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At least 1,171 records · Page 65

Coarsening Up: Expanding the Particle Size Distributions of Lunar Simulants to Encompass A Comprehensive Range of Regolith Granularity

Due to a paucity of lunar regolith samples, simulated granular materials (simulants) have been developed and deployed for a variety of engineering and research purposes in an attempt to meet the requirements for lunar exploration. Broadly speaking, these manufactured materials aim to replicate the compositional (i.e., mineralogy and chemistry) and physical (e.g., particle size distribution, particle shape) profiles of lunar soil samples collected during the Apollo missions. While creating a “near-perfect” lunar simulant is prohibitive due to the unique formative processes (i.e., meteorite impacts, solar wind implantation) and conditions (anhydrous, reducing environment and exposure to radiation on the surface over billions of years) encountered on the moon, certain granular properties of the regolith can be recreated with a high level of fidelity by leveraging contemporary technologies. A fundamental physical property and descriptor, grain size can be correlated between samples and replicated through processing; however, a holistic understanding of the particle size distribution of lunar regolith must be considered when creating analog materials. This study produces particle size distributions for Apollo 16 returned samples, incorporating the sieved out >1 cm coarse-grained fraction, in order to provide a more comprehensive characterization of surficial lunar regolith grain sizes for the development of highlands-type granular analog testing materials.

R. N. Kovtun↗

Quantifying the Ability of Magnetohydrodynamic Models to Reproduce Observed Birkeland Current and Auroral Electrojet Magnitudes

Although global magnetohydrodynamic (MHD) models have increased in sophistication and are now at the forefront of modeling Space Weather, there is still no clear understanding of how well these models replicate the observed ionospheric current systems. Without a full understanding and treatment of the ionospheric current systems, global models will have significant shortcomings that will limit their use. In this study we focus on reproducing observed seasonal interhemispheric asymmetry in ionospheric currents using the Space Weather Modeling Framework (SWMF). We find that SWMF does reproduce the linear relationship between the electrojets and the FACs, despite the underestimation of the currents’ magnitudes. Quantitatively, we find that at best SWMF is only capturing approximately 60% of the observed current. We also investigate how varying F10.7 effects the ionospheric potential and currents during the summer and winter. We find that simulations ran with higher F10.7 result in lower ionospheric potentials. Additionally, we find that the models do not always replicate the expected behavior of the currents with varying F10.7. This work points to a needed improvement in ionospheric conductance models.

Tre'Shunda James↗

Preliminary Parametric Cost Model for X-Ray Grazing Incidence Optics for Space Flight

Parametric cost models offer a lot of utility in planning and comparing different missions and concepts. Furthermore, they have a significant advantage over other estimating methodologies as they can be quickly and easily replicated. The cost model presented in this paper focuses on estimating the most likely cost for the X-ray Mirror Assembly (XMA) of space-based X-ray telescopes with grazing incidence optics. The authors recognize the complexities of generating a single cost model that covers multiple fabrication techniques, so they have limited this preliminary study to techniques used on previous and current missions, such as direct polished glass full shell, replicated, and foil optics

Space Telescope Cost Model↗

Spacecraft Priming Analysis and Research Test Apparatus: Number 1 (SPARTAN 1), Test and Analysis Results

Spacecraft with Earth-storable propellants are required by range safety to launch with propellant isolated from the thrusters for ground operations, which is often accomplished by placing a pyrotechnic, or a latching isolation valve between the tank and the thrusters. Once on-orbit, downstream lines are vented out to space through the thrusters and are left evacuated and at vacuum pressure. Propellant isolation valve then opens and floods the lines with high velocity propellant which can lead to a significant pressure surge upon filling. Replicating this event during ground testing using water as a propellant simulant has always been difficult and costly to accurately perform due to the low vacuum pressure targeted on the downstream side of the system. This report describes the Spacecraft Priming Analysis and Research Test Apparatus (SPARTA) at Marshall Space Flight Center’s (MSFC) Component Development Area (CDA) and the initial test series meant to be a pathfinder for construction methods and test procedures to reduce the cost of future testing. The test data collected are critical to analytical models as the details to replicate prior ground tests are often lacking in enough detail to adequately model the test that was conducted. This report also describes the Thermal Desktop/FloCAD analysis that was performed alongside the test series. The test methods defined for this campaign showed a dramatic improvement on heritage test procedures. Test pace increased due to faster drying and pulling vacuum more effectively. Results generated in this test matched analytical predictions very well and provide a releasable set of test data to be leveraged on future NASA and commercial partner spacecraft development programs.

spacecraft↗

Independent Scientific Review of Biological Assumptions and Planned Ultraviolet Treatment for Mars Sample Return Backward Planetary Protection

The Backward Planetary Protection Study Team (“the Study Team”), assembled by the Office of the Chief Scientist (OCS) at the request of the Office of Planetary Protection (OPP), has conducted numerous detailed discussions on the potential use of an active ultraviolet (UV) approach for minimizing or eliminating biological contamination of the exterior of the planned Orbiting Sample container (OS) of the Mars Sample Return (MSR) mission. This review of the backward planetary protection (BPP) considerations for the MSR mission focused on two key parameters that are opportunities for further process development to support implementation of planetary protection practices in meeting mission requirements. These parameters include: 1) Sub-cellular biological assumptions – should Mars missions consider self-replicating organisms only or do they need to consider non-self-replicating entities without a cellular organization such as viruses, prions, etc. and macromolecules capable of genetic information transfer, as well? 2) UV biocidal impact – what is the UV fluence in the Mars atmosphere and its associated potential biocidal credit? The assembled subject matter experts (SMEs) in the Study Team are listed in Appendix A, with a brief description of each member’s relevant expertise.

Mamta Patel Nagaraja↗

An Assessment of Turbulence Models for MSR Sample Retrieval Lander Aerothermal Design

Several cases from past entries in the T9 and T5 hypersonic test facilities are examined using various turbulence models and shock-layer thermochemistries with the objectives of verifying/validating turbulence models and determining appropriate multiplicative factors on predicted turbulent heating for use in aerothermal design of the heatshield of the Sample Retrieval Lander (SRL) of the Mars Sample Return (MSR) Flagship mission. The zero-equation turbulence models of Baldwin-Lomax and Cebeci-Smith are shown to replicate measurements to within experimental uncertainty for the T9 cases. For T5 cases, the zero-equation model of Baldwin-Lomax can also replicate measurements provided the shock-layer thermochemistry is modified. The modified thermochemistry resolves a dichotomy from past CFD that required different wall catalycity assumptions in the laminar and turbulent flow regimes. The present work shows that such assumptions are not necessary. Finally, an initial estimate for a multiplicative factor on turbulent heating is estimated for the SST turbulence model – the chosen model for SRL aerothermal design simulations.

Mars entry↗

Evaluation of Vertical Patterns in Chlorophyll-A Derived From A Data Assimilating Model of Satellite-Based Ocean Color

Satellite-based sensors of ocean color have become the primary tool to infer changes in surface chlorophyll, while BGC-Argo floats are now filling the information gap at depth. Here we use BGC-Argo data to assess depth-resolved information on chlorophyll-a derived from an ocean biogeochemical model constrained by the assimilation of surface ocean color remote sensing. The data-assimilating model replicates well the general seasonality and meridional gradients in surface and depth-resolved chlorophyll-a inferred from the float array in the Southern Ocean. On average, the model tends to overestimate float-based chlorophyll, particularly at times and locations of high productivity such as the beginning of the spring bloom, subtropical deep chlorophyll maxima, and non-iron limited regions of the Southern Ocean. The highest model RMSE in the upper 50 m with respect to the float array is of 0.6 mg Chl m −3 , which should allow the detection of seasonal changes in float-based biomass (varying between 0.01 and >1 mg Chl m −3 ) but might hinder the identification of subtle changes in chlorophyll at narrow local scales. Both model and float profiling data show good agreement with in situ data from station ALOHA, with model estimates showing a slight accuracy edge in inferring depth-resolved observations. Uncertainties in float bio-optical estimates impede their use as a reliable benchmark for validation, but the general qualitative agreement between model and float data provides confidence in the ability of model to replicate biogeochemical features below the surface, where data is not directly constrained by the assimilation of satellite ocean color.

Lionel A Quintero↗

Update on Planetary, Lunar, & Asteroid Natural Environment Testbed (PLANET)

At ISMSE15, the Planetary, Lunar, & Asteroid Natural Environment Testbed (PLANET) was introduced as an upcoming high-fidelity, combined-effects planetary surface environment laboratory. This year, we will present an update on the facility status, describing the procurement, installation, commissioning of the chamber in Huntsville, Alabama (USA). With NASA’s push to return to the Moon through the Artemis program, there is a clear need for more high-fidelity test chambers that can replicate multiple aspects of the lunar surface, especially the fine, dusty lunar soil known as regolith. The PLANET chamber is designed to fill this gap, enabling research & development, qualification, and verification testing in a combined lunar surface environment, at an affordable price, for government, commercial/industry, and academic partners. Features include a large regolith simulant bed, low energy electron and ion sources to replicate the solar wind, full-spectrum UV and Solar simulation, and a liquid nitrogen cryogenic shroud, all in a high-vacuum environment (as low as 10^-7 mbar). PLANET’s initial focus will be on the lunar environment, but other surface environments (Martian, asteroid, etc.) are also possible to simulate. Besides the environmental instrumentation, PLANET will be equipped with specialized test systems that the Space Environmental Effects Team has developed over the past two years, including an in-situ tribometer and uniform dust distribution system. The chamber is currently being manufactured, with plans to install in May 2024. This will be followed by outfitting and commissioning. The challenges and accomplishments seen during this process will be detailed, and data from the first tests performed in PLANET will be shared with the community.

environmental testing↗

Open Science Approach to Analyze Climate-Crop Relationships in the US Leveraging GES DISC and Galaxy Workflows

Understanding the intricate relationship between climate variability and agricultural production is crucial for ensuring food security. This study investigates the impact of climate parameters, such as temperature, precipitation, and soil moisture, on major US crop yields. Adopting an open science approach, the study analyzes the impact of climate on agricultural production in the United States. The Galaxy workflow engine serves as the primary tool for integrating climate data from the Goddard Earth Sciences Data and Information Services Center (GES DISC), retrieved via the Giovanni system, with yield statistics from the United States Department of Agriculture’s National Agricultural Statistics Service (USDA NASS). Extensions for reading, preprocessing, and analyzing external data have been developed, enabling the creation of workflows within the Galaxy platform. The development of a reproducible workflow allows for the calculation of seasonal climate averages, which are then assessed for their correlation with crop yields. This methodology ensures the replicability of the research, promoting transparency and collaboration in the scientific community. Correlational and regression analyses have been applied to different sub-zones and crops. The findings from this research offer valuable insights into the relationship between climate parameters and crop yields. These insights contribute to a deeper understanding of climate-crop relationships, providing a solid foundation for informed decision-making in the agricultural sector. The high correlation values indicate a significant relationship between climate parameters and crop yields, underscoring the importance of considering climate factors in agricultural planning and policymaking. This research also exemplifies the power of open science in advancing our understanding of complex environmental and agricultural phenomena. By leveraging open data and services, it provides a robust and replicable framework for future studies in this critical field.

Open science↗

Pyrococcus furiosus strains and methods of using same

Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

59 BASIC BIOLOGICAL SCIENCES↗

Particle Communication and Domain Neighbor Coupling: Scalable Domain Decomposed Algorithms for Monte Carlo Particle Transport

In order to run Monte Carlo particle transport calculations on new supercomputers with hundreds of thousands or millions of processors, care must be taken to implement scalable algorithms. This means that the algorithms must continue to perform well as the processor count increases. In this paper, we examine the scalability of:(1) globally resolving the particle locations on the correct processor, (2) deciding that particle streaming communication has finished, and (3) efficiently coupling neighbor domains together with different replication levels. We have run domain decomposed Monte Carlo particle transport on up to 2 21 = 2,097,152 MPI processes on the IBM BG/Q Sequoia supercomputer and observed scalable results that agree with our theoretical predictions. These calculations were carefully constructed to have the same amount of work on every processor, i.e. the calculation is already load balanced. We also examine load imbalanced calculations where each domain’s replication level is proportional to its particle workload. In this case we show how to efficiently couple together adjacent domains to maintain within workgroup load balance and minimize memory usage.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Rapid and highly fieldable viral diagnostic

The present invention relates to a rapid, highly fieldable, nearly reagentless diagnostic to identify active RNA viral replication in a live, infected cells, and more particularly in leukocytes and tissue samples (including biopsies and nasal swabs) using an array of a plurality of vertically-aligned nanostructures that impale the cells and introduce a DNA reporter construct that is expressed and amplified in the presence of active viral replication.

59 BASIC BIOLOGICAL SCIENCES↗

Method and apparatus for reducing memory access latency

Logic such as a memory controller writes primary data from an incoming write request as well as corresponding replicated primary data (which is a copy of the primary data) to one or more different memory banks of random access memory in response to determining a memory access contention condition for the address (including a range of addresses) corresponding to the incoming write request. When the memory bank containing the primary data is busy servicing a write request, such as to another row of memory in the bank, a read request for the primary data is serviced by reading the replicated primary data from the different memory bank of the random access memory to service the incoming read request.

97 MATHEMATICS AND COMPUTING↗

Investigating YAP’s role in Human Mammary Epithelial Cell (HMEC) Immortalization and Cancer Stem Cell (CSC) Phenotype Acquisition during Early Breast Cancer Progression

The malignant aspect of tumorigenesis in breast cancer is initiated when human mammary epithelial cells (HMEC) first immortalize (gain the ability to proliferate indefinitely) and subsequently undergo oncogenic activation. Our laboratory has developed a two-step HMEC immortalization process that we believe accurately models early breast cancer progression. Normal finite pre-stasis HMEC must overcome two distinct senescence barriers to achieve full immortality: stasis (stress-associated senescence) and replicative senescence, respectively. HMEC bypass stasis, and become post-stasis, when errors in the retinoblastoma pathway are present. Replicative senescence, a product of continuously shortening telomeres producing telomere dysfunction, is overcome by the reactivation of telomerase. Our lab has called HMEC that acquire the error allowing telomerase expression conditionally immortal (CI); CI cells may still have low telomerase expression. Full immortality is achieved when sufficient telomerase expression exists to maintain short stable telomeres following a process we have called conversion. The transcriptional coactivator YAP, upregulated in several human cancers, is closely correlated with a variety of oncogenic effects including proliferation and induction of cancer stem cell (CSC) properties. Our preliminary data have shown that YAP-transduced post-stasis HMEC expressed properties of CI cells. Growth curves indicated that some cell lines achieved full immortality; however, these cells had acquired an additional error – amplified expression of c-Myc, an oncogene known to induce telomerase in post-stasis or CI cells. The absence of uniform immortalization in the YAP-transduced cells suggests that YAP causes post-stasis HMEC to become conditionally immortal. This is further supported given that YAP-transduced post-stasis HMEC have decreased levels of the lncRNA MORT and rapidly go through conversion when p53 function is abrogated, well-documented attributes of CI HMEC. Previous studies have also reported that YAP controls the differentiation of breast progenitor cells; as a result, it is likely that YAP plays a role in inducing CSC phenotypes. In this study, we attempt to gain a greater understanding of YAP’s role in HMEC immortalization and induction of CSC properties in early breast cancer progression. We measured gene expression levels and attempted to identify proteins indicative of a CSC phenotype in YAP-transduced post-stasis HMEC. Our initial results did not reveal an association between YAP and HMEC conditional immortality or induction of CSC attributes in the cultures examined. However, using a YAP construct that promotes nuclear localization (where YAP is most active) has led to a potentially promising YAP-derived cell line that we presume to be immortal. We go on to demonstrate that YAP may be acting via non-transcriptional oncogenic roles in our HMEC specimens. Thus, we recommend future studies into YAP and the HMEC immortalization process given that they are promising targets for breast cancer prevention therapies.

Shah, Manav↗

Development and Validation of a Simulation Testbed for the Intelligent Building Agents Laboratory (IBAL) Using TRNSYS

This paper documents the development and validation of a dynamic primary cooling and thermal storage system simulation testbed. The system simulation testbed, sIBAL, is based on the Intelligent Building Agents Laboratory (IBAL) at the National Institute of Standards and Technology (NIST), which is a research infrastructure and testbed for the development, evaluation, and demonstration of intelligent control algorithms. The sIBAL testbed developed in this project will serve as a virtual twin of the real facility for future control algorithm development. The details of the methodologies used to develop and validate the simulation testbed, which replicates the dynamic behaviors of the primary cooling and ice storage system in the IBAL facility, are presented. The simulation testbed was developed in TRNSYS using built-in component models and MATLAB functions to replicate the two water-cooled chillers, a thermal storage tank, pumps, valves and other components for four different operation modes. Experiments on IBAL components were designed and executed to generate experimental data for model development and verification of the simulation platform. The validation of the simulation results was carried out in two phases: 1) independent component simulations for the chillers and thermal storage tank, and 2) a combined testbed simulation of the entire hydronic system. Comparison of simulation results to the experimental data obtained from the IBAL facility showed errors within 1 °C for the temperatures outputs of both the chiller and the thermal storage model. The error is within an acceptable range for further intelligent control algorithms development. The findings from the study are summarized and presented along with areas where additional research is needed. In addition, data filtering procedures and model refinement measures utilized to improve the accuracy and accelerate the computation time of the simulation are presented.

cooling systems↗

GANISP: A GAN-Assisted Importance Splitting Probability Estimator

Designing manufacturing processes with high yield and strong reliability relies on effective methods for rare event estimation. Genealogical importance splitting reduces the variance of rare event probability estimators by iteratively selecting and replicating realizations that are headed towards a rare event. The replication step is difficult when applied to deterministic systems where the initial conditions of the offspring realizations need to be modified. Typically, a random perturbation is applied to the offspring to differentiate their trajectory from the parent realization. However, this random perturbation strategy may be effective for some systems while failing for others, preventing variance reduction in the probability estimate. This work seeks to address this limitation using a generative model such as a Generative Adversarial Network (GAN) to generate perturbations that are consistent with the attractor of the dynamical system. The proposed GAN-assisted Importance SPlitting method (GANISP) improves the variance reduction for the system targeted. An implementation of the method is available in a companion repository (https://github.com/NREL/GANISP).

generative adversarial network↗

GANISP: A GAN-Assisted Importance Splitting Probability Estimator: Preprint

To reduce the variance of rare event probability estimator, genealogical importance splitting marches towards a rare event by iteratively selecting and replicating realizations that are headed towards a rare event. The replication step is made difficult when applied to deterministic systems as the initial conditions of the offspring realizations need to be modified. Typically, a random perturbation is applied to the offspring to differentiate their trajectory from the parent realization. It is shown that a random perturbation strategy may be effective for some systems but may also fail for others, thereby preventing variance reduction in the probability estimate. To address this limitation, it is proposed to use a generative model such as a Generative Adversarial Network (GAN) to generate perturbations that are consistent with the attractor of the dynamical system. The GAN-assisted Importance SPlitting method (GANISP) improves the variance reduction for the system targeted. An implementation of the method is available in a companion repository (https://github.com/NREL/GANISP).

generative adversarial network↗

If One GEB is Good, a Community of GEBs is Better

Energy efficient, connected, grid-interactive, smart and flexible buildings are key to decarbonization, lowering energy use and improving the nation’s electricity grid. The U.S. Department of Energy’s Connected Communities initiative works to demonstrate how coordinated groups of highly efficient buildings combined with other distributed energy resources (DERs), such as electric vehicle (EV) charging, batteries, storage, demand response and photovoltaic (PV) generation can reliably and cost-effectively serve as grid assets by strategically deploying efficiency and demand flexibility while reducing carbon emissions. In 2021, DOE competitively awarded $61 million to a diverse portfolio of 10 pilot projects to promote grid-interactive efficient buildings (GEBs) working together to reliably and cost-effectively serve as grid assets while decarbonizing. Two of the main tenets of the program are measuring the communities’ energy and carbon performance and understanding how to replicate project successes in other communities. This paper begins with a discussion of what Connected Communities are (including a brief history) and their many benefits, including reduced carbon emissions and increased building efficiency and demand flexibility. Next, it provides an overview of the 10 projects, highlighting the diversity of approaches to measure success and replicate the projects: geographic locations; building types; utility, regulatory, market environments; and building vintages that will be used to test the ability of buildings to serve as grid resources. It concludes with a discussion of anticipated project impacts and the metrics that will be used to evaluate the Connected Communities projects.

Nemtzow, David↗