarization of the scattered light. However, for large absorbing particles the degrees of polarization change little with the medium s absorption. This implies that if the transmitting lights are strongly weakened inside the particle the scattered polarized lights can be used to identify objects even when the absorption property of the host medium is unknown, which is very important for both active and passive remote sensing.

Sun, W.↗

An Isolated Circular Synthetic Jet in Cross-Flow at Low Momentum-Flux Ratio

A joint experimental and computational investigation was carried out for a round synthetic jet issuing normal to a turbulent boundary layer at a momentum-flux ratio of one. Distributions of velocity and turbulence intensity were measured by hot-wire anemometry. Numerical results were obtained using unsteady Reynolds-averaged Navier-Stokes (URANS) computations. Time and phase-averaged flow properties were compared on the cross sectional plane at x/D = 0.53, 5 and 10 as well as on the axial plane of symmetry. Overall, the numerical results agreed well with the experimental data. CFD predicted a somewhat larger velocity deficit in regions of low-momentum fluid pulled up from the boundary layer. Phase- averaged velocity contours at the plane of symmetry indicated good match between experiments and CFD regarding the size and the position of the periodic flow structure. However, some differences occurred in details such as the shape and inclination of the low-speed flow structure.

Milanovic, Ivana M.↗

Analysis and Simulation for a Spotlight-Mode Aircraft SAR in Circular Flight Path

A wide azimuth beam SAR can offer higher resolution or wider azimuth viewing angle; two factors that help better characterize the backscattering property of targets for various science applications. One disadvantage of wide beam SAR is that a much higher pulse repetition frequency (PRF) is usually required since PRF is proportional to the radar beam angle. This problem can be resolved using a spotlight-mode concept: steering a narrow beam SAR to a fixed spot on the ground. The drawback of a spotlight-mode SAR is its limited coverage. A conventional spotlight-mode SAR operates along a straight line path as shown in Figure 1. It can be shown that spotlight-mode SAR that follows a straight line path has difficulty in achieving the ultimate resolution of lambda/4. It also cannot utilize the full 180 degree of azimuth viewing angle that can be attained only when the synthetic aperture length approaches infinity.

Jin, Michael Y.↗

Streamwise Vortices on the Convex Surfaces of Circular Cylinders and Turbomachinery Blading

In assessing the results please recall that the Mach number regimes and model geometries differ considerably. Selection of the radius of curvature at the 10% chord location is consistent but arbitrary, although it does seem representative for most blades and gives a good fit for the results. Measured spanwise wavelengths of the periodic vortex arrays on blading are predicted well by the Kestin and Wood theory. If this behavior is at all common it could have implications for turbine aerodynamic and blade cooling design. The outcome is to establish that organized streamwise vorticity may occur more frequently on convex surfaces, such as turbine blade suction surfaces, than hitherto appreciated. Investigations and predictions of flow behavior should be extended to encompass that possibility.

Gostelow, Paul↗

Versatile platform for nanotechnology based on circular permutations of chaperonin protein

The present invention provides chaperonin polypeptides which are modified to include N-terminal and C-terminal ends that are relocated from the central pore region to various different positions in the polypeptide which are located on the exterior of the folded modified chaperonin polypeptide. In the modified chaperonin polypeptide, the naturally-occurring N-terminal and C-terminal ends are joined together directly or with an intervening linker peptide sequence. The relocated N-terminal or C-terminal ends can be covalently joined to, or bound with another molecule such as a nucleic acid molecule, a lipid, a carbohydrate, a second polypeptide, or a nanoparticle. The modified chaperonin polypeptides can assemble into double-ringed chaperonin structures. Further, the chaperonin structures can organize into higher order structures such as nanofilaments or nanoarrays which can be used to produce nanodevices and nanocoatings.

Paavola, Chad D.↗

Background Oriented Schlieren Applied to Study Shock Spacing in a Screeching Circular Jet

Background oriented schlieren (BOS) is a recent development of the schlieren and shadowgraph methods. The BOS technique has the ability to provide visualizations of the density gradient in both the axial and radial directions. The resultant magnitude of the density gradients allows for comparison with shadowgraph images. This paper first compares data obtained by the BOS and shadowgraph techniques at identical conditions in a free jet. The patterns and spacing of the shock trains obtained by the two techniques are found to be consistent with one another. This provides confidence in the shock spacing measurement by the BOS technique. Due to its simpler setup, BOS is then applied to investigate the shock spacing associated with the screech phenomenon, especially during stage jumps. Screech frequencies from a 37.6 mm convergent nozzle, as a function of jet Mach number (M(sub j)), are shown to exhibit various stages. As many as eight stages are identified with the present nozzle over the range 1.0 < M(sub j) <1.7. BOS images are acquired at various screech conditions and the shock spacing is examined as a function of M(sub j).

Clem, Michelle M.↗

Analysis of an Aircraft Honeycomb Sandwich Panel with Circular Face Sheet/Core Disbond Subjected to Ground-Air Pressurization

The ground-air pressurization of lightweight honeycomb sandwich structures caused by alternating pressure differences between the enclosed air within the honeycomb core and the ambient environment is a well-known and controllable loading condition of aerospace structures. However, initial face sheet/core disbonds intensify the face sheet peeling effect of the internal pressure load significantly and can decrease the reliability of the sandwich structure drastically. Within this paper, a numerical parameter study was carried out to investigate the criticality of initial disbonds in honeycomb sandwich structures under ground-air pressurization. A fracture mechanics approach was used to evaluate the loading at the disbond front. In this case, the strain energy release rate was computed via the Virtual Crack Closure Technique. Special attention was paid to the pressure-deformation coupling which can decrease the pressure load within the disbonded sandwich section significantly when the structure is highly deformed.

Rinker, Martin↗

Circular-Orbit Maintenance Strategies for Primitive Body Orbiters

For missions to smaller primitive bodies, solar radiation pressure (SRP) is a significant perturbation to Keplerian dynamics. For most orbits, SRP drives large oscillations in orbit eccentricity, which leads to large perturbations from the irregular gravity field at periapsis. Ultimately, chaotic motion results that often escapes or impacts that body. This paper presents an orbit maintenance strategy to keep the orbit eccentricity small, thus avoiding the destabilizing secondary interaction with the gravity field. An estimate of the frequency and magnitude of the required maneuvers as a function of the orbit and body parameters is derived from the analytic perturbation equations.

comet↗

Analysis of Near-field of Circular Aperture Antennas with Application to Study of High Intensity Radio Frequency (HIRF) Hazards to Aviation from JPL/NASA Deep Space Network Antennas

This work includes a simplified analysis of the radiated near to mid-field from JPL/NASA Deep Space Network (DSN) reflector antennas and uses an averaging technique over the main beam region and beyond for complying with FAA regulations in specific aviation environments. The work identifies areas that require special attention, including the implications of the very narrow beam of the DSN transmitters. The paper derives the maximum averaged power densities allowed and identifies zones where mitigation measures are required.

Antennas↗