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At least 1,207 records · Page 67
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

X-ray chemical imaging for assessing redox microsites within soils and sediments

Redox reactions underlie several biogeochemical processes and are typically spatiotemporally heterogeneous in soils and sediments. However, redox heterogeneity has yet to be incorporated into mainstream conceptualizations and modeling of soil biogeochemistry. Anoxic microsites, a defining feature of soil redox heterogeneity, are non-majority oxygen depleted zones in otherwise oxic environments. Neglecting to account for anoxic microsites can generate major uncertainties in quantitative assessments of greenhouse gas emissions, C sequestration, as well as nutrient and contaminant cycling at the ecosystem to global scales. However, only a few studies have observed/characterized anoxic microsites in undisturbed soils, primarily, because soil is opaque and microsites require µm-cm scale resolution over cm-m scales. Consequently, our current understanding of microsite characteristics does not support model parameterization. To resolve this knowledge gap, we demonstrate through this proof-of-concept study that X-ray fluorescence (XRF) 2D mapping can reliably detect, quantify, and provide basic redox characterization of anoxic microsites using solid phase “forensic” evidence. First, we tested and developed a systematic data processing approach to eliminate false positive redox microsites, i.e., artefacts, detected from synchrotron-based multiple-energy XRF 2D mapping of Fe (as a proxy of redox-sensitive elements) in Fe-“rich” sediment cores with artificially injected microsites. Then, spatial distribution of Fe II and Fe III species from full, natural soil core slices (over cm-m lengths/widths) were mapped at 1–100 µm resolution. These investigations revealed direct evidence of anoxic microsites in predominantly oxic soils such as from an oak savanna and toeslope soil of a mountainous watershed, where anaerobicity would typically not be expected. We also revealed preferential spatial distribution of redox microsites inside aggregates from oak savanna soils. We anticipate that this approach will advance our understanding of soil biogeochemistry and help resolve “anomalous” occurrences of reduced products in nominally oxic soils.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2020

Measurement of flow properties coupled to experimental and numerical analyses of dense, granular flows for solar thermal energy storage

Granular flows of sintered bauxite proppants were researched along an inclined plane for solar thermal energy storage applications. Granular flow properties needed to drive numeric granular models were measured for improved numerical model predictions for Carbobead CP 50/140, 40/100, and 30/60 particles. Particle shape and size distributions were determined by coupling optical microscopy to an in-house image processing algorithm. The impulse excitation technique was used to measure elastic and shear moduli, and compute Poisson’s ratio. The coefficient of static sliding friction was measured using the slip-stick method, and the static rolling friction was determined from measured shear on particles positioned between two hot-pressed plates. The coefficient of restitution was measured by dropping particles on a surface and determining the kinetic energy before and after impact with the surface using high resolution particle tracking velocimetry. Particle size did not significantly impact the coefficients of restitution and static rolling friction, however, particle shape distribution resulted in a large variation in measurements. An inclined flow experiment was performed to characterize granular flows of Carbobead CP 30/60 particles using particle image velocimetry. Numerical models of the experiment using discrete element method were generated with the measured mechanical properties as inputs for comparison with experimental results. A constant directional torque rolling friction model best predicted bulk granular flow behavior. Furthermore, good agreement between the model and experiment was achieved at ambient, steady state conditions, with average velocity differences <10%.

14 SOLAR ENERGY↗
DOE OSTI2020

Sarecycline interferes with tRNA accommodation and tethers mRNA to the 70S ribosome

Sarecycline is a new narrow-spectrum tetracycline-class antibiotic approved for the treatment of acne vulgaris. Tetracyclines share a common four-ring naphthacene core and inhibit protein synthesis by interacting with the 70S bacterial ribosome. Sarecycline is distinguished chemically from other tetracyclines because it has a 7-[[methoxy(methyl)amino]methyl] group attached at the C7 position of ring D. To investigate the functional role of this C7 moiety, we determined the X-ray crystal structure of sarecycline bound to the Thermus thermophilus 70S ribosome. Our 2.8-Å resolution structure revealed that sarecycline binds at the canonical tetracycline binding site located in the decoding center of the small ribosomal subunit. Importantly, unlike other tetracyclines, the unique C7 extension of sarecycline extends into the messenger RNA (mRNA) channel to form a direct interaction with the A-site codon to possibly interfere with mRNA movement through the channel and/or disrupt A-site codon–anticodon interaction. Based on our biochemical studies, sarecycline appears to be a more potent initiation inhibitor compared to other tetracyclines, possibly due to drug interactions with the mRNA, thereby blocking accommodation of the first aminoacyl transfer RNA (tRNA) into the A site. Overall, our structural and biochemical findings rationalize the role of the unique C7 moiety of sarecycline in antibiotic action.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

SCExAO/CHARIS Near-infrared Integral Field Spectroscopy of the HD 15115 Debris Disk

We present new, near-infrared (1.1–2.4 μm) high-contrast imaging of the debris disk around HD 15115 with the Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) system coupled with the Coronagraphic High Angular Resolution Imaging Spectrograph (CHARIS). The SCExAO/CHARIS resolves the disk down to ρ ∼ 0.″2 (r {sub proj} ∼ 10 au), a factor of ∼3–5 smaller than previous recent studies. We derive a disk position angle of PA ∼ 279.°4–280.°5 and an inclination of i ∼ 85.°3–86.2.°. While recent SPHERE/IRDIS imagery of the system could suggest a significantly misaligned two-ring disk geometry, CHARIS imagery does not reveal conclusive evidence for this hypothesis. Moreover, optimizing models of both one- and two-ring geometries using differential evolution, we find that a single ring having a Hong-like scattering phase function matches the data equally well within the CHARIS field of view (ρ ≲ 1″). The disk’s asymmetry, well evidenced at larger separations, is also recovered; the west side of the disk appears, on average, around 0.4 mag brighter across the CHARIS bandpass between 0.″25 and 1″. Comparing Space Telescope Imaging Spectrograph (STIS) 50CCD optical photometry (2000–10500 Å) with CHARIS near-infrared photometry, we find a red (STIS/50CCD−CHARIS broadband) color for both sides of the disk throughout the 0.″4–1″ region of overlap, in contrast to the blue color reported at similar wavelengths for regions exterior to ∼2″. Further, this color may suggest a smaller minimum grain size than previously estimated at larger separations. Finally, we provide constraints on planetary companions and discuss possible mechanisms for the observed inner disk flux asymmetry and color.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

Super-localisation of a point-like emitter in a resonant environment: Correction of the mirage effect

In this paper, we show that it is possible to overcome one of the fundamental limitations of super-resolution microscopy: the necessity to be in an optically homogeneous environment. Using recent modal approximation results from [ 10 , 7 ], we show, as a proof of concept, that it is possible to recover the position of a single point-like emitter in a known resonant environment from far-field measurements, with a precision two orders of magnitude below the classical Rayleigh limit. The procedure does not involve solving any partial differential equation, is computationally light (optimisation in \begin{document}$ \mathbb{R}^d $\end{document} with \begin{document}$ d $\end{document} of the order of \begin{document}$ 10 $\end{document} ) and is therefore suited for the recovery of a very large number of single emitters.

Mathematics↗
NASA NTRS1974

The nucleus of M31

The nucleus of M31 was photographed at a resolution of 0.2 sec with the 91-cm balloon-borne Stratoscope II telescope. At the half-intensity level, the nucleus was observed to be elliptical with its major axis lying in position angle of 63 (plus or minus 5) deg. The peak surface brightness was 12.7 plus or minus 0.3 V mag per square second of arc assuming B - V = 1.0. The nucleus appears to be a separate feature from the bulge with a scale height of approximately 0.5 pc. The mass of the nucleus is of the order of 100 million solar masses, and the apparent visual mass-to-light ratio is of the order of 20.

Light, E. S.↗
NASA NTRS1979

The coronal atmosphere above solar active regions - Comparison of high spatial resolution soft X-ray and centimetric observations

High spatial resolution observations of solar active regions in soft X-rays and centimetric wavelengths are compared using X-ray and radio data obtained during the 1973 Skylab mission. An overall correspondence in position and size between regions of enhanced X-ray emission and regions of enhanced microwave emission was noticed. However, a closer analysis of the findings suggested that substantial differences exist between the emission properties of the atmosphere over sunspots and that over plages, with the difference probably related to the average intensity of the magnetic field, which was found to be higher over sunspot umbrae than over plage areas.

Pallavicini, R.↗
NASA NTRS1980

Photoelectric scanning /6.65-m/ spectrometer for VUV cross-section measurements

A 6.65-m normal incidence vacuum spectrograph has been provided with a photoelectric scanner that is easily interchangeable with the original plate holder. The scanner consists essentially of a photoelectric detector and an exit slit that scans mechanically along the focal surface while the grating and entrance slit remain stationary. The design and construction of the 6.65-m scanning spectrometer, which operates in the 120-300-nm wavelength region, are discussed. The wavelength resolution of 0.001 nm obtained photoelectrically is better than that of any existing vacuum monochromator. The performance of the instrument is illustrated with vacuum ultraviolet scans of the (1, 5) band of fourth positive system of CO in emission, and the (14, 0) band of the Schumann-Runge system of O2 in absorption.

Yoshino, K.↗
NASA NTRS1980

Observations of chromospheric lines from OSO-8

Results of OSO-8 measurements of the line profiles of the solar Lyman alpha, Ca II K and Mg II k lines are presented. Observations were obtained with the Laboratoire de Physique Stellaire et Planetaire spectrometer at spectral resolutions of 0.25 and 0.060 A for Lyman alpha and 0.025 A for the Mg II and Ca II lines. The Lyman alpha profiles are found to be highly variable according to spatial position with the intensities of the three lines well correlated, and confirm previous observations of the quiet solar chromosphere. Data suggest that the quiet chromosphere is a dynamical phenomenon whose description in terms of a static model atmosphere is only qualitatively valid at best.

Grossmann-Doerth, U.↗
NASA NTRS1980

A proposed method for wind velocity measurement from space

An investigation was made of the feasibility of making wind velocity measurements from space by monitoring the apparent change in the refractive index of the atmosphere induced by motion of the air. The physical principle is the same as that resulting in the phase changes measured in the Fizeau experiment. It is proposed that this phase change could be measured using a three cornered arrangement of satellite borne source and reflectors, around which two laser beams propagate in opposite directions. It is shown that even though the velocity of the satellites is much larger than the wind velocity, factors such as change in satellite position and Doppler shifts can be taken into account in a reasonable manner and the Fizeau phase measured. This phase measurement yields an average wind velocity along the ray path through the atmosphere. The method requires neither high accuracy for satellite position or velocity, nor precise knowledge of the refractive index or its gradient in the atmosphere. However, the method intrinsically yields wind velocity integrated along the ray path; hence to obtain higher spatial resolution, inversion techniques are required.

Censor, D.↗
NASA NTRS1986

A comparison of high-resolution radio and optical data for the northeast region of the Cygnus Loop

Radio observations of the northeastern part of the Cygnus Loop obtained with the VLA at 18 cm with a resolution of 4.7 arcsec are presented and discussed. In general, the brightest radio filaments match the brightest optical filaments very well, particularly those which are bright in H-alpha. The agreement in position, morphology, and relative brightness is particularly good where all four optical species are bright. However, a few bright radio features have no bright optical counterparts. The general radio and optical structure of the region can be explained by a model in which shocked interstellar clouds form filaments due to unstable cooling and compression. The features appear to be ropelike, although it is not possible to rule out that they are irregular sheets seen edge-on. A large gradation in cooling on a scale of parsecs is evidenced by the sequence of the optical species in emission, with a corresponding increase in density generally evidenced by the continuum radio emission.

Straka, W. C.↗
NASA NTRS1986

Intelligent information extraction from reflectance spectra Absorption band positions

A multiple high-order derivative analysis algorithm has been developed which can automatically extract absorption band positions from low-quality reflectance spectra with little degredation of accuracy. Overlapping bands with comparable widths and intensities can be resolved whose centers are as close as 0.3-0.5 W, with safer resolution limits of 0.6-1.0 W band center separations suggested for overlapping bands that are dissimilar. The segment length for smoothing is continually adjusted to about 0.5 W to minimize signal distortion, and a spectral pattern recognition algorithm predicts the signal spectrum and calculates approximate W across the spectrum using its second derivative. A single-pass cubic spline is applied to the smoothed data, and a sliding segment sixth-order polynomial is fit to the spectrum, with the length of the segment being continuously locally adjusted to 1.0 W across the spectrum. Good reliability and consistency of the algorithm is demonstrated with application to laboratory and earth-based telescope spectra.

Huguenin, R. L.↗
NASA NTRS1988

High-resolution forbidden Ne II observations of the ionized filaments in the Galactic center

The forbidden Ne II 12.8 micron emission from the central 10 arcsec of the Galaxy has been observed with 1.5 arcsec resolution. The broad lines previously observed in this region are resolved into separate features associated with the filaments and knots of radio continuum emission present. The radial velocity varies rapidly and continuously with position along the filaments, with gradients of up to 30 km/s. The emission along the 'eastern arm' is mapped. The velocities are consistent with streams of gas orbiting about the center. A simple model of gravitationally dominated orbits fits the data; the masses estimated to lie within the orbits suggest the presence of a central mass of about 2.5 million solar. The implications of this model and possible objections to it are considered.

Serabyn, E.↗
NASA NTRS1989

The Einstein X-ray Observatory Catalog of O-type stars

A catalog of X-ray count rates for all O-type stars surveyed by the Einstein Observatory is presented. The procedures by which the IPC and High Resolution Imager rates are converted to emitted X-ray luminosities are discussed in detail. The catalog contains 289 stars with 89 detections and 176 upper bounds for the observed count rates. Precise positions for 43 O-type stars are given whose previously known locations were unknown or erroneous. It is argued that most O-type stars are X-ray point sources, but some are possibly embedded in extended nebulae and/or bubbles. The X-ray spectra of O-type stars are discussed briefly, and it is argued that their mean spectrum can reasonably be taken as an 0.5 keV thermal bremsstrahlung model. It is argued that at least 30 percent of all O-type stars are variable in X-rays.

Chlebowski, T.↗
NASA NTRS1989

Non-LTE analysis of extremely helium-rich stars. The hot sdO stars LSE 153, 259 and 263

Results of a non-LTE fine analysis based mainly on high-resolution CASPEC spectra for three extremely helium-rich sdO stars are discussed in order to explain hydrogen deficiency in single stars. High temperature (Teff = 70,000 to 75,000 K) and a position in the log Teff - log g diagram were found close to the Eddington limit. Various abundance estimates are derived for hydrogen (upper limits only), carbon, nitrogen, and magnesium. Hydrogen is reduced to less than 10 percent by number in LSE 153 and LSE 263, and to less than 5 percent in LSE 259. The hydrogen deficiency is accompanied by nitrogen- and carbon-enrichment in LSE 153 and LSE 259 only. In LSE 263, carbon is depleted by about 1 dex. Stellar masses obtained by assuming that a core mass-luminosity relation holds for these stars, were found to be in the range 0.6-0.9 solar mass, yielding luminosities log L/L:solar = 3.7-4.5. Two of the program stars (LSE 153 and 259) appear to be possible successors of the R CrB and helium B stars, whereas the third star (LSE 263) displays a much lower carbon content in its photosphere making it an exceptional case among the known hydrogen deficient stars.

Husfeld, D.↗
NASA NTRS1990

Line parameters for ozone hot bands in the 4.8-micron spectral region

Line positions, intensities, and lower-state energies have been calculated for nine hot bands of (O-16)3 in the 4.8-micron spectral region using improved spectroscopic parameters deduced in recent high-resolution laboratory studies. The good quality of the hot-band parameters has been verified through comparisons of line-by-line simulations with 0.005/cm-resolution laboratory spectra of ozone. The present work and the line parameters calculated for the main bands by Pickett et al. (1988) provide a complete update of ozone spectroscopic parameters in the 4.8 micron region.

Camy-Peyret, Claude↗
NASA NTRS1990

Variable magnification glancing incidence x ray telescope

A multispectral glancing incidence x ray telescope is disclosed, which capable of broadband, high resolution imaging of solar and stellar x ray and extreme ultraviolet radiation sources includes a primary optical system which focuses the incoming radiation to a primary focus. Two or more ellipsoidal mirrors are positioned behind the primary focus at an inclination to the optical axis, each mirror having a concave surface coated with a multilayer synthetic microstructure coating to reflect a desired wavelength. The ellipsoidal mirrors are segments of respective ellipsoids having a common first focus coincident with the primary focus. A detector such as an x ray sensitive photographic film is positioned at the second focus of each of the ellipsoids so that each of the ellipsoidal mirrors may reflect the image at the first focus to the detector. In one embodiment the mirrors are inclined at different angles and has its respective second focus at a different location, separate detectors being located at the respective second focus. The mirrors are arranged so that the magnification and field of view differ, and a solenoid activated arm may withdraw at least one mirror from the beam to select the mirror upon which the beam is to impinge so that selected magnifications and fields of view may be detected.

Hoover, Richard↗