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At least 1,207 records · Page 67

Optimizing a Small RNAseq Analysis Pipeline for NASA GeneLab Using Open-Source Tools and Libraries

Small RNA sequencing (small RNAseq) is a powerful tool for studying the regulation of gene expression in various organisms. Small RNAseq has been leveraged in space biology research to study how expression of small RNAs, e.g. micro RNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs), change upon exposure to the space environment. NASA GeneLab currently hosts small RNAseq raw data derived from space-relevant experiments on the Open Science Data Repository (OSDR). To maximize the accessibility of these data to the scientific community, in addition to hosting raw data, which is only interpretable by bioinformaticians, GeneLab plans to process all small RNAseq datasets and make those processed data available to the scientific community via the OSDR. In this study, we present the development of the GeneLab standardized pipeline for processing small RNAseq datasets. Using human, plant, and synthetic small RNAseq datasets, we interrogate various open-source software and publicly available databases to evaluate their accuracy and reproducibility in each step of the pipeline. For quality control and adapter detection and trimming, we evaluated TrimGalore!, FASTX, SeqKit, and DNApi methods to optimize alignment to reference genomes. We compared BWA, Bowtie, and Bowtie2 to determine the optimal alignment tool. For each alignment tool we also assessed various reference databases, including Ensembl reference genomes and different types of small RNA reference databases, including genome, hairpin, and miRNA references from the miRbase and MirGeneDB databases. To quantify the aligned data, we compared SAMtools, HTSeq, and RSEM for counting alignment events from each alignment tool used. Finally, we evaluated various tools, including DESeq2 and EdgeR, for data normalization and subsequent differential expression analysis. We will present the results from our comparative analyses for each pipeline step and propose a consensus pipeline for processing small RNAseq data derived from various organisms exposed to the space environment.

SmallRNAseq, NASA GeneLab, quality control, adapte↗

On Simulating the Impacts of Open Water Bodies on the SMAP Passive Soil Moisture Data Product

The Soil Moisture Active and Passive (SMAP) mission is a NASA earth science mission aiming at improving our understanding of the dynamics of the cycles of energy, water, and carbon at global scales. The mission features two complementary sensors on the same low-Earth orbiting platform: an L-band synthetic aperture radar (SAR) operating at 1.26 GHz and an L-band radiometer operating at 1.41 GHz. Together these instruments will provide global mapping of soil moisture and freeze/thaw states in 2-3 days, with a tentative launch date in 2014. The work reported in this study focuses primarily on the development of the SMAP radiometer-only soil moisture data product. For passive soil moisture retrieval at satellite footprint scales, one way to improve retrieval accuracy is to correct for the microwave emission from open water bodies prior to retrieval. The accuracy of this correction will depend on not only the locations of these water bodies, but also the geolocation accuracy of the instrument. As perfect knowledge is never attainable in practice, it is important to assess the impacts of these uncertainties on the SMAP radiometer observations and hence the passive soil moisture retrieval accuracy. In this presentation, we present the results of our preliminary assessment on the impacts of these uncertainties. Our study consists of two parts: (1) a sensitivity analysis on the SMAP radiometer observations due to uncertainties in water-body classification, and (2) realistic global simulations that take into account of additional uncertainties (e.g., geolocation and ancillary data) and SMAP-specific instrument characteristics (e.g., orbit sampling and antenna pattern). The results will provide valuable prelaunch guidance to the SMAP team in identifying different error sources and their relative impacts on the passive soil moisture data product.

Chan, Steven↗

A comparison of synthetic and measured solar continuum intensities and limb darkening coefficients

The paper compares recent center-limb studies of optical and IR continuum intensities (over the wavelength range 0.3-2.4 microns, with the data tabulated in the form of second- and fifth-order polynomial fits to empirical limb darkening curves) by Pierce and Slaughter (1977) and by Pierce et al. (1977) with predictions based on several current photospheric thermal structure models, using calibrated specific intensity measurements by Labs and Neckel (1968, 1970) to set an absolute temperature scale. The thermal structure in best quantitative agreement with the measurements of Pierce and Slaughter and Pierce et al. is the semiempirical model of Vernazza, Avrett and Loeser (1976). The temperatures in this model must be scaled upward, however, by a factor of 1.015 plus or minus 0.0005 to be consistent with the Labs and Neckel absolute calibration of continuum high points in the 0.40-0.65 micron region.

Ayres, T. R.↗

Synthetic Aperture Radar Technology Conference, New Mexico State University, Las Cruces, N. Mex., March 8-10, 1978, Proceedings

The following aspects of SAR development are discussed: calibration techniques, image simulation and interpretability, antennas, data processing, and system design. Papers are presented on such topics as a postlaunch calibration experiment for the Seasat-A SAR, computer simulation of an orbital SAR system, definition study of the Shuttle Imaging Radar, custom LSI circuits for spaceborne SAR processors, and random sampling adaptively focusing SAR.

Source record↗

VLSI system for synthetic aperture radar /SAR/ processing

The paper discusses an SAR problem based on actual requirements set forth by NASA for a spaceborne application. The requirements for high resolution and high quality necessitate a data sampling rate of 7.5 MHz. For each data value 1,025 4-bit complex multiply + add operations are needed, which is equivalent to 7.7 GHz complex multiply + add operation rate. Since this rate is much too high for general purpose systems, a special-purpose device was sought. This paper discusses two architectures based on parallel operation of 1,025 identical cells, each of which is capable of performing arithmetic, storage, and several control operations. The operation rate in each device is only 7.5 MHz, which is quite manageable, especially with the help of a substantial degree of pipelining. A computational-mathematical analysis is used as a primary tool for evaluating the design and some of its tradeoffs. Two different approaches are discussed and compared; both are based on having 1,025 identical cells working in parallel, but differ in their dual approaches to the flow of data.

Cohen, D.↗

Strength of anisotropic wood and synthetic materials

The possibility of using general formulas for determining the strength of different anisotropic materials is considered, and theoretical formulas are applied and confirmed by results of tests on various nonmetallic materials. Data are cited on the strength of wood, plywood, laminated wood plastics, fiber glass-reinforced plastics and directed polymer films.

Ashkenazi, Y. K.↗

Onboard Data Processor for Change-Detection Radar Imaging

A computer system denoted a change-detection onboard processor (CDOP) is being developed as a means of processing the digitized output of a synthetic-aperture radar (SAR) apparatus aboard an aircraft or spacecraft to generate images showing changes that have occurred in the terrain below between repeat passes of the aircraft or spacecraft over the terrain. When fully developed, the CDOP is intended to be capable of generating SAR images and/or SAR differential interferograms in nearly real time. The CDOP is expected to be especially useful for understanding some large-scale natural phenomena and/or mitigating natural hazards: For example, it could be used for near-real-time observation of surface changes caused by floods, landslides, forest fires, volcanic eruptions, earthquakes, glaciers, and sea ice movements. It could also be used to observe such longer-term surface changes as those associated with growth of vegetation (relevant to estimation of wildfire fuel loads). The CDOP is, essentially, an interferometric SAR processor designed to operate aboard a radar platform.

Lou, Yunling↗

Gravity waves in Titan's atmosphere

Scintillations (high frequency variations) observed in the radio signal during the occultation of Voyager 1 by Titan (Hinson and Tyler, 1983) provide information concerning neutral atmospheric density fluctuations on scales on hundreds of meters to a few kilometers. Those seen at altitudes higher than 25 km above the surface were interpreted by Hinson and Tyler as being caused by linear, freely propagating (energy-conserving) gravity waves, but this interpretation was found to be inconsistent with the scintillation data below the 25-km altitude level. Here an attempt is made to interpret the entire scintillation profile between the surface and the 90-km altitude level in terms of gravity waves generated at the surface. Numerical calculations of the density fluctuations caused by two-dimensional, nonhydrostatic, finite-amplitude gravity waves propagating vertically through Titan's atmosphere are performed to produce synthetic scintillation profiles for comparison with the observations. The numerical model accurately treats the effects of wave transience, nonlinearity, and breakdown due to convective instability in the overturned part of the wave. The high-altitude scintillation data were accurately recovered with a freely propagating wave solution, confirming the analytic model of Hinson and Tyler. It is found that the low-altitude scintillation data can be fit by a model where a component of the gravity waves becomes convectively unstable and breaks near the 15 km level. The large-scale structure of the observed scintillation profile in the entire altitude range between 5 and 85 km can be simulated by a model where the freely propagating and breaking waves are forced at the surface simultaneously. Further analysis of the Voyager 1 Titan low-altitude scintillation data, using inversion theory appropriate for strong scattering, could potentially remove some of the ambiguities remaining in this analysis and allow a better determination of the strength and source of the waves.

Friedson, A. James↗

Urban land use classification using synthetic aperture radar

Several approaches to the use of radar imagery for land use classification of urban and near-urban areas are presented. The use of L band, horizontal transmit, and horizontal receive data is emphasized because of their general availability. For urban area studies using imaging radar, the effects of processing in an off-zero Doppler or squint mode, of the presence of large diffuse scatters, and of the possibility of height measurements are discussed. Data from the Los Angeles area are illustratively used.

Bryan, M. L.↗

Doppler centroid estimation ambiguity for synthetic aperture radars

A technique for estimation of the Doppler centroid of an SAR in the presence of large uncertainty in antenna boresight pointing is described. Also investigated is the image degradation resulting from data processing that uses an ambiguous centroid. Two approaches for resolving ambiguities in Doppler centroid estimation (DCE) are presented: the range cross-correlation technique and the multiple-PRF (pulse repetition frequency) technique. Because other design factors control the PRF selection for SAR, a generalized algorithm is derived for PRFs not containing a common divisor. An example using the SIR-C parameters illustrates that this algorithm is capable of resolving the C-band DCE ambiguities for antenna pointing uncertainties of about 2-3 deg.

Chang, C. Y.↗

Elastohydrodynamic film thickness formula based on X-ray measurements with a synthetic paraffinic oil

An empirical elastohydrodynamic film thickness formula for heavily loaded contacts based upon X-ray film thickness measurements made with a synthetic paraffinic oil is presented. The deduced relation was found to adequately reflect the high load dependence exhibited by the measured minimum film thickness data at high Hertizian contact stresses, that is, above 1.04 x 10 to the ninth N/sq m (150,000 psi). Comparisons were made with the numerical results from a theoretical isothermal film thickness formula. The effects of changes in contact geometry, material, and lubricant properties on the form of the empirical model are also discussed.

Loewenthal, S. H.↗

Radar imaging from a spinning spacecraft

The feasibility of imaging the surface of Venus using a synthetic aperture radar in a spin stabilized Pioneer class spacecraft operating in an eccentric orbit is studied. Imaging radar fundamentals, constraints, power requirements and data processing considerations are reviewed. Additional effects due to operation from an elliptical orbit with a spinning spacecraft are covered. Recommended spin parameters are determined by simulations. Extensions to include noncoherent integration and stereo coverage are briefly reviewed. The results indicate that resolution on the order of 100 meters can be obtained from a 0.2 eccentricity orbit using a 2 meter antenna and reasonable transmitter power levels.

Bayma, R. W.↗

Information extraction and transmission techniques for spaceborne synthetic aperture radar images

Information extraction and transmission techniques for synthetic aperture radar (SAR) imagery were investigated. Four interrelated problems were addressed. An optimal tonal SAR image classification algorithm was developed and evaluated. A data compression technique was developed for SAR imagery which is simple and provides a 5:1 compression with acceptable image quality. An optimal textural edge detector was developed. Several SAR image enhancement algorithms have been proposed. The effectiveness of each algorithm was compared quantitatively.

Frost, V. S.↗

Remote sensing of tropical forests - An overview of research and applications using non-photographic sensors

A comprehensive overview is presented on how remote sensing technology has been applied to tropical forest monitoring over the past 20 years. Research needs for monitoring the condition and extent of tropical forests are suggested. The discussion focuses on nonphotographic sensors, especially those on orbiting satellites. Several remote sensing approaches to tropical forest monitoring are outlined, including NOAA AVHRR, Landsat MSS, the Landsat Thematic Mapper, SPOT-1, and Synthetic Aperture Radar. Suggested research needs are addressed, along with discussions on the use of Geographic Information Systems, and multistage and multisensor approaches in data analysis and acquisition. It is concluded that additional research and technique development is urgently needed to advance the utility of remotely sensed data for tropical forest monitoring. However, there is sufficient information available now to prototype a global tropical forest monitoring system that would utilize current satellite sensors complemented with airborne sensors for detailed measurements on sample locations.

Sader, Steven A.↗

Magellan project

The Magellan spacecraft was placed into orbit around Venus on 10 Aug. 1990 and started radar data acquisition on 15 Sep. 1990. Since then, Magellan has completed mapping over 2.75 rotations of the planet (as of mid-July 1992). Synthetic aperture radar (SAR), altimetry, and radiometry observations have covered 84 percent of the surface during the first mission cycle from mid-Sep. 1990 through mid-May 1991. Operations in the second mission cycle from mid-May 1991 through mid-Jan. 1992 emphasized filling the larger gaps (the south polar region and a superior conjunction) from that first cycle. Planned observations in the fourth mission cycle from mid-Sep. 1992 through mid-May 1993 will emphasize high-resolution gravity observations of the equatorial regions of Venus.

Scott, J. F.↗

Optimal Estimation Of Range And Doppler Centroid In ScanSAR

Algorithm processes synthetic-aperture-radar (SAR) returns into optimal estimates of ranges, pointing angles, and Doppler centroids. Derives radar-beam-pointing angles partly from SAR data. Exploits information available in overlapping regions between adjacent image bursts or, equivalently, between adjacent radar beams corresponding to adjacent image bursts. Range or Doppler centroid obtained from estimate of range or Doppler value at which intensity in one image burst equals that in adjacent burst.

Jin, Michael Y.↗

Spectroscopy Made Easy: A New Tool for Fitting Observations with Synthetic Spectra

We describe a new software package that may be used to determine stellar and atomic parameters by matching observed spectra with synthetic spectra generated from parameterized atmospheres. A nonlinear least squares algorithm is used to solve for any subset of allowed parameters, which include atomic data (log gf and van der Waals damping constants), model atmosphere specifications (T(sub eff, log g), elemental abundances, and radial, turbulent, and rotational velocities. LTE synthesis software handles discontiguous spectral intervals and complex atomic blends. As a demonstration, we fit 26 Fe I lines in the NSO Solar Atlas (Kurucz et al.), determining various solar and atomic parameters.

Valenti, J. A.↗