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At least 1,171 records · Page 65

Tailored approach to study Legionella infection using a lattice light sheet microscope (LLSM)

Legionella is a genus of ubiquitous environmental pathogens found in freshwater systems, moist soil, and composted materials. More than four decades of Legionella research has provided important insights into Legionella pathogenesis. Although standard commercial microscopes have led to significant advances in understanding Legionella pathogenesis, great potential exists in the deployment of more advanced imaging techniques to provide additional insights. The lattice light sheet microscope (LLSM) is a recently developed microscope for 4D live cell imaging with high resolution and minimum photo-damage. We built a LLSM with an improved version for the optical layout with two path-stretching mirror sets and a novel reconfigurable galvanometer scanner ( RGS ) module to improve the reproducibility and reliability of the alignment and maintenance of the LLSM. We commissioned this LLSM to study Legionella pneumophila infection with a tailored workflow designed over instrumentation, experiments, and data processing methods. Our results indicate that Legionella pneumophila infection is correlated with a series of morphological signatures such as smoothness, migration pattern and polarity both statistically and dynamically. Our work demonstrates the benefits of using LLSM for studying long-term questions in bacterial infection. Our free-for-use modifications and workflow designs on the use of LLSM system contributes to the adoption and promotion of the state-of-the-art LLSM technology for both academic and commercial applications.

59 BASIC BIOLOGICAL SCIENCES↗

Measurement Instruments

International Technology Corporation (INTEC) manufactures a line of instruments used for determining the optical properties of materials used in solar energy applications. Some of INTEC's instruments are based on NASA technology, including the Willey Alpha Meter and McDonald Emissometer. The emissometer is a commercial version of a system developed by Lewis Research Center to test efficiency- increasing coatings on solar panels. These instruments are used by manufacturers of solar equipment, government, university and private laboratories.

Source record↗

Design Evolution of the Wide Field Infrared Survey Telescope using Astrophysics Focused Telescope Assets (WFIRST-AFTA) and Lessons Learned

The design of the Wide Field Infrared Survey Telescope using Astrophysics Focused Telescope Assets (WFIRST-AFTA) continues to evolve as each design cycle is analyzed. In 2012, two Hubble sized (2.4 m diameter) telescopes were donated to NASA from elsewhere in the Federal Government. NASA began investigating potential uses for these telescopes and identified WFIRST as a mission to benefit from these assets. With an updated, deeper, and sharper field of view than previous design iterations with a smaller telescope, the optical designs of the WFIRST instruments were updated and the mechanical and thermal designs evolved around the new optical layout. Beginning with Design Cycle 3, significant analysis efforts yielded a design and model that could be evaluated for Structural-Thermal-Optical-Performance (STOP) purposes for the Wide Field Imager (WFI) and provided the basis for evaluating the high level observatory requirements. Development of the Cycle 3 thermal model provided some valuable analysis lessons learned and established best practices for future design cycles. However, the Cycle 3 design did include some major liens and evolving requirements which were addressed in the Cycle 4 Design. Some of the design changes are driven by requirements changes, while others are optimizations or solutions to liens from previous cycles. Again in Cycle 4, STOP analysis was performed and further insights into the overall design were gained leading to the Cycle 5 design effort currently underway. This paper seeks to capture the thermal design evolution, with focus on major design drivers, key decisions and their rationale, and lessons learned as the design evolved.

Thermal Design↗

Design Evolution of the Wide Field Infrared Survey Telescope Using Astrophysics Focused Telescope Assets (WFIRST-AFTA) and Lessons Learned

The design of the Wide Field Infrared Survey Telescope using Astrophysics Focused Telescope Assets (WFIRST-AFTA) continues to evolve as each design cycle is analyzed. In 2012, two Hubble sized (2.4 m diameter) telescopes were donated to NASA from elsewhere in the Federal Government. NASA began investigating potential uses for these telescopes and identified WFIRST as a mission to benefit from these assets. With an updated, deeper, and sharper field of view than previous design iterations with a smaller telescope, the optical designs of the WFIRST instruments were updated and the mechanical and thermal designs evolved around the new optical layout. Beginning with Design Cycle 3, significant analysis efforts yielded a design and model that could be evaluated for Structural-Thermal-Optical-Performance (STOP) purposes for the Wide Field Imager (WFI) and provided the basis for evaluating the high level observatory requirements. Development of the Cycle 3 thermal model provided some valuable analysis lessons learned and established best practices for future design cycles. However, the Cycle 3 design did include some major liens and evolving requirements which were addressed in the Cycle 4 Design. Some of the design changes are driven by requirements changes, while others are optimizations or solutions to liens from previous cycles. Again in Cycle 4, STOP analysis was performed and further insights into the overall design were gained leading to the Cycle 5 design effort currently underway. This paper seeks to capture the thermal design evolution, with focus on major design drivers, key decisions and their rationale, and lessons learned as the design evolved.

WFIRST↗

Cryogenic Optical Position Encoders for Mechanisms in the JWST Optical Telescope Element Simulator (OSIM)

The JWST Optical Telescope Element Simulator (OSIM) is a configurable, cryogenic, optical stimulus for high fidelity ground characterization and calibration of JWST's flight instruments. OSIM and its associated Beam Image Analyzer (BIA) contain several ultra-precise, cryogenic mechanisms that enable OSIM to project point sources into the instruments according to the same optical prescription as the flight telescope images stars - correct in focal surface position and chief ray angle. OSIM's and BIA's fifteen axes of mechanisms navigate according to redundant, cryogenic, absolute, optical encoders - 32 in all operating at or below 100 K. OSIM's encoder subsystem, the engineering challenges met in its development, and the encoders' sub-micron and sub-arcsecond performance are discussed.

Leviton, Douglas B.↗

Fabrication of MgF2 and LiF windows for the Hubble Space Telescope Imaging Spectrograph

Two prototype test windows (MgF2 and LiF) to be used on the 75-mm UV MAMA detector tubes for the Hubble Space Telescope Imaging Spectrograph are described. The spatial and optical constraints of this instrument dictate that the thickness of the window materials be no greater than 2-3 mm to achieve a minimum 50-percent transmission at hydrogen Lyman alpha (121.6 nm), and that the window must be domed to minimize optical aberrations and provide structural strength. The detector window has an input diameter of about 100 mm with a radius-of-curvature of 70 mm. The manufacturing processes involved in the fabrication of these windows is discussed, as well as test programs (optical and structural) to be performed at Goddard Space Flight Center.

Gormley, Daphne↗

Loading, electromyograph, and motion during exercise

A system is being developed to gather kineto-dynamic data for a study to determine the load vectors applied to bone during exercise on equipment similar to that used in space. This information will quantify bone loading for exercise countermeasures development. Decreased muscle loading and external loading of bone during weightlessness results in cancellous bone loss of 1 percent per month in the lower extremities and 2 percent per month in the calcaneous. It is hypothesized that loading bone appropriately during exercise may prevent the bone loss. The system consists of an ergometer instrumented to provide position of the pedal (foot), pedaling forces on the foot (on the sagittal plane), and force on the seat. Accelerometers attached to the limbs will provide acceleration. These data will be used as input to an analytical model of the limb to determine forces on the bones and on groups of muscles. EMG signals from activity in the muscles will also be used in conjunction with the equations of mechanics of motion to be able to discern forces exerted by specific muscles. The tasks to be carried out include: design of various mechanical components to mount transducers, specification of mechanical components, specification of position transducers, development of a scheme to control the data acquisition instruments (TEAC recorder and optical encoder board), development of a dynamic model of the limbs in motion, and development of an overall scheme for data collection analysis and presentation. At the present time, all the hardware components of the system are operational, except for a computer board to gather position data from the pedals and crank. This board, however, may be put to use by anyone with background in computer based instrumentation. The software components are not all done. Software to transfer data recorded from the EMG measurements is operational, software to drive the optical encoder card is mostly done. The equations to model the kinematics and dynamics of motion of the limbs have been developed, but they have not yet been implemented in software. Aside from the development of the hardware and software components of the system, the methodology to use accelerometers and encoders and the formulation of the appropriate equations are an important contribution to the area of biomechanics, particularly in space applications.

Figueroa, Fernando↗

Straylight comparison of SIRTF designs with different aperture stop locations

The APART/PADE program is used to examine whether the SIRTF aperture stop can be moved back to the primary mirror without jeopardizing the requirement for natural background-limited observations. The straylight performance of the baseline design, which has the aperture stop at the secondary mirror, is compared to that of a revised design with the stop at the primary mirror. Both designs include reimaging optics and a LYOT stop, as well as a chopping secondary mirror. The straylight due to off-axis sources and telescope thermal emission is investigated at 50 and 550 microns. It is found that in SIRTF, where every instrument will include reimaging optics and a LYOT stop, the aperture stop may be moved to the primary mirror with modest degradation of the straylight performance.

Dinger, Ann S.↗

Spectral Properties of Dust on Asteroid (101955) Bennu with the OSIRIS-REx Visible and Infrared Spectrometer (OVIRS)

The Origins, Spectral Interpretation, Resource Identification, Security-Regolith Explorer (OSIRIS-REx) spacecraft successfully collected a sample of regolith from asteroid (101955) Bennu on October 20, 2020[1]. The Touch-and-Go (TAG) spacecraft maneuver used to collect the sample mobilized material, including very fine particles (dust),from up to ~1m in depth and ~10m in diameter from the point of contact with the asteroid [1,2].TAG created a dust plume that intercepted the spacecraft and accumulated on the instruments. As a result, all instruments show degradation in optical throughput, with the degree of degradation depending on their aperture size, orientation, and position on the spacecraft [1]. In this study, we analyzed OSIRIS-REx Visible and Infrared Spectrometer (OVIRS) from before and after TAG to assess the dust on the instrument with implications for Bennu’s dust composition and scattering properties, and future visible–near-infrared (VNIR)spectral observations with the OSIRIS-REx spacecraft. OVIRS is a point spectrometer that measures reflected light at VNIR wavelengths from 0.4 to 4.3 μm with a 4 mrad circular field of view(FOV) [3]. Previous observations with OVIRS revealed widespread hydrated minerals and carbon-bearing materials on Bennu [4,5]. A ~3 μm absorption feature is observed globally in OVIRS spectra, with a band position and depth that is consistent with Mg/Fe phyllosilicates and suggests a similar degree of aqueous alteration to that experienced by some carbonaceous chondrite meteorites[4,6]. A series of weak (<5%) VNIR spectral features, potentially associated with hydrated phyllosilicates and iron oxides, are also found in OVIRS spectra (Fig. 1), though not typically observed in meteorite spectra [7]. OSIRIS-REx Thermal Emission Spectrometer (OTES) spectra are consistent with a very thin accumulation (a few to ~ten microns) of fine particles (<~65–100 microns in size)across the surface of Bennu, and particularly on the roughest boulders, an interpretation that is supported by the dust plume seen during TAG [1,8]. An analysis of TAG contamination on the OTES optics revealed a 15% decrease in throughput, and OTES observations of space with and without that contamination allow a recreation of the thermal infrared spectrum [1]. The spectrum of the contamination lacks volume scattering features and exhibits a stronger Mg-OH absorption (~16.5 μm) than average Bennu, suggesting a Mg-rich phyllosilicate composition [1,6]. Here we present the first compositional analysis of Bennu dust at VNIR wavelengths by analyzing the contaminant on OVIRS.

H H Kaplan↗

Scatter from optical components; Proceedings of the Meeting, San Diego, CA, Aug. 8-10, 1989

Various papers on scatter from optical components are presented. Individual topics addressed include: BRDF of SiC and Al foam compared to black paint at 3.39 microns, characterization of optical baffle materials, bidirectional transmittance distribution function of several IR materials at 3.39 microns, thermal cycling effects on the BRDF of beryllium mirrors, BTDV of ZnSe with multilayer coatings at 3.39 microns, scattering from contaminated surfaces, cleanliness correlation by BRDF and PFO instruments, contamination effects on optical surfaces, means of eliminating the effects of particulate contamination on scatter measurements of superfine optical surfaces, vacuum BRDF measurement of cryogenic optical surfaces, Monte Carlo simulation of contaminant transport to and deposition on complex spacecraft surfaces, surface particle observation and BRDF predictions, satellite material contaminant optical properties, dark field photographic techniques for documenting optical surface contamination, design of a laboratory study of contaminant film darkening in space, contamination monitoring approaches for EUV space optics.

Stover, John C.↗

Design and characterization of a low cost CubeSat multi-band optical receiver to map water ice on the lunar surface for the Lunar Flashlight mission

Lunar Flashlight is an innovative NASA CubeSat mission dedicated to mapping water ice in the permanently shadowed regions of the Moon, which may act as cold traps for volatiles. To this end, a multi-band reflectometer will be sent to orbit the Moon. This instrument consists of an optical receiver aligned with four lasers, each of which emits sequentially at a different wavelength in the near-infrared between 1 μm and 2 μm. The receiver measures the laser light reflected from the lunar surface; continuum/absorption band ratios are then analyzed to quantify water ice in the illuminated spot. Here, we present the current state of the optical receiver design. To optimize the optical signal-to-noise ratio, we have designed the receiver so as to maximize the laser signal collected, while minimizing the stray light reaching the detector from solar-illuminated areas of the lunar surface outside the field-of-view, taking into account the complex lunar topography. Characterization plans are also discussed. This highly mass- and volume-constrained mission will demonstrate several firsts, including being one of the first CubeSats performing science measurements beyond low Earth orbit.

Sellar, Glenn R.↗

Compact, All-PM Fiber Integrated and Alignment-Free Ultrafast Yb:Fiber NALM Laser With Sub-Femtosecond Timing Jitter

We report a simple and compact design of a dispersion compensated mode-locked Yb:fiber oscillator based on a nonlinear amplifying loop mirror (NALM). The fully polarization maintaining (PM) fiber integrated laser features a chirped fiber Bragg grating (CFBG) for dispersion compensation and a fiber integrated compact non-reciprocal phase bias device, which is alignment-free. The main design parameters were determined by numerically simulating the pulse evolution in the oscillator and by analyzing their impact on the laser performance. Experimentally, we achieved an 88 fs compressed pulse duration with sub-fs timing jitter at 54 MHz repetition rate and 51 mW of output power with 5.5×10 -5 [20 Hz, 1 MHz] integrated relative intensity noise (RIN). Furthermore, we demonstrate tight phase-locking of the laser's carrier-envelope offset frequency ( fceo ) to a stable radio frequency (RF) reference and of one frequency comb tooth to a stable optical reference at 291 THz.

47 OTHER INSTRUMENTATION↗

The Chandra X-Ray Observatory-Overview and Status

The Chandra X-Ray Observatory was launched early in the morning of 1999, July 23 by the Space Shuttle Columbia. The Shuttle launch was only the first step in placing NASA's latest great observatory into orbit. After release from the cargo bay, the Inertial Upper Stage performed two firings and separated from the observatory as planned. Finally, after five firings of Chandra's own Integral Propulsion System - the last of which took place 15 days after the initial launch - the observatory was placed in its highly elliptical orbit of 140,000 km apogee and 10,000 km perigee. After Observatory activation, the first x-rays focussed by the telescope were observed on 1999, August 12. Beginning with this initial observation one could conclude that the telescope had survived the launch environment and was operating as expected. The month following the opening of the sunshade door was spent adjusting the focus for each set of instrument configurations, determining the optical axis, calibrating the star camera, establishing the relative response functions, determining the energy scale(s), and performing a series of "publicity" images. Each observation proved to be far more revealing than was expected. Preliminary results will be presented and the status of the instrumentation on the observatory will be discussed.

Weisskopf, Martin C.↗

The Chandra X-Ray Observatory Overview

The Chandra X-Ray Observatory was launched early in the morning of July 23, 1999 by the Space Shuttle Columbia. The Shuttle launch was only the first step in placing NASA's latest great observatory into orbit. After release from the cargo bay, the Inertial Upper Stage performed two firings and separated from the observatory as planned. Finally, after five firings of Chandra's own Integral Propulsion System - the last of which took place 15 days after the initial launch - the observatory was placed in its highly elliptical orbit of 140,000 km apogee and 10,000 km perigee. After Observatory activation, the first x-rays focussed by the telescope were observed on August 12, 1999. Beginning with this initial observation one could conclude that the telescope had survived the launch environment and was operating as expected. The month following the opening of the sunshade door was spent adjusting the focus for each set of instrument configurations, determining the optical axis, calibrating the star camera, establishing the relative response functions, determining the energy scale(s), and performing a series of "publicity" images. Each observation proved to be far more revealing than was expected. Preliminary results will be presented and the status of the instrumentation on the observatory will be discussed.

Weisskopf, Martin C.↗

The Chandra X-Ray Observatory: An Overview

The Chandra X-Ray Observatory was launched early in the morning of July 23, 1999, by the Space Shuttle Columbia. The Shuttle launch was only the first step in placing NASA's latest great observatory into orbit. After release from the cargo bay, the Inertial Upper Stage performed two firings and separated from the observatory as planned. Finally, after five firings of Chandra's own Integral Propulsion System - the last of which took place 15 days after the initial launch - the observatory was placed in its highly elliptical orbit of 140,000 km apogee and 10,000 km perigee. After Observatory activation, the first x-rays focussed by the telescope were observed on 1999, August 12. Beginning with this initial observation one could conclude that the telescope had survived the launch environment and was operating as expected. The month following the opening of the sunshade door was spent adjusting the focus for each set of instrument configurations, determining the optical axis, calibrating the star camera, establishing the relative response functions, determining the energy scale(s), and performing a series of "publicity" images. Each observation proved to be far more revealing than was expected. Preliminary results will be presented and the status of the instrumentation on the observatory will be discussed.

Weisskopf, Martin C.↗

The Chandra X-Ray Observatory Overview

The Chandra X-Ray Observatory was launched early in the morning of July 23, 1999 by the Space Shuttle Columbia. The Shuttle launch was only the first step in placing NASA's latest great observatory into orbit. After release from the cargo bay, the Inertial Upper Stage performed two firings and separated from the observatory as planned. Finally, after five firings of Chandra's own Integral Propulsion System - the last of which took place 15 days after the initial launch - the observatory was placed in its highly elliptical orbit of 140,000 km apogee and 10,000 km perigee. After Observatory activation, the first x-rays focused by the telescope were observed on August 12, 1999. Beginning with this initial observation one could conclude that the telescope had survived the launch environment and was operating as expected. The month following the opening of the sunshade door was spent adjusting the focus for each set of instrument configurations, determining the optical axis, calibrating the star camera, establishing the relative response functions, determining the energy scale(s), and performing a series of "publicity" images. Each observation proved to be far more revealing than was expected. Preliminary results will be presented and the status of the instrumentation on the observatory will be discussed.

Weisskopf, Martin C.↗

Development of a Self-Aligned Compact Focusing Schlieren System for NASA Test Facilities

A novel compact, inherently self-aligned focusing schlieren system is presented for use in experiments that require flow visualization. Unlike existing focusing schlieren systems that require a separate, and precisely aligned, source grid and cutoff grid to achieve sensitivity to density gradients, this system projects an image of a physical grid element onto a background and then reimages this projection back onto the physical grid element thereby achieving self-alignment. Further, unlike existing focusing schlieren systems where the image of the source grid is physically translated relative to the cutoff grid to achieve sensitivity to density gradients, a polarization prism in the system acts on the polarization state of the light of the projected grid element to provide sensitivity adjustment. By adjusting the position of the prism along the optical axis of the instrument, changes to the sensitivity can be realized. Since the outgoing and returning light used to project an image of the grid element are orthogonally polarized to one another, reflections from windows that may be present between the lens and background of the system can be eliminated. The system can also be implemented in test facilities where optical access is limited and/or exists on only one side. A discussion on different aspects of the development of this technology is provided, as are sample images from the system at the different stages of development. Images that demonstrate the ability to make on-the-fly adjustments to the instrument's field-of-view and working distance are also presented.

Brett F Bathel↗