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At least 1,153 records · Page 64
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Alteration of Rock Fragments from Columbia River Basalt Microcosms

During an earlier study, microorganisms were grown microcosms consisting of sterilized chips of Columbia River Basalt (CRB) and natural CRB ground water with its natural microflora; environmental conditions simulated a deep subsurface, anaerobic, dark environment. Subsequent scanning and transmission electron microscope (SEM and TEM) studies revealed the presence of several types of bacteria and biofilm, some of which were mineralized. Some of these biological features are very similar to possible biogenic features found in two meteorites from Mars, ALH84001 (found in Antarctica) and Nakhla (observed to fall in Egypt). Both ALH84001 and Nakhla contain traces of low-temperature aqueous alteration of silicates, oxides, and sulfides. The goals of this study are to use high-resolution field-emission SEM (FE-SEM) to examine the CRB samples for evidence of alteration features similar to those in the martian meteorites, to determine the extent of alteration during the CRB microcosm experiments, and to determine whether effects of biological activity can be distinguished from inorganic effects.

Wentworth, Susan J.↗
NASA NTRS1999

Wheat Response to Differences In Water and Nutritional Status Between Zeoponic and Hydroponic Growth Systems

Hydroponic culture has traditionally been used for controlled environment life support systems (CELSS) because the optimal environment for roots supports high growth rates. Recent developments in zeoponic substrate and microporous tube irrigation (ZPT) also offer high control of the root environment. This study compared the effect of differences in water and nutrient status of ZPT or hydroponic culture on growth and yield of wheat (Triticum aestivum L., CV 'USU-Apogee'). In a side-by-side test in a controlled environment, wheat was grown in ZPT and recirculating hydroponics to maturity. Water use by plants grown in both culture systems peaked at 15-20 L per square meters per d up to day 40, after which it declined more rapidly for plants grown in ZPT culture due to earlier senescence of leaves. No consistent differences were noted in water status between plants grown in the two culture systems. Although yield was similar, harvest index was 28% lower for plants grown in ZPT versus hydroponic culture. Sterile green tillers made up 12% and 0% of the biomass of plants grown in ZPT and hydroponic culture, respectively. Differences in biomass partitioning were attributed primarily to NH4 -N nutrition of plants grown in ZPT as compared with NO3-N in hydroponic nutrient solution. It was likely that NH4-N induced Ca deficiency produced excess tillering and lower harvest index for plants grown in ZPT culture. These results suggest that further refinements in zeoponic substrate would make ZPT culture a viable alternative for achieving high productivity in a CELSS.

Steinberg, Susan L.↗
NASA NTRS2000

The Turbulent Life of Phytoplankton

Phytoplankton is a generic name for photosynthesizing microscopic organisms that inhabit the upper sunlit layer (euphotic zone) of almost all oceans and bodies of freshwater. They are agents for "primary production," the incorporation of carbon from the environment into living organisms, a process that, sustains the aquatic food web. It is estimated that phytoplankton contribute about half of the global primary production, the other half being due to terrestrial plants. By sustaining the aquatic food web and controlling the biogeochemical cycles through primary production, phytoplankton exert a dominant influence on life on earth. Turbulence influences this process in three very important ways. First, essential mineral nutrients are transported from the deeper layers to the euphotic zone through turbulence. Second, turbulence helps to suspend phytoplankton in the euphotic zone since in still water, the phytoplankton, especially the larger species, tend to settle out of the sunlit layers. Third, turbulence transports phytoplankton from the surface to the dark sterile waters, and this is an important mechanism of loss. Thus, stable phytoplankton populations are maintained through a delicate dynamic balance between the processes of turbulence, reproduction, and sinking. The first quantitative model for this was introduced by Riley, Stommel and Bumpus in 1949. This is an attempt to extend their efforts through a combination of analysis and computer simulation in order to better understand the principal qualitative aspects of the physical/biological coupling of this natural system.

S Ghosal↗
NASA NTRS2000

Overview of Mars Sample Hazard Analysis (Requirements Workshop Series)

Samples returned from Mars should be contained and treated as though potentially hazardous until proven otherwise. If sample containment cannot be verified en route to Earth, the sample and spacecraft should either be sterilized in space or not returned to Earth. Integrity of sample containment should be maintained through reentry and transfer to a receiving facility. Controlled distribution of unsterilized materials should only occur if analyses determine the sample not to contain a biological hazard. Planetary protection measures adopted for the first sample return should not be relaxed for subsequent missions without thorough scientific review and concurrence by an appropriate independent body.

Rummel, John D.↗
NASA NTRS2000

The Effect of Dilution on the Structure of Microbial Communities

To determine how dilution of microbial communities affects the diversity of the diluted assemblage a series of numerical simulations were conducted that determined the theoretical change in diversity, richness, and evenness of the community with serial dilution. The results of the simulation suggested that the effects are non linear with a high degree of dependence on the initial evenness of the community being diluted. A series of incubation experiments using a range of dilutions of raw sewage as an inoculum into sterile sewage was used for comparison to the simulations. The diluted communities were maintained in batch fed reactors (three day retention time) for nine days. The communities were harvested and examined by conventional plating and by molecular analysis of the whole-community DNA using AFLP and T-RFLP. Additional, CLPP analysis was also applied. The effects on richness predicted by the numerical simulations were confirmed by the analyses used. The diluted communities fell into three groups, a low dilution, intermediate dilution, and high dilution group, which corresponded well with the groupings obtained for community richness in simulation. The grouping demonstrated the non-linear nature of dilution of whole communities. Furthermore, the results implied that the undiluted community consisted of a few dominant types accompanied by a number of rare (low abundance) types as is typical in unevenly distributed communities.

Mills, Aaron L.↗
NASA NTRS1994

The Use of Microwave Incineration to Process Biological Wastes

The handling and disposal of solid waste matter that has biological or biohazardous components is a difficult issue for hospitals, research laboratories, and industry. NASA faces the same challenge as it is developing regenerative systems that will process waste materials into materials that can be used to sustain humans living in space for extended durations. Plants provide critical functions in such a regenerative life support scheme in that they photosynthesize carbon dioxide and water into glucose and oxygen. The edible portions of the plant provide a food source for the crew. Inedible portions can be processed into materials that are more recyclable. The Advanced Life Support Division at NASA Ames Research Center has been evaluating a microwave incinerator that will oxidize inedible plant matter into carbon dioxide and water. The commercially available microwave incinerator is produced by Matsushita Electronic Instruments Corporation of Japan. Microwave incineration is a technology that is simple, safe, and compact enough for home use. It also has potential applications for institutions that produce biological or biohazardous waste. The incinerator produces a sterile ash that has only 13% of the mass of the original waste. The authors have run several sets of tests with the incinerator to establish its viability in processing biological material. One goal of the tests is to show that the incinerator does not generate toxic compounds as a byproduct of the combustion process. This paper will describe the results of the tests, including analyses of the resulting ash and exhaust gases. The significance of the results and their implications on commercial applications of the technology will also be discussed.

Sun, Sidney C.↗
NASA NTRS1995

Contributions of Planetary Science to Studies of Early Biosphere Evolution

The history of impact cratering on the Moon, and extrapolations of crater chronologies to the inner planets, suggests that the late accretionary history of the Earth overlapped with other crucial events in the its history, including the origin of terrestrial life. This evidence, acquired from studies of other planetary bodies in the inner solar system, has profoundly affected how we view the early history of the Earth and evolution of the biosphere. Pre-biotic chemical evolution and the origin of life would have been delayed by the probable existence of a global magma ocean until -4.2 Ga. The early crust was largely destroyed by recycling, thus accounting for the sparse Archean record on Earth. Once life had developed, large impacts may have extinguished it several times before it finally gained a foothold. Potentially sterilizing impacts could have occurred as late as 3.7 Ga. At the very least, large impacts would have forced the biosphere through major environmental "bottlenecks" thereby canalizing its subsequent evolution. One legacy of these early events may be the structure of the present RNA-tree which indicates that extreme thermophiles are primitive within the Archaea, and may be the last common ancestors of life. By 3.5 Ga, marine sedimentary sequences contain unequivocal microbial fossils that attest to the presence of a terrestrial biosphere. The diversity of microbial forms present in these earliest fossil assemblages implies a preceding interval of evolution during which major evolutionary advances (e.g. photosynthesis) could have taken place. Evidence cited above places the origin of life within the interval 3.5 and 4.2 Ga, a period of 700 Ma. Thus, it appears that terrestrial life not only evolved rapidly, but perhaps more than once. This expands the possibilities that life may have also developed elsewhere. Of the other planets in our solar system, Mars holds the greatest chance of having developed life. But, the present surface of Mars is hostile to life. Liquid water, regarded as essential for living systems, is unstable on the surface of Mars due to the low atmospheric pressure. The results of the Viking Lander biology experiments established that organic molecules are not present in the regolith of Mars, forcing the exobiological community to consider new ways of exploring for Martian life. Older, heavily cratered terranes on Mars show geomorphic evidence for abundant water between 3.0- 4.0 Ga. It is quite possible that life developed on Mars during this time, as it did on the Earth. The present focus for Mars Exobiology lies in the search for a fossil record. Archean-aged crust, while mostly missing on Earth, appears to be widespread in ancient cratered highlands of Mars, and aqueous mineral deposits within such sequences may hold crucial fossil evidence for an early Martian biosphere.

Farmer, Jack D.↗
NASA NTRS2001

Introduction to Mars Sample Handling Workshop Series: Overview of Mars Sample Hazard Analysis

Samples returned from Mars should be contained and treated as though potentially hazardous until proven otherwise If sample containment cannot be verified en route to Earth, the sample and spacecraft should either be sterilized in space or not returned to Earth. Integrity of sample containment should be maintained through reentry and transfer to a receiving facility. Controlled distribution of unsterilized materials should only occur if analyses determine the sample not to contain a biological hazard. Planetary protection measures adopted for the first sample return should not be relaxed for subsequent missions without thorough scientific review and concurrence by an appropriate independent body

Rummel, John D.↗
NASA NTRS1992

Precision Heating Process

A heat sealing process was developed by SEBRA based on technology that originated in work with NASA's Jet Propulsion Laboratory. The project involved connecting and transferring blood and fluids between sterile plastic containers while maintaining a closed system. SEBRA markets the PIRF Process to manufacturers of medical catheters. It is a precisely controlled method of heating thermoplastic materials in a mold to form or weld catheters and other products. The process offers advantages in fast, precise welding or shape forming of catheters as well as applications in a variety of other industries.

Source record↗
NASA NTRS1983

Contamination Control

Upjohn Company sought a solution to their problem of potential particulate contamination of sterile injectable drugs. Contamination was caused by dust particles attracted by static electrical charge, which clung to plastic curtains in clean rooms. Upjohn found guidance in NASA Tech Briefs which provided detailed information for reducing static electricity. Guidelines for setting up static free work stations, materials and equipment needed to maintain antistatic protection.

Source record↗
NASA NTRS1982

Water Filter

A compact, lightweight electrolytic water sterilizer available through Ambassador Marketing, generates silver ions in concentrations of 50 to 100 parts per billion in water flow system. The silver ions serve as an effective bactericide/deodorizer. Tap water passes through filtering element of silver that has been chemically plated onto activated carbon. The silver inhibits bacterial growth and the activated carbon removes objectionable tastes and odors caused by addition of chlorine and other chemicals in municipal water supply. The three models available are a kitchen unit, a "Tourister" unit for portable use while traveling and a refrigerator unit that attaches to the ice cube water line. A filter will treat 5,000 to 10,000 gallons of water.

Source record↗
NASA NTRS1976

Biological Isolation Garment

A spinoff of astronaut's biological garment will allow hospital patients who are highly vulnerable to infection to leave their sterile habitats for several hours, carrying their germ free environment with them. Garments can be used in any of some 200 hospitals where isolation rooms are installed to treat leukemia.

Source record↗
NASA NTRS2003

Planetary Protection Considerations for JIMO

Europa is the only body in the solar system besides Mars that is currently viewed as a body of significant interest relative to the process of chemical evolution and/or the origin of life or for which scientific opinion provides a significant chance of contamination which could jeopardize a future biological experiment. Thus, both NASA and COSPAR policy require that Europa be protected from biological contamination that could result from scientific exploration conducted by robotic spacecraft. In 2000, the Task Group on the Forward Contamination of Europa (Space Studies Board) published its report on Preventing the Forward Contamination of Europa recommending a limit of 10(exp -4) probability of contamination of Europa's ocean per mission (at any time in the future) by a single viable terrestrial microbe. While NASA guidelines do not yet explicitly reflect this new recommendation, it is likely that the SSB recommendation will be adopted by NASA planetary protection in the form of a sterility requirement or at least a stringent total microbial burden requirement. In our presentation, we will present an overview of the anticipated planetary protection requirements for both orbiters and landers destined for Europa and some of the challenges these requirements will present.

Koukol, R. C.↗
NASA NTRS2003

Removal of Biologically Active Organic Contaminants using Atomic Oxygen

Biomedical devices that are to come into contact with living tissue, such as prosthetic and other implants for the human body and the containers used to store and transport them, are together cleaned of non-living, but biologically active organic materials, including endotoxins such as lipopolysaccharides, and assembled into a hermetically sealed package without recontamination. This is achieved by cleaning both the device and package components together in an apparatus, which includes a hermetically sealed chamber, in which they are contacted with atomic oxygen which biocleans them, by oxidizing the biologically active organic materials. The apparatus also includes means for manipulating the device and container and hermetically sealing the cleaned device into the cleaned container to form the package. A calibrated witness coupon visually indicates whether or not the device and container have received enough exposure to the atomic oxygen to have removed the organic materials from their surfaces. Gamma radiation is then used to sterilize the device in the sealed container.

Bruce A Banks↗
NASA NTRS2002

Comparative Results of Using Different Methods for Discovery of Microorganisms in very Ancient Layers of the Central Antarctic Glacier above the Lake Vostok

The ice sheet of the Central Antarctic is considered by the scientific community worldwide, as a model to elaborate on different methods to search for life outside Earth. This became especially significant in connection with the discovery of the underglacial lake in the vicinity of the Russian Antarctic Station Vostok. Lake Vostok is considered by many scientists as an analog of the ice covered seas of Jupiter's satellite Europa. According to the opinion of many researchers there is the possibility that relict forms of microorganisms, well preserved since the Ice Age, may be present in this lake. Investigations throughout the thickness of the ice sheet above Lake Vostok show the presence of microorganisms belonging to different well-known taxonomic groups, even in the very ancient horizons near close to floor of the glacier. Different methods were used to search for microorganisms that are rarely found in the deep ancient layers of an ice sheet. The method of aseptic sampling from the ice cores and the results of controlled sterile conditions in all stages when conducting these investigations, are described in detail in previous reports. Primary investigations tried the usual methods of sowing samples onto different nutrient media, and the result was that only a few microorganisms grew on the media used. The possibility of isolating the organisms obtained for further investigations, by using modern methods including DNA-analysis, appears to be the preferred method. Further investigations of the very ancient layers of the ice sheet by radioisotopic, luminescence, and scanning electron microscopy methods at different modifications, revealed the quantity and morphological diversity of the cells of microorganisms that were distributed on the different horizons. Investigations over many years have shown that the microflora in the very ancient strata of the Antarctic ice cover, nearest to the bedrock, support the effectiveness of using a combination of different methods to search for signs of life in ancient icy formations, which might play a role in the long-term preservation and transportation of microbial life throughout the Universe.

Abyzov, S. S.↗
NASA NTRS2002

Oxygen Penalty for Waste Oxidation in an Advanced Life Support System: A Systems Approach

Oxidation is one of a number of technologies that are being considered for waste management and resource recovery from waste materials generated on board space missions. Oxidation processes are a very effective and efficient means of clean and complete conversion of waste materials to sterile products. However, because oxidation uses oxygen there is an "oxygen penalty" associated either with resupply of oxygen or with recycling oxygen from some other source. This paper is a systems approach to the issue of oxygen penalty in life support systems and presents findings on the oxygen penalty associated with an integrated oxidation-Sabatier-Oxygen Generation System (OGS) for waste management in an Advanced Life Support System. The findings reveal that such an integrated system can be operated to form a variety of useful products without a significant oxygen penalty.

Pisharody, Suresh↗
NASA NTRS2002

Requirements Development Issues for Advanced Life Support Systems: Solid Waste Management

Long duration missions pose substantial new challenges for solid waste management in Advanced Life Support (ALS) systems. These possibly include storing large volumes of waste material in a safe manner, rendering wastes stable or sterilized for extended periods of time, and/or processing wastes for recovery of vital resources. This is further complicated because future missions remain ill-defined with respect to waste stream quantity, composition and generation schedule. Without definitive knowledge of this information, development of requirements is hampered. Additionally, even if waste streams were well characterized, other operational and processing needs require clarification (e.g. resource recovery requirements, planetary protection constraints). Therefore, the development of solid waste management (SWM) subsystem requirements for long duration space missions is an inherently uncertain, complex and iterative process. The intent of this paper is to address some of the difficulties in writing requirements for missions that are not completely defined. This paper discusses an approach and motivation for ALS SWM requirements development, the characteristics of effective requirements, and the presence of those characteristics in requirements that are developed for uncertain missions. Associated drivers for life support system technological capability are also presented. A general means of requirements forecasting is discussed, including successive modification of requirements and the need to consider requirements integration among subsystems.

Levri, Julie A.↗
NASA NTRS2003

Survival of endospores of Bacillus subtilis on spacecraft surfaces under simulated martian environments: implications for the forward contamination of Mars

Experiments were conducted in a Mars simulation chamber (MSC) to characterize the survival of endospores of Bacillus subtilis under high UV irradiation and simulated martian conditions. The MSC was used to create Mars surface environments in which pressure (8.5 mb), temperature (-80, -40, -10, or +23 degrees C), gas composition (Earth-normal N2/O2 mix, pure N2, pure CO2, or a Mars gas mix), and UV-VIS-NIR fluence rates (200-1200 nm) were maintained within tight limits. The Mars gas mix was composed of CO2 (95.3%), N2 (2.7%), Ar (1.7%), O2 (0.2%), and water vapor (0.03%). Experiments were conducted to measure the effects of pressure, gas composition, and temperature alone or in combination with Mars-normal UV-VIS-NIR light environments. Endospores of B. subtilis, were deposited on aluminum coupons as monolayers in which the average density applied to coupons was 2.47 x 10(6) bacteria per sample. Populations of B. subtilis placed on aluminum coupons and subjected to an Earth-normal temperature (23 degrees C), pressure (1013 mb), and gas mix (normal N2/O2 ratio) but illuminated with a Mars-normal UV-VIS-NIR spectrum were reduced by over 99.9% after 30 sec exposure to Mars-normal UV fluence rates. However, it required at least 15 min of Mars-normal UV exposure to reduce bacterial populations on aluminum coupons to non-recoverable levels. These results were duplicated when bacteria were exposed to Mars-normal environments of temperature (-10 degrees C), pressure (8.5 mb), gas composition (pure CO2), and UV fluence rates. In other experiments, results indicated that the gas composition of the atmosphere and the temperature of the bacterial monolayers at the time of Mars UV exposure had no effects on the survival of bacterial endospores. But Mars-normal pressures (8.5 mb) were found to reduce survival by approximately 20-35% compared to Earth-normal pressures (1013 mb). The primary implications of these results are (a) that greater than 99.9% of bacterial populations on sun-exposed surfaces of spacecraft are likely to be inactivated within a few tens of seconds to a few minutes on the surface of Mars, and (b) that within a single Mars day under clear-sky conditions bacterial populations on sun-exposed surfaces of spacecraft will be sterilized. Furthermore, these results suggest that the high UV fluence rates on the martian surface can be an important resource in minimizing the forward contamination of Mars. c2003 Elsevier Inc. All rights reserved.

Bacillus subtilis/growth & development/physiology/↗