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NASA NTRS2004

Modeling and Design Analysis Methodology for Tailoring of Aircraft Structures with Composites

Composite materials provide design flexibility in that fiber placement and orientation can be specified and a variety of material forms and manufacturing processes are available. It is possible, therefore, to 'tailor' the structure to a high degree in order to meet specific design requirements in an optimum manner. Common industrial practices, however, have limited the choices designers make. One of the reasons for this is that there is a dearth of conceptual/preliminary design analysis tools specifically devoted to identifying structural concepts for composite airframe structures. Large scale finite element simulations are not suitable for such purposes. The present project has been devoted to creating modeling and design analysis methodology for use in the tailoring process of aircraft structures. Emphasis has been given to creating bend-twist elastic coupling in high aspect ratio wings or other lifting surfaces. The direction of our work was in concert with the overall NASA effort Twenty- First Century Aircraft Technology (TCAT). A multi-disciplinary team was assembled by Dr. Damodar Ambur to work on wing technology, which included our project.

Rehfield, Lawrence W.↗
NASA NTRS2003

Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs

The General Fluorescence Plant Meter (GFP-Meter) is a portable spectrofluorometer that utilizes a fiber-optic cable and a leaf clip to gather spectrofluorescence data. In contrast to traditional analytical systems, this instrument allows for the rapid detection and fluorescence measurement of proteins under field conditions with no damage to plant tissue. Here we discuss the methodology of gathering and standardizing spectrofluorescence data from tobacco and canola plants expressing GFP. Furthermore, we demonstrate the accuracy and effectiveness of the GFP-Meter. We first compared GFP fluorescence measurements taken by the GFP-Meter to those taken by a standard laboratory-based spectrofluorometer, the FluoroMax-2. Spectrofluorescence measurements were taken from the same location on intact leaves. When these measurements were tested by simple linear regression analysis, we found that there was a positive functional relationship between instruments. Finally, to exhibit that the GFP-Meter recorded accurate measurements over a span of time, we completed a time-course analysis of GFP fluorescence measurements. We found that only initial measurements were accurate; however, subsequent measurements could be used for qualitative purposes.

Evaluation Studies↗
NASA NTRS1999

Novel optical methodologies in studying mechanical signal transduction in mammalian cells

For the last 3 decades evidence has been accumulating that some types of mammalian cells respond to their mechanically active environment by altering their morphology, growth rate, and metabolism. The study of such responses is very important in understanding, physiological and pathological conditions ranging from bone formation to atherosclerosis. Obtaining this knowledge has been the goal for an active research area in bioengineering termed cell mechanotransduction. The advancement of optical methodologies used in cell biology research has given the tools to elucidate cellular mechanisms that would otherwise be impossible to visualize. Combined with molecular biology techniques, they give engineers invaluable tools in understanding the chemical pathways involved in mechanotransduction. Herein we briefly review the current knowledge on mechanical signal transduction in mammalian cells, focusing on the application of novel optical techniques in the ongoing research.

NASA Discipline Cell Biology↗
NASA NTRS1996

A system and methodology for measuring volatile organic compounds produced by hydroponic lettuce in a controlled environment

A system and methodology were developed for the nondestructive qualitative and quantitative analysis of volatile emissions from hydroponically grown 'Waldmann's Green' leaf lettuce (Lactuca sativa L.). Photosynthetic photon flux (PPF), photoperiod, and temperature were automatically controlled and monitored in a growth chamber modified for the collection of plant volatiles. The lipoxygenase pathway products (Z)-3-hexenal, (Z)-3-hexenol, and (Z)-3-hexenyl acetate were emitted by lettuce plants after the transition from the light period to the dark period. The volatile collection system developed in this study enabled measurements of volatiles emitted by intact plants, from planting to harvest, under controlled environmental conditions.

NASA Discipline Life Support Systems↗
NASA NTRS1986

Review of methodology and technology available for the detection of extrasolar planetary systems

Anyone undertaking an interstellar voyage might wish to be assured of the existence of a safe planetary harbor at the other end! Aside from the obvious interest of the participants in this Symposium, astronomers and astrophysicists are also eager to detect and study other planetary systems in order to better understand the formation of our own Solar System. Scientists involved in the search for extraterrestrial intelligence argue that planets suitable for the evolution of life may abound elsewhere within our own Milky Way Galaxy. On theoretical grounds, they are probably correct, but they lack any observational support. For in spite of decades of claimed astrometric detections of planetary companions and the recent exciting and tantalizing observations from the IRAS satellite and the IR speckle observations of Van Biesbroeck 8 and other cool stars, there is no unambiguous proof for the existence of another planetary system beyond our own. In this paper we review the various methods for detecting extrasolar planets and briefly describe the Earth and space based technology currently available and discuss the near-term plans to implement these different search techniques. In each case an attempt is made to identify the limiting source of systematic error inherent to the methodology and to assess the potential for technological improvements.

Non-NASA Center↗
NASA NTRS1997

Evaluation of a Progressive Failure Analysis Methodology for Laminated Composite Structures

A progressive failure analysis methodology has been developed for predicting the nonlinear response and failure of laminated composite structures. The progressive failure analysis uses C plate and shell elements based on classical lamination theory to calculate the in-plane stresses. Several failure criteria, including the maximum strain criterion, Hashin's criterion, and Christensen's criterion, are used to predict the failure mechanisms. The progressive failure analysis model is implemented into a general purpose finite element code and can predict the damage and response of laminated composite structures from initial loading to final failure.

Sleight, David W.↗
NASA NTRS1996

DSMC Grid Methodologies for Computing Low-Density, Hypersonic Flows About Reusable Launch Vehicles

Two different grid methodologies are studied for application to DSMC simulations about reusable launch vehicles. One method uses an unstructured, tetrahedral grid while the other uses a structured, variable-resolution Cartesian grid. The relative merits of each method are discussed in terms of accuracy, computational efficiency, and overall ease of use. Both methods are applied to the computation of a low-density, hypersonic flow about a winged single-stage-to-orbit reusable launch vehicle concept at conditions corresponding to an altitude of 120 km. Both methods are shown to give comparable results for both surface and flowfield quantities as well as for the overall aerodynamic behavior. For the conditions simulated, the flowfield about the vehicle is very rarefied but the DSMC simulations show significant departure from free-molecular predictions for the surface friction and heat transfer as well as certain aerodynamic quantities.

Wilmoth, Richard G.↗
NASA NTRS2004

An Evaluation Methodology for Traffic Awareness Displays

An evaluation methodology for traffic awareness displays for helicopters and other vertical/short takeoff aircraft was developed. Pilots of vertical/short takeoff aircraft wil1 require more traffic information than would pilots of conventional aircraft to avoid both other vertical/short takeoff traffic and conventional traffic. The BF Goodrich Skywatch traffic advisory display was used as a candidate display to develop a procedure for evaluating the usefulness of such displays. Four high-time helicopter pilots participated in a 16-hour flight evaluation. They flew a closed circuit in the San Francisco Bay Area. In one-half of the flights the evaluation pilot had the traffic advisory display as an aid in detecting and locating traffic. In the other half of the flights the traffic advisory display was not available to the evaluation pilot. Data examined include measures of traffic advisory display performance and pilot performance in detecting traffic, as well as subjective workload and situation awareness data. The traffic advisory system did not help the pilots to detect more traffic. The importance of detection to traffic awareness is discussed.

DeMaio, Joe↗
NASA NTRS2004

MODIS In-flight Calibration Methodologies

MODIS is a key instrument for the NASA's Earth Observing System (EOS) currently operating on the Terra spacecraft launched in December 1999 and Aqua spacecraft launched in May 2002. It is a cross-track scanning radiometer, making measurements over a wide field of view in 36 spectral bands with wavelengths from 0.41 to 14.5 micrometers and providing calibrated data products for science and research communities in their studies of the Earth s system of land, oceans, and atmosphere. A complete suite of on-board calibrators (OBC) have been designed for the instruments in-flight calibration and characterization, including a solar diffuser (SD) and solar diffuser stability monitor (SDSM) system for the radiometric calibration of the 20 reflective solar bands (RSB), a blackbody (BB) for the radiometric calibration of the 16 thermal emissive bands (TEB), and a spectro-radiometric calibration assembly (SRCA) for the spatial (all bands) and spectral (RSB only) characterization. This paper discusses MODIS in-flight Cali bration methodologies of using its on-board calibrators. Challenging issues and examples of tracking and correcting instrument on-orbit response changes are presented, including SD degradation (20% at 412nm, 12% at 466nm, and 7% at 530nm over four and a half years) and response versus scan angle changes (10%, 4%, and 1% differences between beginning of the scan and end of the scan at 412nm, 466nm, and 530nm) in the VIS spectral region. Current instrument performance and lessons learned are also provided.

Xiong, X.↗
NASA NTRS1997

Laser-assisted patch clamping: a methodology

Laser microsurgery can be used to perform both cell biological manipulations, such as targeted cell ablation, and molecular genetic manipulations, such as genetic transformation and chromosome dissection. In this report, we describe a laser microsurgical method that can be used either to ablate single cells or to ablate a small area (1-3 microns diameter) of the extracellular matrix. In plants and microorganisms, the extracellular matrix consists of the cell wall. While conventional patch clamping of these cells, as well as of many animal cells, requires enzymatic digestion of the extracellular matrix, we illustrate that laser microsurgery of a portion of the wall enables patch clamp access to the plasma membrane of higher plant cells remaining situated in their tissue environment. What follows is a detailed description of the construction and use of an economical laser microsurgery system, including procedures for single cell and targeted cell wall ablation. This methodology will be of interest to scientists wishing to perform cellular or subcellular ablation with a high degree of accuracy, or wishing to study how the extracellular matrix affects ion channel function.

Non-NASA Center↗
NASA NTRS2004

Angular Distribution Models for Top-of-Atmosphere Radiative Flux Estimation from the Clouds and the Earth's Radiant Energy System Instrument on the Terra Satellite: Methodology - Part 1

The Clouds and Earth's Radiant Energy System (CERES) provides coincident global cloud and aerosol properties together with reflected solar, emitted terrestrial longwave and infrared window radiative fluxes. These data are needed to improve our understanding and modeling of the interaction between clouds, aerosols and radiation at the top of the atmosphere, surface, and within the atmosphere. This paper describes the approach used to estimate top-of-atmosphere (TOA) radiative fluxes from instantaneous CERES radiance measurements on the Terra satellite. A key component involves the development of empirical angular distribution models (ADMs) that account for the angular dependence of Earth's radiation field at the TOA. The CERES Terra ADMs are developed using 24 months of CERES radiances, coincident cloud and aerosol retrievals from the Moderate Resolution Imaging Spectroradiometer (MODIS), and meteorological parameters from the Global Modeling and Assimilation Office (GMA0) s Goddard Earth Observing System DAS (GEOS-DAS V4.0.3) product. Scene information for the ADMs is from MODIS retrievals and GEOS-DAS V4.0.3 properties over ocean, land, desert and snow, for both clear and cloudy conditions. Because the CERES Terra ADMs are global, and far more CERES data is available on Terra than was available from CERES on the Tropical Rainfall Measuring Mission (TRMM), the methodology used to define CERES Terra ADMs is different in many respects from that used to develop CERES TRMM ADMs, particularly over snow/sea-ice, under cloudy conditions, and for clear scenes over land and desert.

Loeb, N. G.↗
NASA NTRS2005

Numerical Prediction of Chevron Nozzle Noise Reduction using Wind-MGBK Methodology

Numerical predictions for single-stream chevron nozzle flow performance and farfield noise production are presented. Reynolds Averaged Navier Stokes (RANS) solutions, produced via the WIND flow solver, are provided as input to the MGBK code for prediction of farfield noise distributions. This methodology is applied to a set of sensitivity cases involving varying degrees of chevron inward bend angle relative to the core flow, for both cold and hot exhaust conditions. The sensitivity study results illustrate the effect of increased chevron bend angle and exhaust temperature on enhancement of fine-scale mixing, initiation of core breakdown, nozzle performance, and noise reduction. Direct comparisons with experimental data, including stagnation pressure and temperature rake data, PIV turbulent kinetic energy fields, and 90 degree observer farfield microphone data are provided. Although some deficiencies in the numerical predictions are evident, the correct farfield noise spectra trends are captured by the WIND-MGBK method, including the noise reduction benefit of chevrons. Implications of these results to future chevron design efforts are addressed.

Engblom, W.A.↗
NASA NTRS1996

Methodology Developed for Modeling the Fatigue Crack Growth Behavior of Single-Crystal, Nickel-Base Superalloys

Because of their superior high-temperature properties, gas generator turbine airfoils made of single-crystal, nickel-base superalloys are fast becoming the standard equipment on today's advanced, high-performance aerospace engines. The increased temperature capabilities of these airfoils has allowed for a significant increase in the operating temperatures in turbine sections, resulting in superior propulsion performance and greater efficiencies. However, the previously developed methodologies for life-prediction models are based on experience with polycrystalline alloys and may not be applicable to single-crystal alloys under certain operating conditions. One of the main areas where behavior differences between single-crystal and polycrystalline alloys are readily apparent is subcritical fatigue crack growth (FCG). The NASA Lewis Research Center's work in this area enables accurate prediction of the subcritical fatigue crack growth behavior in single-crystal, nickel-based superalloys at elevated temperatures.

Source record↗
NASA NTRS2005

Rocket Engine Nozzle Side Load Transient Analysis Methodology: A Practical Approach

During the development stage, in order to design/to size the rocket engine components and to reduce the risks, the local dynamic environments as well as dynamic interface loads must be defined. There are two kinds of dynamic environment, i.e. shock transients and steady-state random and sinusoidal vibration environments. Usually, the steady-state random and sinusoidal vibration environments are scalable, but the shock environments are not scalable. In other words, based on similarities only random vibration environments can be defined for a new engine. The methodology covered in this paper provides a way to predict the shock environments and the dynamic loads for new engine systems and new engine components in the early stage of new engine development or engine nozzle modifications.

Shi, John J.↗
NASA NTRS2005

Rocket Engine Nozzle Side Load Transient Analysis Methodology: A Practical Approach

At the sea level, a phenomenon common with all rocket engines, especially for a highly over-expanded nozzle, during ignition and shutdown is that of flow separation as the plume fills and empties the nozzle, Since the flow will be separated randomly. it will generate side loads, i.e. non-axial forces. Since rocket engines are designed to produce axial thrust to power the vehicles, it is not desirable to be excited by non-axial input forcing functions, In the past, several engine failures were attributed to side loads. During the development stage, in order to design/size the rocket engine components and to reduce the risks, the local dynamic environments as well as dynamic interface loads have to be defined. The methodology developed here is the way to determine the peak loads and shock environments for new engine components. In the past it is not feasible to predict the shock environments, e.g. shock response spectra, from one engine to the other, because it is not scaleable. Therefore, the problem has been resolved and the shock environments can be defined in the early stage of new engine development. Additional information is included in the original extended abstract.

Shi, John J.↗
NASA NTRS2005

Three-Dimensional Application of DAMAS Methodology for Aeroacoustic Noise Source Definition

At the 2004 AIAA/CEAS Aeroacoustic Conference, a breakthrough in acoustic microphone array technology was reported by the authors. A Deconvolution Approach for the Mapping of Acoustic Sources (DAMAS) was developed which decouples the array design and processing influence from the noise being measured, using a simple and robust algorithm. For several prior airframe noise studies, it was shown to permit an unambiguous and accurate determination of acoustic source position and strength. As a follow-on effort, this paper examines the technique for three-dimensional (3D) applications. First, the beamforming ability for arrays, of different size and design, to focus longitudinally and laterally is examined for a range of source positions and frequency. Advantage is found for larger array designs with higher density microphone distributions towards the center. After defining a 3D grid generalized with respect to the array s beamforming characteristics, DAMAS is employed in simulated and experimental noise test cases. It is found that spatial resolution is much less sharp in the longitudinal direction in front of the array compared to side-to-side lateral resolution. 3D DAMAS becomes useful for sufficiently large arrays at sufficiently high frequency. But, such can be a challenge to computational capabilities, with regard to the required expanse and number of grid points. Also, larger arrays can strain basic physical modeling assumptions that DAMAS and all traditional array methodologies use. An important experimental result is that turbulent shear layers can negatively impact attainable beamforming resolution. Still, the usefulness of 3D DAMAS is demonstrated by the measurement of landing gear noise source distributions in a difficult hard-wall wind tunnel environment.

Brooks, Thomas F.↗
NASA NTRS2005

Microbiological Methodology in Astrobiology

Searching for life in astromaterials to be delivered from the future missions to extraterrestrial bodies is undoubtedly related to studies of the properties and signatures of living microbial cells and microfossils on Earth. As model terrestrial analogs of Martian polar subsurface layers are often regarded the Antarctic glacier and Earth permafrost habitats where alive microbial cells preserved viability for millennia years due to entering the anabiotic state. For the future findings of viable microorganisms in samples from extraterrestrial objects, it is important to use a combined methodology that includes classical microbiological methods, plating onto nutrient media, direct epifluorescence and electron microscopy examinations, detection of the elemental composition of cells, radiolabeling techniques, PCR and FISH methods. Of great importance is to ensure authenticity of microorganisms (if any in studied samples) and to standardize the protocols used to minimize a risk of external contamination. Although the convincing evidence of extraterrestrial microbial life will may come from the discovery of living cells in astromaterials, biomorphs and microfossils must also be regarded as a target in search of life evidence bearing in mind a scenario that alive microorganisms had not be preserved and underwent mineralization. Under the laboratory conditions, processes that accompanied fossilization of cyanobacteria were reconstructed, and artificially produced cyanobacterial stromatolites resembles by their morphological properties those found in natural Earth habitats. Regarding the vital importance of distinguishing between biogenic and abiogenic signatures and between living and fossil microorganisms in analyzed samples, it is worthwhile to use some previously developed approaches based on electron microscopy examinations and analysis of elemental composition of biomorphs in situ and comparison with the analogous data obtained for laboratory microbial cultures and fossilized microorganisms. This communication will be focused on the analysis of our experience in working with ancient microorganisms and fossils and discussion of some issues that are crucial for development of the program for future finding of extraterrestrial life and its evidence.

Abyzov, S. S.↗
NASA NTRS2005

A Methodology for the Design and Verification of Globally Asynchronous/Locally Synchronous Architectures

Recent advanced in model-checking have made it practical to formally verify the correctness of many complex synchronous systems (i.e., systems driven by a single clock). However, many computer systems are implemented by asynchronously composing several synchronous components, where each component has its own clock and these clocks are not synchronized. Formal verification of such Globally Asynchronous/Locally Synchronous (GA/LS) architectures is a much more difficult task. In this report, we describe a methodology for developing and reasoning about such systems. This approach allows a developer to start from an ideal system specification and refine it along two axes. Along one axis, the system can be refined one component at a time towards an implementation. Along the other axis, the behavior of the system can be relaxed to produce a more cost effective but still acceptable solution. We illustrate this process by applying it to the synchronization logic of a Dual Fight Guidance System, evolving the system from an ideal case in which the components do not fail and communicate synchronously to one in which the components can fail and communicate asynchronously. For each step, we show how the system requirements have to change if the system is to be implemented and prove that each implementation meets the revised system requirements through modelchecking.

Miller, Steven P.↗