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At least 1,207 records · Page 67

Perfection of Apoferritin Crystals: An Advanced X-Ray Imaging and Diffraction Study

Ferritin is a well-known iron-storage protein, and is a spherical shell that consists of 24 identical subunits packed in a 432 symmetry. The typically large protein size and its distinction from lysozyme as to chemical and physical characteristics make ferritin an attractive model protein for crystal growth and perfection investigation-as an alternative to the most widely studied lysozyme. In this contribution, the latest results obtained from coherence-based x-ray diffraction imaging and diffraction experiments will be presented on octahedral apoferritin (a demetalized form of ferritin) crystals grown from various growth conditions. Crystal specimens, which have the measured rocking-curve widths varying from a few arcseconds to several tens arcseconds (or more), are comparatively examined by intrinsically highly sensitive mapping of lattice perfection and defects. The richness of the observed defects and growth features offers insight into perfection and growth of protein crystals. Beautiful interference fringe patterns formed in diffraction images and fine oscillation structure of rocking curves observed will be discussed for understanding of physical origins and the underlying impact.

Hu, Z. W.↗

Infrared and Passive Microwave Radiometric Sea Surface Temperatures and Their Relationships to Atmospheric Forcing

The current generation of infrared (IR) and passive microwave (MW) satellite sensors provides highly complementary information for monitoring sea surface temperature (SST). On the one hand, infrared sensors provide high resolution and high accuracy but are obscured by clouds. Microwave sensors on the other hand, provide coverage through non-precipitating clouds but have coarser resolution and generally poorer accuracy. Assuming that the satellite SST measurements do not have spatially variable biases, they can be blended combining the merits of both SST products. These factors have motivated recent work in blending the MW and IR data in an attempt to produce high-accuracy SST products with improved coverage in regions with persistent clouds. The primary sources of retrieval uncertainty are, however, different for the two sensors. The main uncertainty in the MW retrievals lies in the effects of wind-induced surface roughness and foam on emissivity, whereas the IR retrievals are more sensitive to the atmospheric water vapor and aerosol content. Average nighttime differences between the products for the month periods of January 1999 and June 2000 are shown. These maps show complex spatial and temporal differences as indicated by the strong spatially coherent features in the product differences and the changes between seasons. Clearly such differences need to be understood and accounted for if the products are to be combined. The overall goals of this project are threefold: (1) To understand the sources of uncertainty in the IR and MW SST retrievals and to characterize the errors affecting the two types of retrieval as a fiction of atmospheric forcing; (2) To demonstrate how representative the temperature difference between the two satellite products is of Delta T; (3) To apply bias adjustments and to device a comprehensive treatment of the behavior of the temperature difference across the oceanic skin layer to determine the best method for blending thermal infrared and passive microwave measurements of SSTs.

Castro, Sandra L.↗

Geologic Map of the Snegurochka Planitia Quadrangle (V-1): Implications for the Volcanic History of the North Polar Region of Venus

Geologic mapping of Snegurochka Planitia (V-1) reveals a complex stratigraphy of tectonic and volcanic features that can provide insight into the geologic history of Venus and Archean Earth [1,2], including 1) episodes of both localized crustal uplift and mantle downwelling, 2) shifts from local to regional volcanic activity, and 3) a shift back to local volcanic activity. We present our interpretations of the volcanic history of the region surrounding the north pole of Venus and explore how analysis of new data support our interpretations

Hurwitz, D. M.↗

Simulation of Galactic Cosmic Rays and Dose-Rate Effects in RITRACKS

The NASA Space Radiation Laboratory (NSRL) facility has been used successfully for many years to generate ion beams for radiation research experiments by NASA investigators. Recently, modifications were made to the beam lines to allow rapid switching between different types of ions and energies, with the aim to simulate the Galactic Cosmic Rays (GCR) environment. As this will be a focus of space radiation research for upcoming years, the stochastic radiation track structure code RITRACKS (Relativistic Ion Tracks) was modified to simulate beams of various ion types and energies during time intervals specified by the user at the microscopic and nanoscopic scales. For example, particle distributions of a mixed 344.1-MeV protons (18.04 cGy) and 950-MeV/n iron (5.64 cGy) beam behind a 20 g/cm(exp 2) aluminum followed by a 10 g/cm(exp 2) polyethylene shield as calculated by the code GEANT4 were used as an input field in RITRACKS. Similarly, modifications were also made to simulate a realistic radiation environment in a spacecraft exposed to GCR by sampling the ion types and energies from particle spectra pre-calculated by the code HZETRN. The newly implemented features allows RITRACKS to generate time-dependent differential and cumulative 3D dose voxel maps. These new capabilities of RITRACKS will be used to investigate dose-rate effects and synergistic interactions of various types of radiations for many end points at the microscopic and nanoscopic scales such as DNA damage and chromosome aberrations.

Plante, Ianik↗

A Novel Surface Feature Navigation Algorithm Using Ray Tracing

We demonstrate a novel single-bounce ray tracing approach to landmark identification for surface feature-based relative navigation. A priori knowledge of the camera pose and known topographic maps for each landmark are used to render the potentially visible landmarks via ray tracing into the image frame. These templates are registered with a search region around the predicted location for each landmark in the image, to locate its observed center. This procedure is applied to images from the OSIRIS-REx Orbital A and Orbital B mission phases, and the results are compared with those obtained via previous landmark identification methods.

Gnam, Chris↗

Numerous Wildfire Smoke Plumes in Northwest Ontario, Canada

The height at which smoke is lofted into the atmosphere affects how long it will stay aloft, how far it will travel, and how much of an impact it will have on air quality downwind, and regional climate. Parallax, the change in apparent plume position relative to the surface, as observed by NASA's Multi0angle Imaging Spectroradiometer (MISR) instrument, makes it possible to map the height of smoke, dust and volcanic plumes near-source, where plume features are visible in the multi-angle views.

wildfire smoke↗

Investigating Photogrammetric Accuracy of a Lunar-lander-induced Crater Measurement System

Laboratory measurements have been made to validate the performance of the Stereo CAmeras for Lunar Plume-Surface Studies (SCALPSS) stereo photogrammetry systems which will be flying to the moon on two of NASA’s upcoming Commercial Lunar Payload Services (CLPS) missions. Until recently, the system’s accuracy had only been studied using idealized geometric shapes as measurement targets. A realistic crater model of representative scale and an idealized ‘staircase’ target have been used to compare measurement accuracy of ideal versus lunar-like objects, with the commercial V-STARS® system being used to provide the known reference values for comparison. In a parametric study, altitude, lens focal length, and camera separation are varied to assess each parameter’s impact on photogrammetric accuracy in relation to the scaling law prediction developed previously. The SCALPSS 1.0 and 1.1 configurations have been validated on the crater model within acceptable accuracy for the missions, performing significantly better than the scaling law prediction in some cases. A semi-automated post-processing routine was developed in MATLAB® and proved successful for the cross-correlation of features between two stereo images. For some cases of extreme convergence angles between a camera pair, manual feature detection and matching was required. By using this manual process, the crater depth map was reconstructed but with worse accuracy than the idealized staircase measurements; refinements to the processing algorithm are expected to improve future results. Also examined in this work is the impact of illumination environments, both natural (e.g., Sun angles) and artificial (diffuse or structured illumination sources), on the camera system’s ability to measure the erosion of the lunar terrain.

Plume-surface interaction↗

Investigating Photogrammetric Accuracy of a Lunar-lander-induced Crater Measurement System

Laboratory measurements have been made to validate the performance of the Stereo CAmeras for Lunar Plume-Surface Studies (SCALPSS) stereo photogrammetry systems which will be flying to the moon on two of NASA’s upcoming Commercial Lunar Payload Services (CLPS) missions. Until recently, the system’s accuracy had only been studied using idealized geometric shapes as measurement targets. A realistic crater model of representative scale and an idealized ‘staircase’ target have been used to compare measurement accuracy of ideal versus lunar-like objects, with the commercial V-STARS® system being used to provide the known reference values for comparison. In a parametric study, altitude, lens focal length, and camera separation are varied to assess each parameter’s impact on photogrammetric accuracy in relation to the scaling law prediction developed previously. The SCALPSS 1.0 and 1.1 configurations have been validated on the crater model within acceptable accuracy for the missions, performing significantly better than the scaling law prediction in some cases. A semi-automated post-processing routine was developed in MATLAB® and proved successful for the cross-correlation of features between two stereo images. For some cases of extreme convergence angles between a camera pair, manual feature detection and matching was required. By using this manual process, the crater depth map was reconstructed but with worse accuracy than the idealized staircase measurements; refinements to the processing algorithm are expected to improve future results. Also examined in this work is the impact of illumination environments, both natural (e.g., Sun angles) and artificial (diffuse or structured illumination sources), on the camera system’s ability to measure the erosion of the lunar terrain.

Plume-surface interaction↗

Geologic evaluation and applications of ERTS-1 imagery over Georgia

Satellite imagery and other remote sensing tools and techniques have provided a powerful tool to assist geologic research; significantly increased the mapping efficiency of field geologists; shown new lineaments associated with known shear and fault zones; delineated new structural features; provided a tool to reevaluate tectonic history; helped to locate potential ground-water sources and areas of aquifer recharge; defined areas of geologic hazards; shown areas of heavy siltation in major reservoirs; and, by close interval repetition, aided in monitoring surface mine reclamation activities and the environmental protection of the intricate marshland system. The Georgia Geological Survey has been engaged in regional mapping for the new state geologic map. ERTS-1 images enlarged to compatible mapping scales have increased field geologic mapping efficiency by at least 25%. There are a number of areas where data from ERTS-1 imagery has allowed a notably higher level of precision than has been available with any amount of field work on the ground.

Pickering, S. M.↗

CCSDS Advanced Orbiting Systems Virtual Channel Access Service for QoS MACHETE Model

To support various communications requirements imposed by different missions, interplanetary communication protocols need to be designed, validated, and evaluated carefully. Multimission Advanced Communications Hybrid Environment for Test and Evaluation (MACHETE), described in "Simulator of Space Communication Networks" (NPO-41373), NASA Tech Briefs, Vol. 29, No. 8 (August 2005), p. 44, combines various tools for simulation and performance analysis of space networks. The MACHETE environment supports orbital analysis, link budget analysis, communications network simulations, and hardware-in-the-loop testing. By building abstract behavioral models of network protocols, one can validate performance after identifying the appropriate metrics of interest. The innovators have extended the MACHETE model library to include a generic link-layer Virtual Channel (VC) model supporting quality-of-service (QoS) controls based on IP streams. The main purpose of this generic Virtual Channel model addition was to interface fine-grain flow-based QoS (quality of service) between the network and MAC layers of the QualNet simulator, a commercial component of MACHETE. This software model adds the capability of mapping IP streams, based on header fields, to virtual channel numbers, allowing extended QoS handling at link layer. This feature further refines the QoS v existing at the network layer. QoS at the network layer (e.g. diffserv) supports few QoS classes, so data from one class will be aggregated together; differentiating between flows internal to a class/priority is not supported. By adding QoS classification capability between network and MAC layers through VC, one maps multiple VCs onto the same physical link. Users then specify different VC weights, and different queuing and scheduling policies at the link layer. This VC model supports system performance analysis of various virtual channel link-layer QoS queuing schemes independent of the network-layer QoS systems.

Jennings, Esther H.↗

Characteristic structures of the highland boundary on Mars: Evidence against a single mega-impact event?

It has been suggested that an early mega-impact event might explain the fumdamental crustal dichotomy on Mars. Detailed morphological mapping of the structures which characterize the boundary between the cratered highlands and northern plains does not support this idea: the distribution of these features along and especially away from the boundary is more consistent with a larger number of smaller but overlapping impacts. A data base was assembled for the study of the highland boundary through quantitative mapping (including crater counts) based on the 1:2 M controlled photomosaics. Whole and partial craters larger than 10 km in diameter, knobby terrain, detached plateaus and intervening plains-forming units have been identified wherever they occur on Mars between +65 and -45 deg latitude. There is evidence for occurrences of possible old cratered terrain (or its remanents) at high latitudes well north of the current or likely former location of the proposed Borealis Basin rim which are difficult to reconcile with an impact structure of the proposed size and location.

Semeniuk, Ann Marie↗

Subcellular Feature-Based Classification of α and β Cells Using Soft X-ray Tomography

The dysfunction of α and β cells in pancreatic islets can lead to diabetes. Many questions remain on the subcellular organization of islet cells during the progression of disease. Existing three-dimensional cellular mapping approaches face challenges such as time-intensive sample sectioning and subjective cellular identification. To address these challenges, we have developed a subcellular feature-based classification approach, which allows us to identify α and β cells and quantify their subcellular structural characteristics using soft X-ray tomography (SXT). We observed significant differences in whole-cell morphological and organelle statistics between the two cell types. Additionally, we characterize subtle biophysical differences between individual insulin and glucagon vesicles by analyzing vesicle size and molecular density distributions, which were not previously possible using other methods. These sub-vesicular parameters enable us to predict cell types systematically using supervised machine learning. We also visualize distinct vesicle and cell subtypes using Uniform Manifold Approximation and Projection (UMAP) embeddings, which provides us with an innovative approach to explore structural heterogeneity in islet cells. This methodology presents an innovative approach for tracking biologically meaningful heterogeneity in cells that can be applied to any cellular system.

3D cell mapping↗

Development of a numerical procedure to map a general 3-d body onto a near-circle

Conformal mapping is a classical technique utilized for solving problems in aerodynamics and hydrodynamics. Conformal mapping is utilized in the construction of grids around airfoils, engine inlets and other aircraft configurations. These shapes are transformed onto a near-circle image for which the equations of fluid motion are discretized on the mapped plane and solved numerically by utilizing the appropriate techniques. In comparison to other grid-generation techniques such as algerbraic or differential type, conformal mapping offers an analytical and accurate form even if the grid deformation is large. One of the most appealing features is that the grid can be constrained to remain orthogonal to the body after the transformation. Hence, the grid is suitable for analyzing the supersonic flow past a blunt object. The associated shock as a coordinate surface adjusts its position in the course of computation until convergence is reached. The present work applied conformal mapping to 3-D bodies with no axis of symmetry such as the Aerobraking Flight Experiment (AFE) vehicle, transforming the AFE shape onto a near-circle image. A numerical procedure and code are used to generate grids around the AFE body.

Hommel, M. J.↗

Evidence for Buried "Pre-Noachian" Crust Pre-Dating the Oldest Observed Surface Units on Mars

Even though the Early Noachian (EN) used in geologic mapping is undefined at the early end, it is often assumed in absolute chronologies to extend back to 4.6 BYA. We explored this assumption by searching for evidence of buried impact basins, in the largest occurrences of Early Noachian terrain. The hypothesis is that if such basins exist, they indicate crust which must predate the surface units mapped as the oldest on Mars, and those units must then be less than 4.6 BY old. Alternatively, if no such buried features are seen, then the surface units may represent crust of the same age below, which could in principle be as old as Mars. Here we show the former alternative is true. There must be crust older than the oldest mapped surface units. We also show that a number of Noachian terrains on Mars appear to have a common total (visible + buried) crater retention age. This might be either the age of the original (planet-wide?) crust of Mars, or may indicate crater saturation.

Frey, H. V.↗

Quantifying Errors in TRMM-Based Multi-Sensor QPE Products Over Land in Preparation for GPM

Determining uncertainties in satellite-based multi-sensor quantitative precipitation estimates over land of fundamental importance to both data producers and hydro climatological applications. ,Evaluating TRMM-era products also lays the groundwork and sets the direction for algorithm and applications development for future missions including GPM. QPE uncertainties result mostly from the interplay of systematic errors and random errors. In this work, we will synthesize our recent results quantifying the error characteristics of satellite-based precipitation estimates. Both systematic errors and total uncertainties have been analyzed for six different TRMM-era precipitation products (3B42, 3B42RT, CMORPH, PERSIANN, NRL and GSMap). For systematic errors, we devised an error decomposition scheme to separate errors in precipitation estimates into three independent components, hit biases, missed precipitation and false precipitation. This decomposition scheme reveals hydroclimatologically-relevant error features and provides a better link to the error sources than conventional analysis, because in the latter these error components tend to cancel one another when aggregated or averaged in space or time. For the random errors, we calculated the measurement spread from the ensemble of these six quasi-independent products, and thus produced a global map of measurement uncertainties. The map yields a global view of the error characteristics and their regional and seasonal variations, reveals many undocumented error features over areas with no validation data available, and provides better guidance to global assimilation of satellite-based precipitation data. Insights gained from these results and how they could help with GPM will be highlighted.

Peters-Lidard, Christa D.↗

Unraveling local structures of Salt-in-Water and Water-in-Salt electrolytes via ab initio molecular dynamics

Water-in-salt electrolytes (WiSE) are attractive for electrochemical energy storage applications owing to their wide electrochemical stability windows and inherent safety. The high concentration of salts is widely known to suppress the decomposition of water, which otherwise limits the stability of conventional aqueous salt-in-water electrolytes (SiWE). Nevertheless, the microstructural features of WiSE that lead to this enhanced stability have not yet been fully elucidated. In this work, ab initio molecular dynamics simulations were performed to study the energetic, structural, and spectroscopic properties of LiTFSI SiWE and WiSE solutions. A detailed mapping of water-water and water-anion hydrogen bonding and cation–anion electrostatic interactions is reported for both electrolytes. Structural features are presented in terms of both radial and spatial distribution functions. Analysis of IR and vibrational power spectra reveal key differences in the intermolecular interactions in SiWE and WiSE that arise from modified solvation shell structures. Finally, the results obtained herein reveal the most important structural and spectroscopic differences between the electrolytes in normal and superconcentrated concentrations.

36 MATERIALS SCIENCE↗

Multimodal, microspectroscopic speciation of legacy phosphorus in two US mid-Atlantic agricultural soils

To understand phosphorus (P) mobility in agricultural soils and its potential environmental risk, it is essential to directly measure solid phase P speciation. Often, bulk P K-edge X-ray absorption near edge structure (XANES) spectroscopy followed by linear combination fitting (LCF) is utilized to determine the solid P phases in soil. However, this method may limit results to only a few major phases. Additionally, XANES spectra for different P species may have very similar features, leading to an over- or underestimate of their contribution to LCF. Here, an improved P speciation by pairing multimodal microbeam-X-ray fluorescence (µ-XRF) mapping coupled with µ-XANES (microbeam-X-ray absorption near edge structure) analysis to directly speciate major and minor P phases on the micron scale is provided. We combined maps of both tender (P, sulfur, aluminum, and silicon) and hard energy (calcium, iron [Fe], and manganese) elements to evaluate the elemental co-locations with P. To better account for uncertainty assigning XANES peaks to individual compounds, a more quantitative fingerprinting by “spectral feature analysis” was completed. With this analysis, an R-factor is reported for the fit. These results were compared to traditional LCF. Pre-edge fitting results revealed the presence of a two-component pre-edge feature for phosphate adsorbed to ferrihydrite. Additionally, phytate co-precipitated with ferrihydrite (Phytate-Fe-Cop) had a pre-edge feature, indicating direct association with Fe. Lastly, a unique P species associated with manganese oxide was identified in the soil via multimodal mapping and µ-XANES. These results allow for better prediction of P dissolution and mobility.

36 MATERIALS SCIENCE↗

Geological Map of the Fredegonade (V-57) Quadrangle, Venus: Status Report

The Fredegonde quadrangle (V-57; 50-75degS, 60-120degE, Fig. 1) corresponds to the northeastern edge of Lada Terra and covers a broad area of the topographic province of midlands (0-2 km above MPR [1,2]). This province is most abundant on Venus and displays a wide variety of units and structures [3-11]. The sequence of events that formed the characteristic features of the midlands is crucially important in understanding of the timing and modes of evolution of this topographic province. Topographically, the Fredegonde quadrangle is within a transition zone between the elevated portion of Lada Terra to the west (Quetzalpetlatl-Boala Coronae rise, approx.3.5 km) and the lowland of Aino Planitia to the north and northeast (approx.-0.5 km). This transition is one of the key features of the V-57 quadrangle. In this respect the quadrangle resembles the region of V-4 quadrangle [12] that shows transition between the midlands and the lowlands of Atalanta Planitia. One of the main goals of our mapping within the V-57 quadrangle is comparison of this region with the other transitional topographic zones such as quadrangles V-4 and V-3 [13]. The most prominent features in the V-57 quadrangle are linear deformational zones of grooves and large coronae. The zones characterize the central and NW portions of the map area and represent broad (up to 100s of km wide) ridges that are 100s of m high. Morphologically and topographically, these zones are almost identical to the groove belt/corona complexes at the western edge of Atalanta Planitia [12]. Within the Fredegonde area, however, the zones are oriented at high angles to the general trend of elongated Aino Planitia, whereas within the V-4 quadrangle they are parallel to the edge of Atalanta Planitia. Relatively small (100s of km across, 100s of m deep) equidimensional basins occur between the corona-groove-chains in the area of V-57 quadrangle. These basins are similar to those that populate the area of the V-3 quadrangle [13]. Broad regional plains cover the surface of the basins in both regions. In contrast to Fredegonde, the area of the V-3 quadrangle displays a greater diversity of units and features [13]. Here we describe units that make up the surface within the V-57 quadrangle and present a summary of our geological map that shows the areal distribution of the major groups of units.

Ivanov, M. A.↗