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At least 1,171 records · Page 65

Small-scale plasma irregularities produced during electron attachment chemical releases

In situ measurements of small-scale plasma density irregularities made during sounding rocket experiments that released electron attachment materials into the ionosphere are presented. A 2D electrostatic simulation model that includes attachment chemistry is used to study the source and evolution of these irregularities. The simulation shows (1) that large electron flow velocity shears develop on the boundary of the electron depletion and (2) these shears drive a plasma instability that is the likely source of the irregularities.

Scales, W. A.↗

Biophotonics and Bone Biology

One of the more serious side effects of extended space flight is an accelerated bone loss. Rates of bone loss are highest in the weight-bearing bones of the hip and spine regions, and the average rate of bone loss as measured by bone mineral density measurements is around 1.2% per month for persons in a microgravity environment. It is well known that bone remodeling responds to mechanical forces. We are developing two-photon microscopy techniques to study bone tissue and bone cell cultures to better understand the fundamental response mechanism in bone remodeling. Osteoblast and osteoclast cell cultures are being studied, and the goal is to use molecular biology techniques in conjunction with Fluorescence Lifetime Imaging Microscopy (FLIM) to study the physiology of in-vitro cell cultures in response to various stimuli, such as fluid flow induced shear stress and mechanical stress. We have constructed a two-photon fluorescence microscope for these studies, and are currently incorporating FLIM detection. Current progress will be reviewed. This work is supported by the NASA John Glenn Biomedical Engineering Consortium.

Zimmerli, Gregory↗

Response Of Mineralizing And Non-Mineralizing Bone Cells To Fluid Flow: An In Vitro Model For Mechanotransruction

Humans reach peak bone mass at age 30. After this point, we lose 1 to 2 percent of bone mass each decade. In the microgravity environment of space, astronauts lose bone mass at an accelerated rate of 1 to 2 percent each month. When astronauts travel to Mars, they may be in space for as long as 3 years. During this time, they may lose about half of their bone mass from weight-bearing bones. This loss may be irreversible. The drastic loss in bone that astronauts experience in space makes them much more vulnerable to fractures. In addition, the corresponding removal of calcium from bone results in higher levels of calcium in the blood, which increases the risk of developing kidney stones. Currently, studies are being conducted which investigate factors governing bone adaptation and mechanotransduction. Bone is constantly adapting in response to mechanical stimuli. Increased mechanical loading stimulates bone formation and suppresses bone resorption. Reduction in mechanical loading caused by bedrest, disuse, or microgravity results in decreased bone formation and possibly increased bone resorption. Osteoblasts and osteoclasts are the two main cell types that participate in bone remodeling. Osteoblasts are anabolic (bone-forming) cells and osteoclasts are catabolic (bone-resorbing) cells. In microgravity, the activity of osteoblasts slows down and the activity of osteoclasts may speed up, causing a loss of bone density. Mechanotransduction, the molecular mechanism by which mechanical stimuli are converted to biochemical signals, is not yet understood. Exposure of cells to fluid flow imposes a shear stress on the cells. Several studies have shown that the shear stress that results from fluid flow induces a cellular response similar to that induced by mechanical loading. Thus, fluid flow can be used as an in vitro model to simulate the mechanical stress that bone cells experience in vivo. Previous in vitro studies have shown that fluid flow induces several responses in osteoblasts, including increased proliferation, osteoblastic differentiation, alkaline phosphatase activity, and production of nitric oxide, prostaglandins, and osteopontin. Several proteins have been implicated in osteoblastic mechanotransduction including Bone Morphogenetic Protein-2 (BMP-2), parathyroid hormone, 1,25-dihydroxyvitamin D3 receptor, osteopontin (OPN), osteoprotegerin (OPG), and alkaline phosphatase (AP). We will characterize relative levels of each protein in mineralizing or non-mineralizing MC3T3 osteoblastic cells that have been exposed to fluid flow compared to non-fluid flow using immunofluorescent staining and two- photon laser microscopy as well as western blotting. Because calcium-mediated pathways are important in osteoblastic signaling, we will transfect MC3T3 cells with cameleon probes for Ca2+ containing YFP and CFP. Results will be analyzed using FRET/FLIM to study differential release of intracellular Ca(2+) in response to fluid flow and conditions inducing matrix mineralization. In addition, we plan to conduct several microarray experiments to determine differential gene expression in MC3T3 cells in response to fluid flow and conditions inducing mineralization.

Makuch, Lauren A.↗

Microfluidics, Chromatography, and Atomic-Force Microscopy

A Raman-and-atomic-force microscope (RAFM) has been shown to be capable of performing several liquid-transfer and sensory functions essential for the operation of a microfluidic laboratory on a chip that would be used to perform rapid, sensitive chromatographic and spectro-chemical analyses of unprecedentedly small quantities of liquids. The most novel aspect of this development lies in the exploitation of capillary and shear effects at the atomic-force-microscope (AFM) tip to produce shear-driven flow of liquids along open microchannels of a microfluidic device. The RAFM can also be used to perform such functions as imaging liquids in microchannels; removing liquid samples from channels for very sensitive, tip-localized spectrochemical analyses; measuring a quantity of liquid adhering to the tip; and dip-pen deposition from a chromatographic device. A commercial Raman-spectroscopy system and a commercial AFM were integrated to make the RAFM so as to be able to perform simultaneous topographical AFM imaging and surface-enhanced Raman spectroscopy (SERS) at the AFM tip. The Raman-spectroscopy system includes a Raman microprobe attached to an optical microscope, the translation stage of which is modified to accommodate the AFM head. The Raman laser excitation beam, which is aimed at the AFM tip, has a wavelength of 785 nm and a diameter of about 5 m, and its power is adjustable up to 10 mW. The AFM is coated with gold to enable tip-localized SERS.

Anderson, Mark↗

SAPS-Associated Explosive Brightening on the Duskside: A New Type of Onset- Like Disturbance

Quasi-periodic energetic particle injections have been observed at geosynchronous orbit on the dusk-side during a steady magnetospheric convection event. We examine high-resolution auroral imager data and ground magnetometer data associated with the first of these injections and conclude that it was not associated with classical sub-storm signatures. It is proposed that these injections are caused by the explosive nonlinear growth of a shear flow-ballooning instability in the region where sub-auroral polarization streams (SAPS) also occur. It is suggested that interchange will occur preferentially in the low-conductivity SAPS region since the magnetic Richardson number is lowest there and the "line-tying" effect will also be least stabilizing there. We propose that the observed particle injection signatures and auroral morphology constitute a new type of SAPS-associated explosive "onset-like" disturbance that can occur during intervals of strong convection.

Henderson, M. G.↗

Transitional mixing in a binary species supercritical, temporal mixing layer

The turbulent mixing of fluids at high pressure is a topic of much interest as it is relavant both to the natural phenomena and to technical applications. In the realm of technical applications, liquid rocket combustion presents a particular challenge as the operating conditions are supercritical with respect to both fuel and oxidizer, making mandatory the understanding of supercritical fluid behavior for a potentially explosive mixture. In this situation, numerical simulations with validated models can contribute information that would be otherwise impossible to obtain.

supercritical↗

Three-dimensional simulation study of ionospheric plasma clouds

The results of fully three-dimensional numerical simulations of ionospheric plasma cloud evolution are presented. The evolution of the plasma cloud considered by Drake and Huba (1987) in the limit of vanishingly small ion compressibility is discussed. Simulations support the results of the analytical theory: finite plasma temperature, combined with fully three-dimensional plasma dynamics, is a stabilizing influence on plasma cloud evolution. This stability is associated with sheared azimuthal ion flows in the vicinity of the cloud surface. Cloud evolution using realistic values of ion compressibility show that the cloud rapidly diffuses to a state in which the sheared azimuthal flow is substantially reduced; subsequently, the cloud becomes unstable and structures.

Zalesak, S. T.↗

Self-Diffusion of Drops in a Dilute Sheared Emulsion

Self-diffusion coefficients that describe cross-flow migration of non-Brownian drops in a dilute sheared emulsion were obtained by trajectory calculations. A boundary integral formulation was used to describe pairwise interactions between deformable drops; interactions between undeformed drops were described with mobility functions for spherical drops. The results indicate that drops have large anisotropic self-diffusivities which depend strongly on the drop viscosity and modestly on the shear-rate. Pairwise interactions between drops in shear-flow do not appreciably promote drop breakup.

Loewenberg, Michael↗

Experimental Reacting Hydrogen Shear Layer Data at High Subsonic Mach Number

The flow in a planar shear layer of hydrogen reacting with hot air was measured with a two-component laser Doppler velocimeter (LDV) system, a schlieren system, and OH fluorescence imaging. It was compared with a similar air-to-air case without combustion. The high-speed stream's flow speed was about 390 m/s, or Mach 0.71, and the flow speed ratio was 0.34. The results showed that a shear layer with reaction grows faster than one without; both cases are within the range of data scatter presented by the established data base. The coupling between the streamwise and the cross-stream turbulence components inside the shear layers was low, and reaction only increased it slightly. However, the shear layer shifted laterally into the lower speed fuel stream, and a more organized pattern of Reynolds stress was present in the reaction shear layer, likely as a result of the formation of a larger scale structure associated with shear layer corrugation from heat release. Dynamic pressure measurements suggest that coherent flow perturbations existed inside the shear layer and that this flow became more chaotic as the flow advected downstream. Velocity and thermal variable values are listed in this report for a computational fluid dynamics (CFD) benchmark.

Chang, C. T.↗

Digital Particle Image Velocimetry (DPIV) Used for Space-Time Correlations in Nozzle Flow

An optical measurement technique known as Digital Particle Image Velocimetry (DPIV) was used previously to characterize the first- and second-order statistical properties of both cold and hot jet flows from externally mixed nozzles in NASA Glenn Research Center's Nozzle Acoustic Test Rig. In this technique, an electronic camera records particles entrained in a flow as a laser light sheet is pulsed at two instances in time. Correlation processing of the recorded particle image pairs yields the two-component velocity field across the imaged plane of the flow. The information acquired using DPIV is being used to improve our understanding of the decay of turbulence in jet flows-a critical element for understanding the acoustic properties of the flow. Recently, two independent DPIV systems were installed in Glenn's Small Hot Jet Acoustic Rig, enabling multiplane correlations in time and space. The data were collected over a range of different Mach numbers and temperature ratios. DPIV system 1 was fixed to a large traverse rig, and DPIV system 2 was mounted on a small traverse system mounted on the large traverse frame. The light sheets from the two DPIV systems were aligned to lie in the same axial plane, with DPIV system 2 being independently traversed downstream along the flow direction. For each measurement condition, the DPIV systems were started at a fully overlapping orientation. A polarization separation technique was used to avoid cross-talk between the two systems. Then, the DPIV systems fields were shifted axially apart, in successively increasing steps. The downstream DPIV system 2 was triggered at a short time delay after the upstream DPIV system 1, where the time delay was proportional to the convective flow velocity in the shear layer of the jet flow and the axial separation of the two DPIV systems. The acquired data were processed to obtain the instantaneous velocity vector maps over a range of time delays and spatial separations. The velocity fields from the different DPIV systems were then cross-correlated to determine the degree of correlation remaining in the flow as the downstream convection distance was increased. The new data provide Lagrangian measurements of the convective turbulent structures in the shear layer of an exhaust nozzle. These measurements, obtained in both cold and hot flows, will be used to validate and correct models for space-time velocity correlations-long a missing key to predicting jet noise.

Wernet, Mark P.↗

A New K-epsilon Eddy Viscosity Model for High Reynolds Number Turbulent Flows: Model Development and Validation

A new k-epsilon eddy viscosity model, which consists of a new model dissipation rate equation and a new realizable eddy viscosity formulation, is proposed. The new model dissipation rate equation is based on the dynamic equation of the mean-square vorticity fluctuation at large turbulent Reynolds number. The new eddy viscosity formulation is based on the realizability constraints: the positivity of normal Reynolds stresses and Schwarz' inequality for turbulent shear stresses. We find that the present model with a set of unified model coefficients can perform well for a variety of flows. The flows that are examined include: (1) rotating homogeneous shear flows; (2) boundary-free shear flows including a mixing layer, planar and round jets; (3) a channel flow, and flat plate boundary layers with and without a pressure gradient; and (4) backward facing step separated flows. The model predictions are compared with available experimental data. The results from the standard k-epsilon eddy viscosity model are also included for comparison. It is shown that the present model is a significant improvement over the standard k-epsilon eddy viscosity model.

Shih, T.-H.↗

Comparison of coordinate-invariant and coordinate-aligned upwinding for the Euler equations

A floating-shock fitting method for the Euler equations has been developed that uses one-sided spatial differences along and across streamlines. The coordinate-invariant formulation of the spatial differences permits automatic capture of shears. Results are presented for unsteady shocked flow in a duct with a ramp, for supercritical flow over a circular cylinder, and for subsonic, transonic, and supersonic (0.3 is less than or equal to M(sub infinity) is less than 1.5) flow over airfoils. In flows with strong shears, the coordinate-invariant differencing concept appears to yield some gains in accuracy over Euler methods that rely on coordinate-aligned differencing concepts. In applications to transonic airfoils, fitted shocks have a tendency to be predicted upstream of captured shocks, regardless of whether coordinate-invariant or coordinate-aligned differencing is used. The coordinate-invariant differencing method requires between 2 and 3.5 times as much computing time as its coordinate-aligned counterpart.

Hartwich, Peter M.↗

Shock turbulence interaction in the presence of mean shear - An application of rapid distortion theory

The present examination of the response of anisotropic turbulent flows to a shock wave uses incompressible, homogeneous rapid distortion theory and idealizes the shock-wave as 1D compression. Attention is given to the shock's effect on axisymmetric flow and both the normal and oblique cases for shear flow. The oblique angle between the directions of compression and shear significantly affects turbulence evolution. Compression amplifies all components of turbulence intensity.

Mahesh, Krishnan↗

Critical Shear Stress for Erosion Under Laminar Jet Flow

Particle erosion is an important process in both natural and manmade environments. For example, in natural environments particle or sediment erosion rates determine the course of rivers, depth of streams, and fate of deltas. The interplay between erosion rates and flow patterns can have dramatic impact on shorelines. Where hydrodynamic jet flows or rip currents change direction, sediments may accumulate or disperse altering the shape of coastlines and the ecosystems and economies that rely on them. In man-made systems, jet flows may be used to mobilize particles from surfaces or within industrial scale mixing tanks. For example, in the nuclear industry, impinging jet mixers may be used to mobilize and suspend sludges at the bottom of waste tanks. In each of these examples, the Shields diagram is useful to determine the conditions under which erosion occurs. This diagram plots the scaled critical shear stress for erosion as a function of the particle size as embedded in particle Reynolds numbers, Archimedes numbers, or their equivalents. Although the data, that carries a large spread, is largely in agreement with mathematical models, for the smallest particles, model predictions diverge from each other and from experimental data. Indeed, there is substantial uncertainty about the Shields diagrams at relatively small particle sizes and flow rates. Here we evaluate critical shear stresses for erosion under laminar jet flow. To date critical shear stresses for erosion, as embodied in Shields diagrams, have largely been developed for turbulent flow both experimental and mathematically. However, curves on the Shields diagrams diverge among various models at lower particle Reynolds numbers. Indeed, how critical shear stresses for erosion develop under fully laminar jet flow conditions (not simply laminar boundary layers under turbulent flow) remains unclear. Here we address this gap, which is particularly important for mobilization of the smallest particles. We find the Shields parameter to be inversely proportional to the particle Reynolds numbers under laminar jet flow conditions similar to turbulent conditions.

critical shear stresses, erosion rates, effective ↗

Mechanisms for the Crystallization of ZBLAN

A number of research teams have observed that glass forming melts that are solidified in low-g exhibit enhanced glass formation. This project will examine one of these glasses, the heavy metal fluoride glass ZBLAN. A four year ground based research program has been approved to examine the crystallization of ZBLAN glasses with the purpose of testing a theory for the crystallization of ZBLAN glass. The theory could explain the general observations of enhanced glass formation of other glasses melted and solidified in low-g. Fluid flow in 1-g results from buoyancy forces and surface tension driven convection. This fluid flow can introduce shear in undercooled liquids in 1-g. In low-g it is known that fluid flows are greatly reduced so that the shear rate in fluids in low-g are extremely low. It is believed that fluids may have some weak structure in the absence of flow. Even very small shear rates could cause this structure to collapse in response to the shear. A general result would be shear thinning of the fluid. The hypothesis of this research is that: Shear thinning in undercooled liquids increases the rate of nucleation and crystallization of glass forming melts. Shear of the melt can be reduced in low-g enhancing undercooling and glass formation. Samples will be melted and quenched in 1-g under quiescent conditions at a number of controlled cooling rates to determine times and temperatures of crystallization and heated at controlled heating rates to determine kinetic crystallization parameters. Experiments will also be performed on the materials while under controlled vibration conditions and compared with the quiescent experiments in order to evaluate the effect of shear in the liquid on crystallization kinetics. After the experimental parameters are well known, experiments will be repeated under low-g (and 2-g) conditions on the KC-135 aircraft during low-g parabolic maneuvers. The results will determine the effects of shear on crystallization. Our experimental setups will be designed with low-g experiments in mind and will be tested as breadboard low-g experiments. It is very likely that the thermal analysis instrumentation can be adapted to be run in the microgravity glovebox facilities. Critical space experiments may result to test the theory at longer low-g time experiments in space.

Ethridge, Edwin C.↗

Flow in the Proximity of the Pin-Tool in Friction Stir Welding and Its Relation to Weld Homogeneity

In the Friction Stir Welding (FSW) process a rotating pin inserted into a seam literally stirs the metal from each side of the seam together. It is proposed that the flow in the vicinity of the pin-tool comprises a primary rapid shear over a cylindrical envelope covering the pin-tool and a relatively slow secondary flow taking the form of a ring vortex about the tool circumference. This model is consistent with a plastic characterization of metal flow, where discontinuities in shear flow are allowed but not viscous effects. It is consistent with experiments employing several different kinds of tracer: atomic markers, shot, and wire. If a rotating disc with angular velocity w is superposed on a translating continuum with linear velocity omega, the trajectories of tracer points become circular arcs centered upon a point displaced laterally a distance v/omega from the center of rotation of the disc in the direction of the advancing side of the disc. In the present model a stream of metal approaching the tool (taken as the coordinate system of observation) is sheared at the slip surface, rapidly rotated around the tool, sheared again on the opposite side of the tool, and deposited in the wake of the tool. Local shearing rates are high, comparable to metal cutting in this model. The flow patterns in the vicinity of the pin-tool determine the level of homogenization and dispersal of contaminants that occurs in the FSW process. The approaching metal streams enfold one another as they are rotated around the tool. Neglecting mixing they return to the same lateral position in the wake of the tool preserving lateral tracer positions as if the metal had flowed past the tool like an extrusion instead of being rotated around it. (The seam is, however, obliterated.) The metal stream of thickness approximately that of the tool diameter D is wiped past the tool at elevated temperatures drawn out to a thickness of v/2(omega) in the wiping zone. Mixing distances in the wiping zone are multiplied in the unfolded metal. Inhomogeneities on a smaller scale than the mixing length are obliterated, but structure on a larger scale may be transmitted to the wake of a FSW weld.

Nunes, Arthur C., Jr.↗

Mixed-flow design for microfluidic printing of two-component polymer semiconductor systems

The rational creation of two-component conjugated polymer systems with high levels of phase purity in each component is challenging but crucial for realizing printed soft-matter electronics. Here, we report a mixed-flow microfluidic printing (MFMP) approach for two-component π -polymer systems that significantly elevates phase purity in bulk-heterojunction solar cells and thin-film transistors. MFMP integrates laminar and extensional flows using a specially microstructured shear blade, designed with fluid flow simulation tools to tune the flow patterns and induce shear, stretch, and pushout effects. This optimizes polymer conformation and semiconducting blend order as assessed by atomic force microscopy (AFM), transmission electron microscopy (TEM), grazing incidence wide-angle X-ray scattering (GIWAXS), resonant soft X-ray scattering (R-SoXS), photovoltaic response, and field effect mobility. For printed all-polymer (poly[(5,6-difluoro-2-octyl-2H-benzotriazole-4,7-diyl)-2,5-thiophenediyl[4,8-bis[5-(2-hexyldecyl)-2-thienyl]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl]-2,5-thiophenediyl]) [J51]:(poly{[N,N′-bis(2-octyldodecyl)naphthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]-alt-5,5′-(2,2′-bithiophene)}) [N2200]) solar cells, this approach enhances short-circuit currents and fill factors, with power conversion efficiency increasing from 5.20% for conventional blade coating to 7.80% for MFMP. Moreover, the performance of mixed polymer ambipolar [poly(3-hexylthiophene-2,5-diyl) (P3HT):N2200] and semiconducting:insulating polymer unipolar (N2200:polystyrene) transistors is similarly enhanced, underscoring versatility for two-component π -polymer systems. Mixed-flow designs offer modalities for achieving high-performance organic optoelectronics via innovative printing methodologies.

42 ENGINEERING↗

What Supergranule Flow Models Tell Us About the Sun's Surface Shear Layer and Magnetic Flux Transport

Models of the photospheric flows due to supergranulation are generated using an evolving spectrum of vector spherical harmonics up to spherical harmonic wavenumber l~1500. Doppler velocity data generated from these models are compared to direct Doppler observations from SOHO/MDI and SDO/HMI. The models are adjusted to match the observed spatial power spectrum as well as the wavenumber dependence of the cell lifetimes, differential rotation velocities, meridional flow velocities, and relative strength of radial vs. horizontal flows. The equatorial rotation rate as a function of wavelength matches the rotation rate as a function of depth as determined by global helioseismology. This leads to the conclusions that the cellular structures are anchored at depths equal to their widths, that the surface shear layer extends to at least 70 degrees latitude, and that the poleward meridional flow decreases in amplitude and reverses direction at the base of the surface shear layer (approx.35 Mm below the surface). Using the modeled flows to passively transport magnetic flux indicates that the observed differential rotation and meridional flow of the magnetic elements are directly related to the differential rotation and meridional flow of the convective pattern itself. The magnetic elements are transported by the evolving boundaries of the supergranule pattern (where the convective flows converge) and are unaffected by the weaker flows associated with the differential rotation or meridional flow of the photospheric plasma.

Hathaway, David↗