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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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An RNA-Seq analysis of Francisella tularensis LVS transcriptional shifts at early time points during in vitro infections.
Abstract not provided.
A World-to-Digital Microfluidic Interface for Whole Blood RNA Preparation.
Abstract not provided.
A capture-based technique for enhancing RNA-Seq analysis of bacterial transcriptomes during in vitro and in vivo infections.
Abstract not provided.
Peregrine: A rapid and unbiased method to produce strand-specific RNA-seq libraries from small quantities of starting material.
Abstract not provided.
RNA-guided transcriptional regulation
Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.
A Capture-Based Technique for Enhancing RNA-Seq Analysis of Bacterial Transcriptomes During in vitro and in vivo Infections.
Abstract not provided.
A Capture-Based Technique for Enhancing RNA-Seq Analyses of Bacterial Transcriptomes During In Vitro and In Vivo Infections.
Abstract not provided.
MICROFLUIDIC DEVICE FOR RAPID SIMULTANEOUS DETECTION OF DNA RNA AND PROTEIN BIOMARKERS IN SINGLE CELLS.
Abstract not provided.
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
A Capture-Based Technique for Enhancing RNA-Seq Analyses of Bacterial Transcriptomes During In Vitro and In Vivo Infections.
Abstract not provided.
A capture-based technique for enhancing RNA-seq analysis of bacterial transcriptomes during in vitro and in vivo infections.
Abstract not provided.
Microfluidic Device for Rapid Simultaneous Detection of DNA RNA and Protein Biomarkers in Single Cells.
Abstract not provided.