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At least 1,207 records · Page 67

Physiological and genetic characterization of plant growth and gravitropism in LED light sources

Among the many problems of growing plants in completely controlled environments, such as those anticipated for the space station and the CELSS program, is the need to provide light that is both adequate for photosynthesis and of proper quality for normal growth and development. NASA scientists and engineers have recently become interested in the possibility of utilizing densely packed, solid state, light emitting diodes (LED's) as a source for this light. Unlike more conventional incandescent or electrical discharge lamps, these sources are highly monochromatic and lack energy in spectral regions thought to be important for normal plant development. In addition, a recent observation by NASA scientist has suggested that infra-red LED's, that are routinely used as photographic safelights for plants grown in darkness, may interact with the ability of plants to detect gravity. In order to establish how plants respond to light from these LED light sources we carried out a series of experiments with known pigment mutants of the model mustard plant, Arabidopsis thaliana, growing in either a gravity field or on a clinostat to simulate a micro-gravity environment. Results indicate that only red light from the 665 nm LED's disrupts the ability of normal wildtype seedlings to detect a gravity stimulus. There was no consistent effect found for the far-red (735 nm) LED's or either of the infrared (880 nm or 935 nm) LED sources but both showed some effect in one or more of the genotypes tested. Of these five members of the phytochrome multigene family in Arabidopsis, only the phytochrome B pigment mutant (hy3) lacked the ability to detect gravity under all conditions. There was no effect of either micro-gravity (clinostat) or the infra-red LED's on the light induced inhibition of hypocotyl elongation. Measurements of the pigment phytochrome in oats also showed no photoconversion by 15 min irradiations with the infra-red LED's. We conclude that phytochrome B is required for the perception of gravity and that only red light is able to disrupt this perception. The infra-red LED's also do not appear to interact with gravity perception in Arabidopsis, but caution should be exercised if infra-red LED's are to be used as photographic safelights for these types of experiments.

Deitzer, Gerald F.↗

Benefits of a Single-Person Spacecraft for Weightless Operations

Historically, less than 20 percent of crew time related to extravehicular activity (EVA) is spent on productive external work.1 A single-person spacecraft with 90 percent efficiency provides productive new capabilities for maintaining the International Space Station (ISS), exploring asteroids, and servicing telescopes or satellites. With suits, going outside to inspect, service or repair a spacecraft is time-consuming, requiring pre-breathe time, donning a fitted space suit, and pumping down an airlock. For ISS, this is between 12.5 and 16 hours for each EVA, not including translation and work-site set up. The work is physically demanding requiring a day of rest between EVAs and often results in suit-induced trauma with frequent injury to astronauts fingers2. For maximum mobility, suits use a low pressure, pure oxygen atmosphere. This represents a fire hazard and requires pre-breathing to reduce the risk of decompression sickness (bends). With virtually no gravity, humans exploring asteroids cannot use legs for walking. The Manned Maneuvering Unit offers a propulsive alternative however it is no longer in NASA s flight inventory. FlexCraft is a single person spacecraft operating at the same cabin atmosphere as its host so there is no risk of the bends and no pre-breathing. This allows rapid, any-time access to space for repeated short or long EVAs by different astronauts. Integrated propulsion eliminates hand-over-hand translation or having another crew member operate the robotic arm. The one-size-fits-all FlexCraft interior eliminates the suit part inventory and crew time required to fit all astronauts. With a shirtsleeve cockpit, conventional displays and controls are used and because the work is not strenuous no rest days are required. Furthermore, there is no need for hand tools because manipulators are equipped with force multiplying end-effectors that can deliver the precise torque for the job.

Griffin, Brand N.↗

Exploration Extravehicular Mobility Unit (xEMU) Pressure Garment System (PGS) Cycle Testing Overview and Results

With the development of NASA’s next generation spacesuit, the hardware life expectations for the new spacesuit required evaluation. The xPGS team designed and performed a test series to assess the new hardware against the life requirements. The EMU on the International Space Station (ISS) today tests the life of the suit and new components by performing isometric individual joint cycle motions with the requirements based on previously performed Extra-Vehicular Activities (EVAs). The xPGS includes new designs for suit components to provide increased mobility for performance of lunar operations, thus the requirements for the life cycle of the suit had to be developed and tested with these new operations in mind. Cycle requirements shifted to cycles of functional tasks in lieu of isometric cycles to better understand the life of the suit. Manned cycle testing of the xPGS was conducted by performing repetitions of a series of tasks that reflect predicted Lunar EVA operations. The test series utilized a gravity offload system to simulate Lunar gravity environment. Cycle testing concluded after 30 test days in October of 2022 with lessons learned that will be critical for future spacesuit design. Specific lessons learned with regards to suit performance and test methodology will be provided.

spacesuit↗

Enhancement of Pool Boiling Heat Transfer and Control of Bubble Motion in Microgravity Using Electric Fields (BCOEL)

The BCOEL project focuses on improving pool boiling heat transfer and bubble control in microgravity by exposing the fluid to electric fields. The electric fields induce a body force that can replace gravity in the low gravity environment, and enhance bubble removal from the heated surface. A better understanding of microgravity effects on boiling with and without electric fields is critical to the proper design of the phase-change-heat-removal equipment for use in spacebased applications. The microgravity experiments will focus on the visualization of bubble formation and shape during boiling. Heat fluxes on the boiling surface will be measured, and, together with the measured driving temperature differences, used to plot boiling curves for different electric field magnitudes. Bubble formation and boiling processes were found to be extremely sensitive to g-jitter. The duration of the experimental run is critical in order to achieve steady state in microgravity experiments. The International Space Station provides conditions suitable for such experiments. The experimental apparatus to be used in the study is described in the paper. The apparatus will be tested in the KC-135 first, and microgravity experiments will be conducted on board of the International Space Station using the Microgravity Science Glovebox as the experimental platform.

Herman, Cila↗

An intelligent robot for helping astronauts

This paper describes the development status of a prototype supervised intelligent robot for space application for purposes of (1) helping the crew of a spacecraft such as the Space Station with various tasks, such as holding objects and retrieving/replacing tools and other objects from/into storage, and (2) for purposes of retrieving detached objects, such as equipment or crew, that have become separated from their spacecraft. In addition to this set of tasks in this low-Earth-orbiting spacecraft environment, it is argued that certain aspects of the technology can be viewed as generic in approach, thereby offering insight into intelligent robots for other tasks and environments. Candidate software architectures and their key technical issues which enable real work in real environments to be accomplished safely and robustly are addressed. Results of computer simulations of grasping floating objects are presented. Also described are characterization results on the usable reduced gravity environment in an aircraft flying parabola (to simulate weightlessness) and results on hardware performance there. These results show it is feasible to use that environment for evaluative testing of dexterous grasping based on real-time vision of freely rotating and translating objects.

Erickson, J. D.↗

Two-Phase Flow in Packed Beds, Filters and Orifices: Summary of Findings of the Packed Bed Reactor Experiment Onboard of the International Space Station

Understanding the hydrodynamics of adiabatic two-phase flows in packed bed reactors enables the optimization of chemical reaction rates and products that crucial in pharmaceuticals and energy production industries. Additionally, this understanding aids in designing more efficient and compact reactors and reducing space and resource requirements. Insights gained from studying and understanding such flows in microgravity contribute to advancements of space technologies and the enhancement of our capabilities for undertaking future long duration safe, and sustainable space exploration missions. The objective of the Packed Bed Reactor Experiment series (PBRE and PBRE-2) was to develop a fundamental understanding of the hydrodynamics of adiabatic two-phase flows through porous media in microgravity. This work provides a description of the PBRE fluid system and presents the highlights of the PBRE and PBRE-2 previously flown with different packings and operated onboard of the International Space Station Microgravity Science Glovebox (ISS-MSG). These highlights include flow patterns and two-phase pressure drop, and the impact of capillary effects on pressure drop at various gas and liquid flow rates. This work also presents preliminary results from the Packed Bed Reactor Experiment-Water Recovery Series (PBRE-WRS) recently flown with filters and orifices, which was also operated on the ISS-MSG. The objective of the experiment is to validate hydrodynamic models at a range of gas and liquid flow rates pertinent to the operation of these test articles. The microgravity environment allows for measurement and observation of aspects of fluid dynamics that are unique when compared to observations made in full or partial gravity environments.

ISS↗

Gas/Liquid Separator Being Developed for Microgravity

The examination and research of how liquids and gases behave in very low gravity will improve our understanding of the behavior of fluids on Earth. The knowledge of multiphase fluid behavior is applicable to many industries on Earth, including the pharmaceutical, biotechnology, chemical, and nuclear industries, just to name a few. In addition, this valuable knowledge applies very well to the engineering and design of microgravity materials processing and of life-support systems for extended space flight. Professors Ashok Sangani of Syracuse University and Donald Koch of Cornell University are principal investigators in the Microgravity Fluid Physics Program, which is managed and sponsored by the NASA Glenn Research Center. Their flight experiment entitled "Microgravity Observations of Bubble Interactions" (MOBI) is planned for operation in the Fluids and Combustion Facility aboard the International Space Station.

Hoffmann, Monica I.↗

Enhancement of Pool Boiling Heat Transfer and Control of Bubble Motion in Microgravity Using Electric Fields - BCOEL

The BCOEL project focuses on improving pool boiling heat transfer and bubble control in microgravity by exposing the fluid to electric fields. The electric fields induce a body force that can replace gravity in the low gravity environment, and enhance bubble removal from thc heated surface. A better understanding of microgravity effects on boiling with and without electric fields is critical to the proper design of the phase-change-heat-removal equipment for use in space-based applications. The microgravity experiments will focus on the visualization of bubble formation and shape during boiling. Heat fluxes on the boiling surface will be measured, and, together with the measured driving temperature differences, used to plot boiling curvcs for different electric field magnitudes. Bubble formation and boiling processes were found to be extremely sensitive to g-jitter. The duration of the experimental run is critical in order to achieve steady state in microgravity experiments. The International Space Station provides conditions suitable for such experiments. The experimental appararus to be used in the study is described in the paper. The apparatus will be tested in the KC-135 first, and microgravity experiments will be conducted on board of the International Space Station using the Microgravity Science Glovebox as the experimental platform.

Herman, Cila↗

Sodium Extraction from Full-Length Fermi-1 Blanket Assembly

Enrico Fermi Nuclear Generating Station is a nuclear power plant on the shore of Lake Erie. On October 5, 1966, Fermi 1, a prototype fast breeder reactor, suffered a partial fuel meltdown (no radioactive material was released). As a result, 34 metric tons of sodium-bonded blanket material from the decommissioned Fermi-1 reactor were shipped to and are currently stored at the Idaho Nation Lab (INL). This material is currently awaiting removal from the state of Idaho by 2035. In its current state, the Fermi-1 material is not suitable for disposal due to the reactive characteristic of its bond sodium. This work demonstrates removal of bond sodium from an entire full-length unirradiated Fermi-1 outer radial blanket assembly using a gravity assisted melt-drain-evaporate (MEDE) treatment process. Quantitative and qualitative analyses were performed to characterize the extent of sodium removal from the blanket material following MEDE treatment.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Space Station Freedom combustion research

Extended operations in microgravity, on board spacecraft like Space Station Freedom, provide both unusual opportunities and unusual challenges for combustion science. On the one hand, eliminating the intrusion of buoyancy provides a valuable new perspective for fundamental studies of combustion phenomena. On the other hand, however, the absence of buoyancy creates new hazards of fires and explosions that must be understood to assure safe manned space activities. These considerations - and the relevance of combustion science to problems of pollutants, energy utilization, waste incineration, power and propulsion systems, and fire and explosion hazards, among others - provide strong motivation for microgravity combustion research. The intrusion of buoyancy is a greater impediment to fundamental combustion studies than to most other areas of science. Combustion intrinsically heats gases with the resulting buoyant motion at normal gravity either preventing or vastly complicating measurements. Perversely, this limitation is most evident for fundamental laboratory experiments; few practical combustion phenomena are significantly affected by buoyancy. Thus, we have never observed the most fundamental combustion phenomena - laminar premixed and diffusion flames, heterogeneous flames of particles and surfaces, low-speed turbulent flames, etc. - without substantial buoyant disturbances. This precludes rational merging of theory, where buoyancy is of little interest, and experiments, that always are contaminated by buoyancy, which is the traditional path for developing most areas of science. The current microgravity combustion program seeks to rectify this deficiency using both ground-based and space-based facilities, with experiments involving space-based facilities including: laminar premixed flames, soot processes in laminar jet diffusion flames, structure of laminar and turbulent jet diffusion flames, solid surface combustion, one-dimensional smoldering, ignition and flame spread of liquids, drop combustion, and quenching of panicle-air flames. Unfortunately, the same features that make microgravity attractive for fundamental combustion experiments, introduce new fire and explosion hazards that have no counterpart on earth. For example, microgravity can cause broader flammability limits, novel regimes of flame spread, enhanced effects of flame radiation, slower fire detector response, and enhanced combustion upon injecting fire extinguishing agents, among others. On the other hand, spacecraft provide an opportunity to use 'fire-safe' atmospheres due to their controlled environment. Investigation of these problems is just beginning, with specific fire safety experiments supplementing the space based fundamental experiments listed earlier; thus, much remains to be done to develop an adequate technology base for fire and explosion safety considerations for spacecraft.

Faeth, G. M.↗

Quick-Connect Windowed Non-Stick Penetrator Tips for Rapid Sampling

Standard penetrator sampling systems were designed in order to allow for sampling via penetrators to produce a full set of sample acquisitions including volatile liquids, fine powders, and solid fragments. A gravity harpoon sampler has been designed with a removable tip and a quick coupling. The separation allows for sample handling and eliminates sample cross-contamination. Also, this design allows for multiple use of the penetrator body, which is the largest and heaviest part of the penetrator, while allowing for multiple changes of the light-mass, penetrator tip to avoid sample cross-contamination. The penetrator tip design has been improved by adding a spring trap to retain the sample, as well as a means for connecting to a quick coupling. Quick connect tips have been demonstrated in a sample handling carousel. The penetrator was released and rewound and the tips were released into a circular platter for rotation into instrument stations. The pyro-harpoon sampler was fabricated and tested with a NASA Standard Initiator (NSI) pyrotechnic charge. Initial tests collected cryogenic ice, but removal of the small pyro-harpoon from the ice was difficult. A brass metal sheath was then fitted over the harpoon tip, and removal from the ice was greatly alleviated by leaving the sheath in the ice. Quartz windows in the tips allow direct optical and spectral imaging and gas chromatography-mass spectrometer (GCMS) pyrolysis, and were found to survive impact. All systems were successfully tested by dropping into sand and into cryogenic ice.

Sherritt, Stewart↗

Deformation and Flexibility Equations for Idealized ARIS Umbilicals, Under Planar End-Loading Conditions

The International Space Station (ISS) relies on the Active Rack Isolation System (ARIS) as the central component of an integrated, station-wide strategy to isolate microgravity space-science experiments. ARIS uses electromechanical actuators to isolate an International Standard Payload Rack (ISPR) from disturbances due to the motion of the ISS. Disturbances to microgravity experiments on ARIS-isolated racks are primarily transmitted via the ARTS power and vacuum umbilicals. Recent experimental tests indicate that these umbilicals resonate at frequencies outside the ARIS controller's bandwidth, at levels of potential concern for certain microgravity experiments. Reduction in the umbilical resonant frequencies could help to address this issue. This report develops equations for the in-plane deflections and flexibilities of an idealized umbilical (thin, flexible, cantilever beam) under end-point, in-plane loading (inclined-force and moment). The effect of gravity is neglected due to the on-orbit application. The analysis assumes an initially straight, cantilevered umbilical with uniform cross-section, which undergoes large deflections with no plastic deformation, such that the umbilical terminus remains in a single quadrant and the umbilical slope changes monotonically. The analysis is applicable to the ARIS power and vacuum umbilicals, under the indicated assumptions.

Hampton, R. David↗

Deformation and Flexibility Equations for Idealized ARIS Umbilicals, Under Planar End-Loading Conditions

The International Space Station (ISS) relies on the Active Rack Isolation System (ARIS) as the central component of an integrated, station-wide strategy to isolate microgravity space-science experiments. ARIS uses electromechanical actuators to isolate an International Standard Payload Rack (ISPR) from disturbances due to the motion of the ISS. Disturbances to microgravity experiments on ARIS-isolated racks are primarily transmitted via the ARIS power and vacuum umbilicals. Recent experimental tests indicate that these umbilicals resonate at frequencies outside the ARIS controller's bandwidth. at levels of potential concern for certain microgravity experiments. Reduction in the umbilical resonant frequencies could help to address this issue. This paper develops equations for the in-plane deflections and flexibilities of an idealized umbilical (thin, flexible, cantilever beam) under end-point, in-plane loading (inclined-force and moment). The effect of gravity is neglected due to the on:orbit application. The analysis assumes an initially straight. cantilevered umbilical with uniform cross-section. which undergoes large deflections with no plastic deformation, such that the umbilical terminus remains in a single quadrant and the umbilical slope changes monotonically. The analysis is applicable to the ARIS power and vacuum umbilicals. under the indicated assumptions.

Hampton, R. David↗

A Thermal Review of the Sample Cartridge Assembly (SCA) Gravitational Effect of Distortion in Sintering (GEDS) Experiment Flight Processing

NASA’s Sample Cartridge Assembly (SCA) first flight experiment, Gravitational Effect of Distortion in Sintering (GEDS), was processed on the International Space Station (ISS) between 2019 and 2020. The SCA was heated in the European Space Agency’s (ESA) Low Gradient Furnace (LGF) that is housed inside the Material Science Research Rack (MSRR) located in the U.S. Laboratory Module. This summary will give a review of the design and flight experiment development for the GEDS SCA. It describes flight processing and the role of thermal engineering support. Lessons learned and future Principle Investigators (PI) will be discussed.

Micro-gravity Science↗

X-48B Flight Test Progress Overview

The results of a series of 39 flight tests of the X-48B Low Speed Vehicle (LSV) performed at the NASA Dryden Flight Research Center from July 2007 through December 2008 are reported here. The goal of these tests is to evaluate the aerodynamic and controls and dynamics performance of the subscale LSV aircraft, eventually leading to the development of a control system for a full-scale vehicle. The X-48B LSV is an 8.5%-scale aircraft of a potential, full-scale Blended Wing Body (BWB) type aircraft and is flown remotely from a ground control station using a computerized flight control system located onboard the aircraft. The flight tests were the first two phases of a planned three-phase research program aimed at ascertaining the flying characteristics of this type of aircraft. The two test phases reported here are: 1) envelope expansion, during which the basic flying characteristics of the airplane were examined, and 2) parameter identification, stalls, and engine-out testing, during which further information on the aircraft performance was obtained and the airplane was tested to the limits of controlled flight. The third phase, departure limiter assaults, has yet to be performed. Flight tests in two different wing leading edge configurations (slats extended and slats retracted) as well as three weight and three center of gravity positions were conducted during each phase. Data gathered in the test program included measured airplane performance parameters such as speed, acceleration, and control surface deflections along with qualitative flying evaluations obtained from pilot and crew observations. Flight tests performed to-date indicate the aircraft exhibits good handling qualities and performance, consistent with pre-flight simulations.

Risch, Timoth K.↗

Study of the Correlation between the Performances of Lunar Vehicle Wheels Predicted by the Nepean Wheeled Vehicle Performance Model and Test Data

This paper describes the results of a study of the correlation between the performances of wheels for lunar vehicles predicted using the Nepean wheeled vehicle performance model (NWVPM), developed under the auspices of Vehicle Systems Development Corporation, Ottawa, Canada, and the corresponding test data presented in Performance evaluation of wheels for lunar vehicles , Technical Report M-70-2, prepared for George C. Marshall Space Flight Center, National Aeronautics and Space Administration (NASA), USA, by the US Army Engineer Waterways Experiment Station (WES). The NWVPM was originally developed for design and performance evaluation of terrestrial off-road wheeled vehicles. The purpose of this study is to assess the potential of the NWVPM for evaluating wheel candidates for the new generation of extra-terrestrial vehicles. Two versions of a wire-mesh wheel and a hoop-spring wheel, which were considered as candidates for lunar roving vehicles for the NASA Apollo program in the late 1960s, together with a pneumatic wheel were examined in this study. The tractive performances of these wheels and of a 464 test vehicle with the pneumatic wheels on air-dry sand were predicted using the NWVPM and compared with the corresponding test data obtained under Earth s gravity and previously documented in the above-named report. While test data on wheel or vehicle performances obtained under Earth s gravity may not necessarily be representative of those on extra-terrestrial bodies, because of the differences in gravity and in environmental conditions, such as atmospheric pressure, it is still a valid approach to use test data obtained under Earth s gravity to evaluate the predictive capability of the NWVPM and its potential applications to predicting wheel or wheeled rover performances on extra-terrestrial bodies. Results of this study show that, using the ratio (P20/W) of the drawbar pull to normal load at 20 per cent slip as a performance indicator, there is a reasonable correlation between the predictions and experimental data. This indicates that the NWVPM has the potential as an engineering tool for evaluating wheel candidates for a future generation of extra-terrestrial vehicles, provided that appropriate input data are available.

Wong, J. Y.↗

Response of Staphylococcus Aureus to a Spaceflight Analogue

The decreased gravity of the spaceflight environment creates quiescent, low fluid shear conditions. This environment can impart considerable effects on the physiology of microorganisms as well as their interactions with potential hosts. Using the rotating wall vessel (RWV), as a spaceflight analogue, the consequence of low fluid shear culture on microbial pathogenesis has provided a better understanding of the risks to the astronaut crew from infectious microorganisms. While the outcome of low fluid shear culture has been investigated for several bacterial pathogens, little has been done to understand how this environmental factor affects Staphylococcus aureus. S. aureus is an opportunistic human pathogen which presents a high level of infection risk to the crew, as it has been isolated from both the space shuttle and International Space Station. Given that approximately forty percent of the population are carriers of the bacteria, eradication of this organism from in flight environments is impractical. These reasons have lead to us to assess the response of S. aureus to a reduced fluid shear environment. Culture in the RWV demonstrated that S. aureus grown under the low-shear condition had lower cell concentrations after 10 hours when compared to the control culture. Furthermore, the low-shear cultured bacteria displayed a reduction in carotenoid production, pigments responsible for their yellow/gold coloration. When exposed to various environmental stressors, post low-shear culture, a decrease in the ability to survive oxidative assault was observed compared to control cultures. The low fluid shear environment also resulted in a decrease in hemolysin secretion, a staphylococcal toxin responsible for red blood cell lysis. When challenged by the immune components present in human whole blood, low-shear cultured S. aureus demonstrated significantly reduced survival rates as compared to the control culture. Assays to determine the duration of these alterations demonstrated that the low-shear response could be lost in as few as 2.5 hours. These changes in phenotypic properties prompted investigation into variations occurring at the genetic level. Microarray analysis of low-shear cultured S. aureus revealed the differential regulation of genes involved in metabolism, stress response, and phosphate transfer. Additional genetic analysis with quantitative real-time PCR revealed alterations in the expression of Hfq, the conserved RNA chaperone protein involved in global gene regulation. Hfq has been connected to the regulation of a spaceflight microgravity response in S. typhimurium. These findings in S. aureus suggest an evolutionary conserved response to spaceflight conditions among structurally-diverse microorganisms. Furthermore, the reduction in pigmentation, hemolysin secretion, and survival against oxidative stress and immunologically active whole blood demonstrate an overall decrease in the virulence factors of S. aureus in response to spaceflight-like conditions.

Castro, S. L.↗

Countermeasures for Mitigation of Sensorimotor Decrements Following Head-Down Tilt Bed Rest

BACKGROUND Astronauts experience postflight disturbances in postural and locomotor control due to sensorimotor adaptations during spaceflight. These alterations may have adverse consequences if a rapid egress is required after landing. Although exercise is partially effective for mitigating cardiovascular and muscular deconditioning, additional countermeasures are needed to further preserve sensorimotor function. Proprioception training and electrical muscle stimulation (EMS) are two promising in-flight countermeasures. Since prolonged head down tilt bed rest (HDTBR) is a spaceflight analog for body unloading and causes postural and locomotor control decrements that parallel those observed after spaceflight, it can be used to facilitate the development of these countermeasures. METHODS This study will determine the effects of proprioception training and EMS on functional task performance and sensorimotor function following 60 days of 6° HDTBR. Subjects will be randomly assigned to one of four groups: 1) an EMS arm, 2) a proprioception training arm, 3) an exercise plus proprioceptive training arm, and 4) a control arm. The EMS countermeasure will include daily bilateral stimulation of selected bilateral lower extremity muscles (30 minutes per session). Proprioception training will be performed three days per week (25 minutes per session) consisting of body-loaded postural tasks in the horizontal position on an air bearing sled. Exercise training will mimic current protocols used on the International Space Station, but treadmill aerobic exercise will be replaced with additional cycling aerobic exercise. Primary outcome measures will include pre- and post- HDTBR functional tests that require high demand for dynamic control of postural stability. Secondary measures will be used to explore key physiological changes that underlie countermeasure benefits. All HDTBR and data collection activities will be completed by the German Aerospace Center (DLR) at the :envihab facility. Given the constrained samples size, a Bayesian modelling approach will be used to quantify the probability that there is an effect of a given magnitude. HARDWARE AND PROTOCOL DEVELOPMENT Final hardware modifications and protocol developments were completed in preparation for Campaign 1, which began in September 2024. These included shipment, setup, and operator training for the transportable gravity bed, horizontal squat device, foam obstacle course, EMS devices, leg dexterity system, foot sole skin sensitivity system, and Radiofrequency Echographic Multi Spectrometry (REMS) ultrasound device. In addition, specialized protocols were developed for data collection using DLR’s equipment, including muscle morphology magnetic resonance imaging (MRI), optical coherence tomography, venous blood flow MRI and ultrasound, muscle ultrasound and impedance, and skin blood flow ultrasound. We will present early data from the first campaign, which concluded in November, 2024. These will be compared with previous data from the recent 30-day HDTBR campaigns (Spaceflight associated neuro-ocular syndrome countermeasures (SANS-CM)) conducted at DLR. RELEVANCE The deliverable from this project will be proof-of-concept sensorimotor countermeasure designs for functional task performance with full assessment of efficacy in a spaceflight analog. If one or more countermeasures are effective, they will be translated for validation with the suite of operationally implemented in-flight countermeasures.

T R Macaulay↗